RESUMO
Proper cell division at the mid-site of Gram-negative bacteria reflects stringent regulation by the min system (MinC, MinD and MinE). Herein we report crystal structure of the C-terminal domain of MinC from Escherichia coli (EcMinCCTD). The MinCCTD beta helical domain is engaged in a tight homodimer, similar to Thermotoga maritima MinCCTD (TmMinCCTD). However, both EcMinCCTD and TmMinCCTD lack an α-helix (helix3) at their C-terminal tail, in comparison to Aquifex aerolicu MinCCTD (AaMinCCTD) which forms an extra interaction interface with MinD. To understand the role of this extra binding element in MinC/MinD interactions, we fused this helix (Aahelix3) to the C-terminus of EcMinC and examined its effect on cell morphology and cell growth. Our results revealed that Aahelix3 impaired normal cell division in vivo. Furthermore, results of a co-pelleting assay and binding free energy calculation suggested that Aahelix3 plays an essential role in AaMinCD complex formation, under the circumstance of lacking MinE in A. aerolicu. Combining these results with sequence analysis of MinC and MinD in different organisms, we propose an evolutionary relationship to rationalize different mechanisms in cell division positioning in various organisms.
Assuntos
Adenosina Trifosfatases/genética , Proteínas de Ciclo Celular/genética , Divisão Celular , Proteínas de Escherichia coli/genética , Escherichia coli/genética , Proteínas de Membrana/genética , Adenosina Trifosfatases/química , Adenosina Trifosfatases/metabolismo , Sequência de Aminoácidos , Sítios de Ligação , Proteínas de Ciclo Celular/química , Proteínas de Ciclo Celular/metabolismo , Clonagem Molecular , Cristalografia por Raios X , Escherichia coli/citologia , Escherichia coli/metabolismo , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/metabolismo , Evolução Molecular , Expressão Gênica , Vetores Genéticos/química , Vetores Genéticos/metabolismo , Proteínas de Membrana/química , Proteínas de Membrana/metabolismo , Modelos Moleculares , Ligação Proteica , Conformação Proteica em alfa-Hélice , Conformação Proteica em Folha beta , Domínios e Motivos de Interação entre Proteínas , Multimerização Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Termodinâmica , Thermotoga maritima/citologia , Thermotoga maritima/genética , Thermotoga maritima/metabolismoRESUMO
Thermotoga maritima cells are distinguished by a sheath-like structure called the toga that loosely encloses single or multiple cells. During growth, and particularly at late phases of population growth, the toga distends from the poles of many cells. Little is known about this phenomenon so this study presents basic information about this process. We first provide quantitative data demonstrating that cells showing toga distensions increase in number during growth and that the phenomenon is not due to acidification of their growth medium. Comparisons of the area enclosed by these distended togas to the area of the cytoplasm show that the toga continues to grow as the growth of the cytoplasm ceases. Measuring the expression of many genes involved in toga composition and biosynthesis showed a 5.2-, 7.9- and 3-fold increase in the expression of toga structural protein genes ompB (porin), ompA1 and ompA2 (alpha helical, transperiplasm anchors), respectively. Additionally, expression of the putative pyruvyl transferase gene (csaB) was upregulated 4.4-fold in stationary phase, while the beta barrel assembly factor gene (bamA) showed only a 1.2-fold increase in expression. These findings demonstrate that toga distension is an active process and one that needs further investigation so we can understand the selective forces that operate in high-temperature environments.
Assuntos
Membrana Celular/fisiologia , Thermotoga maritima/citologia , Thermotoga maritima/crescimento & desenvolvimento , Proteínas da Membrana Bacteriana Externa/genética , Proteínas de Bactérias/genética , Membrana Celular/genética , Parede Celular/genética , Parede Celular/fisiologia , Meios de Cultura/química , Regulação Bacteriana da Expressão Gênica , Temperatura Alta , Porinas/genéticaRESUMO
Engineered biological systems are used extensively for the production of high value and commodity organics. On the other hand, most inorganic nanomaterials are still synthesized via chemical routes. By engineering cellular compartments, functional nanoarchitectures can be produced under environmentally sustainable conditions. Encapsulins are a new class of microbial nanocompartments with promising applications in nanobiotechnology. Here, we engineer the Thermotoga maritima encapsulin EncTm to yield a designed compartment for the size-constrained synthesis of silver nanoparticles (Ag NPs). These Ag NPs exhibit uniform shape and size distributions as well as long-term stability. Ambient aqueous conditions can be used for Ag NP synthesis, while no reducing agents or solvents need to be added. The antimicrobial activity of the synthesized protein-coated or shell-free Ag NPs is superior to that of silver nitrate and citrate-capped Ag NPs. This study establishes encapsulins as an engineerable platform for the synthesis of biogenic functional nanomaterials.
