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1.
Food Chem ; 337: 128007, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32919278

RESUMO

The purpose of this study is to evaluate the bioaccessibility of nutrients and antioxidant activity of O. radicata after subjecting to four types of domestic cooking and followed by in vitro digestion. The result demonstrated that the group with the lowest amino acid release and the degree of protein hydrolysis (5.6%) was frying, but both reducing sugar content and antioxidant activity were the highest. The composition of fatty acids was different than undigested samples, especially the relative content of linolenic acid was significantly decreased (e.g., 34.49 to 8.23%, boiled). The difference of the minerals bioaccessibility was slightly affected by the cooking method, but mainly related to their natural properties, such as the highest phosphorus (62.73%) and the lowest iron (21.53%) in the steaming. The above data provides a starting point for the design of processes at an industrial and gastronomic level.


Assuntos
Agaricales/química , Antioxidantes/análise , Culinária/métodos , Nutrientes/farmacocinética , Disponibilidade Biológica , Carboidratos/farmacocinética , Digestão , Minerais/farmacocinética , Vapor
2.
PLoS One ; 15(5): e0232237, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32357196

RESUMO

PURPOSE: A study was made of the changes in gene expression in elite handball athletes, comparing gene modulation before, after and in the absence of an 8-week nutritional intervention with multivitamin/mineral supplements. METHODS: Thirteen elite handball athletes (aged 22.9 ± 2.7 years) and 13 sedentary controls (aged 20.9 ± 2.8 years) were included. Three timepoints were established: T0 (baseline conditions); T8 (after 8 weeks of supplementation with a multivitamin/mineral complex); and T16 (after 8 weeks in the absence of supplementation). The expressions of a total 112 of genes were evaluated by RT-qPCR analysis with the QuantStudioTM 12K Flex Real-Time PCR System. RESULTS: The analysis revealed different gene regulation profiles of genes implicated in cell communication, cell energy metabolism, inflammation and the immune system, oxidative stress and muscle function in athletes compared to sedentary controls under resting conditions (upregulated genes: effect size = large, ƞ2 = 1.011 to 1.398, p < 0.05; downregulated genes: effect size = large, ƞ2 = 0.846 and 1.070, p < 0.05, respectively). The nutritional intervention encouraged gene upregulation in elite athletes (p < 0.05). In a follow-up investigation, the IRAK1, CD81, ITGB1, ACADS PDHA2 and GPX1 genes were downregulated in athletes, with a moderate main effect for time-by-group interaction (ηP2 = 0.099 to 0.133; p < 0.05). Additionally, nutritional genes such as MTHFR and THTPA revealed a moderate effect over all the timepoints and group interaction in the study (ηP2 = 0.070 to 0.092; p < 0.05). CONCLUSIONS: Elite handball athletes showed a different expression profile in reference to key genes implicated in several sports performance-related functions compared to the sedentary controls, in addition to modulation of gene expression after multivitamin/mineral supplementation.


Assuntos
Atletas , Desempenho Atlético , Suplementos Nutricionais , Micronutrientes/farmacologia , Minerais/farmacocinética , Transcriptoma/efeitos dos fármacos , Vitaminas/farmacologia , Adulto , Humanos , Masculino , Reação em Cadeia da Polimerase em Tempo Real , Adulto Jovem
3.
IET Nanobiotechnol ; 13(7): 756-765, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31573546

RESUMO

This study describes the preparation, characterisation and application of pelletised immobilised alginate/montmorillonite/banana peels nanocomposite (BPNC) in a fixed-bed column for continuous adsorption of rare earth elements and radioactive minerals from water. The materials was characterised by Fourier transform infrared, X-ray diffraction and scanning electron microscopy analyses. Analyses indicated that the pellets are porous and spherical in shape. FT-IR analysis showed that the functional groups responsible for the coordination of metal ions were the carboxylic (-COO-) and siloxane (Si-O-Si and Si-O-Al) groups. XRD analysis showed two additional peaks which were attributed to alginate and montmorillonite. The influence of the initial concentration, bed depth and flow rate were investigated using synthetic and real mine water in order to determine the breakthrough behaviour of both minerals. The processed bed volume, adsorbent exhaustion rate and service time, were also explored as performance indices for the adsorbent material. Furthermore, the breakthrough data were fitted to both the Thomas and Bohart-Adams models. The BPNC exhibited high affinity for U, Th, Gd and La in the real mine water sample. However, studies may still be required using waters from different environments in order to determine the robustness of BPNC.


