RESUMO
Pantoea agglomerans inhabit diverse ecological niches, ranging from epiphytes and endophytes in plants, body of animals, and occasionally in the human system. This multifaceted bacterium contributes substantially to plant growth promotion, stress resilience, and biocontrol but can also act as a pathogen to its host. The genetic determinants underlying these diverse functions remain largely unfathomed and to uncover this phenomenon, nineteen strains of Pantoea agglomerans were selected and analyzed. Genome-to-Genome Distance Calculator (GGDC) which uses the Genome Blast Distance Phylogeny (GBDP) technique to calculate digital DDH values. Phylogenetic analysis via Genome-to-Genome distance, Average Nucleotide Identity, and Amino Acid Identity calculation revealed that all strains belonged to the genus Pantoea. However, strain 33.1 had a lower value than the threshold for the same species delineation. Bacterial Pan Genome Analysis (BPGA) Pipeline and MinPath analysis revealed genetic traits associated with environmental resilience, such as oxidative stress, UV radiation, temperature extremes, and metabolism of distinct host-specific carbohydrates. Protein-protein interactome analysis illustrated osmotic stress proteins closely linked with core proteins, while heavy metal tolerance, nitrogen metabolism, and Type III and VI secretion systems proteins generally associated with pathogenicity formed a separate network, indicating strain-specific characteristics. These findings shed new light on the intricate genetic architecture of Pantoea agglomerans, revealing its adaptability to inhabit diverse niches and thrive in varied environments.
Assuntos
Genoma Bacteriano , Pantoea , Filogenia , Pantoea/genética , Pantoea/fisiologia , Pantoea/classificação , Genômica , Adaptação Fisiológica , Humanos , Animais , Plantas/microbiologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismoRESUMO
Changes in the plant microbiota composition are intimately associated with the health of the plant, but factors controlling the microbial community in flowers are poorly understood. In this study, we used apple flowers and fire blight as a model system to investigate the effects of floral microbiota and microbial competition on disease development and suppression. To compare changes in microbial flora with the RNA expression patterns of plants, the flower samples were collected in three different flowering stages (Bud, Popcorn, and Full-bloom). Using advanced sequencing technology, we analyzed the data and conducted both in vitro and in vivo experiments to validate our findings. Our results show that the Erwinia amylovora use arabinogalactan, which is secreted on the flowers, for early colonization of apple flowers. Pantoea agglomerans was more competitive for arabinogalactan than E. amylovora. Additionally, P. agglomerans suppressed the expression of virulence factors of E. amylovora by using arabinose, which is a major component of arabinogalactan, which induces virulence gene expression. The present data provide new insights into developing control strategies for diverse plant diseases, including fire blight, by highlighting the importance of nutrients in disease development or suppression.
Assuntos
Erwinia amylovora , Flores , Galactanos , Malus , Microbiota , Doenças das Plantas , Malus/microbiologia , Erwinia amylovora/patogenicidade , Erwinia amylovora/fisiologia , Doenças das Plantas/microbiologia , Flores/microbiologia , Galactanos/metabolismo , Nutrientes/metabolismo , Pantoea/fisiologia , Pantoea/genética , Pantoea/patogenicidade , Arabinose/metabolismo , Fatores de Virulência/genéticaRESUMO
Leaf microbiota have been extensively applied in the biological control of plant diseases, but their crucial roles in mitigating atmospheric heavy metal (HM) deposition and promoting plant growth remain poorly understood. This study demonstrates that elevated atmospheric HM deposition on rice leaves significantly shapes distinct epiphytic and endophytic microbiota across all growth stages. HM stress consistently leads to the dominance of epiphytic Pantoea and endophytic Microbacterium in rice leaves, particularly during the booting and filling stages. Leaf-bound HMs stimulate the differentiation of specialized microbial communities in both endophytic and epiphytic compartments, thereby regulating leaf microbial interactions. Metagenomic binning retrieved high-quality genomes of keystone leaf microorganisms, indicating their potential for essential metabolic functions. Notably, Pantoea and Microbacterium show significant HM resistance, plant growth-promoting capabilities, and diverse element cycling functions. They possess genes associated with metal(loid) resistance, such as ars and czc, suggesting their ability to detoxify arsenic(As) and cadmium(Cd). They also support carbon, nitrogen, and sulfur cycling, with genes linked to carbon fixation, nitrogen fixation, and sulfur reduction. Additionally, these bacteria may enhance plant stress resistance and growth by producing antioxidants, phytohormones, and other beneficial compounds, potentially improving HM stress tolerance and nutrient availability in rice plants. This study shows that atmospheric HMs affect rice leaf microbial communities, prompting plants to seek microbial help to combat stress. The unique composition and metabolic potential of rice leaf microbiota offer a novel perspective for mitigating adverse stress induced by atmospheric HM deposition. This contributes to the utilization of leaf microbiota to alleviate the negative impact of heavy metal deposition on rice development and food security.