Assuntos
Anti-Infecciosos , Nanopartículas Metálicas , Nanotecnologia/métodos , Prata , Thermotoga maritima/citologia , Thermotoga maritima/metabolismo , Anti-Infecciosos/química , Anti-Infecciosos/metabolismo , Anti-Infecciosos/farmacologia , Compartimento Celular , Estabilidade de Medicamentos , Engenharia Genética/métodos , Nanopartículas Metálicas/química , Testes de Sensibilidade Microbiana , Prata/química , Prata/farmacologia , Espectrometria por Raios XRESUMO
Nature uses bottom-up approaches for the controlled assembly of highly ordered hierarchical structures with defined functionality, such as organelles, molecular motors, and transmembrane pumps. The field of bionanotechnology draws inspiration from nature by utilizing biomolecular building blocks such as DNA, proteins, and lipids, for the (self-) assembly of new structures for applications in biomedicine, optics, or electronics. Among the toolbox of available building blocks, proteins that form cage-like structures, such as viruses and virus-like particles, have been of particular interest since they form highly symmetrical assemblies and can be readily modified genetically or chemically both on the outer or inner surface. Bacterial encapsulins are a class of nonviral protein cages that self-assemble in vivo into stable icosahedral structures. Using teal fluorescent proteins (TFP) engineered with a specific native C-terminal docking sequence, we report the molecular self-sorting and selective packaging of TFP cargo into bacterial encapsulins during in vivo assembly. Using native mass spectrometry techniques, we show that loading of either monomeric or dimeric TFP cargo occurs with unprecedented high fidelity and exceptional loading accuracy. Such self-assembling systems equipped with self-sorting capabilities would open up exciting opportunities in nanotechnology, for example, as artificial (molecular storage or detoxification) organelles or as artificial cell factories for in situ biocatalysis.
Assuntos
Brevibacterium/citologia , Corantes Fluorescentes/metabolismo , Proteínas de Fluorescência Verde/metabolismo , Thermotoga maritima/citologia , Brevibacterium/metabolismo , Corantes Fluorescentes/análise , Proteínas de Fluorescência Verde/análise , Modelos Moleculares , Multimerização Proteica , Transporte Proteico , Thermotoga maritima/metabolismoRESUMO
This study was conducted to assess the gene duplication and diversification of tandem cellulase genes in thermophilic bacteria. The tandem cellulase genes cel5C and cel5D were cloned from Thermotoga maritima MSB8, and a survey of the thermophilic bacterial genome for tandem cel genes from the databases was carried out. A clone having 2.3 kb fragment from T. maritima MSB8 showed cellulase activity, which had two open reading frames in tandem (cel5C and cel5D). The cel5C gene has 954 bp, which encodes a protein of 317 amino acid residues with a signal peptide of 23 amino acids, and the other gene cel5D consisting of 990 bp encoding a protein of 329 amino acid residues. These two proteins have similarity with the enzymes of glycosyl hydrolase family 5. From the enzyme assay, it was observed that Cel5C was extracellular and Cel5D was intracellular cellulase. Phylogenetic and homology matrix analyses of DNA and protein sequences revealed that family 12 cellulase enzymes Cel12A and Cel12B displayed higher homology (>50 %), but Cel5C and Cel5D enzymes belong to family 5 displayed lower homology (<30 %). In addition, repeated and mirror sequences in tandem genes are supposed to show the existence of gene duplication and recombination.
Assuntos
Celulases/genética , Duplicação Gênica , Recombinação Genética , Thermotoga maritima/enzimologia , Thermotoga maritima/genética , Celulases/química , Clonagem Molecular , DNA Bacteriano/genética , Evolução Molecular , Espaço Extracelular/enzimologia , Glicosídeo Hidrolases/genética , Espaço Intracelular/enzimologia , Modelos Moleculares , Filogenia , Conformação Proteica , Thermotoga maritima/citologiaRESUMO
The unifying structural characteristic of members of the bacterial order Thermotogales is their toga, an unusual cell envelope that includes a loose-fitting sheath around each cell. Only two toga-associated structural proteins have been purified and characterized in Thermotoga maritima: the anchor protein OmpA1 (or Ompα) and the porin OmpB (or Ompß). The gene encoding OmpA1 (ompA1) was cloned and sequenced and later assigned to TM0477 in the genome sequence, but because no peptide sequence was available for OmpB, its gene (ompB) was not annotated. We identified six porin candidates in the genome sequence of T. maritima. Of these candidates, only one, encoded by TM0476, has all the characteristics reported for OmpB and characteristics expected of a porin including predominant ß-sheet structure, a carboxy terminus porin anchoring motif, and a porin-specific amino acid composition. We highly enriched a toga fraction of cells for OmpB by sucrose gradient centrifugation and hydroxyapatite chromatography and analyzed it by LC/MS/MS. We found that the only porin candidate that it contained was the TM0476 product. This cell fraction also had ß-sheet character as determined by circular dichroism, consistent with its enrichment for OmpB. We conclude that TM0476 encodes OmpB. A phylogenetic analysis of OmpB found orthologs encoded in syntenic locations in the genomes of all but two Thermotogales species. Those without orthologs have putative isofunctional genes in their place. Phylogenetic analyses of OmpA1 revealed that each species of the Thermotogales has one or two OmpA homologs. T. maritima has two OmpA homologs, encoded by ompA1 (TM0477) and ompA2 (TM1729), both of which were found in the toga protein-enriched cell extracts. These annotations of the genes encoding toga structural proteins will guide future examinations of the structure and function of this unusual lineage-defining cell sheath.