Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 67.877
Filtrar
1.
Sci Rep ; 14(1): 15335, 2024 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-38961176

RESUMO

Anastomotic leakage (AL) is a potentially life-threatening complication following colorectal cancer (CRC) resection. In this study, we aimed to unravel longitudinal changes in microbial structure before, during, and after surgery and to determine if microbial alterations may be predictive for risk assessment between sufficient anastomotic healing (AS) and AL prior surgery. We analysed the microbiota of 134 colon mucosal biopsies with 16S rRNA V1-V2 gene sequencing. Samples were collected from three location sites before, during, and after surgery, and patients received antibiotics after the initial collection and during surgery. The microbial structure showed dynamic surgery-related changes at different time points. Overall bacterial diversity and the abundance of some genera such as Faecalibacterium or Alistipes decreased over time, while the genera Enterococcus and Escherichia_Shigella increased. The distribution of taxa between AS and AL revealed significant differences in the abundance of genera such as Prevotella, Faecalibacterium and Phocaeicola. In addition to Phocaeicola, Ruminococcus2 and Blautia showed significant differences in abundance between preoperative sample types. ROC analysis of the predictive value of these genera for AL revealed an AUC of 0.802 (p = 0.0013). In summary, microbial composition was associated with postoperative outcomes, and the abundance of certain genera may be predictive of postoperative complications.


Assuntos
Fístula Anastomótica , Microbioma Gastrointestinal , Humanos , Masculino , Feminino , Idoso , Fístula Anastomótica/etiologia , Fístula Anastomótica/microbiologia , Pessoa de Meia-Idade , Microbioma Gastrointestinal/genética , Neoplasias Colorretais/cirurgia , Neoplasias Colorretais/microbiologia , RNA Ribossômico 16S/genética , Cirurgia Colorretal/efeitos adversos , Mucosa Intestinal/microbiologia , Mucosa Intestinal/patologia , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Colo/microbiologia , Colo/cirurgia , Colo/patologia , Estudo de Prova de Conceito
2.
Pediatr Surg Int ; 40(1): 168, 2024 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-38954013

RESUMO

PURPOSE: This study describes the management of urinary incontinence (UI) in eight girls with congenital pouch colon (CPC) associated with anorectal malformation (ARM). METHODS: From 2013 to 2015, six girls with CPC and UI underwent bladder neck reconstruction (BNR). Four girls had complete UI (CUI) and two girls partial UI (PUI). From 2019 to 2023, four girls, including two with failed BNR, underwent bladder neck closure (BNC) and augmentation cystoplasty (AC) with a continent stoma. Subtypes of CPC were Complete CPC (n = 7) and Incomplete CPC (n = 1). All girls had a double vagina; short, wide urethra; and reduced bladder capacity with an open, incompetent bladder neck (BNI). During BNR, a neourethra was constructed from a 1.5-2 cm-wide and 1.5-3-cm-long trigonal strip. During BNC, AC was performed using a 20 cm ileal segment (n = 3) and by a colonic pouch segment, preserved during earlier colorraphy (n = 1). Continent stoma included a Monti's channel (n = 3) and appendicovesicostomy (n = 1). RESULTS: BNR produced moderate improvement of UI (n = 2), while UI was still very severe (n = 4). During BNC, intraoperative complications included iatrogenic vaginal tears (n = 4). Early complications included partial dehiscence of the ileocystoplasty (n = 1), partial adhesive small bowel obstruction (n = 1), and difficulty in stomal catheterization with prolonged drainage from the pelvic drain (n = 1). Late complications included unilateral grade II vesicoureteric reflux (n = 2) and vesicovaginal fistula (VVF) (n = 2) needing trans-vaginal closure in one girl. Urinary stones (n = 2) with stomal leakage of urine in one girl needed open cystolithotomy twice (n = 1), and endoscopic lithotripsy (n = 1). At follow-up, all patients have high overall satisfaction with the procedure and their continence status. CONCLUSIONS: BNC with AC and a catheterizable stoma satisfactorily achieves continence in girls with CPC and UI, vastly improving quality of life. If lower urinary tract (LUT) anatomy is favorable, BNR with/without AC can be the initial surgical procedure. BNC should be the primary procedure in girls with unfavorable LUT anatomy and for failed BNR. LEVEL OF EVIDENCE: IV.


Assuntos
Incontinência Urinária , Humanos , Feminino , Incontinência Urinária/cirurgia , Incontinência Urinária/etiologia , Malformações Anorretais/cirurgia , Malformações Anorretais/complicações , Criança , Colo/cirurgia , Colo/anormalidades , Pré-Escolar , Procedimentos de Cirurgia Plástica/métodos , Estudos Retrospectivos , Bexiga Urinária/cirurgia , Bexiga Urinária/anormalidades , Lactente
3.
Gut Microbes ; 16(1): 2361493, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38958039

RESUMO

The juxtaposition of well-oxygenated intestinal colonic tissue with an anerobic luminal environment supports a fundamentally important relationship that is altered in the setting of intestinal injury, a process likely to be relevant to diseases such as inflammatory bowel disease. Herein, using two-color phosphorometry to non-invasively quantify both intestinal tissue and luminal oxygenation in real time, we show that intestinal injury induced by DSS colitis reduces intestinal tissue oxygenation in a spatially defined manner and increases the flux of oxygen from the tissue into the gut lumen. By characterizing the composition of the microbiome in both DSS colitis-affected gut and in a bioreactor containing a stable human fecal community exposed to microaerobic conditions, we provide evidence that the increased flux of oxygen into the gut lumen augments glycan degrading bacterial taxa rich in glycoside hydrolases which are known to inhabit gut mucosal surface. Continued disruption of the intestinal mucus barrier through such a mechanism may play a role in the perpetuation of the intestinal inflammatory process.


