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1.
Sci Rep ; 14(1): 12988, 2024 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-38844823

RESUMO

Salinity stress significantly hinders plant growth by disrupting osmotic balance and inhibiting nutrient uptake, leading to reduced biomass and stunted development. Using saponin (SAP) and boron (B) can effectively overcome this issue. Boron decreases salinity stress by stabilizing cell walls and membranes, regulating ion balance, activating antioxidant enzymes, and enhancing water uptake. SAP are bioactive compounds that have the potential to alleviate salinity stress by improving nutrient uptake, modulating plant hormone levels, promoting root growth, and stimulating antioxidant activity. That's why the current study was planned to use a combination of SAP and boron as amendments to mitigate salinity stress in sweet potatoes. Four levels of SAP (0%, 0.1%, 0.15%, and 0.20%) and B (control, 5, 10, and 20 mg/L B) were applied in 4 replications following a completely randomized design. Results illustrated that 0.15% SAP with 20 mg/L B caused significant enhancement in sweet potato vine length (13.12%), vine weight (12.86%), root weight (8.31%), over control under salinity stress. A significant improvement in sweet potato chlorophyll a (9.84%), chlorophyll b (20.20%), total chlorophyll (13.94%), photosynthetic rate (17.69%), transpiration rate (16.03%), and stomatal conductance (17.59%) contrast to control under salinity stress prove the effectiveness of 0.15% SAP + 20 mg/L B treatment. In conclusion, 0.15% SAP + 20 mg/L B is recommended to mitigate salinity stress in sweet potatoes.


Assuntos
Boro , Ipomoea batatas , Estresse Salino , Saponinas , Ipomoea batatas/crescimento & desenvolvimento , Boro/farmacologia , Saponinas/farmacologia , Estresse Salino/efeitos dos fármacos , Fotossíntese/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/efeitos dos fármacos , Clorofila/metabolismo , Sinergismo Farmacológico , Salinidade
2.
BMC Plant Biol ; 24(1): 550, 2024 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-38872083

RESUMO

BACKGROUND: Boron (B) is a micronutrient, but excessive levels can cause phytotoxicity, impaired growth, and reduced photosynthesis. B toxicity arises from over-fertilization, high soil B levels, or irrigation with B-rich water. Conversely, silicon (Si) is recognized as an element that mitigates stress and alleviates the toxic effects of certain nutrients. In this study, to evaluate the effect of different concentrations of Si on maize under boron stress conditions, a factorial experiment based on a randomized complete block design was conducted with three replications in a hydroponic system. The experiment utilized a nutrient solution for maize var. Merit that contained three different boron (B) concentrations (0.5, 2, and 4 mg L-1) and three Si concentrations (0, 28, and 56 mg L-1). RESULTS: Our findings unveiled that exogenous application of B resulted in a substantial escalation of B concentration in maize leaves. Furthermore, B exposure elicited a significant diminution in fresh and dry plant biomass, chlorophyll index, chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoids, and membrane stability index (MSI). As the B concentration augmented, malondialdehyde (MDA) content and catalase (CAT) enzyme activity exhibited a concomitant increment. Conversely, the supplementation of Si facilitated an amelioration in plant fresh and dry weight, total carbohydrate, and total soluble protein. Moreover, the elevated activity of antioxidant enzymes culminated in a decrement in hydrogen peroxide (H2O2) and MDA content. In addition, the combined influence of Si and B had a statistically significant impact on the leaf chlorophyll index, total chlorophyll (a + b) content, Si and B accumulation levels, as well as the enzymatic activities of guaiacol peroxidase (GPX), ascorbate peroxidase (APX), and H2O2 levels. These unique findings indicated the detrimental impact of B toxicity on various physiological and biochemical attributes of maize, while highlighting the potential of Si supplementation in mitigating the deleterious effects through modulation of antioxidant machinery and biomolecule synthesis. CONCLUSIONS: This study highlights the potential of Si supplementation in alleviating the deleterious effects of B toxicity in maize. Increased Si consumption mitigated chlorophyll degradation under B toxicity, but it also caused a significant reduction in the concentrations of essential micronutrients iron (Fe), copper (Cu), and zinc (Zn). While Si supplementation shows promise in counteracting B toxicity, the observed decrease in Fe, Cu, and Zn concentrations warrants further investigation to optimize this approach and maintain overall plant nutritional status.


