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1.
Sci Total Environ ; 802: 149779, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34525680

RESUMO

The importance of evaluating how natural organic matter influences the mobility of arsenic species in an ecosystem is an environmental concern. This work aimed to evaluate the interaction between humic substances (HS) and four arsenic species of high toxicity [As(III), As(V), MMA(V), and DMA(V)] (HS-As) under the influence of HS concentration and pH. Next, the complexing capacity (CC) of HS by As(III) was determined in function of pH, ionic strength and co-existing ions. The free arsenic (Asfree) was determined after a tangential flow ultrafiltration procedure by hydride generation atomic fluorescence spectrometry. The better HS-As interactions for As(III) and As(V) at pH 10.5 reached 52% and 61%, respectively. The increase in pH and ionic strength, as well as co-existing ions increased the CC, which reached 1.57 mg of As(III) g-1 HS. We proposed a HS-As interaction model based on the inner and outer binding sites of HS from these results. The inner sites were occupied through hydrogen bonds, Pearson acid-base, hydrophobic, and van der Waals interactions for trivalent arsenic species, while the interactions through the outer sites for pentavalent arsenic species were mostly by hydrogen bonds and electrostatic forces. According to ecotoxicological studies against Artemia salina, the presence of HS decreased the toxicity of As(III) and As(V) as the lethal concentration increased from 5.81 to 8.82 mg L-1 and from 8.82 to 13.37 mg L-1, respectively. From the results through the proposed model, it was possible to successfully understand the interaction dynamic between soil HS and As(III), As(V), MMA(V) and DMA(V) under simulated environmental conditions.


Assuntos
Arsênio , Arsenicais , Ecossistema , Substâncias Húmicas , Espectrometria de Fluorescência
2.
Sci Total Environ ; 802: 149776, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34525751

RESUMO

Macroalgae can cycle arsenic (As) in the environment. In this study, the role of iron (Fe) plaque manipulation at active sites in the As biotransformation mechanism was investigated. The strain of marine macroalgal species, Pyrophia yezoensis, was inoculated in association with arsenate (As(V)) (1.0 µmol L-1) and phosphate (10 µmol L-1) in the medium for 7 days under laboratory-controlled conditions. The Fe plaque was removed by washing the Ti(III)-citrate-EDTA solution before inoculation. The limitation of Fe plaque did not significantly (p > 0.05) affect the chlorophyll fluorescence due to cellular regeneration, which was initiated immediately after washing. However, the speciation and uptake rate of As(V) increased significantly and reduced the inhibitory effect of P on the intracellular uptake of As(V) by P. yezoensis. In the culture medium without Fe plaque, approximately 66% of As(V) was removed with Vmax = 0.32 and Km = 1.92. In the absence of Fe plaque, methylated As species, such as dimethylarsinate (DMAA(V)), was recorded 0.28 µmol L-1, while in the presence of Fe plaque, the value was 0.16 µmol L-1. Inorganic trivalent As (As(III)) was absent in the washed samples; however, 0.53 µmol L-1 concentration of As(III) was still found in the presence of Fe plaque on day 7 of incubation. The results indicated that the absence of Fe plaque promoted higher intracellular uptake of As species, reduced the inhibitory effect of P, mitigated the co-precipitation bond between AsFe plaque and enhanced the detoxification process by DMAA excretion from the cell.


Assuntos
Arsênio , Arsenicais , Alga Marinha , Biotransformação , Ácido Cacodílico
3.
Talanta ; 236: 122813, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34635209

