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1.
Infect Disord Drug Targets ; 24(4): 8-11, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38757698

RESUMO

Background: Ochrobactrum anthropi spp. is a non-enteric, aerobic gram-negative bacillus that has been reported to cause sepsis and occasionally bacteremia in both immunocompetent and immunocompromised hosts. This bacterium is capable of surviving in various habitats, but due to its affinity for aqueous environments, O. anthropi is hypothesized to have an affinity for indwelling plastic devices and other foreign bodies.

Case Presentation: We report a case of a 66 y/o male with a history of polysubstance abuse disorder admitted for toxic metabolic encephalopathy and found to have bronchopneumonia and bacteremia secondary to O. anthropi infection resulting in sepsis and cardiopulmonary arrest.

Discussion: Ochrobactrum spp. is an unusual pathogen of low virulence and has been noted to cause bacteremia and occasionally sepsis in both immunocompetent and immunosuppressed patients. Isolation of this pathogen in the appropriate setting should be considered a true pathogen and treated as such to avoid sequela of this infection.

Conclusion: This case report and literature review suggest that Ochrobactrum anthropi appears more frequently as a pathogen in nosocomial infections than suggested in the literature.

.


Assuntos
Bacteriemia , Infecções por Bactérias Gram-Negativas , Ochrobactrum anthropi , Humanos , Ochrobactrum anthropi/isolamento & purificação , Ochrobactrum anthropi/patogenicidade , Masculino , Bacteriemia/microbiologia , Bacteriemia/tratamento farmacológico , Bacteriemia/complicações , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/complicações , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/diagnóstico , Idoso , Antibacterianos/uso terapêutico , Infecção Hospitalar/microbiologia , Pneumonia/microbiologia
2.
J Invertebr Pathol ; 150: 54-62, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28916147

RESUMO

We report the isolation and identification of seven bacterial strains and one fungal strain from dead and diseased Scapteriscus borellii mole crickets collected from a golf course in southern California. Using 16S and 18S rRNA gene sequence analysis we identified the microbes as Serratia marcescens (red), S. marcescens (white), S. marcescens (purple), Achromobacter xylosoxidans, Chryseobacterium sp., Ochrobactrum anthropi, Tsukamurella tryosinosolvens, and Beauveria bassiana. We performed a dose response curve for each of these cricket-associated microbial strains (except T. tryosinosolvens) and two other strains of S. marcescens (DB1140 and ATCC 13880). We found that all of these microbes except O. anthropi were highly pathogenic to D. melanogaster compared to the other strains of S. marcescens. Injecting the mole cricket associated strains of Serratia into flies killed all infected flies in ≤24h. For all other strains, the median time to death of injected flies varied in a dose-dependent manner. In vivo growth assessments of these microbes suggested that the host immune system was quickly overcome. We used disease tolerance curves to better understand the host-microbe interactions. Further studies are necessary to understand in mechanistic detail the virulence mechanisms of these mole cricket associated microbes and how this association may have influenced the evolution of mole cricket immunity.


Assuntos
Achromobacter denitrificans/patogenicidade , Beauveria/patogenicidade , Chryseobacterium/patogenicidade , Gryllidae/microbiologia , Ochrobactrum anthropi/patogenicidade , Serratia marcescens/patogenicidade , Achromobacter denitrificans/genética , Animais , Beauveria/genética , Chryseobacterium/genética , Drosophila melanogaster , Ochrobactrum anthropi/genética , Serratia marcescens/genética
4.
Proteomics ; 16(23): 3019-3024, 2016 12.
Artigo em Inglês | MEDLINE | ID: mdl-27753207

RESUMO

Ochrobactrum anthropi is a Gram-negative rod belonging to the Brucellaceae family, able to colonize a variety of environments, and actually reported as a human opportunistic pathogen. Despite its low virulence, the bacterium causes a growing number of hospital-acquired infections mainly, but not exclusively, in immunocompromised patients. The aim of this study was to obtain an overview of the global proteome changes occurring in O. anthropi in response to different growth temperatures, in order to achieve a major understanding of the mechanisms by which the bacterium adapts to different habitats and to identify some potential virulence factors. Combined quantitative mass spectrometry-based proteomics and bioinformatics approaches were carried out on two O. anthropi strains grown at temperatures miming soil/plants habitat (25°C) and human host environment (37°C), respectively. Proteomic analysis led to the identification of over 150 differentially expressed proteins in both strains, out of over 1200 total protein identifications. Among them, proteins responsible for heat shock response (DnaK, GrpE), motility (FliC, FlgG, FlgE), and putative virulence factors (TolB) were identified. The study represents the first quantitative proteomic analysis of O. anthropi performed by high-resolution quantitative mass spectrometry.


