RESUMO
BACKGROUND: Schistosomiasis is a parasitic disease that is transmitted by skin contact with waterborne schistosome cercariae. Mass drug administration with praziquantel is an effective control method, but it cannot prevent reinfection if contact with cercariae infested water continues. Providing safe water for contact activities such as laundry and bathing can help to reduce transmission. In this study we examine the direct effect of UV light on Schistosoma mansoni cercariae using ultraviolet light-emitting diodes (UV LEDs) and a low-pressure (LP) mercury arc discharge lamp. METHODOLOGY: S. mansoni cercariae were exposed to UV light at four peak wavelengths: 255 nm, 265 nm, 285 nm (UV LEDs), and 253.7 nm (LP lamp) using bench scale collimated beam apparatus. The UV fluence ranged from 0-300 mJ/cm2 at each wavelength. Cercariae were studied under a stereo-microscope at 0, 60, and 180 minutes post-exposure and the viability of cercariae was determined by assessing their motility and morphology. CONCLUSION: Very high UV fluences were required to kill S. mansoni cercariae, when compared to most other waterborne pathogens. At 265 nm a fluence of 247 mJ/cm2 (95% confidence interval (CI): 234-261 mJ/cm2) was required to achieve a 1-log10 reduction at 0 minutes post-exposure. Cercariae were visibly damaged at lower fluences, and the log reduction increased with time post-exposure at all wavelengths. Fluences of 127 mJ/cm2 (95% CI: 111-146 mJ/cm2) and 99 mJ/cm2 (95% CI: 85-113 mJ/cm2) were required to achieve a 1-log10 reduction at 60 and 180 minutes post-exposure at 265 nm. At 0 minutes post-exposure 285 nm was slightly less effective, but there was no statistical difference between 265 nm and 285 nm after 60 minutes. The least effective wavelengths were 255 nm and 253.7 nm. Due to the high fluences required, UV disinfection is unlikely to be an energy- or cost-efficient water treatment method against schistosome cercariae when compared to other methods such as chlorination, unless it can be demonstrated that UV-damaged cercariae are non-infective using alternative assay methods or there are improvements in UV LED technology.
Assuntos
Desinfecção/métodos , Schistosoma mansoni/efeitos da radiação , Raios Ultravioleta , Purificação da Água/métodos , Água/parasitologia , Animais , Cercárias/efeitos da radiação , HumanosRESUMO
Baboons vaccinated with radiation-attenuated cercariae develop high levels of protection against schistosome infection, correlating to high antibody titres towards schistosome antigens with unknown molecular identity. Using a microarray consisting of glycans isolated from different life-stages of schistosomes, we studied the anti-glycan immunoglobulin (Ig) G and IgM responses in vaccinated and challenged baboons over a time course of 25 weeks. Anti-glycan IgM responses developed early after vaccination, but did not rise in response to later vaccinations. In contrast, anti-glycan IgG developed more slowly, but was boosted by all five subsequent vaccinations. High IgM and IgG levels against O-glycans and glycosphingolipid glycans of cercariae were observed. At the time of challenge, while most antibody levels decreased in the absence of vaccination, IgG towards a subset of glycans containing multiple-fucosylated motifs remained high until 6 weeks post-challenge during challenge parasite elimination, suggesting a possible role of this IgG in protection.
Assuntos
Anticorpos Anti-Helmínticos/imunologia , Papio anubis/imunologia , Papio anubis/parasitologia , Schistosoma mansoni/efeitos da radiação , Esquistossomose mansoni/veterinária , Animais , Antígenos de Helmintos/imunologia , Cercárias/imunologia , Cercárias/efeitos da radiação , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Análise em Microsséries/métodos , Polissacarídeos/imunologia , Polissacarídeos/metabolismo , Schistosoma mansoni/imunologia , Esquistossomose mansoni/imunologia , Esquistossomose mansoni/prevenção & controle , VacinaçãoRESUMO
Schistosoma mansoni cercariae display specific behavioral responses to abiotic/biotic stimuli enabling them to locate and infect the definitive human host. Here we report the effect of such stimulants on signaling pathways of cercariae in relation to host finding and invasion. Cercariae exposed to various light/temperature regimens displayed modulated protein kinase C (PKC), extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38 MAPK) activities, with distinct responses at 37 °C and intense light/dark, when compared to 24 °C under normal light. Kinase activities were localized to regions including the oral sensory papillae, acetabular ducts, tegument, acetabular glands, and nervous system. Furthermore, linoleic acid modulated PKC and ERK activities concurrent with the temporal release of acetabular gland components. Attenuation of PKC, ERK, and p38 MAPK activities significantly reduced gland component release, particularly in response to linoleic acid, demonstrating the importance of these signaling pathways to host penetration mechanisms.