Assuntos
Alginatos/síntese química , Elementos Radioativos/isolamento & purificação , Metais Terras Raras/isolamento & purificação , Mineração , Musa/química , Nanocompostos , Purificação da Água/métodos , Adsorção , Alginatos/química , Alginatos/farmacocinética , Elementos Radioativos/farmacocinética , Química Verde , Humanos , Teste de Materiais , Metais Terras Raras/farmacocinética , Minerais/isolamento & purificação , Minerais/farmacocinética , Mineração/métodos , Nanocompostos/química , Águas Residuárias/química , Poluentes Químicos da Água/isolamento & purificação
4.
Mol Nutr Food Res ; 63(21): e1900677, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31483113

RESUMO

Nutritional research is currently entering the field of personalized nutrition, to a large extent driven by major technological breakthroughs in analytical sciences and biocomputing. An efficient launching of the personalized approach depends on the ability of researchers to comprehensively monitor and characterize interindividual variability in the activity of the human gastrointestinal tract. This information is currently not available in such a form. This review therefore aims at identifying and discussing published data, providing evidence on interindividual variability in the processing of the major nutrients, i.e., protein, fat, carbohydrates, vitamins, and minerals, along the gastrointestinal tract, including oral processing, intestinal digestion, and absorption. Although interindividual variability is not a primary endpoint of most studies identified, a significant number of publications provides a wealth of information on this topic for each category of nutrients. This knowledge remains fragmented, however, and understanding the clinical relevance of most of the interindividual responses to food ingestion described in this review remains unclear. In that regard, this review has identified a gap and sets the base for future research addressing the issue of the interindividual variability in the response of the human organism to the ingestion of foods.


Assuntos
Digestão/fisiologia , Trato Gastrointestinal/fisiologia , Aminoácidos/farmacocinética , Variação Biológica Individual , Carboidratos da Dieta/farmacocinética , Gorduras na Dieta/farmacocinética , Proteínas Alimentares/farmacocinética , Microbioma Gastrointestinal , Humanos , Absorção Intestinal , Minerais/farmacocinética , Peptídeo Hidrolases/metabolismo , Polimorfismo Genético , Vitaminas/farmacocinética
5.
J Anim Sci ; 97(2): 794-802, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30418571

RESUMO

Two experiments were conducted to estimate standardized mineral (Ca, P, Cu, Fe, Mn, and Zn) availabilities (SMA) in commonly used feedstuffs and verify the additivity of SMA for diet formulation of broilers. In Exp. 1, after 3 d of acclimation, a total of 96 22-d-old Arbor Acres male broilers were fasted for 24 h. Then the broilers were fed a mineral-free, corn, soybean meal (SBM), or corn-soybean meal (C-SBM) diet for 4 h and the excreta samples were collected for 48 h after feed withdrawal (totally 52 h). The results showed that the endogenous losses of Ca, P, Cu, Fe, Mn, and Zn for chickens fed the mineral-free diet were 13.7, 113, 0.064, 0.593, 0.094, and 0.132 mg/52 h per bird, respectively. The standardized availability values of Ca, P, Cu, Fe, Mn, and Zn were 20.8%, 39.6%, 37.8%, 47.4%, 10.7%, and 45.2% in corn as well as 54.3%, 51.1%, 39.7%, 27.6%, 26.1%, and 51.0% in SBM, respectively. The determined values of SMA in C-SBM diet were close (P > 0.09) to the predicted summations of SMA from corn and SBM (Ca, 48.6% vs. 48.1%; P, 44.9% vs. 46.0%; Cu, 41.7% vs. 39.1%; Fe, 35.3% vs. 33.2%; Mn, 22.7% vs. 22.2%; Zn, 46.2% vs. 48.4%). In Exp. 2, a total of 144 22-d-old Arbor Acres male broilers were used to estimate the SMA values in 6 feedstuffs by using the above procedure. The results showed that the standardized availability values in wheat, wheat bran, corn distillers dried grains with solubles, cottonseed meal, rapeseed meal, and corn gluten meal diets were 24.7% to 55.8% for Ca, 35.6% to 46.6% for P, 24.5% to 45.7% for Cu, 21.6% to 43.5% for Fe, 9.3% to 34.5% for Mn, and 22.9% to 52.9% for Zn, respectively. The results from the present study indicated that the mineral-free diet could be used for estimating the endogenous losses of the above minerals and SMA values of feedstuffs for broilers, and the estimates of SMA in feedstuffs for the C-SBM diet formulation were additive.