Assuntos
Bactérias , Colite , Microbioma Gastrointestinal , Mucosa Intestinal , Oxigênio , Colite/microbiologia , Colite/induzido quimicamente , Colite/metabolismo , Animais , Humanos , Oxigênio/metabolismo , Bactérias/metabolismo , Bactérias/classificação , Bactérias/isolamento & purificação , Bactérias/genética , Camundongos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Fezes/microbiologia , Camundongos Endogâmicos C57BL , Sulfato de Dextrana , Colo/microbiologia , Colo/metabolismo , Masculino
4.
BMC Surg ; 24(1): 202, 2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38965517

RESUMO

BACKGROUND: The preservation of the left colic artery (LCA) has emerged as a preferred approach in laparoscopic radical resection for rectal cancer. However, preserving the LCA while simultaneously dissecting the NO.253 lymph node can create a mesenteric defect between the inferior mesenteric artery (IMA), the LCA, and the inferior mesenteric vein (IMV). This defect could act as a potential "hernia ring," increasing the risk of developing an internal hernia after surgery. The objective of this study was to introduce a novel technique designed to mitigate the risk of internal hernia by filling mesenteric defects with autologous tissue. METHODS: This new technique was performed on eighteen patients with rectal cancer between January 2022 and June 2022. First of all, dissected the lymphatic fatty tissue on the main trunk of IMA from its origin until the LCA and sigmoid artery (SA) or superior rectal artery (SRA) were exposed and then NO.253 lymph node was dissected between the IMA, LCA and IMV. Next, the SRA or SRA and IMV were sequentially ligated and cut off at an appropriate location away from the "hernia ring" to preserve the connective tissue between the "hernia ring" and retroperitoneum. Finally, after mobilization of distal sigmoid, on the lateral side of IMV, the descending colon was mobilized cephalad. Patients'preoperative baseline characteristics and intraoperative, postoperative complications were examined. RESULTS: All patients' potential "hernia rings" were closed successfully with our new technique. The median operative time was 195 min, and the median intraoperative blood loss was 55 ml (interquartile range 30-90). The total harvested lymph nodes was 13.0(range12-19). The median times to first flatus and liquid diet intake were both 3.0 days. The median number of postoperative hospital days was 8.0 days. One patient had an injury to marginal arterial arch, and after mobolization of splenic region, tension-free anastomosis was achieved. No other severe postoperative complications such as abdominal infection, anastomotic leakage, or bleeding were observed. CONCLUSIONS: This technique is both safe and effective for filling the mesenteric defect, potentially reducing the risk of internal hernia following laparoscopic NO.253 lymph node dissection and preservation of the left colic artery in rectal cancer surgeries.


Assuntos
Hérnia Interna , Laparoscopia , Excisão de Linfonodo , Complicações Pós-Operatórias , Neoplasias Retais , Humanos , Neoplasias Retais/cirurgia , Excisão de Linfonodo/métodos , Laparoscopia/métodos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Complicações Pós-Operatórias/prevenção & controle , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologia , Hérnia Interna/prevenção & controle , Hérnia Interna/etiologia , Artéria Mesentérica Inferior/cirurgia , Colo/cirurgia , Colo/irrigação sanguínea
5.
Tech Coloproctol ; 28(1): 76, 2024 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-38954099

RESUMO

BACKGROUND: Colorectal anastomotic leakage causes severe consequences for patients and healthcare system as it will lead to increased consumption of hospital resources and costs. Technological improvements in anastomotic devices could reduce the incidence of leakage and its economic impact. The aim of the present study was to assess if the use of a new powered circular stapler is cost-effective. METHOD: This observational study included patients undergoing left-sided circular stapled colorectal anastomosis between January 2018 and December 2021. Propensity score matching was carried out to create two comparable groups depending on whether the anastomosis was performed using a manual or powered circular device. The rate of anastomotic leakage, its severity, the consumption of hospital resources, and its cost were the main outcome measures. A cost-effectiveness analysis comparing the powered circular stapler versus manual circular staplers was performed. RESULTS: A total of 330 patients were included in the study, 165 in each group. Anastomotic leakage rates were significantly different (p = 0.012): 22 patients (13.3%) in the manual group versus 8 patients (4.8%) in the powered group. The effectiveness of the powered stapler and manual stapler was 98.27% and 93.69%, respectively. The average cost per patient in the powered group was €6238.38, compared with €9700.12 in the manual group. The incremental cost-effectiveness ratio was - €74,915.28 per patient without anastomotic complications. CONCLUSION: The incremental cost of powered circular stapler compared with manual devices was offset by the savings from lowered incidence and cost of management of anastomotic leaks.


