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1.
Carbohydr Polym ; 339: 122202, 2024 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-38823898

RESUMO

Interactions among multi-component play a critical role in modulating the foaming properties of aerated foods. This study evaluated the mechanisms of synergistic improvement of gellan gum (GEG) and guar gum (GUG) on the foaming properties of soy protein isolate (SPI)-based complex. The results showed that the GEG/GUG ratio was closely related to the intermolecular interactions of SPI-based ternary complex and the dynamical changing of its foaming properties. The SPI/GEG/GUG ternary complex with a GEG/GUG ratio of 2/3 exhibited the highest foamability (195 %) and comparable foam stability (99.17 %), which were 32.95 % and 2.99 % higher than that of SPI/GEG binary complex. At this ratio, GUG promoted the interactions between SPI and GEG, and bound to complex's surface through hydrogen bonding, resulting in the increase of particle size and surface charge, and the decrease of surface hydrophobicity. Although this reduced the diffusion of complex onto the air/water interface, it increased permeation rate and molecular rearrangement behavior, which were the potential mechanisms to improve the foaming properties. Additionally, the synergistic effect of GEG and GUG also enhanced the elastic strength and solid characteristics of foam systems. This study provided a theoretical guidance for the targeted modulation of foaming properties of multi-component aerated foods.


Assuntos
Galactanos , Mananas , Gomas Vegetais , Polissacarídeos Bacterianos , Proteínas de Soja , Gomas Vegetais/química , Galactanos/química , Proteínas de Soja/química , Mananas/química , Polissacarídeos Bacterianos/química , Interações Hidrofóbicas e Hidrofílicas , Tamanho da Partícula , Ligação de Hidrogênio
2.
Carbohydr Polym ; 339: 122268, 2024 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-38823931

RESUMO

The influence of locust bean gum (LBG) galactomannans (GMs) molecular weight (Mw) to assemble microparticulate systems was evaluated, and carriers for deep lung delivery were developed. A commercial batch of LBG with a mannose/galactose (M/G) ratio of 2.4 (batch 1) was used to study the influence of different microwave partial acid hydrolysis conditions on carbohydrate composition, glycosidic linkages, and aqueous solutions viscosity. The microwave treatment did not affect the composition, presenting 4-Man (36-42 %), 4,6-Man (27-35 %), and T-Gal (24-25 %) as the main glycosidic linkages. Depolymerization led to a viscosity reduction (≤0.005 Pa·s) with no major impact on polysaccharide debranching. The structural composition of the LBG galactomannans were further elucidated with sequence-specific proteins using carbohydrate microarray technologies. A second batch of LBG (M/G 3.3) was used to study the impact of GMs with different Mw on microparticle assembling, characteristics, and insulin release kinetics. The low-Mw GMs microparticles led to a faster release (20 min) than the higher-Mw (40 min) ones, impacting the release kinetics. All microparticles exhibited a safety profile to cells of the respiratory tract. However, only the higher-Mw GMs allowed the assembly of microparticles with sizes suitable for this type of administration.


Assuntos
Galactose , Mananas , Peso Molecular , Gomas Vegetais , Mananas/química , Galactose/química , Galactose/análogos & derivados , Gomas Vegetais/química , Humanos , Pulmão/metabolismo , Portadores de Fármacos/química , Tamanho da Partícula , Viscosidade , Insulina/química , Insulina/administração & dosagem , Liberação Controlada de Fármacos , Galactanos/química , Manose/química , Animais
3.
J Environ Sci (China) ; 144: 1-14, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38802222

RESUMO

Nanoscale zero-valent iron (NZVI), which has the advantages of small particle size, large specific surface area, and high reactivity, is often injected into contaminated aquifers in the form of slurry. However, the prone to passivation and agglomeration as well as poor stability and mobility of NZVI limit the further application of this technology in fields. Therefore, sulfided NZVI loaded on reduced graphene oxide (S-NZVI/rGO) and guar gum (GG) with shear-thinning properties as stabilizers were used to synthesize S-NZVI/rGO@GG slurries. SEM, TEM, and FT-IR confirmed that the dispersion and anti-passivation of NZVI were optimized in the coupled system. The stability and mobility of the slurry were improved by increasing the GG concentration, enhancing the pH, and decreasing the ionic strength and the presence of Ca2+ ions, respectively. A modified advection-dispersion equation (ADE) was used to simulate the transport experiments considering the strain and physicochemical deposition/release. Meanwhile, colloidal filtration theory (CFT) demonstrated that Brownian motion plays a dominant role in the migration of S-NZVI/rGO@GG slurry, and the maximum migration distance can be increased by appropriately increasing the injection rate. Extended-Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory showed that the excellent stability and migration of S-NZVI/rGO@GG slurry mainly came from the GG spatial forces. This study has important implications for the field injection of S-NZVI/rGO@GG slurry. According to the injection parameters, the injection range of S-NZVI/rGO@GG slurry is effectively controlled, which lays the foundation for the promotion of application in actual fields.


