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1.
Anticancer Res ; 39(8): 4191-4197, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31366505

RESUMO

BACKGROUND/AIM: Biopsy hole for bone tumour biopsy may cause pathological fractures. This study aimed to identify the optimal shape of bone tumour biopsy hole using the rabbit femoral head compression test to avoid pathological fracture. MATERIALS AND METHODS: A compression test with no defect was performed to identify bone fracture location. Three shapes of biopsy holes (same size) were made artificially. Sixty rabbit femurs were randomly divided (n=15 each) into control (no defect), Shape 1 (round), Shape 2 (square), and Shape 3 (rectangular) groups. RESULTS: Twelve out of fifteen femurs were fractured on the femoral shaft; the femoral shaft was targeted for the compression test. Compressive maximum load and fracture energy were significantly higher for Shape 3 than for the other Shapes. CONCLUSION: A rectangular biopsy hole helps minimise reduction in bone strength. The defect width may be related to fragility of the affected bone.


Assuntos
Biópsia/efeitos adversos , Neoplasias Ósseas/fisiopatologia , Fraturas do Fêmur/fisiopatologia , Fêmur/fisiopatologia , Animais , Fenômenos Biomecânicos , Neoplasias Ósseas/complicações , Neoplasias Ósseas/diagnóstico , Modelos Animais de Doenças , Fraturas do Fêmur/diagnóstico , Humanos , Coelhos
2.
Zhonghua Xin Xue Guan Bing Za Zhi ; 47(8): 608-613, 2019 Aug 24.
Artigo em Chinês | MEDLINE | ID: mdl-31434431

RESUMO

Objective: To investigate the effects and mechanism of digoxin on atrium electrical remodeling and susceptibility of atrial fibrillation (AF) in aged rabbits. Methods: Twenty aged male New Zealand rabbits were divided into aged group and aged plus digoxin group (n=10 each). Electrical parameters including heart rate (HR), RR and QT interval, ST segment and P wave dispersion from normal Ⅱ electrocardiogram, and the maximum upstroke velocity (Max(dv/dt)), plateau potential (plateau P), action potential duration of 10%, 20% and 90% (APD(10), APD(20), APD(90)) from recording of monophasic action potential (MAP), as well as atrial effective refractory period (AERP(200)) and dispersion (dERP(200)) with 200 ms of basic cycle length (BCL), and frequency self adaptation of AERP with 300 ms and 150 ms of BCLs (fERP) were recorded and compared between the 2 groups. BCLs and inducibility of AF post programmed electrical stimulation and Burst-pacing in left atrium tissue of rabbits in vivo were also analyzed. The L-type calcium current (I(Ca-L)) in 2 groups were recorded via whole-cell patch clamp technique, and the fluorescence intensity of intracellular free Ca(2+) was detected with Flup-3/AM loading by the laser scanning confocal microscope in enzymatically dissociated single rabbit atrial myocytes. Results: Compared with aged group, the heart rate was faster, RR and QT interval were obvious shorter, ST segment was raised and P wave dispersion was significantly increased in aged plus digoxin group (all P<0.05). Moreover, compared with aged group, the Max(dv/dt) and plateau P were obviously increased, APD(10) and APD(20) were significantly prolongated, and APD(9)0 was significantly shorter in aged plus digoxin group (all P<0.01). Otherwise, the fERP was markedly increased (0.81±0.15 vs. 0.67±0.05), and the induced rate of AF was obviously higher in aged plus digoxin group than in aged group (6/8 vs. 4/9) (all P<0.01). With voltage clamp model, digoxin significantly increased I(Ca-L) of atrial myocytes of aged rabbits, When command potential was 10 mV, the current densities of I(Ca-L) were significantly higher in digoxin group than that in aged group ((15.45±2.38) pA/pF vs. (7.03±1.69) pA/pF, P<0.01). Otherwise, the I-V curve of I(Ca-L) was downward shifted of all I-V curves in digoxin perfused aged atrial cells of rabbits. Moreover, the fluorescence intensities of intracellular free Ca(2+) was significantly higher in aged plus digoxin group than in aged group ((1 748±173) µmol/L vs. (478.13±87.63) µmol/L, P<0.01). Conclusion: Digoxin could aggravate the atrial electrical remodeling in atrium of aged rabbits, facilitate susceptibility of atrial fibrillation in aged rabbit, increased current density of I(Ca-L) and concentration of intracellular free Ca(2+), followed Ca(2+) overload and oscillations might be part of the underlying mechanisms.


Assuntos
Fibrilação Atrial , Remodelamento Atrial , Potenciais de Ação , Animais , Digoxina , Átrios do Coração , Masculino , Técnicas de Patch-Clamp , Coelhos
3.
Am J Orthod Dentofacial Orthop ; 156(1): 75-86, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31256844

RESUMO

INTRODUCTION: Maintaining tooth anchorage during orthodontic treatment has challenged orthodontists and threatening the success of some orthodontic therapy. The objective of this study was to evaluate the effect of local administration of simvastatin on orthodontic tooth movement. METHODS: Nickel-titanium coil springs were used to induce orthodontic tooth movement in 10 white New Zealand rabbits for 21 days. A split-mouth design was implemented where one mandibular quadrant received local administration of simvastatin and the corresponding mandibular quadrant received control vehicle solution on a weekly basis. Magnitudes of tooth movement were measured on 3-dimensional models of the experimental teeth. Animals were killed at the end of the experimental period to allow histomorphometric analysis of alveolar bone modeling. RESULTS: The total magnitude of tooth movement in the quadrant receiving simvastatin was significantly less than that in the quadrant receiving control vehicle solution. Local administration of simvastatin resulted in a significant percentage of inhibition of tooth movement of 39.8 ± 22.6%. Histomorphometric analysis revealed a significant reduction in the numbers of osteoclasts and areas of active bone-resorptive lacunae hindering bone resorption processes in the quadrant receiving simvastatin. CONCLUSIONS: Local administration of simvastatin can reduce the rate and magnitude of orthodontic tooth movement. Moreover, local administration of simvastatin diminishes bone resorption processes associated with orthodontic tooth movement reducing the number of osteoclasts and the subsequent area of active bone resorption.


