Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 13.931
Filtrar
1.
J Ethnopharmacol ; 300: 115671, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36055476

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Kalyanaka ghrita (KG) is an Ayurvedic formulation traditionally used in the treatment of Daurbalya (debility) and Smritidaurbalya (impairment of intellectual activities). Clinical studies have reported the effect of KG in the treatment of Manasmandata or Buddhimandyata which is associated with impaired learning, social adjustment and maturation. AIM OF THE STUDY: The present study aims to standardization of KG and validation of its use in experimental models of neurodegeneration. MATERIALS AND METHODS: KG was Standardized for biomarkers curcumin, gallic acid, tannic acid, chebulagic acid, and berberine. In male wistar rats, neurodegeneration was induced by administration of intracerebroventricular Amyloid ß (Aß1-42). The effect of KG (oral and intranasal treatment) was evaluated through behavioral parameters such as Morris water maze, social recognition test, novel object recognition, locomotor activity, and molecular parameters, brain acetylcholinesterase, brain-derived neurotrophic factor (BDNF), inflammatory cytokines, oxidative stress markers, and antioxidants. Brain histopathology was performed for studying the architecture of the brain and plaque formation. RESULTS AND DISCUSSION: A novel HPLC method has been developed for the standardization of KG. Treatment with KG significantly improved cognition and memory and increased brain BDNF and antioxidant status in Aß1-42 induced rats. It also reduced brain acetylcholinesterase, oxidative stress, and inflammatory cytokines and prevented neuronal damage. There were more marked effects with intra-nasal administration compared to oral treatment. CONCLUSION: The findings suggest that KG has neuroprotective potential and along with its nootropic property could be a promising therapy for neurodegenerative diseases like Alzheimer's disease.


Assuntos
Doença de Alzheimer , Berberina , Curcumina , Fármacos Neuroprotetores , Nootrópicos , Acetilcolinesterase , Doença de Alzheimer/tratamento farmacológico , Peptídeos beta-Amiloides/toxicidade , Animais , Antioxidantes , Berberina/farmacologia , Fator Neurotrófico Derivado do Encéfalo , Curcumina/farmacologia , Citocinas/farmacologia , Modelos Animais de Doenças , Masculino , Aprendizagem em Labirinto , Transtornos da Memória/induzido quimicamente , Transtornos da Memória/tratamento farmacológico , Nootrópicos/farmacologia , Ratos , Ratos Wistar , Taninos/farmacologia
2.
Food Chem ; 399: 133952, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35998492

RESUMO

Although excessive pharmaceutical activities of curcumin have been reported, the poor solubility, low stability and low bioavailability greatly limited its application. In this study, the interaction between protein Z (PZ) and curcumin, and the effects of PZ on the stability and bioavailability of curcumin were investigated. Fluorescence quenching results indicated that curcumin molecule binds PZ with a stoichiometry of 4:1, and the binding affinity is stronger than other reported protein carriers. Molecular dynamics simulation results suggested that curcumin binds in the hydrophobic region of PZ, and the interaction was maintained mainly by hydrogen-bond (Pro-287, Asn-340 and Tyr-234). PZ-curcumin complex possessed better encapsulation efficiency (64.10 %) and loading capacity (5.49 µg/mg) for curcumin. In addition, binding with PZ not only improved the thermal, light and digestive stability of curcumin significantly, but lowered its toxic effect on Caco-2 cells and improved relative bioavailability (305 %) compared with that of curcumin only.