Assuntos
Galactanos , Grafite , Ferro , Mananas , Gomas Vegetais , Grafite/química , Gomas Vegetais/química , Galactanos/química , Mananas/química , Ferro/química , Modelos Químicos , Nanopartículas Metálicas/química
4.
Int J Biol Macromol ; 269(Pt 1): 132051, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38777687

RESUMO

The impact of guar gum (GG), crude algae ethanolic extract (CAEE), and turmeric essential oil (TEO) incorporated edible coating formulations on the quality of cut potatoes was investigated at room temperature (27 ±â€¯3 °C, 70-85 % RH) storage using a rotatable central composite design. Besides, 30 % glycerol, 5 % calcium chloride, and 3 % ascorbic acid (w/w) were added to the coating solution as additives. The surface color, respiration rate, water vapor transmission rate, visible mold growth, and sensory analysis were assessed after seven days of storage. The inclusion of ascorbic acid and TEO in edible coating demonstrated a more effective delay in browning. The coated potatoes had lower OTR, CTR, and WVTR values for GG concentrations of 0.5 to 1 g/100 mL than the control. Compared to additives, higher concentrations of GG improved response parameters. The WVTR value of coated potatoes was significantly impacted by the interaction between CAEE and TEO with GG. Incorporating CAEE and TEO into the formulations of guar gum led to a reduction in the permeability of the coating to oxygen and water vapor. The seven days of extended shelf life compared to two days of control were observed with the optimized coating formulation. Furthermore, the application of the coating treatment proved effective in preventing enzymatic browning and creating a barrier against moisture and gases, contributing to prolonged freshness during extended storage periods.


Assuntos
Armazenamento de Alimentos , Galactanos , Mananas , Gomas Vegetais , Solanum tuberosum , Gomas Vegetais/química , Galactanos/química , Mananas/química , Mananas/farmacologia , Solanum tuberosum/química , Armazenamento de Alimentos/métodos , Conservação de Alimentos/métodos
5.
Int J Immunopathol Pharmacol ; 38: 3946320241249429, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38721971

RESUMO

OBJECTIVE: This study investigated the raft-forming suspension of famotidine as an anti-reflux formulation to improve the oral bioavailability of narrow absorption window drugs by enhancing gastric residence time (GRT) and preventing gastro-esophageal reflux disease (GERD). METHOD: Various combinations of raft-forming agents, such as Tragacanth gum (TG), guar gum (GG), and xanthan gum (XG), were evaluated alongside sodium alginate (SA) to develop an effective raft. Preformulation studies and preliminary screening were conducted to identify the most suitable raft-forming agent, and GG was chosen due to its mucilaginous properties. The formulation was optimized using a 32 full factorial design, with the quantities of GG and SA as independent factors and apparent viscosity and in-vitro drug release (%) as dependent factors. The in vivo floating behavior study was performed for optimized and stabilized formulation. RESULTS: Among the tested batches, F6 was selected as the optimized formulation. It exhibited desirable characteristics such as adequate raft weight for extended floating in gastric fluid, improved apparent viscosity, and a significant percentage of drug release at 12 h. A mathematical model was applied to the in-vitro data to gain insights into the drug release mechanism of the formulation. The stability of the suspension was assessed under accelerated conditions, and it demonstrated satisfactory stability. The formulation remains floating in the Rabbit stomach for more than 12 h. CONCLUSION: It concludes that the developed formulation has enhanced bioavailability in the combination of GG and SA. The floating layer of the raft prevents acid reflux, and the famotidine is retained for an extended period of time in the gastric region, preventing excess acid secretion. The developed formulations are effective for stomach ulcers and GERD, with the effect of reducing acid secretion by H2 receptor antagonists.


Assuntos
Sistemas de Liberação de Medicamentos , Famotidina , Galactanos , Famotidina/administração & dosagem , Famotidina/farmacocinética , Animais , Sistemas de Liberação de Medicamentos/métodos , Liberação Controlada de Fármacos , Alginatos , Refluxo Gastroesofágico/tratamento farmacológico , Refluxo Gastroesofágico/metabolismo , Disponibilidade Biológica , Mananas/administração & dosagem , Gomas Vegetais , Viscosidade , Masculino , Coelhos , Mucosa Gástrica/metabolismo , Mucosa Gástrica/efeitos dos fármacos , Polissacarídeos Bacterianos , Estabilidade de Medicamentos , Administração Oral
6.
Carbohydr Polym ; 338: 122201, 2024 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-38763726

