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1.
Science ; 367(6478): 628-629, 2020 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-32029616
3.
J Photochem Photobiol B ; 203: 111774, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31931386

RESUMO

Zeolitic imidazole framework (ZIF) is an emerging class of metal organic frameworks exhibiting unique features such as crystalline nature with tunable pore size, large surface area and biocompatible nature. Exceptional thermal and chemical stabilities of ZIF-L make it a suitable candidate for biomedical applications. The present study has focused on the single step fabrication of catechin encapsulated ZIF-L and evaluation of its antibiofilm efficiency, larvicidal activity and dye degradation ability. The as- prepared CA@ZIF-L nanocomposite was characterized by spectroscopic and microscopic techniques. The results revealed that the CA@ZIF-L showed significant toxicity against mosquito larvae in a dose dependent manner with the IC50 63.43±1.25 µg/mL. CA@ZIF-L showed dose dependent reduction of biofilm formation in both ATCC and clinical MRSA strains. In addition, CA@ZIF-L exhibited excellent photocatalytic activity with around 92% degradation of methylene blue under direct sunlight. Overall, the present work highlights the possibility of employing the multifunctional CA@ZIF-L nanocomposite as a suitable material for biomedical and photocatalytic applications.


Assuntos
Biofilmes/efeitos dos fármacos , Catequina/química , Estruturas Metalorgânicas/química , Nanocompostos/toxicidade , Zeolitas/química , Animais , Catálise , Culicidae/efeitos dos fármacos , Culicidae/crescimento & desenvolvimento , Imidazóis/química , Larva/efeitos dos fármacos , Luz , Staphylococcus aureus Resistente à Meticilina/fisiologia , Azul de Metileno/química , Nanocompostos/química , Tamanho da Partícula , Fotólise/efeitos da radiação
4.
Artigo em Português | LILACS, Sec. Est. Saúde SP, SESSP-CTDPROD, Sec. Est. Saúde SP | ID: biblio-1049862

RESUMO

Recentemente o Brasil sofreu com diversas epidemias de diferentes arboviroses, acometendo milhares de pessoas. Devido a isso, as autoridades de saúde pública têm realizado estudos de novas estratégias de combate ao mosquito Aedes aegypti, visando diminuir o número de pessoas afetadas por essas doenças, tanto por medidas de controle da infestação, quanto por medidas que visam diminuir o contato homem-vetor. Esse estudo teve como objetivo analisar fêmeas de Aedes aegypti classificadas como estágio 1 de Sella e evidenciar sua importância como parâmetro de avaliação de estratégias de diminuição do contato homem-vetor. O método utilizado para investigar a proporção de fêmeas que se alimentaram de sangue humano foi o ensaio imunoenzimático (ELISA). As fêmeas de mosquitos processadas nesse estudo foram capturadas em 3 regiões distintas da cidade de Marília­SP. Os ensaios evidenciaram a importância do processamento de fêmeas em todos os graus de digestão sanguínea, pois mesmo as que visualmente não continham a presença de sangue em seu abdômen apresentaram alto percentual de positividade para sangue humano. Demonstra-se que a utilização da proporção de fêmeas alimentadas com sangue humano pode ser um índice interessante para avaliar estratégias protetivas de diminuição do contato homem-vetor.


Assuntos
Infecções por Arbovirus , Aedes , Culicidae
5.
Rev Soc Bras Med Trop ; 53: e20190222, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31859948

RESUMO

INTRODUCTION: The genus Haemagogus Williston is restricted to Central America and North and middle of South America and it includes numerous species of yellow fever virus vectors. METHODS: Adult female and larvae mosquitoes were collected using hand aspirators and dipper and pipette, respectively. RESULTS: The first record of a species of Haemagogus and particularly of Haemagogus spegazzinii was from La Pampa, Argentina. With this registry, the number of species found in La Pampa province rises to 18. CONCLUSIONS: New information on breeding sites for the species and implications of this new record suggest a possible extension of distribution in the near future.


