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1.
Zool Res ; 44(1): 78-89, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36349358

RESUMO

Largemouth bass ( Micropterus salmoides) is an economically important fish species in North America, Europe, and China. Various genetic improvement programs and domestication processes have modified its genome sequence through selective pressure, leaving nucleotide signals that can be detected at the genomic level. In this study, we sequenced 149 largemouth bass fish, including protospecies (imported from the US) and improved breeds (four domestic breeding populations from China). We detected genomic regions harboring certain genes associated with improved traits, which may be useful molecular markers for practical domestication, breeding, and selection. Subsequent analyses of genetic diversity and population structure revealed that the improved breeds have undergone more rigorous genetic changes. Through selective signal analysis, we identified hundreds of putative selective sweep regions in each largemouth bass line. Interestingly, we predicted 103 putative candidate genes potentially subjected to selection, including several associated with growth (p sst1 and grb10), early development ( klf9, sp4, and sp8), and immune traits ( pkn2, sept2, bcl6, and ripk2). These candidate genes represent potential genomic landmarks that could be used to improve important traits of biological and commercial interest. In summary, this study provides a genome-wide map of genetic variations and selection footprints in largemouth bass, which may benefit genetic studies and accelerate genetic improvement of this economically important fish.


Assuntos
Bass , Animais , Bass/genética , Análise de Sequência de DNA/veterinária , Genoma , América do Norte , China
2.
J Sci Food Agric ; 103(1): 152-163, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35848059

RESUMO

BACKGROUND: The Maillard reaction is a promising and safe method for obtaining chitooligosaccharide conjugates with proteins or peptides as food preservatives. This study aims to investigate the moisture state, physicochemical properties, and shelf-life of sea bass fillets treated with ε-polylysine (ε-PL) and chitooligosaccharides (COS), which are Maillard reaction products (LC-MRPs), during refrigerated storage. RESULTS: The results of microbiological analysis and confocal laser scanning microscope (CLSM) revealed that LC-MRPs could retard microbial growth effectively. Compared with control, other treated groups could strongly retard the increase in the thiobarbituric acid (TBA) value, the K-value and the total volatile basic nitrogen (TVB-N) value, and also inhibited the softening of texture and the accumulation of biogenic amines in fish. The results of low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) indicate that LC-MRPs could delay the water migration of fillets and increase water holding capacity (WHC). Through sensory evaluation, the application of LC-MRPs increased the shelf-life of refrigerated sea bass fillets for another 9 days. CONCLUSION: Maillard reaction products derived from chitooligosaccharides and ε-polylysine have strong potential for preserving sea bass. © 2022 Society of Chemical Industry.


Assuntos
Bass , Animais , Bass/microbiologia , Polilisina , Produtos Finais de Glicação Avançada , Água , Armazenamento de Alimentos , Conservação de Alimentos/métodos
3.
Food Chem ; 401: 134186, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36115233

RESUMO

This paper developed novel fermented stinky sea bass (FSSB) products and reports the first analysis of its taste active compounds, flavor compounds, and quality. The FSSB with Xian Hen stinky tofu (F-XH) had the best sensory quality. After fermentation, the texture of FSSB improved, and the umami amino and sweet amino acid contents significantly increased, whereas that of the bitter amino acids decreased. Moreover, the IMP content and EUC in FSSB increased significantly. Of the six key volatile flavor compounds distinguished, the key volatile flavor compounds of F-XH are Ethyl Acetate, Propan-2-ol, alpha-pinene, 2-methylbutanal, acetol, 4-Methylpentan-2-one. Ethyl Acetate and 2-propanol were thought to give F-XH its unique wine flavor after cooking. The quality evaluation results demonstrated that the six FSSB complied with the Chinese Standard (GB10136-2015) (2015) animal aquatic products. Six types of FSSB products with unique flavors were developed, and a reference was provided for their industrial application.


