Assuntos
Neoplasias do Ânus/microbiologia , Carcinoma de Células Escamosas/microbiologia , Papillomaviridae/isolamento & purificação , Neoplasias Penianas/microbiologia , Neoplasias do Colo do Útero/microbiologia , Adulto , Idoso , Neoplasias do Ânus/epidemiologia , Brasil/epidemiologia , Sondas de DNA de HPV , Feminino , Humanos , Hibridização In Situ , Incidência , Masculino , Pessoa de Meia-Idade , Neoplasias Penianas/epidemiologia , Neoplasias do Colo do Útero/epidemiologiaRESUMO
Homologous, subgenomic fragments of the viral LCR and E6/E7 transforming genes of HPV-18 and HPV-16 were amplified from several primary cervical, penile, and vulvar tumors and cloned into a pUC-18-derived vector. When assayed by a quantitative transformation assay using primary human keratinocytes, the subgenomic regions of HPV-16 and HPV-18 exhibited transforming activities similar to that of the full-length, prototype HPV genomes. More importantly, the HPV-18 LCR-E6-E7 region was approximately 10- to 50-fold more active than that of HPV-16. These studies demonstrate (1) that the transforming activity differences previously observed between prototype HPV-16 and HPV-18 map to the LCR-E6-E7 region, and (2) that individual and independent isolates of HPV-16 and HPV-18 exhibit consistent differences in transforming potential, even when isolated from different anatomic sites.
Assuntos
Transformação Celular Neoplásica , Genes Virais , Papillomaviridae/genética , Sequência de Bases , Feminino , Humanos , Masculino , Dados de Sequência Molecular , Papillomaviridae/isolamento & purificação , Neoplasias Penianas/microbiologia , Mapeamento por Restrição , Homologia de Sequência do Ácido Nucleico , Infecções Tumorais por Vírus/microbiologia , Neoplasias do Colo do Útero/microbiologia , Neoplasias Vulvares/microbiologiaRESUMO
To detect the presence of human papillomavirus (HPV) DNA in penile cancers, 18 squamous-cell carcinomas were studied by Southern blot analysis. All specimens were from Brazilian patients ranging in age from 31 to 78 years. Of these, 7 harbored DNA sequence homology. Most positive specimens contained more than 100 copies of viral DNA. No HPV 16 was detected in our samples. Presence of HPV DNA sequences in the samples studied was not correlated with the patients' age or race, nor with extent or progress of disease.
Assuntos
Carcinoma de Células Escamosas/microbiologia , DNA Viral/análise , Papillomaviridae/genética , Neoplasias Penianas/microbiologia , Adulto , Idoso , Brasil , Humanos , Masculino , Pessoa de Meia-Idade , Hibridização de Ácido NucleicoRESUMO
Human papillomavirus (HPV) type-16 DNA sequences were found in 26/53 (49%) and HPV type-18 in 5/53 (9%) of penile cancers. In only one specimen was HPV18 found on its own. HPV16 sequences were integrated into the host cell chromosomes although some monomeric and oligomeric free forms of DNA were detected in a few tissues. HPV18 was, as far as detectable, free and unintegrated in all tissues tested. HPV16 DNA was also detected in 40% of cases of carcinoma of the cervix in 19 women from the same social groups as the males. The viral DNA in the female cases was a mixture of integrated and free forms. The DNA binding protein (ICSP 11/12) of herpes simplex type 2 (HSV2) was not detected in 10 penile cancers tested with a monoclonal antibody.