Assuntos
Culicidae/classificação , Mosquitos Vetores/classificação , Animais , Argentina , Feminino , Febre Amarela/transmissão
7.
Zootaxa ; 4608(2): zootaxa.4608.2.3, 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-31717146

RESUMO

Eleven species of Uranotaenia, one belonging to the subgenus Pseudoficalbia and ten to the subgenus Uranotaenia, have been recorded in Mexico. In general, two species are commonly recorded in urban areas by the vector surveillance programs, but the other species are rarely collected as they occupy habitats in non-anthropized environments, and for that reason they remain poorly known. Some of the species recorded in Mexico have not been described in the pupal stage and other developmental stages have only been partially described, making their recognition difficult. One of these species is Uranotaenia (Uranotaenia) coatzacoalcos Dyar Knab, as a complete description of its larval chaetotaxy is not available, and the pupa has not been described. In this report, we provide a complete morphological description of the fourth-instar larva, the first description of the pupa, and a review of the female and male diagnostic characteristics useful for recognition of the species. We also provide keys in English and Spanish for the recognition of the larvae, pupae, females, and male genitalia of the species of Uranotaenia recorded in Mexico.


Assuntos
Culicidae , Animais , Feminino , Genitália Masculina , Larva , Masculino , México , Mosquitos Vetores , Pupa
8.
Zootaxa ; 4691(3): zootaxa.4691.3.9, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31719397

RESUMO

A new species, Paraedes jambulingami, is described from Assam, northeastern India. The adult male and female are described in detail and an illustration of the male is provided. This species is easily distinguished from all known species of the genus Paraedes by the presence of broad silvery or golden scales on the paratergite and the outer arm of the basal mesal lobe of the male genitalia bearing a long, flattened blade-like seta. Dichotomous keys are provided for the identification of the adult females and males of the five species of the genus that are known to occur in India.


Assuntos
Aedes , Culicidae , Dípteros , Animais , Cor , Feminino , Genitália Masculina , Índia , Masculino
9.
Zootaxa ; 4576(3): zootaxa.4576.3.7, 2019 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-31715752

RESUMO

Culiseta ochroptera is a rare mosquito species in Germany, for which little ecological data are available. It is a species of peatlands where it occurs in a variety of habitats in different mire types. These landscape elements, strongly reduced and degraded by human use, are now often protected areas. Within the scope of a Germany-wide mosquito monitoring program, Cs. ochroptera was documented several times from 2011 to 2017, complementing historical data about its distribution and ecology. The records in northern and eastern Germany represent different types of peatlands and aquatic sites. Highest seasonal population densities were recorded in July and August, caused by the second of at least two annual generations. The morphological variants of Cs. ochroptera as described in the past were assigned to three forms (light, normal, dark), and the previously noted but neglected presence of postspiracular scales is recognized as a useful character supporting species identification.


Assuntos
Culicidae , Animais , Ecologia , Ecossistema , Alemanha , Humanos , Densidade Demográfica
10.
Zootaxa ; 4576(1): zootaxa.4576.1.7, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31715778

RESUMO

Description of the pupa and redescriptions of the larva, female, male and male genitalia of Toxorhynchites (Lynchiella) moctezuma (Dyar Knab) are presented based on specimens from Mexico, providing information that helps to distinguish this species from other species within the subgenus Lynchiella. Additionally, we report the first finding of Tx. (Lyn.) grandiosus (Williston) in the state of Veracruz, Mexico.


Assuntos
Culicidae , Animais , Feminino , Larva , Masculino , México , Pupa
11.
Zootaxa ; 4586(2): zootaxa.4586.2.10, 2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-31716136

RESUMO

The original description of Topomyia aureoventer by Theobald (1910) included only the adult female. Description of the adult male, pupa and larva, with figures of the male genitalia, pupa and larva, are provided. The larva is predacious with well-developed mandibles and inhabits leaf axils of Colocasia plants.