Assuntos
Boro , Clorofila , Hidroponia , Silício , Zea mays , Zea mays/efeitos dos fármacos , Zea mays/crescimento & desenvolvimento , Zea mays/fisiologia , Zea mays/metabolismo , Boro/toxicidade , Boro/metabolismo , Silício/farmacologia , Clorofila/metabolismo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/crescimento & desenvolvimento , Malondialdeído/metabolismo , Carotenoides/metabolismo , Antioxidantes/metabolismo , Catalase/metabolismo
3.
Zhongguo Yi Liao Qi Xie Za Zhi ; 48(3): 271-276, 2024 May 30.
Artigo em Chinês | MEDLINE | ID: mdl-38863092

RESUMO

In order to improve the biological effect of proton therapy, the authors first propose a new method of boron-based proton-enhanced radiotherapy in a " ternary " radiotherapy mode, based on the existing sensitizing effect of proton radiotherapy: i.e, Boron-based mediators (11B and 10B) induce the proton-hydrogen-boron fusion reaction of the low-energy protons arriving at the Bragg peak region of the tumor target area (p+11B→3α) and thermal neutron capture (10B+n→7Li3+(0.84 MeV)+4He2+(1.47 MeV)+γ(0.477 MeV)), which release low-energy α-particles with high LETs to enhance the biological effect of proton dose in the target area, thus improve the clinical effect of proton therapy. Then, the advantages and disadvantages of the "ternary" model were analyzed from the theoretical basis and current research status, and finally, the "ternary" model is summarized and prospected.


Assuntos
Terapia com Prótons , Prótons , Boro , Neoplasias/radioterapia , Dosagem Radioterapêutica , Partículas alfa/uso terapêutico , Modelos Teóricos
4.
Biomaterials ; 309: 122605, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38754291

RESUMO

Multidisciplinary therapy centered on radical surgery for resectable pancreatic cancer is expected to prolong prognosis, but relies on CA19-9 biomarker levels to determine treatment strategy. Boron neutron capture therapy (BNCT) is a chemoradiotherapy using tumor hyperaccumulator boron drugs and neutron irradiation. The purpose of this study is to investigate novel boron drug agents for BNCT for pancreatic cancer. Bioinformatics was used to evaluate the uptake of current boron amino acid (BPA) drugs for BNCT into pancreatic cancer. The expression of the amino acid transporter LAT1, a BPA uptake transporter, was low in pancreatic cancer and even lower in high CA19-9 pancreatic cancer. In contrast, the glucose transporter was high in high CA19-9 pancreatic cancers and inversely correlated with LAT1 expression. Considering the low EPR effect in pancreatic cancer, we synthesized a small molecule Glucose-BSH, which is boron BSH bound to glucose, and confirmed its specific uptake in pancreatic cancer. uptake of Glucose-BSH was confirmed in an environment compatible with the tumor microenvironment. The therapeutic efficacy and safety of Glucose-BSH by therapeutic neutron irradiation were confirmed with BNCT. We report Glucose-BSH boron drug discovery study of a Precision Medicine BNCT with application to high CA19-9 pancreatic cancer.


Assuntos
Terapia por Captura de Nêutron de Boro , Glucose , Neoplasias Pancreáticas , Terapia por Captura de Nêutron de Boro/métodos , Neoplasias Pancreáticas/terapia , Neoplasias Pancreáticas/patologia , Humanos , Glucose/metabolismo , Linhagem Celular Tumoral , Animais , Compostos de Boro/química , Compostos de Boro/uso terapêutico , Boro/química , Feminino , Camundongos Nus
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 318: 124470, 2024 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-38761476

RESUMO

Recently, nanomaterials have attracted a lot of attention due to their potential as effective fluorescent nano-sensor probes. They were distinguishing substitutes for other luminescent techniques, such as fluorescent dyes and luminous derivatization, because of their affordability, environmental friendliness, and special photocatalytic properties. In the suggested work, a straightforward method was used to create boron and nitrogen carbon dots (B@CDs) with a good quantum yield value of 31.15 % utilizing boric acid and di-sodium EDTA. For the purpose of characterizing QDs, a variety of instruments were employed, such as transmission electron microscopy, fluorescence spectroscopy, X-ray FTIR, and UV-VIS spectroscopy. Nebivolol (NEB) is a cardiovascular medication used globally to treat congestive heart failure and hypertension, is in the meantime. For this reason, a brand-new, environmentally friendly analytical technique was created to determine the amount of human plasma, uniformity test, and commercial nebivolol (NEB) tablets. After gradually adding NEB, the response of B@CQDs was enhanced at 438 nm (excitation at 371 nm). The calibration graph ranged between 20 and 500 ng mL-1 with a quantification limit (LOQ) of 2.50 ng mL-1 and a detection limit (LOD) of 0.82 ng mL-1.


