Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 8.245
Filtrar
1.
rev.cuid. (Bucaramanga.2010) ; 13(1): 1-20, 20221213.
Artigo em Espanhol | LILACS, BDENF - Enfermagem, COLNAL | ID: biblio-1369171

RESUMO

Introducción: El SARS-CoV-2 es un Betacoronavirus, así como el SARS-CoV y el MERS-CoV, ambos asociados a abortos espontáneos, parto prematuro, morbi-mortalidad materna y alto número de ingresos a UCI en las gestantes. Además, al ser un virus nuevo, se conoce poco sobre los efectos en la gestación. Esta revisión tiene como objetivo analizar la evidencia disponible sobre el SARS-CoV-2 en la gestación. Materiales y métodos: Se realizó una búsqueda de la literatura en PubMed, ProQuest, Scopus, BVS y SciElo. Se realizó la crítica de la evidencia y la extracción de la información con dos instrumentos propuestos por el Instituto Joanna Briggs. Lo anterior bajo las directrices de PRISMA-ScR. Resultados: Se incluyeron 85 artículos que evidenciaron que la mayoría de gestantes con SARS-CoV-2 desarrollaron enfermedad leve a moderada, pero presentaron mayor riesgo de muerte y complicaciones comparado con las pacientes no embarazadas. Se documentó bajo riesgo de transmisión vertical y los resultados perinatales se asociaron a la severidad del cuadro clínico materno. La efectividad del tratamiento no fue concluyente.Discusión: Se discute la presentación clínica de la infección en las gestantes, la transmisión vertical, el tratamiento, la gravedad de la enfermedad y los desenlaces neonatales.Conclusiones: La COVID-19 en la gestación es una complicación que genera mayor morbimortalidad, por lo que es de vital importancia el desarrollo de más investigaciones que amplíen la comprensión de su comportamiento, las implicaciones fisiológicas, emocionales y el posible tratamiento. Esta revisión hace un análisis riguroso de la calidad de los estudios y aporta información valiosa de la evidencia.


Introduction: SARS-CoV-2 is a betacoronavirus as well as SARS-CoV and MERS-CoV, both associated with spontaneous miscarriage, preterm birth, maternal morbidity and mortality and a higher number of ICU admissions for pregnant women. Being a new virus, its effects on pregnancy are little known. This review aims to analyze the available evidence on SARS-CoV-2 in pregnancy. Materials and Methods: A literature review was conducted in PubMed, ProQuest, Scopus, BVS and SciElo. Evidence criticism and information extraction were conducted using two instruments from the Joanna Briggs Institute, following the PRISMA-ScR guidelines. Results: 85 articles were included evidencing that most pregnant women with SARS-COV-2 suffered mild to moderate disease and were at a higher risk of death and complications compared to non-pregnant patients. Low risk of vertical transmission was documented and adverse perinatal outcomes were associated with severe maternal clinical manifestations. The effectiveness of treatment was inconclusive. Discussion: Clinical presentation of infection in pregnant women, vertical transmission, treatment, disease severity and neonatal outcomes were discussed. Conclusions: COVID-19 during pregnancy is a complication that generates greater morbidity and mortality, for which it is vital to develop further research on the understanding of the behavior, physiological and emotional implications and possible treatment. This review makes a rigorous analysis of the quality of studies and provides valuable information from evidence.


