Porotic hyperostosis and cribra orbitalia in samples from the Late Hillfort Period sites at Detkovice - Za zahradama (district Prostejov) and Vídenská street (district Brno), Czech Republic.

Porotic hyperostosis and cribra orbitalia are pathological changes occurring on the human skull. These changes were observed and evaluated on skeletal remains from Detkovice - Za zahradama and Vídenská Street in Brno; both sites are dated back to the 10th to 12th centuries AD. A total of 605 subjects were assessed for age, sex, and the above-stated pathologies using standard methods. The influence of age and sex on the occurrence of these pathologies was examined statistically. Results indicated that at the site of Detkovice - Za zahradama, porotic hyperostosis, and cribra orbitalia do not depend on sex or age. However, at Vídenská Street in Brno, these pathologies do not depend on sex, but they depend on age so a higher incidence of pathologies in juveniles is observed. Differences between both sites could be caused by different numbers of evaluated individuals or different state of preservation of skeletal remains. The aetiology of the origin of these pathologies could not be determined by the methodology used here, but with the prevailing lower age of pathological subjects, a lack of nutrition with consequent absence of iron and developing anaemia might be the cause. Based on our statistical data, we can observe that the pathologies we studied occur more frequently in children older than newborns and younger infants. This may indicate that these studied pathologies arise only during the lifetime of the individual and do not have a prenatal occurrence.

Manifestation of infantile scurvy in a skeleton from the high medieval village of Trutmanice (South Moravia, Czech Republic).

The study describes the pathological findings recorded on the human remains of a 3 to 4-year-old child found in the burial site of Trutmanice (Czech Republic), dated to the 13th-15th century. The human remains were examined using standard macroscopic osteological and paleopathological methods, supplemented by radiographic examination. Although the preservation of the bones limited the analysis, multiple pathological changes were observed mainly on the skull: cribra orbitalia, small pores and fine deposits of newly formed bone tissue, hypertrophic diploë, grooves indicating rich branching of meningeal arteries on the intracranial surface of the cranial vault. A very fine periostotic deposition of newly formed bone tissue was also observed at the distal end of the diaphysis of the right tibia and both distal ends of the femora. The radiographs of the femora showed white lines of Frankel, scurvy lines, and Wimberger's rings. The observed pathological changes were possibly consistent with scurvy based on differential diagnosis. Reports about cases of juvenile scurvy from rural areas within the context of landlocked countries of Central Europe are rare. While scurvy is now more frequently reported in the paleopathological literature, evidence for scurvy within one of the most natural agricultural areas in the Czech lands remains rare. We recommend using radiographic examination to complement the diagnosis of scurvy in future paleopathological and epidemiological studies of past populations.

Human skeletal remains analysis rom pallemalala shell midden in Southern Sri Lanka / Análisis de restos óseos humanos del vertedero de conchas de pallemalala en el sur de Sri Lanka

SUMMARY: Sri Lanka, an island off the tip of the Southeast of the Indian subcontinent processes the earliest skeletal evidence of anatomically modern Homo sapiens (37,000 B.P.) and the best human skeletal record sequence in the South Asian region. Adding another to the list, the skeletal remains, which belong to Mesolithic culture were found at Pallemalala shell midden in Southern Sri Lanka during scientific archaeological exploration by Postgraduate Institute of Archaeology, University of Kelaniya. Sri Lanka. The aim of the study was to determine the minimum number of human individuals, age, sex, and pathological conditions related to the ancient Pallemalala commiunity. For the primary analysis, 426 bone fragments were available. Out of those, 233 bones were identified as human bones which represent 7 minimum number of individuals. The rest of the collection comprises some animal bones and shell species. The community was predominated by the female population. The identified age categories were around 20 years, between 35-45 years, and over 45 years. The encountered pathological lesions were bone thickening, alveolar resorption, dental abscesses, dental caries, antemorterm tooth loss, calculus deposits and brown colour stains on teeth. Regarding the dietary pattern, it was evident that their diet may have consisted of coarse foodstuffs with an extremely basic dietary chemistry.

