Simulated gastric fluid assay for estimating the digestibility of newly expressed proteins in GE crops: Missteps in development and interpretation.

Digestive stability of a food protein in simulated gastric fluid (SGF) continues to be considered a risk factor for allergy, even though the current science does not support this belief. Methodological shortcomings of the adaption of the SGF assay for use with purified proteins has been cited as a reason to discount results that do not conform to this belief. Missteps in conducting and interpreting the results of SGF assays are reviewed here. However, these methodological shortcomings do not invalidate the conclusion that allergenic proteins are not systematically more stable to digestion than non-allergens. The growing evidence for the dual allergen exposure hypothesis, whereby sensitization to food allergens is primarily caused by dermal and inhalation exposure to food dust, and tolerization against food allergy is primarily induced by gut exposure in food, likely explains why the digestive stability of a protein is not a risk factor for allergenicity.

Zinc oxide nanoparticles alleviates the adverse effects of cadmium stress on Oryza sativa via modulation of the photosynthesis and antioxidant defense system.

Cadmium (Cd) is a trace element causing severe toxicity symptoms in plants, besides posing hazardous fitness issue due to its buildup in the human body through food chain. Nanoparticles (NPs) are recently employed as a novel strategy to directly ameliorate the Cd stress and acted as nano-fertilizers. The intend of the current study was to explore the effects of zinc oxide nanoparticles (ZnO-NPs; 50 mg/L) on plant growth, photosynthetic activity, elemental status and antioxidant activity in Oryza sativa (rice) under Cd (0.8 mM) stress. To this end, the rice plants are treated by Cd stress at 15 days after sowing (DAS), and the treatment was given directly into the soil. Supply of ZnO-NPs as foliar spray was given for five consecutive days from 30 to 35 DAS, and sampling was done at 45 DAS. However, rice plants supplemented with ZnO-NPs under the Cd toxicity revealed significantly increased shoot length (SL; 34.0%), root fresh weight (RFW; 30.0%), shoot dry weight (SDW; 23.07%), and root dry weight (RDW; 12.24%). Moreover, the ZnO-NPs supplement has also positive effects on photosynthesis related parameters, SPAD value (40%), chloroplast structure, and qualitatively high fluorescence observed by confocal microscopy even under Cd stress. ZnO-NPs also substantially prevented the increases of hydrogen peroxide (H2O2) and malondialdehyde (MDA) triggered by Cd. Physiological and biochemical analysis showed that ZnO-NPs increased enzymatic activities of superoxide dismutase (SOD; 59%), catalase (CAT; 52%), and proline (17%) that metabolize reactive oxygen species (ROS); these increases coincided with the changes observed in the H2O2 and MDA accumulation after ZnO-NPs application. In conclusion, ZnO-NPs application to foliage has great efficiency to improve biomass, photosynthesis, protein, antioxidant enzymes activity, mineral nutrient contents and reducing Cd levels in rice. This can be attributed mainly from reduced oxidative damage resulted due to the ZnO-NPs application.

Mass spectrometric analysis of digesta does not improve the allergenicity assessment of GM crops.

An investigation of the potential allergenicity of newly expressed proteins in genetically modified (GM) crops comprises part of the assessment of GM crop safety. However, allergenicity is not completely predictable from a definitive assay result or set of protein characteristics, and scientific opinions regarding the data that should be used to assess allergenicity are continuously evolving. Early studies supported a correlation between the stability of a protein exposed to digestive enzymes such as pepsin and the protein's status as a potential allergen, but over time the conclusions of these earlier studies were not confirmed. Nonetheless, many regulatory authorities, including the European Food Safety Authority (EFSA), continue to require digestibility analyses as a component of GM crop risk assessments. Moreover, EFSA has recently investigated the use of mass spectrometry (MS), to make digestion assays more predictive of allergy risk, because it can detect and identify small undigested peptides. However, the utility of MS is questionable in this context, since known allergenic peptides are unlikely to exist in protein candidates intended for commercial development. These protein candidates are pre-screened by the same bioinformatics processes that are normally used to identify MS targets. Therefore, MS is not a standalone allergen identification method and also cannot be used to predict previously unknown allergenic epitopes. Thus, the suggested application of MS for analysis of digesta does not improve the poor predictive power of digestion assays in identifying allergenic risk.

