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1.
J Sci Food Agric ; 102(2): 584-596, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34159603

RESUMO

BACKGROUND: The loss of coffee leaves caused by the attack of pests and diseases significantly reduces its production and bean quality. Thus this study aimed to estimate foliation for regions with the highest production of arabica coffee in Brazil using nonlinear models as a function of climate. A 25-year historical series (1995-2019) of Coffea arabica foliation (%) data was obtained by the Procafé Foundation in cultivations with no phytosanitary treatment. The climate data were obtained on a daily scale by NASA/POWER platform with a temporal resolution of 33 years (1987-2019) and a spatial resolution of approximately 106 km, thus allowing the calculation of the reference evapotranspiration (PET). Foliation estimation models were adjusted through regression analysis using four-parameter sigmoidal logistic models. The analysis of the foliation trend of coffee plantations was carried out from degrees-day for 70 locations. RESULTS: The general model calibrated to estimate the arabica coffee foliation was accurate (mean absolute percentage error = 2.19%) and precise (R2 adj  = 0.99) and can be used to assist decision-making by coffee growers. The model had a sigmoidal trend of reduction, with parameters ymax  = 97.63%, ymin  = 9%, Xo  = 3517.41 DD, and p = 6.27%, showing that foliation could reach 0.009% if the necessary phytosanitary controls are not carried out. CONCLUSION: Locations with high air temperatures over the year had low arabica coffee foliation, as shown by the correlation of -0.94. Therefore, coffee foliation can be estimated using degree days with accuracy and precision through the air temperature. This represents great convenience because crop foliation can be obtained using only a thermometer. © 2021 Society of Chemical Industry.


Assuntos
Coffea/crescimento & desenvolvimento , Folhas de Planta/crescimento & desenvolvimento , Brasil , Mudança Climática , Coffea/química , Temperatura Alta , Dinâmica não Linear , Folhas de Planta/química
2.
Food Chem ; 367: 130679, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34352695

RESUMO

The chemical complexity of coffee influences the sensory evaluation of the beverage, the main method used to define the quality of the coffee. In view of the subjectivity that method offers, we propose the association of an instrumental method with multivariate calibration (PLS and GA-SVR) to predict the quality of arabica coffee as support for sensory analysis. Arabica coffee samples were submitted to sensory evaluation using the Specialty Coffee Association (SCA) protocol and HS-SPME-GC/MS analysis. The models presented RMSEp results from 0.20 to 0.25, within the evaluation range the quality levels of sensory attributes (0.25). For the fragrance/aroma attribute, a value of R2p equal to 0.8503 was reached. 15 volatile compounds were identified as responsible for predicting the quality of arabica coffee, among which, 1-nonadecene was first reported as an impact compound in the prediction of important sensory attributes.


Assuntos
Coffea , Café , Calibragem , Cromatografia Gasosa-Espectrometria de Massas , Odorantes/análise
3.
Food Chem ; 366: 130504, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34273856

RESUMO

Drying process affected the qualitative indicators of green coffees; chlorogenic acid (CGAs), total phenolic content (TPC), antioxidant activities and CIE-lab color to varying degrees. Sun drying and heat pump drying resulted in comparable levels of CGAs and antioxidant activities in green coffees; however, color parameters, especially lightness (L*), differed. Correlation analyses indicated a relationship between specific CGAs, antioxidant activities and color parameters among coffees. PLS analysis revealed that the high contents of 5-caffeoylquinic acid in green coffees did not correlate with antioxidant activities. Results from CGAs contents and PCA analysis provided a linkage to previous research relating important components and quality indices of both green and roasted coffees as affected by postharvest drying. Results indicated that heat pump drying at 50 °C is a viable alternative and possibly superior to sun drying for preserving certain desirable chemical and physical characteristics of green coffee.