Assuntos
Metais Pesados , Microbiota , Oryza , Folhas de Planta , Oryza/microbiologia , Metais Pesados/metabolismo , Folhas de Planta/metabolismo , Microbiota/efeitos dos fármacos , Estresse Fisiológico , Poluentes Atmosféricos/toxicidade , Pantoea/fisiologiaRESUMO
Here, we demonstrate the beneficial effect of surfactant-producing pseudomonads on Pantoea eucalypti 299R. We conducted a series of experiments in environments of increasing complexity. P. eucalypti 299R (Pe299R), and Pseudomonas sp. FF1 (Pff1) or Pe299R and surfactant-production deficient Pseudomonas sp. FF1::ΔviscB (Pff1ΔviscB) were co-inoculated in broth, on swarming agar plates, and on plants. In broth, there were no differences in the growth dynamics of Pe299R when growing in the presence of Pff1 or Pff1ΔviscB. By contrast, on swarming agar plates, Pe299R was able to co-swarm with Pff1 which led to a significant increase in Pe299R biomass compared to Pe299R growing with Pff1ΔviscB or in monoculture. Finally in planta, and using the single-cell bioreporter for reproductive success (CUSPER), we found a temporally distinct beneficial effect of Pff1 on co-inoculated Pe299R subpopulations that did not occur in the presence of Pff1ΔviscB. We tested three additional surfactant-producing pseudomonads and their respective surfactant knockout mutants on PE299R on swarming agar showing similar results. This led us to propose a model for the positive effect of surfactant production during leaf colonization. Our results indicate that co-motility might be common during leaf colonization and adds yet another facet to the already manyfold roles of surfactants.
Assuntos
Pantoea , Pseudomonas , Tensoativos , Pantoea/genética , Pantoea/metabolismo , Pantoea/fisiologia , Pantoea/crescimento & desenvolvimento , Pseudomonas/metabolismo , Pseudomonas/genética , Pseudomonas/crescimento & desenvolvimento , Pseudomonas/fisiologia , Tensoativos/metabolismoRESUMO
Pantoea dispersa is a Gram-negative bacterium that exists in a variety of environments and has potential in many commercial and agricultural applications, such as biotechnology, environmental protection, soil bioremediation, and plant growth stimulation. However, P. dispersa is also a harmful pathogen to both humans and plants. This "double-edged sword" phenomenon is not uncommon in nature. To ensure survival, microorganisms respond to both environmental and biological stimuli, which could be beneficial or detrimental to other species. Therefore, to harness the full potential of P. dispersa, while minimizing potential harm, it is imperative to unravel its genetic makeup, understand its ecological interactions and underlying mechanisms. This review aims to provide a comprehensive and up-to-date overview of the genetic and biological characteristics of P. dispersa, in addition to potential impacts on plants and humans, as well as to provide insights into potential applications.
Assuntos
Pantoea , Humanos , Biodegradação Ambiental , Pantoea/fisiologia , Solo , Agricultura , PlantasRESUMO
Bdellovibrios are predatory bacteria that invade other live Gram-negative bacterial cells for growth and reproduction. They have recently been considered as potential living antibiotics and biocontrol agents. In this study, the predatory activity and biocontrol potency of Bdellovibrio bacteriovorus strain SOIR-1 against Pantoea sp. strain BCCS and Xanthomonas campestris, two exo-biopolymer-producing phytopathogens, was evaluated. Plaque formation assays and lysis analysis in the broth co-cultures were used for the in vitro evaluation of bacteriolytic activity of strain SOIR-1. The in vivo biocontrol potential of strain SOIR-1 was evaluated by pathogenicity tests on the onion bulbs and potato tuber slices. The phytopathogens were also recovered from the infected plant tissues and confirmed using biochemical tests and PCR-based 16S rRNA gene sequence analysis. Typical bdellovibrios plaques were developed on the lawn cultures of Pantoea sp. BCCS and X. campestris. The killing rate of strain SOIR-1 toward Pantoea sp. BCCS and X. campestris was 84.3% and 76.3%, respectively. Exo-biopolymers attenuated the predation efficiency of strain SOIR-1 up to 10.2-18.2% (Pantoea sp. BCCS) and 12.2-17.3% (X. campestris). The strain SOIR-1 significantly reduced rotting symptoms in the onion bulbs caused by Pantoea sp. BCCS (69.0%) and potato tuber slices caused by X. campestris (73.1%). Although more field assessments are necessary, strain SOIR-1 has the preliminary potential as a biocontrol agent against phytopathogenic Pantoea sp. BCCS and X. campestris, especially in postharvest storage. Due to the particular physicochemical properties of evaluated exo-biopolymers, they can be used in the designing encapsulation systems for delivery of bdellovibrios.
