RESUMO
1. The final enzymes in the biosynthesis of aldosterone and cortisol are by the cytochrome P450 CYP11B2 and CYP11B1, respectively. The enzymes are 93% homologous at the amino acid level and specific antibodies have been difficult to generate. 2. Mice and rats were immunized with multiple peptides conjugated to various immunogenic proteins and monoclonal antibodies were generated. The only peptide sequences that generated specific antibodies were amino acids 41-52 for the CYP11B2 and amino acids 80-90 for the CYP11B1 enzyme. 3. The mouse monoclonal CYP11B2-41 was specific and sensitive for use in western blots and produced specific staining of the zona glomerulosa of normal adrenal glands. The rat monoclonal CYP11B1-80 also detected a single band by western blot and detected only the zona fasciculata. Triple immunofluorescence of the adrenal demonstrated that the CYP11B1 and the CYP11B2 did not co-localize, while as expected the CYP11B1 co-localized with the 17α-hydroxylase.
Assuntos
Anticorpos Monoclonais/biossíntese , Citocromo P-450 CYP11B2/imunologia , Peptídeos/imunologia , Esteroide 11-beta-Hidroxilase/imunologia , Zona Fasciculada/ultraestrutura , Zona Glomerulosa/ultraestrutura , Adulto , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/isolamento & purificação , Citocromo P-450 CYP11B2/metabolismo , Humanos , Imuno-Histoquímica/métodos , Recém-Nascido , Camundongos , Dados de Sequência Molecular , Peptídeos/administração & dosagem , Peptídeos/síntese química , Ratos , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Esteroide 11-beta-Hidroxilase/metabolismo , Esteroide 17-alfa-Hidroxilase/imunologia , Esteroide 17-alfa-Hidroxilase/metabolismo , Zona Fasciculada/imunologia , Zona Fasciculada/metabolismo , Zona Glomerulosa/imunologia , Zona Glomerulosa/metabolismoRESUMO
Prolactin is a hormone with a multidirectional proinflammatory action. It has an anti-apoptotic effect, enhances proliferative response to antigens and mitogens, as well as enhances the production of immunoglobulins and autoantibodies. Increased prolactin levels are commonly observed in various organ and multi-organ specific autoimmune diseases. In our article, we report a case of a woman who developed progression of autoimmune thyroid disorder and developed insufficiency of the zona glomerulosa when her prolactin levels were increased. A normalization of plasma prolactin levels by quinagolide and replacement of risperidone with aripiprazole improved her clinical condition. Our study suggests that, in some patients, hyperprolactinemia may predispose to the development and progression of autoimmune disorders of endocrine glands.
Assuntos
Hiperprolactinemia/complicações , Hiperprolactinemia/imunologia , Tireoidite Autoimune/complicações , Tireoidite Autoimune/imunologia , Aminoquinolinas/administração & dosagem , Progressão da Doença , Agonistas de Dopamina/administração & dosagem , Feminino , Humanos , Hiperprolactinemia/tratamento farmacológico , Prolactina/sangue , Prolactina/imunologia , Adulto Jovem , Zona Glomerulosa/imunologiaRESUMO
Human carbonic anhydrase isoenzymes I and II (HCA I and II) were purified from human erythrocytes by inhibitor affinity chromatography and ion-exchange chromatography. These isoenzymes were then located in the human adrenal gland using specific polyclonal antisera raised in rabbits and specific detection by immunohistochemical techniques. Both HCA II and I were located in the zona glomerulosa cells, although the staining for HCA I was faint. The cells of the zona fasciculata and the zona reticularis failed to stain with either antiserum. Control stainings with preimmune or anti-HCA VI sera were negative. The presence of HCA II and I in the zona glomerulosa cells may be linked to regulation of the biosynthesis or secretion of mineralocorticoids.