Assuntos
Bass , Compostos Orgânicos Voláteis , Animais , Feminino , Paladar , Compostos Orgânicos Voláteis/química , Inosina Monofosfato , Galinhas , 2-Propanol , Fermentação , Aminoácidos
4.
Artigo em Inglês | MEDLINE | ID: mdl-36306998

RESUMO

The enteric morphology, enteric microbiota structure and serum metabolomics of M. salmoides before and after infected by A. hydrophila were analysed to explore the pathogenic mechanism of A. hydrophila infection in M. salmoides. The results revealed that, after the infection of A. hydrophila, the villus boundary of largemouth bass became less obvious; the relative abundance of Proteobacteria and decreasing relative abundance of Tenericutes were increasing; genera relative abundance of putatively beneficial bacteria (Mycoplasma) were decreasing, whereas the genus Aeromonas increased after infection; serum metabolomic analysis showed that infection with A. hydrophila caused disorder to the metabolic processes of largemouth bass, particularly amino acid metabolism, and caused inflammation; several potential pathogen infection-related and significantly differential intestinal microbiota-related metabolite markers were identified, such as 6-hydroxy-5-methoxyindole glucuronide, zalcitabine, bilirubin, aciclovir. This study may provide new insights into the potential association between enteric microbiota and serum metabolism and the pathogenic mechanism of M. salmoides infected by A. hydrophila, providing a scientific basis for disease control in largemouth bass breeding.


Assuntos
Aeromonas , Bass , Microbioma Gastrointestinal , Animais , Aeromonas hydrophila
5.
Artigo em Inglês | MEDLINE | ID: mdl-36347467

RESUMO

This study evaluated whether different parameters describing cardiovascular function, energy metabolism, oxygen transport and oxidative stress were related to the critical thermal maximum (CTMAX) of European seabass (Dicentrarchus labrax) and if there were differential changes in these parameters during and after heat shock in animals with different CTMAX in order to characterize which physiological features make seabass vulnerable to heat waves. Seabass (n = 621) were tested for CTMAX and the physiological parameters were measured in individuals with good or poor temperature tolerance before and after a heat shock (change in temperature from 15 °C to 28 °C in 1.5 h). Fish with good thermal tolerance had larger ventricles with higher maximal heart rate during the heat shock than individuals with poor tolerance. Furthermore, they initially had a high ventricular Ca2+-ATPase activity, which was reduced to a similar level as in fish with poor tolerance following heat shock. The activity of heart lactate dehydrogenase increased in fish with high tolerance, when they were exposed to heat shock, while the aerobic enzyme activity did not differ between groups. The tolerant individuals had smaller red muscle fibers with higher myoglobin content than the poorly tolerant ones. The poorly tolerant individuals had higher hematocrit, which increased with heat shock in both groups. The poorly tolerant individuals had also higher activity of enzymes related to oxidative stress especially after heat shock. In general, CTMAX was not depending on merely one physiological factor but several organ and cellular parameters were related to the CTMAX of seabass and when working in combination they might protect the highly tolerant seabass from future heat waves.


Assuntos
Bass , Termotolerância , Animais , Coração , Ventrículos do Coração , Oxigênio
6.
Front Endocrinol (Lausanne) ; 13: 984081, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36339398

RESUMO

The leopard coral trout generally exhibited numerous round, minute blue spots covering its head (about the size of nostril) and body (except ventral side). This is a characteristic that distinguishes them from similar species. Recently, however, we found the leopard coral trout with black spots. Here, the distribution and ultrastructure of chromatophores in the blue and black spots were investigated with light and transmission electron microscopies. The results showed that in the blue spots, two types of chromatophores are present in the dermis, with the light-reflecting iridophores located in the upper layer and the aggregated light-absorbing melanophores in the lower layer. Black spots have a similar chromatophore composition, except that the melanosomes within the melanophores disperse their dendritic processes to encircle the iridophores. Interestingly, after the treatment of forskolin, a potent adenylate cyclase activator, the blue spots on the body surface turned black. On the other hand, using the skin preparations in vitro, the electrical stimulation and norepinephrine treatment returned the spots to blue color again, indicating the sympathetic nerves were involved in regulating the coloration of blue spots. Taken together, our results revealed that the blue spots of the leopard coral trout can change color to black and vice versa, resulting from the differences in the distribution of melanosomes, which enriches our understanding of the body color and color changes of fishes.