RESUMO

Agarans represent a group of galactans extracted from red algae. Funoran and agarose are the two major types and commercially applied polysaccharides of agaran. Although the glycoside hydrolases targeting ß-glycosidic bonds of agaran have been widely investigated, those capable of degrading α-glycosidic bonds of agarose were limited, and the enzyme degrading α-linkages of funoran has not been reported till now. In this study, a GH96 family enzyme BiAF96A_Aq from a marine bacterium Aquimarina sp. AD1 was heterologously expressed in Escherichia coli. BiAF96A_Aq exhibited dual activities towards the characteristic structure of funoran and agarose, underscoring the multifunctionality of GH96 family members. Glycomics and NMR analysis revealed that BiAF96A_Aq hydrolyzed the α-1,3 glycosidic bonds between 3,6-anhydro-α-l-galactopyranose (LA) and ß-d-galactopyranose-6-sulfate (G6S) of funoran, as well as LA and ß-d-galactopyranose (G) of agarose, through an endo-acting manner. The end products of BiAF96A_Aq were majorly composed of disaccharides and tetrasaccharides. The identification of the activity of BiAF96A_Aq on funoran indicated the first discovery of the funoran hydrolase for α-1,3 linkage. Considering the novel catalytic reaction, we proposed to name this activity as "α-funoranase" and recommended the assignment of a dedicated EC number for its classification.


Assuntos
Glicosídeo Hidrolases , Sefarose , Sefarose/química , Glicosídeo Hidrolases/química , Glicosídeo Hidrolases/metabolismo , Glicosídeo Hidrolases/genética , Hidrólise , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Escherichia coli/genética , Galactanos/química , Galactanos/metabolismo
7.
Int J Biol Macromol ; 270(Pt 1): 132035, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38705316

RESUMO

The frequently encountered wastewater contaminations, including soluble aromatic compound and dye pollutants, pathogenic bacteria, and insoluble oils, have resulted in significant environmental and human health issues. It poses a challenge to utilize identical materials for the treatment of complex wastewater. Herein, in this research, multifunctional Ag NPs/guar gum hybrid hydrogels were fabricated using a facile in situ reduction and self-crosslinking method for efficient remediation of complex wastewater. The Ag NPs/guar gum hybrid hydrogel showed remarkable remodeling, adhesive, and self-healing characteristics, which was favorable for its versatile applications. The combination of Ag NPs with the guar gum skeleton endowed the hybrid hydrogel with exceptional catalytic activity for reducing aromatic compounds and dye pollutants, as well as remarkable antibacterial efficacy against pathogenic bacteria. In addition, the Ag NPs/guar gum hybrid hydrogel could be employed to coat a variety of substrates, including cotton fabrics and stainless steel meshes. The hydrogel coated cotton fabrics and meshes presented superhydrophilicity/underwater superoleophobicity, excellent antifouling capacity, and outstanding recyclability, which could be successfully applied for efficient separation of oil-water mixtures. The findings of this work provide a feasible and cost-effective approach for the remediation of intricate wastewater.


Assuntos
Antibacterianos , Galactanos , Hidrogéis , Mananas , Nanopartículas Metálicas , Gomas Vegetais , Prata , Galactanos/química , Gomas Vegetais/química , Prata/química , Mananas/química , Antibacterianos/química , Antibacterianos/farmacologia , Catálise , Nanopartículas Metálicas/química , Hidrogéis/química , Águas Residuárias/química , Purificação da Água/métodos , Água/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/isolamento & purificação , Óleos/química
8.
Carbohydr Polym ; 337: 122188, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38710565

RESUMO

Growing plants in karst areas tends to be difficult due to the easy loss of water and soil. To enhance soil agglomeration, water retention, and soil fertility, this study developed a physically and chemically crosslinked hydrogel prepared from quaternary ammonium guar gum and humic acid. The results showed that non-covalent dynamic bonds between the two components delayed humic acid release into the soil, with a release rate of only 35 % after 240 h. The presence of four hydrophilic groups (quaternary ammonium, hydroxyl, carboxyl, and carbonyl) in the hydrogel more than doubled the soil's water retention capacity. The interaction between hydrogel and soil minerals (especially carbonate and silica) promoted hydrogel-soil and soil­carbonate adhesion, and the adhesion strength between soil particles was enhanced by 650 %. Moreover, compared with direct fertilization, this degradable hydrogel not only increased the germination rate (100 %) and growth status of mung beans but also reduced the negative effects of excessive fertilization on plant roots. The study provides an eco-friendly, low-cost, and intelligent system for soil improvement in karst areas. It further proves the considerable application potential of hydrogels in agriculture.


