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1.
Molecules ; 26(6)2021 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-33801151

RESUMO

Severe acute respiratory syndrome coronavirus (SARS-CoV-2) disease is a global rapidly spreading virus showing very high rates of complications and mortality. Till now, there is no effective specific treatment for the disease. Aloe is a rich source of isolated phytoconstituents that have an enormous range of biological activities. Since there are no available experimental techniques to examine these compounds for antiviral activity against SARS-CoV-2, we employed an in silico approach involving molecular docking, dynamics simulation, and binding free energy calculation using SARS-CoV-2 essential proteins as main protease and spike protein to identify lead compounds from Aloe that may help in novel drug discovery. Results retrieved from docking and molecular dynamics simulation suggested a number of promising inhibitors from Aloe. Root mean square deviation (RMSD) and root mean square fluctuation (RMSF) calculations indicated that compounds 132, 134, and 159 were the best scoring compounds against main protease, while compounds 115, 120, and 131 were the best scoring ones against spike glycoprotein. Compounds 120 and 131 were able to achieve significant stability and binding free energies during molecular dynamics simulation. In addition, the highest scoring compounds were investigated for their pharmacokinetic properties and drug-likeness. The Aloe compounds are promising active phytoconstituents for drug development for SARS-CoV-2.


Assuntos
Aloe/química , Antivirais/análise , Antivirais/química , Desenvolvimento de Medicamentos , Glicoproteína da Espícula de Coronavírus/antagonistas & inibidores , Antivirais/metabolismo , Antivirais/farmacocinética , Biologia Computacional , Descoberta de Drogas/métodos , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/química , Compostos Fitoquímicos/metabolismo , Compostos Fitoquímicos/farmacocinética , Ligação Proteica , Glicoproteína da Espícula de Coronavírus/metabolismo , Termodinâmica
2.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 52(2): 241-247, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33829698

RESUMO

Objective: To synthesize three kinds of metal complexes of aloe-emodin and compare the antioxidant activities of the ligands and the complexes. Methods: Three kinds of aloe emodin metal complex, the aloe-emodin-iron (Ⅱ), the aloe-emodin-copper (Ⅱ) and the aloe-emodin-magnesium (Ⅱ) complexes, were synthesized by dissolving and stirring in anhydrous ethanol solvent, and their structures were characterized. The Fe 2+-H 2O 2-methylene blue method, the diphenyl bitter hydrazine radical method (DPPH method) and other assays were used to determine the clearance effect of ligands and complexes on superoxide radicals (O 2 -•), hydroxyl radicals (•OH) and phenyl bitter hydrazine radical (DPPH•). Results: Three kinds of aloe emodin metal complex, the aloe-emodin-iron (Ⅱ), the aloe-emodin-copper (Ⅱ) and the aloe-emodin-magnesium (Ⅱ) complexes, were successfully synthesized. According to the results of structural characterization, we speculated that the aloe-emodin metal complexes were formed at the site between the two molecules of aloe-emodin and one molecule of metal ions (Fe 2+, Mg 2+, Cu 2+) via the 9 th carbonyl and 8 th hydroxyl groups of the aloe-emodin molecules. Both the complex and the ligand have clearance effects on three kinds of free radicals, and the complex showed stronger effects than its ligand ( P<0.05). Conclusion: Coordination of aloe-emodin with metal ions, such as Fe 2+, Cu 2+, and Mg 2+, could enhance the antioxidant activity of the ligand itself.


Assuntos
Aloe , Complexos de Coordenação , Emodina , Antraquinonas , Antioxidantes
3.
Carbohydr Polym ; 261: 117874, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33766361

RESUMO

Despite the well-known health benefits of aloe polysaccharide (APs), little is known about how APs modulate the gut microbiota and the relationship between microbiota and SCFAs. Here, APs was extracted by ultrasound extraction. FT-IR and glycosidic linkage type analysis showed that a major part of APs consisted of → 4)-ß-Manp-(1 → residues with acetyl groups. APs supplementation to mice prominently boosted SCFAs-producing Bacteroides and Parabacteria in the feces. On the other hand, it decreased the abundance of Firmicutes and Clostridium. A positive correlation between microbiota and SCFAs was revealed, with Parabacteria and Clostridium being the key microbiota to significantly promote SCFAs. APs promoted the fructose and mannose metabolism by upregulating the gene expression of 17 enzymes, containing fructose-bisphosphate aldolase [EC:4.1.2.13]. Our findings highlight SCFAs-producing Parabacteria were primary degrader of APs, and APs may have prebiotic effects on gut microbiota.