Assuntos
Curcumina , Hordeum , Nanopartículas , Disponibilidade Biológica , Proteínas Sanguíneas , Células CACO-2 , Curcumina/química , Portadores de Fármacos/química , Hordeum/metabolismo , Humanos , Nanopartículas/química , Solubilidade
3.
Food Chem ; 400: 134006, 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36058041

RESUMO

The interaction between high-density lipoprotein hydrolysate hydrolyzed by trypsin (EHT) and carboxymethyl dextrin (CMD) at pH 7.0, and in vitro digestibility of high internal phase emulsions (HIPEs) were studied. The particle size of EHT/CMD mixtures increased from 207.2 nm to 1229.6 nm with increase of the ratio of EHT to CMD from 8:1 to 1:2. The proportion of EHT adsorbed on the surface increased from 60.83 % to 80.81 %, forming thicker interfacial layer, exhibiting higher resistance to centrifugation and ionic strength. And more uniform proton density distribution and shorter relaxation time (T2) were charactered in HIPEs formed by mixtures. Rheological analysis further proved that CMD could lead to high viscosity, high elasticity, excellent oscillation performance of HIPEs. The spatial and electrostatic repulsion forces reduced the aggregation of oil droplets and promoted the formation of more mixed micelles, improving the bioaccessibility of curcumin. These findings provided theoretical strategies for lipid-soluble nutrients delivery systems.


Assuntos
Curcumina , Adsorção , Dextrinas , Emulsões , Lipoproteínas HDL , Micelas , Tamanho da Partícula , Prótons , Tripsina
4.
Food Chem ; 402: 134476, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36303367

RESUMO

Triple-layered, pH-sensitive and active films based on furcellaran (FUR), chitosan (CHIT) and gelatin hydrolysates (HGEL) were obtained for the first time. Curcumin ethanol extract (CUR) enriched with lemongrass essential oil (LEO) in three concentrations was added to the middle layer of the film. SEM and AFM analysis confirmed the formation of 3 layers, and CUR-LEO was evenly dispersed in the 2nd layer. The addition of CUR-LEO influenced the color of the surface and improved the UV blocking properties of the film. However, the mechanical and water vapor barrier properties remained unchanged. The CUR-LEO demonstrated antibacterial properties against bacteria and fungi. The antioxidant activity of the films increased along with the increasing concentrations of CUR-LEO. The presented films were able to effectively extend the storage life of tomatoes. Moreover, the colored films had the monitoring freshness effect with a distinct color change during carp storage.


Assuntos
Quitosana , Curcumina , Cymbopogon , Óleos Voláteis , Gelatina , Curcumina/farmacologia , Embalagem de Alimentos , Óleos Voláteis/farmacologia , Antioxidantes/farmacologia , Extratos Vegetais/farmacologia
5.
Food Chem ; 402: 134328, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36303375

RESUMO

Glycolipids may be potential materials to improve the instability of liposomes during storage and consumption. Curcumin-loaded liposomes with high stability were successfully prepared by glycolipids and phospholipids extracted from tilapia. The physicochemical properties analysed showed that glycolipids enhanced the surface charge of liposomes and the encapsulation ability of curcumin. The enhanced affinity of liposomes for curcumin was attributed to the stronger interaction between the head group of glycolipids and curcumin through hydrogen bonding. As predicted, glycolipids improved the storage stability of liposomes, and the thermal stability of curcumin increased from 35.95% to 54.13%. Moreover, glycolipids could resist the degradation of liposomes in the gastrointestinal tract, reducing the encapsulation efficiency changes of curcumin from 60.67% to 43.63%. Simultaneously, the liposomes formed by glycolipids could more effectively protect nerve cells from oxidative damage. Therefore, the substitution of phospholipids with glycolipids is an effective strategy to improve the stability and bioactivity of liposomes.


Assuntos
Curcumina , Lipossomos , Lipossomos/química , Fosfolipídeos/química , Glicolipídeos/química , Curcumina/química , Estabilidade de Medicamentos
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121806, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36108405