Lay people and experts' risk perception of pharmaceuticals in the environment in Southwestern Europe.

This study aims to contribute to the risk management of pharmaceuticals in the environment, illustrating risk perceptions of lay people and experts from Southwestern Europe (Portugal, Spain, and France). The psychometric paradigm was applied to assess risk regarding four hazards: pharmaceuticals in the environment (i.e., broadly framed), pharmaceuticals in treated wastewater, pharmaceuticals in drinking water, and pharmaceuticals in crops. Two factors explained most of the variance of risk assessments: dread and unknown. The dread factor combined immediacy and severity of effects, and the old nature of hazards. Pharmaceuticals in crops and drinking water scored higher in this factor, as did experts and French respondents. The unknown factor differentiated between the assessments of lay people and experts. Lay people assessed the hazards as being more known by those who were exposed but less known by science; and exposure was perceived as more voluntary and the risk as more controllable. Even though pharmaceutical residues are present in much higher concentrations in treated wastewater, risk assessments were overall higher for drinking water and crops. Moreover, data also revealed risk management preferences: whereas lay people preferred technological and awareness-type measures, experts preferred measures to improve the disposal of pharmaceutical waste and health-type measures.

Results from ten years of post-market environmental monitoring of genetically modified MON 810 maize in the European Union.

In European regulations for the deliberate release into the environment of genetically modified organisms (GMO), the objective of General Surveillance in Post-Market Environmental Monitoring is defined as the identification of the occurrence of adverse effects of the GMO or its use which were not anticipated in the environmental risk assessment (ERA). Accompanying the commercial cultivation in the EU of maize event MON 810, General Surveillance was implemented by Monsanto, the authorization holder, on a voluntary basis. We carried out a statistical analysis on the pooled results of ten years of farmer questionnaires, which were a part of this General Surveillance, amounting to 2,627 farmer fields in eight European countries in the period 2006-2015. This analysis did not reveal any unexpected adverse effects associated with the cultivation of MON 810. Results from farmer questionnaires confirmed that the cultivation of MON 810 resulted in a significant reduction in the use of pesticides, efficient protection against the target pests, and healthier, higher yielding crops compared to conventional maize. MON 810 also had reduced susceptibility to disease and pests when compared to conventional maize. Monitoring characteristics related to environment and wildlife revealed no significant differences between MON 810 and conventional maize. Literature searches, that were also conducted as part of General Surveillance, identified a comprehensive set of publications addressing environmental safety as well as food and feed safety aspects of MON 810. None of the publications indicated any adverse effect of MON 810 that was not anticipated in the initial ERA, nor did they lead to a change in the conclusions of the initial risk assessment that demonstrated the safety of MON 810. The development of resistance by the target pests (Ostrinia nubilalis, ECB and Sesamia nonagrioides, MCB) was the only potential adverse effect identified in the ERA of MON 810 cultivation in the EU. The extensive safety data package for MON 810, the robust weight of evidence demonstrating both its safety and benefits, and the history of safe use of MON 810 for 15 years in the EU, indicates that focussing the General Surveillance of MON 810 on literature searches and farmer complaint systems would be appropriately protective. This will allow the identification of potential adverse effect not anticipated in the initial ERA without the intensive effort and organizational challenges of farmer questionnaires.

Soil-plant system and potential human health risk of Chinese cabbage and oregano growing in soils from Mn- and Fe-abandoned mines: microcosm assay.