Introdução: O SARS-CoV-2 é um betacoronavírus, assim como o SARS-CoV e o MERS-CoV, ambos associados ao aborto espontâneo, parto prematuro, morbidade e mortalidade materna e alto número de internações na UTI em mulheres grávidas. Além disso, sendo um novo vírus, pouco se sabe sobre seus efeitos na gravidez. Esta revisão tem como objetivo analisar as evidências disponíveis sobre o SARS-CoV-2 na gravidez. Materiais e métodos: Foi realizada uma pesquisa bibliográfica na PubMed, ProQuest, Scopus, BVS e SciElo. A analise crítica das provas e a extração de dados foram realizadas utilizando dois instrumentos propostos pelo Instituto Joanna Briggs sob as diretrizes do PRISMA-ScR. Resultados: 85 artigos foram incluídos, mostrando que a maioria das mulheres grávidas com SRA-CoV-2 desenvolveu doença leve a moderada, mas tinha um risco maior de morte e complicações em comparação com pacientes não grávidas. Baixo risco de transmissão vertical foi documentado e os resultados perinatais foram associados à gravidade do quadro clínico materno. A eficácia do tratamento foi inconclusiva. Discussão: Apresentação clínica da infecção em mulheres grávidas, transmissão vertical, tratamento, gravidade da doença e resultados neonatais são discutidos. Conclusões: A COVID-19 na gravidez é uma complicação que gera maior morbidade e mortalidade, portanto, mais pesquisas para expandir a compreensão de seu comportamento, implicações fisiológicas e emocionais, e o tratamento potencial são de vital importância. Esta revisão fornece uma análise rigorosa da qualidade dos estudos e informações valiosas a partir das evidências.


Assuntos
Humanos , Masculino , Feminino , Recém-Nascido , Gravidez , Coronavirus , SARS-CoV-2 , COVID-19
2.
Ciudad de Panamá; OPS; 2022-08-03.
em Espanhol | PAHO-IRIS | ID: phr2-56223

RESUMO

Hasta el 30 de julio, se reportan 7.743 pacientes (0,8%) en aislamiento domiciliario, 163 pacientes (0,0%) se encuentran hospitalizados (132 en sala general y 31 en Unidades de Cuidado Intensivo - UCI). Se informan 938.038 (98,3%) casos como recuperados. Se notifica un total de 8.425 fallecidos con 41 nuevas muertes desde el 8 de julio, para un porcentaje de fallecidos de 0,9%.


Assuntos
COVID-19 , Coronavirus , SARS-CoV-2 , Infecções por Coronavirus , Betacoronavirus , Análise de Situação , Emergências , América , Panamá
3.
Brasilia, D.F.; OPAS; 2022-08-02. (OPAS-W/BRA/PHE/COVID-19/22-0025).
Não convencional em Português | PAHO-IRIS | ID: phr2-56210

RESUMO

Este documento tem como objetivo descrever um conjunto mínimo de atividades de vigilância recomendadas em nível nacional para detectar e monitorar a prevalência relativa de variantes do SARS-CoV-2 e delinear um conjunto de atividades para a caracterização e avaliação do risco apresentado por essas variantes. Também é fornecido um conjunto de indicadores para padronizar o monitoramento e a divulgação pública da circulação de variantes. O documento destina-se principalmente às autoridades de saúde pública nacionais e subnacionais e aos parceiros que apoiam a implementação da vigilância de variantes do SARS-CoV-2. Orientações adicionais foram publicadas para as partes interessadas laboratoriais sobre testes diagnósticos para SARS-CoV-2 e sequenciamento para objetivos de saúde pública, com um guia de implementação para sequenciamento do SARS-CoV-2.


Assuntos
SARS-CoV-2 , COVID-19 , Vacinas contra COVID-19 , COVID-19 , Coronavirus , Infecções por Coronavirus
5.
Philos Trans A Math Phys Eng Sci ; 380(2233): 20210301, 2022 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-35965470

