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2.
Sci Rep ; 14(1): 8023, 2024 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-38580805

RESUMO

Toxic metals are vital risk factors affecting serum ion balance; however, the effect of their co-exposure on serum ions and the underlying mechanism remain unclear. We assessed the correlations of single metal and mixed metals with serum ion levels, and the mediating effects of mineralocorticoids by investigating toxic metal concentrations in the blood, as well as the levels of representative mineralocorticoids, such as deoxycorticosterone (DOC), and serum ions in 471 participants from the Dongdagou-Xinglong cohort. In the single-exposure model, sodium and chloride levels were positively correlated with arsenic, selenium, cadmium, and lead levels and negatively correlated with zinc levels, whereas potassium and iron levels and the anion gap were positively correlated with zinc levels and negatively correlated with selenium, cadmium and lead levels (all P < 0.05). Similar results were obtained in the mixed exposure models considering all metals, and the major contributions of cadmium, lead, arsenic, and selenium were highlighted. Significant dose-response relationships were detected between levels of serum DOC and toxic metals and serum ions. Mediation analysis showed that serum DOC partially mediated the relationship of metals (especially mixed metals) with serum iron and anion gap by 8.3% and 8.6%, respectively. These findings suggest that single and mixed metal exposure interferes with the homeostasis of serum mineralocorticoids, which is also related to altered serum ion levels. Furthermore, serum DOC may remarkably affect toxic metal-related serum ion disturbances, providing clues for further study of health risks associated with these toxic metals.


Assuntos
Arsênio , Metais Pesados , Selênio , Humanos , Chumbo/toxicidade , Arsênio/toxicidade , Cádmio/toxicidade , Análise de Mediação , Mineralocorticoides , Intoxicação por Metais Pesados , Zinco , Ferro , Íons , China , Metais Pesados/toxicidade
3.
Environ Geochem Health ; 46(5): 165, 2024 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-38592368

RESUMO

Soil pollution around Pb-Zn smelters has attracted widespread attention around the world. In this study, we compiled a database of eight potentially toxic elements (PTEs) Pb, Zn, Cd, As, Cr, Ni, Cu, and Mn in the soil of Pb-Zn smelting areas by screening the published research papers from 2000 to 2023. The pollution assessment and risk screening of eight PTEs were carried out by geo-accumulation index (Igeo), potential ecological risk index (PERI) and health risk assessment model, and Monte Carlo simulation employed to further evaluate the probabilistic health risks. The results suggested that the mean values of the eight PTEs all exceeded the corresponding values in the upper crust, and more than 60% of the study sites had serious Pb and Cd pollution (Igeo > 4), with Brazil, Belgium, China, France and Slovenia having higher levels of pollution than other regions. Besides, PTEs in smelting area caused serious ecological risk (PERI = 10912.12), in which Cd was the main contributor to PREI (86.02%). The average hazard index (HI) of the eight PTEs for adults and children was 7.19 and 9.73, respectively, and the average value of total carcinogenic risk (TCR) was 4.20 × 10-3 and 8.05 × 10-4, respectively. Pb and As are the main contributors to non-carcinogenic risk, while Cu and As are the main contributors to carcinogenic risk. The probability of non-carcinogenic risk in adults and children was 84.05% and 97.57%, while carcinogenic risk was 92.56% and 79.73%, respectively. In summary, there are high ecological and health risks of PTEs in the soil of Pb-Zn smelting areas, and Pb, Cd, As and Cu are the key elements that cause contamination and risk, which need to be paid attention to and controlled. This study is expected to provide guidance for soil remediation in Pb-Zn smelting areas.


Assuntos
Cádmio , Chumbo , Adulto , Criança , Humanos , Chumbo/toxicidade , Carcinogênese , Carcinógenos , Poluição Ambiental , Probabilidade , Medição de Risco , Solo , Zinco
4.
Chemosphere ; 353: 141577, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38430937

