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1.
Adv Exp Med Biol ; 1232: 393-399, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31893436

RESUMO

Although the existence of the primo vasculature system has been shown in many species, including mice, rats, rabbits and humans, the biological role of this system, including expression of genes and proteins, has not yet been investigated. Especially the transcriptional action by mRNA, which is required for biological action, needs to be studied in primo vasculature biology. Differentially expressed genes in both isolated primo vessels and lymphatic vessels of rabbits were analyzed by RNA sequencing experiments. Primer efficiency and RNA purity of the primo vessels under lipopolysaccharides were confirmed prior to performing real-time qRT-PCR analysis following RNA extraction. We demonstrated that FLT4 was enriched in primo vessels and that several genes, including HSPH1 and EPHB2, were highly expressed in primo vessels compared with lymphatic vessels. Our data show that almost all genes, except HSPA4, were increased or sustained in isolated primo vessels compared with lymphatic vessels (FLT4 2.58 fold, HSPH1 1.83 fold, EPHB2 1.52 fold; whereas HSPA4 decreased 0.50 fold), suggesting primo vessels as a central regulator in diverse physiology. This implies that FLT4, HSPH1, and EPHB2 in high amounts may be involved in the functional activity of primo vessels. Our experimental data show that several genes are highly enriched in primo vessels in the lymphatic vessels of the rabbit.


Assuntos
Regulação da Expressão Gênica , Vasos Linfáticos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Animais , Proteínas de Choque Térmico HSP110/genética , Vasos Linfáticos/metabolismo , RNA Mensageiro/genética , Coelhos , Receptor EphB2/genética , Análise de Sequência de RNA , Receptor 3 de Fatores de Crescimento do Endotélio Vascular/genética
2.
Food Chem ; 306: 125602, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31629969

RESUMO

Effects of high-pressure treatments (HPT, 100-300 MPa, 9 min, 25 °C) on the in vitro digestibility of gel-type meat products were studied using a simulated digestion-model. In vitro digestibilities of the cooked rabbit meat batters throughout the simulated oral-, gastric-, and intestinal-phases were determined. Peptides in the intestinal digesta were identified via Mass Spectrometer. Results revealed that in vitro digestibilities of HPT-samples were higher than the control (1.98%, 6.13% and 61.31% for oral-, gastric- and intestinal-phase respectively) throughout the digestion (P < 0.05). Alterations of the peptide profiles were induced by HPT, showing HPT-specific patterns of mutual peptides in the digestive products. Coupled with the identifications of salt-soluble proteins from raw batters, it was confirmed that myofibrillar proteins account for the major contribution to the HPT-induced changes. The results indicated that HPT can potentially be an effective technology to improve the digestibility of meat products.


Assuntos
Produtos da Carne/análise , Peptídeos/química , Animais , Culinária , Pressão , Proteólise , Coelhos
3.
Ann Otol Rhinol Laryngol ; 129(1): 46-54, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31466464

RESUMO

OBJECTIVES: To assess and compare the face (FV) and content validity (CV) of three ex vivo animal models for simulation training in pediatric laryngotracheal reconstruction (LTR). METHODS: Feasibility of performing LTR was assessed on the head and neck of three different animals (lamb/suckling-pig/rabbit) and laryngeal dimensions and qualitative observations were recorded. A 19-item five-point Likert scale questionnaire was completed for each model to assess FV and CV. Data was prospectively collected and analyzed using descriptive and nonparametric statistics. RESULTS: All three models were suitable for LTR simulation with laryngeal dimensions corresponding to 0-2 years (rabbit), 5-10 (pig) and >10 years (lamb model). Five trainees and five expert pediatric otolaryngologists performed LTR on each model. The overall median FV score was 5 for the lamb model (IQR 4-5), 3 for the rabbit (IQR 2-3), and 4 for the pig (IQR 4-4). The overall median CV score was 5 for the lamb (IQR 5-5), 2 for the rabbit (IQR 2-3), and 4 for the pig model (IQR 4-4). Comparison of the models demonstrated the lamb to be favored as the most realistic and practical model for simulation training in pediatric LTR, with both the lamb and the porcine model attaining validation thresholds. CONCLUSION: Our study is the first comparative validation assessment of animal models for use in pediatric LTR simulation and it supports the use of ex vivo lamb and porcine models for use in LTR surgical skills training. The lamb model was the favored simulation model while the rabbit was considered inferior for simulation training in pediatric LTR. LEVEL OF EVIDENCE: 3b.


Assuntos
Laringe/cirurgia , Procedimentos Cirúrgicos Otorrinolaringológicos/educação , Procedimentos Cirúrgicos Reconstrutivos/educação , Treinamento por Simulação , Traqueia/cirurgia , Animais , Criança , Pré-Escolar , Humanos , Lactente , Laringoestenose/cirurgia , Modelos Animais , Pediatria/educação , Coelhos , Ovinos , Suínos , Estenose Traqueal/cirurgia
4.
Med Oral Patol Oral Cir Bucal ; 25(1): e29-e33, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31880292

RESUMO

BACKGROUND: The aim of this research was to study anti-microbial and anti-inflammatory characteristics of silver nanoparticles helping bone structures to recover during late stage of parodontitis, which afterwards will increase the effect of bone regeneration operations. MATERIALS AND METHODS: We assessed colloid solution-derived silver nanoparticles coating of polylactic acid membrane regarding tissue foreign body response. Thirty eight polylactic acid membranes were implanted intracranially in rabbits - ten unmodified (control group) and twenty eight with silver nanoparticles coating (experimental group). In controls, penicillin was used for infection prophylaxis. Tissue response was assessed by light microscopy and immunohistochemistry (CD3, CD15, CD30) 2 weeks after implantation. RESULTS: inflammation markers in experimental group were significantly lower than in control group, there were no signs of forming a fibrosis capsule nor infectious signs. CONCLUSIONS: colloid silver solution can be used as a source of nanoparticles for anti-microbial and anti-inflammatory biodegradable membranes' coating.