Assuntos
Ácido Clorogênico , Coffea , Antioxidantes , Ácido Clorogênico/análise , Café , Tailândia
4.
PLoS One ; 16(12): e0260499, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34905539

RESUMO

The coffee berry borer (CBB) Hypothenemus hampei Ferrari is the most serious pest of coffee worldwide. Management of the CBB is extremely difficult because its entire life cycle occurs inside the fruit, where it is well protected. Knowing which life stages contribute most to population growth, would shed light on the population dynamics of this pest and help to improve CBB management programs. Two staged-classified matrices were constructed for CBB populations reared in the lab on artificial diets and CBB populations from artificial infestations in the field. Matrices were used to determine demographic parameters, to conduct elasticity analyses, and to perform prospective perturbation analysis. Higher values of the intrinsic rate of natural increase (rm) and population growth rate (λ): were observed for CBB populations growing in the lab than in the field (rm: 0.058, λ: 1.74 lab; rm: 0.053, λ: 1.32 field). Sensitivity values for both CBB populations were highest for the transitions from larva to pupa (G2: 0.316 lab, 0.352 field), transition from pupa to juvenile (G3: 0.345 lab, 0.515 field) and survival of adult females (P5: 0.324 lab, 0.389 field); these three vital rates can be important targets for CBB management. Prospective perturbation analyses indicated that an effective management for the CBB should consider multiple developmental stages; perturbations of >90% for each transition are necessary to reduce λ to <1. However, when the three vital rates with highest sensitivity are impacted at the same time, the percentage of perturbation is reduced to 25% for each transition; with these reductions in survival of larvae, pupae and adult females the value of λ was reduced from 1.32 to 0.96. Management programs for CBB should be focused on the use of biological and cultural measures that are known to affect these three important targets.


Assuntos
Coffea/parasitologia , Frutas/parasitologia , Larva/fisiologia , Controle Biológico de Vetores/métodos , Pupa/fisiologia , Gorgulhos/fisiologia , Animais , Feminino , Fertilidade/fisiologia , Longevidade/fisiologia , Masculino , Dinâmica Populacional/estatística & dados numéricos , Porto Rico
5.
PLoS One ; 16(12): e0260997, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34965248

RESUMO

Breeding programs of the species Coffea canephora rely heavily on the significant genetic variability between and within its two varietal groups (conilon and robusta). The use of hybrid families and individuals has been less common. The objectives of this study were to evaluate parents and families from the populations of conilon, robusta, and its hybrids and to define the best breeding and selection strategies for productivity and disease resistance traits. As such, 71 conilon clones, 56 robusta clones, and 20 hybrid families were evaluated over several years for the following traits: vegetative vigor, incidence of rust and cercosporiosis, fruit ripening time, fruit size, plant height, canopy diameter, and yield per plant. Components of variance and genetic parameters were estimated via residual maximum likelihood (REML) and genotypic values were predicted via best linear unbiased prediction (BLUP). Genetic variability among parents (clones) and hybrid families was detected for most of the evaluated traits. The Mulamba-Rank index suggests potential gains up to 17% for the genotypic aggregate of traits in the hybrid population. An intrapopulation recurrent selection within the hybrid population would be the best breeding strategy because the genetic variability, narrow and broad senses heritabilities and selective accuracies for important traits were maximized in the crossed population. Besides, such strategy is simple, low cost and quicker than the concurrent reciprocal recurrent selection in the two parental populations, and this maximizes the genetic gain for unit of time.


Assuntos
Coffea/genética , Resistência à Doença/genética , Hibridização Genética , Melhoramento Vegetal , Doenças das Plantas/genética , Característica Quantitativa Herdável , Meio Ambiente , Genótipo , Funções Verossimilhança
6.
Plant Sci ; 312: 111018, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34620453