Assuntos
Boro , Carbono , Nebivolol , Pontos Quânticos , Nebivolol/sangue , Nebivolol/análise , Humanos , Carbono/química , Pontos Quânticos/química , Boro/química , Química Verde/métodos , Espectrometria de Fluorescência/métodos , Limite de Detecção , Espectroscopia de Infravermelho com Transformada de Fourier , Comprimidos , Espectrofotometria Ultravioleta
6.
J Environ Manage ; 359: 121084, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38723505

RESUMO

Extensive global dependency on rice and wheat crops has necessitated the adoption of intensive cultivation practices, thereby compelling to closely monitor the potential yield-limiting factors, among which, boron (B) deficiency stands out to be a prime concern. The present study explores the effects of B fertilization strategies within the Rice-Wheat Cropping System (RWCS) in the Tarai region of North-West India. A comprehensive six-year field experiment was conducted (2013-2019) at G.B. Pant University of Agriculture and Technology, Uttarakhand, India. The experiment tested graded B doses (0.5, 1.0, 1.5, and 2.0 kg ha-1) at varied frequencies (single, alternate, and annual) in a factorial design. The study revealed significant impacts of alternate B application at 1.5 kg ha-1 on crop yields and the Sustainable Yield Index (SYI). The System Rice Equivalent Yield (SREY) exhibited an increase of 6.7% with B supplementation over B-deprived plots, highlighting the pivotal role of B fertilizer in enhancing productivity within the RWCS. The economic optimum B dose was found to be 1.422 kg ha-1 using a linear plus plateau model, resulting in a calculated annual SREY of 9.73 t ha-1 when applied alternately to the cropping system. Continuous application and higher B rates demonstrated substantial increases in various B fractions, while the mobility factor remained within 10%, depicting safe ecological limits. The distribution of fractions in B-treated plots on average followed the order: residual B > organically-bound B > oxide bound B > specifically adsorbed B > readily soluble B. Similarities in the distribution patterns of B fractions between B-treated plots and the control indicated potential influence of biotic or abiotic processes on B fraction dynamics, even in the absence of external B application. To sum up, B application in alternate years at 1.5 kg ha-1 was most sustainable in enhancing the SREY, SYI, available soil B, and B fractions and lowering the environmental hazards.


Assuntos
Agricultura , Boro , Produtos Agrícolas , Fertilizantes , Oryza , Triticum , Oryza/crescimento & desenvolvimento , Triticum/crescimento & desenvolvimento , Índia , Agricultura/métodos , Produtos Agrícolas/crescimento & desenvolvimento , Solo/química
7.
Anal Chim Acta ; 1311: 342715, 2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38816154

RESUMO

BACKGROUND: Due to that the higher activity of nanozymes would bring outstanding performance for the nanozyme-based biosensing strategies, great efforts have been made by researchers to improve the catalytic activity of nanozymes, and novel nanozymes with high catalytic activity are desired. Considering the crucial role in controlling blood glucose level, strategies like colorimetric and chemiluminescence to monitor α-glucosidase are developed. However, multi-mode detection with higher sensitivity was insufficient. Therefore, developing triple-mode detection method for α-glucosidase based on great performance nanozyme is of great importance. RESULTS: In this work, a novel nanozyme Cu-BCN was synthesized by loading Cu on boron doped carbon substrate g-C3N4 and applied to the colorimetric-fluorescent-smartphone triple-mode detection of α-glucosidase. In the presence of H2O2, Cu-BCN catalyzed the generation of 1O2 from H2O2, 1O2 subsequently oxidized TMB to blue colored oxTMB. In the presence of hydroquinone (HQ), the ROS produced from H2O2 was consumed, inhibiting the oxidation of TMB, which endows the possibility of colorimetric and visual on-site detection of HQ. Further, due to that the fluorescence of Mg-CQDs at 444 nm could be quenched by oxTMB, HQ could also be quantified through fluorescent mode. Since α-glucosidase could efficiently hydrolyze α-arbutin into HQ, the sensitive detection of α-glucosidase was realized. Further, colorimetric paper-based device (c-PAD) was fabricated for on-site α-glucosidase detection. The LODs for α-glucosidase via three modes were 2.20, 1.62 and 2.83 U/L respectively, high sensitivities were realized. SIGNIFICANCE: The nanozyme Cu-BCN possesses higher peroxidase-like activity by doping boron to the substrate than non-doped Cu-CN. The proposed triple-mode detection of α-glucosidase is more sensitive than most previous reports, and is reliable when applied to practical sample. Further, the smartphone-based colorimetric paper-based analytical device (c-PAD) made of simple materials could also detect α-glucosidase sensitively. The smartphone-based on-site detection provided a convenient, instrument-free and sensitive sensing method for α-glucosidase.


