RESUMO
A new secondary metabolite, named altertoxin IV (1), together with altertoxin II (2), was isolated from the fermentation broth of Alternaria tenuissima, an endophytic fungal strain residing in the stem of Tribulus terrestris L. The structure of new compound 1 was established by HR-ESI-MS, multinuclear NMR spectroscopy, and single crystal X-ray diffraction method. In their in vitro bioassay, compound 2 exhibited moderate cytotoxic activity against PC-3 cell lines with an IC50 value of 14.28 µM.
Assuntos
Alternaria/química , Benzo(a)Antracenos/isolamento & purificação , Micotoxinas/isolamento & purificação , Algoritmos , Benzo(a)Antracenos/química , Benzo(a)Antracenos/farmacologia , China , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Micotoxinas/química , Micotoxinas/farmacologia , Ressonância Magnética Nuclear Biomolecular , Caules de Planta/microbiologia , Tribulus/microbiologia , Difração de Raios XRESUMO
Scanning electron microscopic observations were made on the micro-organisms of root nodules of Tribulus terrestris L. The results showed that nodules of T. terrestris contained dual infection consisting of Rhizobium sp. and Newmania karachiensis. Based on these observations, T. terrestris should be grouped with nonlegume Parasponia-type bacterial nodules.