RESUMO

The genus Coffea (Rubiaceae) encompasses a group of perennial plant species, including a commodity crop from which seeds are roasted, ground, and infused to make one of the most appreciated beverages in the world. As an important tropical crop restricted to specific regions of the world, coffee production is highly susceptible to the effects of environmental instabilities (i.e., local year-to-year weather fluctuations and global climate change) and threatening pest pressures, not to mention an increasing quality rigor by consumers in industrialized countries. Specialized metabolites are substances that largely affect plant-environment interactions as well as how consumers experience agricultural products. Membrane transporters are key targets, albeit understudied, for understanding and tailoring the spatiotemporal distribution of specialized metabolites as they mediate and control molecular trafficking and substance accumulation. Therefore, we analyzed the transportome of C. canephora encoded within the 25,574 protein-coding genes annotated in the genome of this species and identified 1847 putative membrane transporters. Following, we mined 152 transcriptional profiles of C. canephora and C. arabica and performed a comprehensive co-expression analysis to identify transporters potentially involved in the accumulation of specialized metabolites associated with beverage quality and bioactivity attributes. In toto, this report points to an avenue of possibilities on Coffea genomic and transcriptomic data mining for genetic breeding strategies, which can lead to the development of new, resilient varieties for more sustainable coffee production systems.


Assuntos
Cafeína/metabolismo , Ácido Clorogênico/metabolismo , Coffea/genética , Coffea/metabolismo , Proteínas de Membrana Transportadoras/genética , Sementes/genética , Sementes/metabolismo , Cafeína/genética , Produtos Agrícolas/genética , Produtos Agrícolas/metabolismo , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Genômica , Transcriptoma
7.
Molecules ; 26(20)2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34684776

RESUMO

Oxidative stress in aquatic organisms might suppress the immune system and propagate infectious diseases. This study aimed to investigate the protective effect of polyphenolic extracts from spent coffee grounds (SCG) against oxidative stress, induced by H2O2, in C. viridis brain cells, through an in vitro model. Hydrophilic extracts from SCG are rich in quinic, ferulic and caffeic acids and showed antioxidant capacity in DPPH, ORAC and FRAP assays. Furthermore, pretreatment of C. viridis brain cells with the polyphenolic extracts from SCG (230 and 460 µg/mL) for 24 h prior to 100 µM H2O2 exposure (1 h) significantly increased antioxidant enzymes activity (superoxide dismutase and catalase) and reduced lipid peroxidation (measured by MDA levels). These results suggest that polyphenols found in SCG extracts exert an antioxidative protective effect against oxidative stress in C. viridis brain cells by stimulating the activity of SOD and CAT.


Assuntos
Antioxidantes/química , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Café/química , Perciformes/metabolismo , Animais , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Encéfalo/citologia , Catalase/metabolismo , Células Cultivadas , Coffea/química , Proteínas de Peixes/metabolismo , Pesqueiros , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/isolamento & purificação , Sequestradores de Radicais Livres/farmacologia , Peróxido de Hidrogênio/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Polifenóis , Superóxido Dismutase/metabolismo
8.
Exp Appl Acarol ; 85(1): 1-17, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34581908

RESUMO

Tenuipalpid mites of the genus Brevipalpus are of significant economic and quarantine importance in agriculture. They can damage and vector phytopathogenic viruses in coffee plantations and other crops. In this study, we focused on: identification of the Brevipalpus species, assessment of the spread of Brevipalpus-associated viruses (CoRSV, CiLV-N, CiLVC and CiLVC2), and mite population fluctuations over the course of 1 year. The study was conducted in coffee plantations in Soconusco, a coffee-producing region in Chiapas, Mexico. The collected mites of the Brevipalpus phoenicis sensu lato species complex (635) were identified as Brevipalpus papayensis (80.2%) and B. yothersi (19.8%) based on morphological and molecular characteristics. Their population abundance was low and there were no indications for virosis. The highest mite abundance was recorded in August-September and the lowest in February-March. An interaction was observed between mite abundance and coffee species in open-growth and shaded cultivation at various altitudes. Brevipalpus papayensis was most abundant in Coffea arabica var. Bourbon, in shaded (80%) growing conditions at an altitude of 1300 m above sea level. In C. canephora (in open-growth cultivation conditions at low altitude), B. yothersi was more abundant than in C. arabica, and as abundant as B. papayensis. We are of the opinion that, at this moment, B. papayensis and B. yothersi do not present risks to the production of coffee for the studied plantations. However, as the coffee-producing regions of Mexico are ecologically diverse, it will be important to continue examining the status of Brevipalpus mite populations in other regions in Mexico.


