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2.
Vet Parasitol ; 277: 108992, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31835054

RESUMO

Eprinomectin (EPM) has been recently granted a marketing authorisation in the European Union for use in goats, with a zero-day milk withdrawal period. Considering the high prevalence of benzimidazole resistance worldwide and the economic implications of managing milk residues, EPM may today be considered the main (or even the only) affordable treatment option, at least in dairy goats in the EU. However, the chosen dose (1 mg/kg) seems to be suboptimal, especially for lactating goats, and the chosen route of administration (Pour-on) highly subject to inter-individual variability. Considering the scarcity of anthelmintic resources, such a dosage regimen might threat the sustainability of this crucial drug in goat milk production and needs to be urgently discussed and reassessed.


Assuntos
Indústria de Laticínios/normas , Doenças das Cabras/tratamento farmacológico , Ivermectina/análogos & derivados , Animais , Anti-Helmínticos/administração & dosagem , Indústria de Laticínios/legislação & jurisprudência , Indústria de Laticínios/tendências , União Europeia , Feminino , Cabras , Ivermectina/administração & dosagem
3.
J Chem Ecol ; 46(1): 99-113, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31845136

RESUMO

Goat herding is an important tool in the ecologically sound management of Mediterranean shrublands and woodlands, although effective levels of woody biomass removal by the goats is neither guaranteed nor easy to predict. Preliminary observations indicated that one reason for this may be poor understanding of plant-herbivore interactions that operate intraspecifically at the local spatial scale. We asked, whether goats show intraspecific preferences among neighboring plants when foraging a small local population of Pistacia lentiscus, a dominant tall shrub. First, we characterized and quantified the profile of stored and emitted volatile organic compounds (VOCs) and the PEG-binding capacity of tannins (a proxy for protein binding capacity) in the foliage of P. lentiscus shrubs, sampled within an area of 0.9 ha. We then tested goat preference between pairs of these shrubs that differed in chemical composition. Almost all sampled P. lentiscus shrubs were allocated to one of two distinct VOC chemotypes: one dominated by germacrene D and limonene (designated chemotype L) and the other by germacrene D and α-pinene (chemotype P). In contrast, continuous moderate variability was found in the binding capacity of tannins in the foliage. Goats showed preference for shrubs of chemotype L over those of chemotype P, and their preference was negatively correlated with the binding capacity of tannins. Possible influences of VOCs on goat preference that may explain the observed patterns are discussed in the light of possible context-dependent interpretation of plant VOC signals by large mammalian herbivores.


Assuntos
Herbivoria/efeitos dos fármacos , Pistacia/química , Taninos/farmacologia , Compostos Orgânicos Voláteis/farmacologia , Animais , Cromatografia Gasosa-Espectrometria de Massas , Cabras/fisiologia , Modelos Lineares , Pistacia/metabolismo , Folhas de Planta/química , Folhas de Planta/metabolismo , Análise de Componente Principal , Taninos/análise , Taninos/química , Compostos Orgânicos Voláteis/análise , Compostos Orgânicos Voláteis/química
4.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117392, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31330421

RESUMO

Curcumin the extract obtained from the dried rhizome of turmeric, Curcuma longa is a hydrophobic phenol that delivers numerous pharmacological actions like anti-inflammatory, anti-microbial and anti-oxidant, anti-psoriasis, antidiabetic, anticancer. But curcumin has low bioavailability issues that accompany low aqueous solubility, further, when administered orally, >90% of the drug degrades rapidly in the alkaline medium. Administering the drug topically can bypass the problem as well as first-pass metabolism and therefore delivering the drug at the targeted site of action. Encapsulating curcumin in nanostructured lipid nanocarriers (NLC) is an excellent novel strategy. Further, these NLC provides both the controlled release and helps in the enhanced permeation of the drug through the skin's physiological barrier, stratum corneum. For the NLC characterization, a reliable method must be developed that can accurately and precisely determine the drug content in the formulation and also for its in-vitro and ex-vivo characterization. This experiment describes the analytical validation parameters described as per International Conference of Harmonization guidelines to develop a method using the UV-Visible spectroscopy. The method was developed in two solvent systems i.e. methanol and 6.4 pH phosphate buffer with 1.5% polysorbate 80. Methanol solvent was used for the determination of curcumin in the NLC formulation via determining the encapsulation efficiency and 6.4 pH phosphate buffer with 1.5% polysorbate 80 solvent was used for in-vitro and ex-vivo characterization of the developed NLC formulation (cream and gel). These methods were validated in response to linearity, the limit of detection, the limit of quantification, precision, accuracy, repeatability, and specificity.


