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1.
Mutat Res ; 850-851: 503161, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32247561

RESUMO

Through diet, people are chronically exposed to low doses of a large number of contaminants that could exhibit adverse health effects. Toxicological evaluation of food contaminants increases in complexity when the exposure involves chemical mixtures. The aim of this study is to investigate the genotoxic potential, through measuring ©H2AX induction, of six common mixtures of food contaminants to which French adult consumers are chronically exposed. Mixtures were identified by combining information from consumption surveys and contaminant concentration levels in foods. Both single and repeated exposures were evaluated in human liver-derived HepaRG cells. Our results indicated that after a single 24-h exposure, only one mixture induced genotoxicity, and that response occurred at the highest concentration tested. In contrast, we observed after repeated exposures over 3 or 7 days, induction of ©H2AX for all mixtures except one, and a time- and concentration-dependent manner toxicity for four mixtures. Interestingly, we also observed a non-monotonic cytotoxicity concentration-response for one mixture, which might reflect cellular adaptation to the exposure. In conclusion, our study demonstrated that longer-term treatments for in vitro toxicological evaluation, instead of the classical 24 h treatment, may be more relevant regarding human toxicology assessment.


Assuntos
Dano ao DNA/efeitos dos fármacos , Dieta/efeitos adversos , Contaminação de Alimentos/análise , Histonas/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Hepatócitos/efeitos dos fármacos , Humanos , Fígado/efeitos dos fármacos , Testes de Mutagenicidade
2.
Chin J Nat Med ; 18(3): 219-225, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32245592

RESUMO

The rapid detection of pathogenic bacteria is vital for the prevention of outbreaks of infectious diseases, including infections by the common foodborne bacteria E.coli and Salmonella Carbohydrate microarrays have been developed as a powerful method to investigate carbohydrate-protein interaction with only very small amounts of glycans, which show great potential for detect the carbohydrate mediated interaction with pathogens. Here, different mannose-coated microarrays were constructed and tested with E.coli (K-12 and BL-21) and Salmonella enterica strains (ATCC9184 and ATCC31685) exhibiting different mannose binding affinities. The optimized carbohydrate microarray was then applied to test the binding of 12 Salmonella enterica and 9 E.coli isolates from local patients for the first time and showed strong binding with certain serovars or subtypes. The results showed that microarray probed with the single mannose structure is not enough for the detection of bacteria with various serovars or subtypes, which contain a high degree of allelic variation in adhesin. We suggest that a complex carbohydrate microarray containing different glycan conformation may be needed for detection of different bacteria isolates.


Assuntos
Carboidratos/química , Escherichia coli/isolamento & purificação , Análise em Microsséries/métodos , Salmonella enterica/isolamento & purificação , Adesinas Bacterianas/química , Contaminação de Alimentos , Microbiologia de Alimentos , Humanos , Manose/química , Polissacarídeos/química
3.
Toxicon ; 180: 49-61, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32268155

RESUMO

Mycotoxins-contaminated milk could threaten human health; therefore, it is necessary to demonstrate the toxicological effect of mycotoxins in milk. Most recently, researchers have paid more attention to the immunotoxic effects of the individual cereal-contaminating mycotoxins, namely, zearalenone and deoxynivalenol. However, there is scant information about the intestinal immunotoxicity of aflatoxin M1 (AFM1), let alone that of a combination of AFM1 and ochratoxin A (OTA), which often co-occur in milk. To reveal the inflammatory response caused by these mycotoxins, expression of inflammation-related genes in differentiated Caco-2 cells was analyzed, demonstrating a synergistic effect of the mixture of AFM1 (4 µg/mL) and OTA (4 µg/mL). Integrative transcriptomic and proteomic analyses were also performed. A cross-omics analysis identified several mechanisms underlying this synergy: (i) compared with stimulation with either compound alone, combined use resulted in stronger induction of proteins involved in immunity-related pathways; (ii) combination of the two agents targeted different points in the same pathways; and (iii) combination of the two agents activated specific inflammation-related pathways. These results suggested that combined use of AFM1 and OTA might exacerbate intestinal inflammation, indicating that regulatory authorities should pay more attention to food contamination by multiple mycotoxins when performing risk assessments.