Assuntos
Coffea , Ácaros , Altitude , Animais , Café , México
9.
Antonie Van Leeuwenhoek ; 114(11): 1889-1898, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34480669

RESUMO

An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110T, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110T was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541T (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491T (99.1%), Streptomyces luteoverticillatus NBRC 3840T (99.1%), Streptomyces coerulescens NBRC 12758T (99.1%), and Streptomyces iranensis HM 35T (99.0%). Strain CA3R110T contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H6), and MK-9(H8) as major menaquinone, iso-C16:0, iso-C15:0, C16:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA-DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110T could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110T represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110T (= TBRC 11245T = NBRC 114296T).


Assuntos
Actinobacteria , Coffea , Streptomyces , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Fosfolipídeos , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Streptomyces/genética , Tailândia
10.
Molecules ; 26(15)2021 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-34361765

RESUMO

In this study, the aroma profile of 10 single origin Arabica coffees originating from eight different growing locations, from Central America to Indonesia, was analyzed using Headspace SPME-GC-MS as the analytical method. Their roasting was performed under temperature-time conditions, customized for each sample to reach specific sensory brew characteristics in an attempt to underline the customization of roast profiles and implementation of separate roastings followed by subsequent blending as a means to tailor cup quality. A total of 138 volatile compounds were identified in all coffee samples, mainly furan (~24-41%) and pyrazine (~25-39%) derivatives, many of which are recognized as coffee key odorants, while the main formation mechanism was the Maillard reaction. Volatile compounds' composition data were also chemometrically processed using the HCA Heatmap, PCA and HCA aiming to explore if they meet the expected aroma quality attributes and if they can be an indicator of coffee origin. The desired brew characteristics of the samples were satisfactorily captured from the volatile compounds formed, contributing to the aroma potential of each sample. Furthermore, the volatile compounds presented a strong variation with the applied roasting conditions, meaning lighter roasted samples were efficiently differentiated from darker roasted samples, while roasting degree exceeded the geographical origin of the coffee. The coffee samples were distinguished into two groups, with the first two PCs accounting for 73.66% of the total variation, attributed mainly to the presence of higher quantities of furans and pyrazines, as well as to other chemical classes (e.g., dihydrofuranone and phenol derivatives), while HCA confirmed the above results rendering roasting conditions as the underlying criterion for differentiation.


Assuntos
Coffea/química , Café/química , Furanos/química , Odorantes/análise , Pirazinas/química , Compostos Orgânicos Voláteis/química , América Central , Coffea/metabolismo , Café/metabolismo , Etiópia , Furanos/classificação , Furanos/isolamento & purificação , Furanos/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Temperatura Alta , Humanos , Indonésia , Reação de Maillard , Análise de Componente Principal , Pirazinas/classificação , Pirazinas/isolamento & purificação , Pirazinas/metabolismo , Sementes/química , Paladar/fisiologia , Compostos Orgânicos Voláteis/classificação , Compostos Orgânicos Voláteis/isolamento & purificação , Compostos Orgânicos Voláteis/metabolismo
11.
An Acad Bras Cienc ; 93(suppl 3): e20201649, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34378636

RESUMO

The objective of the present work was the molecular characterization of 11 parents and 101 hybrid progenies of conilon coffee, obtained through diallel crosses from the breeding program of the Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (Incaper, ES, Brazil). The analyses were performed with 18 Simple Sequence Repeat (SSR) molecular markers, obtaining a total of 32 alleles. SSR markers were classified as moderately informative (PIC = 0.37), being efficient in characterizing individuals. High genetic diversity was verified in the 112 genotypes, based on the greater values of observed heterozygosity about to the expected heterozygosity (0.55 and 0.44, respectively), negative values for the fixation index (F) (-0.14), and the formation of distinct groups by UPGMA. These results indicate high genetic variability among the conilon coffee genitors, which remained similar and persisting in the progenies. The average dissimilarity between parents was 0.29 and between progenies 0.34. The progenies 38 and 40 and the parent P11 were considered the most divergent in the study. The genetic variability found can be explored in the genetic breeding of the conilon coffee and guide crossings between diversified and compatible genetic materials, for the composition of novel cultivars for the state of Espírito Santo.


