RESUMO
Several microbial genomes lack textbook-defined essential genes. If an essential gene is absent from a genome, then an evolutionarily independent gene of unknown function complements its function. Here, we identified frequent nonhomologous replacement of an essential component of DNA replication initiation, a replicative helicase loader gene, in Vibrionaceae. Our analysis of Vibrionaceae genomes revealed two genes with unknown function, named vdhL1 and vdhL2, that were substantially enriched in genomes without the known helicase-loader genes. These genes showed no sequence similarities to genes with known function but encoded proteins structurally similar with a viral helicase loader. Analyses of genomic syntenies and coevolution with helicase genes suggested that vdhL1/2 encodes a helicase loader. The in vitro assay showed that Vibrio harveyi VdhL1 and Vibrio ezurae VdhL2 promote the helicase activity of DnaB. Furthermore, molecular phylogenetics suggested that vdhL1/2 were derived from phages and replaced an intrinsic helicase loader gene of Vibrionaceae over 20 times. This high replacement frequency implies the host's advantage in acquiring a viral helicase loader gene.
Assuntos
DNA Helicases , Replicação do DNA , Filogenia , Vibrionaceae , Vibrionaceae/genética , Vibrionaceae/enzimologia , DNA Helicases/metabolismo , DNA Helicases/genética , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/genética , Proteínas Virais/genética , Proteínas Virais/metabolismo , Bacteriófagos/genética , Bacteriófagos/enzimologia , Evolução Molecular , Genoma Bacteriano , DnaB Helicases/metabolismo , DnaB Helicases/genética , Vibrio/genética , Vibrio/enzimologiaRESUMO
Collagenase from the gram-negative bacterium Grimontia hollisae strain 1706B (Ghcol) degrades collagen more efficiently even than clostridial collagenase, the most widely used industrial collagenase. However, the structural determinants facilitating this efficiency are unclear. Here, we report the crystal structures of ligand-free and Gly-Pro-hydroxyproline (Hyp)-complexed Ghcol at 2.2 and 2.4 Å resolution, respectively. These structures revealed that the activator and peptidase domains in Ghcol form a saddle-shaped structure with one zinc ion and four calcium ions. In addition, the activator domain comprises two homologous subdomains, whereas zinc-bound water was observed in the ligand-free Ghcol. In the ligand-complexed Ghcol, we found two Gly-Pro-Hyp molecules, each bind at the active site and at two surfaces on the duplicate subdomains of the activator domain facing the active site, and the nucleophilic water is replaced by the carboxyl oxygen of Hyp at the P1 position. Furthermore, all Gly-Pro-Hyp molecules bound to Ghcol have almost the same conformation as Pro-Pro-Gly motif in model collagen (Pro-Pro-Gly)10, suggesting these three sites contribute to the unwinding of the collagen triple helix. A comparison of activities revealed that Ghcol exhibits broader substrate specificity than clostridial collagenase at the P2 and P2' positions, which may be attributed to the larger space available for substrate binding at the S2 and S2' sites in Ghcol. Analysis of variants of three active-site Tyr residues revealed that mutation of Tyr564 affected catalysis, whereas mutation of Tyr476 or Tyr555 affected substrate recognition. These results provide insights into the substrate specificity and mechanism of G. hollisae collagenase.
Assuntos
Proteínas de Bactérias , Colágeno , Colagenases , Vibrionaceae , Proteínas de Bactérias/química , Colágeno/química , Colagenases/química , Hidroxiprolina/química , Especificidade por Substrato , Vibrionaceae/enzimologia , Água/química , Zinco/químicaRESUMO
A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84 g/L were obtained using glucose as the carbon source after 24 h of cultivation in shake flasks. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was synthesized when propionate was provided as secondary carbon source. Salinivibrio sp. TGB10 exhibited favorable tolerance to propionate. The use of 8 g/L propionate and 20 g/L glucose as combinational substrates yielded 1.45 g/L PHBV with a 3-hydroxyvalerate monomer content of 72.02 mol% in flask cultures. In bioreactor study, CDW of 33.45 g/L and PHBV titer of 27.36 g/L were obtained after 108 h of fed-batch cultivation. The results indicated that Salinivibrio sp. TGB10 is a promising halophilic bacterium for the production of PHBV with various polymer compositions.