Assuntos
Aflatoxina M1/metabolismo , Imunotoxinas/metabolismo , Intestinos/efeitos dos fármacos , Ocratoxinas/metabolismo , Proteoma/metabolismo , Aflatoxina M1/genética , Animais , Células CACO-2 , Diferenciação Celular , Contaminação de Alimentos , Perfilação da Expressão Gênica , Humanos , Imunotoxinas/genética , Leite , Micotoxinas , Proteômica , Transcriptoma , Zearalenona
4.
Food Chem ; 317: 126448, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32114274

RESUMO

The chemometric issues related to the application of non-targeted analysis for the detection of food frauds were analyzed employing discriminant analysis and a one-class classifier. The similarities and differences between the two methods were investigated. The results of classification are characterized by a set of indices called figures of merit. They comprehensively characterized the quality and reliability of classification. The principle is illustrated using an actual example of Oregano herbs adulteration. The informative region 9000-4000 cm-1 of near-Infrared spectroscopy is used as analytical means. The results of the application of each method for Oregano data collection are presented. It is shown that the discriminant method is only partially appropriate for solving the authentication problem. One class classifier is a powerful and devoted for non-targeted analysis. The step by step analysis introduced in the paper can also be successfully utilized in apply for revealing of forgeries of various food products.


Assuntos
Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Fraude , Origanum/química , Análise Discriminante , Análise de Alimentos/estatística & dados numéricos , Contaminação de Alimentos/estatística & dados numéricos , Análise dos Mínimos Quadrados , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectroscopia de Luz Próxima ao Infravermelho/métodos
5.
Int J Food Microbiol ; 322: 108564, 2020 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-32163798

RESUMO

Campylobacter is one of the most important causative agents of foodborne illnesses worldwide. The poultry reservoir is the main source of Campylobacter. Within the broiler production chain, campylobacters can only multiply in the chicken's intestinal tract. Intervention at farm level to reduce Campylobacter is thus preferred, but despite extensive study, no highly effective solutions have been found to combat Campylobacter at farm level. Slaughterhouses are experiencing great pressure to deliver carcasses with low Campylobacter contamination even when they receive and slaughter Campylobacter colonized flocks. Since 2018, a process hygiene criterion (EU 2017/1495) with the critical limit of <1000 cfu/g neck skin has been implemented in EU Member States based on the calculation done at the time of the study that human campylobacteriosis cases could be halved if all carcasses would comply with a criterion of <1000 cfu/g neck skin. This review covers Campylobacter contamination of broiler carcasses from transport through the different slaughter steps. Possible intervention methods during slaughter are discussed with a focus on the European situation, where chemicals are not allowed to disinfect carcasses.


Assuntos
Matadouros/normas , Campylobacter/isolamento & purificação , Manipulação de Alimentos/normas , Microbiologia de Alimentos/normas , Aves Domésticas/microbiologia , Animais , Galinhas , Europa (Continente)/epidemiologia , Contaminação de Alimentos/análise , Contaminação de Alimentos/prevenção & controle , Manipulação de Alimentos/métodos , Microbiologia de Alimentos/métodos , Higiene/normas
6.
Food Chem ; 317: 126459, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32113141

RESUMO

The widespread exposure of bisphenol A (BPA) presents a significant risk to human health. A rapid, ultra-sensitive and label-free colorimetric aptasensor using high affinity truncated aptamers was developed for BPA detection. Truncated 38-mer and 12-mer aptamers specific for BPA were obtained through rationally truncation from 63-mer BPA aptamer. The dissociation constants (Kd) of 38-mer and 12-mer aptamers were determined to be 13.17 nM and 27.05 nM. Then, truncated aptamers were used in label-free colorimetric detection assays based on gold nanoparticles (AuNPs). The limit of detections of aptasensors using 38-mer and 12-mer aptamers were 7.60 pM and 14.41 pM, which were 265-fold and 140-fold lower than that of the aptasensor using 63-mer aptamer, respectively. The recovery rates in milk, orange juice and mineralized water samples were 93.88% to 107.30%. Therefore, the developed BPA colorimetric aptasensor using truncated aptamers has great application prospects in food safety control and environmental monitoring.