RESUMO

Pollution in aquatic ecosystems has been increasing drastically worldwide changing their water quality. Therefore, species must be adapted to these new scenarios. In Aguascalientes City, four representative urban reservoirs contain lead in the water column and extremely high concentrations of sediments. Therefore, an analysis was conducted to evaluate the resilience of zooplankton species to lead exposure in each reservoir using dormant and organisms. Results demonstrated a decrease range from 57.5 to 22.5% in overall diapausing egg hatching rate, while survivorship rate also decreased from 98 to 54% when organisms were exposed to the water of the four reservoirs and increasing lead concentrations. When Pb exposure increased, results showed a global negative effect on both hatching rate (decreasing from 58 to 30% at 0.09 mg L-1) and survivorship levels (decreasing from 100% to 0.07% at 0.09 mg L-1). We provide Species Sensitivity Distribution for both water reservoir dilutions and lead concentration to analyze diapausing eggs hatching and survivorship of offspring in the presence of same polluted conditions or lead of the autochthonous species found in reservoirs. Furthermore, specific analysis with two populations of the cladoceran Moina macrocopa showed clear dissimilar hatching patterns that suggested a different adaptive mechanism. Niagara population shows a hatching rate of approximately 25% in the first two days of reservoir water exposure, while UAA population drastically increased hatching rate to 75% on exposure at day seven. We provide the first record of bioaccumulation in ephippia of M. macrocopa.


Assuntos
Cladóceros , Resiliência Psicológica , Rotíferos , Poluentes Químicos da Água , Animais , Chumbo/toxicidade , Ecossistema , México , Poluentes Químicos da Água/toxicidade , Eutrofização , Zooplâncton
5.
Sci Total Environ ; 923: 171497, 2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38453091

RESUMO

Lead (Pb) can disrupt plant gene expression, modify metabolite contents, and influence the growth of plants. Cuminum cyminum L. is highly adaptable to adversity, but molecular mechanism by which it responds to Pb stress is unknown. For this study, transcriptomic and metabolomic sequencing was performed on root tissues of C. cyminum under Pb stress. Our results showed that high Pb stress increased the activity of peroxidase (POD), the contents of malondialdehyde (MDA) and proline by 80.03 %, 174.46 % and 71.24 %, respectively. Meanwhile, Pb stress decreased the activities of superoxide dismutase (SOD) and catalase (CAT) as well as contents of soluble sugars and GSH, which thus affected the growth of C. cyminum. In addition, Pb stress influenced the accumulation and transport of Pb in C. cyminum. Metabolomic results showed that Pb stress affected eight metabolic pathways involving 108 differentially expressed metabolites, primarily amino acids, organic acids, and carbohydrates. The differentially expressed genes identified through transcriptome analysis were mainly involved the oxidation reductase activity, transmembrane transport, phytohormone signaling, and MAPK signaling pathway. The results of this study will help to understand the molecular mechanisms of C. cyminum response to Pb stress, and provide a basis for screening seeds with strong resistance to heavy metals.


Assuntos
Antioxidantes , Cuminum , Antioxidantes/metabolismo , Cuminum/química , Cuminum/metabolismo , Chumbo/toxicidade , Metabolômica , Perfilação da Expressão Gênica
6.
Environ Pollut ; 346: 123691, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38431245

RESUMO

The excessive accumulation of heavy metals, particularly lead (Pb) in agricultural soils, is a growing problem worldwide and needs urgent attention. This study aimed to prepare green silicon (Si) NPs using extract of Chenopodium quinoa leaves and evaluated their effects on Pb uptake and growth of maize (Zea mays L.). The results indicated that Pb exposure negatively affected the growth and chlorophyll contents of maize varieties, while SiNPs positively affected these attributes. Pb alone increased the electrolyte-leakage (EL), hydrogen-peroxide (H2O2) and selected antioxidant enzyme activities in leaves, whereas SiNPs decreased EL and H2O2 concentrations and further enhanced the enzyme activities as compared to their respective treatments without SiNPs. Pb-only treatments led to an increase in Pb concentrations and total Pb uptake in both shoots and roots. In contrast, SiNPs resulted in reduced Pb concentrations, with a concurrent decrease in total Pb uptake in shoots compared to the control treatment. The findings demonstrated that foliar application of SiNPs can mitigate the toxic effects of Pb in maize plants by triggering the antioxidant enzyme system and reducing the oxidative stress. Taken together, SiNPs have the potential to enhance maize production in Pb-contaminated soils. However, future research and application efforts should prioritize key aspects such as optimizing NPs synthesis, understanding positive mechanisms of green-synthesized NPs, and conducting multiple crop tests and real-world field trials.


