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1.
Acta Trop ; 237: 106748, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36368413

RESUMO

Giardiasis is an intestinal protozoal disease caused by Giardia lamblia (G. lamblia) which is a major worldwide health problem due to development of resistance to commonly used drugs. Therefore, it is necessary to identify an effective drug for giardiasis. This study aimed to assess the therapeutic role of L-citrulline against giardiasis in experimental animals. 40 male Swiss Albino weaned rats were used in this study, divided into four groups. Group I: normal control; group II: infected un-treated; group III: infected and treated with L-citrulline and Group IV: infected and treated with metronidazole. The efficacy was evaluated by counting Giardia trophozoites in the intestinal mucosa and cysts in the stool of infected rats. Histopathological analyses, immunohistochemistry expression of inducible nitric oxide synthase (iNOS) in the small intestine tissues were performed. Along with, serum IL6, the intestinal arginase enzyme level and giardial flavohemoglobin (flavoHb) expression were measured. L-citrulline administration reduced the mean number of G. lamblia cysts and trophozoites, serum IL-6, and intestinal arginase enzyme levels. Furthermore, the intestinal brush border was restored, with a reduction in the inflammatory infiltrate and an increase in iNOS activity. Moreover, there was a significant decrease in flavoHb gene expression in both the L-citrulline and metronidazole treated groups. Thus L-citrulline is effective in NO production therefore it has a therapeutic potential in controlling giardiasis.


Assuntos
Cistos , Giardia lamblia , Giardíase , Masculino , Camundongos , Ratos , Animais , Giardíase/tratamento farmacológico , Citrulina/farmacologia , Citrulina/uso terapêutico , Metronidazol/farmacologia , Metronidazol/uso terapêutico , Arginase , Giardia , Trofozoítos , Arginina/farmacologia , Imunidade
2.
J Med Case Rep ; 16(1): 397, 2022 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-36316785

RESUMO

BACKGROUND: Lymphangiomas are rare benign malformations of the lymphatics that occur due to blockage of the lymphatic system during fetal development. They commonly occur in the neck and axilla, while involvement of the pericardium is rare. We report herein the case of a 16-month-old Sri Lankan child with a large pericardial cystic lymphangioma presenting with sudden-onset shortness of breath. CASE PRESENTATION: A 16-month-old Sri Lankan boy presented with sudden-onset dyspnea for 1-day duration following a febrile illness that lasted 2 days. On examination, he was afebrile and had subcostal, intercostal, and suprasternal recessions, with a respiratory rate of 50 breaths per minute. He had a loud expiratory grunt. The chest expansion was reduced on the right side, which was dull to percussion. Auscultation revealed a marked reduction of air entry over the right lower and mid zones. Chest X-ray showed a well-demarcated opacity involving the lower and mid zones of the right hemithorax associated with a tracheal shift to the opposite side. Ultrasound scan of the chest revealed fluid-filled right hemithorax suggesting a septate pleural effusion. A contrast-enhanced computed tomography scan of the thorax showed a large multiloculated extrapulmonary cystic lesion involving the right hemithorax with a mediastinal shift towards the left side associated with displacement of the right-side mediastinal structures. He underwent mini-thoracotomy and surgical excision of the cyst. A large cyst originating from the pericardium was observed and excised during surgery. Histological examination revealed a lesion composed of cysts devoid of a lining epithelium but separated by connective tissue, mature adipose tissue, and lymphoid aggregates. The child showed complete recovery postoperatively with full expansion of the ipsilateral lung. CONCLUSION: We report the case of a patient with cystic lymphangioma who was perfectly well and asymptomatic until 16 months of age. This case report presents the very rare occurrence of a large cystic lymphangioma originating from the pericardium. It highlights the importance of considering rare possibilities and performing prompt imaging in situations of diagnostic uncertainty to arrive at an accurate diagnosis that can be lifesaving.