RESUMO

We present a method for rapid calculation of coronavirus growth rates and [Formula: see text]-numbers tailored to publicly available UK data. We assume that the case data comprise a smooth, underlying trend which is differentiable, plus systematic errors and a non-differentiable noise term, and use bespoke data processing to remove systematic errors and noise. The approach is designed to prioritize up-to-date estimates. Our method is validated against published consensus [Formula: see text]-numbers from the UK government and is shown to produce comparable results two weeks earlier. The case-driven approach is combined with weight-shift-scale methods to monitor trends in the epidemic and for medium-term predictions. Using case-fatality ratios, we create a narrative for trends in the UK epidemic: increased infectiousness of the B1.117 (Alpha) variant, and the effectiveness of vaccination in reducing severity of infection. For longer-term future scenarios, we base future [Formula: see text] on insight from localized spread models, which show [Formula: see text] going asymptotically to 1 after a transient, regardless of how large the [Formula: see text] transient is. This accords with short-lived peaks observed in case data. These cannot be explained by a well-mixed model and are suggestive of spread on a localized network. This article is part of the theme issue 'Technical challenges of modelling real-life epidemics and examples of overcoming these'.


Assuntos
Coronavirus , Epidemias , Epidemias/prevenção & controle , Reprodução , Reino Unido/epidemiologia
6.
Rev. esp. enferm. dig ; 114(8): 448-454, agosto 2022. tab, graf
Artigo em Inglês | IBECS | ID: ibc-205700

RESUMO

Background: the impact of the COVID-19 outbreak and lockdown on liver transplant (LT) patients remains unknown. The aim of this cross-sectional study was to assess the consequences of the COVID-19 pandemic on the physical and mental health of LT patients during the lockdown period.Methods: a web-based questionnaire was emailed to 238 LT patients undergoing regular follow-up at our unit between August and October 2020. This pseudonymized survey explored demographic and lifestyle variables (i.e., eating and physical habits), disruptions in routine medical care, different dimensions of mental health, COVID-19-related mood and coping (worries/anxiety, depression, insomnia, fear of COVID, resilience, etc.) and health perception using different validated instruments.Results: altogether, 48.7 % (116 of 238) LT recipients accepted to participate in the study, 104 of whom gave their consent to publish the data. The median age was 63 years. Up to 39.4 % presented worrying scores indicating moderate/severe generalized anxiety disorder (GAD), whereas 25.5 % exhibited moderate/severe insomnia and only 10.5 % moderate/severe depression. Forty patients (38.5 %) gained weight, 24 % experienced a worsening in their eating habits and 63.4 % referred to practicing less or much less exercise during the lockdown. Only 25 % perceived a worsening in the control of their chronic comorbidities. Missed medical appointments (0.9 %) or poor adherence to therapy (1.9 %) were exceptional.Conclusions: COVID-19 lockdown has negatively impacted the mental and physical health of LT patients. Long-term consequences remain unclear. (AU)


Assuntos
Humanos , Ansiedade/epidemiologia , Ansiedade/psicologia , Coronavirus , Controle de Doenças Transmissíveis , Transplante de Fígado , Estudos Transversais , Pandemias
7.
Rev. esp. enferm. dig ; 114(8): 455-460, agosto 2022. tab, graf
Artigo em Inglês | IBECS | ID: ibc-205701

RESUMO

Background: the COVID-19 pandemic has impacted on several aspects of health care services worldwide. The aim of the study was to determine its influence on the case volume, success rate and complication rate of endoscopic retrograde cholangiopancreatography (ERCP).Method: all patients who underwent ERCP one-year before and after applying COVID-19 safety measures at the Qena University Hospital were included. Data were collected from the patients' records, analyzed and compared.Results: a total of 250 patients underwent ERCP between April 1st, 2019 and March 31st, 2021, and the mean age of participants was 52 ± 18 years. There was a 5 % increase in case volume after applying COVID-19 safety measures (128 vs 122) and the total procedure time was significantly shorter (42 vs 46 minutes, p = 0.04). There was no significant difference in the overall success rate and complication rate. Procedure success significantly correlated with cannulation attempts and total procedure time in both groups, and serum bilirubin and cannulation time in the pre-COVID-19 patients and alkaline phosphatase (ALP) in post-COVID patients. ERCP-related complications significantly correlated with cannulation attempts in both groups, and ALP, international normalized ratio (INR), cannulation time and total procedure time in pre-COVID-19 patients, and platelet count and amylase in post-COVID patients. Two patients were confirmed COVID-19 cases at the time of ERCP; therapeutic targets were achieved in both with a smooth post-ERCP recovery. Three out of nine ERCP team members caught a mild to moderate COVID-19 infection and recovered after receiving proper management.Conclusion: our result show that there was no negative impact of using COVID-19 safety measures and precautions on the case-volume, indications, overall outcome or complication rate of ERCP. (AU)