Assuntos
Antozoários , Bass , Cromatóforos , Panthera , Animais , Cromatóforos/ultraestrutura , Truta
7.
Int J Mol Sci ; 23(21)2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36362422

RESUMO

European sea bass (Dicentrarchus labrax) are a major aquaculture species that live in habitats with fluctuating salinities that are sometimes higher than in seawater (SW). Atlantic and West-Mediterranean genetic lineages were compared regarding intestinal neuropeptide receptor expression in SW (36%) and following a two-week transfer to hypersalinity (HW, 55%). Phylogenetic analysis revealed seven neuropeptide receptors belonging to the arginine vasotocine (AVTR) family and two isotocin receptors (ITR). Among AVTR paralogs, the highest mRNA levels were recorded for v1a2, with a two- to fourfold upregulation in the European sea bass intestinal sections after transfer of fish to HW. Principal component analysis in posterior intestines showed that v1a2 expression grouped together with the expression and activity of main ion transporters and channels involved in solute-coupled water uptake, indicating a possible role of this receptor in triggering water absorption. v1a1 expression, however, was decreased or did not change after transfer to hypersaline water. Among ITR paralogs, itr1 was the most expressed paralog in the intestine and opposite expression patterns were observed following salinity transfer, comparing intestinal sections. Overall, different expression profiles were observed between genetic lineages for several analyzed genes which could contribute to different osmotic stress-related responses in D. labrax lineages.


Assuntos
Bass , Animais , Bass/genética , Bass/metabolismo , Filogenia , Brânquias/metabolismo , Intestinos , Água do Mar , Água/metabolismo
8.
Front Cell Infect Microbiol ; 12: 1042977, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36325466

RESUMO

The largemouth bass (Micropterus salmoides) is an economically important aquaculture species in China, and its production has increased rapidly in recent years. Although Shewanella putrefaciens is known to infect several fish species, its role in infecting M. salmoides is relatively unknown. Here, we isolated a gram-negative bacterial strain (termed XX2021) from farmed largemouth bass. Based on the results of 16S rRNA sequencing and phylogenetic analyses, the isolate was identified as S. putrefaciens. The virulence of XX2021 was dependent on water temperature, such as the LD50 values were 4.21×104, 7.26×105, and 2.47×106 CFU/g fish weight at 10°C, 18°C, and 25°C, respectively. Four virulent genes-including dksA, hem, lonR, and fur-were screened through a PCR assay. The results of an antibiotic resistance test showed that XX2021 was sensitive to kanamycin, cefotaxime, doxycycline, sulfamethoxazole, florfenicol, tetracycline, and gentamicin; showed intermediate susceptibility to streptomycin, ampicillin, and norfloxacin; and was resistant to nalidixic acid and penicillin. XX2021-infected fish showed clinical symptoms typical of S. putrefaciens infection. In addition, we re-isolated XX2021 from infected fish and confirmed its identity using 16S rRNA sequencing. Histopathological changes were observed in the intestine, head kidney, spleen, and liver of diseased fish. This study presents the first report of the pathogenic effects of S. putrefaciens in farmed largemouth bass. Our findings may help develop effective disease control strategies for aquaculture fish and prevent disease outbreaks under low water temperatures.


Assuntos
Bass , Doenças dos Peixes , Shewanella putrefaciens , Animais , Shewanella putrefaciens/genética , RNA Ribossômico 16S/genética , Filogenia , Doenças dos Peixes/microbiologia , Água
9.
Front Immunol ; 13: 997985, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36189250

RESUMO

Cottonseed protein concentrate (CPC) has been proven to partially replace fishmeal without adverse effects on fish growth performance, while little information is known about the effects on liver health during bacterial infection. In the present study, 15% CPC was included into the diet of juvenile largemouth bass (32.12 ± 0.09g) to replace fishmeal for 8 weeks, with fish growth potential and hepatic inflammatory responses during Nocardia seriolae (N. seriolae) infection systemically evaluated. After adaptation to dietary CPC inclusion, largemouth bass even exhibited better growth potential with higher SGR and WGR during the last three weeks of whole feeding trial, which was accompanied with higher phosphorylation level of TOR signaling and higher mRNA expression level of myogenin (myog). At the end of 8-weeks feeding trial, the histological structure of largemouth bass liver was not significantly affected by dietary CPC inclusion, accompanied with the similar expression level of genes involved in innate and adaptive immunity and comparable abundance of T cells in bass liver. N.seriolae infection induced the pathological changes of bass liver, while such hepatic changes were more serious in CPC group than that in FM group. Additionally, RT-qPCR results also suggested that largemouth bass fed with CPC experienced much higher inflammatory potential both in liver and gill during N. seriolae infection, which was accompanied with higher expression level of genes involved in pyroptosis. Therefore, this study demonstrated that the application of CPC in largemouth bass diet should be careful, which may induce higher inflammatory potential during N. seriolae infection.


