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1.
J Cell Biol ; 222(1)2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36239631

RESUMO

At the trans-Golgi, complex traffic connections exist to the endolysosomal system additional to the main Golgi-to-plasma membrane secretory route. Here, we investigated three hits in a Drosophila screen displaying secretory cargo accumulation in autophagic vesicles: ESCRT-III component Vps20, SNARE-binding Rop, and lysosomal pump subunit VhaPPA1-1. We found that Vps20, Rop, and lysosomal markers localize near the trans-Golgi. Furthermore, we document that the vicinity of the trans-Golgi is the main cellular location for lysosomes and that early, late, and recycling endosomes associate as well with a trans-Golgi-associated degradative compartment where basal microautophagy of secretory cargo and other materials occurs. Disruption of this compartment causes cargo accumulation in our hits, including Munc18 homolog Rop, required with Syx1 and Syx4 for Rab11-mediated endosomal recycling. Finally, besides basal microautophagy, we show that the trans-Golgi-associated degradative compartment contributes to the growth of autophagic vesicles in developmental and starvation-induced macroautophagy. Our results argue that the fly trans-Golgi is the gravitational center of the whole endomembrane system.


Assuntos
Autofagia , Endossomos , Complexo de Golgi , Lisossomos , Animais , Drosophila , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Complexos Endossomais de Distribuição Requeridos para Transporte/genética , Complexos Endossomais de Distribuição Requeridos para Transporte/metabolismo , Endossomos/metabolismo , Complexo de Golgi/metabolismo , Lisossomos/metabolismo , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Transporte Proteico , Proteínas SNARE/genética , Proteínas SNARE/metabolismo , Proteínas rab de Ligação ao GTP
2.
J Cell Biol ; 222(1)2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36239632

RESUMO

Membrane trafficking is essential for sculpting neuronal morphology. The GARP and EARP complexes are conserved tethers that regulate vesicle trafficking in the secretory and endolysosomal pathways, respectively. Both complexes contain the Vps51, Vps52, and Vps53 proteins, and a complex-specific protein: Vps54 in GARP and Vps50 in EARP. In Drosophila, we find that both complexes are required for dendrite morphogenesis during developmental remodeling of multidendritic class IV da (c4da) neurons. Having found that sterol accumulates at the trans-Golgi network (TGN) in Vps54KO/KO neurons, we investigated genes that regulate sterols and related lipids at the TGN. Overexpression of oxysterol binding protein (Osbp) or knockdown of the PI4K four wheel drive (fwd) exacerbates the Vps54KO/KO phenotype, whereas eliminating one allele of Osbp rescues it, suggesting that excess sterol accumulation at the TGN is, in part, responsible for inhibiting dendrite regrowth. These findings distinguish the GARP and EARP complexes in neurodevelopment and implicate vesicle trafficking and lipid transfer pathways in dendrite morphogenesis.


Assuntos
Dendritos , Complexos Multiproteicos , Proteínas de Transporte Vesicular , Rede trans-Golgi , Animais , Proteínas de Transporte , Dendritos/metabolismo , Drosophila , Proteínas de Drosophila , Complexo de Golgi/metabolismo , Complexos Multiproteicos/metabolismo , Receptores de Esteroides , Esteróis/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Rede trans-Golgi/metabolismo
3.
J Hazard Mater ; 441: 129826, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36084456

RESUMO

Metastasis includes tumor invasion and migration and underlies over 90% of cancer mortality. The metastatic effects of environmental carcinogens raised serious health concerns. However, the underlying mechanisms remained poorly studied. In the present study, an in vivo RasV12/lgl-/- model of the fruitfly, Drosophila melanogaster, with an 8-day exposure was employed to explore the metastatic effects of 3,3',4,4',5-pentachlorobiphenyl (PCB126), perfluorooctanoic acid (PFOA) and cadmium chloride (CdCl2). At 1.0 mg/L, PCB126, PFOA, and CdCl2 significantly increased tumor invasion rates by 1.32-, 1.33-, and 1.29-fold of the control, respectively. They also decreased the larval body weight and locomotion behavior. Moreover, they commonly disturbed the expression levels of target genes in MAPK and UPR pathways, and their metastatic effects were significantly abolished by the addition of p38 inhibitor (SB203580), JNK inhibitor (SP600125) and IRE1 inhibitor (KIRA6). Notably, the addition of the IRE inhibitor significantly influenced sna/E-cad pathway which is essential in both p38 and JNK regulations. The results demonstrated an essential role of sna/E-cad in connecting the effects of carcinogens on UPR and MAPK regulations and the resultant metastasis.