Assuntos
Humanos , Fosfatase Alcalina , Coronavirus , Cateterismo/métodos , Colangiopancreatografia Retrógrada Endoscópica/métodos , Pandemias
9.
PLoS One ; 17(7): e0271039, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35816483

RESUMO

We apply the new GenomeBits method to uncover underlying genomic features of omicron and delta coronavirus variants. This is a statistical algorithm whose salient feature is to map the nucleotide bases into a finite alternating (±) sum series of distributed terms of binary (0,1) indicators. We show how by this method, distinctive signals can be uncovered out of the intrinsic data organization of amino acid progressions along their base positions. Results reveal a sort of 'ordered' (or constant) to 'disordered' (or peaked) transition around the coronavirus S-spike protein region. Together with our previous results for past variants of coronavirus: Alpha, Beta, Gamma, Epsilon and Eta, we conclude that the mapping into GenomeBits strands of omicron and delta variants can help to characterize mutant pathogens.


Assuntos
Coronavirus , Coronavirus/genética , Glicoproteína da Espícula de Coronavírus/genética
11.
Front Immunol ; 13: 912717, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35784331

RESUMO

We present evidence suggesting that the severe acute respiratory syndrome (SARS) coronavirus non-structural protein 13 (Nsp13) modulates the Z-RNA dependent regulated cell death pathways . We show that Z-prone sequences [called flipons] exist in coronavirus and provide a signature (Z-sig) that enables identification of the animal viruses from which the human pathogens arose. We also identify a potential RIP Homology Interaction Motif (RHIM) in the helicase Nsp13 that resembles those present in proteins that initiate Z-RNA-dependent cell death through interactions with the Z-RNA sensor protein ZBP1. These two observations allow us to suggest a model in which Nsp13 down regulates Z-RNA activated innate immunity by two distinct mechanisms. The first involves a novel ATP-independent Z-flipon helicase (flipase) activity in Nsp13 that differs from that of canonical A-RNA helicases. This flipase prevents formation of Z-RNAs that would otherwise activate cell death pathways. The second mechanism likely inhibits the interactions between ZBP1 and the Receptor Interacting Proteins Kinases RIPK1 and RIPK3 by targeting their RHIM domains. Together the described Nsp13 RHIM and flipase activities have the potential to alter the host response to coronaviruses and impact the design of drugs targeting the Nsp13 protein. The Z-sig and RHIM domains may provide a way of identifying previously uncharacterized viruses that are potentially pathogenic for humans.


Assuntos
Infecções por Coronavirus , Coronavirus , Vírus da SARS , Síndrome Respiratória Aguda Grave , Animais , Coronavirus/metabolismo , DNA Helicases/metabolismo , RNA , Vírus da SARS/metabolismo
12.
Turk J Pediatr ; 64(3): 500-509, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35899563

