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1.
Mol Ecol Resour ; 16(1): 17-28, 2016 01.
Artigo em Inglês | MEDLINE | ID: mdl-25712438

RESUMO

Gene sequence similarity due to shared ancestry after a duplication event, that is paralogy, complicates the assessment of genetic variation, as sequences originating from paralogs can be difficult to distinguish. These confounded sequences are often removed prior to further analyses, leaving the underlying loci uncharacterized. Salmonids have only partially rediploidized subsequent to a whole-genome duplication; residual tetrasomic inheritance has been observed in males. We present a maximum-likelihood-based method to resolve confounded paralogous loci by observing the segregation of alleles in gynogenetic haploid offspring and demonstrate its effectiveness by constructing two linkage maps for chum salmon (Oncorhynchus keta), with and without these newly resolved loci. We find that the resolved paralogous loci are not randomly distributed across the genome. A majority are clustered in expanded subtelomeric regions of 14 linkage groups, suggesting a significant fraction of the chum salmon genome may be missed by the exclusion of paralogous loci. Transposable elements have been proposed as drivers of genome evolution and, in salmonids, may have an important role in the rediploidization process by driving differentiation between homeologous chromosomes. Consistent with that hypothesis, we find a reduced fraction of transposable element annotations among paralogous loci, and these loci predominately occur in the genomic regions that lag in the rediploidization process.


Assuntos
Oncorhynchus keta/genética , Tetrassomia , Animais , Mapeamento Cromossômico , Cromossomos/genética , Feminino , Variação Genética , Genoma , Masculino , Oncorhynchus keta/classificação
2.
PLoS One ; 8(12): e81916, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24349150

RESUMO

The extent to which stray, hatchery-reared salmon affect wild populations is much debated. Although experiments show that artificial breeding and culture influence the genetics of hatchery salmon, little is known about the interaction between hatchery and wild salmon in a natural setting. Here, we estimated historical and contemporary genetic population structures of chum salmon (Oncorhynchus keta) in Prince William Sound (PWS), Alaska, with 135 single nucleotide polymorphism (SNP) markers. Historical population structure was inferred from the analysis of DNA from fish scales, which had been archived since the late 1960's for several populations in PWS. Parallel analyses with microsatellites and a test based on Hardy-Weinberg proportions showed that about 50% of the fish-scale DNA was cross-contaminated with DNA from other fish. These samples were removed from the analysis. We used a novel application of the classical source-sink model to compare SNP allele frequencies in these archived fish-scales (1964-1982) with frequencies in contemporary samples (2008-2010) and found a temporal shift toward hatchery allele frequencies in some wild populations. Other populations showed markedly less introgression, despite moderate amounts of hatchery straying. The extent of introgression may reflect similarities in spawning time and life-history traits between hatchery and wild fish, or the degree that hybrids return to a natal spawning area. The source-sink model is a powerful means of detecting low levels of introgression over several generations.


Assuntos
Quimera/genética , Fluxo Gênico , Oncorhynchus keta/genética , Salmão/genética , Alaska , Animais , Cruzamento , Conservação dos Recursos Naturais , Feminino , Pesqueiros , Variação Genética , Genética Populacional , Genótipo , Masculino , Repetições de Microssatélites , Modelos Genéticos , Oncorhynchus keta/classificação , Fenótipo , Filogenia
4.
Genetika ; 47(11): 1473-80, 2011 Nov.
Artigo em Russo | MEDLINE | ID: mdl-22332405

RESUMO

A set often microsatellite loci enabling fairly accurate identification of the chum salmon individuals from geographically distant groups was designed at the Laboratory of Genetic Identification, Vavilov Institute of General Genetics, Russian Academy of Sciences. However, identification of the individuals from closely located basins performed using these loci was not sufficiently precise. The present study was focused on the improvement of the resolution of the method through increasing the number microsatellite loci used. In this study, typing of additional microsatellite loci of chum salmon and evaluation of the change of the degree of identification with the increase of the number ofmicrosatellite loci used is described. It was shown that the identification accuracy permanently increased with the increase of the number of microsatellite markers used.


