Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 38.244
Filtrar
1.
Nat Commun ; 15(1): 5450, 2024 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-38982039

RESUMO

Cultured meat is emerging as a new type of food that can provide animal protein in a sustainable way. Many previous studies employed various types of scaffolds to develop cultured meat with similar properties to slaughtered meat. However, important properties such as flavor were not discussed, even though they determine the quality of food. Flavor characteristics vary dramatically depending on the amount and types of amino acids and sugars that produce volatile compounds through the Maillard reaction upon cooking. In this study, a flavor-switchable scaffold is developed to release meaty flavor compounds only upon cooking temperature mimicking the Maillard reaction of slaughtered meat. By introducing a switchable flavor compound (SFC) into a gelatin-based hydrogel, we fabricate a functional scaffold that can enhance the aromatic properties of cultured meat. The temperature-responsive SFC stably remains in the scaffold during the cell culture period and can be released at the cooking temperature. Surprisingly, cultured meat fabricated with this flavor-switchable scaffold exhibits a flavor pattern similar to that of beef. This research suggests a strategy to develop cultured meat with enhanced sensorial characteristics by developing a functional scaffold which can mimic the natural cooking flavors of conventional meat.


Assuntos
Culinária , Aromatizantes , Reação de Maillard , Carne , Animais , Carne/análise , Aromatizantes/química , Paladar , Bovinos , Hidrogéis/química , Humanos , Alicerces Teciduais/química , Temperatura , Gelatina/química , Carne in vitro
2.
Anim Sci J ; 95(1): e13977, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38982658

RESUMO

Hard meat has low market value; hence, we used bittern as a novel meat tenderizer for bovine M. semitendinosus, one of a hard muscle. We investigated the effects of beef immersion in bittern, a basic solution primarily comprising MgCl2, on textural properties and water-holding capacity. Muscle samples from M. semitendinosus of Holstein steers were immersed in seven different solutions (RO, NaCl, MgCl2, red wine, pH 3, bittern, and pH 8) and heated at 80°C for 5min. The pH of the beef and immersion solutions, water-holding capacity, and maximum load of the meat were measured. Although beef immersed in red wine (pH 3) had a lower pH and water-holding capacity, that immersed in bittern (pH 8.4) had a higher pH and higher water holding capacity. These results indicate that immersion in acidic red wine may harden beef and that immersion in basic bittern may be more effective in maintaining water-holding capacity and softening beef.


Assuntos
Manipulação de Alimentos , Carne Vermelha , Água , Vinho , Animais , Bovinos , Concentração de Íons de Hidrogênio , Vinho/análise , Manipulação de Alimentos/métodos , Carne Vermelha/análise , Imersão , Qualidade dos Alimentos , Músculo Esquelético , Fenômenos Químicos , Masculino , Soluções , Temperatura Alta , Carne/análise , Dureza
3.
BMC Microbiol ; 24(1): 248, 2024 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-38971718

RESUMO

BACKGROUND: The usage of fluoroquinolones in Norwegian livestock production is very low, including in broiler production. Historically, quinolone-resistant Escherichia coli (QREC) isolated from Norwegian production animals rarely occur. However, with the introduction of a selective screening method for QREC in the Norwegian monitoring programme for antimicrobial resistance in the veterinary sector in 2014; 89.5% of broiler caecal samples and 70.7% of broiler meat samples were positive. This triggered the concern if there could be possible links between broiler and human reservoirs of QREC. We are addressing this by characterizing genomes of QREC from humans (healthy carriers and patients) and broiler isolates (meat and caecum). RESULTS: The most frequent mechanism for quinolone resistance in both broiler and human E. coli isolates were mutations in the chromosomally located gyrA and parC genes, although plasmid mediated quinolone resistance (PMQR) was also identified. There was some relatedness of the isolates within human and broiler groups, but little between these two groups. Further, some overlap was seen for isolates with the same sequence type isolated from broiler and humans, but overall, the SNP distance was high. CONCLUSION: Based on data from this study, QREC from broiler makes a limited contribution to the incidence of QREC in humans in Norway.