RESUMO

Bacillus thuringiensis (Bt) is used as a bioinsecticide since it effectively kills insect larvae. Bt is also genetically similar to Bacillus cereus (Bc), a well recognized foodborne human pathogen; they are both members of the Bacillus cereus group (BC group). Although approved Bt bioinsecticide products have been confirmed to be non-pathogenic to humans, close monitoring of Bt during dissemination is important for cost considerations and to limit impact on biodiversity towards nontarget organisms. As such, developing rapid, sensitive, and specific tools for quantitative detection of Bt spores during and following spray operations is highly desirable. The goals of this study were to investigate commercially available detection reagents for sensitivity and selectivity in detecting Bt spores, and then functionalize a surface of (001) GaAs used in photonic biosensing. To achieve these goals, we (1) screened commercial antibodies for their capacity to bind recombinant proteins from Bt spores, (2) screened antibodies and aptamers for their sensitivity and selectivity against Bt spores, and (3) tested the efficiency of selected antibodies and aptamers in capturing Bt spores on the surface of functionalized GaAs biochips. Seven genes encoding Bt spore proteins were cloned and expressed in Escherichia coli. The binding of each purified spore antigen was tested by commercially available polyclonal and monoclonal antibodies claimed to exclusively target spores. Of the seven targets, Bacillus collagen-like protein A, was the most abundant protein on Bt spores and demonstrated the strongest binding affinity to all test antibodies. The commercial antibodies (Abs) were also tested for specificity to BC Group versus non-BC Group spores. Three of six commercial antibodies showed selectivity to Bt spores, with recombinant Abs providing the most robust lower range of detection (102 to 6 × 103 spores/mL). The sensitivity and selectivity of three published DNA aptamer sequences demonstrated a wide range of detection sensitivity for Bt spores. Two of the three test aptamers also showed reasonable selectivity towards Bt spores while the third demonstrated reactivity to non-BC Group B. megaterium and B. subtilis. Of the reagents tested, a thiolated aptamer and llama recombinant Ab showed highest Bt spore capture efficiency as measured by spore coverage of the GaAs surface. These results confirm that the selected aptamer and llama rAb can be considered strong candidates for the development of GaAs-based biosensing devices.


Assuntos
Bacillus anthracis , Bacillus thuringiensis , Técnicas Biossensoriais , Arsenicais , Bacillus thuringiensis/genética , Gálio , Humanos , Esporos Bacterianos
4.
Chemosphere ; 286(Pt 1): 131638, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34303908

RESUMO

Soil samples were collected from a representative arsenic (As) contaminated region under phytoremediation of hyperaccumulation plants. Relative abundance and diversity of microbial communities in the soil samples were characterized via 16S rRNA genes sequencing. At the phylum level, Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Gemmatimonadetes and Firmicutes shows the highest abundance, accounting for more than 90 % of the classified sequences in the soil samples. Physicochemical parameters including pH, total organic carbon (TOC), cation exchange capacity (CEC), and electrical conductivity (EC), and heavy metal concentrations including total and bioaccessible contents in the soil samples were determined to investigate potential relationships between the microbial communities and the environmental factors. Principal component analysis (PCA) based on the operational taxonomic units (OTUs) matrix revealed distinct separation among the samples. The soil pH was confirmed as the dominant force to discriminate the soil samples with similar land use type and heavy metal contamination. There was little relevance between the total concentrations of heavy metals and the microbial communities. However, the bioaccessible concentrations of heavy metals were associated with the physicochemical parameters and relative abundances of bacterial genera according to correlation analyses. Although the soil samples were considerably contaminated by As, the abundances of bacterial phyla linked with As were lower than 1.0 % in most of the soil samples. The results indicated that the abundances of microbial communities in the soils were the consequence of concerted effects from all the environmental factors.


Assuntos
Metais Pesados , Microbiota , Poluentes do Solo , Arsenicais , Metais Pesados/análise , RNA Ribossômico 16S/genética , Solo , Microbiologia do Solo , Poluentes do Solo/análise , Sulfetos
5.
Ecotoxicol Environ Saf ; 227: 112934, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34755630

RESUMO

Long-term consumption of arsenic-contaminated rice has become a public health issue that urgently needs to be addressed. In this study, mice were exposed to arsenic in rice (low dose, 0.91 mg/kg; medium dose, 9.1 mg/kg) for 30 days and 60 days, respectively, and the effects on pathological structures of spleen and skin, as well as the structure of the fecal microbiome were examined. The findings revealed dose/time cumulative effects on pathological changes, with even a low dose exposure for 30 days causing destruction of splenic follicular structure and thickening of dermal keratinized and epidermal layers. The Firmicutes/Bacteroidetes ratio in the community and the positive/negative ratio in network links were higher in arsenic groups, suggesting that arsenic resulted in a less healthy and unstable microbiome for the host. Thus lifetime consumption of arsenic in rice may have potential health effects on humans and must be carefully assessed to safeguard human health. Furthermore, in arsenic groups, arsenic-resistant bacteria or arsenic hazards remediation bacteria changed to be the dominant bacteria and acted as the core bacteria in the network modules. Some microbial arsenic transforming genes (arsC, arsR, arsA, ACR3, and aoxB) differed, indicating that the gut microbiome changed to withstand arsenic stress. Furthermore, Faecalibaculum, Lachnospiraceae_NK4A136_group, Angelakisella, Ruminiclostridium, and Desulfovibrionaceae are positively associated with arsenic dosage and may be useful in the early detection of arsenicals.