Assuntos
Bass , Nocardiose , Animais , Bass/genética , Óleo de Sementes de Algodão , Proteínas na Dieta , Miogenina , RNA Mensageiro
10.
Zool Res ; 43(6): 952-965, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36208122

RESUMO

Pseudomonas plecoglossicida is the pathogen responsible for visceral white spot disease in large yellow croaker (Larimichthys crocea) and orange-spotted grouper (Epinephelus coioides). Previously, RNA sequencing showed that P. plecoglossicida flgK gene expression was significantly up-regulated in orange-spotted grouper spleens during infection. To explore the role of flgK in P. plecoglossicida pathogenicity, RNA interference (RNAi) was performed to silence the P. plecoglossicida flgK gene, and the mutant (flgK-RNAi strain) with the best silencing efficiency (89.40%) was chosen for further study. Results showed that flgK gene silencing significantly attenuated P. plecoglossicida motility, adhesion, and biofilm formation. Compared to those fish infected with the wild-type strain of P. plecoglossicida, orange-spotted grouper infected with the flgK-RNAi strain showed a 55% increase in the survival rate and a one-day delay in time of first death, with fewer pathogens in the spleen and fewer white spots on the spleen surface. RNAi of flgK significantly affected the transcriptome and metabolome of the spleen in infected orange-spotted grouper. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the C-type lectin receptor signaling pathway was the most significantly changed immune-related pathway and the mitogen-activated protein kinase (MAPK) signaling pathway was related to multiple immune-related pathways. Furthermore, arginine biosynthesis and glycerophospholipid metabolism were the most significantly changed metabolism-related pathways. These findings suggest that flgK is a virulence gene of P. plecoglossicida. Furthermore, flgK appears to be involved in the regulation of motility, adhesion, and biofilm formation in P. plecoglossicida, as well as in the regulation of inflammatory and immune responses of orange-spotted grouper to P. plecoglossicida infection.


Assuntos
Bass , Perciformes , Infecções por Pseudomonas , Animais , Arginina/genética , Proteínas de Bactérias/genética , Bass/genética , Bass/metabolismo , Proteínas de Peixes/genética , Glicerofosfolipídeos , Interações Hospedeiro-Patógeno/genética , Imunidade Inata , Lectinas Tipo C/genética , Lectinas Tipo C/metabolismo , Proteínas Quinases Ativadas por Mitógeno/genética , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Perciformes/genética , Perciformes/metabolismo , Pseudomonas , Infecções por Pseudomonas/veterinária , Transcriptoma , Virulência/genética
11.
Front Immunol ; 13: 985291, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36203610

RESUMO

TRIM (tripartite motif) proteins have been demonstrated to exert critical roles in host defense against different microbial pathogens. Among them, TRIM23 acts as an important regulatory factor in antiviral immune and inflammatory responses, but the roles of fish TRIM23 against virus infection still remain largely unknown. Here, we investigated the characteristics of TRIM23 homolog from orange spotted grouper (Epinephelus coioides) (EcTRIM23). EcTRIM23 encoded a 580 amino acid peptide, which shared 93.1%, 89.73% and 86.36% identity with golden perch (Perca flavescens), zebrafish (Danio rerio) and human (Homo sapiens), respectively. The transcription levels of EcTRIM23 were significantly up-regulated in response to Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV) infection. EcTRIM23 overexpression in vitro significantly inhibited RGNNV and SGIV replication, evidenced by the delayed cytopathic effect (CPE) progression and the decreased expression of viral core genes. EcTRIM23 significantly increased the expression levels of interferon (IFN) related signaling molecules and pro-inflammatory cytokines, as well as the promoter activities of IFN and NF-κB, suggesting that EcTRIM23 exerted antiviral function by positively regulating host IFN response. Exogenous EcTRIM23 exhibited either diffuse or aggregated localization in grouper cells. After co-transfection, TANK binding kinase 1 (TBK1), TNF receptor associated factor (TRAF) 3 and TRAF4, TRAF5 and TRAF6 were found to interact with EcTRIM23 in grouper cells. Moreover, these proteins could be recruited and co-localized with EcTRIM23 in vitro. Together, our results demonstrated that fish TRIM23 exerted antiviral activity against fish viruses by interacting with multiple host proteins to regulate immune responses.