Assuntos
Antibacterianos , Galinhas , Farmacorresistência Bacteriana , Infecções por Escherichia coli , Escherichia coli , Quinolonas , Animais , Galinhas/microbiologia , Escherichia coli/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Humanos , Noruega , Infecções por Escherichia coli/veterinária , Infecções por Escherichia coli/microbiologia , Farmacorresistência Bacteriana/genética , Quinolonas/farmacologia , Antibacterianos/farmacologia , Genômica , Plasmídeos/genética , Doenças das Aves Domésticas/microbiologia , Testes de Sensibilidade Microbiana , Genoma Bacteriano/genética , DNA Girase/genética , DNA Topoisomerase IV/genética , Carne/microbiologia , Mutação , Proteínas de Escherichia coli/genética , Ceco/microbiologia
4.
PeerJ ; 12: e17572, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38952978

RESUMO

The bioaccessibility of tannins as antioxidants in meat is essential to maximise their effectiveness in protecting the product. This property determines the amount of tannins available to interact with meat components, inhibiting lipid and protein oxidation and, consequently, prolonging shelf life and preserving the sensory quality of the product. The objective of this study was to evaluate the bioaccessibility of condensed tannins (CT) from Acacia mearnsii extract (AME) and their effect on the physico-chemical characteristics of fattened lamb meat. Thirty-six Dorset × Hampshire lambs (3 months old and 20.8 ± 3.3 kg live weight) were used. The lambs were distributed equally (n = 9) into four treatments: T1, T2, T3 and T4, which included a basal diet plus 0%, 0.25%, 0.5% and 0.75% of CT from AME, respectively. At the end of the fattening period, bioaccessibility was evaluated, the animals were slaughtered and a sample of the longissimus dorsi (LD) muscle was collected to assess colour, lipid oxidation, cooking weight loss and shear force on days 1, 4, 7 and 14 of shelf-life, in samples preserved at -20 °C. In addition, the long chain fatty acid profile was analysed. A completely randomised design was used, and the means were compared with Tukey's test (P < 0.05). The mean lightness (L*), yellowness (b*) and hue (H*) values were higher for T3 and T4. The addition of CT did not affect (P > 0.05) redness (a*), cooking weight loss (CWL) or shear force (SF). T4 decreased (P < 0.05) stearic acid and increased cis-9 trans-12 conjugated linoleic acid (CLA). Bioaccessibility was higher in the supplemented groups (T1 < T2, T3 and T4). In conclusion, supplementing CT from AME in the diet of lambs did not reduce lipid oxidation, but T3 or T4 improved some aspects of meat colour and CLA deposition.


Assuntos
Proantocianidinas , Animais , Ovinos , Proantocianidinas/farmacocinética , Antioxidantes/farmacocinética , Disponibilidade Biológica , Carne Vermelha/análise , Carne/análise , Culinária , Extratos Vegetais/química , Músculo Esquelético/metabolismo , Músculo Esquelético/química
5.
Arch Microbiol ; 206(7): 335, 2024 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-38953983

RESUMO

Salmonella is considered as one of the most common zoonotic /foodborne pathogens in the world. The application of bacteriophages as novel antibacterial agents in food substrates has become an emerging strategy. Bacteriophages have the potential to control Salmonella contamination.We have isolated and characterized a broad-spectrum Salmonella phage, SP154, which can lyse 9 serotypes, including S. Enteritidis, S. Typhimurium, S. Pullorum, S. Arizonae, S. Dublin, S. Cholerasuis, S. Chester, S. 1, 4, [5], 12: i: -, and S. Derby, accounting for 81.9% of 144 isolates. SP154 showed a short latent period (40 min) and a high burst size (with the first rapid burst size at 107 PFUs/cell and the second rapid burst size at approximately 40 PFUs/cell). Furthermore, SP154 activity has higher survival rates across various environmental conditions, including pH 4.0-12.0 and temperatures ranging from 4 to 50 °C for 60 min, making it suitable for diverse food processing and storage applications. Significant reductions in live Salmonella were observed in different foods matrices such as milk and chicken meat, with a decrease of up to 1.9 log10 CFU/mL in milk contamination and a 1 log10 CFU/mL reduction in chicken meat. Whole genome sequencing analysis revealed that SP154 belongs to the genus Ithacavirus, subfamily Humphriesvirinae, within the family Schitoviridae. Phylogenetic analysis based on the terminase large subunit supported this classification, although an alternate tree using the tail spike protein gene suggested affiliation with the genus Kuttervirus, underscoring the limitations of relying on a single gene for phylogenetic inference. Importantly, no virulence or antibiotic resistance genes were detected in SP154. Our research highlights the potential of using SP154 for biocontrol of Salmonella in the food industry.