Assuntos
Boro , Colorimetria , Cobre , Smartphone , alfa-Glucosidases , Colorimetria/métodos , Cobre/química , alfa-Glucosidases/metabolismo , alfa-Glucosidases/química , Boro/química , Compostos de Nitrogênio/química , Limite de Detecção , Técnicas Biossensoriais/métodos , Corantes Fluorescentes/química , Humanos , Grafite
8.
Chemosphere ; 359: 142334, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38759811

RESUMO

This study presents a baseline evaluation of the distribution, human and ecotoxicological risk, and the potential interactions of fluoride and boron in the water-sediment interface in 25 locations from incredible Red Sea tourist destinations. Results showed comparable levels of B and F in the water and sediments with previous literature. Significant positive correlation was found between B and F (r = 0.57; P<0.01). Based on the sediment/liquid partition coefficient (Kd), F is more likely to be released from the sediment into seawater (logKd< 3) than B (3< logKd< 4). pH and alkalinity may affect water-sediment interactions of B and F, respectively, while SO42- and Cl- ions had no significant effect on adsorption ability of F and B. The majority of minerals had average saturation Index (SI) > 1 referring to the over saturation of seawater with these minerals and their inability to dissolve. The formation of CF, FAP, and CFAP may be related to the high correlation between Fw (r = 0.928, P< 0.01; r = 0.527, P< 0.01; r = 0.608, P< 0.01) and Bw (r = 0.38, P< 0.05; r = 0.38, P< 0.05; r = 0.397, P< 0.05). Total hazard quotient (THQ) for children and adults were <1, revealing no health risks from exposure to B and F through ingestion and skin contact while swimming. The risk characterization ratio; RCRmix(MEC/PNEC) showed high short-term risks to aquatic organisms. Further investigations might emphasis on emerging mitigation strategies to address these concerns.


Assuntos
Boro , Monitoramento Ambiental , Fluoretos , Sedimentos Geológicos , Água do Mar , Poluentes Químicos da Água , Boro/análise , Boro/química , Poluentes Químicos da Água/análise , Medição de Risco , Egito , Água do Mar/química , Fluoretos/análise , Sedimentos Geológicos/química , Humanos , Oceano Índico , Íons , Adulto
9.
Bioresour Technol ; 403: 130883, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38788807

RESUMO

Electrodes with superior stability and sensitivity are highly desirable in advancing the toxicity detection efficiency of microbial fuel cells (MFCs). Herein, boron-doped reduced graphene oxide (B-rGO) was synthesized and utilized as an efficient cathode candidate in an MFCs system for sensitive sodium dodecylbenzene sulfonate (SDBS) detection. Boron doping introduces additional defects and improves the dispersibility and oxygen permeability, thereby enhancing the oxygen reduction reaction (ORR) efficiency. The B-rGO-based cathode has demonstrated significantly improved output voltage and power density, marking improvements of 75 % and 58 % over their undoped counterparts, respectively. Furthermore, it also exhibited remarkable linear sensitivity to SDBS concentrations across a broad range (0.2-15 mg/L). Notably, the cathode maintained excellent stability within the test range and showed significant reversibility for SDBS concentrations between 0.2 and 3 mg/L. The highly sensitive and stable B-rGO-based cathode is inspiring for developing more practical and cost-effective toxicant sensing devices.