Assuntos
Arsênio , Arsenicais , Microbioma Gastrointestinal , Microbiota , Oryza , Animais , Arsênio/toxicidade , Camundongos
6.
Shokuhin Eiseigaku Zasshi ; 62(5): 139-147, 2021.
Artigo em Japonês | MEDLINE | ID: mdl-34732639

RESUMO

We have developed a quantitative determination method of the concentration of inorganic arsenic in pet foods using a liquid chromatograph-inductively coupled plasma-mass spectrometer (LC-ICP-MS). After adding 2 w/v% TMAH solution to a sample, inorganic arsenic was extracted by heating and the extract was collected by water. The pH of the solution was adjusted, and injected into a LC-ICP-MS to determine the concentration of inorganic arsenic. LC separation was carried out on an ODS column with 10 mmol/L sodium 1-butanesulfonate, 4 mmol/L malonic acid, 4 mmol/L TMAH and 0.05% methanol solution as a mobile phase. A collaborative study was conducted by nine laboratories using dry and wet-type pet foods, formed jerky, dried jerky and biscuit. Dry-type pet food and dried jerky was added with 2 mg/kg of As (III). Wet-type pet food was added with 0.5 mg/kg of As (III). Formed jerky was added with 1 mg/kg of As (III). Biscuit was added with 0.2 mg/kg of As (III). The mean recoveries, repeatabilities and reproducibilities in the form of relative standard deviation (RSDr and RSDR), and HorRat, were 95.4% to 98.3%, less than 2.9%, less than 9.1%, and 0.22 to 0.51, respectively.


Assuntos
Arsênio , Arsenicais , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , Alimentos Marinhos/análise
7.
Anal Chem ; 93(45): 15115-15123, 2021 11 16.
Artigo em Inglês | MEDLINE | ID: mdl-34714618

RESUMO

Designing new catalysts with high activity and stability is crucial for the effective analysis of environmental pollutants under mild conditions. Here, we developed a superior catalyst of Pt single atoms anchored on MoS2 (Pt1/MoS2) to catalyze the determination of As(III). A detection sensitivity of 3.31 µA ppb-1 was obtained in acetate buffer solution at pH 6.0, which is the highest compared with those obtained by other Pt-based nanomaterials currently reported. Pt1/MoS2 exhibited excellent electrochemical stability during the detection process of As(III), even in the coexistence of Cu(II), Pb(II), and Hg(II). X-ray absorption fine structure spectroscopy and theoretical calculations revealed that Pt single atoms were stably fixed by four S atoms and activated the adjacent S atoms. Then, Pt and S atoms synergistically interacted with O and As atoms, respectively, and transferred some electrons to H3AsO3, which change the rate-determining step of H3AsO3 reduction and reduce reaction energy barriers, thereby promoting rapid and efficient accumulation for As(0). Compared with Pt nanoparticles, the weaker interaction between arsenic species and Pt1/MoS2 enabled the effortless regeneration and cyclic utilization of active centers, which is more favorable for the oxidation of As(0). This work provides inspiration for developing highly efficient sensing platforms from the perspective of atomic-level catalysis and affords references to explore the detection mechanism of such contaminants.


Assuntos
Arsenicais , Nanoestruturas , Arsenicais/química , Catálise , Molibdênio , Oxirredução , Platina
8.
Aquat Toxicol ; 241: 105993, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34688139

RESUMO

Recent studies have found primary degradation products of phenylarsenic chemical warfare agents (CWAs) accumulating in fish tissues, while the potential effects of these dumped phenylarsenic CWAs, such as Clark I and II, in the Baltic Sea biota are poorly understood. In this study, the metabolism and cytotoxicity of diphenylarsinic acid (DPA), a primary degradation product of phenylarsenic CWA, was studied by incubating rainbow trout cell line RTL-W1 cells in media with 100 mg/L DPA. Previously undescribed metabolites were identified by ultra-high performance liquid chromatography-high resolution mass spectrometry (UPHLCHRMS). Moreover, the cytotoxicity of diphenylarsine glutathione conjugate (DPA-SG), the major metabolite of DPA, was studied. Cytotoxicity of the compounds was evaluated using the Neutral Red retention test (NRR), showing an IC50 value of 278 mg/L for DPA and 1.30 mg/L for DPA-SG, indicating that the glutathione (GSH) conjugate of DPA is more than two orders of magnitude toxic than DPA itself, suggesting that toxic properties of DPA are increased after conjugation with intracellular GSH leading enhanced toxicity after uptake. Results gained in this study give more detailed information for elucidating biological effects of dumped chemical munitions in marine environment. Moreover, the results help in assessing the environmental and health risks posed by marine munition continued presence and deterioration in the sea bottom.