Assuntos
Bass , Infecções por Vírus de DNA , Doenças dos Peixes , Iridovirus , Nodaviridae , Ranavirus , Aminoácidos/genética , Animais , Antivirais/farmacologia , Infecções por Vírus de DNA/veterinária , Proteínas de Peixes/metabolismo , Proteínas de Ligação ao GTP , Humanos , Imunidade Inata/genética , Interferons/metabolismo , NF-kappa B/metabolismo , Nodaviridae/fisiologia , Ranavirus/fisiologia , Alinhamento de Sequência , Fator 4 Associado a Receptor de TNF/genética , Fator 4 Associado a Receptor de TNF/metabolismo , Fator 5 Associado a Receptor de TNF/genética , Fator 6 Associado a Receptor de TNF/metabolismo , Peixe-Zebra/genética
12.
Int J Biol Macromol ; 222(Pt A): 1037-1047, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36181882

RESUMO

A 56-day feeding trial was conducted to assess the effects of different viscous guar gum on the growth, intestinal flora, and intestinal health of Micropterus salmoides. Four practical diets with 42.5 % crude protein and 13.7 % crude lipid were formulated to contain 8 % cellulose and three different viscosities (2500, 5200, and 6000 mPa·s) of guar gum. Dietary guar gum inhibits fish growth and feed utilization, decreases the α-diversity of the intestinal flora, and negatively alters the intestinal flora structure and metabolite composition. High viscous guar gum down-regulated the intestinal tight junction, anti-inflammatory, and anti-apoptotic related gene's expression, decreased digesta butyrate/histamine ratio; and increased the abundance of Plesiomonas shigelloides. These results suggest that dietary guar gum adversely affects intestinal health by disrupting intestinal flora structure and metabolite composition, and that viscosity should be considered when using guar gum as a binder in aquafeeds.


Assuntos
Bass , Microbioma Gastrointestinal , Animais , Viscosidade , Galactanos/química , Gomas Vegetais/farmacologia , Gomas Vegetais/química , Mananas/química , Fibras na Dieta
13.
Front Immunol ; 13: 973422, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36275642

RESUMO

To better understand the response of largemouth bass (Micropterus salmoides) to Micropterus salmoides rhabdovirus (MSRV) infection, we investigated the intestinal bacterial flora and transcriptome profile of fish at 72 hours post-infection (hpi). Total of 1574 differentially expressed genes (DEGs) were identified in largemouth bass spleen following MSRV infection, including 573 upregulated and 1001 downregulated genes. KEGG and GO enrichment analysis revealed that upregulated genes were enriched in certain antiviral related signaling pathway, including NOD-like receptor (NLR), RIG-I like receptors (RLR) and regulation of the interferon (IFN)-γ-mediated signaling pathway, whereas some immune-related DEGs enriched in focal adhesion (FA) and ECM-receptor interaction(ECM-RI) were downregulated, as well as genes associated with metabolic processes, such as peroxisome proliferator-activated receptors (PPAR), adipocytokine signaling pathway, Glycerolipid and Retinol metabolism. Furthermore, the principal component analysis (PCA) and phylogenetic analysis revealed that MSRV infection significantly affected the microbiota of largemouth bass intestine; the LEfSe analysis showed that relative abundances of Streptococcus were significantly increased, while the content of Akkermansia, Enterococcus and Lactobacillus were remarkably decreased in the fish intestine following MSRV infection. Additionally, a high correlation was determined between the expressions of interferon-related upregulated genes and the relative abundance of Streptococcus by redundancy analysis (RDA). These results collectively illustrated that intestinal microbiota composition might be associated with the immune-related gene expression in largemouth bass in response to MSRV infection.