Assuntos
Nanopartículas , Poluentes do Solo , Zea mays , Antioxidantes/farmacologia , Silício/farmacologia , Chumbo/toxicidade , Peróxido de Hidrogênio/farmacologia , Nanopartículas/toxicidade , Solo , Poluentes do Solo/análise
7.
Toxicology ; 503: 153771, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38452865

RESUMO

Lead (Pb2+) is a hazardous heavy metal that is pervasive in the human environment as a result of anthropogenic activity, and poses serious health risks, particularly in children. Due to its innumerable unique physical and chemical properties, it has various applications; therefore, it has become a common environmental pollutant. Lead may cause oxidative stress, and accumulating evidence indicates that oxidative stress influences the pathophysiology of lead poisoning, also called plumbism. The immune system is continually exposed to various environmental pathogens and xenobiotics, including heavy metals such as lead, and appears to be one of the most vulnerable targets. After being exposed to lead, cells are subjected to oxidative stress as a result of reactive oxygen species (ROS) production. When the generation and consumption of ROS are out of equilibrium, various cell structures, particularly phospholipids are disrupted leading to lipid peroxidation. Various inflammatory signalling pathways are activated as a consequence, along with reduced disease resistance, inflammation, autoimmunity, sensitization and disruption of the cell-mediated and humoral immune systems. Lead negatively affects the metabolism of cytokines, including the interleukins IL-2, IL-1b, IL-6, IL-4, IL-8, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN), as well as the expression and functioning of inflammatory enzymes such as cyclooxygenases. However, the cause of toxicity depends on the kind of lead, dosage, route of entry, exposure period, age, host and genetic predisposition.


Assuntos
Poluentes Ambientais , Intoxicação por Chumbo , Metais Pesados , Criança , Humanos , Citocinas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Chumbo/toxicidade , Metais Pesados/toxicidade , Poluentes Ambientais/toxicidade , Estresse Oxidativo
8.
Ecotoxicol Environ Saf ; 274: 116194, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38479312

RESUMO

Lead (Pb) is one of the most dreadful non-essential elements whose toxicity has been well reported worldwide due to its interference with the major plant functions and its overall yield. Bioremediation techniques comprising the application of beneficial microorganisms have gained attention in recent times owing to their ecofriendly nature. Addition of organic matter to soil has been reported to stimulate microbial activities. Compost application improves soil structure and binds toxic contaminants due to its larger surface area and presence of functional groups. Furthermore, it stimulates soil microbial activities by acting as C-source. So, in current study, we investigated the individual and synergistic potential of two lead (Pb)-tolerant Pseudomonas strains alongwith compost (1% w/w) in sustaining sunflower growth under Pb contaminated soil conditions. Lead chloride (PbCl2) salt was used for raising desired Pb concentration (500 mg kg-1). Results revealed that Pb stress drastically affected all the measured attributes of sunflower plant, however joint application of rhizobacteria and compost counteracted these adverse effects. Among them, co-application of str-1 and compost proved to be significantly better than str-2, as its inoculation significantly improved shoot and root lengths (64 and 76%), leaf area and leaves plant-1 (95 and 166%), 100-achene weight (200%), no. of flowers plant-1 (138%), chl 'a', 'b' and carotenoid (86, 159 and 33%) contents in sunflower as compared to control treatments. Furthermore, inoculation of Pseudomonas fluorescens along with compost increased the NPK in achene (139, 200 and 165%), flavonoid and phenolic contents (258 and 185%) along with transpiration and photosynthetic rates (54 and 72%) in leaves as compared to control treatment under Pb contamination. In addition, Pb entry to roots, shoots and achene were significantly suppressed under by 87, 90 and 91% respectively due to integrated application of compost and str-1 as evident by maximum Pb-immobilization efficiency (97%) obtained in this treatment. Similarly, bioconcentration factors for roots, shoots and achene were found to be 0.58, 0.18 and 0.0055 with associated translocation factor (0.30), which also revealed phytostabilization of Pb under combined application of PGPR and compost. Since, phytoremediation of heavy metals under current scenario of increasing global population is inevitable, results of the current study concluded that tolerant PGPR species along with organic amendments such as compost can inhibit Pb uptake by sunflower and confer Pb tolerance via improved nutrient uptake, physiology, antioxidative defense and gas exchange.