Assuntos
Reabsorção Óssea/patologia , Osteoclastos/efeitos dos fármacos , Sinvastatina/administração & dosagem , Sinvastatina/antagonistas & inibidores , Técnicas de Movimentação Dentária/métodos , Perda do Osso Alveolar/diagnóstico por imagem , Perda do Osso Alveolar/patologia , Animais , Remodelação Óssea/efeitos dos fármacos , Reabsorção Óssea/diagnóstico por imagem , Masculino , Mandíbula/irrigação sanguínea , Mandíbula/efeitos dos fármacos , Mandíbula/patologia , Modelos Animais , Níquel/química , Aparelhos Ortodônticos , Fios Ortodônticos , Osteoclastos/patologia , Coelhos , Titânio/química , Técnicas de Movimentação Dentária/instrumentação
4.
Zhonghua Yi Xue Za Zhi ; 99(24): 1898-1903, 2019 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-31269587

RESUMO

Objective: To investigate the effect of different drugs on tracheal stenosis caused by transforming growth factor-ß/rapamycin target protein (TGF-ß/mTOR) signaling pathway. Methods: Thirty rabbits were randomly divided into normal control group, normal saline group, penicillin group, budesonide group and erythromycin group. The normal control group was not treated,and tracheal stenosis models were established in the other groups. From the 1st to 10th day after modeling, each group was respectively administered with normal saline (0.75 ml/kg, 2 times/d), intramuscular injection of penicillin (40 000 U/kg, 2 times/d), gastric administration of erythromycin (12.5 mg/kg, 2 times/d), inhalation of budesonide (0.05 mg/kg, 2 times/d). Rabbits were sacrificed on the 11th day after surgery, and tracheal specimens were collected to measure the degree of tracheal stenosis. Relative mRNA expression level of interleukin-6 (IL-6), transforming growth factor-ß (TGF-ß), Type Ⅰ collagen (COL-1), Type Ⅲ collagen (COL-3), and Sirtuin 1 (SIRT-1) were detected by Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR); protein expression of mTOR, phosphorylated protein kinase B (p-AKT), vascular endothelial growth factor (VEGF),SIRT-1 were detected by immunohistochemical analysis; protein expression of nuclear factor κB (NF-κB),phosphorylated nuclear factor κB (p-NF-κB),protein kinase B (AKT),p-AKT,mTOR were detected by Western blotting. Results: The degree of stenosis of normal control group was (14.02±2.86)%, saline group was (64.14±3.21)%, penicillin group was (49.11±2.96)%, budesonide group was (39.52±2.09)%, erythromycin group was (32.60±4.27)%. The differences between any two groups were statistically significant (all P<0.05). Except between erythromycin group and normal control group, the differences in relative expression of IL-6 mRNA between any two groups (1.00±0.00, 9.02±1.50, 4.25±0.87, 2.53±0.17, 1.31±0.56) was statistically significant (all P<0.05), and the differences in relative expression of TGF-ß mRNA among all groups (1.00±0.00, 6.92±0.84, 3.83±0.44, 2.13±0.25, 1.40±0.15) were statistically significant (all P<0.05). The relative expression of SIRT-1 mRNA among all the groups (1.000±0.000, 0.209±0.042, 0.375±0.034, 0.555±0.028, 0.667±0.032) was statistically significant different (all P<0.05); except between erythromycin group and budesonide group,the protein levels of SIRT-1 among all other groups (16.93±2.28, 4.77±1.45, 7.70±0.61, 10.76±1.04, 11.03±1.10) were statistically significant different (all P<0.05). The protein levels of mTOR (9.28±4.56, 58.18±8.12, 44.75±5.56, 32.82±5.99, 24.73±3.56) and p-AKT (16.57±4.86, 61.79±6.66, 42.98±5.99, 32.79±5.34, 24.00±4.40) determined through immunohistochemistry of all groups were statistically significant different (all P<0.05). The protein levels of NF-κB, p-NF-κB, AKT, p-AKT and mTOR determined through Western blotting had the same trend as that of determined through immunohistochemistry. The protein expression of NF-κB,AKT and mTOR in saline group were significantly higher than other groups; those protein expression of erythromycin group was lower than budesonide group and penicillin group. Except between the erythromycin group and the normal control group, the protein expression of mTOR in other groups was statistically significant different (all P<0.05). Conclusion: Penicillin,erythromycin and budesonide can alleviate inflammation by increasing SIRT-1, alleviate tracheal scar hyperplasia induced by TGF-beta/mTOR pathway, and reduce the degree of tracheal stenosis in rabbits.


