RESUMO
A multi-joystick robotic laser microscope system used to control two optical traps (tweezers) and one laser scissors has been developed for subcellular organelle manipulation. The use of joysticks has provided a "user-friendly" method for both trapping and cutting of organelles such as chromosomes in live cells. This innovative design has enabled the clean severing of chromosome arms using the laser scissors as well as the ability to easily hold and pull the severed arm using the laser tweezers.
Assuntos
Cromossomos , Micromanipulação/instrumentação , Pinças Ópticas , Animais , Linhagem Celular , Desenho de Equipamento , Camundongos , Micromanipulação/métodos , Micromanipulação/estatística & dados numéricos , Mitose , Fenômenos Ópticos , Potoroidae , SoftwareRESUMO
Angular motion of a hand-held instrument due to physiological tremor during micromanipulation tasks was recorded with a six degree-of-freedom accelerometer-based sensing unit placed in the instrument. Methods to get angular velocities and angular accelerations from the acceleration readings of accelerators in the sensing unit are described. Statistics of angular velocity and angular acceleration of the instrument due to the tremor obtained from ten normal subjects are reported. Assumption of very small tremor angular velocities in micromanipulation tasks to calculate tremor angular accelerations analytically is validated.
Assuntos
Micromanipulação/instrumentação , Micromanipulação/estatística & dados numéricos , Tremor/fisiopatologia , Aceleração , Engenharia Biomédica , Humanos , Microcirurgia/instrumentação , Microcirurgia/estatística & dados numéricos , Modelos Biológicos , Modelos Teóricos , Movimento (Física) , Instrumentos CirúrgicosRESUMO
OBJECTIVE: To evaluate whether assisted hatching improves clinical outcomes of embryo transfers to good prognosis patients, defined as patients < or =39 years with normal follicle-stimulating hormone (FSH) and E(2) levels, no more than one previous unsuccessful cycle of in vitro fertilization (IVF)-embryo transfer, and good embryo quality. DESIGN: Prospective randomized controlled trial. SETTING: Private assisted reproductive technology (ART) center. PATIENT(S): One hundred ninety-nine good prognosis patients undergoing IVF-embryo transfer. INTERVENTION(S): In vitro fertilization followed by embryo transfer on day 3 after oocyte retrieval with or without assisted hatching using a 1,480-nm wavelength infrared laser. MAIN OUTCOME MEASURE(S): Clinical intrauterine pregnancy, spontaneous pregnancy loss, and live birth. RESULT(S): Rates of clinical intrauterine pregnancy with fetal cardiac activity (53% vs. 54% per cycle), spontaneous pregnancy loss (13% vs. 16% per pregnancy), and live birth (47% vs. 46% per cycle) were very similar between treatment cycles with laser-assisted hatching and control cycles in which embryos were transferred without assisted hatching. There were no significant differences between treatment and control groups in any measured clinical outcome parameters. CONCLUSION(S): Assisted hatching does not improve clinical outcomes among good prognosis patients.
Assuntos
Transferência Embrionária/estatística & dados numéricos , Fertilização in vitro/estatística & dados numéricos , Infertilidade Feminina/epidemiologia , Infertilidade Feminina/terapia , Terapia a Laser , Microdissecção/estatística & dados numéricos , Resultado da Gravidez/epidemiologia , Adulto , Feminino , Humanos , Maryland/epidemiologia , Microdissecção/métodos , Micromanipulação/métodos , Micromanipulação/estatística & dados numéricos , Avaliação de Resultados em Cuidados de Saúde , Gravidez , Prognóstico , Resultado do TratamentoRESUMO
To determine the impact of multiple micromanipulation procedures for preimplantation genetic diagnosis (PGD) on embryo development, a retrospective analysis was performed of 9,925 embryos (862 PGD cycles), which were compared with 28,126 nonbiopsied embryos (2,751 consecutive intracytoplasmic sperm injection [ICSI] cycles) from the same time period. Because fertilization rates, the proportion of embryos with > or = 6 cells on day 3, and blastocyst rates were similar in the PGD and control groups, we conclude that multiple micromanipulations on oocytes and embryos can be performed safely for PGD.