In Portugal, many abandoned mines are often close to agricultural areas and might be used for plant food cultivation. Soils in the vicinity of two Mn- and Fe-abandoned mines (Ferragudo and Rosalgar, SW of Portugal) were collected to cultivate two different food species (Brassica rapa subsp. pekinensis (Lour.) Hanelt and Origanum vulgare L.). Chemical characterization of the soil-plant system and potential risk of adverse effects for human health posed by plants associated with soil contamination, based on the estimation of hazard quotient (HQ), were assessed in a microcosm assay under greenhouse conditions. In both soils, the average total concentrations of Fe and Mn were above the normal values for soils in the region and their concentration in shoots of both species was very high. Brassica rapa subsp. pekinensis grew better in Ferragudo than in Rosalgar soils, and it behaved as an excluder of Cu, Mn, Fe, S and Zn in both soils. The HQ for Cu, Fe, Mn and Zn in the studied species grown on both soils was lower than unit indicating that its consumption is safe. The high Mn tolerance found in both species might be due in part to the high contents of Fe in the soil available fraction that might contribute to an antagonism effect in the uptake and translocation of Mn. The obtained results emphasize the need of further studies with different food crops before cultivation in the studied soils to assess health risks associated with high metal intake.

Cumulative risk assessment of pesticide residues in different Iranian pistachio cultivars: Applying the source specific HQS and adversity specific HIA approaches in Real Life Risk Simulations (RLRS).

Iran is a major supplier of the world pistachio market. In this study, we collected five pistachio cultivars from four main pistachio-producing zones in August and September 2016, and determined the residues of 18 organophosphorus, carbamate, pyrethroid and nicotinoid pesticide in these samples using Gas Chromatography-Mass Spectrometry, as an efficient method for determination of pesticides' residues. Next, single-chemical and chronic cumulative risk assessment was done based on the new approaches of the food specific Hazard Quotient and adversity specific Hazard Index. Fifteen from eighteen food-specific Hazard Quotients were above 1 even in cases when the respective contamination was bellow MRLs. The adversity specific Hazard Indexes values were above 1 for five from six adversities indicating various risks in the resulted levels of pistachios' contamination from the pesticides' mixture. However, no risk for carcinogenicity was found. Our results indicate the necessity of taking appropriate measures to control/standardize pesticides practice in pistachio cultivation in Iran and the need to re-establish the MRLs based on cumulative exposure.

Risk-Only Assessment of Genetically Engineered Crops Is Risky.

The risks of not considering benefits in risk assessment are often overlooked. Risks are also often evaluated without consideration of the broader context. We discuss these two concepts in relation to genetically engineered (GE) crops. The health, environmental, and economic risks and benefits of GE crops are exemplified and presented in the context of modern agriculture. Misattribution of unique risks to GE crops are discussed. It is concluded that the scale of modern agriculture is its distinguishing characteristic and that the greater knowledge around GE crops allows for a more thorough characterization of risk. By considering the benefits and risks in the context of modern agriculture, society will be better served and benefits will be less likely to be forgone.

Cercas vivas en sistemas de producción tropicales: una revisión mundial de los usos y percepciones / Live fences in tropical production systems: a global review of uses and perceptions