Assuntos
Anastomose Cirúrgica , Fístula Anastomótica , Colo , Análise Custo-Benefício , Reto , Grampeadores Cirúrgicos , Grampeamento Cirúrgico , Humanos , Fístula Anastomótica/prevenção & controle , Fístula Anastomótica/economia , Fístula Anastomótica/etiologia , Feminino , Grampeadores Cirúrgicos/economia , Masculino , Anastomose Cirúrgica/efeitos adversos , Anastomose Cirúrgica/economia , Anastomose Cirúrgica/instrumentação , Anastomose Cirúrgica/métodos , Pessoa de Meia-Idade , Idoso , Incidência , Grampeamento Cirúrgico/economia , Grampeamento Cirúrgico/métodos , Grampeamento Cirúrgico/efeitos adversos , Grampeamento Cirúrgico/instrumentação , Colo/cirurgia , Reto/cirurgia , Pontuação de Propensão , Adulto , Análise de Custo-Efetividade
6.
Braz J Med Biol Res ; 57: e13306, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38958363

RESUMO

Arbutin is utilized in traditional remedies to cure numerous syndromes because of its anti-microbial, antioxidant, and anti-inflammatory properties. This study aimed to evaluate chemopreventive effects of arbutin on azoxymethane (AOM)-induced colon aberrant crypt foci (ACF) in rats. Five groups of rats were used: normal control group (rats injected hypodermically with sterile phosphate-buffered saline once per week for two weeks) and groups 2-5, which were subcutaneously inoculated with 15 mg/kg AOM once a week for two weeks. AOM control and 5-fluorouracil (5-FU) control groups were fed 10% Tween orally daily for 8 weeks using a feeding tube. The treated groups were fed 30 and 60 mg/kg arbutin every day for 2 months. ACF from the AOM control group had aberrant nuclei in addition to multilayered cells and an absence of goblet cells. The negative control group displayed spherical cells and nuclei in basal positions. Histological examination revealed a reduced number of AFC cells from colon tissues of the 5-FU reference group. Arbutin-fed animals showed down-regulation of proliferating cell nuclear antigen (PCNA) and up-regulation of Bax protein compared to AOM control. Rats fed with arbutin displayed a significant increase of superoxide dismutase (SOD) and catalase (CAT) activities in colon tissue homogenates compared to the AOM control group. In conclusion, arbutin showed therapeutic effects against colorectal cancer, explained by its ability to significantly decrease ACF, down-regulate PCNA protein, and up-regulate Bax protein. In addition, arbutin significantly increased SOD and CAT, and decreased malondialdehyde (MDA) levels, which might be due to its anti-proliferative and antioxidant properties.


Assuntos
Focos de Criptas Aberrantes , Arbutina , Azoximetano , Antígeno Nuclear de Célula em Proliferação , Proteína X Associada a bcl-2 , Animais , Focos de Criptas Aberrantes/induzido quimicamente , Focos de Criptas Aberrantes/patologia , Focos de Criptas Aberrantes/prevenção & controle , Focos de Criptas Aberrantes/tratamento farmacológico , Antígeno Nuclear de Célula em Proliferação/metabolismo , Masculino , Arbutina/farmacologia , Ratos , Proteína X Associada a bcl-2/metabolismo , Colo/efeitos dos fármacos , Colo/patologia , Ratos Wistar , Fluoruracila , Carcinógenos
7.
Tech Coloproctol ; 28(1): 82, 2024 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-38981897

RESUMO

BACKGROUND: Although functional end-to-end anastomosis (FEEA) using a stapler in the colorectal field has been recognised worldwide, the technique varies by surgeon, and the safety of anastomosis using different techniques is unknown. METHODS: This multicentre prospective observational cohort study was conducted by the KYCC Study Group in Yokohama, Japan, and included patients who underwent colonic resection at seven centres between April 2020 and March 2022. This study compared the incidence of surgery-related abdominal complications (SAC: anastomotic leakage [AL], anastomotic bleeding, intra-abdominal abscess, enteritis, ileus, surgical site infection, and other abdominal complications) between two different methods of FEEA (one-step [OS] method: simultaneous anastomosis and bowel resection; two-step [TS] method: anastomosis after bowel resection). Complications of Clavien-Dindo classification grade 2 or higher were assessed. RESULTS: Among 293 eligible cases, the OS and TS methods were used in 194 (66.2%) and 99 (33.8%) patients, respectively. The baseline characteristics were similar between the groups. The OS method used fewer staplers (three vs. four staplers, p < 0.00001). There were no significant differences in SAC rate between the OS (19.1%) and the TS (16.2%) groups (p = 0.44). The OS group had four cases (2.1%) of AL (two patients; grade 3, two patients; grade 2) while the TS group had one case (1.0%) of grade 2 AL (p = 0.67). Multivariate logistic regression analysis showed that male sex (odds ratio [OR] 3.95; p < 0.00001), an open surgical approach (OR 2.36; p = 0.03), and longer operative duration (OR,2.79; p = 0.002) were independent predictors of complications, whereas the OS method was not an independent predictor (OR 1.17; p = 0.66). CONCLUSIONS: The OS and the TS technique for stapled colonic anastomosis in a FEEA had a similar postoperative complication rate. TRIAL REGISTRATION NUMBER: UMIN000039902 (registration date 23 March 2020).