Assuntos
Hidroxibutiratos/metabolismo , Poliésteres/metabolismo , Poli-Hidroxialcanoatos/metabolismo , Vibrionaceae/enzimologia , Reatores Biológicos , Ácidos Graxos Voláteis/metabolismo , Fermentação , Especificidade por Substrato , Açúcares/metabolismo , Vibrionaceae/crescimento & desenvolvimento , Microbiologia da ÁguaRESUMO
Salinivibrio proteolyticus M318, a halophilic bacterium isolated from fermented shrimp paste, is able to produce polyhydroxyalkanoate (PHA) from different carbon sources. In this study, we report the whole-genome sequence of strain M138, which comprises 2 separated chromosomes and 2 plasmids, and the complete genome contains 3,605,935 bp with an average GC content of 49.9%. The genome of strain M318 contains 3341 genes, 98 tRNA genes, and 28 rRNA genes. The 16S rRNA gene sequence and average nucleotide identity analysis associated with morphological and biochemical tests showed that this strain has high homology to the reference strain Salinivibrio proteolyticus DSM 8285. The genes encoding key enzymes for PHA and ectoine synthesis were identified from the bacterial genome. In addition, the TeaABC transporter responsible for ectoine uptake from the environment and the operon doeABXCD responsible for the degradation of ectoine were also detected. Strain M318 was able to produce poly(3-hydroxybutyrate) [P(3HB)] from different carbon sources such as glycerol, maltose, glucose, fructose, and starch. The ability to produce ectoines at different NaCl concentrations was investigated. High ectoine content of 26.2% of cell dry weight was obtained by this strain at 18% NaCl. This report provides genetic information regarding adaptive mechanisms of strain M318 to stress conditions, as well as new knowledge to facilitate the application of this strain as a bacterial cell factory for the production of PHA and ectoine.
Assuntos
Diamino Aminoácidos/biossíntese , Poli-Hidroxialcanoatos/biossíntese , Vibrionaceae/metabolismo , Vias Biossintéticas/genética , Alimentos Fermentados/microbiologia , Microbiologia de Alimentos , Genoma Bacteriano/genética , Plasmídeos , Salinidade , Vibrionaceae/enzimologia , Vibrionaceae/genéticaRESUMO
Collagenase from the Grimontia hollisae strain 1706B (Ghcol) is a zinc metalloproteinase with the zinc-binding motif H492EXXH496. It exhibits higher collagen-degrading activity than the collagenase from Clostridium histolyticum, which is widely used in industry. We previously examined the pH and temperature dependencies of Ghcol activity; Glu493 was thought to contribute acidic pKa (pKe1), while no residue was assigned to contribute alkaline pKa (pKe2). In this study, we introduced nine single mutations at the His or Tyr residues in and near the active site. Our results showed that H412A, H485A, Y497A, H578A and H737A retained the activities to hydrolyze collagen and gelatin, while H426A, H492A, H496A and Y568A lacked them. Purification of active variants H412A, H485A, H578A and H737A, along with inactive variants H492A and H496A, were successful. H412A preferred (7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 to collagen, while H485A preferred collagen to the peptide, suggesting that His412 and His485 are important for substrate specificity. Purification of the active variant Y497A and inactive variants H426A and Y568A were unsuccessful, suggesting that these three residues were important for stability. Based on the reported crystal structure of clostridial collagenase, Tyr568 of Ghcol is suggested to be involved in catalysis and may be the ionizable residue for pKe2.
