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1.
BMC Health Serv Res ; 23(1): 106, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36726102

RESUMO

BACKGROUND: Organizational coaching to promote the implementation of evidence-informed interventions is becoming more popular in healthcare organizations. In order to open the "black box" of coaching for implementation, we first developed, then tested the rigor and utility of a model of coaching for implementation. METHODS: Interviews with nine experienced coaches were conducted and inductively coded to develop a model of coaching for implementation. Later, forty coaching calls with behavioral health organizations in Ohio, Wisconsin, and Florida were analyzed with directed content analysis using a priori codes based on this model. RESULTS: The coaching work that occurred during these calls aligned closely with the model of coaching for implementation developed by our team. Most coaching work was devoted to building capacity; almost as much work focused on building relationships. Very little coaching work was dedicated to building sustainability. Use of tools for organizational change and implementation remained relatively consistent across all coaching periods. CONCLUSION: Understanding what occurs during a successful coaching intervention will improve the effectiveness of coaching as an implementation strategy. Future research should focus on which processes and patterns make coaching more likely to promote specific implementation outcomes.


Assuntos
Tutoria , Humanos , Florida , Ohio
2.
PLoS One ; 18(1): e0276700, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36649279

RESUMO

COVID-19 is caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The severity of COVID-19 is highly variable and related to known (e.g., age, obesity, immune deficiency) and unknown risk factors. The widespread clinical symptoms encompass a large group of asymptomatic COVID-19 patients, raising a crucial question regarding genetic susceptibility, e.g., whether individual differences in immunity play a role in patient symptomatology and how much human leukocyte antigen (HLA) contributes to this. To reveal genetic determinants of susceptibility to COVID-19 severity in the population and further explore potential immune-related factors, we performed a genome-wide association study on 284 confirmed COVID-19 patients (cases) and 95 healthy individuals (controls). We compared cases and controls of European (EUR) ancestry and African American (AFR) ancestry separately. We identified two loci on chromosomes 5q32 and 11p12, which reach the significance threshold of suggestive association (p<1x10-5 threshold adjusted for multiple trait testing) and are associated with the COVID-19 susceptibility in the European ancestry (index rs17448496: odds ratio[OR] = 0.173; 95% confidence interval[CI], 0.08-0.36 for G allele; p = 5.15× 10-5 and index rs768632395: OR = 0.166; 95% CI, 0.07-0.35 for A allele; p = 4.25×10-6, respectively), which were associated with two genes, PPP2R2B at 5q32, and LRRC4C at 11p12, respectively. To explore the linkage between HLA and COVID-19 severity, we applied fine-mapping analysis to dissect the HLA association with mild and severe cases. Using In-silico binding predictions to map the binding of risk/protective HLA to the viral structural proteins, we found the differential presentation of viral peptides in both ancestries. Lastly, extrapolation of the identified HLA from the cohort to the worldwide population revealed notable correlations. The study uncovers possible differences in susceptibility to COVID-19 in different ancestral origins in the genetic background, which may provide new insights into the pathogenesis and clinical treatment of the disease.


Assuntos
COVID-19 , Predisposição Genética para Doença , Humanos , COVID-19/epidemiologia , COVID-19/genética , Florida , Estudo de Associação Genômica Ampla , Antígenos de Histocompatibilidade Classe I/genética , Antígenos HLA , SARS-CoV-2 , /genética
3.
PLoS One ; 18(1): e0280002, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36634115

RESUMO

The whitefly, Bemisia tabaci (Gennadius), is one of the most serious agricultural pests worldwide. Bemisia tabaci is a cryptic species complex of more than 40 species among which the invasive MEAM1 and MED species are the most widespread and economically important. Both MEAM1 and MED present intraspecific genetic variability and some haplotypes are reported to be more invasive than others. MED can be further deconstructed into different genetic groups, including MED-Q1 and MED-Q2. However, distinct biological phenotypes discerning the different MED mitochondrial haplotypes are yet to be characterized. Competitive displacement and life-history trials were carried out between MED-Q2 and MEAM1 populations collected in Florida, USA. In addition, a phylogenetic analysis was carried out including populations from previous whitefly competitive displacement studies for identification and comparison of the MED mitochondrial groups. In contrast to other studies with MED-Q1, the MED-Q2 population from Florida is less likely to displace MEAM1 on pepper. In addition, both pepper and watermelon were a more favorable host to MEAM1 compared to MED-Q2 according to the life history trials.