Assuntos
Carcinógenos Ambientais , Neoplasias , Animais , Cloreto de Cádmio , Caprilatos , Drosophila , Drosophila melanogaster , Fluorcarbonetos , Proteínas Serina-Treonina Quinases , Transdução de Sinais
4.
Semin Cell Dev Biol ; 133: 74-82, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35365398

RESUMO

Cells with subcellular lumens form some of the most miniature tubes in the tubular organs of animals. These are often crucial components of the system, executing functions at remote body locations. Unlike tubes formed by intercellular or autocellular junctions, the cells with junctionless subcellular lumens face unique challenges in modifying the cell shape and plasma membrane organization to incorporate a membrane-bound tube within, often associated with dramatic cellular growth and extensions. Results in the recent years have shown that membrane dynamics, including both the primary delivery and recycling, is crucial in providing the cell with the flexibility to face these challenges. A significant portion of this information has come from two in vivo invertebrate models; the Drosophila tracheal terminal cells and the C. elegans excretory cell. This review focuses on the data obtained from these systems in the recent past about how trafficking pathways influence subcellular tube and branching morphogenesis. Given that such tubes occur in vertebrate vasculature, these insights are relevant to human health, and we contrast our conclusions with the less understood subcellular tubes of angiogenesis.


Assuntos
Proteínas de Drosophila , Animais , Humanos , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Caenorhabditis elegans/metabolismo , Morfogênese , Drosophila/metabolismo
5.
Artigo em Inglês | MEDLINE | ID: mdl-36306997

RESUMO

The resting membrane potential of most cells is maintained by potassium K2p channels. The pharmacological profile and distribution of various K2p channel subtypes in organisms are still being investigated. The Drosophila genome contains 11 subtypes; however, their function and expression profiles have not yet been determined. Doxapram is clinically used to enhance respiration in humans and blocks the acid-sensitive K2p TASK subtype in mammals. The resting membrane potential of larval Drosophila muscle and synaptic transmission at the neuromuscular junction are pH sensitive. The present study investigated the effects of doxapram on membrane potential and synaptic transmission using intracellular recordings of larval Drosophila muscles. Doxapram (1 mM and 10 mM) depolarizes the muscle and appears to depolarize motor neurons, causing an increase in the frequency of spontaneous quantal events and evoked excitatory junction potentials. Verapamil (1 and 10 mM) paralleled the action of doxapram. These changes were matched by an extracellular increase in KCl (50 mM) and blocked by Cd2+. It is assumed that the motor nerve depolarizes to open voltage-gated Ca2+ channels in presynaptic nerve terminals because of exposure to doxapram. These findings are significant for building models to better understand the function of pharmacological agents that affect K2p channels and how K2p channels contribute to the physiology of tissues. Drosophila offers a genetically amenable model that can alter the tissue-specific expression of K2p channel subtypes to simulate known human diseases related to this family of channels.


Assuntos
Doxapram , Drosophila , Animais , Humanos , Potenciais da Membrana , Drosophila/metabolismo , Doxapram/metabolismo , Doxapram/farmacologia , Junção Neuromuscular , Transmissão Sináptica , Canais de Potássio/metabolismo , Mamíferos/metabolismo
6.
J Cell Biol ; 222(1)2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36355348

RESUMO

Mechanisms that safeguard mitochondrial DNA (mtDNA) limit the accumulation of mutations linked to mitochondrial and age-related diseases. Yet, pathways that repair double-strand breaks (DSBs) in animal mitochondria are poorly understood. By performing a candidate screen for mtDNA repair proteins, we identify that REC-an MCM helicase that drives meiotic recombination in the nucleus-also localizes to mitochondria in Drosophila. We show that REC repairs mtDNA DSBs by homologous recombination in somatic and germline tissues. Moreover, REC prevents age-associated mtDNA mutations. We further show that MCM8, the human ortholog of REC, also localizes to mitochondria and limits the accumulation of mtDNA mutations. This study provides mechanistic insight into animal mtDNA recombination and demonstrates its importance in safeguarding mtDNA during ageing and evolution.