Assuntos
Curcumina/química , Portadores de Fármacos/química , Lipídeos/química , Nanoestruturas/química , Espectrofotometria Ultravioleta/métodos , Administração Tópica , Animais , Curcumina/administração & dosagem , Curcumina/farmacocinética , Portadores de Fármacos/administração & dosagem , Cabras , Limite de Detecção , Modelos Lineares , Reprodutibilidade dos Testes , Pele/química , Pele/metabolismo
5.
Food Chem ; 302: 125350, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31415999

RESUMO

The aim of the study was to investigate the use of serine protease from Yarrowia lipolytica yeast for reduction of milk proteins allergenicity. Whey protein concentrate (WPC-80), αs-casein and their hydrolysates were analyzed for the capacity to bind IgE and IgG antibodies present in sera from patients with cow milk protein allergy using a competitive ELISA. The hydrolysis of αs-casein and whey protein concentrate contributed to a significant reduction of their immunoreactive epitopes. In case of IgE antibodies, the lowest binding capacity was detected in the 24 h hydrolysates of both proteins in which the inhibition of the reaction was ≤20 and ≤68% for αs-casein and whey protein concentrate respectively. One hour hydrolysis of WPC-80 reduced the protein antigenicity, while the longer time (5 h) might lead to the exposure of new IgE - reactive epitopes.


Assuntos
Hipersensibilidade a Leite/imunologia , Proteínas do Leite/imunologia , Hidrolisados de Proteína/imunologia , Serina Proteases/metabolismo , Alérgenos/imunologia , Alérgenos/metabolismo , Animais , Caseínas/imunologia , Caseínas/metabolismo , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Epitopos , Feminino , Cabras/imunologia , Humanos , Imunoglobulina E/imunologia , Imunoglobulina E/metabolismo , Imunoglobulina G/imunologia , Imunoglobulina G/metabolismo , Proteínas do Leite/metabolismo , Hidrolisados de Proteína/metabolismo , Proteínas do Soro do Leite/imunologia , Proteínas do Soro do Leite/metabolismo , Yarrowia/enzimologia
6.
J Sci Food Agric ; 100(1): 325-334, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31584699

RESUMO

BACKGROUND: Meat fraud and adulteration incidents occur frequently in almost all regions of the globe, especially with the increase in the world's population. To ensure the authenticity of meat products, we developed a 10-plex xMAP assay to simultaneously detect ten animal materials: bovine, caprine, poultry, swine, donkey, deer, horse, dog, fox and mink. RESULTS: This method was investigated by analyzing DNA extracts from raw muscle, muscle mixtures, meat products and animal feeds. Our results indicated that the species of interest can be identified, differentiated and detected down to 1 g kg-1 in binary mixtures or 0.01-0.001 ng of genomic DNA from specific species. Testing of 125 commercial samples showed a 97.4% coincidence rate with the method used in routine testing in our lab. CONCLUSION: These results indicated that the method established in this study could detect ten animal materials simultaneously within 3 h, which provides a new, useful tool for animal ingredient analysis in meat products and animal feeds. © 2019 Society of Chemical Industry.