RESUMO

Curcumin (bis-α,ß-unsaturated ß-diketone) plays an important role in the prevention of numerous diseases, including diabetes. Curcumin, as an enzyme inhibitor, has ideal structural properties including hydrophobic nature, flexible backbone, and several available hydrogen bond (H-bond) donors and acceptors. In this study, curcumin-fused aldohexose derivatives 3(a-c) were synthesized and used as influential agents in the treatment of diabetes with inhibitory properties against two carbohydrate-hydrolyzing enzymes α-glucosidase (α-Gls) and α-amylase (α-Amy) which are known to be significant therapeutic targets for the reduction of postprandial hyperglycemia. These compounds were isolated, purified, and then spectrally characterized via FT-IR, Mass, 1H, and 13C NMR, which strongly confirmed the targeted product's formation. Also, their inhibitory properties against α-Gls and α-Amy were evaluated spectroscopically. The Results indicated that all compounds strongly inhibited α-Amy and α-Gls by mixed and competitive mechanisms, respectively. The intrinsic fluorescence of α-Amy was quenched by the interaction with compounds 1 and 3b through a dynamic quenching mechanism, and the 1 and 3b/α-Amy complexes were spontaneously formed, mainly driven by the hydrophobic interaction and hydrogen bonding. Fourier transform infrared spectra (FT-IR) comprehensively verified that the binding of compounds 1 and 3b to α-Amy would change the conformation and microenvironment of α-Amy, thereby inhibiting the enzyme activity. Docking and molecular dynamics (MD) simulations showed that all compounds interacted with amino acid residues located in the active pocket site of the proteins. In vivo studies confirmed the plasma glucose diminution after the administration of compound 3b to Wistar rats. Accordingly, the results of the current work may prompt the scientific communities to investigate the possibility of compound 3b application in the clinic.


Assuntos
Curcumina , Diabetes Mellitus , Ratos , Animais , Hipoglicemiantes/química , Curcumina/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Ratos Wistar , alfa-Glucosidases/metabolismo , alfa-Amilases/metabolismo , Simulação de Acoplamento Molecular , Inibidores de Glicosídeo Hidrolases/química
7.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121916, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36201868

RESUMO

Alternative therapies against pathogens are under intense investigation because of their increasing resistance to antibiotics. Photodynamic therapy (PDT) is one such alternative that has shown promising results. However, for the widespread use of PDT, it is essential to decipher underlying mechanisms, so as to improve PDT's therapeutic applications. Because of this, we have studied biochemical changes in pathogen Pseudomonas aeruginosa, a medically important bacteria that has developed antibiotic resistance, after PDT with curcumin photosensitizer. Results show a drastic decrease in α-helix protein and increased disordered and ß-sheet secondary structure proteins in P. Aeruginosa post-PDT compared to control. Interestingly, these biochemical changes differ from PDT of pathogens Leishmania braziliensis and Leishmania major with photosensitizer methylene blue. This observation underlines the need for extensive studies on PDT of different pathogens to understand mechanisms of action and develop better PDT strategies.


Assuntos
Curcumina , Fotoquimioterapia , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/farmacologia , Fármacos Fotossensibilizantes/uso terapêutico , Pseudomonas aeruginosa , Curcumina/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier
8.
J Sci Food Agric ; 103(1): 135-142, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35833377

RESUMO

BACKGROUND: Oleogels represent one of the most important carriers for the delivery of lipophilic nutraceuticals. Phytosterols (PS), plant-derived natural sterol compounds, are preferred for oleogel preparation due to their self-assembly properties and health function. However, the relationship between the physical properties of PS-based oleogels and the chemical stability of loaded bioactive compounds is still unclear. RESULTS: The influence of lecithin (LC) and glycerol monostearate (GMS) on the physical properties of PS-based oleogels made of liquid coconut oil and the stability of curcumin as a model bioactive loaded in the oleogels was investigated. Results showed that the flow consistency index was much higher for GMS-containing oleogels than that for LC-containing oleogels. The optical microscopy and X-ray scattering analysis showed that the addition of GMS in the PS oleogels promoted the formation of a crystal mixture with different crystal polymorph structures, whereas LC addition promoted the formation of needle-like crystals of PS. Using curcumin as a model lipophilic nutraceutical, the GMS-enriched PS oleogels with high crystallinity and flow consistency index exhibited a good retention ratio and scavenging activity of the loaded curcumin when stored at room temperature. CONCLUSION: This study shows that enhancing the firmness of oleogels made from PS and liquid coconut oil is beneficial to the retention and chemical stability of a loaded bioactive (curcumin). The findings of the study will boost the development of PS-based oleogel formulations for lipophilic nutraceutical delivery. © 2022 Society of Chemical Industry.


