RESUMO
Background: Sepsis-induced acute lung injury (ALI) is a clinical syndrome characterized by excessive uncontrolled inflammation. Photobiomodulation such as light-emitting diode (LED) irradiation has been used to attenuate inflammatory disease. Objective: The protective effect of 630 nm LED irradiation on sepsis-induced ALI remains unknown. The purpose of this study was to investigate the role of 630 nm LED irradiation in sepsis-induced ALI and its underlying mechanism. Methods and results: C57BL/6 mice were performed cecal ligation and puncture (CLP) for 12 h to generate experimental sepsis models. Histopathology analysis showed that alveolar injury, inflammatory cells infiltration, and hemorrhage were suppressed in CLP mice after 630 nm LED irradiation. The ratio of wet/dry weigh of lung tissue was significantly inhibited by irradiation. The number of leukocytes was reduced in bronchoalveolar lavage fluid. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) results and enzyme-linked immunosorbent assay showed that 630 nm LED irradiation significantly inhibited the mRNA and protein levels of M1 macrophage-related genes in the lung of CLP-induced septic mice. Meanwhile, LED irradiation significantly inhibited signal transducer and activator of transcription 1 (STAT1) phosphorylation in the lung of septic mice. In vitro experiments showed that 630 nm LED irradiation significantly inhibited M1 genes mRNA and protein expression in THP-1-derived M1 macrophages without affecting the cell viability. LED irradiation also significantly inhibited the level of STAT1 phosphorylation in THP-1-derived M1 macrophages. Conclusions: We concluded that 630 nm LED is promising as a treatment against ALI through inhibiting M1 macrophage polarization, which is associated with the downregulation of STAT1 phosphorylation.
Assuntos
Lesão Pulmonar Aguda , Terapia com Luz de Baixa Intensidade , Sepse , Camundongos , Animais , Camundongos Endogâmicos C57BL , Lesão Pulmonar Aguda/complicações , Lesão Pulmonar Aguda/tratamento farmacológico , Macrófagos , Sepse/complicações , Sepse/radioterapia , Sepse/tratamento farmacológico , RNA MensageiroRESUMO
Although favorable immune responses to low-dose irradiation (LDI) have been observed in normal mice, i.e., a hormesis effect, little is known about the effects of LDI in infectious diseases. In this study, we examined the effects of LDI on mice with sepsis, a severe and often lethal hyperinflammatory response to bacteria. Female C57BL/6 mice were whole-body irradiated with 10cGy 48h before Escherichia coli infection, and survival, bacterial clearance, cytokines, and antioxidants were quantified. LDI pretreatment significantly increased survival from 46.7% in control mice to 75% in mice with sepsis. The bacterial burden was significantly lower in the blood, spleen, and kidney of LDI-treated mice than in those of control septic mice. The levels of pro-inflammatory cytokines, e.g., IL-1ß and IL-6, as well as anti-inflammatory IL-10 were markedly reduced in pre-LDI septic mice. Nitric oxide production by peritoneal macrophages was also reduced in pre-LDI septic mice. Immune cells in the spleen increased and Nrf2 and HO-1 were induced in pre-LDI septic mice. LDI stimulates the immune response and minimizes lethality in septic mice via enhanced bacterial clearance and reduced initial proinflammatory responses.
Assuntos
Infecções por Escherichia coli/radioterapia , Sepse/radioterapia , Irradiação Corporal Total , Animais , Contagem de Colônia Microbiana , Citocinas/sangue , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/sangue , Infecções por Escherichia coli/imunologia , Infecções por Escherichia coli/microbiologia , Feminino , Rim/microbiologia , Rim/efeitos da radiação , Macrófagos Peritoneais/metabolismo , Macrófagos Peritoneais/efeitos da radiação , Camundongos , Camundongos Endogâmicos C57BL , Óxido Nítrico/metabolismo , Células RAW 264.7 , Sepse/sangue , Sepse/imunologia , Sepse/microbiologia , Baço/microbiologia , Baço/efeitos da radiaçãoRESUMO
The results of investigation are adduced, concerning impact of ultraviolet irradiation and electric field of ultrahigh frequency in conjunction with local application of 1% ethanol solution of chlorophyllipt, using bandage, on prevention of purulent-septic complications occurrence with the soft tissues affection. There were operated on 107 patients. In 56 patients the proposed scheme of purulent-septic complications prophylaxis was applied. This permits to exclude the systemic application of antibiotics for prophylaxis after "clean" operations performance.
Assuntos
Assistência Ambulatorial/métodos , Sepse/prevenção & controle , Infecções dos Tecidos Moles/prevenção & controle , Infecção da Ferida Cirúrgica/prevenção & controle , Anti-Infecciosos Locais/administração & dosagem , Anti-Infecciosos Locais/uso terapêutico , Bandagens , Clorexidina/administração & dosagem , Clorexidina/uso terapêutico , Clorofila/administração & dosagem , Clorofila/uso terapêutico , Terapia Combinada , Drenagem , Combinação de Medicamentos , Terapia por Estimulação Elétrica/métodos , Humanos , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Sepse/tratamento farmacológico , Sepse/radioterapia , Infecções dos Tecidos Moles/tratamento farmacológico , Infecções dos Tecidos Moles/radioterapia , Supuração , Infecção da Ferida Cirúrgica/tratamento farmacológico , Infecção da Ferida Cirúrgica/radioterapia , Resultado do Tratamento , Terapia Ultravioleta/métodosRESUMO
Two comparable groups, 60 patients in each, were included in the study. Localization and area of the wounds, as well as basic treatment were equal in these groups. Patients of the main group received the additional photodynamic therapy with chlorine photosensibilizing agent. It led to the shortening of the wound clearing time and stimulation of the epithelisation together with fast decrease of bacterial load. Morphologic analysis showed faster inflammation decrease and granulation as well as lesser microcirculatory disorders in wounds treated with photodynamic therapy. Moreover, cicatrixes were more elastic and gentle in these patients.