Assuntos
Substâncias para a Guerra Química , Oncorhynchus mykiss , Poluentes Químicos da Água , Animais , Arsenicais , Linhagem Celular , Substâncias para a Guerra Química/análise , Substâncias para a Guerra Química/toxicidade , Fígado/química , Poluentes Químicos da Água/toxicidade
9.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(5): 1504-1509, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34627431

RESUMO

OBJECTIVE: To investigate the effect of arsenic disulfide (AS2S2) combined with itraconazole on the proliferation, apoptosis and hedgehog pathway of diffuse large B-cell lymphoma (DLBCL) cells. METHODS: The human DLBCL cell OCI-LY3 was treated with different concentrations of AS2S2 and itraconazole. Cell proliferation inhibition was detected by CCK-8, cell apoptosis rate was determined by flow cytometry. The expression levels of BCL-2, BAX, SMO and GLi1 were detected by Western blot. RESULTS: The DLBCL cell viability was decreased significantly at 24, 48 or 72 h as cultured with itraconazole. Along with the increasing of itraconazole concentration, the DLBCL cell viability was significantly reduced as compared with that in control group, and the results showed statistically significant(r=-0.690,r=-0.639, r=-0.833, r=-0.808, r=-0.578). The inhibitory and apoptosis rates of the cells were significantly increased as compared with those of the single drug-treated group after treated by the combination of itraconazole and AS2S2(P<0.05). The protein levels of SMO and Glil were significantly down-regulated after treated by arsenic disulfide and itraconazole alone(P<0.01). The protein expression levels of SMO and Glil was down-regulated in the combined-treatment group(P<0.01). CONCLUSION: Itraconazole can inhibit proliferation of DLBCL cells in a concentration-and time-dependent manner. In addition, the combination of AS2S2 and itraconazole show a synergistic effects, which may be related with the down-regulated protein expression of SMO and Glil of Hedgehog signaling pathway.


Assuntos
Proteínas Hedgehog , Linfoma Difuso de Grandes Células B , Apoptose , Arsenicais , Humanos , Itraconazol/farmacologia , Linfoma Difuso de Grandes Células B/tratamento farmacológico , Sulfetos
10.
In Vivo ; 35(6): 3157-3163, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34697146

RESUMO

BACKGROUND/AIM: The clinical use of arsenic trioxide (As2O3) is hampered due to its cardiotoxicity. Therefore, it is critical to prevent As2O3-induced loss of endothelial integrity. The purpose of this study was to examine As2O3-induced endothelial dysfunction and evaluate the efficacy of crocetin on reversing As2O3-induced cardiotoxicity. MATERIALS AND METHODS: Cultured human umbilical vein endothelial cells (HUVECs) were used to examine As2O3-induced oxidative stress, apoptosis, production of reactive oxygen species (ROS) and DNA adducts. In addition, the impact of crocetin on As2O3-induced cardiotoxicity was evaluated. RESULTS: As2O3 decreased the viability of HUVEC cells and led to apoptosis. Additionally, As2O3 elevated NADPH oxidase activity, and the levels of intracellular ROS. Furthermore, the formamidopyrimidine DNA-glycosylase- and endonuclease III-digestible adducts were induced by As2O3 Crocetin treatment reversed the As2O3-induced reduction in cell viability, the induction of apoptosis, the activation of NADPH oxidase activity, ROS levels and DNA adducts. CONCLUSION: Crocetin protects from As2O3-induced cardio-toxicity.


Assuntos
Arsenicais , Apoptose , Trióxido de Arsênio , Carotenoides , Células Endoteliais da Veia Umbilical Humana , Humanos , Estresse Oxidativo , Óxidos/toxicidade , Espécies Reativas de Oxigênio , Vitamina A/análogos & derivados
11.
Aquat Toxicol ; 240: 105991, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34673466

RESUMO

Arsenobetaine (AsB) is the major form of arsenic in marine fish; however, its biodynamics within the fish tissues is not well understood. This study simulated the biodynamics and biotransportation (absorption, distribution, and elimination) of dietary AsB and arsenate [As(V)] in the marine grouper Epinephelus fuscoguttatus, by constructing a physiologically based pharmacokinetic (PBPK) model. The transfer rates between different compartments (gill, intestine, liver, heart, kidney, and muscle) and blood were modeled during exposure (14 d) and depuration (20 d). The model showed that AsB had a weak ability to cross the intestinal membranes and circulated slowly in the blood. The newly AsB absorbed from the blood did not enter the hepatointestinal circulation for elimination, but was effectively distributed in liver. Thereafter, it was slowly absorbed and finally stored in the muscle, the most important organ for AsB deposition, at a constant rate of 63.5 d-1. In contrast, As(V) displayed a dynamic behavior, including rapid crossing through the intestinal membranes, quick circulation in the blood and transportation to other tissues, and elimination. Biodynamics coupled with biotransformation illustrated, for the first time, the unique strategies of dietary AsB that passed slowly through the fish intestine with the highest deposition rate in the muscle, thereby contributing to the high AsB bioaccumulation in the muscle tissue of marine fish. CAPSULE: AsB displayed a weaker ability to cross the intestine membranes, slowly absorbed and finally stored in muscle, whereas As(V) displayed rapid crossing the intestine membranes, quick transportation, and elimination.