Assuntos
Nanopartículas Metálicas , Prata , Animais , Regeneração Óssea , Poliésteres , Coelhos
5.
Zhongguo Zhong Yao Za Zhi ; 44(22): 4857-4863, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872593

RESUMO

To prepare Helix aspersa muller-paeonol nanogel( PAE-HAM-Gels) with anti-proliferative scar effect,evaluate its skin penetration,retention and irritation,and to investigate its prevention and treatment effect for hypertrophic scar in rabbit ears. The dermal retention,transdermal rate and cumulative permeability of paeonol were investigated in vitro by using the modified Franz diffusion cell and the abdominal skin of suckling pigs,SD rats and KM mice,respectively,and the in vitro permeation curves were drawn. The normal skin of the back of New Zealand rabbits was continuously treated with PAE-HAM-Gels for 7 days,and the physiological state of the skin was observed under light microscope after HE staining by using homologous left and right contrast method. The hypertrophic scar model in rabbit ears was established,and the New Zealand rabbits were randomly divided into blank group,model group,positive drug group,PAE-Gels group and PAE-HAM-Gels group. After 28 days of administration,the scar hyperplasia rate and scar elevation index( SEI) of each group were calculated; the scar tissues were taken and stained with Masson for observation of collagen fibers and muscle fibers hyperplasia under light microscope,and the expression level of TGF-ß1 in each group was detected. The Qnof PAE-HAM-Gels in aqueous solution was in line with the Higuchi equation,and its transdermal rate,cumulative permeation and dermal retention in different animal skins were all higher than those of PAE-Gels. The skin of the drug-administered group was intact,without erythema,edema or other phenomena; under light microscope,the subcutaneous tissue and the epidermal cells were neatly arranged with uniform thickness,which showed no difference from the blank group. The scar hyperplasia rate of the PAE-HAM-Gels group was 62. 50%; SEI was 2. 17±0. 33 and TGF-ß1 was( 815. 4±34. 69) ng·L~(-1),significantly different from those in model group( P<0. 01). Masson staining showed that as compared with the model group,the number of collagen fibers and muscle fibers was small and the arrangement was loose and tidy in the PAE-HAM-Gels group,with regular arrangement of chondrocytes and a small number of inflammatory cells and microvessels.PAE-HAM-Gels have good transdermal properties and dermal retention without skin irritation,offering a promising therapeutic strategy for transdermal delivery during the prevention and treatment of hypertrophic scar in rabbit ears.


Assuntos
Acetofenonas/química , Cicatriz Hipertrófica , Orelha , /química , Animais , Camundongos , Coelhos , Ratos , Ratos Sprague-Dawley , Suínos
6.
Zhonghua Yan Ke Za Zhi ; 55(12): 928-932, 2019 Dec 11.
Artigo em Chinês | MEDLINE | ID: mdl-31874507

RESUMO

Objective: Comparison of the permeability between the rabbit cornea and sclera ex vivo by determining the concentration of gatifloxacin using LC-MS/MS method, which may provide the basis for a new route of drug administration. Methods: Experimental study. The permeability of the cornea and sclera in healthy male New Zealand rabbits was evaluated by using Franz diffusion pool. We chose both gatifloxacin ophthalmic solution and gel as the test drugs, and calculated the cumulative permeation amounts (Qn), apparent permeability coefficient(P(app)). Results: The linear range of gatifloxacin was 5-1 000 ng/ml. The intra-day and inter-day precision was 1.7% -2.8% and 1.0% - 2.3%. Q(n) and P(app) of gatifloxacin ophthalmic solution in cornea and sclera ex vivo were 177.57, 517.52 µg/cm(2) and 4.34, 12.51 cm/s respectively, whereas that of gatifloxacin ophthalmic gel were 151.87, 411.05 µg/cm(2) and 3.66, 9.21 cm/s. Conclusion: This validated method could be applied to determine the gatifloxacin. The cumulative permeation amounts and apparent permeability coefficient of sclera are significantly higher than that of cornea for both ophthalmic solution and gel, suggesting that the development of a new route of drug administration based on sclera may have potential advantage. (Chin J Ophthalmol, 2019, 55: 928-932).