Assuntos
Bass , Infecções por Rhabdoviridae , Rhabdoviridae , Animais , Bass/genética , Transcriptoma , RNA Ribossômico 16S , Receptores Ativados por Proliferador de Peroxissomo , Filogenia , Vitamina A , Interferons/genética , Proteínas NLR/genética , Antivirais , Adipocinas/genética
14.
Int J Mol Sci ; 23(19)2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36232462

RESUMO

Environmental effects and, particularly, temperature changes have been demonstrated to influence the activity, function, and well-being of teleosts. Temperature may change seasonally in the wild, and in captivity under aquaculture operations. Moreover, climate change is expected to shift temperature profiles worldwide. MicroRNAs (miRNA) are important temperature-sensitive gene-expression regulators acting at the post-transcriptional level. They are known to be key regulators in development, reproduction, and immune responses. Therefore, early larval development of the European sea bass (Dicentrarchus labrax), one of the most extensively cultured species in Mediterranean aquaculture, was investigated at early rearing temperatures, i.e., 15, 17.5, and 20 °C, in regard to the impact of temperatures on miRNAs through sncRNA high-throughput sequencing but also at the phenotypic level in terms of growth, sex, vision, and skeletal deformities. Expression profiling revealed stage- and temperature-specific miRNA expression targeting genes with roles in reproduction and immune response mainly at the flexion and all-fins stages. Similar stage- and temperature-specific results were also observed concerning the number of rod cells and lower jaw elongation. The present work presents for the first time highly promising results on the influence of early rearing temperature at the post-transcriptional level during European sea bass development, with a putative impact on reproduction and immune response, as well as regarding teleost vision and larval development.


Assuntos
Bass , MicroRNAs , Pequeno RNA não Traduzido , Animais , Aquicultura , Bass/genética , Bass/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Pequeno RNA não Traduzido/metabolismo , Temperatura
15.
Int J Mol Sci ; 23(19)2022 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-36232658

RESUMO

Toll-like receptor (TLR) is a cluster of type I transmembrane proteins that plays a role in innate immunity. Based on the marbled rockfish (Sebastiscus marmoratus) genome database, this study used bioinformatics methods to identify and analyze its TLR gene family members. The results showed that there were 11 TLR gene family members in Sebastiscus marmoratus (SmaTLR), which could be divided into five different subfamilies. The number of amino acids encoded by the Smatlr genes ranged from 637 to 1206. The physicochemical properties of the encoded proteins of different members were also computed. The results of protein structure prediction, phylogenetic relation, and motif analysis showed that the structure and function of the SmaTLRs were relatively conserved. Quantitative Real-Time PCR (qRT-PCR) analysis revealed the expression patterns of SmaTLRs in the gill, liver, spleen, head kidney, kidney, and intestine. SmaTLRs were widely detected in the tested tissues, and they tended to be expressed higher in immune-related tissues. After polyriboinosinic polyribocytidylic acid (poly(I:C)) challenge, SmaTLR14, SmaTLR3, SmaTLR5S, SmaTLR7, and SmaTLR22 were significantly upregulated in the spleen or liver. The results of this study will help to understand the status of TLR gene family members of marbled rockfish and provide a basis for further study of the functional analysis of this gene family.


Assuntos
Bass , Perciformes , Aminoácidos/metabolismo , Animais , Bass/metabolismo , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Imunidade Inata/genética , Perciformes/metabolismo , Filogenia , Poli I-C , Receptores Toll-Like/metabolismo
16.
Int J Mol Sci ; 23(19)2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-36232925

RESUMO

Vibrio harveyi is one of the most serious bacterial pathogens to aquatic animals worldwide. Evidence is mounting that coinfections caused by multiple pathogens are common in nature and can alter the severity of diseases in marine animals. However, bacterial coinfections involving V. harveyi have received little attention in mariculture. In this study, the results of pathogen isolation indicated that bacterial coinfection was a common and overlooked risk for hybrid groupers (♀ Epinephelus polyphekadion × â™‚ E. fuscoguttatus) reared in an industrialized flow-through pattern in Hainan Province. The artificial infection in hybrid groupers revealed that coinfections with V. harveyi strain GDH11385 (a serious lethal causative agent to groupers) and other isolated pathogens resulted in higher mortality (46.67%) than infection with strain GDH11385 alone (33.33%), whereas no mortality was observed in single infection with other pathogens. Furthermore, the intestine, liver and spleen of hybrid groupers are target organs for bacterial coinfections involving V. harveyi. Based on the infection patterns found in this study, we propose that V. harveyi may have a specific spatiotemporal expression pattern of virulence genes when infecting the host. Taken together, bacterial coinfection with V. harveyi is a neglected high-risk lethal causative agent to hybrid groupers in the industrialized flow-through aquaculture systems in Hainan Province.