Assuntos
Arsênio , Arsenicais , Bass , Poluentes Químicos da Água , Animais , Arseniatos , Poluentes Químicos da Água/toxicidade
12.
Chem Biol Interact ; 350: 109654, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34634268

RESUMO

Since their use during the First World War, Blister agents have posed a major threat to the individuals and have caused around two million casualties. Major incidents occurred not only due to their use as chemical warfare agents but also because of occupational hazards. Therefore, a clear understanding of these agents and their mode of action is essential to develop effective decontamination and therapeutic strategies. The blister agents have been categorised on the basis of their chemistry and the biological interactions that entail post contamination. These compounds have been known to majorly cause blisters/bullae along with alkylation of the contaminated DNA. However, due to the high toxicity and restricted use, very little research has been conducted and a lot remains to be clearly understood about these compounds. Various decontamination solutions and detection technologies have been developed, which have proven to be effective for their timely mitigation. But a major hurdle seems to be the lack of proper understanding of the toxicological mechanism of action of these compounds. Current review is about the detailed and updated information on physical, chemical and biological aspects of various blister agents. It also illustrates the mechanism of their action, toxicological effects, detection technologies and possible decontamination strategies.


Assuntos
Vesícula/induzido quimicamente , Substâncias para a Guerra Química/química , Substâncias para a Guerra Química/toxicidade , Descontaminação/métodos , Alquilantes/química , Alquilantes/toxicidade , Arsenicais/efeitos adversos , Arsenicais/química , Vesícula/terapia , Substâncias para a Guerra Química/classificação , Olho/efeitos dos fármacos , Humanos , Pulmão/efeitos dos fármacos , Modelos Biológicos , Compostos de Mostarda/química , Compostos de Mostarda/toxicidade , Oximas/química , Oximas/toxicidade , Fosgênio/química , Fosgênio/toxicidade , Pele/efeitos dos fármacos
13.
Molecules ; 26(20)2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34684804

RESUMO

This paper presents a new method for the simultaneous speciation analysis of arsenic (As(III)-arsenite, As(V)-arsenate, DMA-dimethylarsinic acid, MMA-methylarsonic acid, and AsB-arsenobetaine) and selenium (Se(IV)-selenite, Se(VI)-selenate, Se-Methionine, and Se-Cystine), which was applied to a variety of seafood and onion samples. The determination of the forms of arsenic and selenium was undertaken using the High-Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP-MS) analytical technique. The separation of both organic and inorganic forms of arsenic and selenium was performed using two analytical columns: an anion exchange column, Dionex IonPac AS22, containing an alkanol quaternary ammonium ion, and a double bed cation-anion exchange guard column, Dionex Ion Pac CG5A, containing, as a first layer, fully sulfonated latex for cation exchange and a fully aminated layer for anion exchange as the second layer. The ammonium nitrate, at pH = 9.0, was used as a mobile phase. The method presented here allowed us to separate the As and Se species within 10 min with a suitable resolution. The applicability was presented with different sample matrix types: seafood and onion.


Assuntos
Arsênio/análise , Contaminação de Alimentos/análise , Cebolas/química , Cebolas/toxicidade , Alimentos Marinhos/análise , Alimentos Marinhos/toxicidade , Selênio/análise , Animais , Intoxicação por Arsênico , Arsenicais/análise , Cromatografia Líquida de Alta Pressão , Cromatografia por Troca Iônica , Humanos , Espectrometria de Massas , Compostos Organosselênicos/análise , Compostos Organosselênicos/toxicidade , Compostos de Selênio/análise , Compostos de Selênio/toxicidade
14.
Arch Biochem Biophys ; 712: 109027, 2021 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-34520732