Assuntos
Anastomose Cirúrgica , Colectomia , Complicações Pós-Operatórias , Humanos , Masculino , Feminino , Anastomose Cirúrgica/métodos , Anastomose Cirúrgica/efeitos adversos , Estudos Prospectivos , Idoso , Japão , Pessoa de Meia-Idade , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologia , Colectomia/métodos , Colectomia/efeitos adversos , Colo/cirurgia , Fístula Anastomótica/etiologia , Fístula Anastomótica/epidemiologia , Incidência , Idoso de 80 Anos ou mais , Grampeamento Cirúrgico/métodos , População do Leste Asiático
8.
FASEB J ; 38(13): e23775, 2024 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-38967223

RESUMO

Inflammatory bowel disease (IBD) is a chronic disease of the gastrointestinal tract affecting millions of people. Here, we investigated the expression and functions of poly(ADP-ribose) polymerase 14 (Parp14), an important regulatory protein in immune cells, with an IBD patient cohort as well as two mouse colitis models, that is, IBD-mimicking oral dextran sulfate sodium (DSS) exposure and oral Salmonella infection. Parp14 was expressed in the human colon by cells in the lamina propria, but, in particular, by the epithelial cells with a granular staining pattern in the cytosol. The same expression pattern was evidenced in both mouse models. Parp14-deficiency caused increased rectal bleeding as well as stronger epithelial erosion, Goblet cell loss, and immune cell infiltration in DSS-exposed mice. The absence of Parp14 did not affect the mouse colon bacterial microbiota. Also, the colon leukocyte populations of Parp14-deficient mice were normal. In contrast, bulk tissue RNA-Seq demonstrated that the colon transcriptomes of Parp14-deficient mice were dominated by abnormalities in inflammation and infection responses both prior and after the DSS exposure. Overall, the data indicate that Parp14 has an important role in the maintenance of colon epithelial barrier integrity. The prognostic and predictive biomarker potential of Parp14 in IBD merits further investigation.


Assuntos
Colite , Sulfato de Dextrana , Camundongos Endogâmicos C57BL , Poli(ADP-Ribose) Polimerases , Animais , Feminino , Humanos , Masculino , Camundongos , Colite/genética , Colite/induzido quimicamente , Colite/patologia , Colo/patologia , Colo/metabolismo , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças , Microbioma Gastrointestinal , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/patologia , Doenças Inflamatórias Intestinais/metabolismo , Camundongos Knockout , Poli(ADP-Ribose) Polimerases/metabolismo , Poli(ADP-Ribose) Polimerases/genética , Poli(ADP-Ribose) Polimerases/deficiência
9.
Pediatr Surg Int ; 40(1): 185, 2024 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-38997605

RESUMO

PURPOSE: This study aimed to investigate the impact of hepatocyte growth factor (HGF) on colonic morphology and gut microbiota in a rat model of short bowel syndrome (SBS). METHODS: SD rats underwent jugular vein catheterization for total parenteral nutrition (TPN) and 90% small bowel resection [TPN + SBS (control group) or TPN + SBS + intravenous HGF (0.3 mg/kg/day, HGF group)]. Rats were harvested on day 7. Colonic morphology, gut microflora, tight junction, and Toll-like receptor-4 (TLR4) were evaluated. RESULTS: No significant differences were observed in the colonic morphological assessment. No significant differences were observed in the expression of tight junction-related genes in the proximal colon. However, the claudin-1 expression tended to increase and the claudin-3 expression tended to decrease in the distal colon of the HGF group. The Verrucomicrobiota in the gut microflora of the colon tended to increase in the HGF group. The abundance of most LPS-producing microbiota was lower in the HGF group than in the control group. The gene expression of TLR4 was significantly downregulated in the distal colon of the HGF group. CONCLUSION: HGF may enhance the mucus barrier through the tight junctions or gut microbiome in the distal colon.