Assuntos
Curcumina , Fitosteróis , Fitosteróis/química , Tensoativos , Óleo de Coco , Excipientes
9.
Chemosphere ; 310: 136910, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36270524

RESUMO

Reverse osmosis (RO) membrane has been widely used in various water treatment fields as an efficient desalination technology, but serious biofouling problem arises in the actual application process. Curcumin is known as a natural compound that can reduce biofouling by inhibiting the growth of microorganisms based on quorum sensing. Dopamine, a molecule with excellent adhesion and functionalization on the material's surface, has high research value for applying a curcumin coating to the membrane surface. Curcumin degrades under alkaline conditions, whereas dopamine must polymerize under alkaline conditions. Simultaneously, a coating may adversely affect curcumin. Therefore, a two-step coating process was considered by self-polymerizing dopamine on the thin-film composite membrane surface and then dip-coating curcumin attached to the polydopamine layer. Furthermore, the effect of time and concentration on the surface modification before and after membrane modification was investigated. The highest permeability of 1.39 L/m2/hr/bar was achieved with the modified membranes. The number of gram-positive bacteria decreased from 6.71 × 106 to 9.67 × 105 CFU/mL. This result is meaningful for antifouling through modification of the membrane surface. Use of curcumin can be applied to reduce biofouling and extend the lifetime of the membrane without pretreatment or membrane cleaning.


Assuntos
Incrustação Biológica , Curcumina , Purificação da Água , Incrustação Biológica/prevenção & controle , Curcumina/farmacologia , Osmose , Membranas Artificiais , Dopamina
10.
Food Chem ; 402: 134262, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36126584

RESUMO

In this work, double-layered emulsions stabilized by bacterial cellulose nanofibers/soy protein isolate/chitosan complex were prepared using layer-by-layer self-assembly technology and used for the delivery of curcumin, and the physical and delivery properties of the prepared double-layered emulsions were investigated. The zeta-(ζ) potential of colloidal particles confirmed that the chitosan was adsorbed on surface of the single-layered emulsion droplets by attractive electrostatic interactions. Meanwhile, the physical properties showed that the double-layered emulsions displayed high pH-sensitivity and showed relatively higher stability under acid environment. Furthermore, the in vitro digestion result showed that the curcumin loaded by double-layered emulsions were more stable during simulated gastric digestion as compared with that loaded by single-layered emulsions. Especially, the bioaccessibility of curcumin delivered by the double-layered emulsions (77.4 ± 3.25%) was significantly higher than that of curcumin delivered by single-layered emulsions (32.8 ± 2.56%). Therefore, it can be concluded that the prepared double-layered emulsions showed a great potential application in the delivery of fat-soluble bioactive compounds.


Assuntos
Quitosana , Curcumina , Nanofibras , Emulsões/química , Curcumina/química , Celulose/química , Proteínas de Soja , Digestão , Bactérias/metabolismo , Concentração de Íons de Hidrogênio , Tamanho da Partícula
11.
Food Chem ; 402: 134179, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36137387

RESUMO

Pickering emulsion gels (PKEGs) are being explored as solid fat substitutes and delivery systems due to their semi-solid textures and high stabilities. However, these PKEGs have relatively high-fat content, which is undesirable for nutritional and cost reasons. Therefore, in this study, low-fat PKEGs (10 % oil content) were successfully fabricated using zein/phytic acid (ZPA) complex nanoparticles with zein to phytic acid mass ratio of 1:0.006. These nanoparticles have a mean diameter of around 161 nm and wettability of around 89°. The formation of PKEGs were confirmed by the results of dynamic rheology (G' > G″). Confocal laser scanning microscope showed that the complex nanoparticles formed a dense barrier on the surface of the oil droplets, which prevented the oil droplets against coalescence. The chemical stability of curcumin was greatly improved by encapsulation in the PKEGs. The low-fat PKEGs developed in this study may be effective delivery systems for hydrophobic bioactive substances.