Assuntos
Cistos , Linfangioma Cístico , Linfangioma , Neoplasias do Mediastino , Síndrome do Desconforto Respiratório , Masculino , Criança , Humanos , Lactente , Linfangioma Cístico/diagnóstico , Linfangioma Cístico/diagnóstico por imagem , Neoplasias do Mediastino/diagnóstico , Linfangioma/complicações , Linfangioma/cirurgia , Pericárdio/diagnóstico por imagem , Pericárdio/patologia , Dispneia , Cistos/complicações
3.
Molecules ; 27(21)2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36364003

RESUMO

The current study examines the desiccation-resistant Ramlibacter tataouinensis TTB310T as a model organism for the production of novel exopolysaccharides and their structural features. This bacterium is able to produce dividing forms of cysts which synthesize cell-bound exopolysaccharide. Initial experiments were conducted on the enrichment of cyst biomass for exopolysaccharide production under batch-fed conditions in a pilot-scale bioreactor, with lactate as the source of carbon and energy. The optimized medium produced significant quantities of exopolysaccharide in a single growth phase, since the production of exopolysaccharide took place during the division of the cysts. The exopolysaccharide layer was extracted from the cysts using a modified trichloroacetic acid method. The biochemical characterization of purified exopolysaccharide was performed by gas chromatography, ultrahigh-resolution mass spectrometry, nuclear magnetic resonance, and Fourier-transform infrared spectrometry. The repeating unit of exopolysaccharide was a decasaccharide consisting of ribose, glucose, rhamnose, galactose, mannose, and glucuronic acid with the ratio 3:2:2:1:1:1, and additional substituents such as acetyl, succinyl, and methyl moieties were also observed as a part of the exopolysaccharide structure. This study contributes to a fundamental understanding of the novel structural features of exopolysaccharide from a dividing form of cysts, and, further, results can be used to study its rheological properties for various industrial applications.


Assuntos
Comamonadaceae , Cistos , Humanos , Cromatografia Gasosa-Espectrometria de Massas , Ramnose , Polissacarídeos Bacterianos/química
4.
BMJ Case Rep ; 15(11)2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36357099

RESUMO

Haematospermia, even though alarming, is usually benign and self-limiting, especially in a sexually active male. Nevertheless recurrent, refractory or painful haematospermia is troublesome and warrants thorough evaluation. In this context, we describe a rare case of recurrent haematospermia whereby evaluation revealed haemorrhage in seminal vesicle cysts and consequently established the aetiology of autosomal dominant polycystic kidney disease.


Assuntos
Cistos , Doenças dos Genitais Masculinos , Hemospermia , Rim Policístico Autossômico Dominante , Masculino , Humanos , Hemospermia/complicações , Rim Policístico Autossômico Dominante/complicações , Doenças dos Genitais Masculinos/etiologia , Cistos/etiologia
5.
Arch Argent Pediatr ; 120(6): e272-e277, 2022 12.
Artigo em Espanhol | MEDLINE | ID: mdl-36374065

RESUMO

Numerous entities in the pediatric population can present in the form of cysts or as lesions with similar characteristics. Of the pathologies that can cause these images in children, infectious diseases are the most frequent. We present the case of a native of Bolivia with recent immigration to Argentina who presented a pulmonary co-infection with tuberculosis and hydatidosis. Both infections can present with similar signs and symptoms and although this association is rarely reported in the literature, certain immunological mechanisms could intervene in the causal association of co-infection between helminth parasites and mycobacteria. Both pathologies are very prevalent infections in our region and should be taken into account among the differential diagnoses in patients with cystic or cavitary pulmonary diseases.