Assuntos
Colagenases/metabolismo , Histidina/metabolismo , Tirosina/metabolismo , Vibrionaceae/enzimologia , Sequência de Aminoácidos , Catálise , Domínio Catalítico , Colagenases/química , Colagenases/genética , Colagenases/isolamento & purificação , Histidina/química , Histidina/genética , Mutagênese Sítio-Dirigida/métodos , Mutação , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Homologia de Sequência , Relação Estrutura-Atividade , Especificidade por Substrato , Tirosina/química , Tirosina/genética , Vibrionaceae/genéticaRESUMO
Collagenase products are crucial to isolate primary cells in basic research and clinical therapies, where their stability in collagenolytic activity is required. However, currently standard collagenase products from Clostridium histolyticum lack such stability. Previously, we produced a recombinant 74-kDa collagenase from Grimontia hollisae, which spontaneously became truncated to ~60 kDa and possessed no stability. In this study, to generate G. hollisae collagenase useful as a collagenase product, we designed recombinant 62-kDa collagenase consisting only of the catalytic domain, which exhibits high production efficiency. We demonstrated that this recombinant collagenase is stable and active under physiological conditions. Moreover, it possesses higher specific activity against collagen and cleaves a wider variety of collagens than a standard collagenase product from C. histolyticum. Furthermore, it dissociated murine pancreata by digesting the collagens within the pancreata in a dose-dependent manner, and this dissociation facilitated isolation of pancreatic islets with masses and numbers comparable to those isolated using the standard collagenase from C. histolyticum. Implantation of these isolated islets into five diabetic mice led to normalisation of the blood glucose concentrations of all the recipients. These findings suggest that recombinant 62-kDa collagenase from G. hollisae can be used as a collagenase product to isolate primary cells.
Assuntos
Separação Celular/métodos , Colagenases/metabolismo , Proteínas Recombinantes/metabolismo , Vibrionaceae/enzimologia , Animais , Ilhotas Pancreáticas/citologia , Ilhotas Pancreáticas/metabolismo , Camundongos , Vibrionaceae/genéticaRESUMO
Salinivibrio genus is commonly found in salted seafood products. In this study, chitinase produced by Salinivibrio sp. BAO-1801 isolated from salted fermented shrimp was purified and subsequently characterized. The molecular weight of BAO-1801 chitinase was approximately 94.2 kDa by SDS-PAGE analysis. It was classified as a chitinase C based on homology analysis of its N-terminal amino acid residues. This strain BAO-1801 chitinase was then used for synthesis of (GlcNAc)2. Degradation of colloidal chitin and N-acetyl chitooligosaccharides by BAO-1801 chitinase was then analyzed and (GlcNAc)2 was identified as the main product by thin layer chromatography and high-performance liquid chromatography. Effects of temperature and pH on activity and stability of BAO-1801 chitinase were also investigated. Furthermore, this enzyme inhibited fungal growth in a dose-dependent manner. Taken together, these results suggest that this Salinivibrio or its chitinase can be used for the enzymatic degradation of chitin to produce chitobiose in industrial process.
Assuntos
Antifúngicos/farmacologia , Proteínas de Bactérias/fisiologia , Quitinases/fisiologia , Dissacarídeos/biossíntese , Microbiologia de Alimentos , Vibrionaceae/enzimologia , Sequência de Aminoácidos , Antifúngicos/química , Antifúngicos/isolamento & purificação , Proteínas de Bactérias/química , Proteínas de Bactérias/isolamento & purificação , Quitina/metabolismo , Quitinases/química , Quitinases/isolamento & purificação , Quitinases/metabolismo , DNA Bacteriano/genética , Dissacarídeos/metabolismo , Estabilidade Enzimática , Fungos/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Peso Molecular , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Especificidade por Substrato , Temperatura , Vibrionaceae/classificação , Vibrionaceae/genéticaRESUMO
Organic solvents tend to strip water from protein and thereby disrupt non-covalent forces and decrease enzyme activity and stability. In the present study, we have replaced the surface charge residues in Salinivibrio zinc-metalloprotease (SVP) with hydrophobic ones (E12V, D22I, D24A and D310I) in order to study the effects of surface hydrophobicity with hydrophobic strength of organic solvents. Compared to SVP, D24A exhibited an increase in kcat and catalytic efficiency and a reduction in thermal inactivation rate in aqueous solvent. Structural studies indicated that the replacement of surface charge residues with hydrophobic residues would not induce conformational changes. C50 value (the value of solvent concentration where 50% of enzyme activity remains), ki (irreversible thermoinactivation rate), and kinetic parameters of E12V, D22I, and D24A were higher in isopropanol and n-propanol. D24A is found to be the most efficient mutant for its remarkable decrease in ki value in the presence of isopropanol and n-propanol and a reduction in ki value in the presence of dimethylformamide (DMF) and methanol. C50 value in this variant was increased about 1.2% in DMF, 2% in methanol and isopropanol and 2.5% in n-propanol. Results revealed that, there was a correlation between surface hydrophobicity of SVP and hydrophobic strength of organic solvents.