Assuntos
Microbiologia de Alimentos , Genoma Viral , Fagos de Salmonella , Salmonella , Sequenciamento Completo do Genoma , Fagos de Salmonella/genética , Fagos de Salmonella/isolamento & purificação , Fagos de Salmonella/classificação , Fagos de Salmonella/fisiologia , Animais , Salmonella/virologia , Salmonella/genética , Salmonella/classificação , Salmonella/isolamento & purificação , Galinhas , Leite/microbiologia , Leite/virologia , Carne/microbiologia , Carne/virologia , Filogenia
6.
BMC Genomics ; 25(1): 654, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38956457

RESUMO

BACKGROUND: Carcass weight (HCW) and marbling (MARB) are critical for meat quality and market value in beef cattle. In composite breeds like Brangus, which meld the genetics of Angus and Brahman, SNP-based analyses have illuminated some genetic influences on these traits, but they fall short in fully capturing the nuanced effects of breed of origin alleles (BOA) on these traits. Focus on the impacts of BOA on phenotypic features within Brangus populations can result in a more profound understanding of the specific influences of Angus and Brahman genetics. Moreover, the consideration of BOA becomes particularly significant when evaluating dominance effects contributing to heterosis in crossbred populations. BOA provides a more comprehensive measure of heterosis due to its ability to differentiate the distinct genetic contributions originating from each parent breed. This detailed understanding of genetic effects is essential for making informed breeding decisions to optimize the benefits of heterosis in composite breeds like Brangus. OBJECTIVE: This study aims to identify quantitative trait loci (QTL) influencing HCW and MARB by utilizing SNP and BOA information, incorporating additive, dominance, and overdominance effects within a multi-generational Brangus commercial herd. METHODS: We analyzed phenotypic data from 1,066 genotyped Brangus steers. BOA inference was performed using LAMP-LD software using Angus and Brahman reference sets. SNP-based and BOA-based GWAS were then conducted considering additive, dominance, and overdominance models. RESULTS: The study identified numerous QTLs for HCW and MARB. A notable QTL for HCW was associated to the SGCB gene, pivotal for muscle growth, and was identified solely in the BOA GWAS. Several BOA GWAS QTLs exhibited a dominance effect underscoring their importance in estimating heterosis. CONCLUSIONS: Our findings demonstrate that SNP-based methods may not detect all genetic variation affecting economically important traits in composite breeds. BOA inclusion in genomic evaluations is crucial for identifying genetic regions contributing to trait variation and for understanding the dominance value underpinning heterosis. By considering BOA, we gain a deeper understanding of genetic interactions and heterosis, which is integral to advancing breeding programs. The incorporation of BOA is recommended for comprehensive genomic evaluations to optimize trait improvements in crossbred cattle populations.


Assuntos
Cruzamento , Estudo de Associação Genômica Ampla , Fenótipo , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas , Animais , Bovinos/genética , Genótipo , Vigor Híbrido , Carne , Alelos
7.
PLoS One ; 19(7): e0306648, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38968240