Assuntos
Cercárias , Sistema de Sinalização das MAP Quinases , Proteínas Quinases/metabolismo , Schistosoma mansoni , Animais , Cercárias/efeitos dos fármacos , Cercárias/enzimologia , Cercárias/metabolismo , Cercárias/efeitos da radiação , Humanos , Ácido Linoleico/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/fisiologia , Sistema de Sinalização das MAP Quinases/efeitos da radiação , Fosforilação/efeitos dos fármacos , Fosforilação/efeitos da radiação , Schistosoma mansoni/efeitos dos fármacos , Schistosoma mansoni/enzimologia , Schistosoma mansoni/metabolismo , Schistosoma mansoni/efeitos da radiaçãoRESUMO
Although the current treatment of schistosomiasis relies largely on praziquantel (PZQ), it has not been successful in significantly reducing the overall rate of disease cases, one of the suggested reasons being the inevitable resistance to PZQ. Previous studies showed that radiation-attenuated vaccine provides protection against Schistosoma mansoni in a host of various species. In the present study, we evaluated the effect of various vaccination strategies in C57BL/6 mice, including single or multiple vaccination strategy, subcurative dose (20 mg/kg) of PZQ, and a combination of single vaccination with subcurative dose of PZQ. Treatment either with subcurative dose of PZQ or with a single vaccination of attenuated cercariae (500 per mouse), caused significant reduction in total worm burden, hepatic, and intestinal ova counts of 43.03, 73.2, and 59.5 and 37.97, 52.02, and 26.3%, respectively. Furthermore, tegumental changes were observed. In multiple vaccinated group, there was an extensive lysis in tegumental layers. High deformations in gastrodermis, testis cells, vitelline cells, and oocytes were recorded. Also, this study is to explore the role of humoral immunity using highly resistant rabbits that had been exposed to three immunizations with ultraviolet (UV)-irradiated cercariae (8000 per rabbit in each immunization), and their sera were tested for their ability to transfer protection. The reduction in challenge worm burden had reached 32.76-43.64% when compared with recipients of normal serum or no serum. The reduction in hepatic and intestinal ova counts reached to 74.4 and 71.08% in group immunized with vaccinated rabbit sera. Swelling and extensive lysis of tegumental layers, gastrodermis lumen, spermatocytes, and deformation of oocytes were recorded with more severity than that recorded in normal rabbit sera group. Our findings recorded that multiple vaccination strategy is the most effective strategy then passive transfer of vaccinated rabbit. This gives guiding in the design the appropriate therapeutic strategy.