Assuntos
Terapia com Luz de Baixa Intensidade/métodos , Sepse/radioterapia , Ferimentos e Lesões/complicações , Adolescente , Adulto , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Sepse/etiologia , Resultado do Tratamento , Cicatrização , Adulto JovemRESUMO
AIM: The aim of this study was to validate the computed tomography (CT) features of intra-abdominal hypertension (IAH) by relating them to the clinical measurement of intra-abdominal pressure (IAP) in critically ill surgical patients. MATERIALS AND METHODS: The intra-vesical pressure was measured to reflect IAP in 24 critically ill patients. CT examinations obtained within 24h of IAP measurement were reviewed and scored independently by two consultant radiologists. Each CT examination was scored for the seven proposed features of IAH. Images obtained during the presence of IAH were compared with those obtained in the absence of IAH. RESULTS: Forty-eight abdominal CT examinations were evaluated, of which 18 (38%) were obtained in the presence of IAH, whereas eight (17%) were obtained in the presence of abdominal compartment syndrome (ACS). At CT, the round belly sign (RBS) and bowel wall thickening with enhancement (BWTE) were significantly more frequently detected during the presence of IAH than when the IAP was less than 12 mmHg (78 versus 20% of examinations, p<0.001 and 39 versus 3% of examinations, p=0.003, respectively), but only BWTE was significantly associated with the presence of ACS (40 versus 11% of examinations, p=0.047). CONCLUSION: The presence of RBS and BWTE on CT images of critically ill surgical patients should alert clinicians to the possibility of presence of IAH and ACS, and prompt measurement of the IAP and consideration of suitable interventions.
Assuntos
Síndromes Compartimentais/diagnóstico por imagem , Pancreatite Crônica/diagnóstico por imagem , Radiografia Abdominal/métodos , Sepse/radioterapia , Tomografia Computadorizada por Raios X/normas , Doença Aguda , Adulto , Idoso , Síndromes Compartimentais/etiologia , Síndromes Compartimentais/fisiopatologia , Estado Terminal , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Pancreatite Crônica/etiologia , Pancreatite Crônica/fisiopatologia , Pressão , Estudos Prospectivos , Sensibilidade e Especificidade , Sepse/fisiopatologiaAssuntos
Hélio , Falência Renal Crônica/terapia , Transplante de Rim , Lasers , Neônio , Radioisótopos , Diálise Renal , Sepse/radioterapia , HumanosRESUMO
The complications and side-effects of laser therapy in 229 patients with septic-purulent diseases were analyzed. Metal taste in oral cavity, sleepiness, vertigo, weakness, sense of heat were the most frequently occurring side-effects of laser therapy observed.
Assuntos
Lasers/efeitos adversos , Cuidados Pós-Operatórios , Sepse/radioterapia , Supuração/radioterapia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Sangue/efeitos da radiação , Feminino , Humanos , Terapia a Laser , Masculino , Pessoa de Meia-IdadeRESUMO
The paper reviews the results of intravascular laser radiation (ILR) for pyoseptic complications in 8 patients with otogenic and 2 patients with rhinogenic meningitis, 1 patient with cavernous sinus thrombosis and 5 patients with Dupuytren's phlegmon. The adjuvant to conventional treatment use of ILR activates immune system, improves rheological blood characteristics, enhances tissue regeneration.
Assuntos
Terapia a Laser , Sepse/radioterapia , Adulto , Sangue/efeitos da radiação , Humanos , Masculino , Pessoa de Meia-Idade , Otolaringologia , Sepse/etiologia , Sepse/patologia , SupuraçãoAssuntos
Injúria Renal Aguda/complicações , Transfusão de Sangue Autóloga , Sangue/efeitos da radiação , Falência Renal Crônica/complicações , Flebite/radioterapia , Sepse/radioterapia , Terapia Ultravioleta , Infecção dos Ferimentos/radioterapia , Injúria Renal Aguda/sangue , Humanos , Falência Renal Crônica/sangue , Flebite/sangue , Flebite/etiologia , Dosagem Radioterapêutica , Sepse/sangue , Sepse/etiologia , Infecção dos Ferimentos/sangue , Infecção dos Ferimentos/etiologiaRESUMO
The experience with the use of ultraviolet irradiation (UVI) of the blood in 98 patients with purulent-inflammatory disease is presented. UVI of the blood has considerably improved the results of treatment of the patients. The highest effectiveness of UVI of the blood is noted in treatment of chromosepsis. The treatment of psoriasis by the mentioned method appeared ineffective.