RESUMO

The dithiol reagents phenylarsine oxide (PAO) and dibromobimane (DBrB) have opposite effects on the F1FO-ATPase activity. PAO 20% increases ATP hydrolysis at 50 µM when the enzyme activity is activated by the natural cofactor Mg2+ and at 150 µM when it is activated by Ca2+. The PAO-driven F1FO-ATPase activation is reverted to the basal activity by 50 µM dithiothreitol (DTE). Conversely, 300 µM DBrB decreases the F1FO-ATPase activity by 25% when activated by Mg2+ and by 50% when activated by Ca2+. In both cases, the F1FO-ATPase inhibition by DBrB is insensitive to DTE. The mitochondrial permeability transition pore (mPTP) formation, related to the Ca2+-dependent F1FO-ATPase activity, is stimulated by PAO and desensitized by DBrB. Since PAO and DBrB apparently form adducts with different cysteine couples, the results highlight the crucial role of cross-linking of vicinal dithiols on the F1FO-ATPase, with (ir)reversible redox states, in the mPTP modulation.


Assuntos
Cisteína/química , Poro de Transição de Permeabilidade Mitocondrial/metabolismo , ATPases Translocadoras de Prótons/metabolismo , Animais , Arsenicais/farmacologia , Compostos Bicíclicos com Pontes/farmacologia , Cálcio/metabolismo , Ditioeritritol/farmacologia , Ativadores de Enzimas/farmacologia , Inibidores Enzimáticos/farmacologia , Magnésio/metabolismo , Mitocôndrias/efeitos dos fármacos , Oxirredução/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , ATPases Translocadoras de Prótons/antagonistas & inibidores , ATPases Translocadoras de Prótons/química , Suínos
15.
J Environ Sci (China) ; 108: 1-7, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34465424

RESUMO

The distribution and chemical speciation of arsenic (As) in different sized atmospheric particulate matters (PMs), including total suspended particles (TSP), PM10, and PM2.5, collected from Baoding, China were analyzed. The average total mass concentrations of As in TSP, PM10, and PM2.5 were 31.5, 35.3, and 54.1 µg/g, respectively, with an order of PM2.5 >PM 10 > TSP, revealing that As is prone to accumulate on fine particles. Due to the divergent toxicities of different As species, speciation analysis of As in PMs is further conducted. Most of previous studies mainly focused on inorganic arsenite (iAsIII), inorganic arsenate (iAsV), monomethylarsonate (MMA), and dimethylarsinate (DMA) in PMs, while the identification and sensitive quantification of trimethylarsine oxide (TMAO) were rarely reported. In this study, a high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry system was optimized for As speciation including TMAO in PMs. An anion exchange column was used to separate MMA, DMA and iAsV, while a cation exchange column to separate TMAO and iAsIII. Results showed that iAsV was the dominate component in all the samples, corresponding to a portion of 79.2% ± 9.3% of the total extractable species, while iAsIII, TMAO and DMA made up the remaining 21%. Our study demonstrated that iAsIII accounted for about 14.4% ± 11.4% of the total extracted species, with an average concentration of 1.7 ± 1.6 ng/m3. It is worth noting that TMAO was widely present in the samples (84 out of 97 samples), which supported the assumption that TMAO was ubiquitous in atmospheric particles.


Assuntos
Arsênio , Arsenicais , Arsênio/análise , Arsenicais/análise , Ácido Cacodílico , China , Cromatografia Líquida de Alta Pressão , Material Particulado/análise
16.
J Hazard Mater ; 416: 126149, 2021 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492933

RESUMO

As the typical hazardous arsenic pollutants, copper smelting flue dust (CSFD) and arsenic sulfide residue (ASR) are produced extensively during copper smelting process, which pose significant pressure on environmental protection and green development of the copper industry. This work proposed an economic, efficient, and applicable approach to treat waste with waste, in which the simultaneous removal and recovery of As from CSFD and ASR were realized by a roasting process, with adding sulfuric acid, at a relatively low temperature (300-350 â„ƒ). The thermodynamic analysis and experiments confirmed that the main phases of As2S3 and S0 in the ASR were used as a reductant for reducing As(Ⅴ) in the CSFD, and the introduction of sulfuric acid favorably enhanced the thermodynamic driving force and greatly lowered the reaction temperature. The results indicated that removal and behavior of As were highly dependent on the mass ratio of ASR to CSFD, roasting temperature, and H2SO4 dosage. By regulating the parameters, the species As2S3, As2O5, and arsenate were all converted to volatile As2O3, which could be captured and deposited in cold water. In the optimized co-treatment, a satisfied As removal efficiency of 96.12% was achieved, while getting the 97.03% pure As2O3.