Assuntos
Praziquantel/farmacologia , Schistosoma mansoni/ultraestrutura , Esquistossomose mansoni/parasitologia , Animais , Feminino , Humanos , Imunização Passiva , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Eletrônica de Transmissão , Coelhos , Schistosoma mansoni/efeitos dos fármacos , Schistosoma mansoni/isolamento & purificação , Schistosoma mansoni/efeitos da radiação , Esquistossomose mansoni/tratamento farmacológico , Esquistossomose mansoni/prevenção & controle , Raios Ultravioleta , Vacinação , Vacinas Atenuadas/administração & dosagemRESUMO
Although the lifecycle of Schistosoma spp. and pathophysiology of schistosomiasis have been established, the mechanism by which cercariae find their host is not well understood. Speculatively, host infection by random and accidental host contact is not as biologically plausible as a biochemical mechanism of mammalian attraction. A few studies have indicated that biochemical cues and temperature gradients may play a role in host identification, attraction and attachment triggers. This study aimed to elucidate these mechanisms more specifically through evaluation of biochemical, age and temperature influences leading to Schistosoma mansoni cercariae attraction and attachment behaviors. Oleic acid, a common unsaturated free fatty acid in the outer layer of human skin, was tested for cercariae attraction across biologically relevant concentrations. Influence of media type (beeswax, nail varnish and agar), age-dependent behavior variability and environmentally appropriate temperatures (22 and 30 °C) were also evaluated. Results indicated that oleic acid at concentrations of 0.3, 0.9 and 1.8 g/mL in beeswax significantly increased median attachment to media (median attachment of 7.50%, 4.20% and 3.71%, respectively, P<0.001), compared with plain beeswax, with maximal attachment of 30.30% at 0.3g/mL of oleic acid. In media containing 0.3 g/mL of oleic acid, cercarial attachment was highest for freshly emerged cercariae to 5h post-emergence, with a significant decrease in attachment behavior at 10h post-emergence (P<0.01). Aquatic temperature at which cercariae were exposed to media did not yield significant results (P value >0.05). Biochemical, age and environmental factors influencing cercarial host attraction and attachment behavior have been elucidated by this study. This information will inform further development of devices for environmental surveillance and potentially improve cercarial exposure prevention strategies.
Assuntos
Fatores Quimiotáticos/metabolismo , Interações Hospedeiro-Patógeno , Ácido Oleico/metabolismo , Schistosoma mansoni/fisiologia , Animais , Schistosoma mansoni/efeitos dos fármacos , Schistosoma mansoni/efeitos da radiação , TemperaturaRESUMO
Schistosomes develop successfully in susceptible snails but are encapsulated and killed in resistant ones. Mechanism(s) shaping these outcomes involves the parasites ability to evade the snail's defenses. RNA analysis from resistant (BS-90), non-susceptible (LAC2) and susceptible (NMRI) juvenile Biomphalaria glabrata to Schistosoma mansoni revealed that stress-related genes, heat shock protein 70 (Hsp 70) and reverse transcriptase (RT), were dramatically co-induced early in susceptible snails, but not in resistant/non-susceptible ones. These transcripts were, however, down regulated upon exposure to irradiated parasites although penetration behavior of irradiated vs. normal parasites were the same, indicating that Hsp 70 and RT regulation was elicited by infection and not injury. Understanding molecular events involved in stress response transcriptional regulation of Hsp 70 in juvenile snails could pave a way towards the identification of genes involved in schistosome/snail interactions.
Assuntos
Biomphalaria/imunologia , Biomphalaria/parasitologia , Proteínas de Choque Térmico HSP70/biossíntese , DNA Polimerase Dirigida por RNA/biossíntese , Schistosoma mansoni/fisiologia , Animais , Biomphalaria/genética , Regulação para Baixo/imunologia , Raios gama , Expressão Gênica , Proteínas de Choque Térmico HSP70/genética , DNA Polimerase Dirigida por RNA/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Schistosoma mansoni/imunologia , Schistosoma mansoni/efeitos da radiação , Ativação Transcricional/imunologiaRESUMO
Ultraviolet B (UVB, 280-315nm) radiation is detrimental to both of larvae of the digenetic trematode Schistosoma mansoni and its snail intermediate host, Biomphalaria glabrata. We explored effects of UVB on three aspects of the interaction between host and parasite: survival of infected snails, innate susceptibility and resistance of snails to infection, and acquired resistance induced by irradiated miracidia. Snails infected for 1 week showed significantly lower survival than uninfected snails following irradiation with a range of UVB intensities. In contrast to known immunomodulatory effects in vertebrates, an effect of UVB on susceptibility or resistance of snails to infection could not be conclusively demonstrated. Finally, exposure of susceptible snails to UVB-irradiated miracidia failed to induce resistance to a subsequent challenge with nonirradiated miracidia, a result similar to that reported previously with ionizing radiation.