Assuntos
Ração Animal/análise , Galinhas , Minerais/farmacocinética , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Masculino , Minerais/administração & dosagem , Glycine max , Zea mays
6.
Chemosphere ; 212: 1076-1084, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30286537

RESUMO

This work aimed to study the role of arbuscular mycorrhizal fungi (AMF) in Hg and major mineral nutrient uptake and tissue localization of these elements in the roots of maize plants. Maize plants were grown in pots filled with non- and Hg-contaminated substrate (50 µg Hg g-1 as HgCl2) and inoculated with two types of AMF inocula: a) Glomus sp. originating from Hg-polluted soil of a former Hg smelting site in Idrija, Slovenia, and b) commercial AM inoculum Symbivit. Controls were inoculated by corresponding bacterial extracts only. Tissue localization of Hg and major mineral nutrients was performed by laser ablation-inductively coupled plasma-mass spectroscopy (LA-ICP-MS) on cryofixed and freeze-dried root cross-sections. AMF colonization increased plant biomass in non-contaminated substrate, while this effect was not seen in Hg-contaminated substrate. Hg increased total plant biomass more than AMF inoculation, possibly through hormetic effects. AMF increased Hg uptake into the roots, as well as Hg transfer to the shoots. AMF affected plant mineral nutrient uptake, depending on the type of AMF inoculum and the presence of Hg. In the roots, Hg was mainly localized in rhizodermis and endodermis, followed by the cortex and the central cylinder. Higher Hg concentrations were detected in the central cylinder of AM plants than in that of the controls, pointing to a higher Hg mobility and potential bioavailability in AMF inoculated plants.


Assuntos
Mercúrio/farmacocinética , Minerais/farmacocinética , Micorrizas/metabolismo , Raízes de Plantas/metabolismo , Zea mays/metabolismo , Biomassa , Raízes de Plantas/química , Eslovênia , Poluentes do Solo/farmacocinética , Distribuição Tecidual , Zea mays/microbiologia
7.
Biomed Res ; 39(4): 215-222, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30101841

RESUMO

Previous studies have shown that dissolved substances in some natural hot springs have analgesic/anti-nociceptive and anti-inflammatory actions. However, the mechanisms underlying how such dissolved substances exert these actions are not fully understood. In the present study on mice, we examined the analgesic/anti-nociceptive and anti-inflammatory properties of a mineral cream containing natural hot spring ingredients. The anti-nociceptive effects of the mineral cream were assessed by using the von Frey test. Application of the mineral cream to the hind paw of mice produced a significant anti-nociceptive effect compared to control. The anti-nociceptive effects of the mineral cream were also assessed following the injection of complete Freund's adjuvant (CFA) into the hind paws of mice after pre-treatment for one or four weeks with the mineral cream. Histological experiments with light microscopy showed that the mineral cream did not reduce inflammation caused by the CFA treatment. In addition, the mineral cream did not inhibit oxidative stress as evidenced by increased levels of oxidative metabolites (d-ROMs) and biological anti-oxidant potential (BAP). These results suggest that the mineral cream does not exert a protective effect against inflammation, and that the constituents of the mineral cream may produce their anti-nociceptive effects transdermally via different mechanisms including the nervous system.


Assuntos
Analgésicos/farmacologia , Balneologia , Minerais/farmacologia , Creme para a Pele/farmacologia , Analgésicos/farmacocinética , Animais , Masculino , Camundongos , Camundongos Endogâmicos ICR , Minerais/farmacocinética , Creme para a Pele/farmacocinética
8.
Mol Nutr Food Res ; 62(21): e1800482, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30095861