Assuntos
Aloe/química , Microbioma Gastrointestinal/efeitos dos fármacos , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Prebióticos , Animais , Fracionamento Químico , Ácidos Graxos Voláteis/análise , Fezes/química , Fezes/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Polissacarídeos/análise , Prebióticos/análise , Espectroscopia de Infravermelho com Transformada de Fourier
4.
Environ Sci Pollut Res Int ; 28(12): 15565-15573, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33580365

RESUMO

The objective of this research was to find out the potential of bioscouring using xylano-pectinolytic enzymes, for degumming of aloe fibers. Bioscouring was optimized with 1 : 20 fiber to buffer ratio, using 10 IU xylanase and 3.2 IU pectinase in 50 mM buffer (pH 8.5), EDTA (3 mM), and Tween 80 (1%), at 50°C temperature with agitation rate of 50 rpm and treatment period of 60 min. Enzymatic treatment of aloe fibers increased brightness and whiteness by 55.67% and 24.88%, respectively and decreased yellowness by 44.11% as compared to alkaline fiber scouring, thereby replacing chemical scouring completely. Additionally, the pretreatment of aloe fibers with enzymes resulted in a 50% less consumption of bleaching chemicals with similar optical properties as obtained by 100% bleaching. This is the first report showing the eco-friendly bioscouring approach of aloe fibers, using enzymes produced concurrently from a bacterial isolate.


Assuntos
Aloe , Conservação dos Recursos Naturais , Poligalacturonase , Tecnologia , Temperatura , Estados Unidos
5.
Int J Mol Sci ; 22(4)2021 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-33567756

RESUMO

Aloe vera (AV), a succulent plant belonging to the Liliaceae family, has been widely used for biomedical and pharmaceutical application. Its popularity stems from several of its bioactive components that have anti-oxidant, anti-microbial, anti-inflammatory and even immunomodulatory effects. Given such unique multi-modal biological impact, AV has been considered as a biomaterial for regenerative medicine and tissue engineering applications, where tissue repair and neo-angiogenesis are vital. This review outlines the growing scientific evidence that demonstrates the advantage of AV as tissue engineering scaffolds. We particularly highlight the recent advances in the application of AV-based scaffolds. From a tissue engineering perspective, it is pivotal that the implanted scaffolds strike an appropriate foreign body response to be well-accepted in the body without complications. Herein, we highlight the key cellular processes that regulate the foreign body response to implanted scaffolds and underline the immunomodulatory effects incurred by AV on the innate and adaptive system. Given that AV has several beneficial components, we discuss the importance of delving deeper into uncovering its action mechanism and thereby improving material design strategies for better tissue engineering constructs for biomedical applications.


Assuntos
Aloe/química , Materiais Biocompatíveis/química , Imunomodulação , Medicina Regenerativa , Tecidos Suporte/química , Cicatrização , Animais , Humanos
6.
Cryobiology ; 99: 40-45, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33508264

RESUMO

Addressing the establishment of biobanks for the conservation of wild hystricomorph rodents' germplasm, we verified the effects of different extenders and distinct concentrations of non-permeant cryoprotectants on the sperm parameters of Spix's yellow-toothed cavies. Nine testis-epididymis complexes were used for sperm collection by retrograde washing using Tris or a powdered coconut water extender (ACP®-116c). Spermatozoa were diluted and frozen with the same extenders supplemented with egg yolk or Aloe vera at a 10% or 20% concentration. After recovery and cryopreservation, all samples were evaluated for sperm kinetic parameters, morphology, membrane integrity, osmotic response, and sperm-binding capability using an egg yolk perivitelline membrane assay. After recovery, no differences were observed between Tris and ACP®-116c that provided 515.4 × 106 sperm/mL and 561.6 × 106 sperm/mL, presenting >65% motile sperm, respectively. After cryopreservation, most effective preservation of sperm kinetic parameters (68.1 ± 5.9% motile sperm) and membrane integrity (48.2 ± 7.4%) was provided by Tris extender supplemented with 10% egg yolk. However, both extenders supplemented with any concentration of egg yolk or Aloe vera presented similar preservation of osmotic response and sperm-binding ability after cryopreservation. In summary, we suggest the use of a Tris extender supplemented of 10% egg yolk for cryopreservation of Spix's yellow-toothed cavy epidydimal sperm.