Assuntos
Biomphalaria/parasitologia , Interações Hospedeiro-Parasita/efeitos da radiação , Schistosoma mansoni/fisiologia , Raios Ultravioleta , Animais , Biomphalaria/efeitos da radiação , Imunidade Inata/efeitos da radiação , Schistosoma mansoni/efeitos da radiaçãoAssuntos
Interações Hospedeiro-Parasita , Schistosoma mansoni/fisiologia , Esquistossomose mansoni/parasitologia , Animais , Regulação da Expressão Gênica/efeitos da radiação , Humanos , Larva/genética , Larva/crescimento & desenvolvimento , Larva/fisiologia , Larva/efeitos da radiação , Schistosoma mansoni/genética , Schistosoma mansoni/crescimento & desenvolvimento , Schistosoma mansoni/efeitos da radiação , Esquistossomose mansoni/imunologia , Esquistossomose mansoni/veterinária , Pele/imunologia , Pele/parasitologia , CaramujosRESUMO
The swimming behaviour of many cercarial species is governed by sensitive responses to light and dark stimuli. We studied the effect of irradiance changes on swimming behaviour of Schistosoma mansoni cercariae and found only insignificant responses. Decreasing light intensity results in a weak tendency of the cercariae to start swimming movements, and increasing light intensity tends to inhibit the start of swimming. These responses seem not suitable to increase the transmission success. Whether the cercariae show chemo-orientation towards human skin was studied by video-tracking their swimming movements around agar containing human-skin-surface extracts and when immersed into skin extracts. They showed no directed chemotactic orientation, as they did not correct their swimming paths in direction towards the skin-extract substrates, also not when shifting between forward and backward swimming. However, the cercariae shifted more between backward and forward swimming and therewith increased their rate of change of direction. This response may support an accumulation around the skin substrates and could guide the cercariae towards the host's skin surface when they are already in close proximity to it.
Assuntos
Schistosoma mansoni/fisiologia , Schistosoma mansoni/efeitos da radiação , Pele , Natação/fisiologia , Animais , Quimiotaxia , Escuridão , Interações Hospedeiro-Parasita , Humanos , Luz , Camundongos , Schistosoma mansoni/crescimento & desenvolvimento , Pele/química , Pele/parasitologiaRESUMO
The absence of appropriate animal models is a challenge for those designing anti-schistosome vaccines. In order to observe the development of hepatic granulomata, 20 Krad irradiated cercaria vaccinated grivet monkeys (Cercopithecus aethiops aethiops) were experimentally infected with Schistosoma mansoni. Most human clinical manifestations of schistosomiasis were noted in the infected monkeys. Intraperitonial immunization with 20 Krad irradiated cercaria resulted in a relatively less granulomatous reaction and lower egg mass (P<0.05) than in the controls. The mean total adult worms and egg production was also lower in the vaccinated group. This study underlines the significant role of irradiated cercariae vaccination on the establishment of female adults and consequent reduction of pathology in the grivet Cercopithecus aethiops aethiops.
Assuntos
Granuloma/patologia , Fígado/patologia , Schistosoma mansoni/imunologia , Esquistossomose mansoni/patologia , Vacinas , Animais , Biomphalaria , Chlorocebus aethiops , Modelos Animais de Doenças , Fezes/parasitologia , Feminino , Raios gama , Granuloma/prevenção & controle , Fígado/parasitologia , Masculino , Contagem de Ovos de Parasitas , Schistosoma mansoni/efeitos da radiação , Esquistossomose mansoni/prevenção & controle , Vacinação/normas , Vacinas/imunologia , Vacinas/normasRESUMO
The cytokine interplay during the development of protective immunity to the radiation-attenuated (RA) schistosome vaccine has been extensively characterized over recent years, yet the role of costimulatory molecules in the development of cell-mediated immunity is much less well understood. Here we demonstrate the importance of CD40/CD154 in vaccine-induced immunity, as CD154(-/-) mice exposed to RA schistosomes develop no protection to challenge infection. We showed that vaccinated CD154(-/-) mice have defective Th1-associated immune responses in the skin-draining lymph nodes and the lungs, with reduced or absent levels of interleukin-12p40 (IL-12p40), gamma interferon, and nitric oxide, but elevated levels of lung IL-4 and IL-5. The expression of major histocompatibility complex II (MHC-II) on antigen-presenting cells recovered from the lungs of vaccinated CD154(-/-) mice was also severely compromised. The administration of anti-CD40 monoclonal antibody (MAb) to CD154(-/-) mice did not reconstitute sustained Th1 responses in the lymph nodes or the lungs, nor did the MAb restore anti-parasite immunoglobulin G production or protective immunity. On the other hand, the administration of recombinant IL-12 (rIL-12) to CD154(-/-) mice shortly after vaccination caused elevated and sustained levels of Th1-associated cytokines, rescued MHC-II expression by lung CD11c(+) cells, and restored the appearance of inflammatory effector foci in the lungs. However, the treatment of CD154(-/-) mice with rIL-12 did not restore protection. We conclude that protective immunity to the RA schistosome vaccine is CD154 dependent but is independent of IL-12-orchestrated cellular immune mechanisms in the lungs.