Assuntos
Repetições de Microssatélites/genética , Oncorhynchus keta/classificação , Oncorhynchus keta/genética , Animais , Ásia Oriental , Marcadores Genéticos , Reação em Cadeia da Polimerase/métodos , Federação Russa
5.
Genetika ; 47(11): 1481-90, 2011 Nov.
Artigo em Russo | MEDLINE | ID: mdl-22332406

RESUMO

The nucleotide sequences of a fragment of the mitochondrial DNA cytochrome b gene were determined in 12 chum salmon populations from the Russian Far East. The level of genetic diversity in the chum salmon populations from the Iturup Island, northern coast of the Sea of Okhotsk, and Anadyr' River was found to be higher than in the populations from Kamchatka and Sakhalin, which may be related to the history of their origin and dispersal. The proportions of intrapopulation genetic variability (F(ct)) and interpopulation genetic variability within the groups (F(sc)) account for 90.87 and 0.9%, respectively, and the intergroup component (F(st)) comprises 8.23%. The predominance of one haplotype, B1, which is common for all populations studied, and a low share of intergroup variability suggest the beginning of colonization by the species of the given region from a common source (group of founders) and a relatively recent time of divergence of the chum salmon populations from the region examined.


Assuntos
Citocromos b/genética , DNA Mitocondrial/genética , Oncorhynchus keta/classificação , Oncorhynchus keta/genética , População/genética , Animais , Sequência de Bases , Variação Genética , Haplótipos , Dados de Sequência Molecular , Linhagem , Filogeografia , Reação em Cadeia da Polimerase/métodos , Federação Russa
6.
Mar Biotechnol (NY) ; 9(2): 179-91, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17186428

RESUMO

A newly developed DNA microarray was applied to identify mitochondrial (mt) DNA haplotypes of more than 2200 chum salmon in the Bering Sea and North Pacific Ocean in September 2002 and also 2003, when the majority of maturing fish were migrating toward their natal river. The distribution of haplotypes occurring in Asian and North American fish in the surveyed area was similar in the 2 years. A conditional maximum likelihood method for estimation of stock compositions indicated that the Japanese stocks were distributed mainly in the north central Bering Sea, whereas the Russian stocks were mainly in the western Bering Sea. The North American stocks were abundant in the North Pacific Ocean around the Aleutian Islands. These results indicate that the Asian and North American stocks of chum salmon are nonrandomly distributed in the Bering Sea and the North Pacific Ocean, and further the oligonuleotide DNA microarray developed by us has a high potential for identification of stocks among mixed ocean aggregates of high-seas chum salmon.


Assuntos
DNA Mitocondrial/genética , Variação Genética , Análise de Sequência com Séries de Oligonucleotídeos/veterinária , Oncorhynchus keta/genética , Animais , Primers do DNA/química , Demografia , Genética Populacional , Geografia , Haplótipos , Funções Verossimilhança , Oncorhynchus keta/classificação , Oceano Pacífico , Reação em Cadeia da Polimerase/veterinária
7.
J Agric Food Chem ; 48(6): 2184-8, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10888519

RESUMO

Identification of 10 salmon species using DNA-based methodology was investigated. Amplification of DNA was carried out using a primer set which amplified a region of the mitochondrial cytochrome b gene. Sequences of PCR-amplified DNA from the salmon species were used to select six restriction enzymes allowing species to be uniquely classified. RFLP patterns generated following analysis with each enzyme were resolved using polyacrylamide gel electrophoresis and visualized by silver staining. Results indicate that it is possible to differentiate between all 10 salmon species and that the technique could be easily adopted by the food industry for analysis of processed salmon products.


Assuntos
Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Salmonidae/classificação , Animais , Sequência de Bases , Dados de Sequência Molecular , Oncorhynchus/classificação , Oncorhynchus/genética , Oncorhynchus keta/classificação , Oncorhynchus keta/genética , Oncorhynchus kisutch/classificação , Oncorhynchus kisutch/genética , Oncorhynchus mykiss/classificação , Oncorhynchus mykiss/genética , Mapeamento por Restrição , Salmão/classificação , Salmão/genética , Salmonidae/genética , Alinhamento de Sequência , Análise de Sequência de DNA/métodos , Homologia de Sequência do Ácido Nucleico , Truta/classificação , Truta/genética
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