Assuntos
Aloe , Preservação do Sêmen , Animais , Cocos , Criopreservação/métodos , Crioprotetores/farmacologia , Gema de Ovo , Epididimo , Cobaias , Masculino , Análise do Sêmen , Preservação do Sêmen/veterinária , Motilidade Espermática , Espermatozoides , Água
7.
J Sci Food Agric ; 101(2): 414-423, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32643805

RESUMO

BACKGROUND: Aloe vera is a popular medicinal plant used widely by the cosmetic, pharmaceutical, and food industries. The A. vera leaf gel, which is used mostly for its positive effects on human health, contains over 75 different bioactive compounds, including aloin. Aloin is a toxic compound, and its content in A. vera leaf gel products depends on the different cultivation conditions and especially on leaf processing. RESULTS: In this study, A. vera leaf gel products, varied in terms of leaf processing, were analyzed using liquid chromatography for their aloin content, their antioxidant activity by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) radical cation (ABTS·+ ) and the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH· ) antioxidant activity assays and their toxicity against Aliivibrio fisheri and SH-SY5Y cells. In the samples processed with industrial methods and in those filtered in the lab, the content of aloin was found below the limit (0.1 mg L-1 ) of the EU legislation however, the unprocessed and unfiltered samples were found to contain more than 10 mg L-1 . Antioxidant activity was estimated to vary from 1.64 to 9.21 µmol Trolox mL-1 for DPPH· and from 0.73 to 5.14 µmol Trolox mL-1 for ABTS·+ . Toxicity values on A. fisheri, expressed as the concentration at 50% loss of initial luminescence, ranged from 0.03 to 0.09 mg mL-1 . The cytotoxic study indicated that aloin A at low concentrations (1 and 10 µg mL-1 ) protects SH-SY5Y cells from toxicity induced by hydrogen peroxide. CONCLUSIONS: Consequently, the filtration process of A. vera leaf gels, either laboratory or industrial, resulted in aloin A content below the EU legislation detection limits. © 2020 Society of Chemical Industry.


Assuntos
Aloe/química , Antioxidantes/análise , Emodina/análogos & derivados , Preparações de Plantas/análise , Aliivibrio fischeri/efeitos dos fármacos , Antioxidantes/toxicidade , Linhagem Celular , Cromatografia Líquida de Alta Pressão , Emodina/análise , Emodina/toxicidade , Grécia , Humanos , Extratos Vegetais/análise , Extratos Vegetais/toxicidade , Folhas de Planta/química , Preparações de Plantas/toxicidade
8.
Braz. dent. sci ; 24(1): 1-7, 2021. tab, ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1145548

RESUMO

Though aloe vera extract, green tea extract and coriander oil are proven antimicrobial agents, very little information is available regarding its effects on oral bacteria, Streptococcus mutans, which is responsible for initiating caries and Enterococcus faecalis, responsible for failure of root canal treatment. Objective: To find the antimicrobial activity of aloe vera extract, black tea extract and coriander oil against S. mutans and E. faecalis. Materials and Methods: The agar well diffusion method was used to determine the antibacterial activity of Aloe vera extract, black tea extract and coriander oil. Different concentration of prepared plant extracts and coriander seed oil (50 & 100 µl) was incorporated into the wells and the plates containing S. mutans and E. faecalis were incubated at 37 °C for 24 h. The antibiotic (amoxicillin 30 µl) was used as positive control. Zone Of Inhibition (ZOI) was recorded in each plate. Results: For S. mutans, the maximum ZOI was created by coriander oil with a diameter of 25.00±0.58 mm at 50 µl and for E. faecalis, maximum ZOI was created by aloe vera extract 16.00±0.58 mm at 100 µl concentration which were far better than the control: amoxicillin 30 µl concentration. Conclusion: The extracts of Aloe vera, black tea and coriander oil, showed significant activity against the investigated microbial strains, Streptococcus mutans and Enterococcus faecalis which further helps in the development of new topical agents that help in reducing the numbers of these organisms present in the oral cavity. (AU)