Assuntos
Metaloendopeptidases/química , Conformação Proteica , Solventes/química , Vibrionaceae/enzimologia , Catálise , Estabilidade Enzimática , Interações Hidrofóbicas e Hidrofílicas , Cinética , Metaloendopeptidases/genética , Propriedades de Superfície , Vibrionaceae/química , Água/química , Zinco/químicaRESUMO
The collagenase produced by a gram-negative bacterium Grimontia hollisae strain 1706B (Ghcol) degrades collagen more efficiently than that produced by a gram-positive bacterium Clostridium histolyticum (Chcol), which is currently the most widely used collagenase in industry [Teramura et al. (Cloning of a novel collagenase gene from the gram-negative bacterium Grimotia (Vibrio) hollisae 1706B and its efficient expression in Brevibacillus choshinensis. J Bacteriol 2011;193:3049-3056)]. Here, we compared the Ghcol and Chcol activities using two synthetic substrates. In the hydrolysis of (7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Pro-L-Leu-Gly-L-Leu-[N3-(2, 4-dinitrophenyl)-L-2, 3-diaminopropioyl]-L-Ala-L-Arg-NH2, Ghcol exhibited 350-fold higher activity than Chcol in the absence of CaCl2 and NaCl. The Ghcol activity markedly decreased with increasing concentrations of buffer, CaCl2 or NaCl, while the Chcol activity did not, suggesting that the Ghcol activity was sensitive to solvent components. In the hydrolysis of N-[3-(2-furyl)acryloyl]-L-Leu-Gly-L-Pro-Ala, Ghcol exhibited 16-fold higher activity than Chcol in the absence of CaCl2 and NaCl, and both enzyme activities did not decrease with increasing concentrations of buffer, CaCl2 or NaCl. pH dependences of activity revealed that the ionizable group responsible for acidic pKe may be Glu for Ghcol and Chcol, while that for alkaline pKe may be His for Ghcol and Tyr for Chcol. These striking differences suggest that the catalytic mechanism of Ghcol might be considerably different from that of clostridial collagenases.
Assuntos
Clostridium/enzimologia , Colagenases/metabolismo , Fragmentos de Peptídeos/metabolismo , Vibrionaceae/enzimologia , Cloreto de Cálcio/química , Clostridium/metabolismo , Concentração de Íons de Hidrogênio , Hidrólise , Fragmentos de Peptídeos/síntese química , Fragmentos de Peptídeos/química , Cloreto de Sódio/química , Temperatura , Vibrionaceae/metabolismoRESUMO
Glycosylphosphatidylinositol transamidase (GPI-T) catalyzes the post-translational addition of the GPI anchor to the C-terminus of some proteins. In most eukaryotes, Gpi8, the active site subunit of GPI-T, is part of a hetero-pentameric complex containing Gpi16, Gaa1, Gpi17, and Gab1. Gpi8, Gaa1, and Gpi16 co-purify as a heterotrimer from Saccharomyces cerevisiae, suggesting that they form the core of the GPI-T. Details about the assembly and organization of these subunits have been slow to emerge. We have previously shown that the soluble domain of S. cerevisiae Gpi8 (Gpi823-306) assembles as a homodimer, similar to the caspases with which it shares weak sequence homology (Meitzler, J. L. et al., 2007). Here we present the characterization of a complex between the soluble domains of Gpi8 and Gaa1. The complex between GST-Gpi823-306 (α) and His6-Gaa150-343 (ß) was characterized by native gel analysis and size exclusion chromatography (SEC) and results are most consistent with an α2ß2 stoichiometry. These results demonstrate that Gpi8 and Gaa1 interact specifically without a requirement for other subunits, bring us closer to determining the stoichiometry of the core subunits of GPI-T, and lend further credence to the hypothesis that these three subunits assemble into a dimer of a trimer.