Assuntos
Bdellovibrio bacteriovorus/fisiologia , Bdellovibrio bacteriovorus/patogenicidade , Agentes de Controle Biológico/farmacologia , Pantoea/efeitos dos fármacos , Pantoea/fisiologia , Xanthomonas campestris/efeitos dos fármacos , Xanthomonas campestris/fisiologia , Antibiose , Biopolímeros/fisiologia , Técnicas de Cocultura/métodos , DNA Bacteriano , Interações Microbianas , RNA Ribossômico 16SRESUMO
Pantoea agglomerans YS19 is a dominant endophytic bacterium isolated from rice, which is capable of promoting host plant growth by nitrogen-fixing and phytohormone secreting. We previously found that the cytidine repressor (CytR) protein conducts the regulation of indole signal in YS19. Here, we compared the whole-cell protein of the wild type YS19 and the ΔcytR mutant and subsequently identified one differential protein as alkyl hydroperoxide reductase subunit C related to oxidative stress and sulfur starvation tolerance. It was tested that cytR had a positive effect on the survival of YS19 under the oxidative stress and sulfur starvation conditions and this effect was inhibited by indole. To further understand the functional mode of indole in this regulation, we cloned the cytR promoter region (PcytR) of YS19 and tested the effect of indole on PcytR using gfp as a reporter gene. It was found that PcytR can sense indole and significantly inhibit the expression of the downstream gene. This study provided a deeper understanding of the multiple function of cytR and expanded a new research direction of how indole participates in gene regulation.
Assuntos
Proteínas de Bactérias/metabolismo , Estresse Oxidativo/fisiologia , Pantoea/fisiologia , Proteínas Repressoras/metabolismo , Enxofre/metabolismo , Proteínas de Bactérias/genética , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Indóis/farmacologia , Viabilidade Microbiana/efeitos dos fármacos , Mutação , Estresse Oxidativo/efeitos dos fármacos , Peroxirredoxinas/genética , Regiões Promotoras Genéticas , Proteínas Repressoras/genéticaRESUMO
Phytophagous stink bugs typically harbor nutritional symbiotic bacteria in their midgut, to integrate their unbalanced diet. In the Pentatomidae, most symbionts are affiliated to the genus Pantoea, and are polyphyletic. This suggests a scenario of an ancestral establishment of symbiosis, followed by multiple symbiont replacement events by akin environmental bacteria in different host lineages. In this study, a novel Pantoeaspecies ('CandidatusPantoea persica') was characterized from the gut of the pentatomid Acrosternum arabicum, and shown to be highly abundant in a specific portion of the gut and necessary for the host development. The genome of the symbiont (2.9 Mb), while presenting putative host-supportive metabolic pathways, including those for amino acids and vitamin synthesis, showed a high level of pseudogenization, indicating ongoing genome reduction. Comparative analyses with other free-living and symbiotic Pantoea highlighted a convergent pattern of genome reduction in symbionts of pentatomids, putatively following the typical phases modelized in obligate nutritional symbionts of insects. Additionally, this system has distinctive traits, as hosts are closely related, and symbionts originated multiple independent times from closely related free-living bacteria, displaying convergent and independent conspicuous genome reduction. Due to such peculiarities, this may become an ideal model to study genome evolutionary processes in insect symbionts.