Embora o extrato de aloe vera, extrato de chá verde e óleo de coentro sejam agentes antimicrobianos comprovados, há pouca informação disponível sobre seus efeitos nas bactérias orais, Streptococcus mutans, que é responsável por iniciar cáries e Enterococcus faecalis, responsável pela falha do tratamento de canal radicular. Objetivo: Avaliar a atividade antimicrobiana do extrato de aloe vera, extrato de chá preto e óleo de coentro contra S. mutans e E. faecalis. Materiais e Métodos: O método de difusão em agar foi usado para determinar a atividade antibacteriana do extrato de Aloe vera, extrato de chá preto e óleo de coentro. Diferentes concentrações dos extratos de plantas e óleo de semente de coentro (50 e 100 µl) foram preparados e colocados nos poços e nas placas contendo S. mutans e E. faecalis e foram incubadas a 37°C por 24 h. O antibiótico (amoxicilina 30 µl) foi utilizado como controle positivo. A zona de inibição (ZOI) foi registrada em cada placa. Resultados: Para S. mutans, a ZOI máxima foi obtida com o óleo de coentro com um diâmetro de 25,00 ± 0,58 mm a 50 µl e para E. faecalis, a ZOI máxima foi obtiada pelo extrato de aloe vera 16,00 ± 0,58 mm na concentração de 100 µl, as quais foram melhores do que o controle: concentração de 30 µl de amoxicilina. Conclusão: Os extratos de Aloe vera, chá preto e óleo de coentro apresentaram atividade significativa contra as cepas microbianas investigadas, Streptococcus mutans e Enterococcus faecalis auxiliando no desenvolvimento de novos agentes tópicos visando a redução do número desses organismos presentes no cavidade oral. (AU)


Assuntos
Streptococcus mutans , Chá , Enterococcus faecalis , Aloe , Microbiota
9.
Water Sci Technol ; 82(11): 2446-2459, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33339798

RESUMO

Aloe vera is an important commodity plant which has been traditionally used for the treatment of various diseases. This study investigated the use of extracted bioflocculant from Aloe vera for the treatment of textile wastewater. The bioflocculant was extracted, purified and characterized using GC-MS, FTIR, SEM, AFM, EDX and XRD analysis. It was mainly composed of carbohydrate (19.5%) and protein (6.0%). Box-Behnken design (BBD), using 3 level-3 variables, was employed to enhance the decolorization process by optimizing the effect of various factors. A significant enhancement from 62.50 ± 0.1 to 82.01 ± 0.8% in decolorization of wastewater was observed under optimized conditions viz. bioflocculant dosage (60 mg/L), pH (5.0) and contact time (180 min). A quadratic polynomial model was adequate beside the actual statistics at an R2 value of 0.99 for the response decolorization % and was in good agreement with the predicted value (82.01 ± 0.1%) obtained by the RSM model. The results of the present investigation demonstrated that Aloe vera mucilage can serve as a promising bioflocculant with high removal efficiency for solids, colour and dye from wastewater. To the best of our information, this is the first report on the use of Aloe vera mucilage as a natural bioflocculant for the treatment of dye-bearing wastewater.


Assuntos
Aloe , Águas Residuárias , Têxteis
10.
J Contemp Dent Pract ; 21(6): 632-635, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33025931

RESUMO

AIM: To compare the effect of three different intracanal medicaments, namely, modified triple antibiotic paste (MTAP), calcium hydroxide (Ca(OH)2), and aloe vera, on the root dentine microhardness. MATERIALS AND METHODS: A total of 50 extracted mandibular bicuspids were prepared using ProTaper Next rotary files. The roots of the bicuspids were alienated to three groups (n = 10 each) and one control group (untreated; n = 20). In three groups, the root canals were filled with MTAP, Ca(OH)2, and aloe vera medicaments. After 21 days, medicaments were removed by Endo activator. Mean Knoop hardness numbers were calculated after treatment and compared with the untreated control group. Data were evaluated using the Student's t test (paired), ANOVA (one-way) followed, and the post hoc test. RESULTS: All treated groups except the aloe vera group had shown significant reduction (p < 0.05) in microhardness of the root dentin as compared with the untreated control group. The aloe vera group showed least reduction of microhardness and was statistically insignificant (p > 0.05). CONCLUSION: Aloe vera shows promising results in terms of fewer effects on microhardness of the root dentin compared to MTAP and Ca(OH)2. CLINICAL SIGNIFICANCE: Elimination of most of the bacterial infection from the root canal and very minimum to no effect on the microhardness of the dentin in the root part are the basics of success in any endodontic treatment. Further in vivo studies are required to compare the efficacy of these intracanal medicaments.


