RESUMO
Colorado tick fever virus is transmitted by Dermacentor andersoni ticks. In Canada, these ticks are found in the southern regions of British Columbia (Rocky Mountains) and Alberta, as well as southwestern Saskatchewan. Colorado tick fever should be clinically suspected in patients presenting with a biphasic febrile illness and leukopenia following tick exposure in the appropriate geographic area.
Assuntos
Vetores Aracnídeos/virologia , Febre do Carrapato do Colorado/diagnóstico , Vírus da Febre do Carrapato do Colorado/genética , Dermacentor/virologia , Picadas de Carrapatos/diagnóstico , Idoso , Animais , Febre do Carrapato do Colorado/tratamento farmacológico , Febre do Carrapato do Colorado/fisiopatologia , Febre do Carrapato do Colorado/virologia , Vírus da Febre do Carrapato do Colorado/classificação , Vírus da Febre do Carrapato do Colorado/isolamento & purificação , Doxiciclina/uso terapêutico , Febre/fisiopatologia , Humanos , Leucopenia/fisiopatologia , Masculino , Saskatchewan , Picadas de Carrapatos/tratamento farmacológico , Picadas de Carrapatos/fisiopatologia , Picadas de Carrapatos/virologiaAssuntos
Vetores Aracnídeos/virologia , Coltivirus/isolamento & purificação , Infecções por Reoviridae , Carrapatos/virologia , Animais , Vírus da Febre do Carrapato do Colorado/classificação , Coltivirus/classificação , Coltivirus/ultraestrutura , Reservatórios de Doenças , Alemanha/epidemiologia , Humanos , Infecções por Reoviridae/diagnóstico , Infecções por Reoviridae/tratamento farmacológico , Infecções por Reoviridae/epidemiologia , Infecções por Reoviridae/transmissãoRESUMO
We report a genomic and morphologic study of the European Eyach (EYA) virus (genus Coltivirus, family Reoviridae) and a comparative analysis with the American Colorado tick fever (CTF) virus (the type species of the genus). The previously established, but distant, antigenic relationship between these viruses was strengthened by genetic findings (presence of cognate genes, amino acid identity between 55 and 88%, similar conserved terminal motifs, suspected read-through phenomenon in segment 9 of both viruses) and by indistinguishable ultramicroscopic morphologies. Moreover, putative constitutive modifying enzyme activities were suspected to be carried out by homologous viral proteins (RNA-dependent RNA polymerase, methyl/guanylyl transferase, NTPase). These findings, together with the comparative analysis to genomes of southeast Asian isolates, support the recent classification of arboviruses with 12 segments of dsRNA within two distinct genera (genus Coltivirus and genus Seadornavirus) and raise interesting questions about the evolutionary origins of coltiviruses. The previously proposed hypothesis that EYA virus was derived from an ancestral virus introduced in Europe with the migration of lagomorphs from North-America, would imply a divergence date between American and European isolates of over 50 million years ago (MYA). This analysis allows for the first time to propose an evolutionary rate for virus dsRNA genomes which was found to be in the order of 10(-8) to 10(-9) mutations/nt/year, a rate similar to that of dsDNA genomes.
