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1.
Int J Oral Maxillofac Implants ; 35(1): 187-195, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31923301

RESUMO

PURPOSE: To evaluate histomorphometrically early healing at implants placed in sites prepared with either a sonic device or conventional drills. MATERIALS AND METHODS: Sixteen volunteer patients were recruited. Two titanium mini-implants were placed in the distal segments of the maxilla in recipient sites prepared with either a sonic device or conventional drills. Biopsy specimens containing the mini-implants were retrieved after 2 weeks in eight patients, and after 6 weeks in the other eight patients. Histomorphometric analyses were performed. RESULTS: Histologic slides were available from seven patients for both 2-week and 6-week periods. After 2 weeks of healing, small amounts of new bone were found in contact with the implant surface, with 5.5% ± 7.3% and 3.8% ± 10.0% at the sonic and drill groups, respectively. After 6 weeks of healing, new bone was 46.9% ± 15.5% at the sonic group, and 46.4% ± 14.9% at the drill group. None of the differences was statistically significant. CONCLUSION: The percentage of new bone in contact with the implant surface was similar in the sonic and drill groups.


Assuntos
Implantes Dentários , Boca , Implantação Dentária Endo-Óssea , Humanos , Maxila , Osseointegração , Propriedades de Superfície , Titânio
2.
J Prosthodont ; 29(1): 12-18, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31660673

RESUMO

PURPOSE: To compare abutment displacement between the virtual, customized abutment that was designed on a cast and the customized abutment prepared in the oral cavity. MATERIALS AND METHODS: Eleven patients were selected for a single posterior implant prosthetic treatment. The impression was obtained using the closed tray impression method with a vinyl polysiloxane material using a custom tray. The standard tessellation language files of the customized abutment that was designed using the computer-aided design system and acquired with an intraoral scanner in the oral cavity were superimposed and analyzed for distance and angle displacement using the three-dimensional inspection analysis program (Geomagic Control X). In the statistical analysis, distance and angle displacement values were analyzed with the Kruskal-Wallis H test (α = 0.05), and a post hoc comparison was performed using the Mann-Whitney U-test and Bonferroni correction method. RESULTS: The mean distance and angle displacement of the 15 customized abutments were 89.52 ± 66.86 µm and 0.83 ± 1.21°, respectively. There were significant differences in distance displacement (p < 0.001), and angle displacement (p < 0.001) among the 15 customized abutments, and there were no significant differences in angle displacement along the 4 directions (p = 0.735). CONCLUSIONS: The displacement values of the customized abutments evaluated in the oral cavity differed significantly from patient to patient.


Assuntos
Projeto Auxiliado por Computador , Dente Suporte , Humanos , Boca
3.
J Oral Pathol Med ; 49(1): 14-20, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31424136

RESUMO

BACKGROUND: Solitary fibrous tumour is an unusual neoplasm of the oral cavity that is sometimes not clinically distinguishable from other lesions. The purpose of the present study was to review the clinical, microscopic and molecular aspects of malignant and benign solitary fibrous tumour of the oral cavity currently available in literature. METHODS: For our review, an electronic search was performed using PubMed, Scopus, Ovid/MedLine, Web of science and ProQuest Dissertations and Theses Global database. RESULTS: A total of 74 publications reporting 150 cases were included. Oral solitary fibrous tumours are most frequently described as submucosal, well-circumscribed, asymptomatic nodule, more prevalent in females in their fourth to fifth decades of life. Buccal mucosa is the most commonly affected site by the benign tumour variant, whereas the tongue is the most common location affected by the malignant form of the neoplasm. Most of the lesions were treated by conservative surgery. One recurrent malignant tumour and one metastasis are reported. CONCLUSION: Asymptomatic normal-coloured submucosal nodules located in the buccal mucosa and tongue in adult patients are suggestive of oral solitary fibrous tumour, but only a careful microscopic examination can differentiate benign from malignant variants and the use of immunohistochemistry (CD34, Bcl-2, CD99 and STAT6), and cytogenetic studies (NAB2-STAT6) contribute significantly to confirm the diagnosis of solitary fibrous tumour in difficult cases.