Assuntos
Galactanos , Substâncias Húmicas , Hidrogéis , Mananas , Gomas Vegetais , Compostos de Amônio Quaternário , Solo , Gomas Vegetais/química , Galactanos/química , Mananas/química , Hidrogéis/química , Solo/química , Compostos de Amônio Quaternário/química , Fertilizantes , Preparações de Ação Retardada/química , Germinação/efeitos dos fármacos , Água/química
9.
J Med Invest ; 71(1.2): 121-128, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38735707

RESUMO

OBJECTIVES: Partially hydrolyzed guar gum (PHGG) is a soluble dietary fiber;in addition to improving bowel movements, it maintains intestinal health by producing short-chain fatty acids. However, majority of clinical studies on PHGG have been concluded within a month and excluded usual drug therapy. Hence, this study aimed to determine the effects of long-term consumption of PHGG, in combination with drug therapy, on gut bacteria ratios, laboratory values for inflammatory response, and fecal characteristics. METHODS AND RESULTS: The study was performed in patients with irritable bowel syndrome (IBS), Crohn's disease (CD), and ulcerative colitis (UC), by the administration of PHGG for six months while they continued their usual treatment. PHGG treatment caused significant changes in patients with IBS, including an increase in the abundance of short-chain fatty acid-producing bacteria, a significant decrease in Bacteroides abundance, and normalization of the Bristol scale of stool. In patients with UC, non-significant normalization of soft stools and decrease in fecal calprotectin were observed. Adverse events were not observed in any of the groups. CONCLUSION: Thus, it would be beneficial to include PHGG in the usual drug therapies of patients with IBS. J. Med. Invest. 71 : 121-128, February, 2024.


Assuntos
Fibras na Dieta , Galactanos , Microbioma Gastrointestinal , Síndrome do Intestino Irritável , Mananas , Gomas Vegetais , Humanos , Microbioma Gastrointestinal/efeitos dos fármacos , Síndrome do Intestino Irritável/tratamento farmacológico , Síndrome do Intestino Irritável/microbiologia , Masculino , Feminino , Fibras na Dieta/administração & dosagem , Adulto , Pessoa de Meia-Idade , Mananas/administração & dosagem , Gomas Vegetais/administração & dosagem , Galactanos/administração & dosagem , Doenças Inflamatórias Intestinais/tratamento farmacológico , Fezes/microbiologia , Fezes/química , Ácidos Graxos Voláteis/análise , Ácidos Graxos Voláteis/metabolismo
10.
Langmuir ; 40(19): 10305-10312, 2024 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-38696716

RESUMO

The limited elasticity and inadequate bonding of hydrogels made from guar gum (GG) significantly hinder their widespread implementation in personalized wearable flexible electronics. In this study, we devise GG-based self-adhesive hydrogels by creating an interpenetrating network of GG cross-linked with acrylic, 4-vinylphenylboronic acid, and Ca2+. With the leverage of the dynamic interactions (hydrogen bonds, borate ester bonds, and coordination bonds) between -OH in GG and monomers, the hydrogel exhibits a high stretchability of 700%, superior mechanical stress of 110 kPa, and robust adherence to several substrates. The adhesion strength of 54 kPa on porcine skin is obtained. Furthermore, the self-adhesive hydrogel possesses stable conductivity, an elevated gauge factor (GF), and commendable durability. It can be affixed to the human body as a strain sensor to obtain precise monitoring of human movement behavior. Our research offers possibilities for the development of GG-based hydrogels and applications in wearable electronics and medical monitoring.


Assuntos
Condutividade Elétrica , Galactanos , Hidrogéis , Mananas , Gomas Vegetais , Hidrogéis/química , Mananas/química , Gomas Vegetais/química , Galactanos/química , Animais , Dispositivos Eletrônicos Vestíveis , Humanos , Suínos , Adesivos/química
11.
Biomacromolecules ; 25(6): 3432-3448, 2024 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-38771294

RESUMO

Preventing bacterial infections is a crucial aspect of wound healing. There is an urgent need for multifunctional biomaterials without antibiotics to promote wound healing. In this study, we fabricated a guar gum (GG)-based nanocomposite hydrogel, termed GBTF, which exhibited photothermal antibacterial therapy for infected wound healing. The GBTF hydrogel formed a cross-linked network through dynamic borate/diol interactions between GG and borax, thereby exhibiting simultaneously self-healing, adaptable, and injectable properties. Additionally, tannic acid (TA)/Fe3+ nanocomplexes (NCs) were incorporated into the hydrogel to confer photothermal antibacterial properties. Under the irradiation of an 808 nm near-infrared laser, the TA/Fe3+ NCs in the hydrogel could rapidly generate heat, leading to the disruption of bacterial cell membranes and subsequent bacterial eradication. Furthermore, the hydrogels exhibited good cytocompatibility and hemocompatibility, making them a precandidate for preclinical and clinical applications. Finally, they could significantly promote bacteria-infected wound healing by reducing bacterial viability, accelerating collagen deposition, and promoting epithelial remodeling. Therefore, the multifunctional GBTF hydrogel, which was composed entirely of natural substances including guar gum, borax, and polyphenol/ferric ion NCs, showed great potential for regenerating infected skin wounds in clinical applications.