Assuntos
Culicidae , Animais , Feminino , Genitália Masculina , Índia , Larva , Masculino , Pupa
12.
Zootaxa ; 4624(3): zootaxa.4624.3.9, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31716216

RESUMO

Accurate identification of mosquitoes is crucial for the management and control of vector species. Although Singapore is a tropical country with high mosquito diversity, there are no identification keys specific to its mosquito fauna. An illustrated identification key to the genera of the adult female mosquitoes of Singapore is presented. Twenty genera are characterized and two genera found in Malaysia, Culiseta and Topomyia, are also included. The key will aid non-taxonomically trained operational officers in adult mosquito identification. The key to the genera provides the first crucial step in the process to species identification.


Assuntos
Culicidae , Animais , Vetores de Doenças , Feminino , Malásia , Mosquitos Vetores , Singapura
13.
Zootaxa ; 4565(1): zootaxa.4565.1.13, 2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-31716499

RESUMO

The ultrastructure and morphometry of the egg of Coquillettidia albifera (Prado) (Diptera: Culicidae) studied under scanning electronic microscopy is presented for the first time. The identification of the species was confirmed by examination of the genitalia of males collected with females. The male genitalia are redescribed and digital photographs are provided. The eggs are elliptical with the anterior region elongate and tubuliform, length 661 ± 2.59 µm and width 183 ± 2.59 µm (ratio length/width 3.61 ± 0.08). The dorsal surface is covered by a hexagonal, pentagonal and rectangular chorionic network with randomly distributed tubercles. The micropylar disc has a diameter of 18.91 ± 0.65 µm, surrounded by a continuous protuberant collar with a thickness of 4.4 ± 0.3 µm. No structure or vestige of cement related to egg attachment was observed.


Assuntos
Culicidae , Animais , Córion , Feminino , Genitália Masculina , Masculino , Microscopia Eletrônica de Varredura , Óvulo
14.
Zootaxa ; 4658(3): zootaxa.4658.3.5, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31716733

RESUMO

Twenty-five species of Tortricidae from Bhutan are discussed, 14 of which are recorded for the first time: Acleris perfundana Kuznetsov, 1962, Archips limatus Razowski, 1977, Chirapsina expleta (Meyrick, 1923), Clepsis humana (Meyrick, 1912), Adoxophyes privatana (Walker, 1863), Lumaria probolias (Meyrick, 1907), Meridemis bathymorpha Diakonoff, 1976, Isodemis illiberalis Meyrick, 1918, Lobesia ambigua Diakonoff, 1954, Metendothenia ordospina Jirasuttayaporn Pinkeaw, 2018, Gibberifera glaciata (Meyrick, 1907), Crocidosema plebejana Zeller, 1847, Lepteucosma charassuncus Razowski, 2006, and Microsarotis bicincta Diakonoff, 1976. Females of Chirapsina expleta and Epiblema charadrias Diakonoff, 1977 are described and illustrated for the first time. Nine new species are described: Eupoecilia jakarana, sp. nov., E. gedui, sp. nov., Lumaria phuntschona, sp. nov., Borneogena trashiyana, sp. nov., Bactra cophinana, sp. nov., Penthostola subnigrantis, sp. nov., M. brunnofasciana, sp. nov., Peridaedala nigrifasciana, sp. nov., and Epiblema albulusana, sp. nov. Adults and their genitalia are illustrated.


Assuntos
Culicidae , Lepidópteros , Mariposas , Distribuição Animal , Animais , Butão , Feminino , Genitália
15.
Zootaxa ; 4656(2): zootaxa.4656.2.8, 2019 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-31716829

RESUMO

The mosquito genus Wyeomyia (Diptera: Culicidae) includes 17 subgenera and 140 species, 30 of which are without subgeneric placement. The taxonomic history of the unplaced species is summarized and the validity of Wy. compta Senevet Abonnenc is discussed based on examination of the type material. It is clear that the description of Wy. compta was based on specimens belonging to two different species. The holotype adult female is identical to Wy. argenteorostris (Bonne-Wepster Bonne) whereas the characters described from a larval exuviae mounted on the same slide with the holotype of Wy. albocaerulea Senevet Abonnenc coincide with those which characterize the subgenus Dodecamyia. Since the larval exuviae cannot be definitely associated with the holotype female and it belongs to either Wy. aphobema Dyar or another species of the subgenus Dodecamyia, it cannot be afforded type status. Therefore, Wy. compta is synonymized with Wy. argenteorostris.