Assuntos
Aminoaciltransferases/química , Glicoproteínas de Membrana/química , Proteínas de Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/química , Motivos de Aminoácidos , Aminoaciltransferases/genética , Aminoaciltransferases/metabolismo , Sítios de Ligação , Clonagem Molecular , Escherichia coli/genética , Escherichia coli/metabolismo , Expressão Gênica , Cinética , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Modelos Moleculares , Ligação Proteica , Conformação Proteica em alfa-Hélice , Conformação Proteica em Folha beta , Domínios e Motivos de Interação entre Proteínas , Multimerização Proteica , Estrutura Terciária de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae/enzimologia , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Solubilidade , Homologia Estrutural de Proteína , Especificidade por Substrato , Vibrionaceae/química , Vibrionaceae/enzimologiaRESUMO
Chitinolytic microorganisms secrete a range of chitin modifying enzymes, which can be exploited for production of chitin derived products or as fungal or pest control agents. Here, we explored the potential of 11 marine bacteria (Pseudoalteromonadaceae, Vibrionaceae) for chitin degradation using in silico and phenotypic assays. Of 10 chitinolytic strains, three strains, Photobacterium galatheae S2753, Pseudoalteromonas piscicida S2040 and S2724, produced large clearing zones on chitin plates. All strains were antifungal, but against different fungal targets. One strain, Pseudoalteromonas piscicida S2040, had a pronounced antifungal activity against all seven fungal strains. There was no correlation between the number of chitin modifying enzymes as found by genome mining and the chitin degrading activity as measured by size of clearing zones on chitin agar. Based on in silico and in vitro analyses, we cloned and expressed two ChiA-like chitinases from the two most potent candidates to exemplify the industrial potential.
Assuntos
Antifúngicos/isolamento & purificação , Quitina/metabolismo , Quitinases/metabolismo , Pseudoalteromonas/enzimologia , Vibrionaceae/enzimologia , Antifúngicos/farmacologia , Biologia Marinha , Pseudoalteromonas/genética , Vibrionaceae/genéticaRESUMO
OBJECTIVES: To improve the production and activity of an alkaline zinc metalloprotease from Salinivibrio proteolyticus in response to ZnSO4 (ionic and nanoparticle forms) and low intensity direct electric current (LIDC). RESULTS: A DC of 50 µA for 10 min increased enzyme production from 35 to 53 U ml(-1) when applied to the stationary phase bacterial cells. Zn(2+) improved enzyme production better than zinc nanoparticles (52 vs. 43.5 U ml(-1)). Zinc nanoparticles (0.5 mM) added to an enzyme reaction mixture containing casein (0.65 %) and 20 mM Tris/HCl buffer (pH 8) improved enzyme activity more than Zn(2+) (42 vs. 36 U ml(-1)). CONCLUSION: LIDC exposure (50 µA, 10 min) to the stationary phase bacterial cells increases metalloprotease production in Salinivibrio. A low concentration of zinc nanoparticles (0.5 mM) increases maximum enzyme activity.