Assuntos
DNA Mitocondrial/genética , Contaminação de Alimentos/análise , Produtos da Carne/análise , Técnicas de Amplificação de Ácido Nucleico/métodos , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Ração Animal/análise , Animais , Bovinos , Cervos , Cães , Raposas , Cabras , Cavalos , Vison , Técnicas de Amplificação de Ácido Nucleico/instrumentação , Aves Domésticas , Suínos
7.
J Surg Res ; 245: 483-491, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31446190

RESUMO

BACKGROUND: This study investigated the efficacy of extracorporeal shock wave (ESW) application in stimulating dermal thickness, vascularity, and collagen synthesis of facial skin in a large animal model. MATERIALS AND METHODS: The facial skin of the maxillary and mandibular areas of goats (n = 6 per group) was treated with ESWs of different intensities (0.15 and 0.45 mJ/mm2; 1000 pulses). After 4 d, histology and immunohistochemistry were used to evaluate the following: dermal thickness, total number and abundance of microvessels, amount of type 1 collagen, and α-smooth muscle actin expression. RESULTS: Dermal thickness, number and abundance of microvessels, and collagen synthesis increased after ESW application at both intensities (each P < 0.05). When comparing ESW groups, the highest collagen abundance was seen after 0.15 mJ/mm2 (P = 0.034), whereas the highest number of microvessels was detected after treatment with 0.45 mJ/mm2 (P = 0.002). CONCLUSIONS: A single-session application of focused low-energy ESWs to facial skin can increase dermal thickness by stimulating collagen production and local microcirculation. These findings commend the technique for future investigation for pretreatment of local or microvascular skin flaps to enhance tissue healing.


Assuntos
Colágeno/metabolismo , Face/cirurgia , Ondas de Choque de Alta Energia/uso terapêutico , Neovascularização Fisiológica/efeitos da radiação , Pele/efeitos da radiação , Animais , Modelos Animais de Doenças , Cabras , Humanos , Masculino , Modelos Animais , Pele/irrigação sanguínea , Pele/metabolismo , Retalhos Cirúrgicos/irrigação sanguínea , Ferida Cirúrgica/radioterapia , Cicatrização/efeitos da radiação
8.
Food Chem ; 309: 125765, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31711812

RESUMO

Goat milk samples of three lactation stages (colostrum, mature and late milk) were collected from three farms and analyzed with an untargeted method based on UPLC-Q-Exactive Orbitrap Mass Spectrometry and multivariate statistics. A total of 14 lipid subclasses and 756 lipid molecules were identified in samples. Five lipid subclasses and 51 lipid molecules in milk were significantly different among different geographical origins. Two lipid subclasses and 26 lipid molecules were significantly different among different lactation stages. Combined with the partial least squares discriminant analysis results of lipid molecules with a VIP value (Variable Importance in the projection) higher than 1, totally 38 and 19 lipid molecules could be used as potential indicators to identify geographical origins and lactation stages, respectively. Based on six and five selected molecules, the correct rates of discrimination models for geographical origin and lactation stage respectively reached 100% and 96%.


Assuntos
/métodos , Lipídeos/análise , Leite/química , Animais , Cromatografia Líquida de Alta Pressão , Colostro/química , Análise Discriminante , Feminino , Cabras/metabolismo , Lactação , Análise dos Mínimos Quadrados , Espectrometria de Massas , Gravidez , Análise de Componente Principal
9.
Vet Parasitol ; 276: 108973, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31785456

RESUMO

The objective of this study was to measure the effect of the nutritional status of Creole goat kids on the host responses and the nematode population established after an experimental H. contortus infection. Fifty six kids were fed with 4 diets corresponding to 2 nutritional statuses: the low nutritional status (HAY, hay ad libitum and HB, Hay ad libitum + banana) and the high nutritional status (HS, hay ad libitum + soya meal and HSB, hay ad libitum + banana + soya meal). For each diet, 8 kids were experimentally infected with 10,000 H. contortus infective larvae (L3) and 6 kids were kept as non-infected controls. From the day of infection until 6 weeks post-infection, samples were collected to measure individual intake, total tract digestibility, parasitological and hematological parameters. The dry matter intake (DMI), the average daily gain (ADG), the crude protein (CP) and the digestible CP intake were higher in goats fed the HS and HSB diets, but no statistically significant interaction between the nutritional status and the infection was observed. The packed cell volume (PCV), the red blood cell counts (RBC) and the mean corpuscular volume (MCV) were higher with the HS and the HSB diets. In kids with the high nutritional status the nematode burden and pathophysiological impact of the infection were significantly lower but not the FEC. In conclusion, this reduced establishment rate was associated with an increased production of eggs by the female parasites and suggested a phenomenon of density-dependent prolificacy of H. contortus probably inherent to the fitness of the parasite population.