Assuntos
Aptâmeros de Nucleotídeos/química , Compostos Benzidrílicos/análise , Colorimetria/métodos , Análise de Alimentos/métodos , Fenóis/análise , Animais , Aptâmeros de Nucleotídeos/metabolismo , Compostos Benzidrílicos/química , Compostos Benzidrílicos/metabolismo , Análise de Alimentos/instrumentação , Contaminação de Alimentos/análise , Sucos de Frutas e Vegetais/análise , Ouro/química , Limite de Detecção , Nanopartículas Metálicas/química , Leite/química , Simulação de Acoplamento Molecular , Fenóis/química , Fenóis/metabolismo , Sensibilidade e Especificidade
7.
Food Chem ; 317: 126465, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32114276

RESUMO

Based on the experimental data of gas chromatography-mass spectrometry, an improved artificial neural network was first established to predict the migration of 2-ethylhexyl phthalate (DEHP) plasticizer from poly(vinylidene chloride) (PVDC) into food simulants (ie., heptane, ethanol and water). The sensitivity analysis indicated that temperature acted as a crucial factor influencing the migration values of DEHP. Then, a combined experimental and molecular dynamic (MD) simulation was performed to understand the migration kinetics and the mechanism of DEHP. Hansen solubility parameters of three component (δd, δp, δh) were simplified into two-component solubility parameters (δvdW, δe), and the tuple was successfully applied to describe the interactions between PVDC and food simulants. The MD results showed that high interaction energy and fractional free volume in PVDC/DEHP/food simulant systems accelerated the migration of DEHP. These fundamental studies would provide significant insights into the migration of environmental contaminants.


Assuntos
Dietilexilftalato/análise , Embalagem de Alimentos , Redes Neurais de Computação , Plastificantes/análise , Dietilexilftalato/química , Contaminação de Alimentos/análise , Cromatografia Gasosa-Espectrometria de Massas , Simulação de Dinâmica Molecular , Plastificantes/química , Cloreto de Polivinila/análogos & derivados , Cloreto de Polivinila/química , Solubilidade , Temperatura
10.
PLoS One ; 15(2): e0228624, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32101546

RESUMO

We report a rapid and accurate quantitative detection method using droplet digital PCR (ddPCR) technology to identify cassava adulteration in starch products. The ddPCR analysis showed that the weight of cassava (M) and cassava-extracted DNA content had a significant linear relationship-the correlation coefficient was R2 = 0.995, and the maximum coefficient of variation of replicates was 7.48%. The DNA content and DNA copy number (C) measured by ddPCR also had a linear relationship with R2 = 0.992; the maximum coefficient of variation of replicates was 8.85%. The range of cassava ddPCR DNA content was 25 ng/µL, and the formula M = (C + 32.409)/350.579 was obtained by converting DNA content into the median signal. The accuracy and application potential of the method were verified using the constructed adulteration model.


Assuntos
Análise de Alimentos/métodos , Contaminação de Alimentos , Manihot/química , Reação em Cadeia da Polimerase/métodos , Amido/química , DNA de Plantas/análise , DNA de Plantas/genética , Análise de Alimentos/normas , Manihot/genética , Reação em Cadeia da Polimerase/normas , Amido/normas
11.
Arch Environ Contam Toxicol ; 78(4): 604-621, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32047945

RESUMO

Methylmercury (MeHg) is a neurotoxic pollutant that bioaccumulates and biomagnifies in aquatic food webs, impacting the health of piscivorous wildlife and human consumers of predatory fish. While fish mercury levels have been correlated with various biotic and abiotic factors, many studies only measure adults to characterize the health of locally fished populations, omitting information about how local fish bioaccumulate mercury relative to their growth. In this study, we sought to establish length: total mercury (THg) concentration relationships in juvenile and adult fish of four genera (sunfish, yellow perch, white perch, and killifish) across six freshwater pond systems of Nantucket Island to determine safe consumption sizes across species and environmental conditions. A wide length range (2-21 cm) was utilized to develop linear regression models of ln-THg versus fish length. In most cases, different genera within the same pond indicated similar slopes, supporting that all four genera share comparable features of feeding and growth. Comparing individual species across ponds, differences in ln-THg versus fish length were attributable to known environmental Hg-modulators including surface water MeHg levels, pH, and watershed area. Referencing human health and wildlife criteria, our results confirm that numerous Nantucket freshwater ecosystems contain elevated fish THg levels, which could impact the health of not only piscivorous wildlife in all measured ponds but also recreational fishers in at least two measured systems. Future studies should measure THg levels across juvenile and adult fish to detect potential differences in the slope of THg concentration across fish length relevant for local consumption advice.