Assuntos
Antibacterianos , Galactanos , Hidrogéis , Mananas , Nanocompostos , Terapia Fototérmica , Gomas Vegetais , Cicatrização , Mananas/química , Mananas/farmacologia , Gomas Vegetais/química , Gomas Vegetais/farmacologia , Galactanos/química , Galactanos/farmacologia , Cicatrização/efeitos dos fármacos , Antibacterianos/farmacologia , Antibacterianos/química , Nanocompostos/química , Hidrogéis/química , Hidrogéis/farmacologia , Animais , Terapia Fototérmica/métodos , Camundongos , Taninos/química , Taninos/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Humanos , Escherichia coli/efeitos dos fármacos , Boratos
12.
Gut Microbes ; 16(1): 2341457, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38630030

RESUMO

With an increasing interest in dietary fibers (DFs) to promote intestinal health and the growth of beneficial gut bacteria, there is a continued rise in the incorporation of refined DFs in processed foods. It is still unclear how refined fibers, such as guar gum, affect the gut microbiota activity and pathogenesis of inflammatory bowel disease (IBD). Our study elucidated the effect and underlying mechanisms of guar gum, a fermentable DF (FDF) commonly present in a wide range of processed foods, on colitis development. We report that guar gum containing diet (GuD) increased the susceptibility to colonic inflammation. Specifically, GuD-fed group exhibited severe colitis upon dextran sulfate sodium (DSS) administration, as evidenced by reduced body weight, diarrhea, rectal bleeding, and shortening of colon length compared to cellulose-fed control mice. Elevated levels of pro-inflammatory markers in both serum [serum amyloid A (SAA), lipocalin 2 (Lcn2)] and colon (Lcn2) and extensive disruption of colonic architecture further affirmed that GuD-fed group exhibited more severe colitis than control group upon DSS intervention. Amelioration of colitis in GuD-fed group pre-treated with antibiotics suggest a vital role of intestinal microbiota in GuD-mediated exacerbation of intestinal inflammation. Gut microbiota composition and metabolite analysis in fecal and cecal contents, respectively, revealed that guar gum primarily enriches Actinobacteriota, specifically Bifidobacterium. Guar gum also altered multiple genera belonging to phyla Bacteroidota and Firmicutes. Such shift in gut microbiota composition favored luminal accumulation of intermediary metabolites succinate and lactate in the GuD-fed mice. Colonic IL-18 and tight junction markers were also decreased in the GuD-fed group. Importantly, GuD-fed mice pre-treated with recombinant IL-18 displayed attenuated colitis. Collectively, unfavorable changes in gut microbiota activity leading to luminal accumulation of lactate and succinate, reduced colonic IL-18, and compromised gut barrier function following guar gum feeding contributed to increased colitis susceptibility.


Guar gum increased susceptibility to colitisGuar gum-induced exacerbation of colitis is gut microbiota dependentGuar gum-induced shift in microbiota composition favored the accumulation of luminal intermediate metabolites succinate and lactateGuar gum-fed mice exhibited reduced colonic level of IL-18 and tight junction molecules.Exogenous IL-18 administration partly rescued mice from guar gum-induced colitis susceptibility.


Assuntos
Colite , Galactanos , Microbioma Gastrointestinal , Mananas , Gomas Vegetais , Animais , Camundongos , Interleucina-18 , Inflamação , Colite/induzido quimicamente , Fibras na Dieta , Ácido Láctico , Succinatos
13.
Int J Biol Macromol ; 268(Pt 1): 131583, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38621554

RESUMO

This study evaluated the foaming properties, the dynamic adsorption behavior at the air/water (A/W) interface and the foam rheological characteristics of complexes formed by soy protein isolate (SPI) and different charged polysaccharides, including chitosan (CS), guar gum (GUG) and gellan gum (GEG). The results showed that the SPI/CS10 had the highest initial foam volume (26.67 mL), which were 3.89 %, 100.08 % and 70.19 % higher than that of single SPI, SPI/GUG and SPI/GEG complexes, respectively. Moreover, three charged polysaccharides could all significantly improve the foam stability of complexes. Among them, foams stabilized by SPI/GEG10 were the most stable that the foam volume slightly changed (approximately 1 mL) and no drainage occurred throughout the whole recording process. The interfacial behavior analysis showed that SPI/CS10 had higher diffusion (Kdiff) and rearrangement rate (KR) but lower penetration rate (KP) at the A/W interface compared with single SPI, while SPI/GUG10 and all SPI/GEG complexes showed higher KR and KP but lower Kdiff. In addition, SPI/CS10 was beneficial to concurrently enhance the elastic strength and solid-like behavior of foam system, while all SPI/GEG complexes could improve the elastic strength of foam system but was not conducive to the solid-like behavior.