Existen numerosas entidades en la población pediátrica que pueden presentarse en forma de quistes o como lesiones de similares características. De estas patologías, las infecciosas son las más frecuentes. Se presenta el caso de una paciente oriunda de Bolivia con migración reciente a la Argentina que presentó una coinfección con tuberculosis e hidatidosis pulmonar. Ambas infecciones se pueden presentar con signos y síntomas similares y, aunque la asociación citada es poco frecuente en la bibliografía, ciertos mecanismos inmunitarios podrían intervenir en la coinfección de parásitos helmintos y micobacterias. Ambas patologías son infecciones prevalentes en nuestra región y deben ser tenidas en cuenta entre los diagnósticos diferenciales ante pacientes con imágenes quísticas o cavitarias pulmonares.


Assuntos
Coinfecção , Cistos , Equinococose , Pneumopatias , Tuberculose , Humanos , Criança , Coinfecção/diagnóstico , Equinococose/diagnóstico , Tuberculose/complicações , Tuberculose/diagnóstico
6.
BMC Surg ; 22(1): 381, 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36336689

RESUMO

BACKGROUND: Gastric duplication cyst associated with ectopic pancreas is rare and we aimed to alert clinician to this congenital anomaly. CASE PRESENTATION: A 15-year-old girl presented with intermittent vomiting. Gastroscopy showed a submucosal tumor with an approximate diameter of 40 mm in the anterior wall of the gastric antrum. The lesion had a central umbilication and was diagnosed preliminarily as gastric ectopic pancreas with pseudocyst formation on the basis of its appearance. However, computed tomographic scan showed a thick-walled cystic lesion with an enhanced outline of the cystic wall in the antrum of stomach, suggestive of duplication cyst. Serum amylase was normal. Endoscopic ultrasonography revealed a solid-cystic lesion; the solid portion were inhomogeneously mixed with echoes, and had indistinct border to muscularis propria; the cystic portion had echogenic internal mucosal layer and distinct border to muscularis propria. Endoscopic submucosal dissection (ESD) was suggested for the patient to relieve symptoms and diagnose the lesion definitely. The operation procedure was uneventful and the solid-cystic lesion was resected completely. Histopathologic examination revealed that the solid portion was ectopic pancreas, and the cystic portion was gastric duplication cyst. After resection, the patient discharged successfully and neither symptoms nor tumors recurred during the 9 months follow-up period. CONCLUSIONS: This is the first case of a solid-cystic lesion with central umbilication in the stomach diagnosed as gastric duplication cyst associated with ectopic pancreas. ESD could be an optional treatment to provide a definitive diagnosis.


Assuntos
Cistos , Ressecção Endoscópica de Mucosa , Enteropatias , Neoplasias Gástricas , Feminino , Adolescente , Humanos , Ressecção Endoscópica de Mucosa/métodos , Recidiva Local de Neoplasia/patologia , Gastroscopia/métodos , Pâncreas/cirurgia , Pâncreas/patologia , Cistos/diagnóstico , Cistos/cirurgia , Enteropatias/patologia , Neoplasias Gástricas/cirurgia , Mucosa Gástrica/cirurgia , Mucosa Gástrica/patologia
7.
Urologiia ; (5): 84-89, 2022 Nov.
Artigo em Russo | MEDLINE | ID: mdl-36382823