Assuntos
Nanopartículas Metálicas/química , Metaloproteases/metabolismo , Vibrionaceae/enzimologia , Zinco/químicaRESUMO
An extracellular protease was purified from a novel moderately halophilic bacterium Salinivibrio sp. strain MS-7 by the combination of an acetone precipitation (40-80 %) step and a DEAE-cellulose anion exchange column chromatography. Kinetic parameters of the enzyme exhibited V(max) and K(m) of 130 U/mg and 1.14 mg/ml, respectively, using casein as a substrate. The biochemical properties of the enzyme revealed that the 21-kDa protease had a temperature and pH optimum of 50 °C and 8.0, respectively. The enzyme was strongly inhibited by phenylmethylsulfonyl fluoride, Pefabloc SC, chymostatin, and also EDTA, indicating that it belongs to the class of serine metalloproteases. Interestingly, Ba(2+) and Ca(2+) (2 mM) strongly enhanced the enzyme activity, while Fe(2+) and Mg(2+) activated moderately and Zn(2+), Ni(2+), and Hg(2+) decreased the enzyme activity. The effect of organic solvents with different logP on the purified protease revealed complete stability in toluene, ethyl acetate, chloroform, and n-hexane at 10 and 50 % (v/v) and moderate stability even in 50 % of DMSO and ethanol. The behavior of the MS-7 protease in three imidazolium-based ionic liquids exhibited suitable activity in these green solvent systems, especially in 1-hexyl-3-methylimidazolium hexafluorophosphate ([C(6)MIM][PF(6)]). Comparison of the purified protease with other previously reported proteases suggests that strain MS-7 secrets a novel organic solvent-tolerant protease with outstanding activity in organic solvents and imidazolium-based ionic liquids, which could be applied in low water synthetic section of industrial biotechnology.
Assuntos
Metaloproteases/metabolismo , Serina Proteases/metabolismo , Solventes/farmacologia , Caseínas/metabolismo , Cromatografia DEAE-Celulose , Estabilidade Enzimática/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Imidazóis/farmacologia , Líquidos Iônicos/farmacologia , Cinética , Metaloproteases/isolamento & purificação , Metais/farmacologia , Desnaturação Proteica , Serina Proteases/isolamento & purificação , Inibidores de Serina Proteinase/farmacologia , Vibrionaceae/enzimologiaRESUMO
In this work, SVP2 from Salinivibrio proteolyticus strain AF-2004, a zinc metalloprotease with suitable biotechnological applications, was cloned for expression at high levels in Escherichia coli with the intention of changing culture conditions to generate a stable extracellular enzyme extract. The complete ORF of SVP2 gene was heterologously expressed in E. coli BL21 (DE3) by using pQE-80L expression vector system. In initial step, the effect of seven factors include: incubation temperature, peptone and yeast extract concentration, cell density (OD600) before induction, inducer (IPTG) concentration, induction time, and Ca(2+) ion concentrations on extracellular recombinant SVP2 expression and stability were investigated. The primary results revealed that the IPTG concentration, Ca(2+) ion concentration and induction time are the most important effectors on protease secretion by recombinant E. coli BL21. Central composite design experiment in the following showed that the maximum protease activity (522 U/ml) was achieved in 0.0089 mM IPTG for 24h at 30 °C, an OD600 of 2, 0.5% of peptone and yeast extract, and a Ca(2+) ion concentration of 1.3 mM. The results exhibited that the minimum level of IPTG concentration along with high cell density and medium level of Ca(2+) with prolonged induction time provided the best culture condition for maximum extracellular production of heterologous protease SVP2 in E. coli expression system.
Assuntos
Escherichia coli/genética , Metaloproteases/biossíntese , Escherichia coli/enzimologia , Metaloproteases/química , Metaloproteases/genética , Modelos Estatísticos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Reprodutibilidade dos Testes , Projetos de Pesquisa , Vibrionaceae/enzimologia , Vibrionaceae/genéticaRESUMO
Carbapenems were the last ß-lactams retaining near-universal anti-Gram-negative activity, but carbapenemases are spreading, conferring resistance. New Delhi metallo-ß-lactamase (NDM) enzymes are the latest carbapenemases to be recognized and since 2008 have been reported worldwide, mostly in bacteria from patients epidemiologically linked to the Indian subcontinent, where they occur widely in hospital and community infections, and also in contaminated urban water. The main type is NDM-1, but minor variants occur. NDM enzymes are present largely in Enterobacteriaceae, but also in non-fermenters and Vibrionaceae. Dissemination predominantly involves transfer of the blaNDM-1 gene among promiscuous plasmids and clonal outbreaks. Bacteria with NDM-1 are typically resistant to nearly all antibiotics, and reliable detection and surveillance are crucial.
Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Carbapenêmicos/farmacologia , Enterobacteriaceae/efeitos dos fármacos , Enterobacteriaceae/enzimologia , Infecções por Bactérias Gram-Negativas/microbiologia , Resistência beta-Lactâmica , beta-Lactamases/genética , beta-Lactamases/metabolismo , Surtos de Doenças , Enterobacteriaceae/isolamento & purificação , Transferência Genética Horizontal , Humanos , Plasmídeos , Pseudomonas/efeitos dos fármacos , Pseudomonas/enzimologia , Pseudomonas/isolamento & purificação , Vibrionaceae/efeitos dos fármacos , Vibrionaceae/enzimologia , Vibrionaceae/isolamento & purificaçãoRESUMO
In order to clarify the impact of Ca-binding sites (Ca1 and 2) on the conformational stability of neutral proteases (NPs), we have analyzed the thermal, pH and organic solvent stability of a NP variant, V189P/A195E/G203D/A268E (Q-mutant), from Salinovibrio proteolyticus. This mutant has shown to bind calcium more tightly than the wild-type (WT) at Ca1 and to possess Ca2. Q-mutant was resisted against autolysis, thermoinactivation and pH denaturation in a Ca-dependent manner and exhibited better activity in organic solvents compared to the WT enzyme. These results imply that Ca1 and Ca2 are important for the conformational stability of NPs.
Assuntos
Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Cálcio/metabolismo , Peptídeo Hidrolases/química , Peptídeo Hidrolases/metabolismo , Solventes/química , Vibrionaceae/enzimologia , Proteínas de Bactérias/genética , Sítios de Ligação , Cálcio/química , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , Peptídeo Hidrolases/genética , Conformação Proteica , TemperaturaRESUMO
Stabilizing an enzyme while improving its activity may be difficult with respect to a general trade off relation between stability and function at the level of individual mutations. We have used site-directed mutagenesis to investigate the possibility of parallel improvements of thermostability and activity in the neutral protease from Salinovibrio proteolyticus. Four out of seven point mutations are able to promote both activity and thermostability individually and combinedly. The catalytic efficiency (k(cat)/K(m)) of four-amino acid substituted variant (quadruple mutant) at 60 degrees C is 18-fold higher than wild type, whereas at optimum temperature is almost 50-fold higher. Quadruple mutant shows an upward shift of 14 degrees C in the temperature optimum, and a 20-, 24-, 7- and 5-fold increase in half-life at 60, 65, 70 and 75 degrees C, respectively, as a result of enhanced calcium binding. Theoretical studies have provided evidences that hinge-bending angle is reduced by amino acid substitutions. Finally, we conclude that the extended surface region between residues 187-228, which involves three out of four beneficial mutations, influences the hinge angle which is determinant for catalysis and also involves the structural calcium which is critical for stability.
Assuntos
Proteínas de Bactérias , Peptídeo Hidrolases , Vibrionaceae/enzimologia , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Cálcio , Ativação Enzimática , Estabilidade Enzimática , Cinética , Modelos Moleculares , Mutagênese Sítio-Dirigida , Peptídeo Hidrolases/química , Peptídeo Hidrolases/genética , Peptídeo Hidrolases/metabolismo , Mutação Puntual , Conformação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Temperatura , Vibrionaceae/genéticaRESUMO
Salinivibrio zinc-metalloprotease (SVP) is an enzyme which was isolated from Salinivibrio proteolyticus, a moderately halophilic species from a hypersaline lake in Iran. A195E and G203D mutants were constructed to increase polarity near the active site in order to preserve the hydration layer against organic solvents [dimethylformamide (DMF), methanol, isopropanol and n-propanol]. A268P was constructed to stabilize a surface loop far from the active site and A195E/A268P was constructed to investigate the combined effects of these two mutations. Results showed that relative C(50) values of A195E increased to approximately 26 and 11% in DMF and methanol whereas an increase of approximately 32 and 41% was observed in the presence of isopropanol and n-propanol. The irreversible thermoinactivation rate (k(i)) for A195E was estimated to be 60 and 130 (x10(-3) min(-1)) in the presence of DMF and n-propanol, respectively, while k(i) for SVP was 90 and 190 (x10(-3) min(-1)). G203D exhibited similar k(i) as A195E in the presence of methanol and isopropanol, but the calculated k(i) in the presence of DMF and n-propanol was 70 and 160 (x10(-3) min(-1)), respectively. A268P and A268P/A195E variants marginally increased the thermoresistance of the enzyme in this condition.