Assuntos
Fontes de Energia Bioelétrica , Boro , Eletrodos , Grafite , Grafite/química , Boro/química , Benzenossulfonatos/química , Oxirredução , Óxidos/química
10.
ACS Appl Mater Interfaces ; 16(20): 26537-26546, 2024 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-38739859

RESUMO

Water-stable organic radicals are promising photothermal conversion candidates for photothermal therapy (PTT). However, organic radicals are usually unstable in biological environments, which greatly hinders their wide application. Here, we have developed a chaotropic effect-based and photoinduced water-stable supramolecular radical (MB12-2) for efficient antibacterial PTT. The supramolecular radical precursor MB12-1 was constructed by the chaotropic effect between closo-dodecaborate cluster (B12H122-) and N,N'-dimethylated dipyridinium thiazolo [5,4-d] thiazole (MPT2+). Subsequently, with triethanolamine (TEOA) serving as an electron donor, MB12-1 could transform to its radical form MB12-2 through photoinduced electron transfer (PET) under 435-nm laser irradiation. The N2 adsorption-desorption analysis confirmed that MB12-2 was tightly packed through the introduction of B12H122-, which effectively enhanced its stability via a spatial site-blocked effect. Moreover, the half-life of MB12-2 in water was calculated through ultraviolet-visible light (UV-vis) absorption spectra results for periods as long as 20 days. In addition, in the skin infection model, MB12-2, as a wound dressing, showed remarkable photothermal antibacterial activity (>97%) under 660-nm laser irradiation and promoted wound healing. This study presents a simple method for designing long-term water-stable supramolecular radicals, offering a novel avenue for noncontact treatments for bacterial infections.


Assuntos
Antibacterianos , Terapia Fototérmica , Antibacterianos/química , Antibacterianos/farmacologia , Animais , Água/química , Camundongos , Radicais Livres/química , Boro/química , Boro/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos
11.
Int J Mol Sci ; 25(9)2024 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-38732218

RESUMO

Boronate esters are a class of compounds containing a boron atom bonded to two oxygen atoms in an ester group, often being used as precursors in the synthesis of other materials. The characterization of the structure and properties of esters is usually carried out by UV-visible, infrared, and nuclear magnetic resonance (NMR) spectroscopic techniques. With the aim to better understand our experimental data, in this article, the density functional theory (DFT) is used to analyze the UV-visible and infrared spectra, as well as the isotropic shielding and chemical shifts of the hydrogen atoms 1H, carbon 13C and boron 11B in the compound 4-(4,4,5,5-tetramethyl-1,3,2-dioxoborolan-2-yl)benzaldehyde. Furthermore, this study considers the change in its electronic and spectroscopic properties of this particular ester, when its boron atom is coordinated with a fluoride anion. The calculations were carried out using the LSDA and B3LYP functionals in Gaussian-16, and PBE in CASTEP. The results show that the B3LYP functional gives the best approximation to the experimental data. The formation of a coordinated covalent B-F bond highlights the remarkable sensitivity of the NMR chemical shifts of carbon, oxygen, and boron atoms and their surroundings. Furthermore, this bond also highlights the changes in the electron transitions bands n → π* and π → π* during the absorption and emission of a photon in the UV-vis, and in the stretching bands of the C=C bonds, and bending of BO2 in the infrared spectrum. This study not only contributes to the understanding of the properties of boronate esters but also provides important information on the interactions and responses optoelectronic of the compound when is bonded to a fluorine atom.


Assuntos
Benzaldeídos , Benzaldeídos/química , Espectroscopia de Ressonância Magnética , Teoria da Densidade Funcional , Flúor/química , Boro/química , Modelos Moleculares , Ésteres/química , Espectrofotometria Infravermelho , Estrutura Molecular , Íons/química
12.
PLoS One ; 19(5): e0303570, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38781202

RESUMO

Boron cluster sheets are two-dimensional boron atom-based formations called borophene. They are similar to the two-dimensional sheet known as graphene, which is composed of carbon atoms arranged in a hexagonal lattice. The unique electrical, mechanical, and thermal properties of borophene make it a sought-after substance for a variety of uses, such as catalysis, energy storage, and electronics. There are two ways to manufacture borophene: chemical vapor deposition and molecular beam epitaxy. Vertex-edge valency-based topological descriptors are a great example of a molecular descriptor that provides information on the connection of atoms in a molecule. These descriptions are based on the notion that a node's value in a molecular network is the sum of the valency of those atoms that are directly connected to that node. In this article, we discussed some novel vertex-edge (ve) and edge-vertex (ev) topological descriptors and found their formulations for the boron cluster or borophene sheets. Also, we show the numerical and graphical comparison of these descriptors in this article.