RESUMO

BACKGROUND: Human coronaviruses (HCoVs) cause a comprehensive clinic ranging from asymptomatic course to pneumonia. We aimed to describe the HCoV infections in children to determine the clinical status and coinfection effects in a five-year retrospective surveillance study. The primary outcome was admission to the intensive care unit (ICU) and the secondary outcome was the need of high oxygen support. METHODS: Between September 2015 and November 2020, all patients whose reverse transcription polymerase chain reaction (RT-PCR) tests were positive were determined and patients with HCoVs were included in the study. Demographical characteristics, underlying chronic diseases, clinical diagnosis, laboratory data, subtypes of HCoVs, radiological findings, treatments, hospitalization, and ICU admission were analyzed. RESULTS: Of the 2606 children, the overall respiratory tract virus detection rate was 82.4%. Among these, 98 cases were HCoVs positive and of these 80 (81.6%) were under five years of age and most of the patients were admitted to the hospital in spring and 70% were a mixed infection with other respiratory viruses. Since lower respiratory tract infections are more common in HCoV coinfections, a significant difference was found in clinical diagnosis (p < 0.001). The presence of hypoxia (p=0.003) and underlying disease (p=0.004) were found to be significantly more common in patients admitted to the ICU. The presence of hypoxia, infiltration on chest X-ray, and elevated C-reactive protein levels were more frequently determined in patients who received high oxygen support (p=0.001, p=0.036, p=0.004, respectively). CONCLUSIONS: Clinical findings may be more severe if HCoVs, which generally cause mild respiratory disease, are coinfected with another viral agent.


Assuntos
Infecções por Coronavirus , Coronavirus , Infecções Respiratórias , Criança , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/terapia , Humanos , Hipóxia/etiologia , Lactente , Oxigênio , Infecções Respiratórias/epidemiologia , Estudos Retrospectivos , Estações do Ano
13.
Viruses ; 14(7)2022 07 14.
Artigo em Inglês | MEDLINE | ID: mdl-35891517

RESUMO

A PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex real-time RT-PCR was developed and validated for the simultaneous detection and differentiation of four swine enteric coronaviruses (PEDV, PDCoV, TGEV and SADS-CoV) in one PCR reaction (XIPC serves as an exogenous internal positive control). The 5-plex PCR had excellent analytical specificity, analytical sensitivity, and repeatability based on the testing of various viral and bacterial pathogens, serial dilutions of virus isolates, and in vitro transcribed RNAs. The 5-plex PCR had comparable diagnostic performance to a commercial PEDV/TGEV/PDCoV reference PCR, based on the testing of 219 clinical samples. Subsequently, 1807 clinical samples collected from various U.S. states during 2019-2021 were tested by the 5-plex PCR to investigate the presence of SADS-CoV in U.S. swine and the frequency of detecting swine enteric CoVs. All 1807 samples tested negative for SADS-CoV. Among the samples positive for swine enteric CoVs, there was a low frequency of detecting TGEV, an intermediate frequency of detecting PDCoV, and a high frequency of detecting PEDV. Although there is no evidence of SADS-CoV presence in the U.S. at present, the availability of the 5-plex PCR will enable us to conduct ongoing surveillance to detect and differentiate these viruses in swine samples and other host species samples as some of these coronaviruses can cause cross-species infection.


Assuntos
Infecções por Coronavirus , Coronavirus , Vírus da Diarreia Epidêmica Suína , Doenças dos Suínos , Alphacoronavirus , Animais , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/veterinária , Fezes , Vírus da Diarreia Epidêmica Suína/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Suínos , Doenças dos Suínos/diagnóstico
14.
Viruses ; 14(7)2022 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-35891531

RESUMO

Four seasonal human coronaviruses (sHCoVs) are endemic globally (229E, NL63, OC43, and HKU1), accounting for 5-30% of human respiratory infections. However, the epidemiology and evolution of these CoVs remain understudied due to their association with mild symptomatology. Using a multigene and complete genome analysis approach, we find the evolutionary histories of sHCoVs to be highly complex, owing to frequent recombination of CoVs including within and between sHCoVs, and uncertain, due to the under sampling of non-human viruses. The recombination rate was highest for 229E and OC43 whereas substitutions per recombination event were highest in NL63 and HKU1. Depending on the gene studied, OC43 may have ungulate, canine, or rabbit CoV ancestors. 229E may have origins in a bat, camel, or an unsampled intermediate host. HKU1 had the earliest common ancestor (1809-1899) but fell into two distinct clades (genotypes A and B), possibly representing two independent transmission events from murine-origin CoVs that appear to be a single introduction due to large gaps in the sampling of CoVs in animals. In fact, genotype B was genetically more diverse than all the other sHCoVs. Finally, we found shared amino acid substitutions in multiple proteins along the non-human to sHCoV host-jump branches. The complex evolution of CoVs and their frequent host switches could benefit from continued surveillance of CoVs across non-human hosts.