Assuntos
Ligante de CD40/metabolismo , Interleucina-12/administração & dosagem , Schistosoma mansoni/imunologia , Esquistossomose mansoni/imunologia , Esquistossomose mansoni/prevenção & controle , Vacinas Atenuadas/administração & dosagem , Animais , Anticorpos Anti-Helmínticos/sangue , Ligante de CD40/deficiência , Ligante de CD40/genética , Feminino , Interleucina-12/imunologia , Pulmão/imunologia , Pulmão/parasitologia , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/imunologia , Schistosoma mansoni/crescimento & desenvolvimento , Schistosoma mansoni/patogenicidade , Schistosoma mansoni/efeitos da radiação , Esquistossomose mansoni/parasitologia , Células Th1/imunologia , Vacinação , Vacinas Atenuadas/imunologia , Vacinas Atenuadas/efeitos da radiaçãoRESUMO
Schistosoma mansoni occurs in tropical regions where levels of ultraviolet B (UVB; 290-320 nm) light are elevated. However, the effects of UVB on parasite transmission are unknown. This study examines effects of UVB on the miracidia and sporocysts of S. mansoni, focusing specifically on intramolluscan development, infectivity, and the ability to photoreactivate (repair DNA damage using visible light). Histology revealed that miracidia irradiated with 861 J x m(-2) underwent abnormal development after penetrating Biomphalaria glabrata snails. Total number of sporocysts in snail tissues decreased as a function of time postinfection (PI), among both nonirradiated and irradiated parasites; however, this decrease was greater in the latter. Moreover, whereas the proportion alive of nonirradiated sporocysts increased PI, that of irradiated sporocysts, i.e., derived from irradiated miracidia, decreased. Irradiation of miracidia with UVB resulted in decreased prevalence of patent infection (defined by presence of daughter sporocysts) in a dose-dependent manner, and no infections occurred at a dose of 861 J x m(-2). Like many aquatic organisms, including the snail host, parasites were able to photoreactivate if exposed to visible light following UVB irradiation, even subsequent to penetrating snails. These photoreactivation results suggest cyclobutane-pyrimidine dimers in DNA as the primary mechanism of UVB damage, and implicate photoreactivation, rather than nucleotide excision, as the main repair process in S. mansoni.
Assuntos
Biomphalaria/parasitologia , Schistosoma mansoni/efeitos da radiação , Raios Ultravioleta , Animais , Reparo do DNA/fisiologia , Reparo do DNA/efeitos da radiação , Relação Dose-Resposta à Radiação , Luz , Oocistos/efeitos da radiação , Schistosoma mansoni/genética , Schistosoma mansoni/crescimento & desenvolvimento , Schistosoma mansoni/fisiologiaRESUMO
Appropriate animal models are necessary to better understand the immune response in schistosomiasis. Schistosoma mansoni infection was established using irradiated cercariae in Cercopithecus aethiops aethiops (Grivet monkey) to describe immune responses of the serum cytokines, IL-4, IL-10, IL-12, IFN- gamma, and TNF-alpha. Intraperitoneal irradiated cercariae immunization on three occasions resulted in some differences of cytokine production. In primary infection, IL-4 was significantly raised (p=0.03) in the immunized monkeys, and there was an insignificant increase (p>0.05) in IL-10. However, ova excretion did not influence the cytokines, except in the controls where both IL-4 and IL-10 were significantly increased (p<0.05). In the controls, IL-12 and INF-gamma levels were lower after ova excretion, but the inflammatory TNF-alpha increased (p=0.049) and these findings can be associated with more liver pathogenesis in the group. Thus, this work has indicated the potential importance of anti-schistosome vaccine studies on the grivet monkeys.