Assuntos
Boro , Boro/química , Compostos de Boro/química , Grafite/química , Modelos Moleculares , Estrutura Molecular
13.
Physiol Plant ; 176(3): e14358, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38783511

RESUMO

Soil salinity is a major environmental constraint affecting the sustainability and profitability of agricultural production systems. Salinity stress tolerance has been present in wild crop relatives but then lost, or significantly weakened, during their domestication. Given the genetic and physiological complexity of salinity tolerance traits, agronomical solutions may be a suitable alternative to crop breeding for improved salinity stress tolerance. One of them is optimizing fertilization practices to assist plants in dealing with elevated salt levels in the soil. In this review, we analyse the causal relationship between the availability of boron (an essential metalloid micronutrient) and plant's adaptive responses to salinity stress at the whole-plant, cellular, and molecular levels, and a possibility of using boron for salt stress mitigation. The topics covered include the impact of salinity and the role of boron in cell wall remodelling, plasma membrane integrity, hormonal signalling, and operation of various membrane transporters mediating plant ionic and water homeostasis. Of specific interest is the role of boron in the regulation of H+-ATPase activity whose operation is essential for the control of a broad range of voltage-gated ion channels. The complex relationship between boron availability and expression patterns and the operation of aquaporins is also discussed.


Assuntos
Boro , Salinidade , Solo , Boro/metabolismo , Solo/química , Adaptação Fisiológica/genética , Tolerância ao Sal/genética , Plantas/metabolismo , Plantas/genética , Regulação da Expressão Gênica de Plantas
14.
J Hazard Mater ; 472: 134458, 2024 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-38703679

RESUMO

Diclofenac (DCF) is an environmentally persistent, nonsteroidal anti-inflammatory drug (NSAID) with thyroid disrupting properties. Electrochemical advanced oxidation processes (eAOPs) can efficiently remove NSAIDs from wastewater. However, eAOPs can generate transformation products (TPs) with unknown chemical and biological characteristics. In this study, DCF was electrochemically degraded using a boron-doped diamond anode. Ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry was used to analyze the TPs of DCF and elucidate its potential degradation pathways. The biological impact of DCF and its TPs was evaluated using the Xenopus Eleutheroembryo Thyroid Assay, employing a transgenic amphibian model to assess thyroid axis activity. As DCF degradation progressed, in vivo thyroid activity transitioned from anti-thyroid in non-treated samples to pro-thyroid in intermediately treated samples, implying the emergence of thyroid-active TPs with distinct modes of action compared to DCF. Molecular docking analysis revealed that certain TPs bind to the thyroid receptor, potentially triggering thyroid hormone-like responses. Moreover, acute toxicity occurred in intermediately degraded samples, indicating the generation of TPs exhibiting higher toxicity than DCF. Both acute toxicity and thyroid effects were mitigated with a prolonged degradation time. This study highlights the importance of integrating in vivo bioassays in the environmental risk assessment of novel degradation processes.


Assuntos
Anti-Inflamatórios não Esteroides , Diclofenaco , Glândula Tireoide , Poluentes Químicos da Água , Animais , Diclofenaco/toxicidade , Diclofenaco/química , Diclofenaco/metabolismo , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/química , Glândula Tireoide/efeitos dos fármacos , Glândula Tireoide/metabolismo , Anti-Inflamatórios não Esteroides/toxicidade , Anti-Inflamatórios não Esteroides/química , Medição de Risco , Técnicas Eletroquímicas , Simulação de Acoplamento Molecular , Disruptores Endócrinos/toxicidade , Disruptores Endócrinos/química , Disruptores Endócrinos/metabolismo , Xenopus laevis , Diamante/química , Oxirredução , Boro/toxicidade , Boro/química
15.
Appl Radiat Isot ; 209: 111330, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38657372

RESUMO

Boron neutron capture therapy (BNCT) has received extensive attention as an advanced binary radiotherapy method. However, BNCT still faces poor selectivity of boron agent and is insufficient boron content in tumor tissues. To improve the tumor-targeted ability and boron content, this research aims to design, synthesize and preliminary evaluate a new borane agent Carborane-FAPI, which coupling the o-carborane to the compound skeleton of a mature fibroblast activating protein (FAP) inhibitor (FAPI). FAP is a tumor-associated antigen. FAP expressed lowly in normal organs and highly expressed in tumors, so it is a potential target for diagnosis and treatment. Boronophenylalanine (BPA) is the most widely investigated BNCT drug in present. Compared with BPA, the boron content of a single molecule is increased and drug targeting is enhanced. The results show that Carboaren-FAPI has low toxicity to normal cells, and selective enrichment in tumor tissues. It is a promising boron drug that has the potential to be used in BNCT.