Assuntos
Recidiva Local de Neoplasia , Tumores Fibrosos Solitários , Biomarcadores Tumorais , Humanos , Imuno-Histoquímica , Boca
4.
Int J Radiat Biol ; 96(1): 100-111, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-29447591

RESUMO

Purpose: Characterization of a novel partial-body irradiation (PBI) shielding strategy in nonhuman primates (NHP; rhesus macaques), aimed at protecting the oral cavity, with respect to various gastrointestinal acute radiation syndrome (GI-ARS) syndrome parameters as well as buccal ulceration development.Materials and methods: NHPs were irradiated using a Cobalt-60 gamma source, in a single uniform dose, ranging from 9-13 Gy and delivered at 0.60-0.80 Gy min-1. Animals were either partially shielded via oral cavity shielding (PBIOS) or underwent total-body irradiation (TBI).Results: Clinical manifestations of GI-ARS, and also radiation-induced hematology and clinical chemistry changes, following PBIOS were comparable to the PBI NHP GI-ARS model utilizing shielding of the distal pelvic limbs and were significantly milder than TBI at similar radiation doses. Nadir citrulline levels were comparable between PBIOS and TBI but signs of recovery appeared earlier in PBIOS-treated animals. The PBIOS model prevented oral mucositis, whereas the TBI model presented buccal ulcerations at all tested radiation dose levels.Conclusions: Taken together, these results suggest that the PBIOS model is a suitable alternative to traditional PBI. For GI-ARS investigations requiring orally administered medical countermeasures, PBIOS confers added value due to the prevention of oral mucositis over traditional PBI.


Assuntos
Boca/efeitos da radiação , Proteção Radiológica/métodos , Síndrome Aguda da Radiação/sangue , Síndrome Aguda da Radiação/etiologia , Síndrome Aguda da Radiação/patologia , Animais , Citrulina/sangue , Radioisótopos de Cobalto/efeitos adversos , Raios gama/efeitos adversos , Macaca mulatta , Masculino , Análise de Sobrevida , Úlcera/sangue , Úlcera/etiologia , Úlcera/patologia
5.
Immunology ; 159(1): 26-38, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31777068

RESUMO

Mucosal tissues contain distinct microbial communities that differ drastically depending on the barrier site, and as such, mucosal immune responses have evolved to be tailored specifically for their location. Whether protective or regulatory immune responses against invading pathogens or the commensal microbiota occur is controlled by local mononuclear phagocytes (MNPs). Comprising macrophages and dendritic cells (DCs), the functions of these cells are highly dependent on the local environment. For example, the intestine contains the greatest bacterial load of any site in the body, and hence, intestinal MNPs are hyporesponsive to bacterial stimulation. This is thought to be one of the major mechanisms by which harmful immune responses directed against the trillions of harmless bacteria that line the gut lumen are avoided. Regulation of MNP function by the microbiota has been characterized in the most depth in the intestine but there are several mucosal sites that also contain their own microbiota. In this review, we present an overview of how MNP function is regulated by the microbiota at mucosal sites, highlighting recent novel pathways by which this occurs in the intestine, and new studies elucidating these interactions at mucosal sites that have been characterized in less depth, including the urogenital tract.