Assuntos
Constrição Patológica , Animais , Broncopatias , Preparações Farmacêuticas , Coelhos , Transdução de Sinais , Serina-Treonina Quinases TOR , Fator de Crescimento Transformador beta , Fator A de Crescimento do Endotélio Vascular
5.
J Pediatr Ophthalmol Strabismus ; 56(4): 254-260, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31322717

RESUMO

PURPOSE: To determine whether suturing to the residual muscle stump induces postoperative muscle migration after superior rectus muscle (SRM) resection in rabbit eyes. METHODS: Twenty-four eyes in 12 rabbits were randomly divided into two groups. Each group underwent a 3-mm SRM resection. The muscle stump was not removed in one randomly assigned eye in each rabbit and the resected muscle was sutured to the residual muscle stump (residual muscle stump group). In the other eye, the muscle stump was completely removed and the resected muscle was sutured to the original insertion site (control group). At postoperative weeks 1, 2, and 4, the distances between the most anterior aspect of the reattached muscle and the corneal limbus were measured. Histopathologic differences at the new attachment sites were assessed between the two groups at postoperative week 4. RESULTS: The mean distance between the corneal limbus and SRM was 2.97 ± 1.00 mm in the residual muscle stump group and 1.99 ± 0.40 mm in the control group at postoperative week 2 (P = .026) and 3.14 ± 0.78 mm in the residual muscle stump group and 1.81 ± 0.44 mm in the control group at postoperative week 4 (P = .002). Connective tissue, fibrosis, and myofibroblast proliferation in the front of the SRM were identified based on histopathologic findings. CONCLUSIONS: Postoperative loosening associated with a stretched scar frequently occurred in cases with suturing to the residual muscle during rectus muscle resection and could result from fibrosis and connective tissue proliferation in front of the rectus muscle. [J Pediatr Ophthalmol Strabismus. 2019;56(4):254-260.].


Assuntos
Cicatriz/etiologia , Músculos Oculomotores/cirurgia , Complicações Pós-Operatórias , Estrabismo/cirurgia , Técnicas de Sutura/efeitos adversos , Suturas/efeitos adversos , Animais , Cicatriz/patologia , Modelos Animais de Doenças , Músculos Oculomotores/diagnóstico por imagem , Coelhos
6.
Biomed Khim ; 65(3): 227-230, 2019 Apr.
Artigo em Russo | MEDLINE | ID: mdl-31258146

RESUMO

The experimental study in vivo was aimed at evaluation of hypolipidemic action of the original natural microbial enzyme preparation of cholesterol oxidase (CHO). In preliminary chronic experiments in rats, rabbits, dogs, low toxicity, good tolerability, and anti-atherosclerotic activity of the CHO preparation were established. To assess the effect of CHO under conditions of moderate, nutritional, atherogenic dyslipoproteinemia, experiments were carried out in rats, guinea pigs, and rabbits. It was shown that administration of CHO had the pronounced lipid-lowering effect in models of atherogenic dyslipoproteinemia induced in these animals.


Assuntos
Aterosclerose/tratamento farmacológico , Colesterol Oxidase/farmacologia , Hipolipemiantes/farmacologia , Lipídeos/sangue , Animais , Cães , Cobaias , Coelhos , Ratos
7.
Acta Neurol Taiwan ; 28(1): 1-11, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31321759

RESUMO

OBJECTIVE: Vagal nerves and their thermoreceptors could regulate temperature of brain. Cerebrospinal fluid (CSF) is increased in the early phases of subarachnoid hemorrhage (SAH). We hypothesised that choroid plexuses probably innervated by vagal nerves may play a role on the regulation of brain temperature and studied this subject. METHODS: This study was conducted on 32 rabbits, divided into four groups, with five rabbits in the control group (group I), five rabbits in the sham group (Group II), and 22 rabbits in the SAH group. In the SAH group, 7 of the animals were decapitated after 7 days of cisternal blood injections (Group III), and the other 15 animals were decapitated after 21 days of injections (Group IV). Brain temperature via laser thermometer 5 times a day during the experiment was measured. Normal and degenerated neuron density of nodose ganglia, water vesicles numbers of choroid plexuses were stereologicallyanalyzed. Statistical analysis was performed. RESULTS: At histopathologic analysis of present study, thermo regulator like structure was noted and the mean number of this structure was estimated.The mean number of water-filled vesicles, thermo regulator like structure, in SAH-induced animals,brain temperature and degenerated neuron density of nodose ganglia was statistically different between the early decapitated group (group III) and the late decapitated group (group IV) (P less then 0.05). CONCLUSIONS: We introduce a thermo regulator like structure, describe a new syndrome. In addition, it was noted thatwater-filled vesicles of CP are increased, brain temperature in nearly normal in the early phase of SAH due to likely irritation of vagal nerves. However in the late phase, mean number of water-filled vesicles numbers decreased in accordance with increased brain temperature with degenerative changes of the nodose ganglion.


Assuntos
Hemorragia Subaracnóidea , Animais , Encéfalo , Corioide , Febre , Degeneração Neural , Coelhos
8.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(5): 477-484, 2019 May 28.
Artigo em Chinês | MEDLINE | ID: mdl-31303609