Resumen La expansión de la producción agrícola y ganadera ha sido un factor clave en la deforestación en el mundo y especialmente en la región tropical. En consecuencia, más de la mitad de la superficie terrestre del planeta se utiliza para el establecimiento de sistemas productivos, en este contexto las cercas vivas pueden generan efectos positivos sobre el ambiente y proveer bienes y servicios ambientales. En este trabajo hacemos una revisión sobre los efectos que tienen las cercas vivas sobre los sistemas productivos, analizando los usos y percepciones que tienen los agricultores. Esta revisión abarca las diferentes regiones tropicales del mundo. Se encontró que las cercas vivas estuvieron principalmente vinculadas a la delimitación de predios y potreros. No obstante, sus usos y beneficios son múltiples, entre los que se encontraron suministrar forraje, extracción de madera, proveer frutos, servir como rompevientos, generar medicinas, evitar la erosión del suelo, retener la humedad y suministrar aportes nutricionales. Además, se encontraron percepciones a favor como aumentar la conectividad del paisaje, aumentar los ingresos económicos, reducir los costos productivos, reducir la presión sobre la vegetación remanente, tener bajo costos de mantenimiento, contribuir al control de plagas y mejoran la fertilidad. Sin embargo, también se encontraron percepciones negativas como el excesivo trabajo al repararlas o podarlas, la sombra puede afectar la producción agrícola, la falta de información desestimula su establecimiento, costos elevados de establecimiento, generación de conflictos de propiedades y dificultad de manejo. Se encontró que los usos por parte de los agricultores están enfocados a mejorar los rendimientos de los sistemas productivos, pero también a la conservación de recursos naturales como el suelo y la biodiversidad. Se proponen temas de investigación para el futuro como determinar los criterios en la elección de especies nativas para establecer y enriquecer las cercas vivas dando valor a aspectos ecológicos y socioeconómicos, además, se debe profundizar en los beneficios que producen sobre los rendimientos de los sistemas productivos Se sugiere realizar estudios sobre las cercas vivas en la región tropical de Australasia debido a que no se encontraron estudios en esta región, así se podría conocer el estado de su biodiversidad y servicios que brinda a la comunidad rural. Por último, lo que se busca con esta revisión es generar iniciativas que fomenten la formulación de políticas rurales, la creación de programas que estimulen el establecimiento de cercas vivas, ya sea por medio de pagos por servicios ambientales, compensaciones tributarias u otro tipo de mecanismos. Proveer información adecuada a agricultores y ganaderos es un aspecto central de dinamiza el establecimiento de las cercas vivas.

Abstract The expansion of agricultural and livestock production has been a key factor in deforestation in the world and especially in the tropical region. Currently, more than half of the Earth's surface is used for the establishment of production systems; in this context, live fences can generate positive effects on the environment and provide environmental goods and services. In this work, we reviewed the effects of live fences on production systems from the tropical regions of America, Africa, Asia and Australia, analyzing the uses and perceptions of farmers that have made use of these fences. It was found that live fences were mainly related to the delimitation of farms and pastures. However, their uses and benefits are manifold, among which we found: provide forage, wood extraction, fruit supply, windbreaks, medicinal, to avoid soil erosion, moisture retention and nutritional support. In addition, other positive perceptions were: increasing landscape connectivity, increasing economic incomes, reducing production costs, reducing pressure on remaining vegetation, keeping maintenance costs down, contributing to pest control, and improving fertility. However, negative perceptions included the excessive work in repairing or pruning them, shade negative impact on agricultural production, lack of information discourages establishment, high establishment costs, generation of property conflicts and management difficulty. It was found that the uses by farmers are focused on improving the yields of productive systems, but also on the conservation of natural resources especially soil and biodiversity. We proposed research topics for the future, as determining the criteria in the selection of native species to establish and enrich living fences valuing ecological and socioeconomic aspects; in additionally, we must deepen the benefits they produce on the yields of productive systems. We suggest the development of live fences studies in the tropical region of Australasia, because no data was found for this region, in order to know the biodiversity and services status that they provide to the rural communities. As a conclusion, it will be important to generate initiatives that encourage the formulation of rural policies, the creation of programs that encourage the establishment of live fences, whether through payments for environmental services, tax compensation or other mechanisms. Providing adequate information to farmers and livestock farmers is a key aspect on dynamizing the establishment of live fences. Rev. Biol. Trop. 66(2): 739-753. Epub 2018 June 01.

Stakeholders' Perceptions of Agronomic Iodine Biofortification: A SWOT-AHP Analysis in Northern Uganda.

Agronomic biofortification (i.e., the application of fertilizer to elevate micronutrient concentrations in staple crops) is a recent strategy recommended for controlling Iodine Deficiency Disorders (IDDs). However, its success inevitably depends on stakeholders' appreciation and acceptance of it. By taking Northern Uganda as a case, this study aimed to capture and compare the perceptions of seven key stakeholder groups with respect to agronomic iodine biofortification. Therefore, we employed a SWOT (Strength, Weaknesses, Opportunities & Threats) analysis in combination with an Analytical Hierarchy Process (AHP). Findings show that stakeholders (n = 56) are generally positive about agronomic iodine biofortification in Uganda, as its strengths and opportunities outweighed weaknesses and threats. Cultural acceptance and effectiveness are considered the most important strengths while the high IDD prevalence rate and the availability of iodine deficient soils are key opportunities for further developing agronomic iodine biofortification. Environmental concerns about synthetic fertilizers as well as the time needed to supply iodine were considered crucial weaknesses. The limited use of fertilizer in Uganda was the main threat. While this study provides insight into important issues and priorities for iodine biofortification technology in Uganda, including differences in stakeholder views, the application of the SWOT-AHP method will guide future researchers and health planners conducting stakeholder analysis in similar domains.