Assuntos
Imunidade nas Mucosas , Microbiota/imunologia , Sistema Fagocitário Mononuclear/imunologia , Membrana Mucosa/microbiologia , Simbiose/imunologia , Animais , Interações entre Hospedeiro e Microrganismos/imunologia , Humanos , Tolerância Imunológica , Intestinos/imunologia , Intestinos/microbiologia , Pulmão/microbiologia , Modelos Animais , Boca/imunologia , Boca/microbiologia , Membrana Mucosa/imunologia , Sistema Urogenital/imunologia , Sistema Urogenital/microbiologia
6.
Am J Orthod Dentofacial Orthop ; 156(6): 735-744, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31784007

RESUMO

INTRODUCTION: In this pilot study, we aimed to determine qualitative and quantitative microbiological changes after the implementation of orthodontic appliances. METHODS: A total of 10 healthy patients aged 12-15 years were recruited who needed to undergo orthodontic treatment with buccal fixed appliances. Gingival conditions were assessed by the Gingival Index, Periodontal Screening Index, and Sulcus Bleeding Index. Microbiological samples were collected before and 1 week after the start of therapy at premolars and molars of the right upper quadrant. Bacterial species were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. RESULTS: The total number of bacteria increased. Six bacterial species were identified that are involved in the development of caries and other infectious processes. The bacteria selectively adapted more efficiently to the new oral milieu compared with the general oral microbial background. There was a significant increase in Streptococcus spp at the premolars and molars. In all individuals, symptoms of inflammation and gingivitis were detected as a response to the bacterial challenge. CONCLUSIONS: Orthodontic treatment induces significant changes in the oral microbial flora associated with gingivitis and an enhanced risk for cariogenic reactions within the first days of orthodontic treatment. To prevent or reduce infectious side effects, oral hygiene instructions and control of patients are necessary before and during the beginning of the therapy.


Assuntos
Bactérias , Gengivite , Boca , Aparelhos Ortodônticos Fixos , Adolescente , Criança , Humanos , Boca/microbiologia , Aparelhos Ortodônticos , Índice Periodontal , Projetos Piloto
7.
Khirurgiia (Mosk) ; (12): 18-27, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31825339

RESUMO

OBJECTIVE: To analyze own initial experience of transoral thyroid surgery. MATERIAL AND METHODS: There were 7 patients thyroid nodules who underwent surgery for the period from March 2018 to May 2019. All patients signed an informed consent to be included in the study. Surgical approach was performed through three incisions in the lower arch of the vestibule of the mouth with deployment of 10 mm endoscope and two 5 mm tools. Gas insufflation was used. All patients were females aged 43.3±11.8 years. Thyroidectomy was performed in 2 cases, hemithyroidectomy - in 5 patients. Dimensions of nodules varied from 10 to 42 mm. RESULTS: Mean time of hemithyroidectomy and thyroidectomy was 206.4±63.8 and 232±37.5 min, respectively. Papillary carcinoma was histologically verified in 1 case. Injuries of recurrent laryngeal nerve, postoperative hypocalcemia and local complications were absent. Drainage was not applied. Postoperative hospital-stay was 3.7±1.1 days. CONCLUSION: Transoral approach to the thyroid gland is technically feasible with standard endoscopic instruments, safe for important anatomical structures and more precise due to the optical capabilities of endoscopic equipment. Any types of procedures are possible. Undoubtedly, aesthetic outcome is also favorable.


Assuntos
Cirurgia Endoscópica por Orifício Natural/efeitos adversos , Cirurgia Endoscópica por Orifício Natural/métodos , Nódulo da Glândula Tireoide/cirurgia , Tireoidectomia/métodos , Adulto , Carcinoma Papilar/cirurgia , Estudos de Viabilidade , Feminino , Humanos , Pessoa de Meia-Idade , Boca/cirurgia , Neoplasias da Glândula Tireoide/cirurgia
8.
J Contemp Dent Pract ; 20(8): 963-969, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31797855