Assuntos
Esclera , Espectrometria de Massas em Tandem , Animais , Cromatografia Líquida , Córnea , Masculino , Permeabilidade , Coelhos
7.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 54(12): 912-918, 2019 Dec 07.
Artigo em Chinês | MEDLINE | ID: mdl-31887817

RESUMO

Objective: To establish a New Zealand rabbit animal model of laryngopharyngeal reflux disease (LPRD) using esophageal balloon together with metal internal stent dilation and to investigate the changes of mucosa. Methods: 20 New Zealand rabbits were randomly divided into experimental group and control group, with 10 in each group. Balloon dilatation and metal internal stent dilation were carried out in experimental group to reproduce the animal model of LPRD.The middle of balloon was placed at the lower esophageal sphincter (LES) while the stent was placed at the upper esophageal sphincter (UES). The guide wire was placed in the control group, but the balloon was not expanded and the stent was not placed. The general condition, pH value of hypopharynx, laryngeal histopathology and changes of pepsin content of New Zealand rabbits were observed regularly. The difference between experimental group and control group was compared. Results: The 24-hour Dx-pH monitoring results showed that the number of reflux episodes(20.0[9.5, 35.0], 13.0[6.5, 22.0]), and the percent time below pH 5.5 (1.36%[0.60%, 4.57%], 1.36%[0.43%, 2.77%]) in the experimental group at the 2nd and 4th week were significantly different from those in the control group (0[0,3.0], 1.0[0.5, 3.8]; 0[0, 0.01%], 0[0, 0], respectively, all P<0.01), suggesting that the experimental group New Zealand rabbits developed LPRD. Compared with the control group under microscope, lymphocytes infiltration and submucosal gland hyperplasia increased in the mucosa of the throat of the experimental group. The results of pepsin immunohistochemical staining between the two groups were statistically significant (P=0.014). Conclusion: The use of balloon dilatation of the LES combined with metal stent dilatation of the UES can successfully establish a laryngopharyngeal reflux model, and lesions in the throat tissue can be observed.


Assuntos
Modelos Animais de Doenças , Refluxo Laringofaríngeo , Laringe , Animais , Esfíncter Esofágico Inferior , Monitoramento do pH Esofágico , Laringe/fisiopatologia , Pepsina A , Coelhos
8.
Zhonghua Nei Ke Za Zhi ; 58(11): 808-813, 2019 Nov 01.
Artigo em Chinês | MEDLINE | ID: mdl-31665855

RESUMO

Objective: Positron emission tomography-computed tomography (PET-CT) has been used to quantify inflammatory response in the body. The aim of the present study was to explore the possibility of using this method to evaluate the stability of atherosclerotic plaques and the efficacy of atorvastatin in stabilizing atherosclerotic plaques. Methods: Twenty New Zealand male white rabbits were included and divided into the atorvastatin intervention group and the control group, with 10 rabbits in each group. Rabbits in both groups were fed with a high fat diet for 20 weeks, and treated with thoracoabdominal aortic balloon-pulling to establish atherosclerosis model at the end of the 2nd week. Rabbits in atorvastatin intervention group was given atorvastatin intragastrically once a day. At the 8th week, thoracoabdominal aortic ultrasound was used to detect plaques in all rabbits. Blood was drawn at the 3rd and the 20th week, respectively, to measure blood lipids, high-sensitive C-reactive protein (hs-CRP) and matrix metalloproteinase-9 (MMP-9). At the end of experiment, survival animals were scanned by (18)F-FDG PET-CT, and the average and maximum standard uptake values (SUVmean, SUVmax) of aortic segments were measured. Thereafter, the animals were sacrificed and aortic specimens of rabbits were taken and examined by immunohistochemistry. The pathological indexes were measured and compared. Results: At the end of experiment, the total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), hs-CRP [ (4.58±0.51) ng/ml vs.(5.87±0.66) ng/ml, P<0.01], MMP-9[ (43.93±2.16) ng/ml vs. (50.77±2.32) ng/ml, P<0.01], SUVmean (0.59±0.15 vs. 0.68±0.20, P<0.05) , SUVmax (0.68±0.20 vs. 0.81±0.27, P<0.05) , plaque area [ (0.36±0.24) mm(2) vs. (0.50±0.34) mm(2), P<0.05) ] and density of macrophage[ (4.34±1.54) % vs. (5.65±1.89) %, P<0.01] in the atorvastatin intervention group were significantly lower than those in the control group. In contrast, fiber cap thickness of the plaque[ (4.12±0.66) µm vs. (2.96±0.37) µm, P<0.01] in the atorvastatin intervention group was higher than that of the control group, and the difference was statistically significant. The arterial plaque areas were positively correlated with SUVmean (r=0.27, P<0.05) and SUVmax (r=0.43, P<0.01) . Fiber cap thickness was negatively correlated with SUVmean (r=-0.38, P<0.05) and SUVmax (r=-0.47, P<0.01) . The density of macrophage were positively correlated with SUVmean (r=0.52, P<0.01) and SUVmax (r=0.51, P<0.01) . Conclusion: (18)F-FDG PET/CT can be used to evaluate the efficacy of atorvastatin by the stability of atherosclerotic plaques.