Assuntos
Proteínas de Bactérias/metabolismo , Ochrobactrum anthropi/metabolismo , Proteínas de Bactérias/análise , Ecossistema , Interações Hospedeiro-Patógeno/fisiologia , Humanos , Ochrobactrum anthropi/patogenicidade , Ochrobactrum anthropi/fisiologia , Temperatura , Fatores de Virulência/metabolismo
5.
Jpn J Infect Dis ; 63(6): 444-6, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21099098

RESUMO

Ochrobactrum anthropi is an emerging pathogen in immunocompromised patients, with the majority of human cases being central venous catheter-related infections. In contrast, O. anthropi-related biliary sepsis is much rare. Herein we report the clinical and microbiological characteristics of O. anthropi-related biliary sepsis in order to increase awareness of the potential role of O. anthropi in this infection. Further extensive epidemiologic studies should be carried out to ascertain the etiologic association between O. anthropi and biliary sepsis and to identify potential hosts and routes of transmission.


Assuntos
Doenças dos Ductos Biliares/microbiologia , Infecções por Bactérias Gram-Negativas/complicações , Ochrobactrum anthropi/isolamento & purificação , Sepse/microbiologia , Idoso , Antibacterianos/farmacologia , Bacteriemia/microbiologia , Bacteriemia/fisiopatologia , Doenças dos Ductos Biliares/fisiopatologia , Infecções Relacionadas a Cateter/microbiologia , Infecções Relacionadas a Cateter/fisiopatologia , Feminino , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/fisiopatologia , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Ochrobactrum anthropi/efeitos dos fármacos , Ochrobactrum anthropi/patogenicidade , Sepse/fisiopatologia
7.
Mikrobiyol Bul ; 41(3): 469-72, 2007 Jul.
Artigo em Turco | MEDLINE | ID: mdl-17933261

RESUMO

Ochrobactrum anthropi (formerly Achromobacter spp.) is an aerobic, motile, oxidase positive and lactose negative gram negative bacillus which is widely distributed in the environment and water sources. In recent publications, O. anthropi has an increasing importance as a nosocomial infection agent. The aim of this report was to present a case of O. anthropi bacteremia developed after endoscopic retrograde cholangiopancreatography (ERCP). A 89-year old female patient presented with high fever one day after ERCP performed due to klatskin tumour. O. anthropi had been grown in blood culture (BacT/ALERT 3D, bioMérieux, Durham, USA), and the isolate was identified by automatized system (VITEK, bioMerieux, Marcy l'Etoile, France). Since there was no clinical response to empirical ceftriaxone therapy, it was switched to meropenem, which was found effective by VITEK antibiotic susceptibility detection system. The patient was treated successfully with meropenem therapy (3 x 1 gr/day, 10 days). As a result, in case of suspected post-ERCP bacteremia, unconventional microorganisms such as O. anthropi should be taken into consideration.


Assuntos
Colangiopancreatografia Retrógrada Endoscópica/efeitos adversos , Infecção Hospitalar/etiologia , Infecções por Bactérias Gram-Negativas/etiologia , Ochrobactrum anthropi/isolamento & purificação , Idoso de 80 Anos ou mais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Bacteriemia/microbiologia , Infecção Hospitalar/tratamento farmacológico , Infecção Hospitalar/microbiologia , Feminino , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/microbiologia , Humanos , Meropeném , Testes de Sensibilidade Microbiana , Ochrobactrum anthropi/efeitos dos fármacos , Ochrobactrum anthropi/patogenicidade , Tienamicinas/farmacologia , Tienamicinas/uso terapêutico
8.
J Proteome Res ; 5(11): 3145-53, 2006 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17081066