Assuntos
Hemípteros , Animais , Filogenia , Hemípteros/genética , Mitocôndrias/genética , Alimentos , Florida
5.
Sci Rep ; 13(1): 130, 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36599854

RESUMO

Laboratory and field-based studies of the invasive mosquito Aedes albopictus demonstrate its competency to transmit over twenty different pathogens linked to a broad range of vertebrate hosts. The vectorial capacity of Ae. albopictus to transmit these pathogens remains unclear, partly due to knowledge gaps regarding its feeding behavior. Blood meal analyses from field-captured specimens have shown vastly different feeding patterns, with a wide range of anthropophagy (human feeding) and host diversity. To address this knowledge gap, we asked whether differences in innate host preference may drive observed variation in Ae. albopictus feeding patterns in nature. Low generation colonies (F2-F4) were established with field-collected mosquitoes from three populations with high reported anthropophagy (Thailand, Cameroon, and Florida, USA) and three populations in the United States with low reported anthropophagy (New York, Maryland, and Virginia). The preference of these Ae. albopictus colonies for human versus non-human animal odor was assessed in a dual-port olfactometer along with control Ae. aegypti colonies already known to show divergent behavior in this assay. All Ae. albopictus colonies were less likely (p < 0.05) to choose the human-baited port than the anthropophilic Ae. aegypti control, instead behaving similarly to zoophilic Ae. aegypti. Our results suggest that variation in reported Ae. albopictus feeding patterns are not driven by differences in innate host preference, but may result from differences in host availability. This work is the first to compare Ae. albopictus and Ae. aegypti host preference directly and provides insight into differential vectorial capacity and human feeding risk.


Assuntos
Aedes , Animais , Odorantes , Comportamento Alimentar , Florida , Tailândia
6.
Sci Rep ; 13(1): 191, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36604450

RESUMO

The bald eagle (Haliaeetus leucocephalus) is a culturally and ecologically vital species in North America that embodies conservation success but continues to face threats that include emerging pathogens. The introduction of A/goose/Guangdong/1/1996 lineage highly pathogenic (HP) clade 2.3.4.4b H5N1 influenza A virus (IAV) in North America in late 2021 resulted in high rates of mortality among bald eagles. Here we show an alarming rate of bald eagle nest failure and mortality attributed to HP IAV. We documented fatal, systemic HP IAV infection in breeding adult and nestling bald eagles along the southeastern U.S. coast. Concurrently, annual bald eagle nest surveys in Georgia and Florida revealed a precipitous drop in success in coastal counties compared with previous years, portending negative impacts on population recruitment. As an apex predator and efficient scavenger, it is likely that bald eagles become infected through consumption of infected waterfowl. These results and similar reports of raptor mortality in Europe, Asia, and Africa, indicate a clear threat to raptor health. The possible long-term persistence of HP H5N1 IAV in North America poses an impending threat to bald eagle populations not only related to direct mortality but also decreased recruitment and warrants continued efforts to understand these potential impacts.


Assuntos
Águias , Virus da Influenza A Subtipo H5N1 , Animais , América do Norte/epidemiologia , Florida , Georgia
8.
PLoS One ; 18(1): e0278929, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36662888