Assuntos
Genoma Bacteriano , Heterópteros/microbiologia , Pantoea/genética , Simbiose , Animais , Evolução Molecular , Heterópteros/fisiologia , Redes e Vias Metabólicas , Pantoea/classificação , Pantoea/isolamento & purificação , Pantoea/fisiologia , FilogeniaRESUMO
The brown marmorated stink bug Halyomorpha halys (Stål) is a globally invasive species that harbours the primary bacterial symbiont 'Candidatus Pantoea carbekii'. In this work, P. carbekii was used as another genetic marker to investigate the biodiversity and biogeographical patterns of this important pest, in native and newly invaded areas, especially in Italy. The correlation between the genetic structure of the symbiont and that of its host was studied through the analyses of one bacterial and one host marker, the putative pseudogene ΔybgF and the mitochondrial gene COI, respectively. As a result, five new P. carbekii haplotypes were identified, and an association pattern between host-symbiont haplotypes was observed. Host species showed higher haplotype diversity than symbiont, which can be expected in a long term host-symbiont association. Populations from the north-eastern Italy showed the highest values of genetic diversity for both markers, highlighting that this particular Italian area could be the result of multiple ongoing introductions. Moreover, some of the symbiont-host haplotypes observed were shared only by populations from north-eastern Italy and native areas, especially Japan, suggesting further introductions from this native country to Italy. Overall, our findings improve the understanding of the potential origin of multiple accidental introductions of H. halys in Italy.
Assuntos
Haplótipos , Heterópteros/genética , Heterópteros/microbiologia , Espécies Introduzidas , Simbiose/fisiologia , Animais , Itália , Pantoea/fisiologiaRESUMO
The Gram-negative Pantoea eucrina D2 was isolated from the marine sponge Chondrosia reniformis. Sponges were collected in a shallow volcanic vents system in Ischia island (South Italy), influenced by CO2 emissions and lowered pH. The chemical diversity of the secondary metabolites produced by this strain, under different culture conditions, was explored by a combined approach including molecular networking, pure compound isolation and NMR spectroscopy. The metabolome of Pantoea cf. eucrina D2 yielded a very complex molecular network, allowing the annotation of several metabolites, among them two biosurfactant clusters: lipoamino acids and surfactins. The production of each class of metabolites was highly dependent on the culture conditions, in particular, the production of unusual surfactins derivatives was reported for the first time from this genus; interestingly the production of these metabolites only arises by utilizing inorganic nitrogen as a sole nitrogen source. Major components of the extract obtained under standard medium culture conditions were isolated and identified as N-lipoamino acids by a combination of 1D and 2D NMR spectroscopy and HRESI-MS analysis. Assessment of the antimicrobial activity of the pure compounds towards some human pathogens, indicated a moderate activity of leucine containing N-lipoamino acids towards Staphylococcus aureus, Staphylococcus epidermidis and a clinical isolate of the emerging food pathogen Listeria monocytogenes.
Assuntos
Antibacterianos/farmacologia , Meios de Cultura/farmacologia , Redes e Vias Metabólicas , Metaboloma/efeitos dos fármacos , Pantoea/fisiologia , Poríferos/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Ácidos/química , Animais , Antibacterianos/química , Antibacterianos/isolamento & purificação , Meios de Cultura/química , Humanos , Filogenia , Poríferos/fisiologiaRESUMO
The deferred antagonism technique has been utilized for several decades for detecting antibiosis activity. Most protocols require the elimination of antibiotic-producing cells by exposing them to chloroform vapour, UV radiation or filter sterilizing the filtrate steps that require additional time and expense to complete. We provide a modified approach to current soft agar overlay practices, which involves addition of antibiotics to the soft agar overlay to inhibit growth of the producer but not the indicator strain. This technique can be used to reproducibly and efficiently screen for antibiotic production with ease. We demonstrate the effectiveness of this technique with three bacterial systems: inhibition of the bacterial spot of tomato pathogen, Xanthomonas euvesicatoria, by its pathogenic competitor Xanthomonas perforans; and inhibition of the fire blight pathogen, Erwinia amylovora, by Pantoea vagans C9-1 or Pseudomonas fluorescens A506. SIGNIFICANCE AND IMPACT OF THE STUDY: Deferred antagonism assays are used commonly to observe antibiotic production by micro-organisms. Killing or removing the producer cells prior to introduction of the indicator strain is a standard practice but requires additional time and special handling procedures. We evaluated a modification of the assay, where the overlay medium is amended with an antibiotic to which the indicator strain is resistant and the producer strain is sensitive. This modification obviates extra steps to kill the producer strain prior to overlaying with the indicator strain and provides a rapid, consistent and cost-effective method to detect antibiosis.