Assuntos
Reparo do DNA , DNA Mitocondrial , Proteínas de Drosophila , Animais , Humanos , Reparo do DNA/genética , DNA Mitocondrial/genética , Drosophila/genética , Proteínas de Drosophila/genética , Recombinação Homóloga , Meiose , Mitocôndrias/genética
7.
J Environ Sci (China) ; 125: 616-629, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36375944

RESUMO

The widely use of silver nanoparticles (AgNPs) as antimicrobial agents gives rise to potential environmental risks. AgNPs exposure have been reported to cause toxicity in animals. Nevertheless, the known mechanisms of AgNPs toxicity are still limited. In this study, we systematically investigated the toxicity of AgNPs exposure using Drosophila melanogaster. We show here that AgNPs significantly decreased Drosophila fecundity, the third-instar larvae weight and rates of pupation and eclosion in a dose-dependent manner. AgNPs reduced fat body cell viability in MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. AgNPs caused DNA damage in hemocytes and S2 cells. Interestingly, the mRNA levels of the entire metallothionein gene family were increased under AgNPs exposure as determined by RNA-seq analysis and validated by qRT-PCR, indicating that Drosophila responded to the metal toxicity of AgNPs by producing metallothioneins for detoxification. These findings provide a better understanding of the mechanisms of AgNPs toxicity and may provide clues to effect on other organisms, including humans.


Assuntos
Nanopartículas Metálicas , Prata , Humanos , Animais , Prata/toxicidade , Drosophila melanogaster/genética , Nanopartículas Metálicas/toxicidade , Espécies Reativas de Oxigênio , Drosophila , Mecanismos de Defesa
8.
Dev Comp Immunol ; 138: 104539, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36087786

RESUMO

Intestinal tissue functions in innate immunity to prevent the entry of harmful substances, and to maintain homeostasis through the constant proliferation of intestinal stem cells (ISC). To understand the mechanisms which regulate ISC in response to gut damage, we identified 81 differentially expressed genes (DEGs) through RNA-seq analysis after oral administration of three intestinal-damaging substances to Drosophila melanogaster. Through protein-protein interaction (PPI) and functional annotation studies, the top 22 DEGs ordered by the number of nodes in the PPI network were analyzed in relation to cell development. Through network topology analysis, we identified 12 essential seed genes. From this we confirmed that p53, RpL17, Fmr1, Stat92E, CG31343, Cnot4, CG9281, CG8184, Evi5, and to were essential for ISC proliferation during gut damage using knockdown RNAi Drosophila. This study presents a method for identifying candidate genes relating to intestinal damage that has scope for furthering our understanding of gut disease.


Assuntos
Proteínas de Drosophila , Drosophila , Animais , Proliferação de Células , Drosophila/genética , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Proteína do X Frágil de Retardo Mental/genética , Expressão Gênica , Genes Reguladores , Mapas de Interação de Proteínas , Células-Tronco , Proteína Supressora de Tumor p53/genética
9.
Life Sci Alliance ; 6(1)2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36379670

RESUMO

Membrane organelle function, localization, and proper partitioning upon cell division depend on interactions with the cytoskeleton. Whether membrane organelles also impact the function of cytoskeletal elements remains less clear. Here, we show that acute disruption of the ER around spindle poles affects mitotic spindle size and function in Drosophila syncytial embryos. Acute ER disruption was achieved through the inhibition of ER membrane fusion by the dominant-negative cytoplasmic domain of atlastin. We reveal that when centrosome-proximal ER membranes are disrupted, specifically at metaphase, mitotic spindles become smaller, despite no significant changes in microtubule dynamics. These smaller spindles are still able to mediate sister chromatid separation, yet with decreased velocity. Furthermore, by inducing mitotic exit, we found that nuclear separation and distribution are affected by ER disruption. Our results suggest that ER integrity around spindle poles is crucial for the maintenance of mitotic spindle shape and pulling forces. In addition, ER integrity also ensures nuclear spacing during syncytial divisions.