Assuntos
Compostagem , Helianthus , Poluentes do Solo , Antioxidantes/metabolismo , Helianthus/metabolismo , Pseudomonas/metabolismo , Chumbo/toxicidade , Chumbo/metabolismo , Biodegradação Ambiental , Raízes de Plantas/metabolismo , Solo/química , Nutrientes , Poluentes do Solo/análise
9.
J Wildl Dis ; 60(2): 285-297, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38345465

RESUMO

Lead (Pb) is a toxic nonessential metal, known mainly for causing poisoning of humans and wild birds. However, little is known about Pb exposure and its associated health effects in wild mammals. We conducted a global systematic literature review to identify peer-reviewed studies published on Pb exposure in wild mammalian species and the health effects they identified. In total, 183 studies, conducted in 35 countries and published over 62 yr (1961-2022), were included in the review. Only 6% (11/183) of the studies were conducted in developing countries. Although 153 mammalian species were studied, most studies focused on species that are easy to access (i.e., hunted species and small mammals that are easy to trap). Therefore, carnivores and scavengers were less frequently studied than herbivores and omnivores. Despite all studies reporting Pb concentrations, only 45 (25%) studies investigated health effects and, of these 45 studies, only 28 (62%) found any health effect in 57 species. All health effects were negative and ranged from subclinical effects to fatality. Methodologies of Pb sampling and quantification and reporting of results varied widely across the studies, making both Pb concentrations and health effects difficult to compare and evaluate. Thus, there is a need for more research on Pb exposure and its health effects on wild mammals, especially as carnivores and scavengers could be used as sentinels for ecosystem health.


Assuntos
Ecossistema , Chumbo , Humanos , Animais , Chumbo/toxicidade , Aves , Mamíferos , Animais Selvagens
10.
Sci Rep ; 14(1): 4372, 2024 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-38388664

RESUMO

Due to the characteristics of electrospun nanofibers (NFs), they are considered a suitable substrate for the adsorption and removal of heavy metals. Electrospun nanofibers are prepared based on optimized polycaprolactone (PCL, 12 wt%) and polyacrylic acid (PAA, 1 wt%) polymers loaded with graphene oxide nanoparticles (GO NPs, 1 wt%). The morphological, molecular interactions, crystallinity, thermal, hydrophobicity, and biocompatibility properties of NFs are characterized by spectroscopy (scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Thermogravimetric analysis), contact angle, and MTT tests. Finally, the adsorption efficacy of NFs to remove lead (Pb2+) from water and apple juice samples was determined using inductively coupled plasma optical emission spectroscopy (ICP-OES). The average diameter for PCL, PCL/PAA, and PCL/PAA/GO NFs was 137, 500, and 216 nm, respectively. Additionally, the contact angle for PCL, PCL/PAA, and PCL/PAA/GO NFs was obtained at 74.32º, 91.98º, and 94.59º, respectively. The cytotoxicity test has shown non-toxicity for fabricated NFs against the HUVEC endothelial cell line by more than 80% survival during 72 h. Under optimum conditions including pH (= 6), temperature (25 °C), Pb concentration (25 to 50 mg/L), and time (15 to 30 min), the adsorption efficiency was generally between 80 and 97%. The adsorption isotherm model of PCL/PAA/GO NFs in the adsorption of lead metal follows the Langmuir model, and the reaction kinetics follow the pseudo-second-order. PCL/PA/GO NFs have shown adsorption of over 80% in four consecutive cycles. The adsorption efficacy of NFs to remove Pb in apple juice has reached 76%. It is appropriate and useful to use these nanofibers as a high-efficiency adsorbent in water and food systems based on an analysis of their adsorption properties and how well they work.


Assuntos
Resinas Acrílicas , Água Potável , Grafite , Malus , Nanofibras , Poliésteres , Poluentes Químicos da Água , Água Potável/análise , Nanofibras/toxicidade , Nanofibras/química , Chumbo/toxicidade , Chumbo/análise , Adsorção , Poluentes Químicos da Água/análise , Cinética , Espectroscopia de Infravermelho com Transformada de Fourier
11.
Sci Total Environ ; 919: 170790, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38331279

RESUMO

The combined pollution of lead (Pb) and polystyrene microplastics (PS-MPs) is common in aquatic environments. However, the combined neurotoxicity of these two pollutants is still poorly understood. In this study, zebrafish (Danio rerio) larvae were used to assess the combined neurotoxicity and mechanism of Pb and PS-MPs at environmentally relevant concentrations. The results showed that Pb (10 µg/L) induced abnormal behavior including significantly reduced movement distance, maximum acceleration, and average velocity (P < 0.05) along with altered expression of neurodevelopment-related genes (gap43 and α1-tubulin) (P < 0.05). PS-MPs (25 µg/L, 250 µg/L; diameter at 25 µm) co-exposure not only significantly reduced the concentration of Pb in the exposed solution (P < 0.01), but also decreased the uptake of Pb by downregulating the divalent metal transporter 1 gene (dmt1) (P < 0.01), thereby alleviating Pb-induced neurotoxicity. However, to demonstrate that PS-MPs alleviate the neurotoxicity of Pb by reducing Pb uptake, upregulation of dmt1 by addition of deferoxamine (DFO, an efficient iron chelator, 100 µM) significantly increased the Pb uptake and exacerbated neurotoxicity in zebrafish. In summary, our results demonstrated that PS-MPs alleviate Pb neurotoxicity by downregulating the mRNA level of dmt1 and decreasing the Pb uptake. This study provides a new insight into the combined neurotoxicity and underlying mechanisms of PS-MPs and Pb on zebrafish.