Assuntos
Aorta/diagnóstico por imagem , Fluordesoxiglucose F18/administração & dosagem , Placa Aterosclerótica/diagnóstico por imagem , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons/métodos , Animais , Aorta/patologia , Masculino , Placa Aterosclerótica/patologia , Coelhos , Compostos Radiofarmacêuticos
9.
Georgian Med News ; (294): 136-141, 2019 Sep.
Artigo em Russo | MEDLINE | ID: mdl-31687966

RESUMO

The purpose of this study was to identify the features of skeletal muscle macrophagocytic submicroscopic architectonics, the dynamics of transformations of organelles and intracellular membranes at different time after the simulated gunshot wound. The study involved 26 breeding rabbits. Gunshot wounds were inflicted from a Fort 12 pistol. Rabbits were removed from the experiment on the 30th and 60th day, after the application of wounds. Ultrathin sections, after contrasting with lead citrate, were examined under an EM-125 electron microscope at an accelerating voltage of 75 kV, with an increase of 42,000-72,000. Electronic microscopic studies of macrophagocyte organelles taken from the scar tissue of the wound channel on the thirtieth day after a gunshot wound revealed a large number of macrophage cells that are in a state of high functional activity, dystrophic and destructive changes of organelles with focal disruption of the membrane of the nucleus, mitochondria and the granular endoplasmatic reticulum reticulation. The course of catabolic processes caused by the presence in the wound channel of toxic components, destroyed myosymplasts and heavy metal ions of the damaging element. On the sixtieth day after a gunshot wound, the increased activity of macrophage cells remained, due to sluggish current processes of utilization of the destroyed ultrastructural components of myosymplasts and heavy metal ions from the damaging element.


Assuntos
Macrófagos/patologia , Cicatrização , Ferimentos por Arma de Fogo , Animais , Microscopia Eletrônica de Varredura , Coelhos , Cicatrização/fisiologia
10.
Stomatologiia (Mosk) ; 98(5): 11-14, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31701923

RESUMO

AIM: To evaluate and to compare the intensity of the inflammatory response to zinc-oxide-eugenol based filling material in rabbit's soft and bone tissue. MATERIAL AND METHODS: The study employed 20 chinchilla rabbits. The artificial defects were formed in jaw and in soft tissues and filled by zinc-oxide-eugenol based filling material. After 14 or 30 days the animals were euthanized, and tissue samples were analyzed with microscope. RESULTS: Histological analysis revealed that zinc-oxide-eugenol based filling material support inflammation reaction in the tissue, but bone tissue showed statistically lower inflammatory response in comparison to soft tissue (p<0,05). CONCLUSION: Extrusion of zinc-oxide-eugenol based filling material from root canal is undesirable as it leads to complex unfavorable tissue reaction for its elimination, more pronounced in soft tissues.


Assuntos
Eugenol , Materiais Restauradores do Canal Radicular , Animais , Óxidos , Coelhos , Zinco , Cimento de Óxido de Zinco e Eugenol
11.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 463-467, 2019 Sep 27.
Artigo em Chinês | MEDLINE | ID: mdl-31713372

RESUMO

OBJECTIVE: To understand the situation of Schistosoma japonicum infections in wild animals in transmission-controlled schistosomiasis-endemic areas in Jiangxi Province, so as to provide scientific evidence for implementing precision control interventions and achieving the goal of transmission interruption and elimination of schistosomiasis. METHODS: Five endemic villages from Ruichang City and Pengze County that were heavily endemic for schistosomiasis in Jiangxi Province, were selected as the study villages. Wild animals like wild mice were captured, and the livers of wild animals were purchased from the snail habitats in the study villages for detection of S. japonicum infections. In the study villages, S. japonicum human infections were screened using indirect hemagglutination assay (IHA) followed by parasitological examinations with miracidial hatching test and Kato-Katz method, and the S. japonicum infection in livestock was tested using a miracidial hatching test with a plastic tube. In addition, snail survey was conducted in the study villages by means of systematic sampling combined with environmental sampling, and the S. japonicum infection in snails was detected using a loop-mediated isothermal amplification (LAMP) assay. RESULTS: A total of 240 liver specimens were sampled or purchased from 5 species of wild animals in the study villages, including wild mice, weasels, pigs, deer and rabbits. A total of 172 wild mice were captured, with a 2.91% rate of S. japonicum infection, and there was no S. japonicum infection detected in other wild animals. The prevalence of Capillaria hepatica infection was 12.21%, 1.96% and 12.50% in wild mice, deer and pigs, respectively. In addition, there was no S. japonicum infection found in either humans or livestock in the study villages, and the mean snail density varied from 0.13 to 0.80 snails/0.1 m2 in the study villages. LAMP assay detected S. japonicum infection in 2 tubes in a study village. CONCLUSIONS: The role of wild animals in schistosomiasis transmission and their potential risks can not be neglected in hilly schistosomiasis-endemic areas of Jiangsu Province after transmission control. Intensified surveillance and targeted control measures should be implemented to consolidate schistosomiasis control achievements.


Assuntos
Animais Selvagens , Schistosoma japonicum , Esquistossomose Japônica , Animais , Animais Selvagens/parasitologia , China/epidemiologia , Humanos , Fígado/parasitologia , Camundongos , Densidade Demográfica , Prevalência , Coelhos , Esquistossomose Japônica/epidemiologia , Esquistossomose Japônica/prevenção & controle , Caramujos/fisiologia , Inquéritos e Questionários , Suínos
12.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 35(9): 789-793, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31750819

RESUMO

Objective To develop the colloidal gold immunochromatography test strip for qualitatively detecting the hemoglobin (Hb) in human feces. Methods Mouse anti-human Hb monoclonal antibody SPR-5 marked by colloidal gold was coated in glass fiber membrane, and then the mouse anti-human Hb SP-5 monoclonal antibody and goat anti-mouse IgG were immobilized in testing (T) line and control (C) line located in nitrocellulose membranes, respectively. With this double antibody sandwich technique and immunochromatography test, the Hb antigen would react with both antibodies coated in the T line and C line and cause two colour reactions if the detected sample was positive, whereas the antigen-antibody combination and colour reaction only showed up in the C line when the sample was negative. Results The minimum detection limit of this test strip for human Hb was 21 ng/mL and no cross reactions were found in chick Hb, rabbit Hb, sheep Hb, pig Hb and cow Hb. Conclusion The test strips can improve the detection rate of fecal occult blood obviously and avoid false-positive results.