Assuntos
Proteínas de Drosophila , Fuso Acromático , Animais , Fuso Acromático/metabolismo , Centrossomo/metabolismo , Microtúbulos/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Retículo Endoplasmático/metabolismo , Drosophila/metabolismo
10.
Methods Mol Biol ; 2603: 187-198, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36370280

RESUMO

The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.


Assuntos
Drosophila melanogaster , Proteômica , Animais , Proteômica/métodos , Marcação por Isótopo/métodos , Drosophila melanogaster/genética , Drosophila , Processamento de Proteína Pós-Traducional
11.
Semin Cell Dev Biol ; 133: 107-122, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35396167

RESUMO

During morphogenesis, changes in the shapes of individual cells are harnessed to mold an entire tissue. These changes in cell shapes require the coupled remodeling of the plasma membrane and underlying actin cytoskeleton. In this review, we highlight cellularization of the Drosophila embryo as a model system to uncover principles of how membrane and actin dynamics are co-regulated in space and time to drive morphogenesis.


Assuntos
Actinas , Proteínas de Drosophila , Animais , Actinas/metabolismo , Drosophila/metabolismo , Embrião não Mamífero/metabolismo , Morfogênese , Proteínas de Drosophila/metabolismo , Membrana Celular/metabolismo , Drosophila melanogaster/metabolismo
12.
Mol Phylogenet Evol ; 178: 107653, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36404461

RESUMO

Cactophilic species of the Drosophila buzzatii cluster (repleta group) comprise an excellent model group to investigate genomic changes underlying adaptation to extreme climate conditions and host plants. In particular, these species form a tractable system to study the transition from chemically simpler breeding sites (like prickly pears of the genus Opuntia) to chemically more complex hosts (columnar cacti). Here, we report four highly contiguous genome assemblies of three species of the buzzatii cluster. Based on this genomic data and inferred phylogenetic relationships, we identified candidate taxonomically restricted genes (TRGs) likely involved in the evolution of cactophily and cactus host specialization. Functional enrichment analyses of TRGs within the buzzatii cluster identified genes involved in detoxification, water preservation, immune system response, anatomical structure development, and morphogenesis. In contrast, processes that regulate responses to stress, as well as the metabolism of nitrogen compounds, transport, and secretion were found in the set of species that are columnar cacti dwellers. These findings are in line with the hypothesis that those genomic changes brought about key mechanisms underlying the adaptation of the buzzatii cluster species to arid regions in South America.


Assuntos
Drosophila , Opuntia , Animais , Drosophila/genética , Filogenia , Melhoramento Vegetal , Adaptação Fisiológica/genética
13.
Semin Cell Dev Biol ; 136: 38-48, 2023 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-35595601

RESUMO

The ribosomal DNA (rDNA) in Drosophila is found as two additive clusters of individual 35 S cistrons. The multiplicity of rDNA is essential to assure proper translational demands, but the nature of the tandem arrays expose them to copy number variation within and between populations. Here, we discuss means by which a cell responds to insufficient rDNA copy number, including a historical view of rDNA magnification whose mechanism was inferred some 35 years ago. Recent work has revealed that multiple conditions may also result in rDNA loss, in response to which rDNA magnification may have evolved. We discuss potential models for the mechanism of magnification, and evaluate possible consequences of rDNA copy number variation.


Assuntos
Variações do Número de Cópias de DNA , Drosophila melanogaster , Animais , DNA Ribossômico/genética , Variações do Número de Cópias de DNA/genética , Drosophila melanogaster/genética , Drosophila/genética , Ribossomos
15.
J Genet ; 1012022.
Artigo em Inglês | MEDLINE | ID: mdl-36330785