RESUMO

AIM: The aim of this study was to evaluate and compare two different one-stage full-mouth disinfection protocols in the treatment of chronic periodontitis by assessing dental plaque and tongue coat using BANA assay. MATERIALS AND METHODS: The present study was a prospective randomized clinical parallel arm study design including 40 healthy subjects randomly allocated into two groups, i.e., group A (Quirynen's protocol of one-stage full-mouth disinfection) and group B (Bollen's protocol of one-stage full-mouth disinfection). Subjects were assessed at baseline and six weeks using plaque index, gingival index, and sulcus bleeding index. Probing depth and relative clinical attachment level were also recorded at six weeks. Winkel tongue coat index and BANA were recorded at 8 weeks using subgingival plaque and tongue coat sample. RESULTS: Both group A and group B demonstrated statistically significant reduction in plaque index, gingival index, sulcus bleeding index, Winkel tongue coat index, reduction in probing depth, and gain in relative clinical attachment level on intragroup comparison. There was no significant difference in BANA assay score of subgingival plaque and tongue coat samples in between group A and group B. CONCLUSION: From the findings of this study, both Quirynen's protocol and Bollen's protocol of one-stage full-mouth disinfection are effective in plaque reduction and tongue coat reduction and achieve comparable clinical healing outcomes. CLINICAL SIGNIFICANCE: The difference in duration and mode of use of chlorhexidine as a chemical plaque control agent in the two treatment interventions of Quirynen's and Bollen's protocol of one-stage full-mouth disinfection did not demonstrate statistical significance in reducing sulcus bleeding index scores, reducing probing depths, and gain in relative clinical attachment levels.


Assuntos
Anti-Infecciosos Locais , Raspagem Dentária , Antissépticos Bucais , Anti-Infecciosos Locais/uso terapêutico , Índice de Placa Dentária , Desinfecção , Humanos , Boca/microbiologia , Estudos Prospectivos , Aplainamento Radicular
10.
Ned Tijdschr Tandheelkd ; 126(12): 653-656, 2019 Dec.
Artigo em Holandês | MEDLINE | ID: mdl-31840676

RESUMO

Scientific literature demonstrates relationships to exist between oral health and general health in a number of areas. A healthy oral cavity contributes to general wellbeing, but poor oral health can increase the risk of physical disorders, such as aspiration pneumonia, cardiovascular disorders, diabetes mellitus, and other disorders. Given the risks of these systemic diseases for frail older people, adequate and high-quality oral care is of great importance for this patient group.


Assuntos
Diabetes Mellitus , Pneumonia Aspirativa , Idoso , Idoso de 80 Anos ou mais , Idoso Fragilizado , Nível de Saúde , Humanos , Boca , Saúde Bucal
11.
Br Dent J ; 227(11): 975, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31844225
12.
Br Dent J ; 227(12): 1041, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31873260
13.
Wiad Lek ; 72(11 cz 1): 2089-2093, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31860852

RESUMO

OBJECTIVE: Introduction: To date, there are techniques that allow dental surgeons to restore the lost volume of bone mass, but the level of complications during augmentation remains consistently high. One of the main types of postoperative complications is the exposure of bone augmentate and its infection as a result of ischemic or destructive processes in the mucous shreds, which cover the augmentate, resulting from their overgrowth. The aim: The purpose of our research was to increase bone augmentation effectiveness in patients with secondary edentulism and to reduce risk of postoperative complications caused by ischemia of the mucous membranes because of their tension by mathematical simulation of tensile limits and permissible deformation for the mucous membranes of the oral cavity. PATIENTS AND METHODS: Materials and methods: As a research method was selected a two-component Mooney-Rivlin model, taking into account the indicators of elasticity and static strength of flat samples in tension, which allows them to perform hyperprime behavior at small and moderate deformations. For computer simulation of epithelial flap deformation during operation by finite element method we used ANSYS software environment. RESULTS: Results: Since elastic forces are potential, the work of forces does not depend on the way of tension. Only the initial and final states of the sample, i.e. its initial and final forms, play a role. CONCLUSION: Conclusions: The flap of 30 × 25 mm can be stretched and thus it is necessary to eliminate the deficit of fabric up to 5 mm wide in the direction of the Y axis (vertical axis). The relative elongation is λ = 25/20 = 1.25. The flap tension first occurs in the y direction.