Assuntos
Metaloproteases/química , Domínio Catalítico , Estabilidade Enzimática/efeitos dos fármacos , Temperatura Alta , Metaloproteases/efeitos dos fármacos , Metaloproteases/genética , Metaloproteases/metabolismo , Modelos Moleculares , Mutagênese Sítio-Dirigida , Solventes/farmacologia , Vibrionaceae/enzimologiaRESUMO
Chitin deacetylases, occurring in marine bacteria, several fungi and a few insects, catalyze the deacetylation of chitin, a structural biopolymer found in countless forms of marine life, fungal cell and spore walls as well as insect cuticle and peritrophic matrices. The deacetylases recognize a sequence of four GlcNAc units in the substrate, one of which undergoes deacetylation: the resulting chitosan has a more regular deacetylation pattern than a chitosan treated with hot NaOH. Nevertheless plain chitin is a poor substrate, but glycolated, reprecipitated or depolymerized chitins are good ones. The marine Vibrio sp. colonize the chitin particles and decompose the chitin thanks to the concerted action of chitinases and deacetylases, otherwise they could not tolerate chitosan, a recognized antibacterial biopolymer. In fact, chitosan is used to prevent infections in fishes and crustaceans. Considering that chitin deacetylases play very important roles in the biological attack and defense systems, they may find applications for the biological control of fungal plant pathogens or insect pests in agriculture and for the biocontrol of opportunistic fungal human pathogens.
Assuntos
Amidoidrolases , Proteínas de Bactérias , Proteínas Fúngicas , Proteínas de Insetos , Amidoidrolases/antagonistas & inibidores , Amidoidrolases/química , Amidoidrolases/genética , Amidoidrolases/fisiologia , Animais , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/fisiologia , Quitina/química , Quitina/metabolismo , Quitina/farmacologia , Quitosana/química , Quitosana/metabolismo , Quitosana/farmacologia , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Proteínas Fúngicas/fisiologia , Fungos/enzimologia , Fungos/patogenicidade , Humanos , Proteínas de Insetos/química , Proteínas de Insetos/genética , Proteínas de Insetos/fisiologia , Insetos/enzimologia , Controle Biológico de Vetores/métodos , Vibrionaceae/enzimologiaRESUMO
Four strains isolated from cultured Manila clam, Ruditapes philippinarum, in the north-western coast of Spain were characterized phenotypically and genotypically. Phylogenetic analyses based on the 16S rRNA gene sequences indicated that these bacteria were closely related to Aliivibrio wodanis, Aliivibrio salmonicida, Aliivibrio fischeri and Aliivibrio logei with sequence similarities between 98.1 and 96.0 %. Phylogenetic analysis based on the RNA polymerase alpha chain (rpoA), RecA protein (recA), the alpha-subunit of bacterial ATP synthase (atpA) and the uridine monophosphate (UMP) kinase (pyrH) genes and fluorescent amplified fragment length polymorphism experiments clearly showed that these novel isolates form a tight genomic group different from any currently known Aliivibrio species. On the basis of phylogenetic analysis and phenotypic data, the four strains represent a novel taxon, for which the name Aliivibrio finisterrensis sp. nov. is proposed. Several phenotypic features were revealed that discriminate A. finisterrensis from other Aliivibrio species. The type strain is CMJ 11.1(T) (=CECT 7228(T)=LMG 23869(T)).