RESUMO

Drosophila subobscura is characterized by a rich chromosomal polymorphism for inversions. Many inversions are adaptive to global warming and can be classified as 'warm' or 'cold' adapted. However, most studies were carried out from European populations located in the central area of the species distribution or from American colonizing populations. For this reason, we aimed to analyse the isolated and marginal Rasht population, located in the Hyrcanian forests area (Iran). The chromosomal polymorphism for inversions was compared with the previous Rasht samples (Rasht I and II) obtained 57 years ago. This polymorphism has changed based on the inversion composition and frequencies. Interestingly, the polymorphism for inversions was scarce and similar to that of Madeira, an isolated Atlantic island. Likely, this similarity is a consequence of the marginal location and isolation of the Rasht population. Also, the chromosomal thermal index (CTI) was 0.445, showing a significant increase over those from Rasht I (0.184) and II (0.210). All these observations were in agreement with the global warming expectations. Moreover, the CTI was also computed for Russian Caucasus and Turkish populations collected more than 40 years ago to better understand the adaptive potential of D. subobscura and to study the similarity between populations of different geographic areas. In summary, the inversions of D. subobscura also changed in marginal and isolated populations in agreement with the global warming expectations, and an open question is to know where is the threshold for this evolutionary change.


Assuntos
Inversão Cromossômica , Drosophila , Animais , Drosophila/genética , Irã (Geográfico) , Inversão Cromossômica/genética , Polimorfismo Genético , Cromossomos
16.
J Genet ; 1012022.
Artigo em Inglês | MEDLINE | ID: mdl-36330788

RESUMO

Males of Drosophila nepalensis show dimorphism in wing melanization, but how do they evolve and coordinate during evolution is unknown. Heterogeneity in the environment helps individuals to adapt accordingly either through genetic polymorphism or through phenotypic plasticity. In this study, we tried to untangle the genetic architecture underlying differences in wing melanization in males because in nature the frequency of spotted and spotless males is different. We investigated the genetic basis of the inheritance of the sexually dimorphic characteristic of wing spot area (WSA) in D. nepalensis males with the aid of genetic crosses. We found spot formation on wings in male is directly correlated to female body melanization. In addition to this, we studied the phenotypic plasticity in the degree of wing spot melanization in males with respect to changes in temperature. We observed that increased WSA is negatively correlated with higher temperature. We found dark and light females only at 21°C. Dark females always produced males with spotted wing, whereas lighter females always produced males without wing spots. Finally, we found wing spot is highly correlated in reciprocal progeny due to linkage or pleiotropy which could help in evolution.


Assuntos
Drosophila , Asas de Animais , Animais , Feminino , Masculino , Drosophila/genética , Caracteres Sexuais , Temperatura , Cruzamentos Genéticos
17.
J Genet ; 1012022.
Artigo em Inglês | MEDLINE | ID: mdl-36330787

RESUMO

The pioneering studies carried out on heat shock-induced synthesis of specific proteins in the early 1970s did not identify any Hsp60 family protein in Drosophila. By the early 1980s, although the members of Hsp60 family of heat shock proteins (Hsp) were identified in a wide range of eukaryotes as homologs of the bacterial GroEL, none was known in Drosophila. The existence of the Hsp60 family protein was serendipitously revealed in Drosophila in my laboratory in 1989. Contrary to the earlier reports that all tissues in flies display the canonical heat shock response, the larval Malpighian tubules (MT) did not show induction of any of the major Hsps but synthesis of a putative Hsp60 family protein was found to be the most abundant in this tissue. A few years later, we identified this MTspecific heat shock-induced protein to indeed be a member of the Hsp60/chaperonin family. The Drosophila genome sequence projects subsequently revealed four putative Hsp60 gene sequences in the D. melanogaster genome. The present historical perspective chronicles contributions from my and other laboratories that unraveled several aspects of intriguing biology of the multiple Hsp60 genes in D. melanogaster, and highlights challenging questions awaiting future studies.