RESUMO

America's unique response to the global COVID-19 pandemic has been both criticized and applauded across political and social spectrums. Compared to other developed nations, U.S. incidence and mortality rates were exceptionally high, due in part to inconsistent policies across local, state, and federal agencies regarding preventive behaviors like mask wearing and social distancing. Furthermore, vaccine hesitancy and conspiracy theories around COVID-19 and vaccine safety have proliferated widely, making herd immunity that much more challenging. What factors of the U.S. culture have contributed to the significant impact of the pandemic? Why have we not responded better to the challenges of COVID-19? Or would many people in the U.S. claim that we have responded perfectly well? To explore these questions, we conducted a qualitative and quantitative study of Florida State University faculty, staff, and students. This study measured their perceptions of the pandemic, their behaviors tied to safety and community, and how these practices were tied to beliefs of individualism and collectivism. We found that collectivist orientations were associated with a greater likelihood of wearing masks consistently, severe interruptions of one's social life caused by the pandemic, greater concern for infecting others, and higher levels of trust in medical professionals for behavioral guidelines surrounding the pandemic. These associations largely persist even after adjusting for political affiliation, which we find is also a strong predictor of COVID-19 beliefs and behaviors.


Assuntos
COVID-19 , Pandemias , Humanos , Pandemias/prevenção & controle , COVID-19/epidemiologia , COVID-19/prevenção & controle , Sudeste dos Estados Unidos/epidemiologia , Florida , Confiança
9.
Artigo em Inglês | MEDLINE | ID: mdl-36673968

RESUMO

Latinx trans and non-binary individuals (LTNB) face increased cancer-related health disparities. Studies evidence how barriers at the individual, provider and organizational levels drive cancer disparities among LTNB individuals. These barriers increase the emotional discomfort associated with testing and disengagement from cancer prevention efforts. Moreover, there are no guidelines or interventions that address cancer prevention specifically among LTNB individuals. There is a need to develop interventions informed by the LTNB communities to promote cancer prevention and screening. The study aims to describe the recommendations provided by LTNB individuals to foster cancer screening and prevention in the communities residing in Puerto Rico and Florida. We conducted two online focus groups with a total of 15 LTNB participants. Participants were recruited using non-probabilistic purposive sampling. We used rapid-qualitative analysis for data interpretation. Findings are gathered in three main themes: (1) recommendations for promoting cancer prevention screening among providers; (2) specific recommendations to promote cancer screening among LTBN individuals; and (3) recommendations on delivery formats to foster cancer prevention. These results evidence the need and feasibility of developing community informed tailored interventions targeting cancer screening and preventative care to reduce cancer-related health disparities among the LTNB population.


Assuntos
Neoplasias , Transexualidade , Humanos , Porto Rico/epidemiologia , Florida/epidemiologia , Neoplasias/prevenção & controle , Hispânico ou Latino/psicologia
10.
PLoS One ; 18(1): e0268221, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36719874

RESUMO

PURPOSE: Health disparities are driven by a complex interplay of determinants operating across multiple levels of influence. However, while recognized conceptually, much disparities research fails to capture this inherent complexity in study focus and/or design; little of such work accounts for the interplay across the multiple levels of influence from structural (contextual) to biological or clinical. We developed a novel modeling framework that addresses these challenges and provides new insights. METHODS: We used data from the Florida Cancer Data System on endometrial cancer patients and geocoded-derived social determinants of health to demonstrate the applicability of a new modeling paradigm we term PRISM regression. PRISM is a new highly interpretable tree-based modeling framework that allows for automatic discovery of potentially non-linear hierarchical interactions between health determinants at multiple levels and differences in survival outcomes between groups of interest, including through a new specific area-level disparity estimate (SPADE) incorporating these multilevel influences. RESULTS: PRISM demonstrates that hierarchical influences on racial disparity in endometrial cancer survival appear to be statistically relevant and that these better predict survival differences than only using individual level determinants. The interpretability of the models allows more careful inspection of the nature of these hierarchical effects on disparity. Additionally, SPADE estimates show distinct geographical patterns across census tracts in Florida. CONCLUSION: PRISM can provide a powerful new modeling framework with which to better understand racial disparities in cancer survival.