Assuntos
Curcumina , Substitutos da Gordura , Nanopartículas , Zeína , Zeína/química , Emulsões/química , Ácido Fítico , Curcumina/química , Tamanho da Partícula , Géis/química , Nanopartículas/química
12.
Food Chem ; 403: 134388, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36183471

RESUMO

A new protein complex (SAKP-Cur) was successfully prepared by combining soluble Antarctic krill protein with curcumin through hydrophobic action. The potency of photodynamic inactivation (PDI) mediated by the complex on preserving the storage quality of shrimp at 4 °C was investigated by microbiological, chemical, physical and histological methods. Results showed that the SAKP-Cur significantly improved the stability of curcumin, and greatly inactivated the native bacteria in shrimp driven by PDI. Meanwhile, the complex-mediated PDI effectively reduced the endogenous enzyme activity, the production of total volatile basic nitrogen (TVB-N) and malondialdehyde (MDA) in shrimp. Moreover, it obviously maintained the integrity and elasticity of the muscle fibers, thereby reducing the loss of water in myofibrils. Notably, the SAKP-Cur enhanced the PDI potency to preserve the freshness of shrimp during 4 °C storage or freeze-thaw cycles treatment. Therefore, the SAKP-Cur coupled with PDI is an effective fresh-keeping technology for aquatic products.


Assuntos
Curcumina , Euphausiacea , Penaeidae , Animais , Curcumina/farmacologia , Frutos do Mar/análise , Alimentos Marinhos , Penaeidae/química
13.
Food Chem ; 403: 134344, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36183473

RESUMO

In this study, zein/hydroxypropyl-beta-cyclodextrin nanoparticles (ZHNPs) were synthesized using a combination of antisolvent co-precipitation and electrostatic attraction. The structural and physicochemical properties of the NPs were characterized using a variety of analytical methods. NPs with small mean diameters (143.6 nm) and strong cationic surface potentials (+62.1 mV) could be obtained at a mass ratio of zein-to-HP-ß-CD of 1:1. These NPs were then used to encapsulate a model hydrophobic nutraceutical, curcumin. The results of zeta-potential, Fourier-transform infrared spectroscopy, X-ray diffractometry, and fluorescence spectroscopy measurements suggested that electrostatic, hydrogen bonding, and hydrophobic interactions were the main driving forces for NPs formation and curcumin encapsulation. The NPs had a relatively high encapsulation efficiency (89.41 %) for curcumin and improved its antioxidant activity (3.6-fold that of free curcumin) and photostability. Consequently, they could be used as effective food-grade delivery systems for low water-soluble bioactive substances.


Assuntos
Curcumina , Nanopartículas , Zeína , Zeína/química , Curcumina/química , 2-Hidroxipropil-beta-Ciclodextrina , Eletricidade Estática , Nanopartículas/química , Interações Hidrofóbicas e Hidrofílicas , Tamanho da Partícula
14.
J Mol Neurosci ; 72(3): 653-676, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34697770

RESUMO

Neurodegenerative diseases (NDs) such as Alzheimer's disease, Parkinson's disease, Huntington disease, amyotrophic lateral sclerosis, and prion disease affect any part of the brain. The complete mechanism of ND is unknown, but there are some molecular mechanism and chemical process. Natural compounds have better compatibility with the human body along with lesser side effects. Moreover, several studies showed that various natural compounds have significant neuroprotective, potent antioxidant, and anti-inflammatory properties, which are effective for treating the different type of ND. In ND, natural compounds act by various mechanisms such as preventing the generation of reactive oxygen species (ROS), eliminating destructed biomolecules before their accumulation affects cell metabolism, and improving the disease conditions. But due to the presence of the blood-brain barrier (BBB) layer and unfavorable pharmacokinetic properties of natural compounds, their delivery into the brain is limited. To minimize this problem and enhance drug delivery into the brain with an effective therapeutic dose, there is a need to develop a practical novel approach. The various studies showed that nanoformulations and microneedles (MN) containing natural compounds such as quercetin, curcumin, resveratrol, chrysin, piperine, ferulic acid, huperzine A, berberine, baicalein, hesperetin, and retinoic acid effectively improved many ND. In this review, the effect of such natural drug-loaded nanoformulation and MN patches on ND management is discussed, along with their merits and demerits. This review aims to introduce different novel approaches for enhancing natural drug delivery into the brain to manage various neurodegenerative diseases.