Assuntos
Aloe , Óxido de Zinco , Antibacterianos , Hidróxido de Cálcio , Dentina , Humanos
11.
Nat Commun ; 11(1): 5162, 2020 10 14.
Artigo em Inglês | MEDLINE | ID: mdl-33056984

RESUMO

Bioactive natural C-glycosides are rare and chemical C-glycosylation faces challenges while enzymatic C-glycosylation catalyzed by C-glycosyltransferases provides an alternative way. However, only a small number of C-glycosyltransferases have been found, and most of the discovered C-glycosyltransferases prefer to glycosylate phenols with an acyl side chain. Here, a promiscuous C-glycosyltransferase, AbCGT, which is capable of C-glycosylating scaffolds lacking acyl groups, is identified from Aloe barbadensis. Based on the substrate promiscuity of AbCGT, 16 C-glycosides with inhibitory activity against sodium-dependent glucose transporters 2 are chemo-enzymatically synthesized. The C-glycoside 46a shows hypoglycemic activity in diabetic mice and is biosynthesized with a cumulative yield on the 3.95 g L‒1 scale. In addition, the key residues involved in the catalytic selectivity of AbCGT are explored. These findings suggest that AbCGT is a powerful tool in the synthesis of lead compounds for drug discovery and an example for engineering the catalytic selectivity of C-glycosyltransferases.


Assuntos
Aloe/enzimologia , Glicosídeos/biossíntese , Glicosiltransferases/metabolismo , Proteínas de Plantas/metabolismo , Inibidores do Transportador 2 de Sódio-Glicose/metabolismo , Aloxano/toxicidade , Aloe/genética , Animais , Biocatálise , Glicemia/análise , Glicemia/efeitos dos fármacos , Clonagem Molecular , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Tipo 1/sangue , Diabetes Mellitus Tipo 1/induzido quimicamente , Diabetes Mellitus Tipo 1/tratamento farmacológico , Descoberta de Drogas/métodos , Feminino , Glicosídeos/farmacologia , Glicosídeos/uso terapêutico , Glicosilação , Glicosiltransferases/genética , Glicosiltransferases/isolamento & purificação , Humanos , Masculino , Camundongos , Proteínas de Plantas/genética , Proteínas de Plantas/isolamento & purificação , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Transportador 2 de Glucose-Sódio/metabolismo , Inibidores do Transportador 2 de Sódio-Glicose/farmacologia , Inibidores do Transportador 2 de Sódio-Glicose/uso terapêutico , Especificidade por Substrato
12.
Ceska Slov Farm ; 69(3): 143-148, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32972158

RESUMO

The article presents the results of a pharmacodynamic study of a new gel containing an extract of Aloe vera and an extract of oak bark under the condition of destructive inflammatory periodontal diseases. Pharmacodynamics of the new gel was studied by the following methods: antimicrobial effect - by diffusion method in agar gel (compared product - Metrogyl denta® gel); reparative effect - on the model of linear cut wounds (compared product - Calendula ointment); anti-inflammatory activity - on the model of acute carrageenan-induced inflammation (compared product - Diclofenac natrium gel 5%). It has been established that the antimicrobial activity of the new gel against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 27853, Candida albicans NCTC 885-653, Escherichia faecalis ATCC 29212, and Staphylococcus mutans ATCC 35668 is slightly lower in comparison with Metrogyl denta® gel exhibiting a powerful antimicrobial activity. According to the reparative effect on the model of linear cut wounds, the new gel exceeded the effectiveness (by 24%, p < 0.001) of the compared drug based on the medicinal plant material - Calendula ointment. A significant anti-inflammatory activity of the new gel has been revealed under the conditions of acute carrageenan inflammation. It exceeded the Diclofenac natrium gel in the first hours of the experiment, indicating an anti-lipoxygenase activity of the new gel. The established antimicrobial, reparative and anti-inflammatory activity of a new gel containing aloe vera and oak bark extracts confirmed its potential use in the treatment of destructive inflammatory periodontal diseases.