2015 Evidence Analysis Library Systematic Review on Advanced Technology in Food Production.

In the late 20th century, plant breeders began using molecular biology techniques such as recombinant DNA, also known as genetic engineering, along with traditional cross-breeding. Ten plant and one animal food have been approved for commercialization in the United States. Today, foods and ingredients from genetically engineered (GE) crops are present throughout the food supply, which has led to varying levels of acceptance. Much discussion exists among consumers and health professionals about the believability of statements made regarding benefits or risks of GE foods. The aim of this systematic review was to examine the evidence on the association of consumption of GE foods and ingredients derived from them on human health, specifically allergenicity, food safety, pesticide consumption, nutrient adequacy, inflammation, and antibiotic resistance. An expert panel conducted a systematic review on advanced technology in food production. The 30 developed questions focused on effects of human consumption of GE foods and the effects of human consumption of foods containing pesticide residues on human health. Primary research published from 1994 to 2014 were identified using PubMed and Agricultural Online Access databases. Additional studies were identified by searching references of review articles. Twenty-one studies met the inclusion criteria. Relevant research addressed five of 30 questions. Four questions focused on food allergenicity, the fifth on nutrient adequacy, and all received a Grade III (limited/weak) rating. No human studies addressed 25 questions on the consumption of foods produced using genetic engineering technologies on gene translocation, cancer, food safety, phenotype expression, inflammation and inflammatory markers, or antibiotic resistance. These questions received a Grade V (grade not assignable). Evidence from human studies did not reveal an association between adverse health effects and consumption of foods produced using genetic engineering technologies. Although the number of available human studies is small, they support that there are no clear adverse health effects-as they relate to allergenicity and nutrient adequacy-associated with consumption of GE foods. The present systematic review is aligned with a recent report by the National Academy of Sciences that included human and animal research.

Changes in the Anti-Allergic Activities of Sesame by Bioconversion.

Sesame is an important oilseed crop, which has been used as a traditional health food to ameliorate the prevention of various diseases. We evaluated the changes in the anti-allergic activities of sesame by bioconversion. SDS-PAGE of non-fermented sesame proteins showed major allergen bands, while that of fermented sesame showed only a few protein bands. Additionally, we investigated the effectiveness of fermented sesame by bioconversion in tumor necrosis factor-α (TNF-α)- and interferon-γ (IFN-γ)-induced HaCaT cells. In HaCaT cells, fermented sesame inhibited the mRNA expression of interleukin-6 (IL-6) and interleukin-1ß (IL-1ß), thymus and macrophage-derived chemokine (MDC/CCL22), activation-regulated chemokine (TARC/CCL17), and intercellular adhesion molecule-1 (ICAM-1). Moreover, fermented sesame inhibited the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 1 (STAT1). Fermented sesame exerts anti-allergic effects by suppressing the expression of chemokines and cytokines via blockade of NF-κB and STAT1 activation.

Safety assessment of transgenic canola RF3 with bar and barstar gene on Sprague-Dawley (SD) rats by 90-day feeding test.