Assuntos
Chlorocebus aethiops/parasitologia , Citocinas/sangue , Modelos Animais de Doenças , Schistosoma mansoni/imunologia , Esquistossomose mansoni/imunologia , Animais , Ensaio de Imunoadsorção Enzimática , Feminino , Interferon gama/sangue , Interleucina-10/sangue , Interleucina-12/sangue , Interleucina-4/sangue , Masculino , Schistosoma mansoni/efeitos da radiação , Esquistossomose mansoni/prevenção & controle , Fator de Necrose Tumoral alfa/sangue , Vacinas de Produtos Inativados/imunologiaRESUMO
A current or previous schistosome infection might compromise the efficacy of a schistosome vaccine administered to humans. We have therefore investigated the influence of infection on vaccination, using the baboon as the model host and irradiated Schistosoma mansoni cercariae as the vaccine. Protection, determined from worm burdens in test and controls, was not diminished when vaccination was superimposed on a chronic infection, nor was it diminished when it followed a primary infection terminated by chemotherapy. Protection was also assessed indirectly based on fecal egg output and circulating antigen levels, as would be the case in human vaccine trials. In almost all instances, these methods overestimated protection, sometimes with discrepancies of >20%. The overwhelming immune response to egg deposition in infected animals made it difficult to discern a contribution from vaccination. Nevertheless, the well-documented immunomodulation of immune responses that follows egg deposition did not appear to impede the protective mechanisms elicited by vaccination with attenuated cercariae.
Assuntos
Schistosoma mansoni/imunologia , Esquistossomose mansoni/prevenção & controle , Esquistossomose mansoni/parasitologia , Vacinas Atenuadas/imunologia , Vacinas Atenuadas/uso terapêutico , Animais , Anticorpos Anti-Helmínticos/biossíntese , Antígenos de Helmintos/sangue , Antígenos de Helmintos/imunologia , Antígenos de Helmintos/efeitos da radiação , Fezes/parasitologia , Feminino , Raios gama , Humanos , Esquemas de Imunização , Larva/imunologia , Larva/efeitos da radiação , Masculino , Óvulo/imunologia , Papio anubis , Recidiva , Schistosoma mansoni/efeitos da radiação , Esquistossomose mansoni/imunologia , Vacinas Atenuadas/administração & dosagemRESUMO
As a paradigm for the development of a vaccine against human schistosomiasis, the radiation-attenuated (RA) vaccine has enabled the dissection of different immune responses as putative effector mechanisms. This review considers advances made in the past, and updates our knowledge with reference to recent studies that have provided new information relevant particularly to the early innate events after vaccination, and to the nature of the protective effector mechanism. Priming of a protective response by RA larvae is a highly co-ordinated series of events starting in the skin, draining lymph nodes and lungs, leading to the development of various effector responses, ranging from Th1-associated cell-mediated activity, to anti-parasitic antibodies, all of which contribute to the elimination of challenge larvae to varying extents. In this respect, the RA vaccine elicits a multifaceted immune response, from which we can derive valuable insights relevant to the future design of novel delivery systems and adjuvants for recombinant and subunit vaccines.
Assuntos
Schistosoma mansoni/efeitos da radiação , Esquistossomose/imunologia , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/imunologia , Animais , Anticorpos Anti-Helmínticos/sangue , Humanos , Camundongos , Schistosoma mansoni/imunologia , Esquistossomose/parasitologia , Esquistossomose/prevenção & controle , Células Th1/imunologia , VacinaçãoRESUMO
The baboon model of schistosomiasis has been used extensively to study parasite biology, immune responses and pathological manifestations after natural and experimental infections. The body of knowledge accumulated so far has placed this animal model at the pinnacle in the continuing search for new interventions and might hold the key to the development of new anti-schistosome vaccines. In this review paper, we highlight previous and recent studies that have elevated the baboon to be the model of choice for schistosomiasis research. In particular, the long-term studies of re-infection after chemotherapy as well as the interaction between vaccination, chemotherapy and infection are highlighted.