Assuntos
Produtos Biológicos , Curcumina , Doenças Neurodegenerativas , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Produtos Biológicos/farmacologia , Produtos Biológicos/uso terapêutico , Curcumina/uso terapêutico , Sistemas de Liberação de Medicamentos , Humanos , Doenças Neurodegenerativas/tratamento farmacológico , Doenças Neurodegenerativas/metabolismo
15.
Sci Rep ; 12(1): 18691, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36333357

RESUMO

Oral biofilms are directly linked to one of the most common chronic human diseases, dental caries. Resin-based dental materials have significant potential to replace amalgam, however they lack sufficient antimicrobial power. This innovative study investigates a curcumin-loaded dental resin which can be utilized in an antimicrobial photodynamic therapy (aPDT) approach. The study evaluated the effects of curcumin loading on resin physicochemical, mechanical, and adhesive properties, as well as the antimicrobial response associated with blue light activation. Preliminary tests involving degree of conversion (DC) and sample integrity determined the optimal loading of curcumin to be restricted to 0.05 and 0.10 wt%. These optimal loadings were tested for flexural strength (FS), water sorption (WS) and solubility (SL), shear bond strength to dentin (SBS), and viability of Streptococcus mutans under 14.6 J/cm2 blue light or dark conditions, in 6 h and 24 h biofilms. The results demonstrated that 0.10 wt% curcumin had minimal impact on either FS or SBS, but detectably increased WS and SL. A 2 log10 (CFU/mL) reduction in S. mutans after light application in both 6 h and 24 h biofilms were corroborated by CLSM imaging and highlighted the significant potential of this novel aPDT approach with resin-based dental materials.


Assuntos
Anti-Infecciosos , Curcumina , Cárie Dentária , Fotoquimioterapia , Humanos , Metacrilatos/química , Curcumina/farmacologia , Cárie Dentária/tratamento farmacológico , Streptococcus mutans , Fotoquimioterapia/métodos , Anti-Infecciosos/farmacologia , Antibacterianos/farmacologia , Resinas Sintéticas/química , Materiais Dentários/farmacologia , Teste de Materiais
16.
Allergy Asthma Proc ; 43(6): 543-545, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36335415

RESUMO

Background: Curcumin has been shown to decrease allergic symptoms and biomarkers in some animal and human studies. Objective: Our study aimed to determine if curcumin affects immediate skin-prick testing. Methods: We enrolled 34 participants sensitized to select antigens. The participants were randomized to treatment with curcumin or placebo in a double-blind fashion. The participants underwent titrated skin-prick testing before and after 1 week of treatment, and the pre- and posttreatment skin test wheals and flares were compared. Results: Curcumin did not have a statistically significant effect on immediate skin-prick test wheal or flare size. Conclusion: Although curcumin may attenuate allergic symptoms and biomarkers, it does not have a significant effect on immediate skin-prick test results and does not need to be discontinued before testing.


Assuntos
Curcumina , Urticária , Animais , Humanos , Curcumina/farmacologia , Estudos Cross-Over , Testes Cutâneos/métodos , Método Duplo-Cego , Histamina , Pele
17.
Sci Rep ; 12(1): 18403, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36319750