Assuntos
Bass , Coinfecção , Doenças dos Peixes , Vibrioses , Vibrio , Animais , Aquicultura , Bass/genética , Coinfecção/veterinária , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/microbiologia , Prevalência , Vibrio/genética , Vibrioses/epidemiologia , Vibrioses/microbiologia , Vibrioses/veterinária
17.
Environ Sci Technol ; 56(20): 14375-14386, 2022 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-36197672

RESUMO

Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides) exposed to a potent synthetic estrogen (17α-ethinylestradiol, EE2) at a low (EE2Low, 0.87 ng/L) or high (EE2High, 9.08 ng/L) dose for 4 weeks, followed by transfer to clean water and injection with an LD40 dose of the Gram-negative bacteria Edwardsiella piscicida. Unexpectedly, this prior exposure to EE2High significantly increased survivorship at 10 d post-infection compared to solvent control or EE2Low-exposed, infected fish. Both prior exposure and infection with E. piscicida led to significantly reduced hepatic glycogen levels, indicating a stress response resulting in depletion of energy stores. Additionally, pathway analysis for liver and spleen indicated differentially expressed genes associated with immunometabolic processes in the mock-injected EE2High treatment that could underlie the observed protective effect and metabolic shift in EE2High-infected fish. Our results demonstrate that exposure to a model EEDC alters metabolism and immune function in a fish species that is ecologically and economically important in North America.


Assuntos
Infecções Bacterianas , Bass , Animais , Bass/genética , Bass/metabolismo , Etinilestradiol/metabolismo , Etinilestradiol/toxicidade , Glicogênio Hepático/metabolismo , Solventes , Água/metabolismo
18.
Mar Biotechnol (NY) ; 24(6): 1084-1093, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36227511

RESUMO

The nervous necrosis virus (NNV) causes the viral nervous necrosis (VNN) disease in aquatic animals and has been a major threat in aquaculture. Thus, it is essential for the development of a prevention method to minimize economic losses caused by NNV such as the identification of NNV resistance genes and application of these genes in molecular breeding to increase disease resistance. gab3 is an important NNV resistance gene in Asian seabass. However, the mechanism of gab3 in NNV resistance has not been elucidated. In this study, knockdown of gab3 in NNV-infected Asian seabass cells resulted in a significant decrease in viral RNA and virus titers. Knockout of gab3 in zebrafish led to an increased survival rate and resistant time after NNV infection. Cellular localization of the GAB3 and NNV by immunofluorescence staining showed that the GAB3 was translocated from the nucleus to the cytoplasm, and finally reached the cell membrane of SB cells after 48 h post NNV infection. Our study suggests that gab3 plays an important role in NNV replication and silencing gab3 can inhibit virus replication.


Assuntos
Bass , Doenças dos Peixes , Nodaviridae , Perciformes , Infecções por Vírus de RNA , Animais , Infecções por Vírus de RNA/genética , Peixe-Zebra , Nodaviridae/fisiologia , Replicação Viral , Necrose , Bass/genética
19.
Ecotoxicol Environ Saf ; 247: 114203, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36279634

RESUMO

Little is known about the effects of oxytetracycline (OTC) on marine fish. Using juvenile pearl gentian grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂), we investigated the effects of 56 days dietary oxytetracycline (OTC; OTCD) exposure (80 mg/kg body weight/day) on fish growth performance, intestinal health and antibiotic residue. Meanwhile, the alteration of intestinal health status and antibiotic residue after 7 (O7) or 14 days (O14) OTC withdrawal treatment were explored. We also assessed the potential human health risks based on target hazard quotient (THQ). The results showed that OTC exposure had no effects on growth rate parameters but caused liver and intestine atrophy. OTC exposure impaired the intestinal health by distorting intestinal morphological features, inducing oxidative stress, repressing immune function, triggering NF-κB-mediated inflammatory response and apoptosis, while 7 days OTC withdrawal treatment improved intestinal health status and 14 days OTC withdrawal treatment further improved it. In addition, the order of OTC residue in tested tissues after OTC exposure was: liver > kidney > spleen > muscle > intestine. OTC residue was significantly decreased in all tissues along with the increasing of withdrawal treatment period. Adults and children consuming the edible tissues (muscle) of OTC-treated or withdrawal-treated pearl gentian grouper would not experience any previous health risk as the THQ < 1 in all group.


