RESUMO
The aerobic respiratory chain of Pyrobaculum oguniense is expressed constitutively even under anaerobic conditions. The membranes of both aerobically and anaerobically grown cells show oxygen consumption activity with NADH as substrate, bovine cytochrome c oxidase activity and TMPD oxidase activity. Spectroscopic analysis and haem analysis of membranes of aerobically grown cells show the presence of cytochrome b(559), cytochrome c(551) and haem Op1 containing cytochrome c oxidase in aerobically and anaerobically grown cells, and haem As containing cytochrome c oxidase in aerobically grown cells. The gene clusters of SoxB-type and SoxM-type haem copper oxidase and cytochrome bc complex have been cloned and sequenced and the regulation of these genes was analysed. The Northern blot analysis indicated that the constitutive transcription of the gene cluster of SoxB-type haem-copper oxidase and cytochrome bc complex is observed under both aerobic and anaerobic conditions, and the transcription of the operon of SoxM-type haem-copper oxidase was stimulated under aerobic conditions. Furthermore, the presence of the binding residues for CuA in subunit II of both SoxB- and SoxM-type haem-copper oxidase suggests that these haem-copper oxidases are cytochrome c oxidases.
Assuntos
Regulação da Expressão Gênica em Archaea , Consumo de Oxigênio , Thermoproteaceae/crescimento & desenvolvimento , Thermoproteaceae/fisiologia , Aerobiose , Anaerobiose , Animais , Proteínas Arqueais/química , Proteínas Arqueais/genética , Proteínas Arqueais/metabolismo , Bovinos , Grupo dos Citocromos c/genética , Grupo dos Citocromos c/metabolismo , Complexo IV da Cadeia de Transporte de Elétrons/genética , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Heme/metabolismo , Temperatura Alta , Dados de Sequência Molecular , NAD/metabolismo , Oxirredutases/genética , Oxirredutases/metabolismo , Oxirredutases N-Desmetilantes/genética , Oxirredutases N-Desmetilantes/metabolismo , Análise de Sequência de DNA , Thermoproteaceae/genéticaAssuntos
Betaproteobacteria/genética , Clostridium perfringens/genética , Genoma Arqueal , Genoma Bacteriano , Thermoproteaceae/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/fisiologia , Betaproteobacteria/patogenicidade , Clostridium perfringens/patogenicidade , Gangrena Gasosa/microbiologia , Genes Bacterianos , Doenças das Plantas/microbiologia , Análise de Sequência de DNA , Thermoproteaceae/fisiologia , Virulência/genéticaRESUMO
Two novel hyperthermophilic, rod-shaped crenarchaeotes were isolated from an acidic hot spring in the Philippines. Cells were mostly straight or slightly curved rods 0.4-0.7 micron in width. Bent cells, branched cells, and cells bearing globular bodies were commonly observed. The isolates were heterotrophs and grew anaerobically and microaerobically. The addition of archaeal cell extract or a vitamin mixture to the medium significantly stimulated growth. The isolates grew over a temperature range of 60-92 degrees C, and optimally around 85 degrees C and grew over a pH range of 2.3-6.4, and optimally at pH 3.7-4.2. The isolates utilized glycogen, gelatin, beef extract, peptone, tryptone and yeast extract as carbon sources. They required sulfur, thiosulfate or sulfate as electron acceptors. The lipids mainly consisted of various cyclized glycerol-bisdiphytanyl-glycerol tetraethers. The G+C content of the genomic DNAs was 43 mol%. The 16S rDNA contained two small introns. The comparison of the 16S rDNA exon sequences revealed that they represented an independent lineage in the family Thermoproteaceae. The two strains were included in a single species because of high levels of DNA-DNA relatedness. From these results, Caldivirga maquilingensis gen. nov., sp. nov. is proposed in the family Thermoproteaceae to accommodate these isolates. The type strain of C. maquilingensis is strain IC-167T (= JCM 10307T = MCC-UPLB 1200T = ANMR 0178T).
Assuntos
Água Doce/microbiologia , Thermoproteaceae/classificação , DNA Arqueal/química , DNA Arqueal/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Éxons , Genes de RNAr , Íntrons , Dados de Sequência Molecular , Fenótipo , Filipinas , Filogenia , RNA Ribossômico 16S/genética , Thermoproteaceae/isolamento & purificação , Thermoproteaceae/fisiologia , Thermoproteaceae/ultraestrutura , Microbiologia da ÁguaRESUMO
Three strains of novel, extremely thermophilic, rod-shaped crenarchaeotes were isolated from acidic hot spring areas in Japan. Cells of the three stains were straight or slightly curved rods and occasionally branched out singly or extensively, or had spherical bodies protruding at the ends of the cells. They were heterotrophs that grew anaerobically or microaerobically. The presence of CO2 in the gas phase, archaeal cell-extracts and a vitamin mixture stimulated growth of the strains. Growth occurred at 45-82 degrees C and pH 2.6-5.9 and was optimal around 75 degrees C and pH 4.0. The strains utilized glycogen, starch, gelatin and various proteinaceous complex compounds as carbon sources. They required sulfur, thiosulfate or L-cystine as possible electron acceptors. The lipids mainly consisted of various cyclic glycerol-bisdiphytanyl-glycerol tetraethers. The G+C contents of the genomic DNAs were 52 mol%. Comparison of the 16S rDNA sequences indicated that they belonged to a separate lineage in the family Thermoproteaceae. The three strains were included in a single species due to high levels of DNA-DNA hybridization values. Based upon these results, the new isolates were assigned to a new genus and species in the family Thermoproteaceae. Thermocladium modestius gen. nov., sp. nov. The type strain is Thermocladium modestius IC-125T (= JCM 10088T).