RESUMO

INTRODUCTION: Retrograde intrarenal surgery (RIRS) is being actively implemented in the treatment of renal stones and other diseases. If necessary, RIRS can be combined with percutaneous procedures. AIM: To study the results of RIRS in patients with nephrolithiasis and various renal disorders. MATERIALS AND METHODS: A total of 106 patients who undergone RIRS were included in the study. There were 66 men (63.4%) and 40 women (36.6%). Mean age was 46.8+/-15.6 years. The indication for RIRS in 84 (79.2%) patients was renal stones. Calculus in calyceal diverticulum were diagnosed in 6 (5.7%), encrusted stents in 8 (7.7%), urinary fistulas after partial nephrectomy in 5 (4.7%), pelvis tumors in 2 (1.9%), pelvis perforation after marsupialization of parapelvic cyst in 1 (0.9%) patient, respectively. Simultaneous retro- and antegrade procedures were performed in 27 (25.5%) cases. In those with nephrolithiasis and encrusted stents, lithotripsy was done with the removal of stone fragments. Two patients underwent endoscopic resection of the pelvis tumor. In six patients, the neck of the diverticulum was incised after lithotripsy, while in five cases retrograde endoscopically controlled percutaneous treatment urinary fistulae was performed. In one case, laser fulguration of the pelvis defect with stenting was done. RESULTS: RIRS was effective in 72 (85.7%) of 84 patients with renal stones. The operation time was 70.8+/-10.2 minutes. In 12 (14.3%) cases with residual fragments, extracorporeal shock-wave lithotripsy (n=7) and repeated RIRS (n=5) were performed. The efficiency of RIRS after two sessions was 91.7%. Complications were observed in 11 (10.4%) patients. With encrusted stents, the operation time was 95.0+/-16.5 min. After laser fragmentation of encrustations in the lower part of the stent, percutaneous lithotripsy was performed with antegrade removal of its upper half. The time for RIRS in those with diverticula was 60.0+/-8.5 min, the average stone size was 8 mm (from 6 to 10 mm). In all cases lithotripsy with mucosal fulguration was successfully done. The procedures for urinary fistulae were also effective (operation time was 45.0 +/- 20.5 minutes) and there were no complications. CONCLUSION: RIRS is a safe and effective treatment for patients with nephrolithiasis and other kidney disorders. Modern flexible ureteroscopes allows to examine the collecting system and to perform lithotripsy and removal of stone fragments.


Assuntos
Cistos , Divertículo , Cálculos Renais , Litotripsia , Nefrolitotomia Percutânea , Fístula Urinária , Masculino , Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Litotripsia/métodos , Cálculos Renais/terapia , Nefrolitotomia Percutânea/métodos , Resultado do Tratamento , Divertículo/etiologia
8.
BMC Ophthalmol ; 22(1): 432, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36376819

RESUMO

BACKGROUND: The developmental pathways and subsequent evolutional processes of idiopathic lamellar macular hole (LMH) were studied with spectrum domain optical coherence tomography (SD-OCT). METHODS: Twenty-seven eyes of 26 patients of idiopathic LMH with pre-LMH SD-OCT available were retrospectively reviewed. Relevant OCT parameters and best-corrected visual acuity (BCVA) were collected and analyzed. RESULTS: Four types of developmental pathways of idiopathic LMH were noted. Type 1 (5 cases), involved disruption of a foveal cyst from vitreomacular traction. Type 2 (10 cases), demonstrated rupture of parafoveal cysts or schisis mainly from epiretinal membrane (ERM). In type 3 pathway (5 cases), a central intraretinal cyst formed under tight ERM with subsequent cyst roof dehiscence. Type 4 (7 cases), showed gradual loss of foveal tissue without cystic lesions from ERM traction. There was no statistically significant change in BCVA during LMH formations or subsequent evolutional processes in any types of the developmental pathways. Three cases developed epiretinal proliferation (EP) during evolution, which showed tendency of decrease in BCVA. Among the three cases, one later developed the degenerative configuration. CONCLUSIONS: In summary, four types of tractional developmental pathways of idiopathic LMH were identified. BCVA was relatively stable during LMH formation and follow-up. Deterioration of visual acuity were found in cases that developed EP during evolution. Transformation into degenerative configuration might be possible after LMH formation.