Assuntos
Bass , Oxitetraciclina , Humanos , Animais , Criança , Oxitetraciclina/toxicidade , Bass/fisiologia , Antibacterianos , Ração Animal/análise , Intestinos
20.
Parasite ; 29: 48, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36282090

RESUMO

Pseudorhabdosynochus suratthaniensis n. sp. is described from the gills of Cephalopholis argus; P. cephalopholi n. sp., from the gills of C. sonnerati; and P. samaesarnensis n. sp., from the gills of Epinephelus lanceolatus. These fish were all caught in the Gulf of Thailand. Pseudorhabdosynochus suratthaniensis n. sp. is distinguished from congeneric species by the structure of its sclerotized vagina, which has a wide sclerotized trumpet and a single large primary chamber, and by the number of rows of rodlets in each of its squamodiscs. Pseudorhabdosynochus cephalopholi n. sp. is also distinguished by the structure of its sclerotized vagina that, like the P. suratthaniensis n. sp., has a sclerotized trumpet, but it also has a long coiled or curved primary canal near its midlength, and a distal part with a primary chamber and a secondary chamber communicating with the primary chamber through a short secondary canal. In addition, P. cephalopholi n. sp. is distinguished by some sclerotized organs (ventral and dorsal hamuli, ventral bar, and quadriloculate organ) with different lengths, and by the number of rows of rodlets in each of its squamodiscs. Pseudorhabdosynochus samaesarnensis n. sp. is distinguished by its sclerotized vagina that has an anterior cup-shaped trumpet and a short straight or curved primary canal. For Thailand, these are the first species of Pseudorhabdosynochus described from species of Cephalopholis and the second species of Pseudorhabdosynochus described from Epinephelus.


Title: Trois nouvelles espèces de Pseudorhabdosynochus (Monogenea, Diplectanidae), parasites de plusieurs espèces de Cephalopholis et Epinephelus (Perciformes, Serranidae) de Thaïlande. Abstract: Pseudorhabdosynochus suratthaniensis n. sp. est décrit à partir des branchies de Cephalopholis argus, P. cephalopholi n. sp. des branchies de C. sonnerati, et P. samaesarnensis n. sp. des branchies d'Epinephelus lanceolatus. Ces poissons ont tous été pêchés dans le golfe de Thaïlande. Pseudorhabdosynochus suratthaniensis n. sp. se distingue des espèces congénères par la structure de son vagin sclérifié, qui possède une large trompette sclérifiée et une seule grande chambre primaire, et par le nombre des rangées de bâtonnets dans chacun de ses squamodisques. Pseudorhabdosynochus cephalopholi n. sp. se distingue également par la structure de son vagin sclérifié qui, comme P. suratthaniensis n. sp., a une trompette sclérifiée, mais a également un long canal primaire enroulé ou incurvé près de sa mi-longueur et une partie distale avec une chambre primaire et une chambre secondaire communiquant avec la chambre primaire par un court canal secondaire. De plus, P. cephalopholi n. sp. se distingue par certains organes sclérifiés (hamuli ventraux et dorsaux, barre ventrale et organe quadriloculé) de longueurs différentes, et par le nombre des rangées de bâtonnets dans chacun de ses squamodisques. Pseudorhabdosynochus samaesarnensis n. sp. se distingue par son vagin sclérifié qui a une trompette antérieure en forme de coupe et un court canal primaire droit ou courbe. Pour la Thaïlande, il s'agit de la première espèce de Pseudorhabdosynochus décrite à partir d'espèces de Cephalopholis et de la deuxième espèce de Pseudorhabdosynochus décrite à partir d'Epinephelus.


Assuntos
Bass , Doenças dos Peixes , Perciformes , Trematódeos , Infecções por Trematódeos , Animais , Feminino , Tailândia , Brânquias , Infecções por Trematódeos/veterinária
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