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1.
Clin Nucl Med ; 47(2): 190-191, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-35006111

RESUMO

ABSTRACT: A 7-year-old boy with Wiskott-Aldrich syndrome underwent FDG PET/CT to evaluate hepatic lesions revealed by an ultrasound. The images demonstrated multiple hypermetabolic lesions in the liver, lung, and lymph nodes. Biopsy of liver and lymph nodes demonstrated Epstein-Barr virus-associated leiomyosarcoma.


Assuntos
Infecções por Vírus Epstein-Barr , Leiomiossarcoma , Síndrome de Wiskott-Aldrich , Criança , Fluordesoxiglucose F18 , Herpesvirus Humano 4 , Humanos , Masculino , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada
3.
Magn Reson Imaging Clin N Am ; 30(1): 19-33, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34802578

RESUMO

Nasopharyngeal carcinoma is endemic in parts of the world such as southern China and Southeast Asia. It is predominantly an undifferentiated carcinoma with a strong genetic basis and a close association with the Epstein-Barr virus. The ability of MR imaging to depict the boundaries of the primary tumor and its relationship with the complex structures of the skull base makes it the technique of choice for imaging of this disease in the head and neck. This article describes the MR imaging findings pertinent to staging and management and a new role of MR imaging in early cancer detection, in addition to a brief discussion of differential diagnoses.


Assuntos
Infecções por Vírus Epstein-Barr , Neoplasias Nasofaríngeas , Herpesvirus Humano 4 , Humanos , Imageamento por Ressonância Magnética , Carcinoma Nasofaríngeo/diagnóstico por imagem , Neoplasias Nasofaríngeas/diagnóstico por imagem
4.
Anal Chim Acta ; 1189: 339210, 2022 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-34815051

RESUMO

Circular Ribonucleic Acid (CircRNA) plays regulatory roles in many biological processes, such as tumors and metabolic diseases. Due to the fact that circRNA is more stable and conservative than linear RNA, circRNA has become a potential biomarker in early clinical diagnosis and biomedical research. Therefore, the quantification of circRNA expression level is of importance for understanding their functions and their applications for disease diagnosis and treatment. Nevertheless, due to the low abundance of circRNA, it is still a challenge for the analysis of circRNA in cells. Herein, we proposed a sensitive detection method for circRNA based on the T7 exonuclease-assisted cycling enzymatic amplification. The fluorescent sensor was constructed by a hairpin molecular beacon and T7 exonuclease. With the cycling enzymatic amplification process, this sensor achieved the limit of detection of 1 pM with a good linear correlation in the range of 0-100 pM (R2 = 0.9891) using circBART2.2 as a model. Furthermore, we applied the proposed method in the determination of circBART2.2 in cell lysates. The results demonstrated that this method has promising applications in early diagnosis of Epstein-Barr virus (EBV) infection-related diseases using circRNA as the biomarker.


Assuntos
Infecções por Vírus Epstein-Barr , RNA Circular , Contagem de Células , Herpesvirus Humano 4 , Humanos , Limite de Detecção , Espectrometria de Fluorescência
5.
Int J Cancer ; 150(2): 327-334, 2022 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-34520566

RESUMO

Elevated Epstein-Barr virus (EBV) DNA load is common in lymphomas. However, it remains unclear whether the disparity in viral load and its prognostic value in lymphomas are correlated with Epstein-Barr encoding region (EBER) status. In this retrospective multicenter study, we collected the data of pretreatment whole blood EBV DNA (pre-EBV DNA) and EBER status and evaluated their disparity and prognostic values in lymphomas. A total of 454 lymphoma patients from December 2014 to August 2020 were retrospectively retrieved. Mann-Whitney U test, Kruskal-Wallis test and Bonferroni's adjustment were used to explore the disparity of EBV DNA and EBER status in lymphomas. Time-dependent receiver operating characteristic analysis and MaxStat analysis were used to determine optimal cutoff points of pre-EBV DNA load. Univariable and multivariable Cox proportional hazards models were established for the estimation of prognostic factors. The positive rate of EBV DNA in natural killer T-cell lymphoma (NKTL) patients was higher than that in diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL) and Hodgkin lymphoma (HL) patients, and the median positive pre-EBV copy number of NKTL was also higher than that of FL and DLBCL. EBV DNA could clearly distinguish the prognosis of DLBCL, NKTL, HL and peripheral T-cell lymphoma, and the integration of EBER status and EBV DNA could differentiate the prognosis of HL patients. Multivariable results revealed that pre-EBV DNA load had an effect on the prognosis of NKTL, FL and DLBCL. The status of pre-EBV DNA and EBER were disparate. Whole blood pre-EBV DNA predicted the prognosis of lymphomas, and the combination of EBV and EBER status could differentiate the prognosis of HL.