Assuntos
Infecções por Coronavirus , Coronavirus , Infecções Respiratórias , Animais , Coronavirus/genética , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/veterinária , Cães , Humanos , Camundongos , Coelhos , Estações do Ano , Análise de Sequência de DNA
15.
Viruses ; 14(7)2022 07 16.
Artigo em Inglês | MEDLINE | ID: mdl-35891537

RESUMO

In this review, we explore recombination in two very different virus families that have become major threats to human health. The Herpesviridae are a large family of pathogenic double-stranded DNA viruses involved in a range of diseases affecting both people and animals. Coronaviridae are positive-strand RNA viruses (CoVs) that have also become major threats to global health and economic stability, especially in the last two decades. Despite many differences, such as the make-up of their genetic material (DNA vs. RNA) and overall mechanisms of genome replication, both human herpes viruses (HHVs) and CoVs have evolved to rely heavily on recombination for viral genome replication, adaptation to new hosts and evasion of host immune regulation. In this review, we will focus on the roles of three viral exonucleases: two HHV exonucleases (alkaline nuclease and PolExo) and one CoV exonuclease (ExoN). We will review the roles of these three nucleases in their respective life cycles and discuss the state of drug discovery efforts against these targets.


Assuntos
Infecções por Coronavirus , Coronavirus , Animais , Antivirais/farmacologia , Antivirais/uso terapêutico , Coronavirus/genética , Descoberta de Drogas , Exonucleases , Humanos , Mutação , Recombinação Genética , Simplexvirus , Replicação Viral
16.
Med Care ; 60(8): 596-601, 2022 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-35797458

RESUMO

BACKGROUND: During the coronavirus disease-2019 (COVID-19) pandemic cumulative United States COVID-19 deaths per capita were higher than all other large, high-income nations, but with substantial variation across the country. OBJECTIVE: The aim was to detail the public health response during the pandemic in Eagle County, Colorado. RESEARCH DESIGN AND MEASURES: Observational study using pre-COVID-19 county public health metrics. Pandemic actions were recorded from a narrative summary of daily phone consultations by a county-wide taskforce and interviews. Outcomes obtained from local, state, and nationally reported databases. METHODS: Eagle County began with a life expectancy of 85.9, low all-cause age-adjusted death rates equal for both White and Latinx populations, a high household median income, and other prepandemic advantages. It also launched an innovative, independent county-wide taskforce lead by experienced mid-level managers. The taskforce implemented rapid communication of decision consequences, made immediate course corrections without traditional organizational approvals or contradictory political pressures. RESULTS: Eagle County was first in Colorado to obtain Personal Protective Equipment and to establish a drive-through testing facility. The COVID-19 case fatality rate was 0.34%. The sole intensive care unit never reached maximum capacity. By March 2022, Eagle County had administered at least 1 vaccine dose to 100% of the population and 83% were fully vaccinated. CONCLUSIONS: It is not possible to directly attribute superior outcomes to either the baseline characteristics of Eagle County or its innovative taskforce design and deployment. Rather this report highlights the potential impact that improving the baseline health status of US citizens and permitting novel problem-solving approaches by local public health officials might have for the next pandemic.