Assuntos
Colo , Modelos Animais de Doenças , Microbioma Gastrointestinal , Fator de Crescimento de Hepatócito , Ratos Sprague-Dawley , Síndrome do Intestino Curto , Animais , Ratos , Fator de Crescimento de Hepatócito/metabolismo , Fator de Crescimento de Hepatócito/genética , Microbioma Gastrointestinal/efeitos dos fármacos , Colo/microbiologia , Colo/patologia , Síndrome do Intestino Curto/metabolismo , Síndrome do Intestino Curto/microbiologia , Masculino , Receptor 4 Toll-Like/metabolismo , Receptor 4 Toll-Like/genética , Junções Íntimas/efeitos dos fármacos , Junções Íntimas/metabolismo , Claudina-1/metabolismo , Claudina-1/genética
10.
Sci Rep ; 14(1): 15798, 2024 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-38982226

RESUMO

The present study aimed to explore the underlying mechanism of bile reflux-inducing chronic atrophic gastritis (CAG) with colonic mucosal lesion. The rat model of CAG with colonic mucosal lesion was induced by free-drinking 20 mmol/L sodium deoxycholate to simulate bile reflux and 2% cold sodium salicylate for 12 weeks. In comparison to the control group, the model rats had increased abundances of Bacteroidetes and Firmicutes but had decreased abundances of Proteobacteria and Fusobacterium. Several gut bacteria with bile acids transformation ability were enriched in the model group, such as Blautia, Phascolarctobacter, and Enterococcus. The cytotoxic deoxycholic acid and lithocholic acid were significantly increased in the model group. Transcriptome analysis of colonic tissues presented that the down-regulated genes enriched in T cell receptor signaling pathway, antigen processing and presentation, Th17 cell differentiation, Th1 and Th2 cell differentiation, and intestinal immune network for IgA production in the model group. These results suggest that bile reflux-inducing CAG with colonic mucosal lesion accompanied by gut dysbacteriosis, mucosal immunocompromise, and increased gene expressions related to repair of intestinal mucosal injury.


Assuntos
Colo , Ácido Desoxicólico , Gastrite Atrófica , Microbioma Gastrointestinal , Mucosa Intestinal , Animais , Gastrite Atrófica/microbiologia , Gastrite Atrófica/imunologia , Gastrite Atrófica/patologia , Gastrite Atrófica/induzido quimicamente , Ratos , Mucosa Intestinal/patologia , Mucosa Intestinal/imunologia , Mucosa Intestinal/microbiologia , Mucosa Intestinal/efeitos dos fármacos , Masculino , Colo/patologia , Colo/efeitos dos fármacos , Microbioma Gastrointestinal/efeitos dos fármacos , Modelos Animais de Doenças , Imunidade nas Mucosas/efeitos dos fármacos , Ratos Sprague-Dawley , Doença Crônica
11.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 46(3): 334-340, 2024 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-38953257

RESUMO

Objective To explore the relationship between the expression levels of microRNA-155 (miR-155) and suppressor of cytokine signaling 1 (SOCS1) in the colonic mucosal tissue of patients with ulcerative colitis (UC) and the severity of the disease.Methods A total of 130 UC patients admitted to the Second Affiliated Hospital of Hebei North University from September 2021 to June 2023 were selected.According to the modified Mayo score system,the patients were assigned into an active stage group (n=85) and a remission stage group (n=45).According to the modified Truelove and Witts classification criteria,the UC patients at the active stage were assigned into a mild group (n=35),a moderate group (n=30),and a severe group (n=20).A total of 90 healthy individuals who underwent colonoscopy for physical examination or those who had normal colonoscopy results after single polypectomy and excluded other diseases were selected as the control group.The colonic mucosal tissues of UC patients with obvious lesions and the colonic mucosal tissue 20 cm away from the anus of the control group were collected.The levels of miR-155 and SOCS1 mRNA in tissues were determined by fluorescence quantitative PCR,and the expression of SOCS1 protein in tissues was determined by immunohistochemistry.The correlations of the levels of miR-155 and SOCS1 mRNA in the colonic mucosal tissue with the modified Mayo score of UC patients were analyzed.The values of the levels of miR-155 and SOCS1 mRNA in predicting the occurrence of severe illness in the UC patients at the active stage were evaluated.Results Compared with the control group and the remission stage group,the active stage group showed up-regulated expression level of miR-155,down-regulated level of SOCS1 mRNA,and decreased positive rate of SOCS1 protein in the colonic mucosal tissue (all P<0.001).The expression level of miR-155 and modified Mayo score in colonic mucosal tissues of UC patients at the active stage increased,while the mRNA level of SOCS1 was down-regulated as the disease evolved from being mild to severe (all P<0.001).The modified Mayo score was positively correlated with the miR-155 level and negative correlated with the mRNA level of SOCS1 in colonic mucosal tissues of UC patients (all P<0.001).The high miR-155 level (OR=2.762,95%CI=1.284-5.944,P=0.009),low mRNA level of SOCS1 (OR=2.617,95%CI=1.302-5.258,P=0.007),and modified Mayo score≥12 points (OR=3.232,95%CI=1.450-7.204,P=0.004) were all risk factors for severe disease in the UC patients at the active stage.The area under curve of miR-155 combined with SOCS1 mRNA in predicting severe illness in the UC patients at the active stage was 0.920.Conclusions The expression levels of miR-155 and SOCS1 mRNA were correlated with the disease severity in the UC patients at the active stage.The combination of the two indicators demonstrates good performance in predicting the occurrence of severe illness in UC patients at the active stage.