Assuntos
DNA Viral/genética , Infecções por Vírus Epstein-Barr/complicações , Herpesvirus Humano 4/genética , Doença de Hodgkin/diagnóstico , Linfoma Folicular/diagnóstico , Linfoma Difuso de Grandes Células B/diagnóstico , Linfoma de Células T/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , DNA Viral/análise , Diagnóstico Diferencial , Infecções por Vírus Epstein-Barr/virologia , Feminino , Seguimentos , Herpesvirus Humano 4/isolamento & purificação , Doença de Hodgkin/epidemiologia , Doença de Hodgkin/virologia , Humanos , Linfoma Folicular/epidemiologia , Linfoma Folicular/virologia , Linfoma Difuso de Grandes Células B/epidemiologia , Linfoma Difuso de Grandes Células B/virologia , Linfoma de Células T/epidemiologia , Linfoma de Células T/virologia , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Taxa de Sobrevida
6.
Biosens Bioelectron ; 195: 113672, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34601264

RESUMO

We present the first combination of a microfluidic polymerase chain reaction (PCR) with a gold nanoslit-based surface plasmon resonance (SPR) sensor for detecting the DNA sequence of latent membrane protein 1 (LMP1). The PCR microchannel was produced through a laser scribing technique, and the SPR nanoslit chip was manufactured via hot-embossing nanoimprinting lithography. Afterward, the LMP1 DNA probe was adsorbed onto the SPR chip of the integrated device through electrostatic interactions for further detection. The device can complete the analytical procedure in around 36 min, while the traditional machine requires 105 min to achieve similar signals under the same PCR thermal cycles. The calibration curve with serially diluted LMP1 DNA exhibited the accuracy (R2 > 0.99) and sensitivity (limit of detection: ∼10-11 g/mL) of the device. Moreover, extracted DNA from Epstein-Barr virus (EBV)-positive cells were directly detected through the integrated chip. In brief, this all-in-one chip can amplify gene fragments at the front-end and detect them at the back-end, decreasing the time required for the analysis without compromising accuracy or sensitivity. We believe this label-free, real-time, low-cost device has enormous potential for rapid detection of various viruses, such as EBV and COVID-19.


Assuntos
Técnicas Biossensoriais , COVID-19 , Infecções por Vírus Epstein-Barr , Ouro , Herpesvirus Humano 4/genética , Humanos , Microfluídica , Reação em Cadeia da Polimerase , SARS-CoV-2 , Proteínas da Matriz Viral/genética
7.
J Oncol Pharm Pract ; 28(1): 222-227, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34074166

RESUMO

INTRODUCTION: Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening syndrome characterized by excessive production of inflammatory cytokines and multiple organs injury. Ruxolitinib, an oral selective JAK1/2 inhibitor, has recently shown efficacy and safety in the treatment of secondary HLH, which may be an alternative to intensive chemotherapy. CASE REPORT: We report a case of a 2-year-old boy who presented to our institution with recurrent fever and acute renal failure. We made the diagnosis of Epstein-Barr virus related HLH based on the HLH-2004 protocol, and gave the treatment of ruxolitinib instead of etoposide. MANAGEMENT AND OUTCOME: The patient received dexamethasone and continuous renal replacement therapy due to renal failure, but he still had fever and anuria. Given that the use of etoposide may deteriorate renal function, ruxolitinib was administered instead of etoposide. After 5 days of ruxolitinib treatment, the patient's fever was resolved and renal function also gradually recovered 14 days later. DISCUSSION: Currently, dexamethasone, etoposide and cyclosporine A are the main drugs in HLH treatment. However, cytotoxic chemotherapy can temporally deteriorate organ damage and induce serious myelosuppression, which makes clinicians hesitate to implement these regimens. Ruxolitinib has shown efficacy in treating HLH without much toxicity in clinical trials. Thus, we suggest that ruxolitinib constitutes a treatment option for secondary HLH complicated by severe renal damage which may reduce toxic effects compared with intense chemotherapy.