RESUMO

OBJECTIVE: To investigate the value of the total liver CT perfusion imaging in the evaluation of rabbit VX2 liver tumors treated with TACE and apatinib.
 Methods: Thirty-six rabbit VX2 liver cancer models were established and randomly divided into 4 groups. Group A: simple TACE group; Group B: simple oral administration of apatinib mesylate; Group C: TACE + oral apatinib mesylate; Group D: control group, administration of saline. CT perfusion imaging (CTPI) was performed before treatment and on the 7 and 14 days after the treatment to acquire perfusion parameters including blood flow (BF), blood volume (BV), MTT (mean transit time), surface permeability (PS), and hepatic artery fraction (HAF). One tumor rabbit was sacrificed in each group after the first perfusion scan, and the remaining tumor rabbits were sacrificed after the last perfusion scan on the 14th day of the treatment. The borders of the tumors were stained immunohistochemically, and microvascular density (MVD) was measured by anti-CD34. The differences of perfusion parameters were compared to evaluate the liver hemodynamic changes, and statistical repeated measurement variance analysist correlation analysis were performed.
 Results: There were no significant differences in CTPI parameters of BF, BV, MTT, HAF and PS between the 4 groups before treatment (P>0.05). After the treatment, HB, HAF and PS were decreased significantly in Group A, B, and C and slightly increased in the Group D. The value of MVD after 14 d treatment was 80.1±16.4 in Group A, 50.2±11.2 in Group B, 27.4±9.7 in Group C, 68.7±12.7 in Group D, respectively. The value of MVD in the Group C were significantly lower than that in Group A, B, and D. It showed positive correlation between BF, HAF, PS and MVD in Group B, C, and D, and there was no significant correlation between BV, MTT and MVD. It showed no significant correlation between MVD and each CTPI parameter in Group A.
 Conclusion: Total liver CT perfusion can quantitatively evaluate the blood perfusion information of rabbit liver VX2 tumor after TACE. TACE combined with oral apatinib can effectively inhibit tumor growth and improve the therapeutic effect of VX2 tumor.


Assuntos
Carcinoma Hepatocelular , Quimioembolização Terapêutica , Neoplasias Hepáticas , Animais , Neovascularização Patológica , Imagem de Perfusão , Piridinas , Coelhos , Tomografia Computadorizada por Raios X
9.
Bone Joint J ; 101-B(7): 824-831, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31256666

RESUMO

AIM: Mesenchymal stem cells (MSCs) have several properties that may support their use as an early treatment option for osteoarthritis (OA). This study investigated the role of multiple injections of allogeneic bone marrow-derived stem cells (BMSCs) to alleviate the progression of osteoarthritic changes in the various structures of the mature rabbit knee in an anterior cruciate ligament (ACL)-deficient OA model. MATERIALS AND METHODS: Two months after bilateral section of the ACL of Japanese white rabbits aged nine months or more, either phosphate buffered saline (PBS) or 1 x 106 MSCs were injected into the knee joint in single or three consecutive doses. After two months, the articular cartilage and meniscus were assessed macroscopically, histologically, and immunohistochemically using collagen I and II. RESULTS: Within the PBS injection (control group), typical progressive degenerative changes were revealed in the various knee structures. In the single MSC injection (single group), osteoarthritic changes were attenuated, but still appeared, especially in the medial compartments involving fibrillation of the articular cartilage, osteophyte formation in the medial plateau, and longitudinal tear of the meniscus. In the multiple-injections group, the smoothness and texture of the articular cartilage and meniscus were improved. Histologically, absence or reduction in matrix staining and cellularity were noticeable in the control and single-injection groups, respectively, in contrast to the multiple-injections group, which showed good intensity of matrix staining and chondrocyte distribution in the various cartilage zones. Osteoarthritis Research Society International (OARSI) scoring showed significantly better results in the multiple-injections group than in the other groups. Immunohistochemically, collagen I existed superficially in the medial femoral condyle in the single group, while collagen II was more evident in the multiple-injections group than the single-injection group. CONCLUSION: A single injection of MSCs was not enough to restore the condition of osteoarthritic joints. This is in contrast to multiple injections of MSCs, which had the ability to replace lost cells, as well as reducing inflammation. Cite this article: Bone Joint J 2019;101-B:824-831.


Assuntos
Transplante de Células-Tronco Mesenquimais/métodos , Osteoartrite do Joelho/terapia , Animais , Ligamento Cruzado Anterior/cirurgia , Injeções Intra-Articulares , Osteoartrite do Joelho/etiologia , Osteoartrite do Joelho/patologia , Coelhos , Transplante Homólogo , Resultado do Tratamento
10.
Vet Comp Orthop Traumatol ; 32(4): 297-304, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31319431

RESUMO

OBJECTIVE: The aim of this study was to evaluate the biomechanical properties of three different miniature locking plate systems used to fixate radial and ulnar fractures in toy breed dogs. Implant size, shape, material and locking systems differ, and their influence on the fracture healing process is unknown. In the present study, we aimed to investigate this matter in vivo using rabbit radial and ulnar fracture models. STUDY DESIGN: Eighteen rabbits were randomly divided into three groups, and the left radius and ulna were osteotomized to create fracture models. The osteotomies were then fixated using either the TITAN LOCK 1.5, Fixin micro or LCP 1.5 system. Radiographs were obtained 2, 3 and 4 weeks after surgery. Four weeks after surgery, the radiuses were collected and used for biomechanical testing or histological examinations. RESULTS: During the 4 weeks of observation, no adverse effects due to the implants occurred. The radiographic scores in each group did not differ significantly at any time point. The maximum load in the LCP group was significantly higher than that in the TITAN and Fixin groups. There was no significant difference in bending stiffness or work to failure among the groups. Initial fracture healing via woven bone was evident at histological evaluation. CONCLUSIONS: All three miniature locking plate systems provided adequate fracture stabilization 4 weeks after surgery, despite their differences, in rabbit models.