Assuntos
Neoplasias do Endométrio , Grupos Raciais , Feminino , Humanos , Fatores Raciais , Florida/epidemiologia , Endométrio
11.
Genet Test Mol Biomarkers ; 27(1): 1-4, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36719977

RESUMO

Purpose: Scalable solutions are needed to make pre-test genetic education about inherited cancer risk accessible across diverse and underserved populations. We evaluated an automated strategy to deliver genetic education through a web-based video among young Black females with breast cancer. Methods: 96 participants were recruited through state cancer registries in Florida and Tennesee. All participants viewed a 12 min video and completed a ten question quiz on inherited cancer knowledge before and after viewing the video. Median pre- and postvideo knowledge scores were categorized as <60% versus ≥60% and compared across demographic and clinical characteristics using binary logistic regression. Results: Of the 96 participants, mean age was 51, over 50% had income <$50 K, over 40% did not graduate college or have private insurance, and over 70% had previous genetic testing. Median knowledge scores significantly increased after viewing the video (p < 001), with no significant differences in those with or without prior testing. A higher post-video knowledge score was associated with an income ≥$50 K, a college degree, and private insurance (all p < .05). Conclusion: Among a population of young Black breast cancer patients, the educational video significantly increased knowledge. Findings support the use of automated pre-test educational tools as a scalable solution to make these services more accessible across populations.


Assuntos
Neoplasias da Mama , Humanos , Feminino , Pessoa de Meia-Idade , Neoplasias da Mama/genética , Neoplasias da Mama/epidemiologia , Testes Genéticos , Florida , Internet
12.
BMC Public Health ; 23(1): 79, 2023 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-36631768

RESUMO

BACKGROUND: Understanding geographic disparities in Coronavirus Disease 2019 (COVID-19) testing and outcomes at the local level during the early stages of the pandemic can guide policies, inform allocation of control and prevention resources, and provide valuable baseline data to evaluate the effectiveness of interventions for mitigating health, economic and social impacts. Therefore, the objective of this study was to identify geographic disparities in COVID-19 testing, incidence, hospitalizations, and deaths during the first five months of the pandemic in Florida.  METHODS: Florida county-level COVID-19 data for the time period March-July 2020 were used to compute various COVID-19 metrics including testing rates, positivity rates, incidence risks, percent of hospitalized cases, hospitalization risks, case-fatality rates, and mortality risks. High or low risk clusters were identified using either Kulldorff's circular spatial scan statistics or Tango's flexible spatial scan statistics and their locations were visually displayed using QGIS. RESULTS: Visual examination of spatial patterns showed high estimates of all COVID-19 metrics for Southern Florida. Similar to the spatial patterns, high-risk clusters for testing and positivity rates and all COVID-19 outcomes (i.e. hospitalizations and deaths) were concentrated in Southern Florida. The distributions of these metrics in the other parts of Florida were more heterogeneous. For instance, testing rates for parts of Northwest Florida were well below the state median (11,697 tests/100,000 persons) but they were above the state median for North Central Florida. The incidence risks for Northwest Florida were equal to or above the state median incidence risk (878 cases/100,000 persons), but the converse was true for parts of North Central Florida. Consequently, a cluster of high testing rates was identified in North Central Florida, while a cluster of low testing rate and 1-3 clusters of high incidence risks, percent of hospitalized cases, hospitalization risks, and case fatality rates were identified in Northwest Florida. Central Florida had low-rate clusters of testing and positivity rates but it had a high-risk cluster of percent of hospitalized cases. CONCLUSIONS: Substantial disparities in the spatial distribution of COVID-19 outcomes and testing and positivity rates exist in Florida, with Southern Florida counties generally having higher testing and positivity rates and more severe outcomes (i.e. hospitalizations and deaths) compared to Northern Florida. These findings provide valuable baseline data that is useful for assessing the effectiveness of preventive interventions, such as vaccinations, in various geographic locations in the state. Future studies will need to assess changes in spatial patterns over time at lower geographical scales and determinants of any identified patterns.