Assuntos
Aloe/química , Doenças Periodontais , Extratos Vegetais/farmacologia , Quercus/química , Anti-Infecciosos/farmacologia , Géis , Humanos , Testes de Sensibilidade Microbiana , Doenças Periodontais/tratamento farmacológico , Casca de Planta/química
13.
Eur. j. anat ; 24(5): 343-356, sept. 2020. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-195271

RESUMO

This study assessed the effect of varying doses of aqueous extract of Aloe barbadensis on the cellular changes of hippocampal cells, oxidative and memory state of Wistar rats following monosodium glutamate-induced neurotoxicity. Eighty Wistar rats (8 weeks) were randomly as-signed into 4 groups of 20 rats; Group 1 received 3 mL/kg of distilled water. Groups 2, 3 and 4 received 3 g/kg/day of MSG. In addition, groups 3 and 4 received 100 and 200 mg/kg/day of AB ex-tract respectively. Administration was done orally for 28 days in all groups. Five rats per group were sacrificed weekly over a 4-week period. Memory was assessed using radial arm maze on the last day of administration. Following brain harvest, one cerebral hemisphere was homogenized for oxidative state assessment, while the other was fixed in 10% neutral buffered formalin and stained with H&E for hippocampal histomorphology. Data obtained were analyzed using student t-test and p value < 0.05 was considered significant. Across the 4-week period, group 2 rats showed significant increase in time spent to identify baited arms, significant reduction in density of apparentlynormal neurons and oligodendrocyte in CA 1-3 regions of hippocampus, and significant increase in reduced glutathione when compared with other groups. However, no significant differences were noted between groups 1, 3 and 4 for the above stated parameters. The study concluded that MSG caused hippocampal neuronal and oligodendrocytes degeneration and impairment of memory. These anomalies are prevented by 100 and 200 mg/kg of Aloe barbadensis


No disponible


Assuntos
Animais , Ratos , Hipocampo/anatomia & histologia , Hipocampo/efeitos dos fármacos , Glutamato de Sódio/efeitos adversos , Síndromes Neurotóxicas/veterinária , Aloe , Glutamato de Sódio/administração & dosagem , Estresse Oxidativo , Extratos Vegetais/uso terapêutico , Análise de Variância , Modelos Animais de Doenças
15.
Acta Cir Bras ; 35(5): e202000507, 2020 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-32638846

RESUMO

PURPOSE: To develop a new wound dressing composed of alginate and Aloe vera gel and cross-linked with zinc ions. METHODS: The aloe-alginate film was characterized using scanning electron microscopy (SEM), swelling profile, mechanical properties, polysaccharide content and X-ray diffraction (XRD). Thirty Wistar rats were divided in two groups a) treated with aloe-alginate film and b) control (treated with sterile gauze). Wound contraction measurements and hystological analysis were performed on 7th, 14th and 21st days after wound surgery. RESULTS: The aloe-alginate film presented adequated mechanical resistance and malleability for application as wound dressing. There was no statistical difference in wound contraction between two groups. Histological assay demonstrated that aloe-alginate film presented anti-inflammatory activity, stimulated angiogenesis on proliferative phase and a more significant increased in collagen type I fibers and decreased type III fibers which promoted a mature scar formation when compared to control. CONCLUSIONS: The aloe-alginate film showed adequate physicochemical characteristics for wound dressing applications. The in vivo assay demonstrated that aloe-alginate film enhanced the healing process of incisional skin wounds.


Assuntos
Alginatos , Aloe , Cloretos , Preparações de Plantas , Cicatrização , Compostos de Zinco , Alginatos/farmacologia , Animais , Cloretos/química , Cloretos/farmacologia , Preparações de Plantas/farmacologia , Ratos , Ratos Wistar , Cicatrização/efeitos dos fármacos , Compostos de Zinco/química , Compostos de Zinco/farmacologia
16.
Artigo em Inglês | MEDLINE | ID: mdl-32709052