RESUMO

BACKGROUND: Children are recommended to consume animal source foods (ASF) as part of diversified diets. However, ASF consumption practice of infant and young children (IYC) is less studied and contributing factors are not exhaustively identified. Therefore, the purpose of this study is to assess consumption of ASF and identify associated factors among 6-23 months old IYC from selected rural districts in Ethiopia. METHODS: A cross-sectional study was conducted in a total of 606 IYC from selected rural districts of Oromia and Sidama regional states in Ethiopia. A two-stage sampling technique was used to select participants. Data were collected using interviewer-administered questionnaire. Frequencies, percentages and mean scores with standard deviations were generated to describe participants and report univariate outcomes. Consumption of ASF was assessed using a 24-hour dietary recall. Logistic regression analysis was applied to identify contributing factors contributed to IYC's ASF consumption practice. RESULTS: Dairy, eggs and meat were consumed by 41.2%, 16.4% and 2.3% of IYC, respectively. Household food security increased odds of dairy [AOR = 1.66 (95%CI: 1.16 2.38), P = 0.006], eggs [AOR = 2.15 (95%CI: 1.33, 3.49), P = 0.002] and meat [AOR = 5.08 (95%CI: 1.09, 23.71), P = 0.039] consumption. Cow [AOR = 1.86 (95%CI: 1.28, 2.70), P = 0.001], donkey [AOR = 1.83 (95%CI: 1.08, 3.11), P = 0.024] and chicken [AOR = 1.53 (95%CI: 1.05, 2.22), P = 0.027] ownership increased the odds of dairy consumption. Grades 5-8 [AOR = 1.74 (95%CI: 1.06, 2.86), P = 0.028] or ≥9 [AOR = 2.96 (95%CI: 1.62, 5.42), P <0.001] maternal educational achievements were also associated with better dairy consumption. Children from households that owned chicken [AOR = 3.20 (95%CI: 1.97, 5.19), P <0.001] or produce root crops [AOR = 1.67 (95%CI: 1.05, 2.66), P = 0.031] were with increased odds to consume eggs. CONCLUSIONS: Low proportion of children consumed ASF. Household food security, livestock ownership, household income, root crop production and maternal education contributed to ASF consumption. Nutrition sensitive agricultural extension activities aided by nutrition education should be considered and evaluated for their effect on IYC's ASF consumption practice.


Assuntos
Ovos , População Rural , Humanos , Etiópia , Lactente , Estudos Transversais , Masculino , Feminino , População Rural/estatística & dados numéricos , Animais , Carne , Laticínios , Dieta , Comportamento Alimentar , Inquéritos e Questionários
8.
Braz J Biol ; 84: e285337, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38985063

RESUMO

The article presents the results of scientific research on the production of lamb in the conditions of the Akmola region. The experiment was conducted on purebred Kazakh short-tailed rough-haired and cross-bred sheep obtained from industrial crossing of Kazakh short-tailed rough-haired queens with sheep- producers of the hampshire breed. Further, the article presents the results of feeding and fattening, as well as the morphological composition of the carcass of sheep of the Kazakh coarse-haired sheep breed of different ages. The object of the study for feeding and fattening were three groups of experimental sheep of the above-mentioned breed, where compound feed was included in the diet of the I experimental group in the form of top dressing, in the II experimental group, grain waste was also used for top dressing, and the control group was kept in the feed without top dressing. Based on the study of the feeding of experimental sheep, it was found that the absolute increase during the feeding period in experimental sheep who received fertilization in the form of compound feed is higher than in their peers, respectively, by 0.2 and 1.49 kg. In terms of morphological and varietal composition, in particular, the number of cuts of grade 1, there was also an advantage of experimental sheep that received fertilization in the form of compound feed compared to their peers, respectively, by 2.3-8.3%. In general, the results of a study on the growth, development and slaughter qualities of experimental sheep, i.e. purebred Kazakh short-tailed rough-haired and crossbred, obtained from crossing with sheep producers of the precocious breed "hampshire" showed that from the moment of birth to 2 and 4 months. For example, crossbred sheep were slightly superior to purebred peers in terms of the studied indicators. In particular, the results of feeding sheep of the Kazakh coarse-haired broad-tailed breed from 4 to 6 months, depending on the top dressing.


Assuntos
Ração Animal , Carne , Animais , Cazaquistão , Ovinos/crescimento & desenvolvimento , Ovinos/classificação , Carne/análise , Ração Animal/análise , Feminino , Masculino , Clima , Criação de Animais Domésticos
9.
Food Chem ; 455: 139958, 2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-38850992

RESUMO

The feasibility of Near Infrared Spectroscopy was assessed for aging traceability of steaks of Angus beef (Biceps femoris) individually vacuum-packaged, as well as for the prediction of the refrigeration storage time (0, 7, and 14 days). For this purpose, a total of 288 steaks homogeneously distributed among the sampling times were used. The model developed by Partial Least Squares-Discriminant Analysis offered high discrimination ability between aged beef vs. non-aged. The accuracy after external validation exceeded 90%. Regarding the predictive capacity of the storage time, it was greater on the set of aged samples, in which the accuracy achieved values higher than 96%, while the accuracy decreased to 75% for the non-aged samples. Results obtained support the ability of NIRS technology to be considered in any digital transformation strategy for traceability across the meat supply chain.