Assuntos
Metais Pesados , Poluentes Químicos da Água , Animais , Poliestirenos/toxicidade , Poliestirenos/metabolismo , Microplásticos/toxicidade , Microplásticos/metabolismo , Plásticos/toxicidade , Peixe-Zebra/fisiologia , Chumbo/toxicidade , Chumbo/metabolismo , Larva/metabolismo , Metais Pesados/metabolismo , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/metabolismo
12.
Sci Total Environ ; 917: 170317, 2024 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-38301787

RESUMO

Lead (Pb), a pervasive and ancient toxic heavy metal, continues to pose significant neurological health risks, particularly in regions such as Southeast Asia. While previous research has primarily focused on the adverse effects of acute, high-level lead exposure on neurological systems, studies on the impacts of chronic, low-level exposure are less extensive, especially regarding the precise mechanisms linking ferroptosis - a novel type of neuron cell death - with cognitive impairment. This study aims to explore the potential effects of chronic low-level lead exposure on cognitive function and hippocampal neuronal ferroptosis. This research represents the first comprehensive investigation into the impact of chronic low-level lead exposure on hippocampal neuronal ferroptosis, spanning clinical settings, bioinformatic analyses, and experimental validation. Our findings reveal significant alterations in the expression of genes associated with iron metabolism and Nrf2-dependent ferroptosis following lead exposure, as evidenced by comparing gene expression in the peripheral blood of lead-acid battery workers and workers without lead exposure. Furthermore, our in vitro and in vivo experimental results strongly suggest that lead exposure may precipitate cognitive dysfunction and induce hippocampal neuronal ferroptosis. In conclusion, our study indicates that chronic low-level lead exposure may activate microglia, leading to the promotion of ferroptosis in hippocampal neurons.


Assuntos
Ferroptose , Chumbo , Humanos , Chumbo/toxicidade , Cognição , Aprendizado de Máquina , Biologia Computacional , Hipocampo , Neurônios
13.
Sci Total Environ ; 918: 170591, 2024 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-38309345

RESUMO

Microplastics (MPs), a new type of pollutant, have attracted much attention worldwide. MPs are often complexed with other pollutants such as heavy metals, resulting in combined toxicity to organisms in the environment. Studies on the combined toxicity of MPs and heavy metals have usually focused on the marine, while on the freshwater are lacking. In order to understand the combined toxic effects of MPs and heavy metals in the freshwater, five typical MPs (PVC, PE, PP, PS, PET) were selected to investigate the adsorption characteristics of MPs to Pb2+ before and after the MPs aging by ultraviolet (UV) irradiation through static adsorption tests. The results showed that UV aging enhanced adsorption of Pb2+ by MPs. It is noteworthy that MPs-PET had the highest adsorption capacity for Pb2+, and the interaction between MPs-PET and Pb2+ was the strongest. We specifically selected MPs-PET to study its combined toxicity with Pb2+ to Chlorella pyrenoidosa. In the combined toxicity test, MPs-PET and Pb2+ had significant toxic effects on Chlorella pyrenoidosa in the individual exposure, and the toxicity of individual Pb2+ exposure was greater than that of individual MPs-PET exposure. In the combined exposure, when MPs-PET and Pb2+ without adsorption (MPs-PET/Pb2+), MPs-PET and Pb2+ had a synergistic effect, which would produce strong physical and chemical stress on Chlorella pyrenoidosa simultaneously, and the toxic effect was the most significant. After the adsorption of MPs-PET and Pb2+ (MPs-PET@Pb2+), the concentration and activity of Pb2+ decreased due to the adsorption and fixation of MPs-PET, and the chemical stress on Chlorella pyrenoidosa was reduced, but the physical stress of MPs-PET still existed and posed a serious threat to the survival of Chlorella pyrenoidosa. This study has provided a theoretical basis for further assessment of the potential environmental risks of MPs in combination with other pollutants such as heavy metals.