Assuntos
Doenças das Cabras/parasitologia , Hemoncose/veterinária , Haemonchus/crescimento & desenvolvimento , Estado Nutricional/fisiologia , Anemia/prevenção & controle , Anemia/veterinária , Ração Animal/análise , Ração Animal/normas , Animais , Dieta/veterinária , Digestão , Ingestão de Alimentos , Eosinófilos/citologia , Fezes/química , Fezes/parasitologia , Feminino , Doenças das Cabras/fisiopatologia , Doenças das Cabras/prevenção & controle , Cabras , Hemoncose/parasitologia , Hemoncose/fisiopatologia , Contagem de Leucócitos/veterinária , Masculino , Contagem de Ovos de Parasitas/veterinária , Pepsinogênio A/sangue
10.
Eur J Histochem ; 63(4)2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31833328

RESUMO

Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is activated by interleukin (IL)-6 and IL-10 that generate nearly opposing responses. The suppressor of cytokine signaling 3 (SOCS3) is the negative regulator of STAT3 and plays an important role in the negative regulation of the inflammatory process. Evidence has shown the importance of STAT3 and SOCS3 during implantation and normal pregnancy. However, little is known about the relationship of both factors under hyperglycemic condition. The aim of this study was to evaluate the placenta regions exhibiting immunopositivity for STAT3 and SOCS3 in hyperglycemic rats, as well as correlate these proteins with IL-10 and IL-6 levels. It was observed increased expression of STAT3 at the labyrinth (approximately 47% of increase compared to control) and junctional zone (approximately 32% of increase compared to control) from hyperglycemic placentas. Similar results were observed to SOCS3 (approximately 71% -labyrinth- and 53% -junctional zone- of increase compared to control). The levels of IL-10 were augmented at hyperglycemic placentas (approximately 1.5 fold of increase) and they were positively correlated with the increase of STAT3 at the labyrinth and SOCS at junctional zone. Therefore, under hyperglycemic conditions, the relation between STAT3 and SOCS3 was changed, leading to unbalance of the cytokine profile.


Assuntos
Hiperglicemia/metabolismo , Placenta/metabolismo , Fator de Transcrição STAT3/metabolismo , Proteína 3 Supressora da Sinalização de Citocinas/metabolismo , Animais , Anticorpos/imunologia , Feminino , Cabras , Hiperglicemia/patologia , Imuno-Histoquímica , Interleucina-10/metabolismo , Interleucina-6/metabolismo , Placenta/patologia , Gravidez , Coelhos , Ratos Wistar , Fator de Transcrição STAT3/imunologia , Proteína 3 Supressora da Sinalização de Citocinas/imunologia
11.
Eur J Histochem ; 63(4)2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31833329

RESUMO

In fluorescence microscopy, light radiation can be used to bleach fluorescent molecules in formalin-fixed and paraffin-embedded (FFPE) samples, in order to increase the ratio between signal of interest and background autofluorescence. We tested if the same principle can be exploited in bright field microscopy to bleach pigmented melanoma FFPE sections together with cell morphology maintenance. After dewaxing and rehydration, serial FFPE sections of a feline diffuse iris melanoma, a canine dermal melanoma, a gray horse dermal melanoma and a swine cutaneous melanoma were irradiated with visible light for 1, 2, 3, 4 and 5 days, prior to Hematoxylin and Eosin staining. Complete bleaching was obtained after 1-day treatment in feline and swine melanomas, while 2 and 3 days were required in canine and equine neoplasms, respectively. In all treated samples, cell morphology was maintained. Photo-induced bleaching combined with immunohistochemistry was tested after a 3-day photo-treatment using five different markers. According to the literature, in all samples neoplastic cells stained positive for vimentin, S100 and PNL2, while negative for FVIII and pancytokeratin. In conclusion, visible light can be effectively exploited to bleach pigmented melanoma FFPE sections prior to perform routine histochemical and immunohistochemical stains.