Assuntos
Colite Ulcerativa , Mucosa Intestinal , MicroRNAs , Índice de Gravidade de Doença , Proteína 1 Supressora da Sinalização de Citocina , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Colite Ulcerativa/metabolismo , Colite Ulcerativa/genética , Colite Ulcerativa/patologia , Proteína 1 Supressora da Sinalização de Citocina/genética , Proteína 1 Supressora da Sinalização de Citocina/metabolismo , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Colo/metabolismo , Colo/patologia , Feminino , Masculino , Pessoa de Meia-Idade , Adulto
13.
Pharmacol Res Perspect ; 12(4): e1234, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38961539

RESUMO

The association of hormonal contraception with increased risk of inflammatory bowel disease (IBD) observed in females suggests involvement of ovarian hormones, such as estradiol, and the estrogen receptors in the progression of intestinal inflammation. Here, we investigated the effects of prophylactic SERM2 and estradiol supplementation in dextran sulfate sodium-induced colitis using mice with intact ovaries and ovariectomized (OVX) female mice. We found that graded colitis score was threefold reduced in the OVX mice, compared to mice with intact ovaries. Estradiol supplementation, however, aggravated the colitis in OVX mice, increasing the colitis score to a similar level than what was observed in the intact mice. Further, we observed that immune infiltration and gene expression of inflammatory interleukins Il1b, Il6, and Il17a were up to 200-fold increased in estradiol supplemented OVX colitis mice, while a mild but consistent decrease was observed by SERM2 treatment in intact animals. Additionally, cyclo-oxygenase 2 induction was increased in the colon of colitis mice, in correlation with increased serum estradiol levels. Measured antagonist properties of SERM2, together with the other results presented here, indicates an exaggerating role of ERα signaling in colitis. Our results contribute to the knowledge of ovarian hormone effects in colitis and encourage further research on the potential use of ER antagonists in the colon, in order to alleviate inflammation.


Assuntos
Colite , Sulfato de Dextrana , Estradiol , Receptor alfa de Estrogênio , Ovariectomia , Animais , Feminino , Receptor alfa de Estrogênio/metabolismo , Colite/induzido quimicamente , Colite/metabolismo , Colite/tratamento farmacológico , Camundongos , Estradiol/farmacologia , Estradiol/sangue , Camundongos Endogâmicos C57BL , Estrogênios/farmacologia , Ciclo-Oxigenase 2/metabolismo , Modelos Animais de Doenças , Interleucina-17/metabolismo , Colo/patologia , Colo/efeitos dos fármacos , Colo/metabolismo , Interleucina-6/metabolismo , Interleucina-1beta/metabolismo
14.
Stress ; 27(1): 2374768, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38975691

RESUMO

Stress has been linked to the development of irritable bowel syndrome (IBS), and various methods have been explored to model IBS in combination with other stimuli. However, it remains unclear whether stress alone can induce IBS in animals. This study aimed to investigate the impact of chronic unpredictable mild stress (CUMS) on gastrointestinal sensation and function in mice and assess the potential of CUMS as a modeling approach for IBS. To evaluate the mice's behavior, we conducted open field test, sucrose preference test and weighed the mice, revealing that CUMS indeed induced anxiety and depression in the mice and caused weight loss. Further analyses, including fecal analysis, a total gastrointestinal transport test, and a colon propulsion test, demonstrated that CUMS led to abnormal defecation and disruptions in gastrointestinal motility in the mice. Additionally, the abdominal withdrawal reflex test indicated an increase in visceral sensitivity in CUMS-exposed mice. Histological examination using hematoxylin and eosin staining revealed no significant histological alterations in the colons of CUMS-exposed mice, but it did show a minor degree of inflammatory cell infiltration. In summary, the findings suggest that CUMS can replicate IBS-like symptoms in mice, offering a novel top-down approach to modeling IBS.


Assuntos
Modelos Animais de Doenças , Motilidade Gastrointestinal , Síndrome do Intestino Irritável , Estresse Psicológico , Animais , Estresse Psicológico/fisiopatologia , Estresse Psicológico/complicações , Masculino , Camundongos , Síndrome do Intestino Irritável/fisiopatologia , Motilidade Gastrointestinal/fisiologia , Ansiedade/fisiopatologia , Depressão/fisiopatologia , Camundongos Endogâmicos C57BL , Comportamento Animal , Defecação , Colo/fisiopatologia , Colo/patologia
15.
Sci Rep ; 14(1): 15706, 2024 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-38977770

RESUMO

Maintaining the mucus layer is crucial for the innate immune system. Urolithin A (Uro A) is a gut microbiota-derived metabolite; however, its effect on mucin production as a physical barrier remains unclear. This study aimed to elucidate the protective effects of Uro A on mucin production in the colon. In vivo experiments employing wild-type mice, NF-E2-related factor 2 (Nrf2)-deficient mice, and wild-type mice treated with an aryl hydrocarbon receptor (AhR) antagonist were conducted to investigate the physiological role of Uro A. Additionally, in vitro assays using mucin-producing cells (LS174T) were conducted to assess mucus production following Uro A treatment. We found that Uro A thickened murine colonic mucus via enhanced mucin 2 expression facilitated by Nrf2 and AhR signaling without altering tight junctions. Uro A reduced mucosal permeability in fluorescein isothiocyanate-dextran experiments and alleviated dextran sulfate sodium-induced colitis. Uro A treatment increased short-chain fatty acid-producing bacteria and propionic acid concentration. LS174T cell studies confirmed that Uro A promotes mucus production through the AhR and Nrf2 pathways. In conclusion, the enhanced intestinal mucus secretion induced by Uro A is mediated through the actions of Nrf-2 and AhR, which help maintain intestinal barrier function.