Assuntos
Espectroscopia de Luz Próxima ao Infravermelho , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Espectroscopia de Luz Próxima ao Infravermelho/instrumentação , Animais , Bovinos , Carne/análise , Armazenamento de Alimentos
10.
Sci Rep ; 14(1): 13033, 2024 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-38871837

RESUMO

Men tend to eat more meat than women, but it is not clear why. We tested three hypotheses in a cross-cultural design (20,802 individuals in 23 countries across four continents): that gender differences are (a) universal, (b) related to gender roles and thus weaker in countries with higher gender equality and human development, or (c) related to opportunities to express gender roles and thus stronger in countries with higher gender equality and human development. Across all countries, men tended to consume more meat than women. However, this difference increased significantly in countries with greater human development and gender equality. The paradoxical gender gap in meat consumption aligns with previous research that suggests greater differences in behavior across genders in contexts that are more developed and gender equal. We discuss implications for theories of culture and gender as well as practical implications for global meat reduction.


Assuntos
Carne , Feminino , Masculino , Humanos , Fatores Sexuais , Comparação Transcultural , Adulto , Comportamento Alimentar , Cultura , Papel de Gênero , Pessoa de Meia-Idade , Adulto Jovem
11.
Food Res Int ; 189: 114551, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38876590

RESUMO

During the cold chain storage process, changes in metabolites and microorganisms are highly likely to lead to changes in meat quality. To elucidate the changes in the composition of metabolites and microbiota during cold chain storage of mutton, this study utilized untargeted metabolome and 5R 16S rRNA sequencing analyses to investigate the changes in the longissimus dorsi under different cold chain temperatures (4 °C and -20 °C). With the extension of cold chain storage time, the meat color darkened and the content of C18:2n-6, C20:3n-6, and C23:0 were significantly increased in mutton. In this study, nine metabolites, including 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine, alanylphenylala-nine, indole-3-acrylic acid and the others, were significantly altered during cold chain storage. The abundance of the dominant microorganisms, including Brachymonas, Aeromonas, Corynebacterium and Steroidobacter, was significantly altered. Furthermore, a high correlation was observed between the different metabolites and microorganisms. These findings provide an in-depth understanding of the effects of different cold chain storage temperatures and times on the quality of mutton.


Assuntos
Temperatura Baixa , Armazenamento de Alimentos , Armazenamento de Alimentos/métodos , Animais , Carne/microbiologia , RNA Ribossômico 16S/genética , Bactérias/genética , Bactérias/classificação , Bactérias/metabolismo , Microbiologia de Alimentos , Microbiota , Metaboloma , Refrigeração
12.
Food Res Int ; 189: 114549, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38876607

RESUMO

In cultured meat (CM) production, Scaffolding plays an important role by aiding cell adhesion, growth, differentiation, and alignment. The existence of fibrous microstructure in connective and muscle tissues has attracted considerable interest in the realm of tissue engineering and triggered the interest of researchers to implement scaffolding techniques. A wide array of research efforts is ongoing in scaffolding technologies for achieving the real meat structure on the principality of biomedical research and to replace serum free CM production. Scaffolds made of animal-derived biomaterials are found efficient in replicating the extracellular matrix (ECM), thus focus should be paid to utilize animal byproducts for this purpose. Proper identification and utilization of plant-derived scaffolding biomaterial could be helpful to add diversified options in addition to animal derived sources and reduce in cost of CM production through scaffolds. Furthermore, techniques like electrospinning, modified electrospinning and 3D bioprinting should be focused on to create 3D porous scaffolds to mimic the ECM of the muscle tissue and form real meat-like structures. This review discusses recent advances in cutting edge scaffolding techniques and edible biomaterials related to structured CM production.