Assuntos
Boranos , Terapia por Captura de Nêutron de Boro , Boro , Terapia por Captura de Nêutron de Boro/métodos , Humanos , Animais , Camundongos , Proteínas de Membrana/metabolismo , Endopeptidases , Serina Endopeptidases/metabolismo , Gelatinases/metabolismo , Compostos de Boro/uso terapêutico , Compostos de Boro/farmacocinética , Linhagem Celular Tumoral
16.
Inorg Chem ; 63(15): 6822-6835, 2024 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-38560761

RESUMO

Boron-dipyrromethene (BODIPY) dyes are promising photosensitizers for cellular imaging and photodynamic therapy (PDT) owing to their excellent photophysical properties and the synthetically tunable core. Metalation provides a convenient way to overcome the drawbacks arising from their low aqueous solubility. New photo-/redox-responsive Co(III) prodrug chaperones are developed as anticancer PDT agents for efficient cellular delivery of red-light-active BODIPY dyes. The photobiological activity of heteroleptic Co(III) complexes derived from tris(2-pyridylmethyl)amine (TPA) and acetylacetone-conjugated PEGylated distyryl BODIPY (HL1) or its dibromo analogue (HL2), [CoIII(TPA)(L1/L2)](ClO4)2 (1 and 2), are investigated. The Co(III)/Co(II) redox potential is tuned using the Co(III)-TPA scaffold. Complex 1 displays the in vitro release of BODIPY on red light irradiation. Complex 2, having good singlet oxygen quantum yield (ΦΔ âˆ¼ 0.28 in DMSO), demonstrates submicromolar photocytotoxicity to HeLa cancer cells (IC50 ≈ 0.23 µM) while being less toxic to HPL1D normal cells in red light. Cellular imaging using the emissive complex 1 shows mitochondrial localization and significant penetration into the HeLa tumor spheroids. Complex 2 shows supercoiled DNA photocleavage activity and apoptotic cell death through phototriggered generation of reactive oxygen species. The Co(III)-BODIPY prodrug conjugates exemplify new type of phototherapeutic agents with better efficacy than the organic dyes alone in the phototherapeutic window.


Assuntos
Antineoplásicos , Fotoquimioterapia , Porfobilinogênio/análogos & derivados , Pró-Fármacos , Humanos , Boro/farmacologia , Luz Vermelha , Corantes , Pró-Fármacos/farmacologia , Cobalto/farmacologia , Fármacos Fotossensibilizantes/efeitos da radiação , Antineoplásicos/efeitos da radiação , Compostos de Boro/farmacologia , Compostos de Boro/efeitos da radiação , Oxigênio Singlete/metabolismo , Luz
17.
J Nanobiotechnology ; 22(1): 183, 2024 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-38622691

RESUMO

BACKGROUND: The use of cells as carriers for the delivery of nanoparticles is a promising approach in anticancer therapy, mainly due to their natural properties, such as biocompatibility and non-immunogenicity. Cellular carriers prevent the rapid degradation of nanoparticles, improve their distribution, reduce cytotoxicity and ensure selective delivery to the tumor microenvironment. Therefore, we propose the use of phagocytic cells as boron carbide nanoparticle carriers for boron delivery to the tumor microenvironment in boron neutron capture therapy. RESULTS: Macrophages originating from cell lines and bone marrow showed a greater ability to interact with boron carbide (B4C) than dendritic cells, especially the preparation containing larger nanoparticles (B4C 2). Consequently, B4C 2 caused greater toxicity and induced the secretion of pro-inflammatory cytokines by these cells. However, migration assays demonstrated that macrophages loaded with B4C 1 migrated more efficiently than with B4C 2. Therefore, smaller nanoparticles (B4C 1) with lower toxicity but similar ability to activate macrophages proved to be more attractive. CONCLUSIONS: Macrophages could be promising cellular carriers for boron carbide nanoparticle delivery, especially B4C 1 to the tumor microenvironment and thus prospective use in boron neutron capture therapy.