Assuntos
Exposição Ambiental/análise , Peixes/crescimento & desenvolvimento , Água Doce/química , Compostos de Metilmercúrio/análise , Alimentos Marinhos/análise , Poluentes Químicos da Água/análise , Adulto , Animais , Ecossistema , Peixes/metabolismo , Cadeia Alimentar , Contaminação de Alimentos/análise , Humanos , Ilhas , Massachusetts , Tanques/química
12.
Chemosphere ; 248: 125989, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32007773

RESUMO

Fish has great socioeconomic, cultural, and nutritional importance for Amazonian populations. Despite all health benefits, fish can accumulate great amounts of mercury (Hg). The entry of Hg in aquatic trophic chains is an issue of concern to animal and human health. Higher risks of human exposure are strongly related to fish consumption. Upper Solimões population has one of the highest fish consumption rates of the Amazon. This study aimed to access the concentration of total Hg (THg) in muscle, liver, and gills of 17 species of fishes marketed in the Upper Solimões Region and Health Risk Assessment. Higher concentrations were observed in Carnivores/Piscivores. The highest THg concentration was found in liver of Cichla ocellaris (4.549 µg/g) and the lowest in gills of Hoplosternum littorale (0.002 µg/g). Most species had higher THg concentrations in muscle>liver>gills, in the Ebb period, and liver>muscle>gills, in the Flood period. Hoplias malabaricus, Pseudoplatystoma fasciatum, Plagioscion squamosissimus, Ageneiosus inermis, and C. ocellaris presented average THg concentrations above the safe limit stablished by WHO. THg levels in C. ocellaris, H. malabaricus, P. squamosissimus, P. fasciatum, and Semaprochilodus insignis were higher than those found in fish of heavily impacted areas. Signs of bioaccumulation and biomagnifications of Hg can already be observed in this region. The Western Amazon Region urgently needs government actions to inhibit Hg release in aquatic ecosystems and to advise this population on the safe amount of fish to be eaten according to species and period of the year.


Assuntos
Monitoramento Ambiental , Peixes/metabolismo , Mercúrio/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , Caraciformes , Ciclídeos , Exposição Dietética/estatística & dados numéricos , Ecossistema , Cadeia Alimentar , Contaminação de Alimentos/estatística & dados numéricos , Brânquias/química , Humanos , Mercúrio/análise , Músculos/química , Medição de Risco , Rios , Alimentos Marinhos , Poluentes Químicos da Água/análise
13.
Sci Total Environ ; 716: 137049, 2020 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-32059313

RESUMO

Food, including rice, is the major source of toxic element cadmium (Cd) for human exposure. Globally, many populations rely on the consumption of significant amounts of rice as a staple food. Using dietary intake and concentration of Cd, this study determines the risk posed to both adults and children due to Cd exposure from consumption of rice sold in Bangladeshi markets. Polished rice samples (n = 144) were collected from the markets of 16 districts of Bangladesh to determine the distribution, regional variability and associated health risk from Cd. The mean and median concentrations of Cd in rice were 44 µg/kg and 34 µg/kg, respectively, ranging between 1 and 180 µg/kg, dry weight. The Cd concentrations of the rice samples did not exceed the safe limit of EU/CODEX but 9% exceeded the safe limit of FSANZ (Food standards Australia New Zealand) values. Results indicated that there were significant variations of Cd among (33%) and within (67%) the districts. Some rice brands such as Najirshail, Katarivogh and Chinigura had Cd levels of 81, 70 and 68 µg/kg, respectively. Cadmium ingested on a daily basis ranged between 0.09 and 0.58 µg/kg body weight (bw) with the incremental lifetime cancer risk (ILCR) for individuals varying between 1.35 × 10-3 and 8.7 × 10-3 in different districts. The age groups (2-5 yrs) and (6-10 yrs) experienced higher risks than others and both males and females were found to be susceptible from Cd exposure of rice.