Assuntos
Matriz Extracelular , Engenharia Tecidual , Alicerces Teciduais , Alicerces Teciduais/química , Engenharia Tecidual/métodos , Animais , Matriz Extracelular/química , Carne , Materiais Biocompatíveis/química , Bioimpressão/métodos , Impressão Tridimensional , Carne in vitro
13.
Sci Rep ; 14(1): 13901, 2024 06 17.
Artigo em Inglês | MEDLINE | ID: mdl-38886454

RESUMO

Eucommia ulmoides is a traditional Chinese herbal medicine, with pharmacological effects such as lowering blood pressure and enhancing immune function. The effects of dietary Eucommia ulmoides polysaccharide (EUP) on immune function and meat quality were studied in Songliao Black Pigs. Blood lymphocyte counts and percentage, concentrations of serum total protein and of albumin increased, whereas those of urea nitrogen and triglyceride decreased. White blood cell and lymphocyte counts, and serum IgA, IgE, IgG2 a and IFN-γ increased. Average daily weight gain, slaughter weight, lean meat rate and cooked meat rate increased, whereas pH24, feed-weight ratio, fat rate, yellowness (b#) and centrifugal dehydration rate decreased. Transcriptome sequencing of longissimus dorsi muscle detected 32 differentially expressed genes (DEGs), of which 26 were up-regulated and 6 down-regulated. A total of 19 genes were differentially expressed in the four groups, 18 of which were up-regulated. The DEGs included ADAMTS4, PER1, STAC, SERPINE1, FASN, THRSP, SP7 and KRT80 and the protein interaction network showed 20 up-regulated nodes, three down-regulated nodes and 14 DEGs. GO functional annotation and enrichment analysis showed that 34 items were significantly enriched, including transferase activity, actin binding, acetyl coenzyme A, acyl coenzyme A metabolism, adipose tissue development and acyl glycerol homeostasis. KEGG pathway analysis showed that the AMPK and PPAR signaling pathways were enriched. Dietary Eucommia polysaccharide enhanced immune function in Songliao Black Pigs, improved growth and carcass performance, increased the expression of genes related to meat quality traits and improved meat quality.


Assuntos
Eucommiaceae , Polissacarídeos , Animais , Polissacarídeos/farmacologia , Eucommiaceae/química , Suínos , Ração Animal/análise , Carne/análise , Transcriptoma , Suplementos Nutricionais , Carne de Porco/análise , Perfilação da Expressão Gênica , Dieta/veterinária
14.
Physiol Rep ; 12(12): e16100, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38888088

RESUMO

This study investigated the effects of white meat, such as chicken, intake combined with resistance training on muscle mass and strength in the elderly women, and whether the underlying mechanism involves changes in the gut microbiota. Ninety-three volunteers (age 59-79 years) were randomly allocated to sedentary control with placebo (Sed + PL) or chicken meat (Sed + HP) and resistance training with placebo (RT + PL) or chicken meat (RT + HP). Resistance training sessions were performed 3 d/week for 12 weeks using leg extensions and curls. Boiled chicken meat (110 g, containing 22.5 g protein) was ingested 3 d/week for 12 weeks. Maximal muscle strength and whole-body lean mass increased significantly in the RT + PL group compared to the Sed + HP group, and the RT + HP group showed a significantly greater increase than the Sed + HP and RT + PL groups. Additionally, the gut microbiota composition did not change before or after the interventions in any of the four groups. Moreover, the individual comparison of gut bacteria using false discovery rate-based statistical analysis showed no alterations before or after the interventions in the four groups. Resistance training combined with chicken meat intake may effective have increased muscle mass and strength without drastically modifying the gut microbiota composition in elderly women.


Assuntos
Galinhas , Microbioma Gastrointestinal , Carne , Força Muscular , Músculo Esquelético , Treinamento Resistido , Humanos , Feminino , Microbioma Gastrointestinal/fisiologia , Treinamento Resistido/métodos , Idoso , Força Muscular/fisiologia , Pessoa de Meia-Idade , Animais , Músculo Esquelético/fisiologia
15.
Int J Mol Sci ; 25(11)2024 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-38891814