Assuntos
Ar , Polissacarídeos , Reologia , Proteínas de Soja , Água , Proteínas de Soja/química , Água/química , Polissacarídeos/química , Gomas Vegetais/química , Galactanos/química , Polissacarídeos Bacterianos/química , Quitosana/química , Adsorção , Mananas/química
14.
Int J Biol Macromol ; 268(Pt 1): 131535, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38631586

RESUMO

Herein, efficient degradation of hexabromocyclododecane (HBCD) and Lindane, a persistent organic pollutant using guar gum based calcium oxide doped silicon dioxide (GG-CaO@SiO2) has been reported. The nanocomposite was prepared by waste egg shell (CaO) and rice husk (SiO2) was well characterized. The maximum degradation of HBCD and Lindane were observed at 8 mg catalyst loading, neutral pH, and 2 mg L-1 of pollutant amount. The photocatalytic performance of GG-CaO@SiO2 for HBCD and Lindane photodegradation was evaluated, and it was found that the rate constant increased in the order of GG-CaO@SiO2 > CaO@SiO2 > GG. The polymeric GG-CaO@SiO2 nanocomposite showed maximum removal of both pollutants due to higher surface area (70 m2 g-1) and synergistic interactions among GG moieties. It achieved HBCD and Lindane elimination rates of 94 % and 90 % by photo-adsorptive degradation within 150 min. Meanwhile, the leaching of HBCD from expanded polystyrene (EPS) materials (0.14 ± 0.05 ppm) underwater with different time intervals and degradation of leachate HBCD were also assessed. The eradication of the pollutant manifested first-order kinetics, with the Langmuir adsorption. LC-MS analysis confirmed that GG-CaO@SiO2 effectively breaks down complex structure toxic pollutants into safer metabolites under natural sunlight exposure. The polymeric GG-CaO@SiO2 nanocomposite showed notable reusability up to ten cycle promotes sustainability.


Assuntos
Galactanos , Hexaclorocicloexano , Mananas , Nanocompostos , Gomas Vegetais , Gomas Vegetais/química , Mananas/química , Nanocompostos/química , Galactanos/química , Hexaclorocicloexano/química , Dióxido de Silício/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/isolamento & purificação , Compostos de Cálcio/química , Óxidos/química , Fotólise , Adsorção , Resíduos , Catálise , Concentração de Íons de Hidrogênio , Hidrocarbonetos Bromados
15.
Int J Biol Macromol ; 268(Pt 1): 131837, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38663707

RESUMO

Delayed wound healing is often caused by bacterial infections and persistent inflammation. Multifunctional materials with anti-bacterial, anti-inflammatory, and hemostatic properties are crucial for accelerated wound healing. In this study, we report a biomacromolecule-based scaffold (ArCh) by uniquely combining arabinogalactan (Ar) and chitosan (Ch) using a Schiff-based reaction. Further, the optimized ArCh scaffolds were loaded with Glycyrrhizin (GA: anti-inflammatory molecule) conjugated NIR light-absorbing Copper sulfide (CuS) nanoparticles. The resultant GACuS ArCh scaffolds were characterized for different wound healing parameters in in-vitro and in-vivo models. Our results indicated that GACuS ArCh scaffolds showed excellent swelling, biodegradation, and biocompatibility in vitro. Further results obtained indicated that GACuS ArCh scaffolds demonstrated mild hyperthermia and enhanced hemostatic, anti-oxidant, anti-bacterial, and wound-healing effects when exposed to NIR light. The scaffolds, upon further validation, may be beneficial in accelerating wound healing and tissue regeneration response.