Assuntos
Placas Ósseas/veterinária , Coelhos , Fraturas do Rádio/veterinária , Fraturas da Ulna/veterinária , Animais , Fenômenos Biomecânicos , Placas Ósseas/efeitos adversos , Modelos Animais de Doenças , Cães , Consolidação da Fratura , Masculino , Miniaturização , Período Pós-Operatório , Radiografia/veterinária , Fraturas do Rádio/tratamento farmacológico , Fraturas do Rádio/patologia , Fraturas do Rádio/cirurgia , Distribuição Aleatória , Fraturas da Ulna/diagnóstico por imagem , Fraturas da Ulna/patologia , Fraturas da Ulna/cirurgia
11.
Pol J Microbiol ; 68(2): 173-183, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31257790

RESUMO

In this research, Salmonella species were isolated from the animal, insect and human enteric sources in Faisalabad, Punjab, Pakistan. These species were characterized by different microbiological and molecular techniques including polymerase chain reaction (PCR) by amplification of the 16S rRNA gene. Furthermore, sequencing of the amplicons confirmed all ten isolates as Salmonella strains. The antigenic cross-reactivity was found maximum between the HB1 (strain isolated from honeybee) antiserum and its antigen with an antibody titer of 1:128, while the HB1 antiserum showed a cross-reactive titer range of 1:8 to 1:64. On the basis of the highest geometric mean titer (GMT) shown by the antiserum of the HB1 antigen, it was selected as the best candidate for a cross-reactive live Salmonella oral antigen. Moreover, the HB1 antigen was used a live oral antigen (1 × 1010 CFU/ml) in a safety test in rabbits and proved to be avirulent. During the animal trial, three different oral doses of the HB1 live oral antigen were evaluated in four different rabbits' groups (R1, R2, R3, and R4). The dose number 2 of 0.5 ml (two drops orally and repeated after one week) gave the best GMT measured by indirect hemagglutination (IHA) as compared to the other two doses, while R4 group was kept as control. Results of the challenge protection test also validated the efficacy of the double dose of the HB1 live vaccine, which gave the highest survival percentage. Results of this study lay the foundation for a potential cross-reactive live oral Salmonella vaccine that has proved to be immunogenic in rabbits.In this research, Salmonella species were isolated from the animal, insect and human enteric sources in Faisalabad, Punjab, Pakistan. These species were characterized by different microbiological and molecular techniques including polymerase chain reaction (PCR) by amplification of the 16S rRNA gene. Furthermore, sequencing of the amplicons confirmed all ten isolates as Salmonella strains. The antigenic cross-reactivity was found maximum between the HB1 (strain isolated from honeybee) antiserum and its antigen with an antibody titer of 1:128, while the HB1 antiserum showed a cross-reactive titer range of 1:8 to 1:64. On the basis of the highest geometric mean titer (GMT) shown by the antiserum of the HB1 antigen, it was selected as the best candidate for a cross-reactive live Salmonella oral antigen. Moreover, the HB1 antigen was used a live oral antigen (1 × 1010 CFU/ml) in a safety test in rabbits and proved to be avirulent. During the animal trial, three different oral doses of the HB1 live oral antigen were evaluated in four different rabbits' groups (R1, R2, R3, and R4). The dose number 2 of 0.5 ml (two drops orally and repeated after one week) gave the best GMT measured by indirect hemagglutination (IHA) as compared to the other two doses, while R4 group was kept as control. Results of the challenge protection test also validated the efficacy of the double dose of the HB1 live vaccine, which gave the highest survival percentage. Results of this study lay the foundation for a potential cross-reactive live oral Salmonella vaccine that has proved to be immunogenic in rabbits.


Assuntos
Antígenos de Bactérias/imunologia , Abelhas/microbiologia , Salmonelose Animal/prevenção & controle , Salmonella typhimurium/imunologia , Salmonella typhimurium/isolamento & purificação , Vacinas Tíficas-Paratíficas/imunologia , Animais , Antígenos de Bactérias/administração & dosagem , DNA Bacteriano/genética , Fezes/microbiologia , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , Coelhos , Salmonelose Animal/microbiologia , Salmonella typhimurium/classificação
12.
J Photochem Photobiol B ; 197: 111545, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31326847

RESUMO

Proper waste utilization in order to promote value added product is a promising scientific practice in recent era. Inspiring from the recurring trend, we propose a single step oxidative pyrolysis derived fluorescent carbon dots (C-dots) from Allium sativum peel, which is a natural, nontoxic, and waste raw material. Because of its excellent optical properties, and photostability this C-dots have been used in versatile area of applications. Due to its immediate water dispersing character, C-dots reinforced Poly(acrylic acid) (PAA) films revealed improvement in uniaxial stretching behavior and can be used as transparent sunlight conversion film. The nanocomposite film has been tested against rigorous simulated sunlight which proved almost identical sunlight conversion behavior with no photo-bleachable character which is definitely added an extra quality of transparent polymer films. Moreover, the C-dots dispersion has been used as in vitro biomarker for living cells owing to its ease in solubility, biocompatibility, non-cytotoxicity and bright fluorescence even in subcutaneous environment. For this case, adipose derived mesenchymal stem cells (ADMSCs) have been chosen and injected to rabbit ear skin to perform two-photon imaging experiment. The present work opens a new avenue towards the large-scale synthesis of bio-waste based fluorescent C-dots, paving the way for their versatile applications.