Assuntos
Antibiose , Erwinia amylovora/fisiologia , Técnicas Microbiológicas/métodos , Pantoea/fisiologia , Pseudomonas fluorescens/fisiologia , Xanthomonas/fisiologia , Solanum lycopersicum/microbiologia , Doenças das Plantas/microbiologia , Xanthomonas/crescimento & desenvolvimentoRESUMO
AIMS: Pinus massoniana is one of the most widely distributed forest plants in China. In this study, we isolated a bacterial endophyte (designated FBS135) from apical buds and needles of P. massoniana. Investigations were performed to understand the effects of the strain on pine growth, its genomic features and the functions of the plasmids it carries. METHODS AND RESULTS: Based on its morphological features and 16S rRNA sequence, strain FBS135 was primarily identified as Pantoea eucalypti. We found that FBS135 not only promoted the growth of P. massoniana seedlings, but also significantly increased the survival rate of pine seedlings. The whole genome of FBS135 was sequenced, which revealed that the bacterium carries one chromosome and four plasmids. Its chromosome is 4 023 751 bp in size and contains dozens of genes involved in plant symbiosis. Curing one of the four plasmids, pPant1, resulted in a decrease in the size of the FBS135 colonies and the loss of the ability to synthesize yellow pigment, indicating that this plasmid may be very important for FBS135. CONCLUSIONS: Pantoea eucalypti FBS135 has a genomic basis to be implicated in plant-associated lifestyle and was established to have the capability to promote pine growth. SIGNIFICANCE AND IMPACT OF THE STUDY: To the best of our knowledge, this is the first report that such a bacterial species, P. eucalypti, was isolated from pine trees and evidenced to have pine beneficial activities. Our results elucidate the ecological effects of endophytes on forest plants as well as endophyte-plant interaction mechanisms.
Assuntos
Genoma Bacteriano/genética , Pantoea/fisiologia , Pinus/crescimento & desenvolvimento , Pinus/microbiologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , China , Endófitos/classificação , Endófitos/genética , Endófitos/fisiologia , Pantoea/classificação , Pantoea/genética , Plasmídeos/genética , Plasmídeos/metabolismo , RNA Ribossômico 16S/genética , Plântula/crescimento & desenvolvimento , Plântula/microbiologia , SimbioseRESUMO
OBJECTIVE: The present study was performed to examine the role of pqqE inhabiting rhizobacteria in organic acid production and relationship of the organic acids with phosphate solubilization by the bacteria in vitro as well as in vivo. METHODS AND RESULTS: The pqqE gene was PCR amplified and sequenced in genomic DNA of Pantoea sp. WP-5 and Pseudomonas sp. NN-4. Nucleotide sequence obtained from WP-5 and NN-4 showed maximum sequence similarity (88 and 89%, respectively) with the pqqE gene of Pseudomonas fluorescens strain CMR12a (KM251420). Deduced amino acid sequence from pqqE gene of Pseudomonas sp. NN-4 and Pantoea sp. WP-5 showed 75 and 93% similarity, respectively, with protein pyrroloquinoline quinone. Phosphate solubilization and acid production assay were quantified on spectrophotometer and high-profile liquid chromatograph, respectively, by each bacterial strain. Both strains produced organic acids such as acetic, citric, gluconic, succinic and malic acid and lowered the pH of Pikovskaya broth medium under laboratory conditions. Phosphate solubilization by Pantoea sp. WP-5 was 311 ± 4 and 204 ± 3 µg ml-1 in the culture medium supplemented with glucose and sucrose as carbon source, respectively. Pseudomonas sp. NN-4 solubilized 176 ± 3 and 298 ± 5 µg ml-1 phosphate in Pikovskaya broth medium under similar conditions. In field experiments conducted during two consecutive years, the concentration of acetic acid and gluconic acid was higher in root exudates of plants treated with Pantoea sp. WP-5 at 30% reduced doses of nitrogen (N)- and phosphorus (P)-based chemical fertilizers as compared to non-inoculated plants. Values of chlorophyll contents, crop growth rate, leaf area index, straw yield and P contents were recorded higher in plants inoculated with Pantoea sp. WP-5 and Pseudomonas sp. NN-4 as compared to non-inoculated control. Grain yield was increased by 10-12% due to inoculation with Pantoea sp. WP-5 and Pseudomonas sp. NN-4 over non-inoculated control in the field experiments. CONCLUSIONS: These results lead to the conclusions that the rhizobacteria inhabiting pqqE gene produced organic acids and solubilized the phosphate in vitro. On inoculation to wheat plants in field experiments, these strains produced the organic acids, solubilized the phosphate, and improved the P uptake and productivity of wheat. SIGNIFICANCE AND IMPACT OF THE STUDY: The Pantoea sp. WP-5 and Pseudomonas sp. NN-4 are the potential candidates for inoculation to wheat as phosphate solubilizer even with reduced chemical fertilizer dose. The inoculation of the strains may enhance grain yield and net income of the farmer even with less chemical fertilizer application. This practice will be helpfull inminimizing environmental pollution.