Canola is one of the most important plant oilseed crops. To avoid the threat of herbicides, the RF3 line with bar gene and barstar gene was developed, which can act as glufosinate resistance resources and restore fertility in hybrid lines. To assess the food safety of transgenic canola RF3, 2.5%, 5% and 10% GM canola RF3 and its non-GM isogenic line Drakkar were formulated into diet to feed Spragure-Dawley (SD) rats for 90 days. The effects on the general growth and toxicological parameters, as well as gut microbiota of rats, were evaluated. Several significant differences on body weight, feed consumption, relative organ weight, hematology and serum biochemistry were observed among rats in the 90-day feeding test. However, these statistical differences were randomly observed among different groups and were not dose-related, which were not considered to be biologically significant. Furthermore, the results of bacterial 16S rRNA sequencing of fecal samples showed that the diets containing GM canola did not disturb the balance of gut microbiota. In conclusion, the canola RF3 is considered as safe and wholesome as the non-GM canola based on this 90-day feeding test and gut microbiota analysis.

Characterization of scientific studies usually cited as evidence of adverse effects of GM food/feed.

GM crops are the most studied crops in history. Approximately 5% of the safety studies on them show adverse effects that are a cause for concern and tend to be featured in media reports. Although these reports are based on just a handful of GM events, they are used to cast doubt on all GM crops. Furthermore, they tend to come from just a few laboratories and are published in less important journals. Importantly, a close examination of these reports invariably shows methodological flaws that invalidate any conclusions of adverse effects. Twenty years after commercial cultivation of GM crops began, a bona fide report of an adverse health effect due to a commercialized modification in a crop has yet to be reported.

Vulnerability of global food production to extreme climatic events.

It is known that the frequency, intensity or duration of the extreme climatic events have been changing substantially. The ultimate goal of this study was to identify current vulnerabilities of global primary food production against extreme climatic events, and to discuss potential entry points for adaptation planning by means of an explorative vulnerability analysis. Outcomes of this analysis were demonstrated as a composite index where 118 country performances in maintaining safety of food production were compared and ranked against climate change. In order to better interpret the results, cluster analysis technique was used as a tool to group the countries based on their vulnerability index (VI) scores. Results suggested that one sixth of the countries analyzed were subject to high level of exposure (0.45-1), one third to high to very high level of sensitivity (0.41-1) and low to moderate level of adaptive capacity (0-0.59). Proper adaptation strategies for reducing the microbial and chemical contamination of food products, soil and waters on the field were proposed. Finally, availability of data on food safety management systems and occurrence of foodborne outbreaks with global coverage were proposed as key factors for improving the robustness of future vulnerability assessments.

Human health risks related to the consumption of foodstuffs of plant and animal origin produced on a site polluted by chemical munitions of the First World War.

Shells fired during World War I exhibited different explosive compounds and some of these weapons also contained a wide variety of chemical warfare agents. At the end of the war, for safety purposes, the large quantity of weapons remaining on the former front needed to be dismantled and destroyed. A large amount of the remaining shells was destroyed in specific sites which led to the contamination of the surroundings in Belgium and France. In the 1920s, 1.5 million chemical shells and 30,000 explosive shells were destroyed in a place close to the city of Verdun, in the East of France. In this paper, the risk for human health related to the consumption of foodstuffs produced on this site was assessed. To this end, food products of plant and animal origin were sampled in 2015-2016 and contaminant analyses were conducted. Human exposure was assessed using a specifically built methodology. The contaminants considered in this study were trace elements (TEs - primarily Zn, As, Pb and Cd), nitroaromatic explosives (trinitrotoluene, 2,4-dinitrotoluene, 2,6-dinitrotoluene, 2-amino-4,6-dinitroluene and 4-amino-2,6-dinitrotoluene), phenylarsenic compounds including diphenylarsinic acid and triphenylarsine, perchlorate, tetrabromoethane and vinyl bromide. Depending on the compound, different approaches were used to assess the risk for both adults and children. Exposure to these contaminants through the consumption of foodstuffs produced locally on the considered site was unlikely to be a health concern. However, as for inorganic arsenic, given the presence of highly contaminated zones, it was suggested that cereals should not be grown on certain plots.

Messages promoting genetic modification of crops in the context of climate change: Evidence for psychological reactance.