Assuntos
Chlorella , Poluentes Ambientais , Metais Pesados , Poluentes Químicos da Água , Microplásticos/toxicidade , Plásticos/toxicidade , Chumbo/toxicidade , Poluentes Químicos da Água/análise , Metais Pesados/toxicidade , Metais Pesados/química , Adsorção
14.
Environ Int ; 184: 108479, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38340407

RESUMO

Lead is an environmentally widespread neurotoxic pollutant. Although the neurotoxicity of lead has been found to be closely associated with metabolic disorders, the effects of short-chain fatty acids on the neurotoxicity of lead and its mechanisms have not yet been explored. In this study, the results of open field tests and Morris water maze tests demonstrated that chronic lead exposure caused learning and memory deficits and anxiety-like symptoms in mice. The serum butyric acid content of lead-treated mice decreased in a dose-dependent manner, and oral administration of butyrate significantly improved cognitive memory impairment and anxiety symptoms in lead-exposed mice. Moreover, butyrate alleviated neuroinflammation caused by lead exposure by inhibiting the STAT3 signaling in microglia. Butyrate also promoted the expression of acetyl-CoA synthetase ACSS2 in hippocampal neurons, thereby increasing the content of acetyl-CoA and restoring the expression of both histone H3K9ac and the downstream BDNF. We also found that the median butyric acid concentration in high-lead exposure humans was remarkably lower than that in the low-lead exposure humans (45.16 µg/L vs. 60.92 µg/L, P < 0.01), and that butyric acid significantly mediated the relationship of lead exposure with the Montreal cognitive assessment scores, with a contribution rate of 27.57 %. In conclusion, our results suggest that butyrate supplementation is a possible therapeutic strategy for lead-induced neurotoxicity.


Assuntos
Fator Neurotrófico Derivado do Encéfalo , Doenças Neuroinflamatórias , Humanos , Camundongos , Animais , Ácido Butírico/uso terapêutico , Ácido Butírico/farmacologia , Acetilcoenzima A , Chumbo/toxicidade , Transtornos da Memória/induzido quimicamente , Cognição , Acetato-CoA Ligase
15.
Toxicology ; 503: 153760, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38387706

RESUMO

The health risks associated with exposure to heavy metals, such as Pb2+, are increasingly concerning the public. Pb2+ can cause significant harm to the human body through oxidative stress, autophagy, inflammation, and DNA damage, disrupting cellular homeostasis and ultimately leading to cell death. Among these mechanisms, apoptosis is considered crucial. It has been confirmed that transcription factors play a central role as mediators during the apoptosis process. Interestingly, these transcription factors have different effects on apoptosis depending on the concentration and duration of Pb2+ exposure. In this article, we systematically summarize the significant roles of several transcription factors in Pb2+-induced apoptosis. This information provides insights into therapeutic strategies and prognostic biomarkers for diseases related to Pb2+ exposure.


Assuntos
Chumbo , Fatores de Transcrição , Humanos , Chumbo/toxicidade , Fatores de Transcrição/genética , Apoptose , Morte Celular , Estresse Oxidativo
16.
Sci Total Environ ; 923: 170901, 2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38401722

RESUMO

Environmental and soil pollution increase the likelihood of human exposure to toxic metals. Therefore, there is a need for new methods and substances to protect individuals against the harmful effects caused by toxic metals. The study is the first to aim at determining the protective effect of cannabidiol (CBD) against oxidative stress and inflammation induced by toxic metal exposure in Transformed Human Liver Epithelial-2 (THLE-2) cell lines representing healthy liver cells. The IC50 value was determined by exposing THLE-2 human liver healthy cell line to different molarities of lead (Pb) using the XTT kit. The protective efficacy of CBD was assessed by adding 5 µM CBD in addition to the Pb doses determined at IC50 levels to the Pb groups created in cell lines. The levels of GSH, MDA, MPO, CAT, TNF-α, IL-1ß, and IL-6 in cell lines were determined using ELISA kits. The inhibition of toxic metal entry into the cells by CBD was assessed through ICP-MS analysis. The IC50 value for Pb was determined as 10 µM in 2D cell lines and 25 µM in 3D cell lines. It was observed that the application of 5 µM concentration of CBD, along with the determined IC50 doses for Pb, increased the cell proliferation rate. Furthermore, the decrease in GSH and CAT levels and the increase in MDA, MPO, TNF-α, IL-1ß, and IL-6 levels observed in cell lines treated only with Pb were reversed with the application of CBD. The ICP-MS analysis revealed that CBD reduced the cellular uptake of Pb. The reversal of oxidative stress and inflammation induced by Pb, the increase in cell proliferation, and the reduction in the cellular uptake of toxic metals by CBD can be considered as strong evidence for the protective use of CBD in Pb exposures.