RESUMO

SCOPE: The aim of the study is to determine the effects of sheep milk consumption in comparison to cow milk on the mineral distribution in the soft organs and serum in a growing animal model system. As the mineral composition of cow and sheep milk differs, different effects on the bio-accumulations in the body may be observed. Differences in the mineral composition of cow and sheep milk may lead to different bioavailability or accumulation of minerals in the body. Newly weaned rats were fed either cow milk, sheep milk, or sheep milk diluted so that it had the same solid content as cow milk. At the end of the feeding trial, the concentration of minerals in the organs and plasma of the rats was assessed. The results indicate that the consumption of the high level of minerals in sheep milk does not have any negative effects in the rat model. METHODS AND RESULTS: Newly weaned male rats were fed ad libitum for 28 days on either cow milk, sheep milk, or sheep milk diluted to have the same concentration of milk solids as cow milk. Animals were euthanized and the soft organs and serum were harvested and then analyzed for mineral composition by inductively coupled plasma MS. Rats fed sheep milk had lower iron concentrations in the liver and higher concentrations of rubidium and cesium in all of the soft organs. The growth rate of the rats was not affected by the type of milk consumed. CONCLUSION: The concentration of essential and trace minerals in the liver is found to be significantly different between rats fed sheep milk compared to those fed cow milk (p < 0.05). The consumption of sheep milk does not affect the growth of animals.


Assuntos
Leite , Minerais/análise , Oligoelementos/análise , Animais , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Leite/química , Minerais/sangue , Minerais/farmacocinética , Ratos Sprague-Dawley , Ovinos , Distribuição Tecidual , Oligoelementos/sangue , Desmame
9.
Adv Nutr ; 9(4): 524S-532S, 2018 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-30032231

RESUMO

The ability of certain foods to impair or augment the absorption of various vitamins and minerals has been recognized for many years. However, the contribution of botanical dietary supplements (BDSs) to altered micronutrient disposition has received little attention. Almost half of the US population uses some type of dietary supplement on a regular basis, with vitamin and mineral supplements constituting the majority of these products. BDS usage has also risen considerably over the last 2 decades, and a number of clinically relevant herb-drug interactions have been identified during this time. BDSs are formulated as concentrated plant extracts containing a plethora of unique phytochemicals not commonly found in the normal diet. Many of these uncommon phytochemicals can modulate various xenobiotic enzymes and transporters present in both the intestine and liver. Therefore, it is likely that the mechanisms underlying many herb-drug interactions can also affect micronutrient absorption, distribution, metabolism, and excretion. To date, very few prospective studies have attempted to characterize the prevalence and clinical relevance of herb-micronutrient interactions. Current research indicates that certain BDSs can reduce iron, folate, and ascorbate absorption, and others contribute to heavy metal intoxication. Researchers in the field of nutrition may not appreciate many of the idiosyncrasies of BDSs regarding product quality and dosage form performance. Failure to account for these eccentricities can adversely affect the outcome and interpretation of any prospective herb-micronutrient interaction study. This review highlights several clinically relevant herb-micronutrient interactions and describes several common pitfalls that often beset clinical research with BDSs.


Assuntos
Interações Medicamentosas , Micronutrientes/administração & dosagem , Minerais/administração & dosagem , Preparações de Plantas/administração & dosagem , Vitaminas/administração & dosagem , Suplementos Nutricionais , Humanos , Absorção Intestinal/efeitos dos fármacos , Metais/administração & dosagem , Metais/toxicidade , Micronutrientes/farmacocinética , Minerais/farmacocinética , Compostos Fitoquímicos/administração & dosagem , Extratos Vegetais/administração & dosagem , Estudos Prospectivos , Estados Unidos , Vitaminas/farmacocinética
10.
Artigo em Inglês | MEDLINE | ID: mdl-29746791

RESUMO

Concentrations of Al, Ba, Cd, Ca, Co, Cu, Fe, K, Mg, Mn, Hg, Rb, Ag, Na, Sr and Zn were analysed in fruiting bodies of A. muscaria. This mushroom is considered conditionally edible, since parboiling can detoxify its fruiting bodies from the hallucinogens and render it edible. The specific purpose of the research is the little-known phenomenon of the variability of mineral composition of mushrooms for generations harvested in the same forest areas - both in terms of their nutritional value and anthropogenic influences. Fungal materials were digested in nitric acid and analysed by a validated methods using inductively coupled plasma optical emission spectrometer (ICP-OES) and cold - vapor atomic absorption spectroscopy (CV-AAS). A. muscaria collected in the same area over the period of four years showed fluctuations in the concentrations of the essential elements Co, Cu, Fe, K, Mn, Na and Zn (p < 0.05; U Mann-Whitney test), while only Ca and Mg (p > 0.05) levels were similar. In addition, concentrations of Ag, Al, Ba, Cd, Rb and Sr in fruiting bodies fluctuated, while remained at a similar level for Hg. It is important to note that statistically significant variations in levels of several inorganic elements accumulated in A. muscaria would imply a more careful consideration would be required when assessing the nutritional value of mushroom species.