Assuntos
Cistos , Membrana Epirretiniana , Perfurações Retinianas , Humanos , Perfurações Retinianas/cirurgia , Projetos Piloto , Estudos Retrospectivos , Seguimentos , Membrana Epirretiniana/diagnóstico , Tomografia de Coerência Óptica/métodos , Vitrectomia/métodos
9.
Int J Paleopathol ; 39: 109-114, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36347173

RESUMO

OBJECTIVE: To undertake differential diagnosis of scapular bone defects found in two medieval skeletons buried in different necropoles of Friuli Venezia Giulia (Italy) and to evaluate the clinical significance of paralabral cysts in the context of shoulder pathology. MATERIALS: Individual JoT36 is an adult male exhumed from a necropolis belonging to a rural agricultural settlement dated to the 10th to 11th century A.D. Individual CIVT58 is an adult male from a Langobard necropolis dated to 630-670 A.D. METHODS: Macroscopic examination of the skeletons was performed using standard osteological methods and review of pertinent clinical literature to assist differential diagnosis. RESULTS: Between the glenoid cavity and the spinoglenoid notch of the right scapula of JoT36 there is a multilocular circular defect adjacent to the glenoid rim. On the neck of the left scapula of CIVT58, immediately above the spinoglenoid notch, there is a unilocular circular depression. In both cases, the cortical bone appears smooth without any evidence of erosion or sclerosis. CONCLUSIONS: Skeletal findings and historical/archaeological contexts of both cases are compatible with the diagnosis of paralabral cysts. SIGNIFICANCE: Paralabral cysts are relatively frequently observed in clinical settings but very few examples have been documented in paleopathological literature. This study seeks to improve recognition and interpretation of this pathology in historical/archaeological contexts. LIMITATIONS: Findings from a case report can neither generate epidemiological information nor be generalized. SUGGESTIONS FOR FURTHER RESEARCH: Identification of new cases may add valuable information about lifestyles and related shoulder pathologies in ancient times.


Assuntos
Cistos , Ombro , Masculino , Adulto , Humanos , Paleopatologia , Escápula , Arqueologia
10.
Dtsch Arztebl Int ; 119(31-32): 534, 2022 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-36384921
11.
Medicine (Baltimore) ; 101(45): e31756, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36397328

RESUMO

INTRODUCTION: Postoperative discal pseudocyst (PDP) is rare. Few studies have reported on the recurrence of PDPs, and there is a lack of understanding of their clinical features and treatment methods. This study discusses the clinical characteristics and treatment of recurrent PDPs. PATIENT CONCERNS: A 25-year-old man presented with radiating pain and numbness in the lateral left calf and dorsum of the foot. DIAGNOSIS: Postoperative discal pseudocyst. INTERVENTIONS: He underwent lumbar discectomy, which provided immediate postoperative relief. However, the symptoms recurred 45 days later. Magnetic resonance imaging (MRI) showed a lesion compressing the dura and nerve roots at the site of the previous surgery. The lesion appeared hypointense on T1-weighted imaging and hyperintense on T2-weighted imaging. The patient was treated conservatively for 1 month without significant relief. He then underwent lumbar discectomy and cyst removal, which immediately relieved his symptoms. However, 27 days later, the patient again developed the same symptoms. MRI examination showed recurrence of PDP. As 1 month of conservative treatment failed to relieve the patient's symptoms, we performed posterior instrumented lumbar fusion and cyst removal. OUTCOMES: The patient's symptoms disappeared, and have not recurred for 1 year at the time of writing. CONCLUSIONS: PDP is a rare complication of lumbar discectomy. Repeat lumbar discectomy can effectively treat PDP, but the cyst can recur. We, for the first time, used posterior instrumented lumbar fusion to successfully treat recurrent PDP.


Assuntos
Cistos , Vértebras Lombares , Masculino , Humanos , Adulto , Vértebras Lombares/cirurgia , Vértebras Lombares/patologia , Cistos/etiologia , Cistos/cirurgia , Cistos/diagnóstico , Discotomia/efeitos adversos , Período Pós-Operatório , Dura-Máter/patologia
12.
Cir Cir ; 90(5): 697-699, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36327478

RESUMO

OBJECTIVE: Presentation of a case little reported in the literature of Fitz-Hugh-Curtis Syndrome regarding gender and its association with simple intra-abdominal mesothelial cyst. METHOD: A bibliographic source was compiled regarding the unusual presentation of the syndrome in males and its association with the simple mesothelial cyst. RESULTS: The affectation in men is infrequent, with only 10 reported cases. The simple peritoneal mesothelial cyst has an approximate incidence of 1/1,000,000. CONCLUSIONS: We are faced with an unusual presentation in terms of pathology and association, so it is important to carry out the case report.