Assuntos
Teste para COVID-19 , COVID-19 , Humanos , Florida/epidemiologia , COVID-19/diagnóstico , COVID-19/epidemiologia , Nível de Saúde , Incidência
13.
PLoS Negl Trop Dis ; 17(1): e0011074, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36701264

RESUMO

Mathematical models have been widely used to study the population dynamics of mosquitoes as well as to test and validate the effectiveness of arbovirus outbreak responses and mosquito control strategies. The objective of this study is to assess the diel activity of mosquitoes in Miami-Dade, Florida, and Brownsville, Texas, the most affected areas during the Zika outbreak in 2016-2017, and to evaluate the effectiveness of simulated adulticide treatments on local mosquito populations. To assess variations in the diel activity patterns, mosquitoes were collected hourly for 96 hours once a month from May through November 2019 in Miami-Dade County, Florida, and Brownsville, Texas. We then performed a PERMANOVA followed by a SIMPER analysis to assess whether the abundance and species richness significantly varies at different hours of the day. Finally, we used a mathematical model to simulate the population dynamics of 5 mosquito vector species and evaluate the effectiveness of the simulated adulticide applications. A total of 14,502 mosquitoes comprising 17 species were collected in Brownsville and 10,948 mosquitoes comprising 19 species were collected in Miami-Dade County. Aedes aegypti was the most common mosquito species collected every hour in both cities and peaking in abundance in the morning and the evening. Our modeling results indicate that the effectiveness of adulticide applications varied greatly depending on the hour of the treatment. In both study locations, 9 PM was the best time for adulticide applications targeting all mosquito vector species; mornings/afternoons (9 AM- 5 PM) yielded low effectiveness, especially for Culex species, while at night (12 AM- 6 AM) the effectiveness was particularly low for Aedes species. Our results indicate that the timing of adulticide spraying interventions should be carefully considered by local authorities based on the ecology of the target mosquito species in the focus area.


Assuntos
Aedes , Arbovírus , Culex , Infecção por Zika virus , Zika virus , Animais , Infecção por Zika virus/epidemiologia , Florida/epidemiologia , Cidades , Mosquitos Vetores , Controle de Mosquitos/métodos
14.
Sci Rep ; 13(1): 258, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36604530

RESUMO

For reef framework to persist, calcium carbonate production by corals and other calcifiers needs to outpace loss due to physical, chemical, and biological erosion. This balance is both delicate and dynamic and is currently threatened by the effects of ocean warming and acidification. Although the protection and recovery of ecosystem functions are at the center of most restoration and conservation programs, decision makers are limited by the lack of predictive tools to forecast habitat persistence under different emission scenarios. To address this, we developed a modelling approach, based on carbonate budgets, that ties species-specific responses to site-specific global change using the latest generation of climate models projections (CMIP6). We applied this model to Cheeca Rocks, an outlier in the Florida Keys in terms of high coral cover, and explored the outcomes of restoration targets scheduled in the coming 20 years at this site by the Mission: Iconic Reefs restoration initiative. Additionally, we examined the potential effects of coral thermal adaptation by increasing the bleaching threshold by 0.25, 0.5, 1 and 2˚C. Regardless of coral adaptative capacity or restoration, net carbonate production at Cheeca Rocks declines heavily once the threshold for the onset of annual severe bleaching is reached. The switch from net accretion to net erosion, however, is significantly delayed by mitigation and adaptation. The maintenance of framework accretion until 2100 and beyond is possible under a decreased emission scenario coupled with thermal adaptation above 0.5˚C. Although restoration initiatives increase reef accretion estimates, Cheeca Rocks will only be able to keep pace with future sea-level rise in a world where anthropogenic CO2 emissions are reduced. Present results, however, attest to the potential of restoration interventions combined with increases in coral thermal tolerance to delay the onset of mass bleaching mortalities, possibly in time for a low-carbon economy to be implemented and complementary mitigation measures to become effective.