RESUMEN: Sri Lanka es una isla en la punta del sureste del subcontinente indio que procesa la evidencia esquelética más antigua del Homo sapiens anatómicamente moderno (37.000 AP) y la mejor secuencia de registros esqueléticos humanos en la región del sur de Asia. Agregando otro elemento a la lista, los restos óseos, que pertenecen a la cultura mesolítica, se encontraron en el vertedero de conchas de Pallemalala en el sur de Sri Lanka durante la exploración arqueológica científica realizada por el Instituto de Postgrado de Arqueología de la Universidad de Kelaniya, Sri Lanka. El objetivo del estudio fue determinar el número mínimo de individuos humanos, edad, sexo y condiciones patológicas relacionadas con la antigua comunidad Pallemalala. Para el análisis se dispuso de 426 fragmentos óseos. De esos, 233 huesos fueron identificados como huesos humanos que representan un número mínimo de 7 individuos. El resto de la colección se componía de algunos huesos de animales y especies de conchas. La comunidad estaba dominada por la población femenina. Las categorías de edad identificadas fueron alrededor de 20 años, entre 35-45 años y mayores de 45 años. Las lesiones patológicas encontradas fueron engrosamiento óseo, reabsorción alveolar, abscesos dentales, caries dental, pérdida de dientes antemortem, depósitos de cálculo y manchas de color marrón en los dientes. En cuanto al patrón dietético, era evidente que su dieta pudo haber consistido en alimentos toscos con una química dietética extremadamente básica.

Are cranial peri-mortem fractures identifiable in cremated remains? A study on 38 known cases.

Accurate identification and analysis of signs of trauma on human bone is one of the mainstays of forensic pathology. However, when a forensic pathologist has to deal with charred remains, the task become extremely difficult, because tissues are subjected to severe morphological alterations and their assessment can be critically distorted. We analyzed 38 individuals with peri-mortem skull fractures due to falls from height (17 cases), traffic accidents (16 cases), gunshots wounds (5 cases), of which we had the demographic and clinical data and the autopsy report with the description and photographic records of the fracture lines. After autopsy, the bodies were cremated in gas furnaces and the analysis of cremated cranial remains was conducted in order to verify if it was possible to reconstruct the original peri-mortem fractures and verify differences between known peri-mortem and post-mortem fractures. After 90 min and exposure to temperatures up to 1280 °C, in less than a third of cases (11-29%) the original peri-mortem fracture pattern could be found and reconstructed. The edges and the surface of the fractures can preserve their proper morphology, or they can be affected by post-mortem heat-induced fractures and deformations. Interestingly whenever peri-mortem fracture margins showed the evidence of yellow/brownish colouration, a matte appearance was observed, much different from post-mortem fractures, which may provide further food for thought for the identification of peri-mortem fractures after the cremation process.

Macroscopic determination of the pre-burning condition of human remains recovered from an unusual forensic context: A case report.

Understanding the taphonomic process of burning allows forensic practitioners to better interpret remains and reconstruct the burning incident in a medicolegal investigation. This can yield critical information, especially when other details, such as the biological profile, are obscured by fire. A forensic case involving human remains recovered within a Greek Orthodox cemetery that exhibited a distinctive pattern of differential burning is reported. The bones exhibited a range of differential burning in a pattern inconsistent with the anatomical position of the remains during heat exposure. No macroscopic signs of skeletal trauma or evidence of dismemberment were present on the remains. The pre-burning condition of the bones was inferred based on the presence of the differential burning and its localization identified through analysis of a suite of macroscopic heat-induced traits, as well as consideration of the forensic context. The anthropologists concluded that the bones were skeletonized prior to burning and that the remains were once interred or stored in an ossuary and were likely burned in a ritualistic manner, given the artifacts present and the conspicuous location of the burn area. This case highlights the practical experience required of forensic practitioners encountering burned remains and the necessity of understanding fire as a taphonomic agent that can be analyzed to determine pre-burning condition and the scenario of the burning event in a medicolegal investigation. Furthermore, this case underscores the achievability of pre-burning condition determination using macroscopic traits alone, even when there is a paucity of skeletal elements in the inventory. Due to knowledge of the funerary customs of modern-day Greece, the practitioners were able to interpret the origin of the remains, thereby this paper provides insight into a geographically specific forensic context.