Assuntos
Allium/química , Nitrogênio/química , Fotodegradação/efeitos da radiação , Pontos Quânticos/química , Enxofre/química , Luz Solar , Resinas Acrílicas/química , Tecido Adiposo/citologia , Allium/metabolismo , Animais , Materiais Biocompatíveis/química , Carbono/química , Sobrevivência Celular/efeitos dos fármacos , Frutas/química , Frutas/metabolismo , Química Verde , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Microscopia de Fluorescência , Pontos Quânticos/toxicidade , Coelhos , Pele/efeitos dos fármacos , Pele/patologia , Solubilidade
13.
BMC Ophthalmol ; 19(1): 130, 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31208396

RESUMO

BACKGROUND: Nerve growth factor (NGF), produced by Müller cells, and internal limiting membrane (ILM) have fundamental roles in the development of full-thickness macular hole (FTMH). However, the potential crosstalk between NGF and ILM in FTMH is unclear. This study aimed to explore the mechanism and effects of NGF on the proliferation of Müller cells co-cultured with ILM. METHODS: Primary Müller cells and ILM from New Zealand rabbits were extracted and authenticated with specific staining. Müller cells co-cultured with or without ILM were exposed to NGF and then analysed. Müller cell viability was estimated using cell counting kit-8. Cell cycle analysis was performed by flow cytometry. The levels of cell cycle-related gene were detected using qRT-PCR. The TrK-A/Akt signal axis and downstream signaling cascades such as p21, CyclinE, CDK2, CyclinD1, and CDK4 were investigated by western blotting. RESULTS: ILM treatment alone induced the proliferation of Müller cells following the promotion of phosphorylated Akt, while growth of Müller cells was enhanced by activation of the Trk-A/Akt pathway under the stimulation of NGF or NGF + ILM. Additionally, the ratio of S-phase cells was increased, while G2-phase cells decreased upon the treatment with either ILM or NGF alone, or with NGF + ILM co-treatment. Cell cycle-related genes such as CyclinD1, CyclinE, CDK2, and CDK4 were all upregulated, but p21 expression was downregulated in the presence of NGF, ILM, or NGF + ILM. There was an additive effect on cell proliferation and cell cycle in the group of Müller cells exposed to NGF co-cultured with ILM compared with either NGF or ILM treatment alone. However, both K252ɑ (inhibitors of Trk-A) and LY294002 (inhibitor for Akt) counteracted the effect of NGF or NGF + ILM on the protein levels of Trk-A, Akt, CyclinD1, CyclinE, CDK2, and p21. CONCLUSIONS: Müller cells co-cultured with ILM or NGF promoted cell proliferation by regulating cell cycle-correlated proteins via the PI3K/Akt pathway. ILM + NGF further amplified the PI3K/Akt signaling pathway by binding to Trk-A, leading to more cell growth. This study provides new insight into the potential mechanism of NGF-mediated proliferation of Müller cells co-cultured with or without ILM, which may have considerable impact on therapies for FTMH.


Assuntos
Membrana Basal , Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Ependimogliais/efeitos dos fármacos , Fator de Crescimento Neural/farmacologia , Fosfatidilinositol 3-Quinases/fisiologia , Proteínas Proto-Oncogênicas c-akt/fisiologia , Animais , Membrana Basal/efeitos dos fármacos , Membrana Basal/fisiologia , Técnicas de Cocultura , Células Ependimogliais/citologia , Coelhos
14.
J Biomed Nanotechnol ; 15(3): 602-611, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31165704

RESUMO

Interpenetrating network structures from Graphene foam (GF) and 58S bioactive glass (BG) are synthesized to combine the highly mechanical stability and conductivity from graphene with the superb bioactivity and biocompatibility from 58S BG. GF/58S BG scaffolds were prepared via multiple steps including chemical vapor deposition (CVD), spin-coating, and freeze drying methods. Simulated body fluid test confirms the highly bioactivity of the as-synthesized GF/58S BG scaffold after incorporating of sol-gel derived 58S BG. The GF/58S BG scaffold also remains good electrical conductivity of graphene after combination of 58S BG. Biocompatibility of both GF and GF/58S BG scaffold against the rabbit mesenchymal stem cells (rMSCs) is studied. Both GF and GF/58S BG scaffold facilitate the adhesion and extension of rMSCs, while the GF/58S BG scaffold shows a higher proliferation. Electrical stimulation was further applied on the both GF and GF/58S BG scaffold. Both scaffolds promote the osteogenic differentiation of rMSCs, while GF is more sensitive to the alternating electrical current. In vivo results based on the critical-sized radius defect rabbit model confirmed that the resulting GF/58S BG scaffold considerably promoted the formation of new bone. Our studies suggest that the assynthesized GF/58S BG scaffolds are the promising candidates for bone tissue engineering and electrically stimulated regeneration considering unique bioactive, biocompatible, conductive and stable properties of the resulting nanoscaffolds.


Assuntos
Estimulação Elétrica , Grafite , Células-Tronco Mesenquimais , Animais , Regeneração Óssea , Diferenciação Celular , Vidro , Osteogênese , Coelhos , Tecidos Suporte
15.
Khirurgiia (Mosk) ; (5): 52-56, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31169819

RESUMO

AIM: Experimental analysis of the strength properties of integration of muscle tissue, tendons and ligaments, bone tissue into titanium highly porous materials prepared by using of additive technologies. MATERIAL AND METHODS: The study included 9 mature rabbits of the Chinchilla breed. Both posterior paws and latissimus dorsi muscles (36 specimens) were used. Titanium models (Ti-6-Al-4-V, 'Rematitan', Germany) were made by additive technologies with preliminary prototyping, the prototypes had a highly porous mesh structure. RESULTS: It was developed experimental model of functional bone defect involving points of attachment of the muscle and ligaments. Strength of attachment of muscles to highly porous implants was 145 H vs. 31 H for standard models. Tensile strength for fixation to bone tissue was 84 H vs. 152 H in the main group. CONCLUSION: It was found integration of soft tissues and bones into highly porous titanium implants prepared with additive technologies. Fixation strength significantly exceeds that for standard models.