Assuntos
Colite , Cumarínicos , Mucosa Intestinal , Fator 2 Relacionado a NF-E2 , Receptores de Hidrocarboneto Arílico , Animais , Fator 2 Relacionado a NF-E2/metabolismo , Receptores de Hidrocarboneto Arílico/metabolismo , Camundongos , Mucosa Intestinal/metabolismo , Cumarínicos/farmacologia , Colite/metabolismo , Colite/induzido quimicamente , Mucina-2/metabolismo , Mucina-2/genética , Humanos , Colo/metabolismo , Camundongos Endogâmicos C57BL , Transdução de Sinais/efeitos dos fármacos , Masculino , Microbioma Gastrointestinal , Camundongos Knockout , Sulfato de Dextrana , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Função da Barreira Intestinal
16.
Molecules ; 29(13)2024 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-38999160

RESUMO

Chemically modified mandua starch was successfully synthesized and applied to coat mesalamine-loaded matrix tablets. The coating material was an aqueous dispersion of mandua starch modified by sodium trimetaphosphate and sodium tripolyphosphate. To investigate the colon-targeting release competence, chemically modified mandua starch film-coated mesalamine tablets were produced using the wet granulation method followed by dip coating. The effect of the coating on the colon-targeted release of the resultant delivery system was inspected in healthy human volunteers and rabbits using roentgenography. The results show that drug release was controlled when the coating level was 10% w/w. The release percentage in the upper gastric phase (pH 1.2, simulated gastric fluid) was less than 6% and reached up to 59.51% w/w after 14 h in simulated colonic fluid. In addition to in vivo roentgenographic studies in healthy rabbits, human volunteer studies proved the colon targeting efficiency of the formulation. These results clearly demonstrated that chemically modified mandua starch has high effectiveness as a novel aqueous coating material for controlled release or colon targeting.


Assuntos
Liberação Controlada de Fármacos , Mesalamina , Amido , Comprimidos , Mesalamina/química , Mesalamina/farmacocinética , Coelhos , Amido/química , Animais , Humanos , Concentração de Íons de Hidrogênio , Fosforilação , Preparações de Ação Retardada/química , Colo/metabolismo
17.
Nutrients ; 16(13)2024 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-38999794

RESUMO

Enterohemorrhagic Escherichia coli (EHEC) is a major food-borne pathogen that causes human disease ranging from diarrhea to life-threatening complications. Accumulating evidence demonstrates that the Western diet enhances the susceptibility to enteric infection in mice, but the effect of diet on EHEC colonization and the role of human gut microbiota remains unknown. Our research aimed to investigate the effects of a Standard versus a Western diet on EHEC colonization in the human in vitro Mucosal ARtificial COLon (M-ARCOL) and the associated changes in the gut microbiota composition and activities. After donor selection using simplified fecal batch experiments, two M-ARCOL bioreactors were inoculated with a human fecal sample (n = 4) and were run in parallel, one receiving a Standard diet, the other a Western diet and infected with EHEC O157:H7 strain EDL933. EHEC colonization was dependent on the donor and diet in the luminal samples, but was maintained in the mucosal compartment without elimination, suggesting a favorable niche for the pathogen, and may act as a reservoir. The Western diet also impacted the bacterial short-chain fatty acid and bile acid profiles, with a possible link between high butyrate concentrations and prolonged EHEC colonization. The work demonstrates the application of a complex in vitro model to provide insights into diet, microbiota, and pathogen interactions in the human gut.


Assuntos
Colo , Dieta Ocidental , Escherichia coli Êntero-Hemorrágica , Fezes , Microbioma Gastrointestinal , Humanos , Microbioma Gastrointestinal/fisiologia , Dieta Ocidental/efeitos adversos , Colo/microbiologia , Fezes/microbiologia , Infecções por Escherichia coli/microbiologia , Mucosa Intestinal/microbiologia , Mucosa Intestinal/metabolismo , Ácidos Graxos Voláteis/metabolismo , Ácidos e Sais Biliares/metabolismo , Escherichia coli O157
18.
Int J Mol Sci ; 25(13)2024 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-39000356