Assuntos
Amanita/química , Amanita/metabolismo , Compostos Inorgânicos/análise , Compostos Inorgânicos/farmacocinética , Agaricales/química , Agaricales/metabolismo , Mercúrio/análise , Mercúrio/farmacocinética , Metais/análise , Metais/farmacocinética , Minerais/análise , Minerais/farmacocinética , Polônia , Espectrofotometria Atômica , Oligoelementos/análise , Oligoelementos/farmacocinética
11.
Artigo em Inglês | MEDLINE | ID: mdl-29652222

RESUMO

Low cost naturally available bone meal was carbonized and its fluoride adsorption capacity was explored. Carbonized bone meal (CBM) produced at 550°C, 4 h carbonization time and a heating rate of 60°C/min, showed fluoride adsorption capacity of 14 mg g-1. Adsorbent was characterized using scanning electron microscopy, X-ray diffraction, X-ray fluoroscence, thermogravimetric analysis and Fourier transform infrared spectroscopy to highlight its physical and chemical properties. Best fluoride uptake capacity was observed for 0.2 mm particle size, 7 g L-1 adsorbent concentration and at pH 6.5. Fluoride uptake was endothermic and chemisorption in nature. Effective diffusivity and mass transfer coefficient were obtained as 6 × 10-11 m2 s-1 and 9 × 10-5 m s-1 from shrinking core model. Sulphate and carbonate showed the highest interference effect on adsorption of fluoride by CBM. Maximum desorption was observed at basic pH (pH 12). Fixed bed study was performed and effect of different parameters (bed height, inlet flow rate and initial concentration) was investigated. Efficiency of the adsorbent using real life fluoride contaminated groundwater solution was also observed.


Assuntos
Carbono/química , Fluoretos/isolamento & purificação , Água Subterrânea , Minerais/química , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , Adsorção , Animais , Técnicas de Cultura Celular por Lotes/instrumentação , Técnicas de Cultura Celular por Lotes/métodos , Produtos Biológicos/química , Produtos Biológicos/farmacocinética , Bovinos , Fluoretos/química , Fluoretos/farmacocinética , Água Subterrânea/análise , Água Subterrânea/química , Concentração de Íons de Hidrogênio , Cinética , Microscopia Eletrônica de Varredura , Minerais/farmacocinética , Fosfatos/química , Espectroscopia de Infravermelho com Transformada de Fourier , Poluentes Químicos da Água/química , Difração de Raios X
12.
Food Res Int ; 105: 233-240, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29433211

RESUMO

Traditional Mediterranean plant-based dishes could allow tackling malnutrition while preserving the cultural heritage. To determine the effect of the cooking method on mineral bioavailability, the content in minerals and chelators of Mloukhiya, a Mediterranean dish based on jute leaves (Corchorus olitorius) that contains also meat, was monitored during the whole cooking process. Mineral bioaccessibility was assessed by measuring in vitro dialyzability. Model equation was also used to estimate mineral bioavailability. Comparison of Mloukhiya samples collected at different cooking time points showed that the dish total mineral content did not change despite the exchanges between sauce and meat during cooking. However, iron bioavailability decreased, because 58% of heme iron was degraded after 5h of cooking and non-heme iron showed poor bioaccessibility (1.2%), mainly due to its high content of phenolic compounds. The bioaccessibility of other minerals (zinc, calcium, magnesium and potassium) was high, indicating that the food matrix had no or little effect. The mineral bioavailability values predicted by using mathematical models were of the same order of magnitude as the bioaccessibility values.