Assuntos
Osso e Ossos/cirurgia , Sistema Musculoesquelético/fisiopatologia , Osseointegração/fisiologia , Próteses e Implantes , Cicatrização/fisiologia , Animais , Materiais Biocompatíveis , Osso e Ossos/lesões , Osso e Ossos/fisiopatologia , Ligamentos/fisiopatologia , Ligamentos/cirurgia , Modelos Animais , Músculo Esquelético/fisiopatologia , Músculo Esquelético/cirurgia , Porosidade , Desenho de Prótese , Implantação de Prótese , Coelhos , Recuperação de Função Fisiológica , Reoperação , Telas Cirúrgicas , Tendões/fisiopatologia , Tendões/cirurgia , Titânio
16.
Folia Histochem Cytobiol ; 57(2): 94-100, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31237344

RESUMO

INTRODUCTION: A reduced number of interstitial Cajal-like cells (ICLCs) in the gallbladder have been proposed to play a role in the pathogenesis of cholelithiasis. Therefore, this prospective study was conducted to investigate the relationship between gallbladder contractility and the number of gallbladder ICLCs in patients with cholelithiasis. MATERIAL AND METHODS: Patients admitted to the Department of Hepatobiliary Surgery for cholecystectomy were divided into the cholelithiasis (n = 18) and non-cholelithiasis (n = 8) groups based on their clinical data. Patients' clinical data were collected on admission, and B-mode ultrasonography was performed to assess their gallbladder contractility. The resected gallbladder specimens were fixed, paraffin sections mounted on slides, and the immunofluorescence staining with the anti-human CD-117 and anti-human tryptase antibodies was performed to identify ICLSs and mast cells, respectively. The number of ICLCs was counted in 10 high-power fields (HPFs) randomly. RESULTS: Independent sample t-tests revealed differences between the cholelithiasis and non-cholelithiasis groups in the number of ICLCs (mean ± standard deviation: 88.61 ± 28.22 vs. 115.89 ± 27.87 per HPFs, P = 0.032) and gallbladder contractility (43.94% ± 18.50% vs. 61.00% ± 20.50%, P = 0.046). Pearson and Spearman cor-relation analyses revealed no significant correlation between the number of ICLCs and gallbladder contractility. CONCLUSION: The results suggest that the number of gallbladder ICLCs in the wall of the gallbladder of patients with or without cholelithiasis is not a decisive factor affecting gallbladder contractility.


Assuntos
Colelitíase/fisiopatologia , Esvaziamento da Vesícula Biliar/fisiologia , Vesícula Biliar/citologia , Vesícula Biliar/fisiologia , Telócitos/citologia , Adulto , Idoso , Animais , Anticorpos/imunologia , Contagem de Células , Colelitíase/patologia , Feminino , Vesícula Biliar/patologia , Cabras , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , Estudos Prospectivos , Proteínas Proto-Oncogênicas c-kit/imunologia , Coelhos , Telócitos/patologia , Triptases/imunologia
17.
Cell Prolif ; 52(4): e12587, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31206838

RESUMO

OBJECTIVES: Cellular aggregates are readily applicable in cell-based therapy. The effects of agitation and inoculation density on the aggregation of cells in spinner flask and the molecular mechanism of aggregation were investigated. MATERIALS AND METHODS: The aggregation kinetics of cells in spinner flask was evaluated with bovine articular chondrocytes (bACs), rabbit bone marrow-derived mesenchymal stem cells (rMSCs) and their mixture. The morphology of cellular aggregates was studied with scanning electron microscopy and gene expression of cell adhesion-related molecules was analysed. RESULTS: It was shown that suspension culture in spinner flask induced the aggregation of bACs and rMSCs. Both cells exhibited increased aggregation rate and aggregate size with decreasing agitation rate and increasing cell inoculation density. Additionally, aggregate size increased with extended culture time. By analysing gene expression of integrin ß1 and cadherin, it was indicated that these molecules were potentially involved in the aggregation process of bACs and rMSCs, respectively. Aggregates composed of both bACs and rMSCs were also prepared, showing rMSCs in the core and bACs in the periphery. CONCLUSIONS: Cellular aggregates were prepared in dynamic suspension culture using spinner flask, the key parameters to the aggregation process were identified, and the molecular mechanism of aggregation was revealed. This would lay a solid foundation for the large-scale production of cellular aggregates for cell-based therapy, such as cartilage regeneration.


Assuntos
Condrócitos/citologia , Células-Tronco Mesenquimais/citologia , Animais , Medula Óssea/metabolismo , Medula Óssea/fisiologia , Bovinos , Adesão Celular/fisiologia , Moléculas de Adesão Celular/metabolismo , Contagem de Células/métodos , Técnicas de Cultura de Células/métodos , Terapia Baseada em Transplante de Células e Tecidos/métodos , Células Cultivadas , Condrócitos/metabolismo , Expressão Gênica/fisiologia , Células-Tronco Mesenquimais/metabolismo , Coelhos
18.
Am J Vet Res ; 80(7): 657-662, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31246125