RESUMO

Copy number variation (CNV) serves as a significant source of genetic diversity in mammals and exerts substantial effects on various complex traits. Pingliang red cattle, an outstanding indigenous resource in China, possess remarkable breeding value attributed to their tender meat and superior marbling quality. However, the genetic mechanisms influencing carcass and meat quality traits in Pingliang red cattle are not well understood. We generated a comprehensive genome-wide CNV map for Pingliang red cattle using the GGP Bovine 100K SNP chip. A total of 755 copy number variable regions (CNVRs) spanning 81.03 Mb were identified, accounting for approximately 3.24% of the bovine autosomal genome. Among these, we discovered 270 potentially breed-specific CNVRs in Pingliang red cattle, including 143 gains, 73 losses, and 54 mixed events. Functional annotation analysis revealed significant associations between these specific CNVRs and important traits such as carcass and meat quality, reproduction, exterior traits, growth traits, and health traits. Additionally, our network and transcriptome analysis highlighted CACNA2D1, CYLD, UBXN2B, TG, NADK, and ITGA9 as promising candidate genes associated with carcass weight and intramuscular fat deposition. The current study presents a genome-wide CNV map in Pingliang red cattle, highlighting breed-specific CNVRs, and transcriptome findings provide valuable insights into the underlying genetic characteristics of Pingliang red cattle. These results offer potential avenues for enhancing meat quality through a targeted breeding program.


Assuntos
Variações do Número de Cópias de DNA , Estudo de Associação Genômica Ampla , Carne , Animais , Bovinos/genética , Variações do Número de Cópias de DNA/genética , Estudo de Associação Genômica Ampla/métodos , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas , Fenótipo , Cruzamento , Genoma , Qualidade dos Alimentos , Característica Quantitativa Herdável
16.
Int J Mol Sci ; 25(11)2024 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-38892012

RESUMO

A key element for the cost-effective development of cultured meat is a cell line culturable in serum-free conditions to reduce production costs. Heme supplementation in cultured meat mimics the original meat flavor and color. This study introduced a bacterial extract generated from Corynebacterium that was selected for high-heme expression by directed evolution. A normal porcine cell line, PK15, was used to apply the bacterial heme extract as a supplement. Consistent with prior research, we observed the cytotoxicity of PK15 to the heme extract at 10 mM or higher. However, after long-term exposure, PK15 adapted to tolerate up to 40 mM of heme. An RNA-seq analysis of these heme-adapted PK15 cells (PK15H) revealed a set of altered genes, mainly involved in cell proliferation, metabolism, and inflammation. We found that cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), lactoperoxidase (LPO), and glutathione peroxidase 5 (GPX5) were upregulated in the PK15H heme dose dependently. When we reduced serum serially from 2% to serum free, we derived the PK15H subpopulation that was transiently maintained with 5-10 mM heme extract. Altogether, our study reports a porcine cell culturable in high-heme media that can be maintained in serum-free conditions and proposes a marker gene that plays a critical role in this adaptation process.


Assuntos
Heme , Animais , Suínos , Heme/metabolismo , Linhagem Celular , Meios de Cultura Livres de Soro , Proliferação de Células/efeitos dos fármacos , Carne/análise , Citocromo P-450 CYP1A1/metabolismo , Citocromo P-450 CYP1A1/genética , Técnicas de Cultura de Células/métodos , Carne in vitro
17.
Int J Mol Sci ; 25(11)2024 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-38892214

RESUMO

Jeryak is the F1 generation of the cross between Gannan yak and Jersey cattle, which has the advantages of fast growth and high adaptability. The growth and development of skeletal muscle is closely linked to meat production and the quality of meat. However, the molecular regulatory mechanisms of muscle growth differences between Gannan yak and Jeryak analyzed from the perspective of chromatin opening have not been reported. In this study, ATAC-seq was used to analyze the difference of chromatin openness in longissimus muscle of Gannan yak and Jeryak. It was found that chromatin accessibility was more enriched in Jeryak compared to Gannan yak, especially in the range of the transcription start site (TSS) ± 2 kb. GO and KEGG enrichment analysis indicate that differential peak-associated genes are involved in the negative regulation of muscle adaptation and the Hippo signaling pathway. Integration analysis of ATAC-seq and RNA-seq revealed overlapping genes were significantly enriched during skeletal muscle cell differentiation and muscle organ morphogenesis. At the same time, we screened FOXO1, ZBED6, CRY2 and CFL2 for possible involvement in skeletal muscle development, constructed a genes and transcription factors network map, and found that some transcription factors (TFs), including YY1, KLF4, KLF5 and Bach1, were involved in skeletal muscle development. Overall, we have gained a comprehensive understanding of the key factors that impact skeletal muscle development in various breeds of cattle, providing new insights for future analysis of the molecular regulatory mechanisms involved in muscle growth and development.


