RESUMO
Laboratory chemicals and reagents normally contain trace amounts of iron salts sufficient to catalyse free radical reactions. This iron contamination can be removed from buffers and reagents using a dialysis sac containing a high-affinity iron-binding protein like conalbumin or transferrin without altering the pH value of the fluid.
Assuntos
Soluções Tampão/isolamento & purificação , Ferro/isolamento & purificação , Bleomicina , Conalbumina , Diálise , Indicadores e Reagentes/isolamento & purificação , TransferrinaRESUMO
Treatment of Quadrol buffer with isothiocyanatophenylthiocarbamylaminoethylaminopropyl glass (DITC glass) substantially reduces the impurities observed when this buffer is used with the spinning cup sequenator. Use of DITC glass-treated Quadrol buffer permits identification of PTH-amino acids from protein degradations down to 100 pmol.
Assuntos
Sequência de Aminoácidos , Soluções Tampão/isolamento & purificação , Etilenodiaminas/isolamento & purificação , Autoanálise/instrumentação , Cromatografia/métodos , MicroquímicaRESUMO
A simple procedure for obtaining useful narrow-pH-range ampholytes from inexpensive laboratory-synthesized ampholytes by preparative isoelectric focusing in Pevikon is described. The narrow-range ampholytes prepared in this way are comparable to commercial ampholyte preparations as judged by conductivity, buffer capacity, pH gradient formation, and resolving power. These inexpensive narrow-range ampholytes are particularly well suited to preparative isoelectric focusing applications requiring large quantities of ampholytes.