RESUMO

We report the first large-scale gel-free proteomic analysis of the soluble subproteome of the emerging pathogen Ochrobactrum anthropi. Utilizing our robust offline multidimensional protein identification protocol, a total of 57 280 peptides were initially identified utilizing automated MS/MS analysis software. We describe our investigation of the heuristic protein validation tool PROVALT and demonstrate its ability to increase the speed and accuracy of the curation process of large-scale proteomic datasets. PROVALT reduced our peptide list to 8517 identified peptides and further manual curation of these peptides led to a final list of 984 uniquely identified peptides that resulted in the positive identification of 249 proteins. These identified proteins were functionally classified and physiochemically characterized. A variety of typical "housekeeping" functions identified within the proteome included nucleic acid, amino and fatty acid anabolism and catabolism, glycolysis, TCA cycle, and pyruvate and selenoamino acid metabolism. In addition, a number of potential virulence factors of relevance to both plant and human disease were identified.


Assuntos
Proteínas de Bactérias/química , Ochrobactrum anthropi/química , Proteoma , Sequência de Aminoácidos , Automação , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Eletroforese em Gel Bidimensional/métodos , Espectrometria de Massas , Dados de Sequência Molecular , Ochrobactrum anthropi/genética , Ochrobactrum anthropi/patogenicidade , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/isolamento & purificação , Proteômica/métodos
9.
J Clin Microbiol ; 44(3): 1184-6, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16517927

RESUMO

Ochrobactrum intermedium [corrected] infection is rare in humans and is generally associated with immunocompromised hosts with indwelling foreign bodies. We report a case of pelvic abscess with O. intermedium [corrected] after a routine appendectomy in an immunocompetent patient and review the literature on O. intermedium [corrected] infection in patients with normal immune function.


Assuntos
Abscesso/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Ochrobactrum anthropi/patogenicidade , Infecção Pélvica/microbiologia , Abscesso/imunologia , Apendicectomia/efeitos adversos , Infecções por Bactérias Gram-Negativas/imunologia , Humanos , Imunocompetência , Masculino , Pessoa de Meia-Idade , Infecção Pélvica/imunologia , Complicações Pós-Operatórias/imunologia , Complicações Pós-Operatórias/microbiologia
12.
Pediatr Infect Dis J ; 21(1): 72-4, 2002 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11791106

RESUMO

Ochrobactrum anthropi is an emerging pathogen in immunocompromised hosts, particularly in patients with indwelling catheters. We report the characteristics of 14 O. anthropi bacteremic episodes in 11 children with Hickman-type central catheters. Children presented with fever and nonspecific clinical manifestations. Bacteremia was successfully treated with antibiotics, but catheter removal was necessary to achieve cure in four cases.


Assuntos
Bacteriemia/etiologia , Bacteriemia/microbiologia , Infecções por Bactérias Gram-Negativas/etiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Ochrobactrum anthropi/patogenicidade , Antibacterianos/uso terapêutico , Bacteriemia/tratamento farmacológico , Cateterismo Venoso Central/efeitos adversos , Criança , Pré-Escolar , Feminino , Febre/etiologia , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , Lactente , Masculino , Resultado do Tratamento
13.
Scand J Infect Dis ; 34(11): 800-3, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12578145

RESUMO

Ochrobactrum anthropi is an emerging pathogen in immunocompromised patients but infections with the bacterium have very rarely been documented in normal hosts. We report the characteristics of O. anthropi bacteraemia in 11 immunocompetent children, aged 2 months to 7 y, hospitalized in a general hospital during a 5-y period. Children commonly presented with fever, non-specific respiratory or gastrointestinal manifestations, leukocytosis and neutrophilia and had a rapid recovery, even when they did not receive a specific treatment. In 10 cases positive blood cultures were obtained on admission and in all cases subsequent cultures were sterile. In conclusion, O. anthropi may cause bacteraemia in immunocompetent hosts, although further studies are required to clarify whether these isolates represent pseudobacteraemia or whether O. anthropi is a potential pathogen of low virulence.


Assuntos
Bacteriemia/epidemiologia , Bacteriemia/microbiologia , Infecções por Bactérias Gram-Negativas/epidemiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Imunocompetência , Ochrobactrum anthropi/isolamento & purificação , Antibacterianos/uso terapêutico , Bacteriemia/tratamento farmacológico , Bacteriemia/fisiopatologia , Criança , Pré-Escolar , Farmacorresistência Bacteriana Múltipla , Feminino , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/fisiopatologia , Humanos , Lactente , Masculino , Ochrobactrum anthropi/patogenicidade
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