Assuntos
Modelos Animais de Doenças , Schistosoma mansoni/patogenicidade , Esquistossomose mansoni/imunologia , Vacinas Atenuadas/administração & dosagem , Animais , Humanos , Papio , Praziquantel/administração & dosagem , Schistosoma mansoni/efeitos da radiação , Esquistossomose mansoni/tratamento farmacológico , Esquistossomose mansoni/parasitologia , Esquistossomose mansoni/patologia , Esquistossomicidas/administração & dosagem , VacinaçãoRESUMO
Present report demonstrates that repeated radiation of Schistosoma mansoni-infected Biomphalaria glabrata, totaling 15,000 rads, caused a sudden, albeit transient, suppression of cercarial shedding. Initially, sporocysts practically disappeared from the snail tissues. The more resistant developing cercariae presented nuclear clumping and vacuolation, before undergoing lysis. No host tissue reaction was evident at any time. Thirty-four days after the last irradiation, the snails resumed cercarial elimination. By that time numerous sporocysts and developing cercariae were detected, disseminated throughout snail tissues in a pattern similar to that of a highly malignant neoplasm, with no signs of host cellular reactions, which on the other hand were present in non-irradiated infected controls. The region of the ovo-testis was apparently destroyed after radiation, but returned to its normal appearance around 40 days after the last radiation. Ionizing radiation affected both host and parasite in S. mansoni-infected Biomphalaria glabrata, but the resulting impressive changes were soon reversed.
Assuntos
Biomphalaria/parasitologia , Schistosoma mansoni/efeitos da radiação , Animais , Biomphalaria/imunologia , Biomphalaria/efeitos da radiação , Fatores de TempoRESUMO
Radiation-attenuated (RA) schistosomes penetrate the host via the skin where they stimulate intense inflammatory reactions and the release of pro-inflammatory IL-12, important for T(h)1-type immune responses which are partially host protective. However, RA larvae also induce the secretion of regulatory IL-10. We now show that following vaccination of IL-12p40(-/-) mice, abundant IL-10 was produced by in vitro cultured skin biopsies between days 4 and 14, corresponding to the down-regulation of MHC II expression by cells in the dermis and cells that emigrate from the skin. In IL-10(-/-) mice, inflammation of the vaccination site was increased with larger numbers of IL-12p40(+), MHC II(+) and CD86(+) cells in the dermal exudate, and was associated with elevated levels of skin-derived IL-12p40 and IL-1beta. These changes in IL-10(-/-) mice were also reflected by an increased number of cells in the skin-draining lymph nodes (sdLN) and greater levels of lymphocyte proliferation. Moreover, such mice had increased numbers of CD4(+) sdLN cells that were CD25(+), CD28(+) or CD152(+) and accessory cells that were CD40(+) or MHC II(+). Finally, the secretion of IFN-gamma (and IL-12p40) by in vitro cultured sdLN cells was substantially raised in IL-10(-/-) mice, but much reduced in IL-12p40(-/-) mice, resulting in the development of highly polarized T(h)1 and T(h)2 cytokine profiles in the two groups of mice respectively. We conclude that IL-10 has an important role early in the regulation of IL-12-mediated priming of acquired immune responses, and effectively contains excessive dermal inflammation and prevents the development of highly polarized T(h)1-type responses.