Assuntos
Proteínas de Bactérias/genética , Pantoea/fisiologia , Pseudomonas/fisiologia , Triticum/crescimento & desenvolvimento , Triticum/microbiologia , Ácidos/metabolismo , Proteínas de Bactérias/metabolismo , Grão Comestível/crescimento & desenvolvimento , Grão Comestível/metabolismo , Fertilizantes/análise , Pantoea/genética , Pantoea/metabolismo , Fosfatos/metabolismo , Pseudomonas/genética , Pseudomonas/metabolismo , Solo/química , Microbiologia do Solo , Triticum/metabolismoRESUMO
Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, we report a rare example of the induction of silent/cryptic bacterial biosynthetic pathway by the co-culture of Durum wheat plant roots-associated bacterium Pantoea aggolomerans and date palm leaves-derived fungus Penicillium citrinum. The initial co-culture indicated a clear fungal growth inhibition which was confirmed by the promising antifungal activity of the co-culture total extract against Pc. LC-HRMS chemical profiling demonstrated a huge suppression in the production of secondary metabolites (SMs) of axenic cultures of both species with the emergence of new metabolites which were dereplicated as a series of siderophores. Large-scale co-culture fermentation led to the isolation of two new pulicatin derivatives together with six known metabolites which were characterised using HRESIMS and NMR analyses. During the in vitro antimicrobial evaluation of the isolated compounds, pulicatin H (2) exhibited the strongest antifungal activity against Pc, followed by aeruginaldehyde (1) and pulicatin F (4), hence explaining the initial growth suppression of Pc in the co-culture environment.
Assuntos
Pantoea/química , Pantoea/metabolismo , Tiazolidinas/metabolismo , Antibacterianos , Anti-Infecciosos , Antifúngicos , Técnicas de Cocultura , Fermentação , Pantoea/fisiologia , Penicillium , Raízes de Plantas , SideróforosRESUMO
Numerous Pantoea strains are important because of the benefit they provide in the facilitation of plant growth. However, Pantoea have a high level of genotypic diversity and not much is understood regarding their ability to function in a plant beneficial manner. In the work reported here, the plant growth promotion activities and the genomic properties of the unusual Pantoea phytobeneficialis MSR2 are elaborated, emphasizing the genetic mechanisms involved in plant colonization and growth promotion. Detailed analysis revealed that strain MSR2 belongs to a rare group of Pantoea strains possessing an astonishing number of plant growth promotion genes, including those involved in nitrogen fixation, phosphate solubilization, 1-aminocyclopropane-1-carboxylic acid deaminase activity, indoleacetic acid and cytokinin biosynthesis, and jasmonic acid metabolism. Moreover, the genome of this bacterium also contains genes involved in the metabolism of lignin and other plant cell wall compounds, quorum-sensing mechanisms, metabolism of plant root exudates, bacterial attachment to plant surfaces and resistance to plant defences. Importantly, the analysis revealed that most of these genes are present on accessory plasmids that are found within a small subset of Pantoea genomes, reinforcing the idea that Pantoea evolution is largely mediated by plasmids, providing new insights into the evolution of beneficial plant-associated Pantoea.