Assuntos
COVID-19 , Coronavirus , Águias , Animais , COVID-19/epidemiologia , Colorado , Humanos , Pandemias/prevenção & controle , Saúde Pública , Estados Unidos/epidemiologia
17.
Tohoku J Exp Med ; 258(1): 63-68, 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35858800

RESUMO

The detailed clinical course of coronavirus disease 2019 (COVID-19) in patients with hairy cell leukemia (HCL) is rarely reported. We report the first case of HCL diagnosed with prolonged pancytopenia after COVID-19 infection in Japan. We describe the case of a 56-year-old man who was diagnosed with COVID-19. Computed tomography revealed ground-glass opacities in the bilateral lung lobes as well as splenomegaly. Remdesivir and dexamethasone were administered for the treatment of COVID-19. Since the pancytopenia persisted, bone marrow examination was performed, and he was diagnosed with HCL. Although pancytopenia can occur with COVID-19 alone, clinicians should be alerted regarding the presence of hematologic malignancies in patients in whom pancytopenia persists after COVID-19 treatment or in those with splenomegaly. Further, the condition of all previously reported patients with COVID-19 associated with HCL was severe enough to require mechanical ventilation. This is the first case in which the disease was not severe. The interleukin-6 (IL-6) level was lower in this case than in previous cases, suggesting that racial differences in IL-6 production may have contributed to COVID-19 severity.


Assuntos
COVID-19 , Coronavirus , Leucemia de Células Pilosas , Pancitopenia , COVID-19/complicações , COVID-19/tratamento farmacológico , Humanos , Interleucina-6 , Leucemia de Células Pilosas/complicações , Leucemia de Células Pilosas/tratamento farmacológico , Leucemia de Células Pilosas/patologia , Masculino , Pessoa de Meia-Idade , Pancitopenia/complicações , Esplenomegalia/complicações , Esplenomegalia/patologia
18.
Clin Geriatr Med ; 38(3): 501-517, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35868669

RESUMO

Patients with cognitive impairment have paid a heavy price for the coronavirus disease 2019 pandemic. Their clinical characteristics and their place of life made them particularly exposed to being infected and suffering from severe forms. The repercussions of the isolation measures also had significant repercussions on the expression of their neuropsychiatric symptoms and the burden on families and health care professionals.


Assuntos
COVID-19 , Disfunção Cognitiva , Coronavirus , Idoso , Disfunção Cognitiva/diagnóstico , Pessoal de Saúde , Humanos , Pandemias
19.
Sci Rep ; 12(1): 13118, 2022 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-35908082

RESUMO

The pandemic caused by severe acute respiratory Coronavirus-2 (SARS-CoV-2) has been ongoing for over two years, and treatment for COVID-19, other than monoclonal antibodies, is urgently required. Accordingly, we have investigated the inhibitors of SARS-CoV-2 protein targets by high-throughput virtual screening using a marine natural products database. Considering the calculated molecular properties and availability of the compounds, (+)-usnic acid was selected as a suitable hit. In the in vitro antiviral assay of (+)-usnic acid by the immunofluorescence method, IC50 was 7.99 µM, which is similar to that of remdesivir used as a positive control. The generalized Born and surface area continuum solvation (MM/GBSA) method was performed to find the potent target of (+)-usnic acid, and the Mpro protein showed the most prominent value, -52.05 kcal/mol, among other SARS-CoV-2 protein targets. Thereafter, RMSD and protein-ligand interactions were profiled using molecular dynamics (MD) simulations. Sodium usnate (NaU) improved in vitro assay results with an IC50 of 5.33 µM and a selectivity index (SI) of 9.38. Additionally, when (+)-usnic acid was assayed against SARS-CoV-2 variants, it showed enhanced efficacy toward beta variants with an IC50 of 2.92 µM and SI of 11.1. We report the in vitro anti-SARS-CoV-2 efficacy of (+)-usnic acid in this study and propose that it has the potential to be developed as a COVID-19 treatment if its in vivo efficacy has been confirmed.


Assuntos
COVID-19 , Infecções por Coronavirus , Coronavirus , Benzofuranos , COVID-19/tratamento farmacológico , Proteases 3C de Coronavírus , Humanos , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Inibidores de Proteases/farmacologia , SARS-CoV-2 , Sais
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...