OBJETIVO: Presentar un caso, poco reportado en la literatura, de síndrome de Fitz-Hugh-Curtis en cuanto al sexo y su asociación con quiste mesotelial simple intraabdominal. MÉTODO: Se realizó la recopilación de fuentes bibliográficas respecto a la presentación inusual del síndrome en el sexo masculino y su asociación con el quiste mesotelial simple. RESULTADOS: La afectación en varones es infrecuente, con solo 10 casos reportados. El quiste mesotelial simple peritoneal tiene una incidencia aproximada de 1/1,000,000. CONCLUSIONES: Nos encontramos ante una presentación inusual en cuanto a patología y asociación, por lo que es de relevancia realizar el reporte del caso.


Assuntos
Infecções por Chlamydia , Cistos , Hepatite , Doença Inflamatória Pélvica , Peritonite , Masculino , Feminino , Humanos , Infecções por Chlamydia/complicações , Peritonite/complicações
13.
Laryngorhinootologie ; 101(11): 929-931, 2022 11.
Artigo em Alemão | MEDLINE | ID: mdl-36328057

Assuntos
Cistos , Boca , Humanos , Orofaringe
14.
Surg Clin North Am ; 102(6): 1089-1102, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36335927

RESUMO

Cystic conditions are the most common disorder of the breast. Simple cysts are not malignant and do not require intervention. Patients with symptomatic simple cysts can undergo elective aspiration, and typical cyst fluid can be discarded. Bloody fluid should be sent for cytology. Cysts with thick walls, thick septations, or solid components have a risk of malignancy and should undergo biopsy.


Assuntos
Cistos , Humanos , Cistos/diagnóstico , Cistos/terapia , Biópsia
16.
Sultan Qaboos Univ Med J ; 22(4): 583-586, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36407698

RESUMO

Hydatid cyst (HC) disease is a parasitic infection produced by cysts containing the Echinococcus granulosus larval phase. Patients with HC disease are typically asymptomatic until incidentally diagnosed or when complications occur. A rare presentation of liver HC is spontaneous cutaneous fistualisation. We report a 63-year-old female patient admitted in a tertiary care hospital in Ibb, Yemen, in 2019 with an infected cutaneous fistula induced by a ruptured HC. The patient underwent laparotomy and partial cystectomy with excision of the fistula tract. Upon 6-month follow-up, there was no HC recurrence. This report highlights the need for physicians to consider this diagnosis when faced with an unusual cutaneous fistula near organs commonly involved in HC, especially in areas where the prevalence of this disease is high.


Assuntos
Fístula Cutânea , Cistos , Equinococose Hepática , Equinococose , Feminino , Humanos , Pessoa de Meia-Idade , Fístula Cutânea/parasitologia , Fístula Cutânea/cirurgia , Equinococose/complicações , Equinococose/cirurgia , Equinococose Hepática/complicações , Equinococose Hepática/diagnóstico , Equinococose Hepática/cirurgia
17.
Ann Intern Med ; 175(10): 1489, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36252249
18.
BMC Surg ; 22(1): 369, 2022 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-36309680