Assuntos
Injúria Renal Aguda , Infecções por Vírus Epstein-Barr , Linfo-Histiocitose Hemofagocítica , Injúria Renal Aguda/induzido quimicamente , Injúria Renal Aguda/tratamento farmacológico , Pré-Escolar , Etoposídeo/uso terapêutico , Herpesvirus Humano 4 , Humanos , Linfo-Histiocitose Hemofagocítica/tratamento farmacológico , Masculino , Nitrilas , Pirazóis , Pirimidinas
8.
Clin Nucl Med ; 47(1): e105-e107, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34269731

RESUMO

ABSTRACT: Epstein-Barr virus-positive mucocutaneous ulcer is a newly recognized clinicopathological entity among mature B-cell neoplasms according to the 2016 revision of the World Health Organization diagnostic criteria. Here, we present FDG PET/CT images of 2 Epstein-Barr virus-positive mucocutaneous ulcer cases. Both cases shown in the images mimicked head and neck cancers, which are similar to carcinomas of the tonsil and gingiva, respectively, and both lesions showed intense FDG uptake on PET scan.


Assuntos
Infecções por Vírus Epstein-Barr , Neoplasias de Cabeça e Pescoço , Infecções por Vírus Epstein-Barr/diagnóstico por imagem , Fluordesoxiglucose F18 , Neoplasias de Cabeça e Pescoço/diagnóstico por imagem , Herpesvirus Humano 4 , Humanos , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Tomografia por Emissão de Pósitrons , Úlcera/diagnóstico por imagem
9.
Anticancer Res ; 42(1): 555-563, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34969765

RESUMO

BACKGROUND: Epstein-Barr virus (EBV)-associated gastric cancer has been identified as a cancer subtype with definitive clinical and molecular characteristics. Although olaparib, a poly ADP ribose polymerase (PARP) inhibitor, is considered a potential effective agent for gastric cancer, the effect and underlying mechanism of olaparib on gastric cancer depending on EBV infection is not fully understood. MATERIALS AND METHODS: EBV-positive SNU719 and EBV-negative SNU638 gastric cancer cell lines were used to identify the effects of olaparib using the trypan blue exclusion method and annexin V staining assay. To observe the underlying cellular signaling mechanisms of olaparib-induced cell death, Epstein-Barr virus nuclear antigen 1 (EBNA1) and signaling related molecule expression were assessed using transfection, silencing of specific genes using small interfering RNA (siRNA), western blotting and signaling inhibition assay. RESULTS: Olaparib decreased the cell viability of EBV-positive SNU719 gastric cancer cells through caspase-3-dependent apoptosis in a dose dependent manner, whereas EBV-negative SNU638 gastric cancer cells showed drug resistance to olaparib. EBNA1 was expressed in SUN719 gastric cancer cells; however, ataxia telangiectasia and Rad3 related (ATR) and phosphorylated ATR kinase were expressed in SNU638 gastric cancer cells. EBNA1 transfection decreased ATR phosphorylation through p38 mitogen-activated protein kinase (MAPK) phosphorylation in SUN638 gastric cancer cells, and silencing of ATR kinase increased the susceptibility of these cells to olaparib treatment. Moreover, VE-821, an ATR kinase specific inhibitor, also increased the sensitivity of SNU638 cells to olaparib. In contrast, SB203580, a p38 MAPK inhibitor, inhibited this increase in sensitivity to olaparib by EBNA1 transfection. CONCLUSION: Olaparib treatment led to different cellular responses depending on EBV infection in gastric cancer cell lines. These results provide new insights into the mechanism of olaparib-induced apoptosis in gastric cancer cells and suggest that EBV infection should be considered when developing new potential therapeutic agents for gastric cancer.


Assuntos
Proteínas Mutadas de Ataxia Telangiectasia/genética , Antígenos Nucleares do Vírus Epstein-Barr/genética , Ftalazinas/farmacologia , Piperazinas/farmacologia , Neoplasias Gástricas/tratamento farmacológico , Proteínas Quinases p38 Ativadas por Mitógeno/genética , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Infecções por Vírus Epstein-Barr/tratamento farmacológico , Infecções por Vírus Epstein-Barr/genética , Infecções por Vírus Epstein-Barr/patologia , Infecções por Vírus Epstein-Barr/virologia , Antígenos Nucleares do Vírus Epstein-Barr/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Herpesvirus Humano 4/isolamento & purificação , Herpesvirus Humano 4/patogenicidade , Humanos , Transdução de Sinais/efeitos dos fármacos , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Neoplasias Gástricas/virologia , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores
10.
Methods Mol Biol ; 2383: 275-291, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34766297