Assuntos
Melaninas/efeitos da radiação , Melanoma/patologia , Fotodegradação , Animais , Anticorpos Monoclonais Murinos/imunologia , Gatos , Cães , Formaldeído/química , Cabras , Cavalos , Imuno-Histoquímica , Luz , Melanoma/veterinária , Camundongos , Inclusão em Parafina , Projetos Piloto , Coelhos , Proteínas S100/imunologia , Suínos , Temperatura Ambiente , Vimentina/imunologia
12.
Int. microbiol ; 22(4): 429-435, dic. 2019. graf, tab
Artigo em Inglês | IBECS | ID: ibc-185061

RESUMO

Studies of the digestive microbiota of ruminant animals most often focus on the bacterial diversity in the rumen or the feces of the animals, but little is known about the diversity and functions of their distal intestine. Here, the bacterial microbiota of the distal intestinal tract of two goats and two camels was investigated by metagenomics techniques. The bacterial taxonomic diversity and carbohydrate-active enzyme profile were estimated for samples taken from the small intestine, the large intestine, and the rectum of each animal. The bacterial diversity and abundance in the small intestine were lower than in the rectal and large intestinal samples. Analysis of the carbohydrate-active enzyme profiles at each site revealed a comparatively low abundance of enzymes targeting xylan and cellulose in all animals examined, similar to what has been reported earlier for sheep and therefore suggesting that plant cell wall digestion probably takes place elsewhere, such as in the rumen


No disponible


Assuntos
Animais , Camelus , Metagenômica/métodos , Microbioma Gastrointestinal , Ativação Enzimática/genética , Cabras , Trato Gastrointestinal/microbiologia , Intestino Delgado/microbiologia , Intestino Grosso/microbiologia , Estômago de Ruminante/enzimologia , Estômago de Ruminante/microbiologia , Ruminantes/microbiologia
13.
Emerg Microbes Infect ; 8(1): 1679-1687, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31749409

RESUMO

Adenoviruses (AdVs) are major contributors to clinical illnesses. Novel human and animal AdVs continue to be identified and characterized. Comparative analyses using bioinformatic methods and Omics-based technologies allow insights into how these human pathogens have emerged and their potential for host cross-species transmission. Systematic review of literature published across ProQuest, Pubmed, and Web of Science databases for evidence of adenoviral zoonotic potential identified 589 citations. After removing duplicates, 327 citations were screened for relevance; of which, 74 articles received full-text reviews. Among these, 24 were included here, of which 16 demonstrated evidence of zoonotic transmission of AdVs. These documented instances of AdV crossing host species barriers between humans and non-human primate, bat, feline, swine, canine, ovine, and caprine. Eight studies sought to but did not find evidence of zoonosis. The findings demonstrate substantial evidence suggesting AdVs have previously and will continue crossing host species barriers. These have human health consequences both in terms of novel pathogen emergence and epidemic outbreaks, and of appropriate and safe use of non-human adenoviruses for therapeutics. As routine human clinical diagnostics may miss a novel cross-species adenovirus infection in humans, next generation sequencing or panspecies molecular diagnostics may be necessary to detect such incursions.