Assuntos
Materiais Biocompatíveis , Quitosana , Galactanos , Alicerces Teciduais , Cicatrização , Quitosana/química , Quitosana/farmacologia , Cicatrização/efeitos dos fármacos , Alicerces Teciduais/química , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Galactanos/química , Galactanos/farmacologia , Regeneração/efeitos dos fármacos , Camundongos , Antibacterianos/farmacologia , Antibacterianos/química , Ratos , Humanos
16.
Int J Biol Macromol ; 268(Pt 1): 131593, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38631571

RESUMO

Asparagus officinalis L. is a horticultural crop that contains a variety of bioactive compounds with anti-inflammatory effects. Aqueous extracts of A. officinalis can noticeably improve the learning and memory function of model mice. Herein, a pectin-arabinoglucuronogalactan complex (AOPB-1-1) with a relative molecular weight of 90.8 kDa was isolated from A. officinalis. The repeating structural unit of AOPB-1-1 was identified through monosaccharide composition, methylation analysis, uronic acid reduction, partial acid hydrolysis, and nuclear magnetic resonance spectroscopy. AOPB-1-1 contains the rhamnogalacturonan-I (RG-I) domain of pectin polysaccharides (PPs) and arabinoglucuronogalactan (AGG) regions. The backbone of the AGG region is composed of →3,6)-ß-D-Galp-(1→ and →4)-ß-D-Glcp-(1→ residues substituted at the 4-position to the →4)-α-D-GalAp-(1→ residues of the RG-I main chain. The anti-neuroinflammatory activity of AOPB-1-1 suggests that it can significantly reduce the content of inflammatory cytokines, including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and inhibit the expression of inflammatory genes including cyclooxygenase-2 (COX2), nitric oxide synthase (iNOS), TNF-α, IL-6, and interleukin-1ß (IL-1ß) in LPS-stimulated BV2 cells. Furthermore, its inhibitory effects on TNF-α and IL-6 levels were even better than those of minocycline. The significant anti-neuroinflammatory activity of AOPB-1-1 suggests its applicability as a therapeutic option for the treatment of Alzheimer's disease.


Assuntos
Anti-Inflamatórios , Asparagus , Pectinas , Pectinas/farmacologia , Pectinas/química , Pectinas/isolamento & purificação , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/química , Asparagus/química , Animais , Camundongos , Galactanos/farmacologia , Galactanos/química , Galactanos/isolamento & purificação , Citocinas/metabolismo , Óxido Nítrico/metabolismo , Peso Molecular
17.
Int J Biol Macromol ; 267(Pt 2): 131521, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38608976

RESUMO

Herein, the effects of anionic xanthan gum (XG), neutral guar gum (GG), and neutral konjac glucomannan (KGM) on the dissolution, physicochemical properties, and emulsion stabilization ability of soy protein isolate (SPI)-polysaccharide conjugates were studied. The SPI-polysaccharide conjugates had better water dissolution than the insoluble SPI. Compared with SPI, SPI-polysaccharide conjugates had lower ß-sheet (39.6 %-56.4 % vs. 47.3 %) and α-helix (13.0 %-13.2 % vs. 22.6 %) percentages, and higher ß-turn (23.8 %-26.5 % vs. 11.0 %) percentages. The creaming stability of SPI-polysaccharide conjugate-stabilized fish oil-loaded emulsions mainly depended on polysaccharide type: SPI-XG (Creaming index: 0) > SPI-GG (Creaming index: 8.1 %-21.2 %) > SPI-KGM (18.1 %-40.4 %). In addition, it also depended on the SPI preparation concentrations, glycation times, and glycation pH. The modification by anionic XG induced no obvious emulsion creaming even after 14-day storage, which suggested that anionic polysaccharide might be the best polysaccharide to modify SPI for emulsion stabilization. This work provided useful information to modify insoluble proteins by polysaccharides for potential application.


Assuntos
Emulsões , Óleos de Peixe , Galactanos , Mananas , Gomas Vegetais , Polissacarídeos Bacterianos , Solubilidade , Proteínas de Soja , Mananas/química , Polissacarídeos Bacterianos/química , Gomas Vegetais/química , Emulsões/química , Proteínas de Soja/química , Galactanos/química , Óleos de Peixe/química , Ânions/química
18.
Int J Biol Macromol ; 267(Pt 2): 131586, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38615861

RESUMO

While hydrogels have potential for food packaging, limited research on hydrogels with excellent mechanical performance and antibacterial activity for preserving chicken breasts. Herein, we created antibacterial hydrogels by embedding methyl-ß-cyclodextrin/thyme oil inclusion complexes (MCD/TO-ICs) into a polyvinyl alcohol matrix containing dendrobium polysaccharides and guar gum in varying ratios using freeze-thaw cycling method. The resulting hydrogels exhibited a more compact structure than those without MCD/TO-ICs, enhancing thermal stability and increasing glass transition temperature due to additional intermolecular interactions between polymer chains that inhibited chain movement. XRD analysis showed no significant changes in crystalline phase, enabling formation of a 3D network through abundant hydrogen bonding. Moreover, the hydrogel demonstrated exceptional durability, with a toughness of 350 ± 25 kJ/m3 and adequate tearing resistance of 340 ± 30 J/m2, capable of lifting 3 kg weight, 1200 times greater than the hydrogel itself. Additionally, the hydrogels displayed excellent antimicrobial activity and antioxidant properties. Importantly, the hydrogels effectively maintained TVB-N levels and microbial counts within acceptable ranges, preserving sensory properties and extending the shelf life of chilled chicken breasts by four days. This study highlights the potential of MCD/TO-IC-incorporated polysaccharide hydrogels as safe and effective active packaging solutions for preserving chilled chicken in food industry.