RESUMO

Aloe vera has been the most useful medicinal herb in the world since ancient times due to its vast biological effects. The presence of high content of bioactive compounds make Aloe vera a promising complementary and alternative agent in disease prevention. The effectiveness of A. vera-based medicines against pesticide toxicity has never been evaluated. It was therefore envisaged to develop an A. vera-based strategy to protect the non-target animals from adverse effects of the pesticides. This article illustrates the ameliorating effect of aqueous extract (AE) of A. vera leaves against the cartap and malathion toxicity. To evaluate the protective impact of A. vera against cartap (Ctp), malathion (Mtn) and a mixture of both pesticides, the animals were divided in eight groups, each containing six rats: Group 1- C (control), Group 2- AE + C, Group 3- Ctp, Group 4- Mtn, Group 5- Ctp + Mtn, Group 6- AE + Ctp, Group 7- AE + Mtn, Group 8- AE + Ctp + Mtn. Wistar rats exposed to Ctp, Mtn and Ctp + Mtn, displayed significant change in body weight. It was observed that the WBC level increased significantly in Mtn and Ctp + Mtn challenged groups. The contents of TNF-α and IL-6 in serum increased expressively in the Ctp, Mtn and Ctp + Mtn challenged groups. Rats treated with Ctp, Mtn and Ctp + Mtn displayed significant alterations in the levels of antioxidative indices (MDA, GSH, GST, GPx, SOD and CAT). Significant alterations were recorded in the activities of AST, ALT, ACP and ALP in Ctp, Mtn and Ctp + Mtn challenged groups. The histopathological results of liver supported the biochemical data. The pre-treatment of rats with the aqueous extract of A. vera leaves significantly protected them from the toxicity of pesticides. These results suggested that A. vera extract may be used as a promising natural agent for the management of pesticide induced toxicity.


Assuntos
Aloe , Malation , Extratos Vegetais , Animais , Anti-Inflamatórios/farmacologia , Antioxidantes/metabolismo , Fígado/metabolismo , Malation/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Ratos , Ratos Wistar , Tiocarbamatos/toxicidade
17.
Water Sci Technol ; 81(3): 479-490, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32385201

RESUMO

Aloe gel (Alg), which is a natural extract from the Aloe sp. plant, was evaluated in this study for its potential use as a bioflocculant to treat urban wastewater sewage sludge. The gel was used alone and combined with water glass (WG) under controlled conditions in laboratory experiments. Alg was found effective to settle the flocculated sludge rapidly and remove distinctive unpleasant odours of the sludge as highlighted by gas chromatography-mass spectrometry (GC/MS) analysis. Furthermore, Alg was pH tolerant and had no effect in changing the pH of the wastewater. The optimum dose of Alg was 3% at which a sludge volume index (SVI) of 45.4 mL/g was obtained within 30 min settling time. To enhance the treatment performances of Alg, WG was also evaluated as an alkali agent to further reduce the chemical oxygen demand (COD) and ammonia (NH4-N) in the wastewater. At equal doses of 3% of WG and Alg each, the combined treatment outcomes showed high turbidity and NH4-N removals of 83 and 89%, respectively, but the overall COD removal was at best 25%. The settling rate of treated sludge with combined Alg/WG was very rapid giving an SVI of 25.4 mL/g within only 5 min.


Assuntos
Aloe , Esgotos , Reatores Biológicos , Odorantes , Eliminação de Resíduos Líquidos , Águas Residuárias , Água
18.
BMC Complement Med Ther ; 20(1): 146, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32404169

RESUMO

BACKGROUND: Acute radiation-induced proctitis (ARP) is the most common side effect following radiotherapy for malignant pelvic disease. This study evaluated the efficacy of Aloe vera ointment in prevention of ARP. METHODS: Forty-two patients receiving external-beam radiotherapy (RT) for pelvic malignancies were randomized to receive either Aloe vera 3% or placebo topical ointment during radiotherapy for 6 weeks. These patients were evaluated based on the severity (grade 0-4) of the following symptoms weekly: rectal bleeding, abdominal/rectal pain, diarrhea, or fecal urgency. RTOG acute toxicity criteria and psychosocial status of the patients were also recorded weekly. Lifestyle impact of the symptoms, and quantitative measurement of C-reactive protein (CRP), an indicator of systemic inflammation, were also measured. RESULTS: The results of present study demonstrated a significant preventive effect for Aloe vera in occurrence of symptom index for diarrhea (p < 0.001), rectal bleeding (p < 0.001), and fecal urgency (p = 0.001). The median lifestyle score improved significantly with Aloe vera during RT (p < 0.001). Intervention patients had a significant lower burden of systemic inflammation as the values for quantitative CRP decreased significantly over 6 weeks of follow-up (p = 0.009). CONCLUSION: This study showed that Aloe vera topical ointment was effective in prevention of symptoms of ARP in patients undergoing RT for pelvic cancers. TRIAL REGISTRATION: IRCT201606042027N6. Registration date: 2016-09-04.