Assuntos
Culicidae , Animais , Feminino , Larva
16.
Parasit Vectors ; 12(1): 462, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31578155

RESUMO

BACKGROUND: Assessing adult mosquito populations is an important component of disease surveillance programs and ecosystem health assessments. Inference from adult trapping datasets involves comparing populations across space and time, but comparisons based on different trapping methods may be biased if traps have different efficiencies or sample different subsets of the mosquito community. METHODS: We compared four widely-used trapping methods for adult mosquito data collection in Kruger National Park (KNP), South Africa: Centers for Disease Control miniature light trap (CDC), Biogents Sentinel trap (BG), Biogents gravid Aedes trap (GAT) and a net trap. We quantified how trap choice and sampling effort influence inferences on the regional distribution of mosquito abundance, richness and community composition. RESULTS: The CDC and net traps together collected 96% (47% and 49% individually) of the 955 female mosquitoes sampled and 100% (85% and 78% individually) of the 40 species or species complexes identified. The CDC and net trap also identified similar regional patterns of community composition. However, inference on the regional patterns of abundance differed between these traps because mosquito abundance in the net trap was influenced by variation in weather conditions. The BG and GAT traps collected significantly fewer mosquitoes, limiting regional comparisons of abundance and community composition. CONCLUSIONS: This study represents the first systematic assessment of trapping methods in natural savanna ecosystems in southern Africa. We recommend the CDC trap or the net trap for future monitoring and surveillance programs.


Assuntos
Biodiversidade , Culicidae/classificação , Culicidae/crescimento & desenvolvimento , Entomologia/métodos , Densidade Demográfica , Animais , África do Sul , Análise Espacial
17.
Nat Commun ; 10(1): 4300, 2019 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-31541097

RESUMO

Mainstay treatment for Plasmodium vivax malaria has long relied on chloroquine (CQ) against blood-stage parasites plus primaquine against dormant liver-stage forms (hypnozoites), however drug resistance confronts this regimen and threatens malaria control programs. Understanding the basis of P. vivax chloroquine resistance (CQR) will inform drug discovery and malaria control. Here we investigate the genetics of P. vivax CQR by a cross of parasites differing in drug response. Gametocytogenesis, mosquito infection, and progeny production are performed with mixed parasite populations in nonhuman primates, as methods for P. vivax cloning and in vitro cultivation remain unavailable. Linkage mapping of progeny surviving >15 mg/kg CQ identifies a 76 kb region in chromosome 1 including pvcrt, an ortholog of the Plasmodium falciparum CQR transporter gene. Transcriptional analysis supports upregulated pvcrt expression as a mechanism of CQR.


Assuntos
Antimaláricos/farmacologia , Cloroquina/farmacologia , Cruzamentos Genéticos , Resistência a Medicamentos/genética , Proteínas de Membrana Transportadoras/genética , Plasmodium vivax/efeitos dos fármacos , Plasmodium vivax/genética , Proteínas de Protozoários/genética , Animais , Anopheles/parasitologia , Culicidae/parasitologia , Descoberta de Drogas , Feminino , Expressão Gênica , Genes de Protozoários , Malária/tratamento farmacológico , Malária Vivax/tratamento farmacológico , Malária Vivax/parasitologia , Masculino , Plasmodium falciparum/genética
18.
PLoS Negl Trop Dis ; 13(9): e0007700, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31509527

RESUMO

BACKGROUND: Rift Valley fever (RVF) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures. METHODOLOGY/PRINCIPAL FINDINGS: A first-line lateral flow immunochromatographic strip test (LFT) was developed for the detection of the nucleoprotein (N) of the RVF virus (RVFV). Its diagnostic performance characteristics were evaluated using reference stocks isolates recovered from different hosts and in geographic regions mimicking clinical specimens and from known RVF negative serum samples. A high level of diagnostic accuracy (DSe (35/35), DSp (167/169)) was observed, including the absence of cross-reactivity with viruses belonging to different genera. CONCLUSION/SIGNIFICANCE: The fact no specialized reagents and laboratory equipment are needed, make this assay a valuable, first-line diagnostic tool in resource-poor diagnostic territories for on-site RVFV detection, however the staff require training.