RESUMO

OBJECTIVE: To investigate the efficacy and clinical application advantage of omental tamponade with vascular pedicle combined with Laparoscopic fenestration for the treatment of diaphragmatic hepatic cyst. METHODS: A total of 56 patients with diaphragmatic hepatic cysts underwent laparoscopic surgery in a single tertiary academic medical center from January 2010 to October 2020, including 21 patients (non-omental group) underwent laparoscopic fenestration of liver cysts, and 36 patients underwent laparoscopic liver cyst fenestration combined with vascular pedicle omentum tamponade (omental group). The general conditions and follow-up results of the two groups were compared and annalyzed. RESULTS: The operation time of the omental group was longer than that of the non-omental group (P = 1.358E-4). There was no significant difference in postoperative complications, postoperative laboratory values and hospital costs (P>0.05). The length of hospital stay in omental group was shorter than that in non-omental group (P = 0.034). In the omental group, recurrence occurred in 1 of 35 patients (4.65%) who were followeded up 12 months after surgery. In the non-omental group, of the 21 patients followed, 3 patients (14.28%) recurred 6 months after surgery, and 8 patients (38.10%) recurred 12 months after surgery. CONCLUSION: It is an effective method to prevent the recurrence of diaphragmatic hepatic cyst after laparoscopic fenestration by packing the cyst with vascularized omentum.


Assuntos
Cistos , Laparoscopia , Hepatopatias , Doenças Torácicas , Humanos , Omento/cirurgia , Cistos/cirurgia , Hepatopatias/cirurgia , Laparoscopia/métodos , Fígado , Doenças Torácicas/cirurgia
19.
Parasite ; 29: 49, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36315102

RESUMO

Giardiosis is a worldwide intestinal parasitosis, affecting both humans and animals. Treatment in dogs remains limited and the lack of efficacy of the few approved medications is a rising concern. In this study, 23 dogs raised by veterinary students and naturally infected with Giardia duodenalis were treated in home conditions with fenbendazole (50 mg/kg orally for 5 consecutive days). Fecal samples were collected immediately before treatment (FS1), 2-4 days after treatment (FS2) and 8-10 days after treatment (FS3). Giardia duodenalis cyst excretion was measured quantitatively by direct immunofluorescence assay (DFA) at FS1, FS2 and FS3. Molecular typing with a nested PCR targeting the SSU rDNA locus was also performed at FS1 and FS2. Fecal consistency improved in 16/21 dogs (76%) and mean cyst shedding was reduced by 84% after treatment. However, only 8/23 dogs (35%) achieved therapeutic success (≥90% reduction of cysts) and only 4/23 dogs (17%) had complete elimination of G. duodenalis. Molecular typing showed that dogs harbored only canine-specific assemblages, with a high prevalence of assemblage C in analyzed samples (30/39). We also detected different assemblages after treatment and nucleotide substitutions in assemblage C sequences that have not been described previously. Eight to ten days after treatment, high Giardia cyst excretion was measured, suggesting possible reinfection despite hygiene measures and/or multiplication. These data suggest that fenbendazole treatment may improve fecal consistency but has limited therapeutic efficacy against giardiosis in this population of dogs. Further research is still needed to assess the efficacy of fenbendazole against canine giardiosis.


Title: Absence d'efficacité du fenbendazole contre Giardia duodenalis dans une population de chiens naturellement infectés en France. Abstract: La giardiose est une parasitose intestinale mondiale, touchant à la fois l'homme et les animaux. Chez le chien, le traitement reste limité et le manque d'efficacité des quelques médicaments autorisés inquiète de plus en plus. Dans cette étude, 23 chiens d'étudiants vétérinaires et infectés naturellement par Giardia duodenalis ont été traités en conditions réelles avec du fenbendazole (50 mg/kg par voie orale pendant 5 jours consécutifs). Des échantillons de selles ont été collectés juste avant le traitement (FS1), 2­4 jours après traitement (FS2) et 8­10 jours après traitement (FS3). L'excrétion de kystes de G. duodenalis a été mesurée quantitativement par immunofluorescence directe (IFD) à FS1, FS2 et FS3. Un génotypage par PCR nichée ciblant le locus SSU ADNr a également été réalisé à FS1 et FS2. La consistance des selles a été améliorée chez 16/21 (76 %) chiens et la moyenne d'excrétion des kystes a été réduite de 84 % juste après le traitement. Seulement 8/23 (35 %) chiens ont atteint un succès thérapeutique (≥ 90 % de réduction d'excrétion de kystes) et 4/23 (17 %) chiens ont eu une élimination complète de G. duodenalis. L'analyse des séquences a montré que les chiens présentaient seulement des assemblages génotypiques spécifiques de l'espèce canine, avec une forte prévalence de l'assemblage C dans les échantillons analysés (30/39). Des changements d'assemblage après traitement et des substitutions nucléotidiques jamais décrites au sein de l'assemblage C ont également été observés. Huit à dix jours après traitement, une forte excrétion de kystes de G. duodenalis a été mesurée : malgré les mesures hygiéniques, une réinfection et/ou une multiplication semblent probables. Ces données suggèrent que le traitement au fenbendazole peut améliorer la consistance des selles mais a une efficacité thérapeutique limitée contre la giardiose dans cette population de chiens. Des recherches supplémentaires sont encore nécessaires pour évaluer l'efficacité du fenbendazole contre la giardiose canine.