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Membrana Celular/genética , Evolução Molecular , Genes Bacterianos/genética , Proteômica/métodos , Thermotoga maritima/genética , Sequência de Aminoácidos , Proteínas da Membrana Bacteriana Externa/isolamento & purificação , Sequência de Bases , Centrifugação com Gradiente de Concentração , Cromatografia , Dicroísmo Circular , Durapatita , Funções Verossimilhança , Dados de Sequência Molecular , Filogenia , Porinas/química , Porinas/genética , Multimerização Proteica , Homologia de Sequência do Ácido Nucleico , Especificidade da Espécie , Sintenia/genética , Thermotoga maritima/citologiaRESUMO
An efficient and economical medium--Thermotoga maritima basal medium (TMB)--was designed for the cultivation of T. maritima under either liquid or solid conditions. When the broth was flushed with N2 or CO2 throughout cell growth in a 10-L fermentor (pH controlled to 6.5), the maximum cell density (OD600) on TMB containing 1% glucose rose to 2.0 or higher (1.63 x 10(9) cells mL(-1)). Sheath-less cells observed by electron microscopy were captured during growth in the fermentor. Using a two-layer plating method, isolated single-well colonies were consistently obtained within 24 h on the TMB in modified tissue culture flasks. The minimal inhibitory chloramphenicol concentrations for T. maritima on TMB agar were 5 microg mL(-1) after 24 h and 48 h, and 25 microg mL(-1) at 72 h.
Assuntos
Técnicas Bacteriológicas/métodos , Meios de Cultura , Thermotoga maritima/crescimento & desenvolvimento , Anaerobiose , Antibacterianos/farmacologia , Metabolismo dos Carboidratos , Contagem de Colônia Microbiana , Meios de Cultura/química , Fermentação , Temperatura Alta , Testes de Sensibilidade Microbiana , Microscopia Eletrônica , Thermotoga maritima/citologia , Thermotoga maritima/efeitos dos fármacos , Thermotoga maritima/metabolismoRESUMO
In bacterial chemotaxis, an assembly of transmembrane receptors, the CheA histidine kinase and the adaptor protein CheW processes environmental stimuli to regulate motility. The structure of a Thermotoga maritima receptor cytoplasmic domain defines CheA interaction regions and metal ion-coordinating charge centers that undergo chemical modification to tune receptor response. Dimeric CheA-CheW, defined by crystallography and pulsed ESR, positions two CheWs to form a cleft that is lined with residues important for receptor interactions and sized to clamp one receptor dimer. CheW residues involved in kinase activation map to interfaces that orient the CheW clamps. CheA regulatory domains associate in crystals through conserved hydrophobic surfaces. Such CheA self-contacts align the CheW receptor clamps for binding receptor tips. Linking layers of ternary complexes with close-packed receptors generates a lattice with reasonable component ratios, cooperative interactions among receptors and accessible sites for modification enzymes.
Assuntos
Proteínas de Bactérias/metabolismo , Quimiotaxia , Proteínas de Membrana/metabolismo , Proteínas Quinases/química , Proteínas Quinases/metabolismo , Thermotoga maritima/enzimologia , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Cristalografia por Raios X , Citoplasma/química , Citoplasma/genética , Citoplasma/metabolismo , Dimerização , Espectroscopia de Ressonância de Spin Eletrônica , Proteínas de Membrana/química , Proteínas de Membrana/genética , Proteínas Quimiotáticas Aceptoras de Metil , Modelos Moleculares , Ligação Proteica , Dobramento de Proteína , Proteínas Quinases/genética , Estrutura Quaternária de Proteína , Transdução de Sinais , Thermotoga maritima/citologia , Thermotoga maritima/genéticaRESUMO
The gene for a novel alpha-amylase, designated AmyC, from the hyperthermophilic bacterium Thermotoga maritima was cloned and heterologously overexpressed in Escherichia coli. The putative intracellular enzyme had no amino acid sequence similarity to glycoside hydrolase family (GHF) 13 alpha-amylases, yet the range of substrate hydrolysis and the product profile clearly define the protein as an alpha-amylase. Based on sequence similarity AmyC belongs to a subgroup within GHF 57. On the basis of amino acid sequence similarity, Glu185 and Asp349 could be identified as the catalytic residues of AmyC. Using a 60-min assay, the maximum hydrolytic activity of the purified enzyme, which was dithiothreitol dependent, was found to be at 90 degrees C. AmyC displayed a remarkably high pH optimum of pH 8.5 and an unusual sensitivity towards both ATP and EDTA.