Assuntos
Interleucina-10/fisiologia , Interleucina-12/fisiologia , Subunidades Proteicas/fisiologia , Schistosoma mansoni/imunologia , Vacinação , Vacinas Atenuadas/imunologia , Animais , Antígenos/farmacologia , Antígenos CD/metabolismo , Antígeno B7-2 , Antígenos CD4/metabolismo , Linfócitos T CD4-Positivos/metabolismo , Contagem de Células , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Técnicas de Cultura , Orelha Externa/imunologia , Orelha Externa/patologia , Feminino , Citometria de Fluxo , Antígenos de Histocompatibilidade Classe II/metabolismo , Imuno-Histoquímica , Inflamação/imunologia , Injeções Intradérmicas , Interferon gama/metabolismo , Interleucina-1/metabolismo , Interleucina-10/genética , Interleucina-10/imunologia , Interleucina-12/genética , Interleucina-12/imunologia , Subunidade p40 da Interleucina-12 , Interleucina-18/metabolismo , Interleucina-4/metabolismo , Antígenos Comuns de Leucócito/metabolismo , Linfonodos/citologia , Ativação Linfocitária/fisiologia , Glicoproteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Subunidades Proteicas/genética , Subunidades Proteicas/imunologia , Schistosoma mansoni/efeitos da radiação , Pele/química , Pele/imunologia , Pele/patologia , Células Th1/imunologia , Células Th1/fisiologia , Células Th2/imunologia , Células Th2/fisiologiaRESUMO
Radiation-attenuated (RA) schistosome larvae are potent stimulators of innate immune responses at the skin site of exposure (pinna) that are likely to be important factors in the development of Th1-mediated protective immunity. In addition to causing an influx of neutrophils, macrophages, and dendritic cells (DCs) into the dermis, RA larvae induced a cascade of chemokine and cytokine secretion following in vitro culture of pinna biopsy samples. While macrophage inflammatory protein 1alpha and interleukin-1beta (IL-1beta) were produced transiently within the first few days, the Th1-promoting cytokines IL-12 and IL-18 were secreted at high levels until at least day 14. Assay of C3H/HeJ mice confirmed that IL-12 secretion was not due to lipopolysaccharide contaminants binding Toll-like receptor 4. Significantly, IL-12 p40 secretion was sustained in pinnae from vaccinated mice but not in those from nonprotected infected mice. In contrast, IL-10 was produced from both vaccinated and infected mice. This cytokine regulates IL-12-associated dermal inflammation, since in vaccinated IL-10(-/-) mice, pinna thickness was greatly increased concurrent with elevated levels of IL-12 p40. A significant number of IL-12 p40(+) cells were detected as emigrants from in vitro-cultured pinnae, and most were within a population of rare large granular cells that were Ia(+), consistent with their being antigen-presenting cells. Labeling of IL-12(+) cells for CD11c, CD205, CD8alpha, CD11b, and F4/80 indicated that the majority were myeloid DCs, although a proportion were CD11c(-) F4/80(+), suggesting that macrophages were an additional source of IL-12 in the skin.
Assuntos
Antígenos de Diferenciação/análise , Antígeno CD11c/análise , Interleucina-12/metabolismo , Subunidades Proteicas/metabolismo , Schistosoma mansoni/imunologia , Pele/imunologia , Células Th1/imunologia , Animais , Feminino , Interleucina-1/biossíntese , Interleucina-12/biossíntese , Subunidade p40 da Interleucina-12 , Interleucina-6/biossíntese , Larva/efeitos da radiação , Glicoproteínas de Membrana/fisiologia , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Receptores de Superfície Celular/fisiologia , Schistosoma mansoni/efeitos da radiação , Pele/citologia , Receptores Toll-Like , VacinaçãoRESUMO
Protective immunity against Schistosoma mansoni infection correlates with increased levels of IgE and blood eosinophilia which are considered as markers of anti-parasitic cell-mediated immunity. IL-5 participates as well in the induction and regulation of IgE and eosinophilia, consequently in the development of acquired immunity. Swiss Webster female mice were subcutaneously injected with either 50 microg of gamma-irradiated cercarial homogenate (400 Gy) twice weekly for three weeks alone or plus a single dose of IL-12 (0.8 ng/Kg). The efficiency of immunization regimens were assessed 45 days post infection with 100 live cercariae/mouse by the number of worm burden, ova count, production of IL-5, eosinophils, and IgE levels in the vaccinated groups compared with the non-immunized group. The results demonstrated a significant reduction of ova count in the livers of vaccinated groups (57.19 and 40.13%) and worm couples compared with the non -immunized group. Furthermore, a decrease of IL-5 level as well as eosinopenia was recorded in both vaccinated groups. Scanning electron microscope (SEM) of adult worms recovered from the immunized groups revealed marked damage on the tegumental surface in males rather than females as well as constrictions and intensive corrugation of intertubercles.