Assuntos
Cromatografia de Afinidade , Coloide de Ouro , Fitas Reagentes , Animais , Anticorpos Monoclonais , Bovinos , Reações Cruzadas , Feminino , Humanos , Sangue Oculto , Coelhos , Sensibilidade e Especificidade , Ovinos , Especificidade da Espécie , Suínos
13.
J Environ Pathol Toxicol Oncol ; 38(3): 271-283, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31679313

RESUMO

Certain mechanical stimuli-particularly low-magnitude, high-frequency vibration-could induce bone marrow stem cell osteogenic differentiation and promote bone formation via Wnt signaling pathway, although the molecular mechanism is still unclear. In this study, we found that miR-335-5p is significantly upregulated after low-magnitude, high-frequency vibration, which suppresses the expression of the Wnt signaling inhibitor Dickkopf-related protein 1. Inhibition of miR-335-5p greatly reduced the osteogenic differentiation. Furthermore, the increase of miR-335-5p level was also confirmed in vivo after LMHF vibration in rabbit. Our study elucidates the prominent role of miRNAs that links the LMHF vibration and osteogenic differentiation.


Assuntos
Expressão Gênica , Células-Tronco Mesenquimais/metabolismo , MicroRNAs/genética , Osteogênese/fisiologia , Vibração/efeitos adversos , Animais , Diferenciação Celular/fisiologia , Masculino , MicroRNAs/metabolismo , Coelhos
14.
Zhonghua Xin Xue Guan Bing Za Zhi ; 47(11): 887-893, 2019 Nov 24.
Artigo em Chinês | MEDLINE | ID: mdl-31744278

RESUMO

Objective: To investigate the effect of sacubitril/valsartan on cardiac function in heart failure rabbits with preserved ejection fraction. Methods: Forty-five healthy adult male New Zealand rabbits were divided into sham operation group (n=12) and model group (n=33) by random number table method. HFpEF model was constructed by abdominal aortic constriction in model group. In sham operation group, 1 rabbit died due to anesthesia accident, and 1 rabbit in model group died of acute left heart failure. At 8 weeks of modeling, 3 rabbits were excluded due to the failure to establish the successful model. At the 8th week of modeling, 2 rabbits in sham operation group were selected and sacrificed by random number table method, and 3 rabbits in model group were selected and sacrificed for myocardial histological examination. Then, 9 rabbits in sham operation group and 26 rabbits in model group entered the subsequent experiment. The model group was randomly divided into untreated group (n=8), valsartan intervention group (n=9), and sacubitril/valsartan intervention group (n=9), respectively, drugs were applied per gavage. The feeding and exercise activity of rabbits in each group were evaluated by simple cardiac function classification at baseline, 4 and 8 weeks post intervention. Echocardiography was used to detect interventricular septal thickness at diastole(IVSd), interventricular septal thickness at systolic(IVSs), left ventricular posterior wall of diastolic(LVPWd), left ventricular internal diameter at diastolic(LVIDd), left ventricular internal diameter at systolic(LVIDs), and calculate the left ventricular ejection fraction(LVEF), mitral valve's early diastolic flow velocity(E)/late mitral diastolic maximum flow rate ratio(A) and heart rate at baseline, 4 and 8 weeks post intervention. Serum N terminal B-type natriuretic peptide (NT-proBNP) and angiotensin (Ang)Ⅱ and soluble matrix lysin 2(sST2) content was determined by ELISA at baseline, 4 and 8 weeks post intervention. Eight weeks after intervention, the hearts of rabbits were taken and weighed, and heart mass index (HMI) and left ventricular mass index (LVMI) were calculated. Results: (1) Evaluation results of cardiac function: there were 2, 5, and 2 rabbits with cardiac function grade Ⅰ,Ⅱ and Ⅲ before the drug intervention, and 4, 4, and 1 rabbits with respective cardiac function grade after 8 weeks of intervention in valsartan group (P>0.05). There were 2, 4, and 3 rabbits with heart function gradeⅠ,Ⅱ and Ⅲ before the drug intervention, and 7, 2, and 0 rabbits with respective heart function grade after 8 weeks of intervention in sacubitril/valsartan group(P<0.05). (2) Echocardiographic results: at 8 weeks after drug intervention, IVSd and IVSs of rabbits in untreated group were significantly higher than those in sham operation group, and the ratio of E/A was significantly lower than that in sham operation group(all P<0.01). IVSs of the valsartan group was significantly higher than that of sham operation group, and the ratio of E/A was significantly lower than that of sham operation group(all P<0.01). The E/A ratio in the sacubitril/valsartan group was significantly lower than that in sham operation group(P<0.01). IVSd and IVSs in valsartan group were significantly lower than those in untreated group(all P<0.05), and IVSd in sacubitril/valsartan group was significantly lower than that in untreated group(P<0.01). The IVSd, IVSs, LVPWd, LVIDd, LVIDs, LVEF, E/A ratios were similar between sacubitril/valsartan group and valsartan group(all P>0.05). There was no significant difference in heart rate between the groups(P>0.05). (3) Serum NT-proBNP, Ang Ⅱ and sST2 levels: 4 weeks after drug intervention, untreated group, valsartan group, and sacubitril/valsartan group's serum NT-proBNP levels were significantly higher than that of sham operation group(all P<0.01); serum NT-proBNP was significantly lower in sacubitril/valsartan group than that in untreated group(P<0.01). Four weeks after intervention, serum AngⅡ levels were significantly higher in untreated group, valsartan group, sacubitril/valsartan group than in sham group(all P<0.01), but there was no statistically significant difference between the modeling groups(P>0.05). Four weeks after drug intervention, the serum sST2 contents in untreated group, valsartan group, and sacubitril/valsartan group were significantly higher than in sham operation group(all P<0.01), and which was significantly lower in valsartan group and sacubitril/valsartan group than in untreated group(all P<0.01), which were significantly lower in sacubitril/valsartan group than in valsartan group(P<0.01). Eight weeks after drug intervention, serum NT-proBNP levels were significantly higher in untreated group, valsartan group, and sacubitril/valsartan group than in sham operation group(all P<0.01), which were significantly lower in valsartan group and sacubitril/valsartan group than in untreated group(all P<0.01), which were significantly lower in valsartan group than in sacubitril/valsartan group(P<0.01). Eight weeks after drug intervention, Ang Ⅱ levels were significantly higher in valsartan group and sacubitril/valsartan group than in untreated group(all P<0.01), which tended to be higher in untreated group and valsartan group, tended to be lower in sacubitril/valsartan compared to value at 4 weeks(all P>0.05). Eight weeks after drug intervention, serum sST2 was significantly higher in untreated group and valsartan group than in sham operation group(all P<0.01), which tended to be higher in sacubitril/valsartan group compared to sham operation group(P>0.05), which were significantly lower in valsartan group and sacubitril/valsartan group than in untreated group(all P<0.01), which was significantly lower in sacubitril/valsartan group than in valsartan group(P<0.01). (4) Comparison of whole-heart mass, left ventricular mass, HMI and LVMI: 8 weeks after drug intervention, the whole-heart mass, left ventricular mass, HMI and LVMI were significantly higher in untreated group than in sham operation group(all P<0.01), and the above indexes were also significantly higher in valsartan group than in sham operation group(all P<0.05), tended to be lower in valsartan group compared to untreated group (all P>0.05). HMI and LVMI were lower in sacubitril/valsartan group than in untreated group(all P<0.05). All the above indexes tended to be lower in sacubitril/valsartan group than in valsartan group(all P>0.05). Conclusion: Sacubitril/valsartan is superior to valsartan alone on improving cardiac function in HFpEF rabbits.