Assuntos
Chaperonina 60 , Drosophila melanogaster , Animais , Chaperonina 60/genética , Chaperonina 60/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Drosophila/genética , Proteínas de Choque Térmico/genética , Resposta ao Choque Térmico , Proteínas de Choque Térmico HSP70/genética
18.
Sci Rep ; 12(1): 18346, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36319833

RESUMO

In Drosophila larvae, nociceptive mdIV sensory neurons detect diverse noxious stimuli and prompt a nociceptive rolling response. Intriguingly, the same neurons also regulate stereotyped larval movement. The channels responsible for transducing these stimuli into electric signals are not yet fully identified. Here we undertook genetic and electrophysiological analysis of Ppk19, a member of the Deg/ENaC family of cationic channels. ppk19 mutants exhibited an impaired nociceptive rolling response upon mechanical force and acid, but no impairment in response to noxious temperature and gentle touch. Mutants also exhibited defective larval movement. RNAi against ppk19 in mdIV neurons likewise produced larvae with defects in mechanical and acid nociception and larval movement, but no impairment in detection of heat and gentle touch. Cultured cells transfected with ppk19 produced currents in acid and hypotonic solution, suggesting that ppk19 encodes an ion channel that responds to acid and cell swelling. Taken together, these findings suggest that Ppk19 acts in mdIV neurons as a proton- and mechano-gated ion channel to mediate acid- and mechano-responsive nociception and larval movement.


Assuntos
Drosophila melanogaster , Drosophila , Animais , Drosophila melanogaster/genética , Prótons , Canais Iônicos , Larva/fisiologia , Células Receptoras Sensoriais
19.
Sci Rep ; 12(1): 19309, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36369211

RESUMO

Acetaminophen is the most common cause of acute drug-induced liver injury in the United States. However, research into the mechanisms of acetaminophen toxicity and the development of novel therapeutics is hampered by the lack of robust, reproducible, and cost-effective model systems. Herein, we characterize a novel Drosophila-based model of acetaminophen toxicity. We demonstrate that acetaminophen treatment of Drosophila results in similar pathophysiologic alterations as those observed in mammalian systems, including a robust production of reactive oxygen species, depletion of glutathione, and dose-dependent mortality. Moreover, these effects are concentrated in the Drosophila fat body, an organ analogous to the mammalian liver. Utilizing this system, we interrogated the influence of environmental factors on acetaminophen toxicity which has proven difficult in vertebrate models due to cost and inter-individual variability. We find that both increasing age and microbial depletion sensitize Drosophila to acetaminophen toxicity. These environmental influences both alter oxidative stress response pathways in metazoans. Indeed, genetic and pharmacologic manipulations of the antioxidant response modify acetaminophen toxicity in our model. Taken together, these data demonstrate the feasibility of Drosophila for the study of acetaminophen toxicity, bringing with it an ease of genetic and microbiome manipulation, high-throughput screening, and availability of transgenic animals.


Assuntos
Acetaminofen , Doença Hepática Induzida por Substâncias e Drogas , Animais , Acetaminofen/toxicidade , Acetaminofen/metabolismo , Drosophila/metabolismo , Estresse Oxidativo , Doença Hepática Induzida por Substâncias e Drogas/genética , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Glutationa/metabolismo , Fígado/metabolismo , Mamíferos/metabolismo
20.
Sci Rep ; 12(1): 19745, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36396856

RESUMO

Tracking and differentiating small insects at the individual levels requires appropriate marking materials because of their small size. This study proposes and investigates the use of fluorescent silica nanoparticles (FSNPs) as an internal marker owing to their good optical properties and biocompatibility. FSNPs were prepared using the water-in-oil reverse microemulsion technique with Rubpy dye as a fluorophore. The obtained particles were spherical, monodispersed in nanosize and exhibited bright orange luminescence under ultraviolet (UV) light. Internal marking was accomplished in fruit flies (Drosophila melanogaster) through feeding. The result shows that the fruit flies exhibit bright luminescence in their abdomen when exposed to UV light. The marking persistence duration of FSNPs in the fruit fly bodies is longer than those of other fluorescent dyes. Fruit flies fed with FSNPs have a longer lifespan than those fed with Rubpy dye. There was no difference in fertility and negative geotaxis response among the treatment and control groups. These findings demonstrate that FSNPs can be used as an internal marker in fruit flies, and are possibly applied with other small insects with a translucent abdomen.


Assuntos
Nanopartículas , Dióxido de Silício , Animais , Sobrevivência , Drosophila melanogaster/fisiologia , Corantes Fluorescentes , Drosophila , Fertilidade
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