Assuntos
Coffea/genética , Variação Genética , Genótipo , Hibridização Genética , Repetições de Microssatélites , Melhoramento Vegetal
12.
Food Res Int ; 147: 110544, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34399521

RESUMO

This work aimed to study the composition differences of roasted beans between 12 coffee cultivars (Catimor 7963, HIBRIDO DE TIMOR, Ruiru 11, Castillo, DTARI 296, DTARI 366, DTARI 392, DTARI 585, SL28, SL34, Catuai-Amarelo and Catuai-Vermelho) from Bourbon-Typica group and Introgressed group under subtropical humid monsoon climate. The water-soluble compounds of roasted coffee beans were characterized by proton nuclear magnetic resonance spectroscopy (1H NMR), and the aroma components were analyzed by static headspace gas chromatography mass spectrometry (SHS-GC/MS). In total, 20 water soluble compounds and 43 volatile compounds were identified. Both water-soluble and volatile compounds are rich in acidic substances, and the content varied depending on the cultivars. Furthermore, principal component analysis (PCA) clustered 12 coffee cultivars into four groups. The four different chemically defined clusters of Arabica cultivars produced by chemical differences cannot reflect the traditional grouping based on introgressed, and it is one-sided to judge coffee quality based on lineage. These results give further insight into the quality characteristics of different coffee cultivars, which is of great significance for guiding the adjustment of cultivars' structure and the breeding of new cultivars.


Assuntos
Coffea , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Melhoramento Vegetal , Espectroscopia de Prótons por Ressonância Magnética , Sementes
13.
Molecules ; 26(14)2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34299444

RESUMO

We aimed to analyze the chemical compositions in Arabica coffee bean extracts, assess the relevant antioxidant and iron-chelating activities in coffee extracts and instant coffee, and evaluate the toxicity in roasted coffee. Coffee beans were extracted using boiling, drip-filtered and espresso brewing methods. Certain phenolics were investigated including trigonelline, caffeic acid and their derivatives, gallic acid, epicatechin, chlorogenic acid (CGA) and their derivatives, p-coumaroylquinic acid, p-coumaroyl glucoside, the rutin and syringic acid that exist in green and roasted coffee extracts, along with dimethoxycinnamic acid, caffeoylarbutin and cymaroside that may be present in green coffee bean extracts. Different phytochemicals were also detected in all of the coffee extracts. Roasted coffee extracts and instant coffees exhibited free-radical scavenging properties in a dose-dependent manner, for which drip coffee was observed to be the most effective (p < 0.05). All coffee extracts, instant coffee varieties and CGA could effectively bind ferric ion in a concentration-dependent manner resulting in an iron-bound complex. Roasted coffee extracts were neither toxic to normal mononuclear cells nor breast cancer cells. The findings indicate that phenolics, particularly CGA, could effectively contribute to the iron-chelating and free-radical scavenging properties observed in coffee brews. Thus, coffee may possess high pharmacological value and could be utilized as a health beverage.