Assuntos
Culinária/métodos , Corchorus/química , Carne/análise , Minerais , Verduras/química , Animais , Disponibilidade Biológica , Bovinos , Dieta Mediterrânea , Minerais/análise , Minerais/química , Minerais/farmacocinética , Folhas de Planta/química
13.
Microsc Res Tech ; 81(2): 181-190, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29083075

RESUMO

This study aimed to test the hypotheses that (i) a parameter related to permeability, αd (ratio of squared water volume by the nonmineral volume) is, among all major component volumes (mineral, water, and organic volumes) the best predictor of quinoline infiltration in natural enamel caries (NEC), and (ii) the pore volume fraction infiltrated by quinoline (Vqui ) in NEC is much lower than previous estimates that neglected water and organic enamel volumes. Mineral and nonmineral volumes and αd were measured at 341 histological points (from 20 approximal NEC lesions), and transport of quinoline was tracked by orientation-independent polarizing microscopy. R2 values of Vqui were 0.596 (αd ), 0.033 (mineral volume), 0.474 (water volume), and 0.011 (organic volume). Vqui values were 23% (body of the lesion), 7% (dark zone), and 9% (translucent zone), lower than previous estimates (with high effect size). Transport of quinoline occurred both parallelly and perpendicularly to prism paths, and dark zones were seen where only transport parallel to prisms occurred. In conclusion, αd was the main predictor of quinoline infiltration, but it differed from the water volume with a small effect size, and the pore volume fraction with quinoline was much lower than previous estimates.


Assuntos
Cárie Dentária/diagnóstico por imagem , Esmalte Dentário/diagnóstico por imagem , Microscopia de Polarização/métodos , Quinolinas/farmacocinética , Dente/diagnóstico por imagem , Humanos , Microrradiografia/métodos , Minerais/farmacocinética , Permeabilidade
14.
Crit Rev Food Sci Nutr ; 58(1): 146-168, 2018 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-26852789

RESUMO

Potatoes (Solanum tuberosum) are an important global crop that can be transformed into many products impacting several health dimensions ranging from undernutrition, food security and disease prevention to issues of overnutrition including obesity, diabetes, heart disease. Processed potato products are typically categorized as high fat and sodium foods, as well as being classified as a significant source of carbohydrate, in the form of starch. Conversely, potato products are less known for their contribution of key micronutrients (vitamin C, potassium, magnesium), fiber, and phytochemicals (phenolics and carotenoids). More recent insight into the nutritional value of potatoes and the potential of potato phytochemicals to modulate oxidative and inflammatory stress as well as the potential to alter glycemic response has resulted in increased interest in strategies to improve and leverage the nutritional quality of processed potatoes. This review summarizes critical information on nutritional profiles of potatoes and their processed products and describes the state of the science relative to the influence of in-home and common commercial processing on nutritional quality and potential impacts on human health.


Assuntos
Manipulação de Alimentos/métodos , Nível de Saúde , Valor Nutritivo , Compostos Fitoquímicos/análise , Tubérculos/química , Solanum tuberosum , Disponibilidade Biológica , Carotenoides/análise , Carboidratos da Dieta/análise , Carboidratos da Dieta/metabolismo , Fibras na Dieta/administração & dosagem , Proteínas Alimentares/análise , Proteínas Alimentares/química , Proteínas Alimentares/metabolismo , Digestão , Humanos , Micronutrientes/administração & dosagem , Minerais/farmacocinética , Fenóis/análise , Compostos Fitoquímicos/farmacocinética , Solanum tuberosum/crescimento & desenvolvimento , Amido/análise , Vitaminas/farmacocinética
15.
Biol Trace Elem Res ; 182(1): 140-146, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28580502

RESUMO

The aim of this study was to measure the content of calcium, magnesium, iron, zinc, and copper and determine the bioavailability of these ingredients in gluten-free breads fortified with milk and selected seeds. Due to the increasing prevalence of celiac disease and mineral deficiencies, it has become necessary to produce food with higher nutritional values which maintains the appropriate product characteristics. This study was designed for gluten-free breads fortified with milk and seeds such as flax, poppy, sunflower seeds, pumpkin seeds or nuts, and flour with amaranth. Subsequently, digestion was performed in vitro and the potential bioavailability of the minerals was measured. In the case of calcium, magnesium, iron, and copper, higher bioavailability was observed in rice bread, and, in the case of copper and zinc, in buckwheat bread. This demonstrated a clear increase in bioavailability of all the minerals when the bread were enriched. However, satisfactory results are obtained only for the individual micronutrients.