RESUMO

The glucose-lowering drug metformin alters the composition of the gut microbiome in patients with type 2 diabetes mellitus (T2DM) and other diseases. Nevertheless, most studies on the effects of this drug have relied on fecal samples, which provide limited insights into its local effects on different regions of the gut. Using a high-fat diet (HFD)-induced mouse model of T2DM, we characterize the spatial variability of the gut microbiome and associated metabolome in response to metformin treatment. Four parts of the gut as well as the feces were analyzed using full-length sequencing of 16S rRNA genes and targeted metabolomic analyses, thus providing insights into the composition of the microbiome and associated metabolome. We found significant differences in the gut microbiome and metabolome in each gut region, with the most pronounced effects on the microbiomes of the cecum, colon, and feces, with a significant increase in a variety of species belonging to Akkermansiaceae, Lactobacillaceae, Tannerellaceae, and Erysipelotrichaceae. Metabolomics analysis showed that metformin had the most pronounced effect on microbiome-derived metabolites in the cecum and colon, with several metabolites, such as carbohydrates, fatty acids, and benzenoids, having elevated levels in the colon; however, most of the metabolites were reduced in the cecum. Thus, a wide range of beneficial metabolites derived from the microbiome after metformin treatment were produced mainly in the colon. Our study highlights the importance of considering gut regions when understanding the effects of metformin on the gut microbiome and metabolome.


Assuntos
Diabetes Mellitus Tipo 2 , Dieta Hiperlipídica , Modelos Animais de Doenças , Microbioma Gastrointestinal , Metaboloma , Metformina , Metformina/farmacologia , Animais , Microbioma Gastrointestinal/efeitos dos fármacos , Dieta Hiperlipídica/efeitos adversos , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/microbiologia , Diabetes Mellitus Tipo 2/tratamento farmacológico , Camundongos , Metaboloma/efeitos dos fármacos , Masculino , Fezes/microbiologia , RNA Ribossômico 16S/genética , Hipoglicemiantes/farmacologia , Camundongos Endogâmicos C57BL , Ceco/microbiologia , Ceco/metabolismo , Ceco/efeitos dos fármacos , Colo/metabolismo , Colo/efeitos dos fármacos , Colo/microbiologia , Metabolômica/métodos
19.
Bull Exp Biol Med ; 177(1): 162-168, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38960963

RESUMO

In adult male C57BL/6 mice with high (HR) and low (LR) resistance to hypoxia, morphological features of colon tumors and blood parameters were evaluated 70 days after intraperitoneal injection of azoxymethane and subsequent consumption of 3 cycles of dextran sulfate sodium. On macroscopic analysis, tumors were found in the distal colon in 35% (7 of 20 animals) of HR and 31% (4 of 13 animals) of LR animals. Microscopic analysis of the distal colon revealed tumors in 75% (15 of 20 animals) of HR and 69% (9 of 13 animals) of LR mice. The tumors were presented by areas of glandular intraepithelial neoplasia and adenocarcinomas; the incidence and the area of the tumors did not differ in groups of HR and LR mice. The number of neuroendocrine and goblet cells in the distal colon mucosa in the areas of tumors was similar in the compared groups. However, in both HR and LR mice of the experimental groups, the content of goblet cells in tumors was lower and the content of endocrine cells was higher than in the corresponding control groups. In the peripheral blood, the erythrocyte count and hemoglobin content decreased in HR and LR mice of the experimental groups; the relative number of monocytes increased only in HR mice and the absolute number of lymphocytes and monocytes decreased in LR mice. Thus, 70 days after azoxymethane administration and dextran sulfate sodium consumption, the tumors in mice were presented by glandular intraepithelial neoplasia and adenocarcinomas, and their incidence and area did not differ between animals with different tolerance to hypoxia.


Assuntos
Adenocarcinoma , Azoximetano , Neoplasias do Colo , Sulfato de Dextrana , Camundongos Endogâmicos C57BL , Animais , Camundongos , Neoplasias do Colo/patologia , Neoplasias do Colo/induzido quimicamente , Neoplasias do Colo/metabolismo , Masculino , Sulfato de Dextrana/toxicidade , Azoximetano/toxicidade , Adenocarcinoma/patologia , Adenocarcinoma/induzido quimicamente , Adenocarcinoma/metabolismo , Hipóxia/patologia , Colo/patologia , Células Caliciformes/patologia , Células Caliciformes/metabolismo , Mucosa Intestinal/patologia , Hemoglobinas/metabolismo , Monócitos/patologia , Monócitos/metabolismo , Contagem de Eritrócitos
20.
Langenbecks Arch Surg ; 409(1): 214, 2024 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-39002002

RESUMO

PURPOSE: Ensuring optimal colonic perfusion is a critical step in every colorectal anastomosis. The aim of this study is to describe the concept of epiploic steal. METHODS: A literature review was performed to identify studies evaluating anastomotic blood supply. The fundamental principle of epiploic steal is outlined. RESULTS: Epiploic steal has not been previously evaluated in the literature, and likely has a negative effect on colonic blood supply. Resection of colonic epiploicae may improve perfusion at the distal most lengths of a mobilised colonic conduit where the anastomosis requires it. CONCLUSION: This novel concept has the potential to change practice and reduce colorectal anastomotic leak rates. Further clinical studies are required.


Assuntos
Anastomose Cirúrgica , Fístula Anastomótica , Colo , Humanos , Fístula Anastomótica/prevenção & controle , Colo/cirurgia , Colo/irrigação sanguínea , Reto/cirurgia , Reto/irrigação sanguínea , Colectomia/efeitos adversos , Colectomia/métodos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...