Common and much less common scenarios in which botany is crucial for forensic pathologist and anthropologists: a series of eight case studies.

It is commonly accepted that crime scene recovery and recording are key moments of any judicial inspection in which investigators must decide on the correct strategies to put into place. Complex outdoor scenarios, presenting partially or entirely skeletonised remains, can benefit more than others by the intervention of environmental specialists (forensic anthropologists, archaeologists, entomologists and botanists). These experts are capable of singling out, correctly recording and recovering environmental evidence that can lead to a more comprehensive reconstruction of a given criminal episode. If human remains are discovered in an outdoor scenario, the on-site presence of a botanist will guarantee a correct approach to the identification, recording and recovery of any botanical evidence. If an on-site botanist is not available, the operators must be capable of both the botanical evaluation of a scene and the implementation of correct botanical sampling protocols.The following collection of unusual case histories that aim at underlining the efficacy of forensic botany will examine the determination of post mortem or the post depositional interval, evidence for a victim's post mortem transfer, evidence for the identification of a primary crime scene and evidence for the identification of a victim's dismemberment site. In another two cases, one, we will illustrate the important role that forensic botany played in the discrimination between botanical material used to voluntarily conceal a victim and vegetation that had grown naturally above a disposal site, whereas the other will highlight the protocols implemented for the identification of a murder weapon.

Could a "body fragmentation index" be useful in reconstructing events prior to burial: Case studies of selected primary and secondary mass graves from eastern Bosnia.

This study analyses variable levels of body fragmentation among secondary mass grave sites with similar formation process history. The study is based on data from 10 commingled secondary mass grave sites and two primary sites related to the war in Bosnia in 1995. The aim was to investigate differences in level of body fragmentation between mass graves of similar origin and taphonomy. In order to quantify the degree of fragmentation (and level of commingling) within a grave, we introduced a fragmentation index (FI). FI represents the ratio between the number of complete bodies and number of body parts from the same context. Results show high discrepancies in body fragmentations between different sites. FI for secondary sites of similar formation history varied from 0.01 to 0.59 (max = 1), while two primary sites have values 0.92 and 0.90 respectively. Variable levels of fragmentation among similar secondary sites suggest a possibility of different peri mortem circumstances of buried, so we tested whether the "body fragmentation index" could assist in elucidating the manner of death. Unusually high levels of body fragmentation (FI value below 0.1) in some secondary sites may indicate that body disarticulation was most likely caused peri-mortem by explosives, land mines, mortars or tank fire, all suggesting a combat situation.

A field study to evaluate PMI estimation methods for advanced decomposition stages.

Estimating the postmortem interval (PMI) is one of the major tasks and a continuous challenge in forensic pathology. It is often an exclusion process of available methods, which ultimately can lead to an unsatisfactory outcome due to poor reliability. This problem is most acute in the late PMI, when decomposition proceeds and some methods (such as rigor, livor, and algor mortis) are no longer applicable. Several methods, such as forensic entomology, skeletal muscle protein degradation, and the study of body decomposition by application of a morphological scoring, are expected to provide further information; however, all have certain limitations and weaknesses. Availability of a tool-box of methods allows a case-specific selection of the most appropriate one(s), or eventually provides improvements in the overall accuracy and precision of the PMI estimation by merging and combining methods. To investigate practical (field) application, eventual interferences, and/or synergetic effects, as well as the robustness of these methods towards specific influencing factors, a field study was conducted, using eight pig cadavers of different body weights and physical coverage, left to decompose under natural conditions for 16 days. Morphological changes during decomposition were assessed using the total body score (TBS), muscle samples were collected to analyze protein degradation, and insect colonization was evaluated. The results reveal strengths and current limitations of all tested methods, as well as promising synergistic effects, and thus, provide a baseline for targeted future research.