RESUMO

Liver fibrosis results from the hepatic accumulation of the extracellular matrix accompanied by a failure of the mechanisms responsible for matrix dissolution. Pathogenesis of liver fibrosis is associated with many proteins from different cell types. In the present study, in silico molecular docking analysis revealed that curcumin may inhibit the fibrosis-mediating proteins PDGF, PDGFRB, TIMP-1, and TLR-9 by direct binding. Nano-formulation can overcome curcumin problems, increasing the efficacy of curcumin as a drug by maximizing its solubility and bioavailability, enhancing its membrane permeability, and improving its pharmacokinetics, pharmacodynamics and biodistribution. Therefore, green silver nanoparticles (AgNPs) were synthesized in the presence of sunlight by means of the metabolite of Streptomyces malachiticus, and coated with curcumin-chitosan mixture to serve as a drug delivery tool for curcumin to target CCl4-induced liver fibrosis mouse model. Fibrosis induction significantly increased hepatic gene expression of COL1A1, α-SMA, PDGFRB, and TIMP1, elevated hepatic enzymes, increased histopathological findings, and increased collagen deposition as determined by Mason's trichrome staining. Treatment with naked AgNPs tended to increase these inflammatory effects, while their coating with chitosan, similar to treatment with curcumin only, did not prevent the fibrogenic effect of CCl4. The induction of liver fibrosis was reversed by concurrent treatment with curcumin/chitosan-coated AgNPs. In this nano form, curcumin was found to be efficient as anti-liver fibrosis drug, maintaining the hepatic architecture and function during fibrosis development. This efficacy can be attributed to its inhibitory role through a direct binding to fibrosis-mediating proteins such as PDGFRB, TIMP-1, TLR-9 and TGF-ß.


Assuntos
Quitosana , Curcumina , Nanopartículas Metálicas , Nanopartículas , Camundongos , Animais , Curcumina/farmacologia , Quitosana/química , Prata/farmacologia , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Nanopartículas Metálicas/química , Distribuição Tecidual , Receptor beta de Fator de Crescimento Derivado de Plaquetas/metabolismo , Simulação de Acoplamento Molecular , Receptor Toll-Like 9/metabolismo , Cirrose Hepática/metabolismo , Nanopartículas/química
18.
Nutrients ; 14(21)2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36364884

RESUMO

Menopause is marked by a gradual and permanent decrease of estrogen from the ovaries, leading to metabolic and physiological changes in the body. Combined with increased body mass index, postmenopausal women have elevated systemic inflammation and metabolic disturbances leading to increased risk of developing chronic diseases. A bioactive coconut yoghurt containing curcumin and chlorogenic acid was developed with the potential to target inflammatory processes. In this randomized crossover study, healthy postmenopausal women with a BMI of 25-40 were recruited to consume 125 g of either the bioactive or placebo yoghurt. Blood samples were collected at baseline, 30 min, and 1, 2, 3 and 4 h postprandially. Plasma inflammatory markers (TNFα and IL6) and metabolic markers (triglycerides, insulin and glucose) were measured. Participants had significantly lower plasma TNFα Cmax after consumption of the bioactive yoghurt compared to placebo (mean difference = 0.3 pg/mL; p = 0.04). Additionally, plasma TNFα was significantly lower postprandially compared to baseline after consumption of the bioactive yogurt but not the placebo. No differences were observed in the metabolic markers measured. Conclusions: The bioactive yoghurt fortified with curcumin and chlorogenic acid has the potential to reduce inflammatory mediators; however, a larger and longer-term study is required to confirm these findings.


Assuntos
Curcumina , Iogurte , Humanos , Feminino , Fator de Necrose Tumoral alfa , Ácido Clorogênico , Pós-Menopausa , Estudos Cross-Over , Inflamação/prevenção & controle
19.
ACS Appl Bio Mater ; 5(11): 5418-5431, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36326507

