1.
Circ Res
; 129(4): 458-470, 2021 08 06.
Artigo
em Inglês
| MEDLINE
| ID: mdl-34176299
RESUMO
[Figure: see text].
Assuntos
Doença da Artéria Coronariana/metabolismo , Proteína ADAMTS7/química , Proteína ADAMTS7/genética , Proteína ADAMTS7/metabolismo , Animais , Domínio Catalítico , Células Cultivadas , Doença da Artéria Coronariana/genética , Células HEK293 , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Músculo Liso Vascular/metabolismo , Mutação , Polimorfismo de Nucleotídeo Único
2.
Methods Mol Biol
; 2043: 63-73, 2020.
Artigo
em Inglês
| MEDLINE
| ID: mdl-31463903
RESUMO
ADAMTS7 is a secreted protease that is predominantly expressed in tissues of the cardiovascular system and tendon. Although recent evidence suggests that it may play a role in the etiology of coronary artery disease, its physiological function and substrates are unknown. The enzyme undergoes extensive posttranslational modifications, including chondroitin sulfate attachment, N and O-linked glycosylation, and a two-step activation process. For the benefit of scientists who study the function of ADAMTS7 and its role in disease, this chapter provides an introduction to the chemical and functional properties of the various ADAMTS7 domains, as well as a protocol for the recombinant expression and purification of ADAMTS7.