RESUMO

Transcription factors (TFs) are key players in the control of gene expression and consequently all major cellular process, ranging from cell fate determination to cell cycle control and response to the environment.In particular cases, their ectopic expression has shown great promise in cell reprogramming for regenerative medicine, ontogenesis studies, and cell modeling. The current reprogramming methods mainly rely on gene transfer, therefore require technological improvements to limit genetic imprinting and improve safety. Direct protein delivery could represent an attractive alternative. Cell-penetrating peptides (CPPs) fused to recombinant TFs or other proteins involved in the epigenetic definition of cells have great potential in this context. We have thus developed the direct vectorization of Oct4, Sox2, or Nanog TFs and the posttranscriptional regulatory RNA-binding protein Lin28a by using the minimal transduction domain (MD11) of Epstein-Barr virus ZEBRA protein.This section describes the molecular cloning and production of different TFs fused to ZEBRA MD11 domain in the E. coli expression system. We also include the optimized purification conditions for each recombinant protein. The treatment of primary fibroblasts as well as cord blood-derived hematopoietic stem cells is also described. Finally, the transcriptional activation of the target genes following the transfer of TFs analyzed by quantitative PCR is presented.Our work primarily finds applications for advanced medicinal products, an area that requires novel therapy designs and delivery systems devoid of genetic material transfer to improve safety.


Assuntos
Transcrição Genética , Animais , Reprogramação Celular/genética , Infecções por Vírus Epstein-Barr , Equidae , Escherichia coli , Herpesvirus Humano 4 , RNA Mensageiro/genética , Fatores de Transcrição/genética
11.
Oncol Rep ; 47(1)2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34779497

RESUMO

Epstein­Barr virus (EBV) is endemic worldwide and is associated with a number of human tumors. EBV­associated tumors have unique mechanisms of tumorigenesis. EBV encodes multiple oncogenic molecules that can be loaded into exosomes released by EBV+ tumor cells to mediate intercellular communication. Moreover, different EBV+ tumor cells secrete exosomes that act on various target cells with various biological functions. In addition to oncogenicity, EBV+ exosomes have potential immunosuppressive effects. Investigating EBV+ exosomes could identify the role of EBV in tumorigenesis and progression. The present review summarized advances in studies focusing on exosomes and the functions of EBV+ exosomes derived from different EBV­associated tumors. EBV+ exosomes are expected to become a new biomarker for disease diagnosis and prognosis. Therefore, exosome­targeted therapy displays potential.


Assuntos
Carcinoma/patologia , Carcinoma/virologia , Infecções por Vírus Epstein-Barr/patologia , Infecções por Vírus Epstein-Barr/virologia , Exossomos/patologia , Exossomos/virologia , Herpesvirus Humano 4 , Humanos
12.
Biosens Bioelectron ; 199: 113873, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34953301

RESUMO

For digital polymerase chain reaction (PCR), data classification is always a crucial task. The dynamic real-time amplification process information of each partition is always ignored in typical digital PCR analysis, which can easily lead to inaccurate outcomes. In this work, an integrated device that offers real-time chip-based digital PCR analysis was established. In addition, an enhanced process-based classification model (PAM) was built and trained. And then the device and the analytical model were employed in classification tasks for different concentrations of Epstein-Barr Virus (EBV) plasmid quantification assays. The results indicated that the real-time analysis device achieved a linearity of 0.97, the classification method was able to distinguish the false-positive curves, and the recognition error of positive wells was decreased by 64.4% compared with typical static analysis techniques when low concentrations of samples were tested. With these advantages, it is supposed that the real-time digital PCR analysis apparatus and the improved classification method can be employed to enhance the performance of digital PCR technology.