Assuntos
Oryza , Adulto , Bangladesh , Cádmio , Criança , Pré-Escolar , Feminino , Contaminação de Alimentos , Humanos , Masculino , Medição de Risco
14.
J Agric Food Chem ; 68(7): 2201-2213, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32023042

RESUMO

A feeding study was carried out to investigate the kinetics in cow milk of the 17 polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), the 12 dioxin-like polychlorinated biphenyls (DL-PCBs), and the 6 non-dioxin-like PCBs (NDL-PCBs) regulated by the European (EU) legislation. A fortified ration (ΣPCDD/Fs and DL-PCBs: 24.68 ng TEQ/day/cow; ΣNDL-PCBs: 163.99 µg/day/cow) was given to the animals for 49 days, followed by 42 days on clean feed. EU maximum limit for TEQPCDD/F+DL-PCB was exceeded in milk after 1 week of exposure, while for ΣNDL-PCBs, after 5 weeks. Milk compliance was restored after 1 week on clean feed, but to return to the basal TEQPCDD/F+DL-PCB it took 42 days. At the end of the study, ΣNDL-PCBs had not yet reached the basal level. The carryover rate of ΣNDL-PCBs was 25.4%, while the carryover rate of TEQPCDD/F+DL-PCB was 36.9%. The latter was mainly affected by the 12 congeners contributing most to the toxic equivalent (TEQ) level, explaining the fast overcome of the maximum limit in milk.


Assuntos
Dibenzofuranos/análise , Contaminação de Alimentos/análise , Leite/química , Bifenilos Policlorados/análise , Dibenzodioxinas Policloradas/análise , Ração Animal/análise , Animais , Bovinos , Dioxinas/análise , Feminino
15.
J Agric Food Chem ; 68(8): 2562-2569, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32003990

RESUMO

This study was aimed to evaluate the level of furan and acrylamide contamination in cocoa and noncocoa raw materials, in masses from processing stages, and in chocolates originating from three factories. Acrylamide was determined by the gas chromatography-mass spectrometry (GC-MS) method using the QuEChERS procedure with dispersive solid-phase extraction clean-up and isotopic standard (2,3,3-d3-acrylamide). Furan was analyzed by the headspace solid-phase microextraction/GC-MS technique with the d4-furan marker. Both analytical methods were validated in terms of accuracy, precision, and linearity as well as the limit of detection (LOD) and limit of quantification (LOQ). Among all raw materials, the most abundant in acrylamide were cocoa masses and powders (83.0-127.5 ng g-1). Roasting of cocoa beans increased the content of acrylamide 2-3-fold. The obtained results indicate that acrylamide might be formed during wet conching. Only in cocoa powders and lecithin, it was possible to quantify furan (3.7-10.2 and 16.3 ng g-1, respectively). Roasting of cocoa beans increased the content of furan from

Assuntos
Acrilamida/análise , Cacau/química , Chocolate/análise , Furanos/análise , Acrilamida/isolamento & purificação , Contaminação de Alimentos/análise , Manipulação de Alimentos , Furanos/isolamento & purificação , Cromatografia Gasosa-Espectrometria de Massas , Sementes/química , Microextração em Fase Sólida
16.
J Agric Food Chem ; 68(8): 2554-2561, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32027503

RESUMO

Excessive use of antibiotics in aquatic products is a serious problem for food safety and human health, and on-site detection of antibiotics is highly demanded. Herein, we proposed multivalence aptamer probes, allowing sensitive, label-free, and homogeneous detection of antibiotics in different aquatic products. Compared to commonly used aptamers, multivalence aptamer probes can provide multiple binding sites and a higher affinity for target molecules, and the iterative binding on different binding sites contributes to an amplified recognition effect, sharply increasing the response and sensitivity of aptamer probes. The 2-valence aptamer probes conferred a limit of detection of 0.097 nM for kanamycin detection, where it is estimated that their sensitivity is enhanced 12 times compared to 1-valence aptamer probes. Meanwhile, multivalence aptamer probes allowed us to specifically identify kanamycin among other antibiotics. It could detect kanamycin residual in aquatic products including river eel and puffer fish, as well as tap water with high precision. A multivalence design strategy of aptamer probes would significantly improve the detection performance of aptamers, facilitating the translation of aptamer for food safety control.