Assuntos
Modelos Teóricos , Retalhos Cirúrgicos , Simulação por Computador , Elasticidade , Humanos , Boca
14.
Pol Merkur Lekarski ; 47(280): 123-127, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31760393

RESUMO

The possible connection between oral health and overall health has been a subject of debate among medical practitioners and theoreticians for millenia. AIM: The aim of the study was to determine the frequency of bacterial or fungal mouth infections in myocardial infarction patients treated with primary coronary intervention. MATERIALS AND METHODS: A total of 128 patients took part in the study (68 patients in the study group and 60 in the control group). The medical histories of the patients were taken, and the following tests were performed: a physical examination and an oral hygiene assessment. The oral hygiene assessment included a mycological evaluation of the oral cavity and bacteriological and mycological analyses of samples taken from gingival pockets. RESULTS: Fungal colonisation of the oral cavity was more prevalent in the study group than in the control group - 33 (50.0 %) vs 15 (25.0 %) p < 0.05. No statistically significant differences between the study and control groups were noted in terms of the number of positive bacteriological cultures. When it comes to predictors of myocardial infarction, fungal infections in the oral cavity were deemed to be a significant prognostic factor (OR 3.0). No similar correlation was identified in the case of bacterial infections. CONCLUSIONS: The study results suggest that poor oral health may be one of the predisposing factors for ischaemic heart disease, including in its acute form, i.e. myocardial infarction.


Assuntos
Micoses , Infarto do Miocárdio , Humanos , Boca , Micoses/complicações , Micoses/epidemiologia , Infarto do Miocárdio/complicações , Higiene Bucal , Prevalência
15.
Am J Orthod Dentofacial Orthop ; 156(5): 582-592, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31677666

RESUMO

INTRODUCTION: There is a high degree of uncertainty regarding the appropriate force level that should be applied during orthodontic tooth movement (OTM). As a result, orthodontic treatments may take longer than necessary, leading to unwanted side effects. This review aimed to identify an optimal force range with the rate of OTM as the primary outcome. External apical root resorption and pain were evaluated as secondary outcomes, and the influence of growth was examined. METHODS: Five electronic databases were searched (MEDLINE [via PubMed], Embase [via OVID], Cochrane Library, CINAHL, and Web of Science) with no publication date or language restrictions. Inclusion eligibility screening, quality assessment, and data extraction were performed by 3 investigators. Each retrieved record was assessed by 2 observers independently. Only randomized controlled trials and randomized split-mouth studies were included. RESULTS: A total of 12 articles satisfied the inclusion criteria-two randomized controlled trials and 10 randomized split-mouth studies. Only 1 study showed a low risk of bias, whereas the remaining 11 were unclear. The qualitative analysis showed that forces between 50 cN and 250 cN produced a similar OTM rate; forces >250 cN yielded a slightly higher rate but were accompanied by adverse effects. Because of considerable heterogeneity in methodology, clinical diversity with varying forces between 18 cN and 360 cN, and poor statistical reporting, a meta-analysis was deemed inappropriate. CONCLUSIONS: Forces between 50 cN and 100 cN seem optimal for OTM, patient comfort and potentially exhibit fewer side effects. Nevertheless, careful data interpretation is necessary because of the lack of strong evidence. Protocol registration: PROSPERO CRD42016039985.


Assuntos
Braquetes Ortodônticos , Técnicas de Movimentação Dentária , Humanos , Boca , Aparelhos Ortodônticos Fixos , Fenômenos Físicos , Ensaios Clínicos Controlados Aleatórios como Assunto
16.
Am J Orthod Dentofacial Orthop ; 156(5): 633-640, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31677672