Assuntos
Técnicas Biossensoriais , Infecções por Vírus Epstein-Barr , Herpesvirus Humano 4/genética , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Tecnologia
13.
Stomatologiia (Mosk) ; 100(6): 19-23, 2021.
Artigo em Russo | MEDLINE | ID: mdl-34953183

RESUMO

OBJECTIVE: The aim of the study was to study the microflora of periodontal pockets and gingival fluid; determine the qualitative composition of periodontal pathogens by PCR; to determine the main associations of microorganisms, to determine the periodontal index in patients with cement caries, external pathological resorption and in patients from the comparison group. MATERIAL AND METHODS: The examination included 80 patients, including 28 patients with a diagnosis of cement caries, 25 patients with a diagnosis of external cervical root resorption, 27 patients in the comparison group without tooth root damage. Based on the results of microbiological studies in various clinical conditions of the oral cavity, in particular, in different forms of lesions of the periodontium and hard tissues of teeth, the predominant microbial representatives and their combinations were identified. RESULTS: According to the study, it was revealed that all patients with cement caries and external pathological resorption have pathological processes arising in the periodontium. The study of the qualitative composition of the microflora showed that in patients with cement caries, the following associations of microorganisms are most often found: Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Tannerella forsythia, Porphyromonas gingivalis, Candida albicans, Treponema denticola. Patients with external pathological resorption of microorganisms were found in patients with external pathological resorption of microorganisms: Prevotella intermedia, Treponema denticola, Candida albicans, Tannerella forsythia, Porphyromonas gingivalis, Epstein-Barr virus. These periodontal pathogenic microorganisms have a wide range of pathogenicity factors, which allows them to induce a prolonged inflammatory process. CONCLUSION: The study of the microflora of periodontal pockets and gingival sulcus in case of caries of cement and external pathological resorption is an important link in preventing the development and aggravation of pathological conditions of hard tissues of the tooth root and periodontal tissues.


Assuntos
Infecções por Vírus Epstein-Barr , Cárie Radicular , Aggregatibacter actinomycetemcomitans , Herpesvirus Humano 4 , Humanos , Boca , Reação em Cadeia da Polimerase , Porphyromonas gingivalis , Prevotella intermedia , Treponema denticola
14.
Korean J Gastroenterol ; 78(6): 349-352, 2021 12 25.
Artigo em Inglês | MEDLINE | ID: mdl-34955512

RESUMO

Natural killer (NK) cell enteropathy is not well understood. Although NK-cell enteropathy is typically benign, aggressive NK-cell lymphoma is an important differential diagnosis. This paper reports a rare case of NK-cell enteropathy of the stomach presenting with dyspepsia and weight loss. In this case, upper endoscopy revealed an ulcer-like lesion (approximately 7 mm) surrounded by erythema, edema, and mucosal hemorrhage. The biopsy revealed atypical lymphocyte cells expressing CD56 without an Epstein-Barr virus infection, which is consistent with NK-cell enteropathy. Follow-up endoscopy 2 months later revealed lesion regression with mild erythema. The patient was observed closely for 6 months, with no evidence of lymphoma.


Assuntos
Infecções por Vírus Epstein-Barr , Transtornos Linfoproliferativos , Idoso , Feminino , Herpesvirus Humano 4 , Humanos , Células Matadoras Naturais , Estômago
15.
J Int Med Res ; 49(12): 3000605211063292, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34918995

RESUMO

Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) produces similar survival outcomes as HLA-matched sibling donor allogeneic HCST in younger patients with acquired severe aplastic anemia (SAA). This study reported a 29-years-old man with SAA and intracranial hemorrhage who underwent haplo-HSCT with a modified BU/CY + ATG conditioning regimen. Neutrophil and platelet engraftment were both achieved on day 14 after HSCT. The patient developed grade IV acute graft-versus-host disease (aGVHD) on day 20 and acquired cytomegalovirus (CMV) and Epstein-Barr virus (EBV) infections on day 47. After the failure of methylprednisolone, basiliximab, ruxolitinib, and antiviral treatment, the patient was diagnosed with steroid-resistant grade IV aGVHD and refractory CMV and EBV infections. We performed fecal microbiota transplantation and infused CMV- and EBV-specific cytotoxic T lymphocytes. After that the stool volume and frequency gradually decreased, and viral DNA was undetectable on day 80. This report provides helpful clinical experience for treating steroid-resistant aGVHD and refractory viral infections.