Assuntos
Pantoea/fisiologia , Reguladores de Crescimento de Plantas/fisiologia , Genoma de Planta , Ácidos Indolacéticos , Fixação de Nitrogênio , Pantoea/genética , Fosfatos/metabolismo , Desenvolvimento Vegetal , Raízes de Plantas/microbiologiaRESUMO
Epiphytic bacteria have been shown to affect the composition of volatiles released by plants and as a consequence the behavior of other organisms towards the plant, such as herbivores and/or pathogens. In this study, we explored the effects of inoculation with three bacterial strains, namely Pseudomonas syringae, Pantoea ananatis, and Pseudomonas putida, on the composition of leaf volatile organic compounds (VOCs) emitted by bean plants (Phaseolus vulgaris L.). In addition, we examined responses of the two-spotted spider mite (Tetranychus urticae) to VOCs by measuring leaf damage and oviposition of female adults after bacterial inoculation. Colonized bean plants emitted different VOCs depending on the bacterial inoculum. The quantities of volatiles 1-undecanol and (Z)-3-hexen-1-ol significantly increased after P. syringae inoculation, while methyl salicylate and anisole increased in response to P. ananatis. T. urticae females preferred control plants over plants inoculated with P. syringae or P. putida in olfactometer assays, while no particular preference was recorded in the presence of P. ananatis. Furthermore, leaf damage caused by spider mites was 3-fold lower in plants inoculated with P. syringae than in control plants and plants inoculated with P. ananatis. Subsequently, the number of eggs laid on leaves inoculated with P. syringae was significantly lower than on those inoculated with P. ananatis or on the control ones. Moreover, a significantly higher number of spider mites selected methyl salicylate odor source over 1-undecanol, in a two-choice bioassay. The results demonstrate the bacterial involvement in plant-arthropod interactions and suggest further investigation on the potential use of bacteria as biocontrol agents in agriculture.
Assuntos
Pantoea/fisiologia , Phaseolus/química , Pseudomonas putida/fisiologia , Pseudomonas syringae/fisiologia , Tetranychidae/fisiologia , Compostos Orgânicos Voláteis/metabolismo , Animais , Feminino , Oviposição , Folhas de Planta/químicaRESUMO
CASE DESCRIPTION: Five workers (2 males and 3 females) employed in a furniture factory located in eastern Poland developed hypersensitivity pneumonitis (HP) after the pine wood used for furniture production was replaced by birch wood. All of them reported onset of respiratory and general symptoms (cough, shortness of breath, general malaise) after inhalation exposure to birch dust, showed crackles at auscultation, ground-glass attenuations in HRCT examination, and lymphocytosis in the BAL examination. The diagnosis of acute HP was set in 4 persons and the diagnosis of subacute HP in one. IDENTIFICATION OF SPECIFIC ALLERGEN: Samples of birch wood associated with evoking disease symptoms were subjected to microbiological analysis with the conventional and molecular methods. Two bacterial isolates were found to occur in large quantities (of the order 108 CFU/g) in examined samples: Gram-negative bacterium of the species Pantoea agglomerans and a non-filamentous Gram-positive actinobacterium of the species Microbacterium barkeri. In the test for inhibition of leukocyte migration, 4 out of 5 examined patients showed a positive reaction in the presence of P. agglomerans and 2 in the presence of M. barkeri. Only one person showed the presence of precipitins to P. agglomerans and none to M. barkeri. In the inhalation challenge, which is the most relevant allergological test in the HP diagnostics, all patients reacted positively to P. agglomerans and only one to M. barkeri. The results indicate that P. agglomerans developing in birch wood was the main agent causing HP in the workers exposed to the inhalation of dust from this wood, while the etiologic role of M. barkeri is probably secondary. CONCLUSION: The results demonstrate that apart from fungi and filamentous actinobacteria, regarded until recently as causative agents of HP in woodworkers, Gram-negative bacteria and non-filamentous actinobacteria may also elicit disease symptoms in the workers processing wood infected with large amounts of these microorganisms. The results obtained also seem to indicate that cellular-mediated reactions are more significant for causing disease symptoms compared to those that are precipitin-mediated.