Assuntos
Insuficiência Cardíaca , Aminobutiratos , Animais , Masculino , Coelhos , Volume Sistólico , Tetrazóis , Valsartana
15.
Beijing Da Xue Xue Bao Yi Xue Ban ; 51(5): 887-892, 2019 Oct 18.
Artigo em Chinês | MEDLINE | ID: mdl-31624394

RESUMO

OBJECTIVE: To evaluate the barrier effect of an absorbable barrier membrane made by small intestinal submucosa (SIS) compared with Bio-Gide collagen membrane. METHODS: 12 healthy New Zealand male white rabbits were randomly assigned. A or B round bone defects with a depth of 2 mm and a diameter of 5 mm or 8 mm was made in each rabbit's mandibular. The following treatments were given respectively: covered with SIS membrane (S), covered with Bio-Gide membrane (G) and blank control (O). Then we got six groups: AS, AG, AO, BS, BG, and BO (n=4). After 4 weeks, the rabbits were sacrificed. The specimens were examined by naked-eye observation, new bone percentage (BV/TV) and bone mineralized density (BMD), which were measured and analyzed by Micro-CT. The data were analyzed with one-way ANOVA. RESULTS: After 4 weeks, Bio-Gide membranes were fused with the surrounding tissue while SIS membranes held the form with no significant degradation. In the AS, BS and AG groups, the absorbable membranes smoothly covered on the new bone. While in the BG group, Bio-Gide membranes collapsed to the center of the bone defects. The 3D reconstruction of Micro-CT showed that a large number of newly formed trabeculae were found in the four groups of AS, BS, AG, and BG. In the central subsidence area of the BG group, the newly formed trabeculae were sparse. However only a small amount of new bone trabecula appeared at the bottom of the defects in groups AO and BO. Micro-CT quantitative results showed that BV/TV (39.10%±0.79%) and BMD [(517.73±11.22) mg/cm3] of AS group were significantly higher than those of AO group [26.67%±1.12%, (319.81±8.00) mg/cm3] (P<0.05), and there was no significant difference between AS group and AG group [38.15%±0.91%, (518.65±7.48) mg/cm3] (P>0.05). BV/TV (34.90%±1.35%) and BMD [(409.09±8.14) mg/cm3] of BS group were significantly higher than those of BO group [23.63%±2.07%, (171.00±16.24) mg/cm3] (P < 0.05). Meanwhile, there was no significant difference between BS and BG groups [33.40%±1.06%, (412.70±8.6) mg/cm3] (P>0.05). HE staining analysis revealed that significant bone formation was achieved in the AS, AG, BS and BG groups, and trabecular bone of AS and AG groups were thicker and denser. In AO and BO group, there were scattered new bone tissues in edges of host bone, and no coarse trabecular bone formed. CONCLUSION: In the early healing of two sizes bone defects in rabbit mandibular, SIS membrane and Bio-Gide membrane have a similar barrier effect in guided bone regeneration. And SIS membrane's ability to maintain space for bone regeneration seems to be better.