RESUMO

INTRODUCTION: Although the Invisalign system has been used widely in recent years, the influences of this treatment on the oral microbiome and whether or not this influence is different from that of fixed appliances is still unknown. In this study, we investigated the changes in the oral microbiome in patients treated with the Invisalign system or with fixed appliances. METHODS: Fifteen subjects were enrolled, comprising 5 fixed appliance patients, 5 Invisalign patient, and 5 healthy controls. Saliva samples were collected, and high-throughput pyrosequencing was performed based on the 16S rRNA gene. RESULTS: Both fixed and Invisalign orthodontic treatments resulted in dysbiosis of the oral microbiome. Firmicutes and TM7 at the phyla level and Neisseria at the genus level displayed statistically significant differences between the 2 orthodontic groups. The effect of these changes with microbiome on oral health was inconsistent. The inferred microbial function of the Invisalign group suggested this group was more predisposed to periodontal diseases. CONCLUSION: The influence of the Invisalign system on the oral microbiome was no better for oral health compared with fixed appliances. The convenience of maintaining oral hygiene rather than changes in the oral microbiome may be the underlying reason for the performance of the Invisalign system on oral health.


Assuntos
Microbiota , Aparelhos Ortodônticos Fixos , Aparelhos Ortodônticos Removíveis , Humanos , Boca/microbiologia , Higiene Bucal , Aparelhos Ortodônticos , RNA Ribossômico 16S
17.
Oral Maxillofac Surg ; 23(4): 507-511, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31673818

RESUMO

INTRODUCTION: Povidone-iodine (PI) is thought to be an effective disinfectant and safe for many surgeons. Aspiration pneumonia is usually caused by gastric contents, but if PI solution will be aspirated, pneumonia or other complications may occur. CASE REPORT: We present a case of pneumonia to aspiration of PI solution in a 91-year-old man patient who underwent oral-maxillofacial surgery. When surgeons used PI solution for disinfection into the oral cavity, the solution seems to be sinking gradually. The patient showed severe respiratory distress and developed hypoxia. There were much frothy fluids into a tracheal tube. We suctioned through the endotracheal tube and performed bronchoscopy, that revealed a redness which appeared associated to a chemical injury on the left trachea and bronchus. His condition was complicated by ARDS and DIC. Periodical bronchial suction and guideline-based treatments of ARDS were carried in ICU. He recovered without severe complication. CONCLUSION: Although PI solution for an oral disinfection is used routinely, all operators need to be aware of the risk for PI aspiration.


Assuntos
Iodo , Pneumonia Aspirativa , Idoso de 80 Anos ou mais , Desinfecção , Humanos , Masculino , Boca , Povidona-Iodo
18.
BMC Evol Biol ; 19(1): 207, 2019 11 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703555

RESUMO

BACKGROUND: Parasite attachment structures are critical traits that influence effective host exploitation and survival. Morphology of attachment structures can reinforce host specificity and niche specialisation, or even enable host switching. Therefore, it is important to understand the determinants of variation in attachment structures. Cymothoid isopods are striking ectoparasites of fishes that include the infamous 'tongue-biters.' They are known to parasitise hosts in one of four qualitatively distinct anatomical regions. Here, we quantify variation in cymothoid attachment structures - hook-like appendages called dactyli - and test whether differences in dactylus shape are correlated with parasite mode (where they attach), allometry, or both, using multivariate ordinary least squares regression. We also assess the influence of shared ancestry on shape using a molecular phylogeny to weight our models using phylogenetic generalised least squares regression. RESULTS: We find clear differences in shape between externally-attaching and internally-attaching cymothoids but also between anterior and posterior dactyli across various species with the same attachment mode. Allometric effects are significant for anterior but not posterior dactyli. Mouth-attaching species show greater shape variability than gill- and mouth-attaching species. We find no evidence that there are clade-specific patterns of association between parasite mode and dactylus shape. CONCLUSIONS: Parasite mode appears to be the main driver of attachment morphology. This likely reflects several components of parasite ecology including feeding and functional demands of attachment in different microhabitats. Geometric morphometric approaches to the quantification of shape variation of simple structures is an effective tool that provides new insights into the evolvability of parasite attachment.