Assuntos
Canabidiol , Humanos , Canabidiol/toxicidade , Fator de Necrose Tumoral alfa , Interleucina-6 , Chumbo/toxicidade , Fígado , Inflamação/induzido quimicamente , Linhagem Celular
17.
Chemosphere ; 352: 141414, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38336042

RESUMO

The use of silicon fertilizer (SF) as a means of remediating cadmium (Cd) and lead (Pb) pollution has proven to be beneficial. However, the mechanism via which SF enhances soil quality and crop productivity under Cd- and Pb-contaminated soil (S) remains unclear. This study investigated the impacts of chemical fertilizer, mineral SF (MSF), and organic SF (OSF) on microbial community structure, activity of nutrient acquisition enzymes, and growth of tobacco in the presence of S condition. SF significantly reduced the contents of Cd and Pb in soil under S condition by 6.92-42.43% and increased plant height and leaf area by 15.27-81.77%. Moreover, the use of SF was observed to increase the efficiency of soil carbon and phosphorus cycling under S condition by 6.88-23.08%. Concurrently, SF was found to play a crucial role in facilitating the establishment of a complex, efficient, and interdependent molecular ecological network among soil microorganisms. In this context, Actinobacteriota, Bacteroidota, Ascomycota, and Basidiomycota were observed to be integral components of this network. SF was found to have a substantial positive impact on the metabolic functions and organismal systems of soil microorganisms. Moreover, the combined utilization of the Mantel test and partial least squares path model provided empirical evidence supporting the assertion that the administration of SF had a positive impact on both soil nutrient acquisition enzyme activity and tobacco growth, which was attributed to the enhancement of soil microbial diversity resulting from the application of SF. Furthermore, compared with MSF, OSF has advantages in reducing soil Pb and Cd content, promoting tobacco agronomic traits, increasing the number of key microbial communities, and maintaining the structural stability of microbial networks. The aforementioned findings, therefore, suggest that the OSF played a pivotal role in alleviating the adverse impacts of S, thereby demonstrating its efficacy in this particular process.


Assuntos
Metais Pesados , Poluentes do Solo , Cádmio/análise , Fertilizantes , Silício , Chumbo/toxicidade , Solo/química , Microbiologia do Solo , Metais Pesados/análise , Poluentes do Solo/análise
18.
J Environ Manage ; 354: 120153, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38394868

RESUMO

Aggravated metal pollution in wetland and riparian zones has become a global environmental issue, necessitating the identification of sustainable remediation approaches. Salix exhibits great potential as a viable candidate for metal(loid) remediation. However, the underlying mechanisms for its effectiveness in different flooding regimes with Pb pollution have not been extensively studied. In this study, fast-growing Salix×jiangsuensis 'J172' was selected and planted in different Pb polluted soils (control, 400 and 800 mg ∙ kg-1) under non-flooded and flooded (CF: continuous flooding and IF: intermittent flooding) conditions for 60 days. This study aimed to explore the effects of flooding on Salix growth performance, physiological traits, and the relationship between Pb uptake/translocation and root Fe plaques. Salix×jiangsuensis 'J172' exhibited excellent tolerance and adaptation to Pb pollution with a tolerance index (TI) exceeding 0.6, even at the highest Pb levels. Moreover, the TIs under flooded conditions were higher than that under non-flooded conditions, suggesting that flooding could alleviate Pb toxicity under co-exposure to Pb and flooding. Leaf malondialdehyde (MDA) exhibited a dose-dependent response to Pb exposure; however, CF or IF mitigated the oxidative damage induced by Pb toxicity with decreased MDA content (2.2-11.9%). The superoxide dismutase and peroxidase activities were generally enhanced by flooding, but combined stress (flooding and Pb) significantly decreased catalase activity. Pb was predominantly accumulated in Salix roots, and flooding markedly increased root Pb accumulation by 19.2-173.0% compared to non-flooded condition. Additionally, a significant positive correlation was observed between the iron (Fe) content of the root plaque and root Pb accumulation, indicating that the formation of Fe plaque on the root surface could enhance the phytostabilization of Pb in Salix. The current findings highlight that fast-growing woody plants are suitable for phyto-management of metal-polluted wetlands and can potentially minimize the risk of metal mobility in soils.