Assuntos
Músculo Esquelético , RNA-Seq , Animais , Bovinos/genética , Músculo Esquelético/metabolismo , Músculo Esquelético/crescimento & desenvolvimento , Sequenciamento de Cromatina por Imunoprecipitação , Desenvolvimento Muscular/genética , Cromatina/genética , Cromatina/metabolismo , Carne/análise , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
18.
Food Chem ; 454: 139645, 2024 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-38833823

RESUMO

Herein, we investigated the potential of REIMS analysis for classifying muscle composition and meat sensory quality. The study utilized 116 samples from 29 crossbred Angus × Salers, across three muscle types. Prediction models were developed combining REIMS fingerprints and meat quality metrics. Varying efficacy was observed across REIMS discriminations - muscle type (71 %), marbling level (32 %), untrained consumer evaluated tenderness (36 %), flavor liking (99 %) and juiciness (99 %). Notably, REIMS demonstrated the ability to classify 116 beef across four Meat Standards Australia grades with an overall accuracy of 37 %. Specifically, "premium" beef could be differentiated from "unsatisfactory", "good everyday" and "better than everyday" grades with accuracies of 99 %, 84 %, and 62 %, respectively. Limited efficacy was observed however, in classifying trained panel evaluated sensory quality and fatty acid composition. Additionally, key predictive features were tentatively identified from the REIMS fingerprints primarily comprised of molecular ions present in lipids, phospholipids, and amino acids.


Assuntos
Paladar , Bovinos , Animais , Humanos , Espectrometria de Massas , Austrália , Ácidos Graxos/análise , Ácidos Graxos/química , Músculo Esquelético/química , Carne/análise , Aminoácidos/análise , Aminoácidos/química
19.
Ecol Food Nutr ; 63(4): 355-386, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38835162

RESUMO

Conformity to masculinity ideology predicts men's meat consumption and willingness to reduce their meat intake, but it is unknown which specific masculine norms account for these relationships. This study investigated which traditional and non-traditional masculine norms predict meat consumption, red and processed meat consumption, and willingness to reduce meat consumption in 557 Australian and English males. Men who support the use of physical violence and place high importance on sex ate more meat. Willingness to reduce was highest among men with gender egalitarian views. Targeting these specific masculine norms may be important for mitigating men's overconsumption of meat.


Assuntos
Masculinidade , Carne , Humanos , Masculino , Adulto , Adulto Jovem , Austrália , Pessoa de Meia-Idade , Dieta , Adolescente , Preferências Alimentares
20.
Food Res Int ; 190: 114566, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38945597

RESUMO

This study assessed water relaxometry of beef exposed to different ageing techniques by examining the inner and surface regions using time-domain nuclear magnetic resonance (TD-NMR) relaxometry. Beef strip loins were aged under vacuum (Wet), under vacuum using moisture absorbers (Abs), under vacuum using moisture absorbers and with mechanical tenderisation (AbsTend), or without any packaging (Dry). The ageing technique significantly influenced various meat parameters, including dehydration, total loss, and the moisture content of the meat surface. The transverse (T2) relaxation times provided a more sensitive indicator of the changes in meat water relaxometry than the longitudinal (T1) relaxation times. The Dry samples exhibited distinct differences in the T2 signals between the surface and inner regions of the meat. In particular, for the inner region, there were significant differences in signal areas between the Wet and Dry samples, and the Abs and AbsTend samples were positioned closely together between the Dry and Wet samples. The principal component analysis supported these findings: it indicated some differentiation among the ageing techniques in the score plot, but the differentiation was more pronounced when analysing the surface region. Additionally, there was a strong correlation between dehydration and the T2 values, leading to a clustering of the samples based on the ageing technique. The overlap between the Abs and AbsTend samples, situated between the Dry and Wet samples, suggests the potential of these treatments to produce meat with properties that are intermediate to Wet and Dry meat. Furthermore, tenderisation did not lead to greater dehydration.


Assuntos
Manipulação de Alimentos , Espectroscopia de Ressonância Magnética , Água , Água/química , Animais , Bovinos , Espectroscopia de Ressonância Magnética/métodos , Manipulação de Alimentos/métodos , Vácuo , Carne Vermelha/análise , Fatores de Tempo , Carne/análise , Análise de Componente Principal
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...