Assuntos
Regeneração Óssea , Mandíbula , Implantes Absorvíveis , Animais , Masculino , Osteogênese , Coelhos , Distribuição Aleatória , Suínos , Cicatrização , Microtomografia por Raio-X
16.
Int J Periodontics Restorative Dent ; 39(6): e219-e227, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31613950

RESUMO

Several methods are used for periodontal regeneration and alveolar ridge reconstruction. This study aimed to compare the bone regeneration capacities of MinerOss X, Bio-Oss, and autograft in rabbit calvaria. In this animal study, four defects measuring 8 mm in diameter were created in the calvaria of 10 rabbits using a trephine bur. Three defects were randomly filled with MinerOss X, Bio-Oss, and autogenous bone. The fourth defect remained empty as a control. The rabbits were sacrificed after 4 or 8 weeks. Calvarial samples were taken and histopathologically evaluated. The mean number of osteoblasts and the amount of newly formed bone in all groups were higher at 8 compared to 4 weeks. At 4 and 8 weeks, the amount of newly formed bone and number of osteoblasts and osteocytes in MinerOss X group were significantly greater than the corresponding values in other groups, and MinerOss X was superior to Bio-Oss and autograft in all the assessed variables at both 4 and 8 weeks (P < .001). Within the limitations of this study, the results showed that the number of osteoblasts and osteocytes and the amount of newly formed bone in the MinerOss X group were higher than those in Bio-Oss and autograft groups. However, further clinical studies are warranted.


Assuntos
Substitutos Ósseos , Animais , Regeneração Óssea , Minerais , Coelhos
17.
Zhongguo Gu Shang ; 32(9): 853-860, 2019 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-31615185

RESUMO

OBJECTIVE: To explore the effect of lentivirus-mediated BMP-2 overexpression plasmid transfection into bone marrow mesenchymal stem cells and silk fibroin scaffold on osteoblast transformation. METHODS: The lentivirus BMP-2 overexpression vector was constructed, bone marrow mesenchymal stem cells were cultured, and the combined culture system of nuclear scaffolds was constructed. Alizarin red staining and alkaline phosphatase staining were used to detect the osteogenic transformation of bone marrow mesenchymal stem cells in vitro. Ten New Zealand white rabbits, weighing 3.2 to 4.5 kg(averaging 3.9 kg), aged (2.89±0.45) years old, were selected to construct the rabbit tibial defect model by drilling a conical tibial defect (5 mm in length, 2 mm in width and 3 mm in depth) with an oral drill. The repair of the tibial defect in the animal model was observed by HE staining. The experimental group was implanted with silk fibroin scaffold + BMP-2 overexpression vector bone marrow mesenchymal stem cell complex, while the negative control group was implanted with silk fibroin scaffold+non-transfected bone marrow mesenchymal stem cell complex. RESULTS: Compared with the control group(silk fibroin scaffold+non-transfected bone marrow mesenchymal stem cells), the number of adherent cells on the surface of the scaffold in the experimental group(silk fibroin scaffold+transfected BMP-2 overexpression vector BMP-2 complex) increased significantly. Compared with the control group, the ECM secretion in the experimental group increased significantly. EDX analysis showed that the content of calcium ion was 0.22% in the control group and 0.86% in the experimental group, which showed that the ability of inducing calcium ion formation in the experimental group was stronger than that in the control group. Alizarin red staining of calcium nodules showed that there was no obvious change in the naked eye of the control group, and a small amount of calcium nodules could be seen under the microscope. In the experimental group, obvious red area staining was observed by naked eye, and a large number of calcium nodules were observed by microscopy. The results of alkaline phosphatase staining showed that there was no obvious change in the naked eye of the control group, and no obvious change in the microscopic observation. In the experimental group, purple area staining was observed by naked eyes, and ALP staining was strongly positive by microscopy. The combined culture system of silk fibroin scaffold and bone marrow mesenchymal stem cells can repair cartilage defects. The repair effect of BMP-2 bone marrow mesenchymal stem cells after transfection is obviously better than that of non-transfection group. HE staining showed that inflammatory cells decreased and scaffolds disappeared slightly in the control group. In the experimental group, inflammatory cells were significantly reduced, scaffolds disappeared and angiogenesis was observed. CONCLUSIONS: Lentivirus-mediated BMP-2 overexpression plasmid can promote BMSC to differentiate into osteocytes and secrete more extracellular matrix containing Ca²âº to promote bone defect repair.