Assuntos
Salix , Poluentes do Solo , Ferro , Chumbo/toxicidade , Biodegradação Ambiental , Antioxidantes/farmacologia , Plantas , Solo , Poluentes do Solo/análise , Raízes de Plantas/química
19.
Chemosphere ; 352: 141521, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38395370

RESUMO

Contamination by potentially toxic metals and metalloids (PTMs) has become a significant health and environmental issue worldwide. Sorption has emerged as one of the most prominent strategies for remediating both soil and water contamination. New sorbents are being developed to provide economically viable and environmentally sound alternatives, in alignment with the principles of the Sustainable Development Goals. This research aimed to assess the potential effects on human health and environmental toxicity following the sorption of cadmium (Cd), lead (Pb), and zinc (Zn) using peat, compost, and biochar as sorbents. The peat was collected in Brazil, a country with a tropical climate, while the compost and biochar were produced from the organic fraction of municipal solid waste (OFMSW). In terms of bioaccessibility, the results showed the following order: compost < biochar < peat for Pb, and compost < peat < biochar for Cd and Zn. There was a significant growth inhibition for Eruca sativa and Zea mays exposed to increasing concentrations of PTMs treated with peat and compost. The presence of contaminants played a decisive role on immobilization of neonates of Ceriodaphnia silvestrii after treatments with compost and, especially, peat. However, the biochar addition rate caused a significant influence on the outcomes of ecotoxicity across all tested species. Although the samples treated with biochar exhibited lower residual concentrations of PTMs than those treated with compost and peat, the inherent toxicity of biochar might be attributed to the material itself. The exposure to residual PTM concentrations post-desorption caused ecotoxic effects on tested species, emphasizing the need to assess PTM desorption potential. Peat, compost, and biochar are promising alternatives for the sorption of PTMs, but the addition rates must be properly adjusted to avoid the occurrence of undesirable ecotoxicological effects. This research offers valuable insights for sustainable environmental management and protection by thoroughly investigating the impacts of different sorbents and contaminants on aquatic and terrestrial ecosystems.


Assuntos
Compostagem , Metais Pesados , Poluentes do Solo , Humanos , Recém-Nascido , Cádmio/toxicidade , Solo , Chumbo/toxicidade , Ecossistema , Poluentes do Solo/toxicidade , Poluentes do Solo/análise , Carvão Vegetal , Zinco/toxicidade , Metais Pesados/análise
20.
Food Funct ; 15(6): 3060-3075, 2024 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-38414441

RESUMO

Lead (Pb) is a widespread toxic endocrine disruptor that could cause liver damage and gut microbiota dysbiosis. However, the causal relationship and underlying mechanisms between the gut microbiota and Pb-induced liver injury are unclear. In this study, we investigated the metabolic toxicity caused by Pb exposure in normal chow (Chow) and high-fat diet (HFD) mice and confirmed the causal relationship by fecal microbial transplantation (FMT) and antibiotic cocktail experiments. The results showed that Pb exposure exacerbated HFD-induced hepatic lipid deposition, fibrosis, and inflammation, but it had no significant effect on Chow mice. Pb increased serum lipopolysaccharide (LPS) levels and induced intestinal inflammation and barrier damage by activating TLR4/NFκB/MLCK in HFD mice. Furthermore, Pb exposure disrupted the gut microbiota, reduced short-chain fatty acid (SCFA) concentrations and the colonic SCFA receptors, G protein-coupled receptor (GPR) 41/43/109A, in HFD mice. Additionally, Pb significantly inhibited the hepatic GPR109A-mediated adenosine 5'-monophosphate-activated protein kinase (AMPK) pathway, resulting in hepatic lipid accumulation. FMT from Pb-exposed HFD mice exacerbated liver damage, disturbed lipid metabolic pathways, impaired intestinal barriers, and altered the gut microbiota and metabolites in recipient mice. However, mice exposed to HFD + Pb and HFD mice had similar levels of these biomarkers in microbiota depleted by antibiotics. In conclusion, our study provides new insights into gut microbiota dysbiosis as a potential novel mechanism for human health related to liver function impairment caused by Pb exposure.


Assuntos
Microbioma Gastrointestinal , Humanos , Animais , Camundongos , Chumbo/toxicidade , Dieta Hiperlipídica/efeitos adversos , Disbiose , Inflamação , Antibacterianos , Lipopolissacarídeos , Fígado
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