Assuntos
Antibacterianos/análise , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Resíduos de Drogas/análise , Alimentos Marinhos/análise , Animais , Aptâmeros de Nucleotídeos/síntese química , Aquicultura , Técnicas Biossensoriais/instrumentação , Peixes/crescimento & desenvolvimento , Contaminação de Alimentos/análise , Água Doce/análise , Canamicina/análise , Limite de Detecção , Poluentes Químicos da Água/análise
17.
Aquat Toxicol ; 221: 105427, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32044545

RESUMO

Local population frequently consumes moray eels and dusky groupers from the Canary Islands. These species are top predators and the interactions between them include predation but also, in some cases, collaborative hunting. These fish are well known to cause ciguatera (CFP) outbreaks in several marine areas such as Japan, Hawaii, French Polynesia and Caribe. Groupers have been involved in CFP events in the Canary Islands, however, moray eels have not yet been well studied in this regard. The present research seeks to describe the finding of a black moray in the stomach of a positive dusky grouper during its necropsy, and to clarify the implication of groupers and moray eels in the food webs, accumulating CTXs in the Canarian environment. The study also updates statistics on the presence of toxic groupers in this archipelago. For these purposes, 248 grouper samples from the CFP official control in the Canary Islands (2018-2019) were analysed and 36 moray eels (5 species) were collected under the EuroCigua project and one was obtained during a dusky grouper necropsy. All samples were analysed with the Neuro-2a cell-based assay (CBA) to evidence CTX-like toxicity. Regarding the necropsied grouper and the moray eel found in its stomach content, the LCMS/MS method allowed the identification and quantification of CCTX1 in both fish at similar levels while none of the P-CTXs for which standards were available were detected. Among groupers, 25.4 % displayed CTX-like toxicity with differences between islands. For moray eels 38.9 % showed toxicity, involving 4 species. Black moray exhibited a high proportion of positives (9/12) and a positive correlation was found between CTX-like toxicity quantification and the black moray weight. Regarding the grouper, and the moray eel found in its stomach, the LCMS/MS method allowed the identification and quantification of C-CTX1 in both fish at similar levels. This found suggests a trophic interaction between these species and their role in maintaining CTXs in the Canary waters where local population commonly demand those species for consumption. The island of El Hierro stands out above all the other Canary Islands with the concerning percentage of positive grouper samples and the high CTX toxicity levels obtained in moray eel specimens analysed in this marine area. This is the first report of CTX-like toxicity in flesh of moray eels fished in the Canary archipelago and the confirmation of the presence of C-CTX1 by LCMS/MS in a black moray from this marine area.


Assuntos
Ciguatoxinas/análise , Enguias/metabolismo , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida , Intoxicação por Ciguatera/epidemiologia , Intoxicação por Ciguatera/etiologia , Ciguatoxinas/toxicidade , Cadeia Alimentar , Contaminação de Alimentos/análise , Conteúdo Gastrointestinal/química , Músculos/química , Alimentos Marinhos/análise , Espanha , Poluentes Químicos da Água/toxicidade
18.
Food Chem ; 317: 126436, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32088404

RESUMO

A zirconium-based metal-organic framework material (UiO-66-OH) was designed and applied for the first time as an adsorbent in food analysis for the solid phase microextraction of trace polybrominated diphenyl ethers (PBDEs) from milk. The materials were characterized by powder X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analyses. Through response surface methodology, the optimal conditions for adsorption were determined to as follows: extraction time of 30 min, ionic strength of 15%, extraction temperature of 61 °C, and stirring speed of 880 r/min. Under the optimal conditions, the method showed excellent linearity with a high correlation coefficient (r ≥ 0.9994), low limits of detection in the range of 0.15-0.35 ng L-1, good interday precision ranging of 7.58%-9.48%, and satisfactory recoveries of 74.7%-118.0%. All these findings showed that the method was reliable and effective for detecting trace PBDEs in milk.