Assuntos
Células-Tronco Mesenquimais , Animais , Células da Medula Óssea , Proteína Morfogenética Óssea 2 , Células Cultivadas , Fibroínas , Lentivirus , Osteoblastos , Osteogênese , Plasmídeos , Coelhos , Transfecção
18.
Ann Parasitol ; 65(3): 237-243, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31599539

RESUMO

Rabbits are commonly reared by households and farmers in Nigeria as a source of meat, but there is no information available on Cryptosporidium genotypes occurring in rabbits in Nigeria. Fecal samples were collected from 107 rabbits and examined by modified Ziehl-Neelsen technique for the presence of Cryptosporidium oocysts. An infection rate of 3.7% (4/107) was obtained and all microscopy-positive samples were genotyped and subtyped to determine the circulating Cryptosporidium species using sequence analysis of the 18S rRNA gene and 60-kDa glycoprotein (gp60) gene, respectively. All the four microscopy-positive samples were identified as C. parvum by 18S rRNA gene. However, analysis of the gp60 gene revealed the presence of C. parvum subtype IIc, which is commonly found in humans in two isolates. These findings indicate natural infection of rabbits with C. parvum and underscore the need to investigate the probable role of animal hosts in the epidemiology of Cryptosporidium infection. This is the first report on genetic characterization of Cryptosporidium infecting rabbits in Nigeria.


Assuntos
Criptosporidiose , Cryptosporidium parvum , Doenças Parasitárias em Animais , Animais , Criptosporidiose/diagnóstico , Criptosporidiose/epidemiologia , Criptosporidiose/parasitologia , Cryptosporidium parvum/genética , Fezes/parasitologia , Genótipo , Humanos , Nigéria/epidemiologia , Doenças Parasitárias em Animais/diagnóstico , Doenças Parasitárias em Animais/epidemiologia , Doenças Parasitárias em Animais/parasitologia , Reação em Cadeia da Polimerase , RNA Ribossômico 18S/genética , Coelhos , Análise de Sequência de DNA , Sialoglicoproteínas/genética
19.
Ann Parasitol ; 65(3): 267-273, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31599543

RESUMO

Coccidiosis remains a serious concern of great economic impact on domestic animals including rabbits. This study was conducted to determine the prevalence, species diversity, and associated risk factors of rabbit coccidiosis in Ilorin, Kwara State Nigeria. Fecal samples were examined using the floatation technique (positive samples were sporulated for identification of the different Eimeria species). 169 out of 215 (78.6%,) rabbits were found to be infected with Eimeria species. A total of seven Eimeria species (Eimeria coecicola, Eimeria irresidua, Eimeria perforans, Eimeria magna, Eimeria intestinalis, Eimeria stiedai and Eimeria flavescens) were identified. Of these, Eimeria coecicola was the most prevalent (48/215; 22.3%), while E. flavescens (8/215; 3.7%) was the least prevalent. Weaners (80.0%) were more infected than bunnies (79.1%) and adult (77.5%). Females had a higher infection rate (79.4%) than males (77.4%). Californian breed were more infected (84.9%) compared to Chinchila (83.7%), Dutch breed (80.9%), and New Zealand White (63.6%). Rabbits kept in the deep litter housing system had higher prevalence rate (95.2%) compared to those raised in the battery cage system (71.9%). Coccidiosis was more prevalent during the wet season than the dry season. This study concludes that Eimeria infections of rabbit is endemic in Ilorin, Kwara State with breeds and housing type been significant risk factors associated with the infection. This study provides the baseline information as the first report on the different Eimeria species affecting rabbits in Nigeria.


Assuntos
Coccidiose , Eimeria , Doenças Parasitárias em Animais , Animais , Coccidiose/epidemiologia , Coccidiose/veterinária , Feminino , Masculino , Nigéria/epidemiologia , Doenças Parasitárias em Animais/epidemiologia , Prevalência , Coelhos , Fatores de Risco
20.
Vestn Oftalmol ; 135(4): 41-49, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31573556

RESUMO

PURPOSE: To investigate the clinical and morphological manifestations of ocular lesions resulting from acute exposure to microwave radiation (MR). MATERIAL AND METHODS: Twenty-four rabbits were included in the study and divided into four equal groups according to MR exposure time (15, 30, 45, 60 s). The right eyes of rabbits were exposed to MR of 3.97 GHz and energy density of 1.0 W/cm2. The sham control group consisted of six animals. The exposed (right) and the paired (left) eye were studied for clinical and morphological changes, content of proinflammatory cytokines IL-1ß, IL-6 and TNF-α in the anterior chamber and in the vitreous body after exposure to MR. RESULTS: Significant dose-dependent changes in the structure of the exposed eye were revealed. Formation of microwave cataract was noted at the MR exposure time of 15 seconds and more. Partial or complete de-epithelialization, stromal edema, endothelial damage and inflammatory infiltration in the cornea, effusion of protein and cellular reaction in the aqueous humor were detected after MR exposure of 30, 45 and 60 seconds. Cellular reaction in the vitreous body was observed after exposure time of 45 and 60 seconds. Exposure to MR for up to 1 minute did not lead to visible clinical or morphological (traditional methods of examination) damage of the retina and optic nerve within 24 hours. Significantly higher content of proinflammatory cytokines IL-1ß, IL-6 and TNF-α in the aqueous humor and vitreous body was revealed in animals exposed to MR for 45 and 60 seconds. CONCLUSION: Acute exposure of the organ of vision to electromagnetic microwave radiation can lead to adverse dose-dependent effects not only in the lens, but also in other structures of the eye.


Assuntos
Catarata , Cristalino , Animais , Humor Aquoso , Radiação Eletromagnética , Coelhos , Corpo Vítreo
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