Assuntos
Infecções por Adenoviridae/transmissão , Infecções por Adenoviridae/veterinária , Adenoviridae/fisiologia , Zoonoses/transmissão , Adenoviridae/genética , Infecções por Adenoviridae/virologia , Animais , Gatos , Cães , Cabras , Especificidade de Hospedeiro , Humanos , Filogenia , Ovinos , Suínos , Zoonoses/virologia
14.
Parasit Vectors ; 12(1): 520, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31690347

RESUMO

BACKGROUND: Cysticercosis caused by the metacestode larval stage of Taenia hydatigena is a disease of veterinary and economic importance. A considerable level of genetic variation among isolates of different intermediate hosts and locations has been documented. Generally, data on the genetic population structure of T. hydatigena is scanty and lacking in Nigeria. Meanwhile, similar findings in other cestodes like Echinococcus spp. have been found to be of epidemiological importance. Our aim, therefore, was to characterize and compare the genetic diversity of T. hydatigena population in Nigeria based on three mitochondrial DNA markers as well as to assess the phylogenetic relationship with populations from other geographical regions. METHODS: In the present study, we described the genetic variation and diversity of T. hydatigena isolates from Nigerian sheep and goats using three full-length mitochondrial genes: the cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunit 1 (nad1), and NADH dehydrogenase subunit 5 (nad5). RESULTS: The median-joining network of concatenated cox1-nad1-nad5 sequences indicated that T. hydatigena metacestodes of sheep origin were genetically distinct from those obtained in goats and this was supported by high FST values of nad1, cox1, and concatenated cox1-nad1-nad5 sequences. Genetic variation was also found to be higher in isolates from goats than from sheep. CONCLUSIONS: To the best of our knowledge, the present study described the genetic variation of T. hydatigena population for the first time in Nigeria using full-length mitochondrial genes and suggests the existence of host-specific variants. The population indices of the different DNA markers suggest that analysis of long mitochondrial DNA fragments may provide more information on the molecular ecology of T. hydatigena. We recommend that future studies employ long mitochondrial DNA sequence in order to provide reliable data that would explain the extent of genetic variation in different hosts/locations and the biological and epidemiological significance.


Assuntos
Genes Mitocondriais , Doenças das Cabras/parasitologia , Doenças dos Ovinos/parasitologia , Taenia/genética , Teníase/veterinária , Animais , Complexo IV da Cadeia de Transporte de Elétrons/genética , Marcadores Genéticos , Variação Genética , Cabras , NADH Desidrogenase/genética , Nigéria , Filogenia , Ovinos , Taenia/classificação , Taenia/isolamento & purificação , Teníase/parasitologia
15.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 474-478, 2019 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-31713374

RESUMO

OBJECTIVE: To investigate the prevalence and molecular features of Cryptosporidium in sheep and goats from Anhui Province and neighboring provinces. METHODS: A total 832 and 781 fresh fecal samples were collected from seven large-scale sheep farms and ten large-scale goat farms in Anhui Province and neighboring provinces of Henan, Jiangsu and Shandong. The prevalence and species of Cryptosporidium were investigated in the fecal samples from the sheep and goats in the study areas using nested PCR assay based on the Cryptosporidium-specific SSU rDNA gene, and the subgenotypes of C. parvum and C. ubiquitum were characterized by amplification and sequencing of the 60 kDa glycoprotein (gp60) gene. RESULTS: The overall prevalence of Cryptosporidium was 5.8% (48/832) in sheep and 8.7% (68/781) in goats in Anhui Province and neighboring provinces, respectively. The SSU rDNA gene-based PCR assay identified C. xiaoi and C. ubiquitum in sheep and C. parvum in goats, and subtyping revealed that all C. ubiquitum subgenotypes belonged to XIIa subtype 2 and C. parvum subgenotypes belonged to IIdA19G1. CONCLUSIONS: The identification of zoonotic C. ubiquitum XIIa subtype 2 and C. parvum subtype IIdA19G1 suggests that sheep and goats may serve as a potential source for human Cryptosporidium infections.