Assuntos
Ectoparasitoses/veterinária , Doenças dos Peixes/parasitologia , Interações Hospedeiro-Parasita , Isópodes/anatomia & histologia , Isópodes/fisiologia , Animais , Ectoparasitoses/parasitologia , Feminino , Peixes , Brânquias/parasitologia , Isópodes/classificação , Masculino , Boca/parasitologia , Filogenia
19.
BMC Complement Altern Med ; 19(1): 303, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703673

RESUMO

BACKGROUND: Candida albicans is an opportunistic pathogen that causes oral candidiasis and denture stomatitis. It has also been reported to infect oral mucositis lesions in patients who suffer from cancer affecting the head and neck and who receive chemotherapy and radiotherapy treatments. This study aimed to investigate the effects of two cinnamon bark fractions, i.e., an essential oil and an aqueous extract enriched in proanthocyanidins (Cinnulin PF®) on growth, biofilm formation, and adherence properties of C. albicans as well as on oral epithelial cells (barrier integrity, inflammatory response). METHODS: A microplate dilution assay was used to determine antifungal and anti-biofilm properties. A fluorescent assay was used to determine C. albicans adherence to oral epithelial cells. Cytotoxicity toward oral epithelial cells was assessed by determination of cell metabolic activity. Tight junction integrity of gingival keratinocytes was assessed by determination of transepithelial electrical resistance. IL-6 and IL-8 secretion by TNFα-stimulated oral epithelial cells was quantified by ELISA. RESULTS: While Cinnulin PF® did not reduce C. albicans growth, the cinnamon bark oil exhibited high antifungal activity with minimum inhibitory concentrations and minimum fungicidal concentrations in the range of 0.039 to 0.078%. The cinnamon oil was also active against a pre-formed C. albicans biofilm. Interestingly, Cinnulin PF® prevented biofilm formation by C. albicans and attenuated its adherence to oral epithelial cells. At their effective concentrations, the cinnamon oil and the Cinnulin PF® displayed no significant cytotoxicity against oral epithelial cells. In an in vitro model, both cinnamon fractions reinforced the integrity of the oral epithelial barrier. Lastly, Cinnulin PF® inhibited the secretion of interleukin-6 and interleukin-8 by oral epithelial cells stimulated with TNF-α. CONCLUSION: By their ability to attenuate growth, biofilm formation and adherence property of C. albicans, to reinforce the epithelial barrier function, and to exert anti-inflammatory properties the two cinnamon fractions (essential oil, Cinnulin PF®) investigated in the present study may be promising agents for treating oral infections involving C. albicans.


Assuntos
Antifúngicos/farmacologia , Candida albicans/efeitos dos fármacos , Candidíase Bucal/microbiologia , Cinnamomum zeylanicum/química , Células Epiteliais/microbiologia , Boca/microbiologia , Óleos Voláteis/farmacologia , Biofilmes/efeitos dos fármacos , Candida albicans/crescimento & desenvolvimento , Candida albicans/fisiologia , Candidíase Bucal/tratamento farmacológico , Linhagem Celular , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Humanos , Interleucina-6/metabolismo , Interleucina-8/metabolismo , Boca/metabolismo , Casca de Planta/química
20.
Rev Med Suisse ; 15(672): 2131-2136, 2019 Nov 20.
Artigo em Francês | MEDLINE | ID: mdl-31746569

RESUMO

Many diseases affect the oral cavity. Therefore, the mouth is an organ that internists should observe carefully. Hereafter we present five illustrations of oral semiology that reflect a systemic condition: a strawberry tongue, an ulceration, a labial lesion, a gingival hyperplasia and a gingival hyperpigmentation. Each time, a differential diagnosis is to be considered, potentially allowing the identification of severe diseases.


Assuntos
Medicina Interna/métodos , Doenças da Boca/complicações , Doenças da Boca/diagnóstico , Boca/patologia , Diagnóstico Diferencial , Humanos , Doenças da Boca/patologia , Mucosa Bucal/patologia
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