Assuntos
Imunoensaio/métodos , Febre do Vale de Rift/diagnóstico , Vírus da Febre do Vale do Rift/isolamento & purificação , Animais , Artiodáctilos/virologia , Culicidae/virologia , Nucleoproteínas/análise , Febre do Vale de Rift/sangue , Febre do Vale de Rift/virologia , Sensibilidade e Especificidade
19.
Cell Host Microbe ; 26(3): 309-313, 2019 09 11.
Artigo em Inglês | MEDLINE | ID: mdl-31513769

RESUMO

The inexorable emergence of mosquito-borne arboviruses and the failure of traditional vector control methods to prevent their transmission have triggered the development of alternative entomological interventions to render mosquito populations incapable of carrying arboviruses. Here, we use a theoretical framework to argue that decreasing mosquito tolerance to arbovirus infection could be a more evolutionarily sustainable disease control strategy than increasing mosquito resistance. Increasing resistance is predicted to select for mutant arboviruses escaping resistance, whereas reducing tolerance should lead to the death of infected vectors and thus select for mosquito-attenuated arbovirus variants that are less transmissible.


Assuntos
Infecções por Arbovirus/prevenção & controle , Arbovirus/fisiologia , Culicidae/virologia , Controle de Mosquitos/métodos , Mosquitos Vetores/virologia , Animais , Infecções por Arbovirus/transmissão , Evolução Biológica , Simulação por Computador , Interações Hospedeiro-Patógeno/fisiologia , Humanos , Modelos Biológicos
20.
J Photochem Photobiol B ; 199: 111620, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31522113

RESUMO

Current scenario of bio-nanotechnology, successfully fabrication of ultrafine titanium dioxide nanoparticles (TiO2NPs) using various biological protein sources for the multipurpose targets. The present research report involves synthesis of TiO2NPs using antimicrobial peptide (AMP) crustin (Cr). Crustin previously purified from the blue crab, Portunus pelagicus haemolymph, by blue Sepharose CL-6B matrix assisted affinity column chromatography. Synthesized Cr-TiO2NPs was physico-chemically characterized by UV-Visible spectroscopy (UV-Visible), X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), High-resolution transmission electron microscopy (HR-TEM) and zeta potential examination. X-ray diffraction analysis for crystalline nature and phase identification of titanium dioxide nanoparticles was absorbed. Functional groups were found through FTIR ranges between 1620 and 1700 cm-1. HR-TEM analysis showed that the synthesized Cr-TiO2NPs tetragonal shape and sizes ranging from 10 to 50 nm. Finally, the surface charge of the Cr-TiO2NPs was confirmed through zeta potential analysis. Furthermore, the characterized Cr-TiO2NPs exhibited good biofilm inhibition against GPB - S. mutans (Gram Positive Bacteria- Streptococcus mutans), GNB - P. vulgaris (Gram Negative Bacteria- Proteus vulgaris) and fungal Candida albicans. Moreover, photocatalysis demonstrated that the Cr-TiO2NPs was effectively explored the degradation of dyes. The results suggest that Cr-TiO2NPs is an excellent bactericidal, fungicidal and photocatalytic agent that can be supportively used for biomedical and industrial applications.


Assuntos
Anti-Infecciosos/química , Peptídeos Catiônicos Antimicrobianos/química , Inseticidas/química , Nanocápsulas/química , Processos Fotoquímicos , Titânio/química , Animais , Anti-Infecciosos/farmacologia , Peptídeos Catiônicos Antimicrobianos/farmacologia , Biofilmes , Braquiúros/química , Candida albicans/efeitos dos fármacos , Catálise , Sobrevivência Celular/efeitos dos fármacos , Culicidae , Liberação Controlada de Fármacos , Humanos , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Luz , Estrutura Molecular , Proteus vulgaris/efeitos dos fármacos , Streptococcus mutans/efeitos dos fármacos
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