Assuntos
Galinhas , Hidrogéis , Polissacarídeos , Animais , Hidrogéis/química , Hidrogéis/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Conservação de Alimentos/métodos , beta-Ciclodextrinas/química , Embalagem de Alimentos/métodos , Galactanos/química , Galactanos/farmacologia , Anti-Infecciosos/farmacologia , Anti-Infecciosos/química , Antibacterianos/farmacologia , Antibacterianos/química , Antioxidantes/farmacologia , Antioxidantes/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Mananas , Gomas Vegetais
19.
Int J Biol Macromol ; 267(Pt 2): 131591, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38621574

RESUMO

In the present study, a novel environment friendly dry method for preparation of guar gum maleate (GGM) with varying degrees of substitution (DS; 0.02-1.04) was optimized. GGM with a maximum DS of 1.04 was successfully synthesized using guar gum (GG) and maleic anhydride (MA) in proportion of 1: 1 at 80 °C with 4 h of reaction time. The activation energy for the reaction was determined to be 36.91 ± 3.61 kJ mol-1 with pre-exponential factor of 1392 min-1. Esterification of GG was confirmed by FT-IR and 13C NMR. Analysis using size exclusion chromatography (SEC) indicated a decrease in weight average molecular weight (Mw) of the polymer with an increase in polydispersity index (PDI) due to esterification. In comparison with GG, GGM displayed increased hydrophobicity and reduced thermal stability, as analysed by differential scanning calorimetry (DSC). Rheological studies of GGM revealed that initial apparent viscosity decreased with increasing DS. For the first time, the study offered valuable insights on GGM synthesis under dry solvent-less reaction conditions enabling simpler and scalable synthesis process.


Assuntos
Galactanos , Maleatos , Mananas , Gomas Vegetais , Gomas Vegetais/química , Galactanos/química , Mananas/química , Cinética , Maleatos/química , Peso Molecular , Viscosidade , Esterificação , Reologia , Temperatura , Técnicas de Química Sintética , Interações Hidrofóbicas e Hidrofílicas
20.
Plant Physiol Biochem ; 210: 108589, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38593485

RESUMO

Carotenoids are important pigmented nutrients synthesized by tomato fruits during ripening. To reveal the molecular mechanism underlying carotenoid synthesis during tomato fruit ripening, we analyzed carotenoid metabolites and transcriptomes in six development stages of tomato fruits. A total of thirty different carotenoids were detected and quantified in tomato fruits from 10 to 60 DPA. Based on differential gene expression profiles and WGCNA, we explored several genes that were highly significant and negatively correlated with lycopene, all of which encode fasciclin-like arabinogalactan proteins (FLAs). The FLAs are involved in plant signal transduction, however the functional role of these proteins has not been studied in tomato. Genome-wide analysis revealed that cultivated and wild tomato species contained 18 to 22 FLA family members, clustered into four groups, and mainly evolved by means of segmental duplication. The functional characterization of FLAs showed that silencing of SlFLA1, 5, and 13 were found to contribute to the early coloration of tomato fruits, and the expression of carotenoid synthesis-related genes was up-regulated in fruits that changed phenotypically, especially in SlFLA13-silenced plants. Furthermore, the content of multiple carotenoids (including (E/Z)-phytoene, lycopene, γ-carotene, and α-carotene) was significantly increased in SlFLA13-silenced fruits, suggesting that SlFLA13 has a potential inhibitory function in regulating carotenoid synthesis in tomato fruits. The results of the present study broaden the idea of analyzing the biological functions of tomato FLAs and preliminary evidence for the inhibitory role of SlFLA13 in carotenoid synthesis in fruit, providing the theoretical basis and a candidate for improving tomato fruit quality.


Assuntos
Carotenoides , Frutas , Proteínas de Plantas , Solanum lycopersicum , Solanum lycopersicum/genética , Solanum lycopersicum/metabolismo , Solanum lycopersicum/crescimento & desenvolvimento , Carotenoides/metabolismo , Frutas/metabolismo , Frutas/genética , Frutas/crescimento & desenvolvimento , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Regulação da Expressão Gênica de Plantas , Galactanos/metabolismo , Galactanos/biossíntese , Mucoproteínas/metabolismo , Mucoproteínas/genética
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