Genetic modification (GM) of crops and climate change are arguably two of today's most challenging science communication issues. Increasingly, these two issues are connected in messages proposing GM as a viable option for ensuring global food security threatened by climate change. This study examines the effects of messages promoting the benefits of GM in the context of climate change. Further, it examines whether explicit reference to "climate change," or "global warming" in a GM message results in different effects than each other, or an implicit climate reference. An online sample of U.S. participants (N = 1050) were randomly assigned to one of four conditions: "climate change" cue, "global warming" cue, implicit cue, or control (no message). Generally speaking, framing GM crops as a way to help ensure global food security proved to be an effective messaging strategy in increasing positive attitudes toward GM. In addition, the implicit cue condition led to liberals having more positive attitudes and behavioral intentions toward GM than the "climate change" cue condition, an effect mediated by message evaluations.

Avaliação da vestimenta-padrão utilizada durante a colheita das folhas do tabaco e implicações na prevenção da Green Tobacco Sickness (GTS) / Evaluation of the standard protection clothing used during tobacco harvest and implications for the Green Tobacco Sickness (GTS) prevention

Contexto: No Brasil, confirmou-se recentemente a ocorrência da Green Tobacco Sickness (GTS) em trabalhadores de uma importante região produtora de tabaco, evidenciando a necessidade da adoção de medidas preventivas para o seu controle. Objetivos: O objetivo do presente estudo foi avaliar a eficácia de uma vestimenta-padrão, proposta para uso durante a colheita das folhas de tabaco, além de avaliar a sua aplicabilidade na prevenção da GTS. Métodos: Foi realizada a quantificação da exposição potencial dérmica dos trabalhadores à nicotina e à cotinina por dosimetria passiva. Participaram do estudo 18 trabalhadores, que utilizaram a vestimenta de proteção padrão durante um dia típico de trabalho. A quantificação dos resíduos de nicotina e cotinina foi realizada por meio de UPLC-MS-MS. Resultados: Os resultados mostraram que a vestimenta conferiu proteção de cerca de 98%, e que seu uso pode contribuir para a prevenção da GTS. Conclusão: Ações de conscientização quanto à importância do uso da vestimenta, associadas ao esclarecimento sobre a doença, são os caminhos para a prevenção e o controle.

Context: Recently, Green Tobacco Sickness (GTS) has been confirmed among workers in a major tobacco-producing region in Brazil. This indicates the need to adopt preventive measures to control the disease. Objectives: This study aimed at evaluating the efficacy of the proposed use of standard clothing during tobacco leaf harvesting process, and at evaluating its applicability in the GTS prevention. Methods: Potential dermal exposure of workers to nicotine and cotinine was determined by passive dosimetry. A total of 18 workers participated in the study. These workers wore the standard protective clothing during a typical workday. Quantification of the nicotine and cotinine residues was carried out by means of UPLC-MS-MS. Results: The results showed that the clothing provided approximately 98% protection, and that its use may contribute to the GTS prevention. Conclusion: To raise awareness on the importance of the use of the clothing, associated with educational actions concerning the disease, will be the path to prevention and control of GTS.

Aspergillus and aflatoxin in groundnut (Arachis hypogaea L.) and groundnut cake in Eastern Ethiopia.

This study was conducted to assess major Aspergillus species and aflatoxins associated with groundnut seeds and cake in Eastern Ethiopia and evaluate growers' management practices. A total of 160 groundnut seed samples from farmers' stores and 50 groundnut cake samples from cafe and restaurants were collected. Fungal isolation was done from groundnut seed samples. Aspergillus flavus was the dominant species followed by Aspergillus parasiticus. Aflatoxin analyses of groundnut seed samples were performed using ultra performance liquid chromatography; 22.5% and 41.3% of samples were positive, with total aflatoxin concentrations of 786 and 3135 ng g-1 from 2013/2014 and 2014/2015 samples, respectively. The level of specific aflatoxin concentration varied between 0.1 and 2526 ng g-1 for B2 and B1, respectively. Among contaminated samples of groundnut cake, 68% exhibited aflatoxin concentration below 20 ng g-1, while as high as 158 ng g-1 aflatoxin B1 was recorded. The study confirms high contamination of groundnut products in East Ethiopia.