Assuntos
Criptosporidiose , Cryptosporidium , Doenças das Cabras , Doenças dos Ovinos , Animais , Criptosporidiose/epidemiologia , Criptosporidiose/parasitologia , Cryptosporidium/classificação , Cryptosporidium/genética , Fezes/parasitologia , Genótipo , Doenças das Cabras/epidemiologia , Doenças das Cabras/parasitologia , Cabras , Humanos , Reação em Cadeia da Polimerase , Prevalência , RNA Ribossômico 18S/genética , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/parasitologia
16.
BMC Surg ; 19(1): 147, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31640661

RESUMO

BACKGROUND: We aimed to determine the optimal bipolar electrocoagulation power for laparoscopic surgery and to investigate which method, bipolar electrocoagulation, advanced bipolar, or ultrasonic technique was more reliable. METHODS: Goat mesenteric vessels (210 in vivo samples) with diameters of 3.03-5.44 mm were selected. Bipolar electrocoagulation with 80 W, 75 W, 70 W, 65 W, 60 W, 55 W, and 50 W, and advanced bipolar and ultrasonic techniques were performed on mesenteric vessels. The thermal damage width, hemostatic effect, and burst pressure of these tissues were recorded. SPSS version 13.0 was used for all data analysis. RESULTS: The results showed that 60 W was the optimal for bipolar electrocoagulation based on the thermal damage width, hemostatic effect, and burst pressure. In contrast, the thermal damage width of advanced bipolar and ultrasonic techniques was smaller than that of bipolar electrocoagulation, and advanced bipolartechnique had the highest successful rate for hemostasis and highest burst pressure. CONCLUSIONS: Bipolar electrocoagulation was optimally performed with 60 W of power. Compared with ultrasonic and bipolar electrocoagulation techniques, advanced bipolar use was more reliable for mesenteric vessels in laparoscopic surgery; however, bipolar electrocoagulation with optimal power can be used for its simplicity of operation and low cost.


Assuntos
Eletrocoagulação/métodos , Hemostasia Cirúrgica/métodos , Laparoscopia/métodos , Animais , Cabras , Hemostasia , Humanos
17.
AAPS PharmSciTech ; 20(8): 325, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31659563

RESUMO

Localized intra-pocket, retentive, biodegradable, prolonged release thiolated membrane can provide an improved therapeutic efficacy of doxycycline at the site of action with evading off target side effects. To this end, thiolated chitosan-hyaluronic acid composite polymeric complex next-generation of the periodontal membrane was manufactured by solvent casting method. FTIR spectroscopic analysis displayed successful immobilization of thiol groups on the manufactured thiolated periodontal membrane. Moreover, XRD, DSC, AFM and TGA of the membrane confirmed the compatibility of ingredients and modifications in surface chemistry. The thiolated periodontal film was also investigated in terms of thickness, weight uniformity, water-uptake capacity, drug content, pH, entrapment efficiency, lysozymal degradation and release patterns. Also, mucoadhesion profile was explored on gingival mucosa. The immobilized thiol groups on thiolated chitosan and thiolated hyaluronate were found to be 168 ± 11 µM/g (mean ± SD, n = 3) and 189 ± 8 µM/g (mean ± SD, n = 3) respectively. Swelling capacity of the thiolated periodontal membrane was significantly ∼2-fold higher (p < 0.05) as compared to unmodified membrane. The obtained thiolated membrane depicted 3 -old higher mucoadhesive features as compared to the un-modified membrane. In vitro release kinetics indicated approximately more than 80% prolonged release within 7 days. Mechanical strength of the Thiolated bandage was also significantly ∼2-fold higher (p < 0.05) as compared to unmodified membrane. Ex-vivo retention study revealed enhanced retention of thiolated membrane as compared to unmodified membrane. In-vitro antimicrobial studies demonstrated that thiolated membrane could efficiently kill Porphyromonas gingivalis cells as compared to the native membrane. Moreover, ex-vivo biodegradation results indicated that 90% of the thiolated membrane was biodegradable in 28 days. Based on these findings, thiolated next-generation of the periodontal membrane seems to be promising for periodontitis therapy.