RESUMO

Nanoparticle drug delivery systems have drawn considerable attention worldwide due to their unique characteristics and advantages in anticancer drug delivery. Herein, the curcumin (Cur) loaded nanomicelles with two-stage drug release behavior were developed. ß-Cyclodextrin (ß-CD) and cholesterol were conjugated onto both ends of the poly(ethylene glycol) (PEG) chain to obtain an amphiphilic ß-CD-PEG-Chol. The Cur was loaded into the cavities of ß-CD and nanomicelle when the ß-CD-PEG-Chol self-assembled to the Cur@ß-CD-PEG-Chol nanomicelles (Cur@CPC NMs). These Cur@CPC NMs are spherical particles with a particle size of 120.9 nm. The Cur drug loading capacity of Cur@CPC NMs are 61.6 ± 6.9 mg/g. The release behavior of Cur from Cur@CPC NMs conformed to a two-stage mode of "burst-release followed by sustained-release". The prepared Cur@CPC NMs possess high storage stability and excellent hemocompatibility. Moreover, these Cur@CPC NMs exhibit satisfactory antioxidant activity and anticancer activity, resulting in significant reduction in intracellular H2O2-induced ROS and a nearly 50% lethality rate of HepG-2 cells. Meanwhile, the Cur@CPC NMs show good anti-inflammatory activity, by which the secretion of inflammatory factors of IL-6 and TNF-α are inhibited. Overall, the developed Cur@CPC NMs show application prospects in anticancer drug delivery systems.


Assuntos
Antineoplásicos , Curcumina , beta-Ciclodextrinas , Peróxido de Hidrogênio , Polietilenoglicóis , Sistemas de Liberação de Medicamentos , Colesterol , Curcumina/farmacologia , Antineoplásicos/farmacologia
20.
Front Endocrinol (Lausanne) ; 13: 1051111, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36387924

RESUMO

Background: Polycystic ovary syndrome (PCOS) is a multi-factorial heterogeneous syndrome that has both adverse reproductive and metabolic implications for affected women and its management is a challenging clinical problem. Curcumin, as a phenolic compound with potent anti-inflammatory and antioxidant properties exerting positive effects on the lipid profile and insulin resistance, appears to be a valuable treatment regimen for patients with PCOS. Objective: This study aimed to evaluate the efficacy and safety of curcumin in the treatment of PCOS. Methods: Chinese databases (Chinese National Knowledge Infrastructure, China Biology Medicine Databases, VIP database, Wanfang Database, and Chinese Clinical Trial Registry) and English databases (PubMed, Web of Science, Embase, Cochrane Library, Scopus and Clinical trials) were thoroughly investigated through screening randomized controlled trials on curcumin in PCOS published from the date of inception to May 2022. Standardized data search and abstraction were conducted following the preferred reporting items for systematic reviews and meta-analysis (PRISMA) statement. Quantitative and qualitative analyses were performed. Heterogeneity was assessed using I2 statistics. Results: A total of 447 patients from seven randomized controlled trials were included in the meta-analysis. Results showed that the ingestion of curcumin decreased body mass index (WMD -0.267, 95% CI -0.450 to -0.084, P = 0.004, I2 = 0.0%), fasting plasma glucose (WMD -3.618, 95% CI -5.165 to -2.071, P < 0.001, I2 = 20.4%), insulin (WMD -1.834, 95% CI -2.701 to -0.968, P < 0.001, I2 = 8.4%), homeostatic model assessment for insulin resistance (WMD -0.565, 95% CI -0.779 to -0.351, P < 0.001, I2 = 0.0%), total cholesterol (WMD -15.591, 95% CI -27.908 to -3.273, P = 0.013, I2 = 68.9%), C-reactive protein (WMD -0.785, 95% CI -1.553 to -0.017, P = 0.045, I2 = 23.9%), and increased the quantitative insulin sensitivity check index (WMD 0.011, 95% CI 0.005 to 0.017, P = 0.001, I2 = 39.6%). As for safety, the treatment group did not cause significant adverse reactions than that in the control group. Conclusion: In light of presented findings, curcumin has beneficial effects on serum markers of inflammation, weight loss and glucose and lipid metabolism in patients with PCOS. The incidence of adverse reactions does not increase with the application of curcumin. However, a larger, more definitive study is needed to further investigate these results. Systematic review registration: https://www.crd.york.ac.uk/prospero/, identifier CRD42022332394.


Assuntos
Curcumina , Resistência à Insulina , Síndrome do Ovário Policístico , Humanos , Feminino , Síndrome do Ovário Policístico/tratamento farmacológico , Síndrome do Ovário Policístico/metabolismo , Curcumina/uso terapêutico , Insulina/uso terapêutico , Proteína C-Reativa
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...