Assuntos
Coffea/química , Sequestradores de Radicais Livres/análise , Proteínas de Ligação ao Ferro/análise , Alcaloides , Antioxidantes/farmacologia , Linhagem Celular Tumoral , Ácido Clorogênico/farmacologia , Cromatografia Líquida de Alta Pressão/métodos , Coffea/toxicidade , Café/química , Café/toxicidade , Temperatura Alta , Humanos , Ferro/análise , Espectrometria de Massas/métodos , Fenóis/farmacologia , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/química , Extratos Vegetais/análise , Extratos Vegetais/química , Sementes/química
14.
Molecules ; 26(14)2021 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-34299581

RESUMO

Coffee cherry is a rich source of chlorogenic acids (CGAs) and caffeine. In this study we examined the potential antioxidant activity and enzyme inhibitory effects of whole coffee cherries (WCC) and their two extracts on α-amylase, α-glucosidase and acetylcholinesterase (AChE) activities, which are targets for the control of diabetes and Alzheimer's diseases. Whole coffee cherry extract 40% (WCCE1) is rich in chlorogenic acid compounds, consisting of a minimum of 40% major isomers, namely 3-caffeoylquinic acids, 4-caffeoylquinic acids, 5-caffeoylquinic acids, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 4-feruloylquinc acid, and 5-feruloylquinc acid. Whole coffee cherry extract 70% (WCCE2) is rich in caffeine, with a minimum of 70%. WCCE1 inhibited the activities of digestive enzymes α-amylase and α-glucosidase, and WCCE2 inhibited acetylcholinesterase activities with their IC50 values of 1.74, 2.42, and 0.09 mg/mL, respectively. Multiple antioxidant assays-including DPPH, ABTS, FRAP, ORAC, HORAC, NORAC, and SORAC-demonstrated that WCCE1 has strong antioxidant activity.


Assuntos
Antioxidantes/química , Inibidores da Colinesterase/química , Coffea/química , Frutas/química , Inibidores de Glicosídeo Hidrolases/química , Compostos Fitoquímicos/química , Extratos Vegetais/química , Humanos
15.
Molecules ; 26(11)2021 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-34199752

RESUMO

Coffee has been studied for its health benefits, including prevention of several chronic diseases, such as type 2 diabetes mellitus, cancer, Parkinson's, and liver diseases. Chlorogenic acid (CGA), an important component in coffee beans, was shown to possess antiviral activity against viruses. However, the presence of caffeine in coffee beans may also cause insomnia and stomach irritation, and increase heart rate and respiration rate. These unwanted effects may be reduced by decaffeination of green bean Arabica coffee (GBAC) by treatment with dichloromethane, followed by solid-phase extraction using methanol. In this study, the caffeine and chlorogenic acid (CGA) level in the coffee bean from three different areas in West Java, before and after decaffeination, was determined and validated using HPLC. The results showed that the levels of caffeine were reduced significantly, with an order as follows: Tasikmalaya (2.28% to 0.097% (97 ppm), Pangalengan (1.57% to 0.049% (495 ppm), and Garut (1.45% to 0.00002% (0.2 ppm). The CGA levels in the GBAC were also reduced as follows: Tasikmalaya (0.54% to 0.001% (118 ppm), Pangalengan (0.97% to 0.0047% (388 ppm)), and Garut (0.81% to 0.029% (282 ppm). The decaffeinated samples were then subjected to the H5N1 neuraminidase (NA) binding assay to determine its bioactivity as an anti-influenza agent. The results show that samples from Tasikmalaya, Pangalengan, and Garut possess NA inhibitory activity with IC50 of 69.70, 75.23, and 55.74 µg/mL, respectively. The low level of caffeine with a higher level of CGA correlates with their higher levels of NA inhibitory, as shown in the Garut samples. Therefore, the level of caffeine and CGA influenced the level of NA inhibitory activity. This is supported by the validation of CGA-NA binding interaction via molecular docking and pharmacophore modeling; hence, CGA could potentially serve as a bioactive compound for neuraminidase activity in GBAC.