Assuntos
Antozoários , Animais , Antozoários/fisiologia , Recifes de Corais , Ecossistema , Florida , Carbonatos , Mudança Climática
15.
Ann Epidemiol ; 78: 19-27, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36563765

RESUMO

HIV transmission risk significantly increases at HIV viral load (VL) >1500 copies/mL. We sought to determine the percentage of person-time spent with VL >1500 copies/mL (pPT >1500) and the associations of demographic, clinical, and psychosocial factors and this outcome among persons with HIV receiving care. A retrospective analysis of data from clients enrolled in the Ryan White Program from 2017 to 2019 was performed. We assessed pPT >1500 in HIV care by utilizing consecutive VL pairs and calculating the length of time between each pair and the corresponding time spent for the observation period. The association between pPT >1500 and selected client characteristics were analyzed using a random-effects zero-inflated negative binomial model. Among the 6390 clients, 42% were aged 50 or older, 52% MSM, and 59% Hispanic. Overall, 7.5% of clients spent, on average, 27.4 days per year at substantial risk of transmitting HIV. Younger age, AIDS diagnosis, and reported drug use in the preceding 12 months were associated with higher pPT >1500. Tailored interventions should be implemented to meet the unique HIV needs of groups with consistent viremia to significantly minimize transmission risk.


Assuntos
Infecções por HIV , Transtornos Relacionados ao Uso de Substâncias , Humanos , Florida/epidemiologia , Infecções por HIV/diagnóstico , Estudos Retrospectivos , Carga Viral
16.
Accid Anal Prev ; 181: 106933, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36577242

RESUMO

Wrong-Way Driving (WWD) crashes are relatively rare but more likely to produce fatalities and severe injuries than other crashes. WWD crash segment prediction task is challenging due to its rare nature, and very few roadway segments experience WWD events. WWD crashes involve complex interactions among roadway geometry, vehicle, environment, and drivers, and the effect of these complex interactions is not always observable and measurable. This study applied two advanced Machine Learning (ML) models to overcome the imbalanced dataset problem and identified local and global factors contributing to WWD crash segments. Five years (2015-2019) of WWD crash data from Florida state were used in this study for WWD model development. The first modeling approach applied four different hybrid data augmentation techniques to the training dataset before applying the XGBoost classification algorithm. In the second model, a rare event modeling approach using the Autoencoder-based anomaly detection method was applied to the original data to identify WWD roadway segments. A third model was applied based on the statistical method to compare the performance of ML models in predicting the WWD segments. The performance comparison of the adopted models showed that the XGBoost model with the Adaptive Synthetic Sampling (ADASYN) method performed best in terms of precision and recall values compared to the autoencoder-based anomaly detection method. The best-performing model was used for the feature analysis with an interpretable machine-learning technique. The SHapley Additive exPlanations (SHAP) values showed that high-intensity developed land use, length of roadway, log of Annual Average Daily traffic (AADT), and lane width were positively associated with WWD roadway segments. The results of this study can be used to deploy WWD countermeasures effectively.


Assuntos
Acidentes de Trânsito , Condução de Veículo , Humanos , Florida
17.
J Environ Manage ; 326(Pt B): 116806, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36410149

RESUMO

Most studies have explored the Covid-19 outbreak by mainly focusing on restrictive public policies, human health, and behaviors at the macro level. However, the impacts of built and socio-economic environments, accounting for spatial effects on the spread at the local levels, have not been thoroughly studied. In this study, the relationships between the spatial spread of the virus and various indicators of the built and socio-economic environments are investigated, using Florida ZIP-code data on accumulated cases before large-scale vaccination campaigns began in 2021. Spatial regression models are used to account for the spatial dependencies and interactions that are core factors in Covid-19 spread. This study reveals both the spillover dynamics of the coronavirus spread at the ZIP code level and the existence of spatial dependencies among the unobserved variables represented by the error term. In addition, the findings show a positive association between the expected number of Covid-19 cases and specific land uses, such as education facilities and retail densities. Finally, the study highlights critical socio-economic characteristics causing a substantial increase in Covid-19 spread. Such results could help policymakers, public health experts, and urban planners design strategies to mitigate the spread of future Covid-19-like diseases.