Assuntos
Pão/análise , Cálcio/análise , Cobre/análise , Aditivos Alimentares/análise , Ferro/análise , Magnésio/análise , Disponibilidade Biológica , Cálcio/farmacocinética , Cobre/farmacocinética , Dieta Livre de Glúten , Fagopyrum , Farinha/análise , Humanos , Ferro/farmacocinética , Magnésio/farmacocinética , Minerais/análise , Minerais/farmacocinética , Valor Nutritivo , Oryza
16.
Plant Foods Hum Nutr ; 72(4): 335-344, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29098639

RESUMO

This review covers the nutritional significance of tef cereal as compared to other common cereals with emphasis on carbohydrate content and starch digestibility, protein content, iron and zinc bioavailability and antioxidant potentials. Tef is a gluten free cereal and contains the highest iron and calcium among other cereals. It has high micro- and macro- nutritional profile and is becoming globally popular in the healthy grain food chain. Tef starch has a high gelatinization temperature, an essential precondition in the preparation of low glycemic index foods. There are significantly conflicting reports of iron content of tef ranging from 5 to 150 mg/100 g dm. The traditional fermentation of injera reduced majority of the phytic acid but no significant change to mineral bioavailability was observed. This review indicated that studies on starch digestibility, protein characterization, amylase and protease inhibitors, mineral bioavailability and antioxidant potentials are needed to further explore the nutritional and health benefits of tef.


Assuntos
Grão Comestível , Eragrostis , Antioxidantes/análise , Disponibilidade Biológica , Grão Comestível/química , Eragrostis/química , Humanos , Minerais/farmacocinética , Valor Nutritivo , Fenóis/análise , Proteínas de Plantas/análise , Amido/química , Amido/metabolismo
17.
J Periodontal Res ; 52(4): 772-786, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28261803

RESUMO

BACKGROUND AND OBJECTIVE: In regenerative dentistry, platelet preparations are applied to stimulate bone healing and periodontal regeneration. Here, we pursue a strategy where bone substitutes are used as carriers for platelet-released supernatants. The mitogenic capacity and release kinetics of loaded bone substitutes were assessed. MATERIAL AND METHODS: Platelet-released supernatants of washed platelets (washed PRS) and platelet-released supernatants of unwashed platelets (unwashed PRS) were lyophilized onto the bone substitutes deproteinized bovine bone mineral, hydroxyapatite and ß-tricalcium phosphate. Scanning electron microscopy images were taken. Supernatants of bone substitutes were collected at hours 1, 3, 6, 24, and 48 and medium was replaced. We evaluated the protein content with the bicinchoninic acid assay and the effect on proliferation using bioassays with human periodontal fibroblasts. Release of growth factors from the loaded bone substitutes was measured based on the platelet-derived growth factor isoform (PDGF-BB) and thrombin immunoassays. Furthermore, we assessed DNA and RNA content of washed PRS and unwashed PRS. RESULTS: Unwashed PRS showed higher total protein concentrations than washed PRS, while the concentration of PDGF-BB, thrombin, DNA, RNA and their mitogenic effect was not significantly different. The bone substitute materials adsorbed protein over time but no significant changes in overall appearance was found. Supernatants collected from unwashed PRS-loaded bone substitute after 1 h induced a potent mitogenic response in periodontal fibroblasts. This pro-mitogenic capacity of the supernatants decreased over the observation period. Supernatants of washed PRS-loaded bone substitutes did not induce a substantial mitogenic effect. Levels of PDGF-BB, thrombin and protein were higher in supernatants of unwashed PRS-loaded bone substitutes than of washed PRS-loaded bone substitutes. CONCLUSION: Bone substitutes loaded with unwashed PRS, but not bone substitutes loaded with washed PRS show continuously declining release kinetics. These data suggest that plasma components in platelet preparations can modify the release kinetics profile.


Assuntos
Plaquetas/fisiologia , Substitutos Ósseos/farmacocinética , Minerais/farmacocinética , Animais , Fosfatos de Cálcio/farmacocinética , Bovinos , Durapatita/farmacocinética , Fibroblastos/metabolismo , Humanos , Microscopia Eletrônica de Varredura , Fator de Crescimento Derivado de Plaquetas/farmacocinética
18.
Biol Trace Elem Res ; 177(2): 353-366, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27830451

