Inactivation of SARS-CoV-2 in Serum Using Physical Methods.

Since 2019, many studies on coronavirus disease 2019, which has caused extensive damage as a pandemic, have been ongoing on a global scale. These include serological and biochemical studies using sera from patients and animal models. Testing with these sera must be performed after inactivation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Heat treatment, UV irradiation, and/or gamma-ray irradiation have been used to inactivate viruses in the serum. Determining the inactivation conditions that ensure the inactivation of viruses and minimize the effect on test results after inactivation is important to ensure worker safety and the accuracy of test results. In this study, serum samples containing SARS-CoV-2 were subjected to heat, UV irradiation, and gamma irradiation to determine optimal inactivation conditions. The viral titers were below the detection limit after heating at 56°C for 1 h or 60°C for 15 min, UV-B irradiation with a transilluminator for 30 min, or gamma-ray irradiation with 60 Co at 10 kGy. These results provide useful information for safe serological and biochemical experiments.

South-South Cooperation: Brazilian experiences in South America and Africa / Cooperação Sul-Sul: experiências brasileiras na América do Sul e na África

Over recent years Brazil has played an increasingly active role internationally, the result of its model of integration and its foreign policy directives. The health sector is a valuable and strategic area for Brazilian technical cooperation to achieve various objectives, including its development goals. This article describes the main directives of Brazilian foreign policy, conceptually defining and characterizing South-South Cooperation, illustrated through an analysis of two Brazilian technical cooperation initiatives in healthcare: one in South America, the other in Africa. The study concludes that, irrespective of the interests and power asymmetries existing in South-South Cooperation, the objectives of this cooperation were achieved through the technical work.

Nos últimos anos, o Brasil foi ativo no âmbito internacional, tanto por seu modelo de inserção como pelas diretrizes de política externa. O setor saúde é uma ferramenta valiosa e estratégica utilizada pela cooperação técnica brasileira para lograr seus objetivos de desenvolvimento. Este artigo descreve as principais diretrizes de política externa brasileira, conceitua e caracteriza a Cooperação Sul-Sul, ilustrada mediante análise de duas iniciativas de cooperação técnica em saúde do Brasil: na América do Sul e na África. O estudo conclui que, independentemente dos interesses e das assimetrias de poder que existem na Cooperação Sul-Sul, os objetivos dessa cooperação foram alcançados por meio do trabalho técnico.

Hepatitis C virus quantification in serum and saliva of HCV-infected patients

The hepatitis C virus (HCV) can be detected in blood and other bodily fluids, such as saliva, semen and gastric juices. The aim of this study was to compare the HCV viral loads in the serum and saliva of infected patients. Twenty-nine patients with detectable HCV RNA in their serum and saliva were included in this study. The HCV viral loads were determined through quantitative real-time polymerase chain reactions. The median viral RNA levels were 5.78 log10 copies in the serum and 3.32 log10 copies in the saliva. We observed that the salivary HCV viral load was significantly lower than the viral load in the serum. Further studies are required to understand the role of saliva in the diagnosis, management and potential transmission of HCV.

Incidencia del virus herpes simplex en pacientes con infecciones agudas del sistema nervioso central / Incidence of herpes simplex virus infection in patients with acute central nervous system infections

Los Virus Herpes Simple son responsables de una variedad de infecciones a nivel de mucosas bucal y genital, son neurotrópicos, capaces de alojarse en células nerviosas y permanecer latentes con subsecuentes reactivaciones, en algunos casos pueden provocar meningitis y encefalitis. tienen una morbilidad significativa y una mortalidad elevada. El presente estudio tiene como objetivo determinar la incidencia de VHS en la etiología de los síndromes agudos del SNC mediante la detección en suero y LCR de anticuerpos específicos. Se estudiaron 93 muestras pareadas de pacientes con afecciones neurológicas que mostraron resultados negativos en el estudio bacteriológico de LCR y cuya relación Alb-LCR/Alb-suero fue <0,0075. Los anticuerpos fueron detectados por el método de inmunoensayo enzimático Elisa: Enzygnost® anti virus VHS/IgM-IgG (Behring, 1994), donde 1,2% (1/93) de las muestras de suero resultaron positivas para IgM; 89,24% (83/93)de los sueros resultaron positivos para IgG; y 27,39% (20/93) de las muestras de LCR presentaron anti-IgG positivo. La presencia de IgG en LCR sugiere la producción intratecal de estos anticuerpos en el SNC, lo que muestra una participación importante de este agente viral en las infecciones y enfermedades asociadas al SNC

Herpes simplex viruses are agents responsible for a variety of infections on buccal and genital mucosa; they are neurotropic, capable of lodging in the nerve cells and remaining latent with subsequent reactivations; in some cases, they can provoke meningitis and encephalitis; and they have a significant morbidity and are associated with a high mortality. The present project aims to determine HSV’s effect on the etiology of acute central nervous system (CNS) syndromes using the detection of specific antibodies in serum and cerebrospinal fluid. The 93 paired samples from patients with neurological affectations studied demonstrated negative results in the LCR bacteriological study and their Alb-LCR/Alb-serum relation was < 0.0075. Antibodies were detected by the enzymatic immunoassay ELISA method, where 1.2% (1/93) of the serum samples turned out positive for IgM; 89.24% (83/93) of the serum samples resulted positive for IgG; and 27.39% (20/93) of LCR samples were positive for anti-IgG. IgG presence in LCR suggested the intrathecal production of these antibodies inside the CNS, which demonstrates a significant participation by this viral agent in infections and diseases associated with the CNS

Diagnóstico de laboratorio de la infección por el virus de la hepatitis C / Laboratory diagnosis of hepatitis C virus infection

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