Assuntos
Aloe , Neoplasias Pélvicas/radioterapia , Preparações de Plantas/uso terapêutico , Proctite/prevenção & controle , Lesões por Radiação/prevenção & controle , Idoso , Proteína C-Reativa/análise , Método Duplo-Cego , Feminino , Humanos , Irã (Geográfico) , Masculino , Pessoa de Meia-Idade , Pomadas/uso terapêutico , Estudos Prospectivos , Qualidade de Vida , Radioterapia/efeitos adversos , Inquéritos e Questionários
19.
Environ Sci Pollut Res Int ; 27(17): 20876-20888, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32249384

RESUMO

Nowadays, the sunscreen creams are composed of mostly synthetic chemicals and other organic compounds which were found to enter into the blood stream on topical application raising concerns in the scientific community. The scientific community has now shifted their attention to herbal formulations due to toxicity of these synthetic molecules. Aloe vera is a xerophitic plant having excellent anti-oxidant properties. The permeation effect and drug stability of the drug candidate can be significantly enhanced by formulating it into solid lipid nanoparticles (SLN). The main objectives of the study were to formulate and evaluate Aloe vera-loaded SLN sunscreen cream and to determine its photoprotective potential. The Aloe vera-loaded SLNs were formulated by microemulsification technique. The developed SLNs were studied for its entrapment efficiency, poly dispersity index (PDI), zeta potential, particle size, and other characterization techniques. Finally, the optimized SLNs were incorporated into the sunscreen cream and evaluated for its spreadability, viscosity, extrudability, drug content, in vitro drug release, ex vivo permeation, determination of sun protection factor (SPF), skin irritation test, and accelerated stability studies. The in vitro SPF was found out to be 16.9 ± 2.44 and the in vivo SPF observed to be approximately 14.81 ± 3.81, respectively. Stability studies were performed under accelerated conditions and no appreciable changes in the parameters were noticed. The solid lipid nanoparticles of Aloe vera were incorporated into a cream and the SPF of the resultant sunscreen cream was found to be on par with the sunscreens that were currently available in the market.


Assuntos
Aloe , Nanopartículas , Lipídeos , Tamanho da Partícula , Protetores Solares
20.
J Appl Microbiol ; 129(3): 521-531, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32281210

RESUMO

AIM: To evaluate the bacterial composition of collared peccary semen and foreskin mucosa, and to verify the sensitivity of isolates to antimicrobials used in semen conservation and to Aloe vera gel, which is an alternative external cryoprotectant. METHODS AND RESULTS: Nine foreskin mucosa and ejaculate samples from adult animals were used. Sperm characteristics and bacterial load were evaluated in fresh semen. The preputial mucosa and semen bacterial isolates were identified and tested against five concentrations of each antimicrobial (streptomycin-penicillin and gentamicin) and A. vera gel. Corynebacterium sp. and Staphylococcus sp. were isolated in greater numbers than others in both semen (64·10 and 20·51%, respectively) and the foreskin mucosa (60·60 and 24·25%, respectively), and ranged from 0·4 to 21 × 105 colony-forming units (CFU) per ml. The average load of Corynebacterium sp. was negatively correlated (P < 0·05) with the sperm membrane integrity (r = -0·73055) and curvilinear velocity (r = -0·69048). Streptomycin-penicillin and gentamicin inhibited most micro-organisms, and A. vera showed lower antimicrobial activity. CONCLUSION: Several Gram-positive bacteria are present in semen and foreskin mucosa of collared peccary, and the benefits of using primarily penicillin-streptomycin and gentamicin antimicrobials in the bacterial control of diluted semen of these animals are strongly indicated. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides insight into the reproductive microbiota of captive male-collared peccary. This work provides a theoretical basis to assist reproductive biotechnologies for ex situ conservation of the species.


Assuntos
Artiodáctilos/microbiologia , Prepúcio do Pênis/microbiologia , Microbiota , Sêmen/microbiologia , Espermatozoides/fisiologia , Aloe , Animais , Antibacterianos/farmacologia , Artiodáctilos/fisiologia , Bactérias Aeróbias/classificação , Bactérias Aeróbias/efeitos dos fármacos , Bactérias Aeróbias/isolamento & purificação , Crioprotetores/farmacologia , Masculino , Membrana Mucosa/microbiologia , Espermatozoides/citologia
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