RESUMO

Due to their small size, zinc oxide (ZnO) nanoparticles (NPs) are readily absorbed and easily cross biological barriers, which make them promising candidates as diet additives. However, some studies have reported that ZnO NPs cause toxicity; therefore, their safety and potency as diet additives for farm animals should be established. This study was the first to fully evaluate the effects of ZnO NPs on the homeostasis of eight elements in seven organs/tissues. The regulation of element homeostasis was found to be organ specific with no influence on oxidation status, anti-oxidation capability, or organ damage. ZnO NPs may specifically regulate the homeostasis of mineral elements and affect the following correlations: (1) between the element content in each organ and the concentration of Zn used in ZnSO4 or ZnO NP treatments; (2) between ZnO NP and ZnSO4 treatments for the same element in each organ; and (3) between elements (in each organ in ZnSO4 or ZnO NP treatments) in layers' organs/tissues. The use of ZnO NPs as diet additives for animals should be implemented cautiously because, among other uncertainties, they may affect mineral element content.


Assuntos
Galinhas/metabolismo , Minerais/metabolismo , Nanopartículas/análise , Oligoelementos/metabolismo , Óxido de Zinco/farmacologia , Animais , Homeostase/efeitos dos fármacos , Minerais/análise , Minerais/farmacocinética , Distribuição Tecidual , Oligoelementos/análise , Oligoelementos/farmacocinética , Óxido de Zinco/análise
19.
J Agric Food Chem ; 64(44): 8420-8424, 2016 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-27731991

RESUMO

Interest in the consumption of insects (entomophagy) as an alternative environmentally sustainable source of protein in the diet of humans has recently witnessed a surge. Knowledge of the nutrient composition and, in particular, the bioavailability of minerals from insects is currently sparse. This study evaluated the availability of Fe, Ca, Cu, Mg, Mn, and Zn from four commonly eaten insects and compared these to sirloin beef. Soluble iron from the samples was measured by inductively coupled plasma optical emission spectrometry (ICP-OES). Iron bioavailability was determined using an in vitro simulated peptic-pancreatic digestion, followed by measurement of ferritin (a surrogate marker for iron absorption) in Caco-2 cells. Cricket and sirloin beef had comparably higher levels of Fe, Ca, and Mn than grasshopper, meal, and buffalo worms. However, iron solubility was significantly higher from the insect samples than from beef. The complementation of whole-wheat flour with insect or beef protein resulted in overall decreases in mineral content and iron solubility in the composite mixtures. Collectively, the data show that grasshopper, cricket, and mealworms contain significantly higher chemically available Ca, Cu, Mg, Mn, and Zn than sirloin. However, buffalo worms and sirloin exhibited higher iron bioavailability comparable to that of FeSO4. Commonly consumed insect species could be excellent sources of bioavailable iron and could provide the platform for an alternative strategy for increased mineral intake in the diets of humans.


Assuntos
Insetos/química , Ferro/farmacocinética , Carne Vermelha , Animais , Disponibilidade Biológica , Células CACO-2 , Ferritinas/metabolismo , Gafanhotos/química , Gryllidae/química , Humanos , Ferro/análise , Minerais/análise , Minerais/farmacocinética , Solubilidade , Tenebrio/química , Triticum/química
20.
J Environ Manage ; 183(Pt 3): 521-529, 2016 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-27623366

RESUMO

Recently, heavy metals pollution due to industrialization and urbanization, use of untreated wastewater and unreasonable use of pesticides and fertilizers is increasing rapidly, resulting in major threat to the environment and contaminate soils. Silicon (Si) is the second most abundant element in the earth crust after oxygen. Although it's higher accumulation in plants, yet Si has not been listed as essential nutrient however, considered as beneficial element for growth of plants particularly in stressed environment. Research to date has demonstrated that silicon helps the plants to alleviate the various biotic and abiotic stresses. This review article presents a comprehensive update about Si and heavy metals, minerals and salinity stresses, and contained the progress about Si so far done worldwide in the light of previous studies to evaluate the ecological importance of Si. Moreover, this review will also be helpful to understand the Si uptake ability and its benefits on plants grown under stressed environment. Further research needs for Si-mediated mitigation of heavy metals and mineral nutrients stresses are also discussed.


Assuntos
Metais Pesados/farmacocinética , Plantas/metabolismo , Silício/farmacocinética , Disponibilidade Biológica , Poluição Ambiental , Metais Pesados/toxicidade , Minerais/farmacocinética , Plantas/efeitos dos fármacos , Salinidade , Solo/química , Poluentes do Solo/farmacocinética , Poluentes do Solo/toxicidade , Estresse Fisiológico
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