Assuntos
Cafeína/análise , Ácido Clorogênico/análise , Coffea/química , Virus da Influenza A Subtipo H5N1/enzimologia , Cloreto de Metileno/farmacologia , Neuraminidase/antagonistas & inibidores , Cafeína/efeitos adversos , Cafeína/farmacologia , Ácido Clorogênico/química , Ácido Clorogênico/farmacologia , Cromatografia Líquida de Alta Pressão , Coffea/efeitos dos fármacos , Manipulação de Alimentos , Humanos , Virus da Influenza A Subtipo H5N1/química , Virus da Influenza A Subtipo H5N1/efeitos dos fármacos , Concentração Inibidora 50 , Modelos Moleculares , Simulação de Acoplamento Molecular , Ligação Proteica , Extração em Fase Sólida , Proteínas Virais/antagonistas & inibidores
16.
Food Chem ; 365: 130471, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34252622

RESUMO

Agtron method is widely used in the industry to determine roasting degrees in whole and ground coffee but it suffers from some inconveniences associated with unavailability of equipment, high cost, and lack of reproductive results. This study investigates the feasibility to determine roasting degrees in coffee beans and ground specialty coffees using near-infrared (NIR) spectroscopy combined with multivariate calibration based on partial least squares (PLS) regression. Representative data sets were considered to cover all Agtron roasting profiles for whole and ground coffees. Proper development of models with outlier evaluation and complete validation using parameters of merit such as accuracy, adjust, residual prediction deviation, linearity, analytical sensitivity, and limits of detection and quantification are presented to prove their performance. The results indicated that predictive chemometric models, for intact coffee beans and ground coffee, could be used in the coffee industry as an alternative to Agtron, thus digitalizing the roasting quality control.


Assuntos
Coffea , Café , Calibragem , Sementes , Espectroscopia de Luz Próxima ao Infravermelho
17.
Molecules ; 26(12)2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34208702

RESUMO

Coffee is one of the most often consumed beverages almost all over the world. The multiplicity of beans, as well as the methods and parameters used to brew, encourages the optimization of the brewing process. The study aimed to analyze the effect of roasting beans, the brewing technique, and its parameters (time and water temperature) on antioxidant activity (determined using several in vitro methods), total polyphenols, flavonoids, and caffeine content. The infusions of unroasted and roasted Arabica beans from Brazil, Colombia, India, Peru, and Rwanda were analyzed. In general, infusions prepared from roasted beans had higher antioxidant activity and the content of above-mentioned compounds. The hot brew method was used to obtain infusions with a higher antioxidant activity, while the cold brew with higher caffeine content. The phenolic compound content in infusions prepared using both techniques depended on the roasting process. Moreover, the bean's origin, roasting process, and brewing technique had a significant effect on the tested properties, in contrary to brewing time and water temperature (below and above 90 °C), which had less impact. The results confirm the importance of coffee brewing optimization.


Assuntos
Café/química , Antioxidantes/análise , Bebidas , Compostos de Bifenilo/análise , Brasil , Cafeína/análise , Coffea/química , Colômbia , Índia , Peru , Fenóis/análise , Extratos Vegetais/química , Polifenóis/análise , Sementes/química , Temperatura , Fatores de Tempo , Água/química
18.
Food Chem ; 364: 130452, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34186481

RESUMO

The growing demand for excellent-quality coffees allied with their symbolic aestheticization that add value to the products favor the adulteration practices and consequently economic losses. So, this work proposes the suitability of NIR spectroscopy and Digital Images (from CACHAS) coupled with one-class classification methods for the non-destructive authentication of Gourmet ground roasted coffees. For this, Gourmet coffees (n = 44) were discriminated from Traditional (n = 36) and Superior (n = 10) by directly analyzing their powder without any sample preparation. Then, OC-PLS and dd-SIMCA were used to construct the models. dd-SIMCA using offset correction for NIR and RGB histogram for CACHAS achieved the best results, correctly recognizing all the 90 samples in both the training and test sets. Therefore, the proposed methodologies can be useful for both the consumers and regulatory agencies because it confirms the elevated standards of excellence of Brazilian specialty coffees, preventing fraudulent labeling, besides following the Principles of Green Analytical Chemistry.