Assuntos
Arsênio , Arsenicais , Cobre , Poeira , Sulfetos
17.
Int J Hyg Environ Health ; 237: 113834, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34488179

RESUMO

Rice consumption is an important source of arsenic exposure. Little has known about the impact of rice consumption on arsenic metabolism, which is related to insulin resistance. In this study, we examined the associations between rice consumption and arsenic metabolism, and between arsenic metabolism and insulin resistance in non-diabetic U.S adults who participated in the National Health and Nutrition Examination Survey (NHANES) 2003-2016. Rice consumer was defined as ≥0.25 cups of cooked rice/day. HOMA2-IR was calculated using HOMA2 Calculator software based on participant's fasting glucose and insulin values. Urinary arsenic concentrations below limits of detection were imputed first, and then arsenic metabolism (the proportions of inorganic arsenic (iAs), monomethylarsonate (MMA), and dimethylarsinate (DMA) to their sum) were calculated (expressed as iAs%, MMA%, and DMA%). Using the leave-one-out approach, rice consumers compared with non-consumers had a 1.71% (95% CI: 1.12%, 2.29%) higher DMA% and lower MMA% when iAs% fixed; a 1.55% (95% CI: 0.45%, 2.66%) higher DMA% and lower iAs% when MMA% fixed; and a 1.62% (95% CI: 0.95%, 2.28%) higher iAs% and lower MMA% when DMA% fixed, in multivariable adjustment models. With every 10% decrease in MMA%, the geometric mean ratio of HOMA2-IR was 1.06 (95% CI: 1.03,1.08) and 1.05 (95% CI: 1.02, 1.09) when DMA% and iAs% was fixed, respectively; however, the associations were attenuated after adjusting for body mass index. In stratified analysis, we found that lower MMA% was associated with higher HOMA2-IR in participants with obesity: a 10% increase in iAs% with a 10% decrease in MMA% was associated with higher HOMA2-IR with the geometric mean ratio of 1.05 (95% CI: 1.01, 1.09). Our findings suggest that rice consumption may contribute to lower MMA% that was further associated with higher insulin resistance, especially in individuals with obesity. Future prospective studies are needed to confirm our results in different populations.


Assuntos
Arsênio , Arsenicais , Diabetes Mellitus , Resistência à Insulina , Oryza , Adulto , Arsênio/análise , Exposição Ambiental/análise , Humanos , Inquéritos Nutricionais
18.
Artigo em Inglês | MEDLINE | ID: mdl-34574656

RESUMO

Skeletal muscle mass reduction has been implicated in insulin resistance (IR) that promotes cardiometabolic diseases. We have previously reported that arsenic exposure increases IR concomitantly with the reduction of skeletal muscle mass among individuals exposed to arsenic. The arsenic methylation capacity is linked to the susceptibility to some arsenic exposure-related diseases. However, it remains unknown whether the arsenic methylation capacity affects the arsenic-induced reduction of muscle mass and elevation of IR. Therefore, this study examined the associations between the arsenic methylation status and skeletal muscle mass measures with regard to IR by recruiting 437 participants from low- and high-arsenic exposure areas in Bangladesh. The subjects' skeletal muscle mass was estimated by their lean body mass (LBM) and serum creatinine levels. Subjects' drinking water arsenic concentrations were positively associated with total urinary arsenic concentrations and the percentages of MMA, as well as inversely associated with the percentages of DMA and the secondary methylation index (SMI). Subjects' LBM and serum creatinine levels were positively associated with the percentage of DMA and SMI, as well as inversely associated with the percentage of MMA. HOMA-IR showed an inverse association with SMI, with a confounding effect of sex. Our results suggest that reduced secondary methylation capacity is involved in the arsenic-induced skeletal muscle loss that may be implicated in arsenic-induced IR and cardiometabolic diseases.


Assuntos
Arsênio , Arsenicais , Arsênio/análise , Arsênio/toxicidade , Exposição Ambiental/estatística & dados numéricos , Humanos , Metilação , Músculo Esquelético
19.
J Control Release ; 338: 190-200, 2021 10 10.
Artigo em Inglês | MEDLINE | ID: mdl-34428479