Assuntos
COVID-19 , Meio Ambiente , Fatores Socioeconômicos , Humanos , COVID-19/epidemiologia , COVID-19/transmissão , Florida/epidemiologia , Análise Espacial , Densidade Demográfica
18.
Antimicrob Agents Chemother ; 67(1): e0128422, 2023 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-36541772

RESUMO

In the United States, vanB-mediated resistance in enterococci is rare. We characterized three sequence type (ST) 6, vancomycin-resistant Enterococcus faecalis isolates causing bacteremia in unique patients in spatiotemporally distinct settings. Isolates were recovered between 2018 and 2020 in two cities in the United States (Houston, TX; Miami, FL). The isolates harbored the vanB operon on a chromosomally located Tn1549 transposon, and epidemiological data suggested multiple introductions of the vanB gene cluster into ST6 E. faecalis.


Assuntos
Enterococcus faecium , Infecções por Bactérias Gram-Positivas , Enterococos Resistentes à Vancomicina , Humanos , Enterococcus faecalis/genética , Resistência a Vancomicina/genética , Florida/epidemiologia , Texas/epidemiologia , Enterococos Resistentes à Vancomicina/genética , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Infecções por Bactérias Gram-Positivas/epidemiologia , Proteínas de Bactérias/genética , Antibacterianos/farmacologia
19.
J Nat Prod ; 86(1): 182-190, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36580354

RESUMO

Previous chemical investigation of the Irish deep-sea soft coral Duva florida led to the identification of tuaimenal A (10), a new merosesquiterpene containing a highly substituted chromene core and modest cytotoxicity against cervical cancer. Further MS/MS and NMR-guided investigation of this octocoral has resulted in the isolation and characterization of seven additional tuaimenal analogs, B-H (1-7), as well as two known A-ring aromatized steroids (8, 9), and additional tuaimenal A (10). Tuaimenals B, F, and G (1, 5, 6), bearing an oxygen at the C5 position, as well as monocyclic tuaimenal H (7), show increased cervical cancer inhibition profiles in comparison to that of 10. Tuaimenal G further displayed potent, selective cytotoxicity with an EC50 value of 0.04 µM against the C33A cell line compared to the CaSki cell line (EC50 20 µM). These data reveal the anticancer properties of tuaimenal analogs and suggest unique antiproliferation mechanisms across these secondary metabolites.


Assuntos
Antozoários , Neoplasias do Colo do Útero , Animais , Humanos , Feminino , Antozoários/química , Neoplasias do Colo do Útero/tratamento farmacológico , Espectrometria de Massas em Tandem , Florida , Linhagem Celular Tumoral
20.
Integr Environ Assess Manag ; 18(1): 148-162, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34546640

RESUMO

Over the past decade, the scientific community has studied, experimented, and published a notable body of literature on the ecological enhancement of coastal and marine infrastructure (CMI). The Nature-Inclusive Design (NID) approach refers to methods and technologies that can be integrated into the design and construction of CMI to create a suitable habitat for native species (or communities) whose natural habitat has been degraded or reduced. To examine the compliance of new environmentally sensitive technologies with structural requirements and fiscal restraints, while providing ecosystem and habitat value, this paper presents the findings of a structural-economical-biological analysis of ecologically engineered Articulated Concrete Block Mattresses (ACBMs). To evaluate the structural and biological performance of the Ecological Articulated Concrete Block Mattresses, a pilot project was deployed in April 2017 at Port Everglades, Florida, USA, and evaluated against controls of adjacent artificial structures and smooth-surface concrete blocks and monitored over a period of two years. The elements of ecological enhancement implemented in the fabrication and design of the ecologically enhanced ACBMs were comprised of bio-enhancing concrete additives and science-based designs. Based on the results of this study, these design alterations have increased the richness and diversity of sessile assemblages compared to control blocks and adjacent artificial structures and supported a higher abundance of mobile species. This ecological improvement was achieved within the operational limitations of conventional manufacturing and installation technologies, while complying with strict structural requirements for standard concrete marine construction. The results supported the working hypothesis and demonstrated that modifications of concrete composition, surface texture, and macro-design have the potential to increase the ecological value of concrete-based CMI and promote a more sustainable and adaptive approach to coastal and marine development in an era of climate resilience-building. Integr Environ Assess Manag 2022;18:148-162. © 2021 SETAC.


Assuntos
Leitos , Ecossistema , Florida , Projetos Piloto
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