Assuntos
Coffea , Café , Espectroscopia de Luz Próxima ao Infravermelho , Brasil , Coffea/química , Café/química , Sementes
19.
Int J Food Microbiol ; 354: 109282, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34140187

RESUMO

Coffee fermentation involves the action of microorganisms, whose metabolism has a significant influence on the composition of the beans and, consequently, on the beverage's sensory characteristics. In this study, the microbial diversity during the wet fermentation of Coffea arabica L. in the Sierra Nevada of Santa Marta (SNSM) in Colombia was explored by high-throughput sequencing and the resulting cup quality through the standards of the Specialty Coffee Association. The taxonomic assignment of sequence reads showed a high microbial diversity comprised of 695 bacterial and 156 fungal genera. The microbial community was dominated by the Lactic Acid Bacteria (LAB) Leuconostoc, the yeast Kazachstania, and the Acetic Acid Bacteria (AAB) Acetobacter. Co-occurrence relationships suggested synergistic patterns between populations of LAB-AAB, yeasts-AAB, Leuconostoc-Prevotella, LAB-ABB-Selenomonas, and yeasts-fungi-nonLAB-nonAAB, which may result in the production of metabolites that positively impact the sensory attributes of coffee. The beverages produced were classified as specialty coffees, and their score was positively influenced by the fungal richness and the abundance of unclassified Lactobacillales, Pichia, and Pseudomonas. The findings show the richness and microbial diversity of the SNSM and serve as input for future research such as the analysis of microbial-derived metabolites and the establishment of starter cultures in coffee processing that guarantee the generation of high-quality beverages, the standardization of processes, the reduction of economic losses, and the production of value-added products that allow taking advantage of specialty coffee market.


Assuntos
Biodiversidade , Coffea , Fermentação , Microbiota , Sementes , Bactérias/classificação , Bactérias/metabolismo , Coffea/microbiologia , Colômbia , Fungos/classificação , Fungos/metabolismo , Microbiota/fisiologia , Sementes/metabolismo , Sementes/microbiologia
20.
Artigo em Inglês | MEDLINE | ID: mdl-34106825

RESUMO

An endophytic actinobacterium, designated strain CA1R205T, was isolated from the surface-sterilized root of Coffea arabica L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. The strain produced light yellowish brown to dark brownish black substrate mycelium and greyish white aerial mycelium. The spiral spore chains were produced directly on aerial mycelium. CA1R205T was found to have ll-diaminopimelic acid in the cell peptidoglycan, galactose, glucose, mannose and ribose as whole-cell reducing sugars, MK-10(H4), MK-9(H6), MK-10(H2), MK-9(H4), MK-10(H6) and MK-10(H8) as menaquinones and iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were detected in the cells. These characteristics were consistent the typical chemotaxonomic properties of members the genus Streptomyces. The taxonomic affiliation at the genus level of this strain could be confirmed using its 16S rRNA gene sequence data. CA1R205T showed the highest 16S rRNA gene sequence similarity value to Streptomyces rapamycinicus NRRL B-5491T (98.9 %), followed by Streptomyces iranensis HM 35T (98.8 %). Digital DNA-DNA hybridization and average nucleotide identity-by blast (ANIb) values between CA1R205T and S. rapamycinicus NRRL B-5491T were 27.2 and 81.5 %, respectively. The DNA G+C content of genomic DNA was 70.7 mol%. Due to the differences in physiological, biochemical and genotypic data, CA1R205T could be discriminated from its closest neighbour. Thus, CA1R205T should be recognized as representing a novel species of the genus Streptomyces, for which the name Streptomyces coffeae sp. nov. is proposed. The type strain is CA1R205T (=TBRC 11244T=NBRC 114295T).


Assuntos
Coffea/microbiologia , Endófitos/isolamento & purificação , Raízes de Plantas/microbiologia , Streptomyces/isolamento & purificação , Composição de Bases/genética , Sequência de Bases , DNA Bacteriano/genética , Endófitos/genética , Funções Verossimilhança , Filogenia , RNA Ribossômico 16S/genética , Streptomyces/classificação , Tailândia
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