RESUMO

OBJECTIVE: To use duplex Doppler ultrasonography to compare gastrointestinal activity in healthy sedated versus nonsedated rabbits and to evaluate agreement between B-mode and pulsed-wave Doppler (PWD) ultrasonographic measurements. ANIMALS: 10 healthy client-owned rabbits brought for routine physical examination and 11 brought for routine ovariohysterectomy or castration. PROCEDURES: Duplex Doppler ultrasonography of the gastrointestinal tract was performed once for the 10 rabbits that underwent physical examination and twice (before and after presurgical sedation) for the 11 rabbits that underwent routine ovariohysterectomy or castration. Mean number of peristaltic contractions during a 30-second period was determined for the stomach, duodenum, jejunum, cecum, and colon from B-mode and PWD ultrasonographic images that had been video recorded. Findings for the duodenum and jejunum were compared between B-mode and PWD ultrasonography and between sedated and nonsedated rabbits. RESULTS: Duodenal and jejunal segments had measurable peristaltic waves; however, the stomach, cecum, and colon had no consistent measurable activity. B-mode and PWD ultrasonographic measurements for the duodenum and jejunum had high agreement. No significant difference was identified between nonsedated and sedated rabbits in mean number of peristaltic contractions of the duodenum or jejunum. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that both B-mode and PWD ultrasonography of the duodenum and jejunum may be suitable for noninvasive evaluation of small intestinal motility in rabbits and that the sedation protocol used in this study had no impact on measured peristaltic values.


Assuntos
Trato Gastrointestinal/fisiologia , Peristaltismo , Coelhos/fisiologia , Ultrassonografia Doppler Dupla/veterinária , Ultrassonografia Doppler de Pulso/veterinária , Animais , Sedação Consciente/veterinária , Sedação Profunda/veterinária , Feminino , Masculino
19.
J Pediatr Orthop ; 39(6): 289-294, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31169748

RESUMO

BACKGROUND: There are studies on the use of oblique plate over epiphyses for rotational deformities but the effects of this method on bone are not yet known. The purpose of this study was to determine the effect and rebound effect of rotational-guided growth on the geometries of the tibial plateaus and menisci in a rabbit model of tibial axial rotation. METHODS: Thirty male rabbits were 6 weeks old when medial and lateral plates were applied to the proximal tibias of the left side. After 4 weeks, 15 rabbits were euthanized (group 1), and the plates from the tibias of the remaining 15 rabbits (group 2) were removed. The rabbits in group 2 were euthanized 4 weeks later. RESULTS: In the rabbits of group 1, the most striking differences were a decrease in the lateral tibial slope (from 28.3 to 10.8 degrees) and decrease in the ratio of the lateral plateau covered by the meniscus (from 71.9% to 61.3%). After removing the plates (group 2), the observed values of the rebound effects were 25.9 and 29.8 degrees for the lateral tibial slope, and 76.5% and 77.2% for the ratio of the lateral plateau covered by the meniscus. However, the meniscal geometries continued to change. CONCLUSIONS: The rotational-guided growth provided by using plates caused a change in the tibial plateau geometry, and the rebound effect, except in the meniscal geometry, was observed after removing the plates in the growing period of the rabbits. Rotational-guided growth for the restoration of tibial axial rotation deformities may be suggested after determining the clinical effects of the increasing meniscal variables. LEVEL OF EVIDENCE: Clinical relevance: the rotational-guided growth needs close follow-up because of possible changes in anatomy of the bone.


Assuntos
Placas Ósseas , Lâmina de Crescimento/patologia , Meniscos Tibiais/cirurgia , Tíbia/cirurgia , Animais , Modelos Animais de Doenças , Feminino , Humanos , Deformidades Congênitas das Extremidades Inferiores/cirurgia , Masculino , Meniscos Tibiais/patologia , Coelhos , Rotação , Tíbia/patologia
20.
Sheng Wu Gong Cheng Xue Bao ; 35(5): 901-909, 2019 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-31223008

RESUMO

To establish a novel colloidal gold immunochromatography assay (GICA) for rapid, sensitive and accurate detection of Haemophilus influenzae infection by using the outer membrane protein P6 as detection target. First, the linear antigen epitope located in the extracellular domain of the P6 protein (GenBank accession number: AGH02799) was predicted by bioinformatics analysis. The region (62-75 aa of the protein) with strong antigen specificity was chosen and synthesized. Two rabbits were then immunized by the polypeptides (14 aa) for production of polyclonal antibodies. Then, the recombinant P6 proteins were also obtained to produce polyclonal antibodies. Finally, based on the two antibodies, a novel colloidal GICA for detection of Haemophilus influenzae infection was established and the specificity, sensitivity, repeatability and stability of this method were evaluated. At the same time, the method was tested in clinical simulation, and the plate culture method was used to verify its accuracy. The test strip for Haemophilus influenzae infection was successfully prepared. The detection limit of the test strip was as low as 1×105 CFU/mL and the whole process can be completed within 15 minutes. The strip specifically recognized Haemophilus influenzae and did not react with nine of other common respiratory pathogens such as Streptococcus pneumoniae, Moraxella catarrhalis, Mycoplasma pneumonia, and Legionella pneumophila. And the strips could be stored at 25 °C for at least 6 months without losing sensitivity or specificity. The coincidence rate between the results of 200 clinical samples and the plate culture method was 90.5%. Haemophilus influenzae protein P6, which possessed a high degree of surface antigen accessibility and antigencity, could be used as a marker for Haemophilus influenzae detection. The immunochromatographic colloidal gold test strip which bears the features of rapidity, convenience and sensitivity provides a unique tool for the on-site surveillance and diagnosis of Haemophilus influenzae infection in clinical test.


Assuntos
Cromatografia de Afinidade , Coloide de Ouro , Infecções por Haemophilus , Haemophilus influenzae , Animais , Cromatografia de Afinidade/instrumentação , Testes Diagnósticos de Rotina/normas , Coloide de Ouro/química , Infecções por Haemophilus/diagnóstico , Humanos , Limite de Detecção , Coelhos , Sensibilidade e Especificidade
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