Assuntos
Abscesso Encefálico/microbiologia , Abscesso Encefálico/patologia , Hemorragia Cerebral/complicações , Hemorragia Cerebral/patologia , Enterococcus faecalis/patogenicidade , Infecções por Bactérias Gram-Positivas/etiologia , Abscesso Encefálico/diagnóstico por imagem , Causalidade , Hemorragia Cerebral/diagnóstico por imagem , Protocolos Clínicos , Complicações do Diabetes , Enterococcus faecalis/efeitos dos fármacos , Febre/complicações , Febre/microbiologia , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Humanos , Hipertensão/complicações , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Penicilinas/uso terapêutico , Putamen/diagnóstico por imagem , Putamen/microbiologia , Putamen/patologia , Tomografia Computadorizada por Raios XRESUMO
We examined the axonal transport of two strains of herpes simplex virus 1 (HSV-1) within the central nervous system of cebus monkeys. Each strain was injected into the "arm area" of the primary motor cortex. One strain, HSV-1(McIntyre-B), was transported transneuronally in the retrograde direction. It infected neurons at sites known to project to the arm area of the primary motor cortex (e.g., ventrolateral thalamus). In addition, "second-order" neurons were labeled in the deep cerebellar nuclei (dentate and interpositus) and in the globus pallidus (internal segment). This result supports the concept that the arm area of the primary motor cortex is a target of both cerebellar and basal ganglia output. In contrast, the other strain, HSV-1(H129), was transported transneuronally in the anterograde direction. It infected neurons at sites known to receive input from the arm area of the primary motor cortex (e.g., putamen, pontine nuclei). In addition, "third-order" neurons were labeled in the cerebellar cortex (granule and Golgi cells) and in the globus pallidus (largely the external segment). Our observations suggest that strain differences have an important impact on the direction of transneuronal transport of HSV-1. Furthermore, it should be possible to examine the organization of cerebellar and basal ganglia loops with cerebral cortex by exploiting transneuronal transport of HSV-1 and virus strain differences.
Assuntos
Encéfalo/microbiologia , Neurônios/microbiologia , Simplexvirus/classificação , Animais , Transporte Biológico , Encéfalo/citologia , Encéfalo/metabolismo , Cebus , Córtex Cerebelar/citologia , Córtex Cerebelar/metabolismo , Córtex Cerebelar/microbiologia , Córtex Cerebral/citologia , Córtex Cerebral/metabolismo , Córtex Cerebral/microbiologia , Globo Pálido/citologia , Globo Pálido/metabolismo , Globo Pálido/microbiologia , Córtex Motor/citologia , Córtex Motor/metabolismo , Córtex Motor/microbiologia , Neurônios/citologia , Neurônios/metabolismo , Putamen/citologia , Putamen/metabolismo , Putamen/microbiologiaRESUMO
Light and electron microscopic observation 3--4 days after microinjection of Herpes simplex virus (HSV) into the left neostriatum of rat demonstrated the following results. (1) Virus labeled nerve cells were found in the ipsilateral substantia nigra; a large number of infected neurons were in the zona compacta and some were in the zona reticulata. No virus infection was evident in the contralateral side. (2) Virus labeled neurons were found in the cortex, a greater number ipsilaterally than contralaterally, and in the dorsal raphé nuclei. Cortical microinjection of HSV led to infection of some cortical cells but no neostriatal cells. We conclude, therefore, that spread of the virus to the cortex, the substantia nigra and the dorsal raphé following neostriatal injection was by retrograde axonal transport. (3) The left neostriatum, where HSV was injected, showed a surprisingly small number of virus infected neurons. The infected neurons were mostly the large neurons; the majority of medium sized neurons were well preserved. There was massive degeneration of nerve terminals throughout the neuropil. Most of these degenerating nerve terminals are considered to be afferent fibers.