Assuntos
Infecções por Vírus Epstein-Barr , Doença Enxerto-Hospedeiro , Transplante de Células-Tronco Hematopoéticas , Adulto , Citomegalovirus , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/terapia , Transplante de Microbiota Fecal , Doença Enxerto-Hospedeiro/tratamento farmacológico , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Herpesvirus Humano 4 , Humanos , Esteroides/uso terapêutico
17.
PLoS One ; 16(12): e0261665, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34937060

RESUMO

BACKGROUND: The risk of infectious mononucleosis (IM) is affected both by crowding and by sibship structure, i.e., number and signed age differential between an index child and a sibling. Siblings provide protection against IM by pre-empting delayed primary Epstein-Barr virus infection with its associated high risk of IM. The association between childcare attendance and risk of IM, on the other hand, has never been studied in a large, well-characterized cohort. METHODS: Danish children born in July 1992 through 2016 with a completely known simple childcare attendance history before age 1.5 years (n = 908,866) were followed up for a hospital contact with an IM diagnosis at ages 1.5-26 years. Hazard ratios (HRs) of IM for an additional year of exposure were obtained from stratified Cox regression analyses, stratified by sex and year of birth, with age as the underlying time scale, adjusted for sibship structure, and sociodemographic variables including parental ethnicity and maternal age. RESULTS: An additional year of exclusively attending a daycare home (max 5 children) yielded HR = 0.90 (95% confidence interval 0.81-1.00), and similarly, each year of exclusively attending a childcare institution (e.g., crèche) yielded HR = 0.94 (0.84-1.06). CONCLUSIONS: Forwarding enrollment in childcare by a year lowers the risk of IM later in life much less than having an additional sibling of comparable age and has no practical public health implications. We find our results suggestive of a random threshold for successful Epstein-Barr virus infection that is more easily reached by a sibling than the collective of playmates in daycare homes or childcare institutions.


Assuntos
Cuidado da Criança , Mononucleose Infecciosa/etiologia , Adolescente , Adulto , Criança , Saúde da Criança , Pré-Escolar , Estudos de Coortes , Dinamarca/epidemiologia , Feminino , Herpesvirus Humano 4/isolamento & purificação , Humanos , Lactente , Mononucleose Infecciosa/diagnóstico , Masculino , Fatores de Risco , Irmãos , Adulto Jovem
18.
New Microbiol ; 44(4): 217-226, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34942011

RESUMO

We aimed to detect EBV/Hp (Epstein-Barr Virus/Helicobacter pylori) co-infection by determining the number of copies of EBV/EBER-1 in the gastric biopsy samples of the Hp (+) GC, peptic ulcer (PU), and non-ulcer dyspepsia (NUD) cases. The patient group (PG), with 34 patients (34 GC and 30 PU patients) and a control group with 40 NUD cases were included. All patients and controls were Hp positive. EBV/EBNA-1 IgG were measured by the Anti-EBNA-1 ELISA IgG kit. Determination and quantification of EBV/EBER-1 gene region was performed by qPCR. EBV/EBER-1 positivity was 35.29% (12/34), 6.6% (2/30) and 2.5% (1/40) in GC, PU and 40 NUD cases, respectively. A significant difference was found between the GC and NUD cases (p=0.001). A significant difference was found between the groups for mean EBV/EBER-1 copy numbers (p=0.019). No significant difference was found between GC and the NUD cases (p=0.1455) for EBV/EBNA-1 IgG antibody positivity. EBV/EBER-1 positivity (OR=3.319), and age ≥55 years old (OR=2.331) were found to be a significant in multivariate logistic regression. In conclusion, our data suggest that the GC risk by EBVand Hp co-infection increased 3.3 times.


Assuntos
Coinfecção , Infecções por Vírus Epstein-Barr , Helicobacter pylori , Neoplasias Gástricas , Herpesvirus Humano 4 , Humanos , Pessoa de Meia-Idade , Úlcera
19.
Blood ; 138(26): 2743-2744, 2021 12 30.
Artigo em Inglês | MEDLINE | ID: mdl-34967867
20.
Acta Gastroenterol Belg ; 84(4): 663-665, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34965049

RESUMO

Plasmablastic lymphoma (PBL) represents a rare and aggressive subtype of diffuse large B cells lymphoma (DLBCL) most associated with the human immunodeficiency virus (HIV). Prognosis remains poor despite various treatment approaches. We describe an evolution at six months of HIV negative PBL and Ebstein Barr virus (EBV) positive PBL with chemotherapy. Role of radiotherapy is still unclear.


Assuntos
Infecções por Vírus Epstein-Barr , Linfoma Plasmablástico , Neoplasias da Próstata , HIV , Herpesvirus Humano 4 , Humanos , Masculino , Linfoma Plasmablástico/diagnóstico , Linfoma Plasmablástico/etiologia
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