Assuntos
Cryptosporidium , Cistos , Doenças do Cão , Giardia lamblia , Giardíase , Humanos , Cães , Animais , Giardia lamblia/genética , Fenbendazol/uso terapêutico , Giardia/genética , Doenças do Cão/tratamento farmacológico , Doenças do Cão/epidemiologia , Oocistos , Giardíase/tratamento farmacológico , Giardíase/veterinária , Giardíase/epidemiologia , Fezes , Cistos/tratamento farmacológico , Genótipo
20.
Int J Mol Sci ; 23(20)2022 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-36293397

RESUMO

Increased DNA damage response (DDR) signaling in kidney cyst-lining epithelial cells (CECs) may provide an opportunity for cell-specific therapeutic targeting in autosomal dominant polycystic kidney disease (ADPKD). We hypothesized that inhibiting ataxia telangiectasia mutated (ATM; a proximal DDR kinase) together with low-dose cisplatin overwhelms the DDR response and leads to selective apoptosis of cyst-lining epithelial cells (CECs). Pkd1RC/RC/Atm+/- mice were treated with either vehicle or a single low-dose cisplatin, and the acute effects on CECs (DNA damage and apoptosis) after 72 h and chronic effects on progression (cyst size, inflammation, fibrosis) after 3 weeks were investigated. At 72 h, cisplatin caused a dose-dependent increase in γH2AX-positive nuclei in both CECs and non-cystic tubules but did not cause selective apoptosis in Pkd1RC/RC/Atm+/- mice. Moreover, the increase in γH2AX-positive nuclei was 1.7-fold lower in CECs compared to non-cystic epithelial cells (p < 0.05). Low-dose cisplatin also did not alter long-term disease progression in Pkd1RC/RC/Atm+/- mice. In vitro, human ADPKD cyst-derived cell lines were also resistant to cisplatin (WT9-12: 61.7 ± 4.6%; WT9-7: 64.8 ± 2.7% cell viability) compared to HK-2 (25.1 ± 4.2%), and 3D cyst growth in MDCK cells was not altered. Finally, combined low-dose cisplatin with AZD0156 (an ATM inhibitor) non-selectively reduced γH2AX in both cystic and non-cystic tubular cells and exacerbated cystic kidney disease. In conclusion, these data suggest that CECs are resistant to DNA damage, and that the combination of cisplatin with ATM inhibitors is not an effective strategy for selectively eliminating kidney cysts in ADPKD.


Assuntos
Cistos , Rim Policístico Autossômico Dominante , Camundongos , Humanos , Animais , Rim Policístico Autossômico Dominante/tratamento farmacológico , Rim Policístico Autossômico Dominante/genética , Rim Policístico Autossômico Dominante/metabolismo , Cisplatino/uso terapêutico , Proliferação de Células , Cistos/tratamento farmacológico , Cistos/metabolismo , Células Epiteliais/metabolismo , Dano ao DNA , Rim/metabolismo
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