Assuntos
Terapia por Captura de Nêutron de Boro , Nanopartículas , Boro , Linhagem Celular Tumoral , Nanopartículas/metabolismo , Macrófagos
18.
Sci Rep ; 14(1): 8265, 2024 04 09.
Artigo em Inglês | MEDLINE | ID: mdl-38594281

RESUMO

Boron neutron capture therapy (BNCT) is a type of targeted particle radiation therapy with potential applications at the cellular level. Spinal cord gliomas (SCGs) present a substantial challenge owing to their poor prognosis and the lack of effective postoperative treatments. This study evaluated the efficacy of BNCT in a rat SCGs model employing the Basso, Beattie, and Bresnahan (BBB) scale to assess postoperative locomotor activity. We confirmed the presence of adequate in vitro boron concentrations in F98 rat glioma and 9L rat gliosarcoma cells exposed to boronophenylalanine (BPA) and in vivo tumor boron concentration 2.5 h after intravenous BPA administration. In vivo neutron irradiation significantly enhanced survival in the BNCT group when compared with that in the untreated group, with a minimal BBB scale reduction in all sham-operated groups. These findings highlight the potential of BNCT as a promising treatment option for SCGs.


Assuntos
Terapia por Captura de Nêutron de Boro , Neoplasias Encefálicas , Glioma , Neoplasias da Medula Espinal , Ratos , Animais , Neoplasias Encefálicas/patologia , Ratos Endogâmicos F344 , Boro , Pesquisa Translacional Biomédica , Compostos de Boro/farmacologia , Glioma/patologia
19.
Food Chem ; 449: 139264, 2024 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-38593724

RESUMO

In this study, a microelectrode array sensor based on boron and nitrogen co-doped vertical graphene (BNVG) was assembled to quantify salicylic acid (SA) in living plants. The influence of B and N contents on the electrochemical reaction kinetics and SA response signal was investigated. A microneedle sensor with three optimized BNVG microelectrodes (3.57 at.% B and 3.27 at.% N) was used to quantitatively analyze SA in the 0.5-100 µM concentration range and pH 4.0-9.0, with limits of detection of 0.14-0.18 µM. Additionally, a quantitative electrochemical model database based on the BNVG microelectrode sensor was constructed to monitor the growth of cucumbers and cauliflowers, which confirmed that the SA level and plant growth rate were positively correlated. Moreover, the SA levels in various vegetables and fruits purchased from the market were measured to demonstrate the practical application prospects for on-site inspection and evaluation.


Assuntos
Boro , Técnicas Eletroquímicas , Frutas , Grafite , Microeletrodos , Nitrogênio , Ácido Salicílico , Verduras , Grafite/química , Ácido Salicílico/análise , Verduras/química , Frutas/química , Técnicas Eletroquímicas/instrumentação , Boro/química , Nitrogênio/análise , Agulhas , Cucumis sativus/química , Técnicas Biossensoriais/instrumentação , Limite de Detecção
20.
Bull Environ Contam Toxicol ; 112(5): 71, 2024 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-38684523

RESUMO

The remarkable optical properties and strong biocompatibility of carbon dots make them highly promising for applications in biochemical sensing and environmental testing. These carbon dots possess a surface that is easily modifiable. In this study, carbon dots have been successfully synthesized and modified by the addition of N and B dopants using the microwave method, along with the functionalization of their surface functional groups with bovine serum albumin (BSA). The maximum fluorescence intensity of N, B-CDs is observed at 462 nm when excited at a wavelength of 352 nm. N, B-CDs have a spherical size with a diameter ranging from 2 to 6 nm, confirmed by UV-Vis absorption spectra and the presence of functional groups in the FT-IR absorption patterns. BSA-functionalized N, B-CDs as the fluorescent probe demonstrate great potential as a sensor for Pb(II) ions in water, with a very low detection limit of 1.05 µg/L. This research could contribute to the development of fluorescence nanosensors.


Assuntos
Boro , Carbono , Chumbo , Nitrogênio , Pontos Quânticos , Chumbo/análise , Chumbo/química , Boro/química , Carbono/química , Nitrogênio/química , Nitrogênio/análise , Pontos Quânticos/química , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/química , Soroalbumina Bovina/química , Monitoramento Ambiental/métodos , Espectrometria de Fluorescência , Corantes Fluorescentes/química
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