Skull fractures in forensic putrefied/skeletonised cases: The challenge of estimating the post-traumatic interval.

The objective of our study was to assess the reliability of the estimation of posttraumatic survival time (PTST) in forensic cases based on microCT and histology of putrefied/dry bone samples with comparison of initial macroscopic fracture classification performed during autopsy. Macroscopic morphological patterns of bone fracture are routinely used in forensic pathology and anthropology to distinguish between antemortem, perimortem and postmortem injuries. Based on macroscopic and microscopic analysis of six craniofacial fractures, our study results illustrate the need to complete macroscopical findings and initial fracture classification with microscopic analysis to avoid any inaccuracy. MicroCT has become a powerful technique to identify early bone healing signs but histology remains the gold standard to estimate the PTST and determine vital fracture based on hemorrhage marker. Raman microspectroscopy can identify a blood clot in the fracture line.

The use of torus palatinus in the identification of unknown skeletal remains.

Torus palatinus (TP) is a benign, bony outgrowth located on the hard palate. Variation in this trait occurs, ranging from absent to a protuberance several millimeters in length. If a TP becomes too large, it can interfere with daily activities and thus warrant medical attention. This case utilised the unique morphology of a TP after surgical invention to identify the unknown skeletal remains of a woman missing for six years. Even though there is still much unknown regarding the aetiology, occurrence and prevalence of TP, this study demonstrates the usefulness of anatomical variants in the identification of unknown skeletal remains.

The synergy between radiographic and macroscopic observation of skeletal lesions on dry bone.

The diagnosis of bone lesions is a fundamental part of the study of skeletal remains, both in the archeological and forensic context. On the one side, the literature proved the relevance of radiography for the detection of bone lesions; on the other side, the careful macroscopic observation of the morphology of bone lesions is often underestimated. For this study, we examined and performed plain radiography on 14 skeletons of the CAL Milano Cemetery Skeletal Collection diagnosed with rheumatoid arthritis, diabetes, multiple myeloma, metastatic cancer, and osteomalacia to compare the macroscopic morphology and radiographic visualization of bone lesions. At least 200 osteolytic lesions and 65 areas of proliferative bone reaction (either spongiosclerotic or periosteal) were studied. We realized "comparative sets" of macroscopic pictures and radiographic imaging of the same skeletal elements to allow comparisons of detection and recognition of bone lesions. As a result, while trabecular lesions may be lost through naked eye observation, many lesions can also be unperceived on radiographs due to contrast, including periosteal reactions, osteolytic lesions, and spongiosclerosis. The aim of this research was to investigate the strengths and pitfalls of digital radiography and macroscopic analysis and to demonstrate the synergy of a complementary approach between the two methods for lesion analysis in dry bone.

Multiple osteomata from medieval Tuscany, Italy (ca. 10th-12th AD).

OBJECTIVE: To explore the possible etiology of multiple osteomata on a skull and long bones from an individual from a medieval site in Tuscany, Italy. MATERIALS: Human skeletal remains dating to the 10th-12th century AD from the parish church of S. Pietro in Pava, in the province of Siena (Tuscany, Central Italy). METHODS: Macroscopic and imaging analyses (Cone Beam Computed Tomography). RESULTS: Nine round-shaped new bone formations are observed on a female individual aged 40-50 years. The lesions have a smooth surface and range from 2.2-6 mm in diameter. CONCLUSIONS: Cone Beam Computed Tomography confirmed that the lesions were composed of compact bone. Macroscopic and radiological features suggest the presence of nonsyndromic multiple osteomata. SIGNIFICANCE: Single cranial osteomata are commonly observed in osteoarchaeological remains, but multiple osteomata are rare and might assist in our understanding of neoplastic conditions in the past. LIMITATIONS: The lack of soft tissues prevents the diagnosis of complex disorders, such as the Gardner syndrome, which is characterised by multiple osteomata and polyposis of the colon. SUGGESTIONS FOR FURTHER RESEARCH: Careful investigation and reporting of all neoplastic lesions in ancient human remains in order to increase our knowledge about the etiology in past human populations.