Assuntos
Actinobacteria/isolamento & purificação , Poluentes Ocupacionais do Ar/efeitos adversos , Alveolite Alérgica Extrínseca/microbiologia , Betula/microbiologia , Poeira/análise , Exposição por Inalação/efeitos adversos , Pantoea/isolamento & purificação , Actinobacteria/genética , Actinobacteria/fisiologia , Doença Aguda , Adulto , Poluentes Ocupacionais do Ar/análise , Alveolite Alérgica Extrínseca/etiologia , Feminino , Humanos , Exposição por Inalação/análise , Masculino , Microbacterium , Pessoa de Meia-Idade , Pantoea/genética , Pantoea/fisiologia , Polônia , Madeira/microbiologiaRESUMO
Biocontrol offers a promising alternative to synthetic fungicides for the control of a variety of pre- and post-harvest diseases of crops. Black rot, which is caused by the pathogenic fungus Ceratocytis fimbriata, is the most destructive post-harvest disease of sweet potato, but little is currently known about potential biocontrol agents for this fungus. Here, we isolated several microorganisms from the tuberous roots and shoots of field-grown sweet potato plants, and analyzed their ribosomal RNA gene sequences. The microorganisms belonging to the genus Pantoea made up a major portion of the microbes residing within the sweet potato plants, and fluorescence microscopy showed these microbes colonized the intercellular spaces of the vascular tissue in the sweet potato stems. Four P. dispersa strains strongly inhibited C. fimbriata mycelium growth and spore germination, and altered the morphology of the fungal hyphae. The detection of dead C. fimbriata cells using Evans blue staining suggested that these P. dispersa strains have fungicidal rather than fungistatic activity. Furthermore, P. dispersa strains significantly inhibited C. fimbriata growth on the leaves and tuberous roots of a susceptible sweet potato cultivar ("Yulmi"). These findings suggest that P. dispersa strains could inhibit black rot in sweet potato plants, highlighting their potential as biocontrol agents.
Assuntos
Ascomicetos/crescimento & desenvolvimento , Ipomoea batatas/imunologia , Pantoea/fisiologia , Controle Biológico de Vetores , Doenças das Plantas/prevenção & controle , Folhas de Planta/imunologia , Raízes de Plantas/imunologia , Resistência à Doença , Ipomoea batatas/microbiologia , Doenças das Plantas/imunologia , Doenças das Plantas/microbiologia , Folhas de Planta/microbiologia , Raízes de Plantas/microbiologiaRESUMO
Vascular wilt bacteria such as Pantoea stewartii, the causal agent of Stewart's bacterial wilt of maize (SW), are destructive pathogens that are difficult to control. These bacteria colonize the xylem, where they form biofilms that block sap flow leading to characteristic wilting symptoms. Heritable forms of SW resistance exist and are used in maize breeding programs but the underlying genes and mechanisms are mostly unknown. Here, we show that seedlings of maize inbred lines with pan1 mutations are highly resistant to SW. However, current evidence suggests that other genes introgressed along with pan1 are responsible for resistance. Genomic analyses of pan1 lines were used to identify candidate resistance genes. In-depth comparison of P. stewartii interaction with susceptible and resistant maize lines revealed an enhanced vascular defense response in pan1 lines characterized by accumulation of electron-dense materials in xylem conduits visible by electron microscopy. We propose that this vascular defense response restricts P. stewartii spread through the vasculature, reducing both systemic bacterial colonization of the xylem network and consequent wilting. Though apparently unrelated to the resistance phenotype of pan1 lines, we also demonstrate that the effector WtsE is essential for P. stewartii xylem dissemination, show evidence for a nutritional immunity response to P. stewartii that alters xylem sap composition, and present the first analysis of maize transcriptional responses to P. stewartii infection.
Assuntos
Resistência à Doença , Pantoea , Zea mays , Resistência à Doença/genética , Genoma de Planta/genética , Pantoea/fisiologia , Plântula/microbiologia , Xilema/microbiologia , Zea mays/genética , Zea mays/microbiologiaRESUMO
Lead can be immobilized in contaminated soils by phosphate rock (PR) amendment, but its efficiency is generally limited by low solubility of PR. Our study aimed to elucidate whether phosphate solubilizing bacteria (PSB) can promote Pb immobilization through PR solubilization. Results showed that P. ananatis HCR2 and B. thuringiensis GL-1 could effectively solubilize PR by producing citric, glucose, and α-Ketoglutaric acids. In broth assay, phosphate solubilized from PR by PSB rapidly reacted with Pb2+ and formed insoluble lead compounds, as confirmed by scanning electron microscope, energy dispersive X-ray, and X-ray photoelectron spectroscopy. Pot experiment using lettuce (Lactuca sativa L.) and diffusive gradients in thin films (DGT) verified the effectiveness of soil remediation using PR amendment and PSB inoculation, as plant shoot biomass and net photosynthetic rate as well as soil bioavailable phosphate concentration have significantly increased, while the phytoavailability of Pb, Cd, and Zn greatly reduced. This study suggested that PR amendment combined with PSB inoculation could be applied for remediation of agricultural fields contaminated with multiple heavy metals.