RESUMO

Discovery of BCR-ABL1 tyrosine kinase inhibitors (TKIs) has revolutionized the therapy of chronic myeloid leukemia (CML), a malignant myeloproliferative disease characterized by abnormal activation of BCR-ABL fusion oncoprotein with protein tyrosine kinase (PTK) activity. However, the long-term treatment outcomes with TKIs are strongly limited by multiple drug resistances, resulting in relapse albeit with initial high response rate. Here, we reported a realgar (As4S4) nanocrystal-based delivery system to reverse drug resistance for synergistic CML therapy. While As4S4 is extremely insoluble in water, bovine serum albumin (BSA) was rationally screened to effectively stabilize As4S4 nanocrystal with uniformed size of ~40 nm. Imatinib (IMA), a representative TKIs, can be readily loaded into the hydrophobic domain of BSA to develop As4S4/IMA co-delivery system. Mechanistically, IMA inhibits PTK activity, while As4S4 degrades BCR-ABL1, which co-contribute to tumor suppression via complementary pathways for synergistic effect. Moreover, the nanosystem was modified with folic acid (FA) to enable tumor targetability, which has been demonstrated both in vitro and in vivo, resulting in robust tumor growth inhibition and significantly prolonged mice survival without any noticeable adverse effects. This work designed a synergistic nanoplatform for targeted CML therapy, provided a strategy to address the key limitation of As4S4 for biomedical applications, and highlighted the advantages of the combination between traditional Chinese and western medicine for diseases treatment.


Assuntos
Antineoplásicos , Leucemia Mielogênica Crônica BCR-ABL Positiva , Nanopartículas , Animais , Antineoplásicos/uso terapêutico , Apoptose , Arsenicais , Resistencia a Medicamentos Antineoplásicos , Proteínas de Fusão bcr-abl , Mesilato de Imatinib/farmacologia , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Camundongos , Inibidores de Proteínas Quinases/uso terapêutico , Sulfetos
20.
Zhonghua Yi Xue Za Zhi ; 101(30): 2387-2391, 2021 Aug 10.
Artigo em Chinês | MEDLINE | ID: mdl-34404132

RESUMO

Objective: To analyze the effect of triple-induction regimen including all-trans retinoic acid(ATRA), arsenic trioxide(ATO) plus anthracyclines and double-induction regimen including ATRA and ATO for adults with non-high-risk acute promyelocytic leukemia(APL). Methods: The clinical data of adult patients with non-high-risk APL who were first diagnosed and admitted to the Henan Provincial People's Hospital from January 2009 to December 2019 were retrospectively analyzed. All patients were divided into triple-induction group and double-induction group according to the treatment. The general data of patients, blood routine, coagulation function changes and blood transfusions during the induction period were collected, and the complete remission rate, early mortality and prognosis of two groups were analyzed. Results: A total of 164 patients were enrolled, including 86 males and 78 females, and the M(Q1,Q3) of their age was 41(18, 70) years. Among them, 75 were in triple-induction group and 89 in double-induction group. The white blood cell(WBC) counts of triple-induction group on day 7th and 14th after induction were (9.49±6.10)×109/L and (5.43±3.97)×109/L, while those in double-induction group were (15.17±17.06)×109/L and (13.37±12.59)×109/L, the differences were statistically significant (both P<0.05). In addition, the peak of WBC in the triple-induction group was lower than that in the double-induction group [13.8(6.3,89.7)×109/L vs 19.2(3.8,112.8)×109/L, P=0.019]. On day 7th after induction, the platelet(PLT) counts in the triple-induction group was lower than that in the double-induction group [27(11,147)×109/L vs 45(8, 183)×109/L, P=0.014]. However, the difference was not statistically significant in PLT counts between the two groups on day 14th, 21st and 28th, or in PLT transfusions during induction (all P>0.05). After treatment, it was observed only in a few patients of two groups that the prothrombin time(PT) elongation ≥3 s and/or activated partial thromboplastin time(APTT) elongation ≥10 s, and the difference was not statistically significant (all P>0.05). The incidence of induced differentiation syndrome in the triple-induction group was lower than that in the double-induction group (2.7% vs 12.4%, P=0.022) The early mortality rate was lower than that in the double-induction group (1.3% vs 5.6%), but the difference was not statistically significant (P>0.05). There were no statistically significant differences in the early complete remission rate, genetic remission rate, molecular remission rate, relapse rate, overall survival (OS) rate and disease-free survival (DFS) rate between the two groups. Conclusion: For adults with non-high-risk APL, the triple-induction therapy can reduce the counts and peaks of WBC, and reduce the incidence of induced differentiation syndrome.


Assuntos
Arsenicais , Leucemia Promielocítica Aguda , Adulto , Antraciclinas/uso terapêutico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Trióxido de Arsênio/uso terapêutico , Arsenicais/uso terapêutico , Feminino , Humanos , Leucemia Promielocítica Aguda/tratamento farmacológico , Masculino , Óxidos/uso terapêutico , Indução de Remissão , Estudos Retrospectivos , Resultado do Tratamento , Tretinoína/uso terapêutico
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