Using evidence from hair and other soft tissues to infer the need for and receipt of health-related care provision.

The Bioarchaeology of Care approach developed by Tilley is usually applied to skeletalized human remains, given the usual constraints of preservation bias that are seen with archaeological assemblages. However, other tissues, such as hair are sometimes preserved and can provide a wealth of information that can supplement the skeletal data. Archaeological hair has been analysed for drug compounds for almost thirty years. This article integrates data from hair analyses for coca metabolites, stable light isotope analysis and aDNA to expand the potential of the Bioarchaeology of Care approach using the example of a spontaneously mummified adult female from northern Chile.

New Approaches to Juvenile Age Estimation in Forensics: Application of Transition Analysis via the Shackelford et al. Method to a Diverse Modern Subadult Sample.

Dental development is one of the most widely utilized and accurate methods available for estimating age in subadult skeletal remains. The timing of tooth growth and development is regulated by genetics and less affected by external factors, allowing reliable estimates of chronological age. Traditional methodology focuses on comparing tooth developmental scores to corresponding age charts. Using the Moorrees, Fanning, and Hunt (MFH) developmental scores, Shackelford and colleagues embed the dental development method in a statistical framework based on transition analysis. They generated numerical parameters underlining each "stage" and age-at-death distribution and applied them to fossil hominins and Neanderthals with limited application to modern humans. We use this same method on a subadult test sample (n = 201), representing modern individuals that may become part of the forensic record. We assess the probability coverage of the Shackelford et al. method derived from MFH standards as it applies to all available dentition. Results indicate promise: the age range at 90% and 95% confidence levels includes the chronological age of almost every individual tested. The maximum likelihood age estimates underestimate age by 0.5-2.5 years for individuals 0-15 years of age and by >2.5 years for individuals 16-18 years of age, as previously shown. In an attempt to refine the method, we adjusted the numerical parameters underlying the stages for developing teeth based on a combined modern reference sample (n = 1,964) and tested these revised parameters using the same test sample. The estimated ages from the modified method differ from the original Shackelford et al. methodology by underestimating age to a lesser degree. The modified method does include mean age-at-attainment values for earlier stages of several teeth, allowing for the calculation of narrower confidence intervals. While this study highlights areas of future research in refining dental developmental aging by transition analysis, it also demonstrates that the Shackelford et al. method is applicable and accurate when aging modern subadults in forensic work.

Survey and Insights into Unmanned Aerial-Vehicle-Based Detection and Documentation of Clandestine Graves and Human Remains.

Numerous biological and archaeological studies have demonstrated the legitimacy of remote sensing in anthropology. This article focuses on detecting and documenting terrestrial clandestine graves and surface remains (CGSR) of humans using unmanned aerial vehicles (UAVs), sensors, and automatic processing algorithms. CGSR is a problem of complex decision making under uncertainty that requires the identification and intelligent reasoning about direct evidence of human remains and their environmental fingerprints. As such, it is as much an engineering and geospatial problem as it is an anthropology problem. This article is an effort to survey existing work across disciplines and to provide insights and recommendations to assist future research. To support our claims, preliminary experiments were performed at the Forensic Anthropological Research Facility at Texas State University using UAVs, hyperspectral imaging, thermal imaging, and structure from motion. Prior work, our experience, and preliminary results indicate that both great potential and extreme challenges face remote sensing of CGSR.