Assuntos
Contaminação de Alimentos/análise , Éteres Difenil Halogenados/isolamento & purificação , Estruturas Metalorgânicas/química , Microextração em Fase Sólida/métodos , Zircônio/química , Adsorção , Animais , Análise de Alimentos/métodos , Éteres Difenil Halogenados/análise , Limite de Detecção , Leite/química , Concentração Osmolar , Microextração em Fase Sólida/instrumentação , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Termogravimetria , Difração de Raios X
19.
Food Chem ; 317: 126427, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32092611

RESUMO

Determination of polyethylene terephthalate (PET) dimer up to heptamer 1st series cyclic oligomers, applying an LC-qTOF-MS method, has been developed and validated. Recoveries ranged between 80 and 112% with RSDs lower than 15%. An innovative semi-quantitative approach has been applied for 2nd and 3rd series cyclic oligomers, using the closest structural-similar 1st series cyclic oligomer standard as analytical reference. Oligomers from the three series were quantified in PET teabags after migration experiments with water and food simulants C (20% v/v ethanol in water) and D1 (50% v/v ethanol in water). No legal migration limits exist currently for these substances. In silico genotoxicity assessment of all identified oligomers has been performed and showed no genotoxicity alert for linear or cyclic molecules. Exposure assessment was performed using EFSA's approach on the total sum of migrating oligomers and on toxicological threshold-of-concern. Amounts found in water were in some cases significantly higher than the respective limits, especially in the worst-case scenario of multiple consumption.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Embalagem de Alimentos , Espectrometria de Massas/métodos , Polietilenotereftalatos/análise , Polietilenotereftalatos/toxicidade , Simulação por Computador , Dimerização , Contaminação de Alimentos/análise , Testes de Mutagenicidade , Polietilenotereftalatos/química , Reprodutibilidade dos Testes , Chá
20.
Food Chem ; 317: 126430, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32092612

RESUMO

Electrochemical Synchronous detection of cadmium (Cd(II)) and lead (Pb(II)) was obtained by acid treated multiwalled carbon nanotube (A-MWCNT) functionalized with hyaluronic acid (Hyalu) and this mixture was separately further modified with l-cysteine (l-Cys) and l-serine (l-Ser). Under the optimized circumstance best voltammetric responses were produced by A-MWCNT/Hyalu/l-Cys and A-MWCNT/Hyalu/l-Ser modified electrodes. The peak current was linearly dependent on the Cd(II) and Pb(II) concentrations in the range from 0.4 to 4 µg L-1. The sensitivities were calculated as 0.7 µA/nM (Cd(II)) and 3.5 µA/nM (Pb(II)) for A-MWCNT/Hyalu/l-Cys/GCE and 0.6 µA/nM (Cd(II)) and 2.6 µA/nM (Pb(II)) for A-MWCNT/Hyalu/l-Ser/GCE. From the calibration plot LODs were calculated to be 0.032 µg L-1 (Cd(II)) and 0.015 µg L-1 (Pb(II)) for A-MWCNT/Hyalu/l-Cys/GCE and 0.057 µg L-1 (Cd(II)) and 0.034 µg L-1 (Pb(II)) for A-MWCNT/Hyalu/l-Ser/GCE. Moreover, the proposed electrodes were subjected to the real sample application in honey, cocos nucifera and egg white.


Assuntos
Cádmio/análise , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Chumbo/análise , Nanocompostos/química , Cádmio/química , Calibragem , Cocos/química , Cisteína/química , Clara de Ovo/análise , Eletrodos , Análise de Alimentos/instrumentação , Mel/análise , Ácido Hialurônico/química , Chumbo/química , Limite de Detecção , Nanotubos de Carbono/química , Sensibilidade e Especificidade , Serina/química
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