Assuntos
Antibacterianos/administração & dosagem , Doxiciclina/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Bolsa Periodontal/tratamento farmacológico , Compostos de Sulfidrila/administração & dosagem , Adulto , Animais , Antibacterianos/metabolismo , Doxiciclina/química , Doxiciclina/metabolismo , Composição de Medicamentos , Avaliação Pré-Clínica de Medicamentos/métodos , Cabras , Humanos , Bolsa Periodontal/metabolismo , Compostos de Sulfidrila/química , Compostos de Sulfidrila/metabolismo , Adulto Jovem
18.
Ann Parasitol ; 65(3): 245-250, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31599540

RESUMO

The aim of the study was to determine the prevalence and intensity of parasite infection of the digestive tract in sheep and goats from the West Pomerania region following anti-parasite treatment. Feces were freely collected from sheep and goats kept on organic farms and subjected to analysis by the Willis-Schlaf and McMaster's flotation methods. The mean extensity of infection by gastrointestinal parasites in both sheep and goats was found to be 100%. Both sheep and goats displayed the presence of gastrointestinal nematodes, Eimeria protozoa and Moniezia spp. tapeworms. The intensity of infection of sheep and goats by these parasites was arranged as follows: gastrointestinal nematodes > Eimeria protozoa > Moniezia tapeworms. Our findings confirm that a problem exists regarding the occurrence of parasites of the digestive tract among sheep and goats kept under organic conditions. Therefore, correct implementation of prophylactic programmes should be emphasised to ensure the control or elimination of parasites in animals, particularly those kept on pasture.


Assuntos
Gastroenteropatias , Doenças das Cabras , Parasitos , Doenças dos Ovinos , Animais , Fezes/parasitologia , Gastroenteropatias/epidemiologia , Gastroenteropatias/parasitologia , Gastroenteropatias/veterinária , Cabras , Agricultura Orgânica/estatística & dados numéricos , Contagem de Ovos de Parasitas , Polônia , Prevalência , Ovinos
19.
DNA Cell Biol ; 38(11): 1188-1196, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31603699

RESUMO

The mammary gland is an important organ for lactation in dairy goats. Mammary gland development and lactation functions are primarily regulated by natural hormones and certain crucial regulatory factors. Nedd4 family-interacting protein 1 (Ndfip1) can specifically bind to neural precursor cell-expressed, developmentally downregulated protein 4 (Nedd4) family members to participate in ubiquitination, which in turn regulates a range of biological processes in the body. However, the effects of Ndfip1 expression regulation at the post-transcriptional level on the development of mammary gland cells have not been previously reported. To study the regulation of Ndfip1 at post-transcriptional level, the overexpression and interference vectors of Ndfip1 were constructed, and co-transfected into the primary mammary gland epithelial cells cultured in vitro with miR-143 mimics and inhibitor. Dual luciferase reporter gene system, real-time quantitative polymerase chain reaction, western blotting, cholecystokinin octapeptide assays, and flow cytometry were used to identify their regulation and function. As a result, Ndfip1 was targeted and regulated by miR-143, which influences the development of mammary gland epithelial cells in dairy goats cultured in vitro. This study will lay an experimental foundation for further understanding the functions of Ndfip1 and miR-143.


Assuntos
Apoptose/genética , Células Epiteliais/fisiologia , Cabras , Lactação/genética , Glândulas Mamárias Animais/fisiologia , Proteínas de Membrana/genética , MicroRNAs/fisiologia , Animais , Células Cultivadas , Indústria de Laticínios , Células Epiteliais/metabolismo , Feminino , Regulação da Expressão Gênica , Cabras/genética , Cabras/metabolismo , Glândulas Mamárias Animais/citologia , Glândulas Mamárias Animais/metabolismo
20.
Yi Chuan ; 41(10): 928-938, 2019 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-31624055

RESUMO

The goat genome is the research basis for the protection and utilization of goat resources, which is important for breeding and improving goat breeds. At present, with the continuous improvement of goat reference genome, various important research progress in goat origin, evolution and adaptability has been achieved. In this review, we summarize the research progress in the goat genome in detail, encompassing goat genome structure, genome map (genetic, physical and comparative maps), goat high throughput sequencing and SNP chip development. We aim to provide a theoretical foundation for the development of goat genome selection.


Assuntos
Mapeamento Cromossômico , Genoma , Cabras/genética , Animais , Cruzamento
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