Metastatic Prostate Carcinoma from Imperial Rome (1st to 2nd Centuries AD).

OBJECTIVE: This study describes and discusses a rare case of metastatic carcinoma that affected the skeleton of an adult male recovered in the necropolis of Casal Bertone in Rome (Italy). The necropolis, which dates back to the Imperial Age (1st to 2nd century AD), is located near some residential structures and a large place identified as a fullery (fullonica). METHODS: Anthropological and paleopathological studies of the skeletal remains were performed via careful macroscopic, microscopic, radiological (X-ray and CT scan), and histological investigation. RESULTS: The skeleton displayed mixed osteoclastic and osteoblastic lesions that mainly involved the axial bones, in particular the sternum, the ribs, the spine, and the scapular and pelvic girdles. The anatomical distribution and the destructive and proliferative nature of the lesions suggested diffuse metastases arising from a soft-tissue primary cancer. The age and sex of the individual, as well as radiographic and histological pictures, allowed diagnosis of an advanced prostate cancer with extensively diffused bone metastases. CONCLUSION: At present, this is the only case of prostate cancer from the Imperial Age recovered in Rome.

Altered shapes, same people: Scaphocephaly in the early modern Bucharest.

Premature sagittal suture synostosis was identified in eight specimens dated between the 18th and 20th centuries CE, of which seven are kept within the Rainer Osteological Collection in Bucharest and one was retrieved from the archaeologically investigated Saint Sava Church Cemetery. Our study focused on metric measurements, aiming to compare these specimens with three other samples comprising normal and scaphocephalic individuals, with both a Romanian and worldwide distribution. In total, 374 individuals were used in this study. However, although metric measurements clearly show that these individuals were different in their appearance, the socio-cultural data point to them being normal members of the society, as suggested by the funerary features of the archaeological specimens and their position within the Rainer Osteological Collection.

Elongated odontoid process in late Holocene skeletal remains from B6 archaeological site, Mendoza, Argentina.

The odontoid process of the axis can be affected by congenital or acquired pathologies. While abnormalities such as os odontoideum, agenesis, and fractures are reported in archaeological remains, the abnormality of an elongated length of the odontoid process has not been described in the paleopathological literature. The aim of this paper is to evaluate two individuals with elongated odontoid processes from a skeletal assemblage from the B6 archaeological site (Mendoza, Argentina), and to discuss the possible etiologies of the condition, with particular attention given to the relation to trauma and Crowned Dens Syndrome (CDS), a condition characterized by the ossification of ligaments of the odontoid process of the second cervical vertebra.

A juvenile with compromised osteogenesis provides insights into past hunter-gatherer lives.

The Late Archaic in northeastern North America (4500-2800 B.P.) pre-dates reliance on pottery and domesticated plants. It is thought to reflect a highly mobile, seasonal migratory foraging/hunting regimen. A juvenile skeleton with pervasive bone wasting and fragile jaws from the Hind Site (AdHk-1), ca. 3000 B.P., southwestern Ontario, provides evidence of the social context of her family group, including aspects of mobility and food management. The well-preserved bones and teeth are considered in bioarchaeological context. Radiographic, osteometric and cross-sectional geometric approaches to assessing musculoskeletal function are presented, plus differential diagnosis of the bone wasting condition. All bones of the probable female (aged approx. 16yr) show stunting and wasting. Wedged lower vertebral bodies, porous trabeculae, undeveloped bicondylar angles (femur) and abnormally low cortical long bone mass are consistent with chronically reduced ambulation. Few teeth remain in the dramatically resorbed alveoli; slight tooth wear and substantial calculus suggest a modified (soft) diet. Osteogenesis imperfecta type IV is the most probable etiology. The extended survival of this juvenile who may never have walked reflects collective care. The case provides evidence of a past lifeway that appears to have been organized around logistic mobility, including occupational stability and food storage.