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1.
Braz. j. biol ; 84: e253084, 2024. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1345551

RESUMO

Abstract Repeatedly frying process of dietary edible oil has a potential role in the generation of free radicals. Therefore, questions have always been raised as to whether, there is an efficient and economical method to reduce the harmful effects of repeated use of frying edible oil. Since hibiscus has been stated to have a wide variety of therapeutic effects, it was important to investigate its properties against harmful effects of free radicals. The current study aspires to find out whether irradiated powder of hibiscus has a protective role against adverse effects of repeated use of frying edible oil. Thirty-five adult male albino rats were equally assigned into five groups. First group"G1" was fed with normal diet as control group, meanwhile, group"G2" the diet mixed with fresh oil, "G3" diet mixed with repeatedly frying oil only, "G4" diet mixed with frying oil treated with hibiscus and "G5" diet mixed with frying oil treated with irradiated hibiscus. Feeding duration was six weeks. Fatty acid analyses of oil as well as peroxide values were determined. Blood and liver samples were collected for biochemical analyses as well as histological study. Repeatedly heated cooked oil has significant increases in peroxide value, acid value, free fatty acid and both conjugated diene and triene compared with repeatedly frying oil treated with hibiscus. Also there are significant increases in cholesterol and triglyceride and impaired in liver functions in "G3"compared with others. In addition, relative to the hibiscus groups, there is a substantial reduction in oxygen consumption in "G3". Both hibiscus as well as irradiated hibiscus attract attention in order to play a vital and economical role against harmful effects of frequent use of frying edible oil on some biological functions but, irradiated hibiscus was more effective.


Resumo O processo de fritura repetida de óleo comestível da dieta tem papel potencial na geração de radicais livres que podem ter efeitos prejudiciais em algumas funções biológicas. Portanto, sempre se questionou se existe uma maneira eficiente e econômica de prevenir ou pelo menos reduzir os efeitos nocivos do uso repetido de óleo comestível para fritar. Como o hibisco tem ampla variedade de efeitos terapêuticos, foi importante investigar suas propriedades como agente antioxidante contra os efeitos nocivos dos radicais livres. O presente estudo pretende descobrir se o pó irradiado de hibisco tem papel protetor contra os efeitos adversos do uso repetido de óleo comestível para fritar. Trinta e cinco ratos albinos machos adultos foram divididos igualmente em cinco grupos. O primeiro grupo "G1" foi alimentado com dieta normal como grupo controle, enquanto o grupo "G2" dieta misturada com óleo fresco, dieta "G3" misturada com óleo de fritura repetida, dieta "G4" misturada com óleo de fritura tratada com hibisco e dieta "G5" misturada com óleo de fritura tratada com hibisco irradiado. A duração da alimentação foi de seis semanas. Foram determinadas as análises de ácidos graxos de óleo, bem como os valores de peróxidos. Amostras de sangue e fígado foram coletadas para análises bioquímicas e estudo histológico. O óleo cozido repetidamente aquecido tem aumentos significativos no valor de peróxido, valor de ácido, ácido graxo livre e dieno e trieno conjugados em comparação com óleo de fritura repetidamente tratado com hibisco. Também há aumentos significativos no colesterol e triglicérides e comprometimento das funções hepáticas no "G3" em comparação com outros. Além disso, em relação aos grupos de hibiscos, há uma redução substancial no consumo de oxigênio no "G3". Tanto o hibisco como o hibisco irradiado chamam atenção por desempenhar papel vital e econômico contra os efeitos nocivos do uso frequente de óleo comestível para fritar em algumas funções biológicas, mas o hibisco irradiado foi mais eficaz.


Assuntos
Animais , Ratos , Hibiscus , Óleos Vegetais/farmacologia , Colesterol , Culinária , Temperatura Alta
2.
BMC Plant Biol ; 22(1): 516, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36333799

RESUMO

BACKGROUND: Data on the microbial community and functional proteins associated with degumming in kenaf remains scant. Here, we analyzed the microbial communities associated with kenaf (Hibiscus cannabinus) bast fibers during retting to identify potential candidate degumming bacteria. Retting liquids were collected and analyzed at 0 days, 10 days, and 34 days and then evaluated the yield and quality of kenaf fiber at the different retting times. Besides, the microbial communities were characterized using metagenomic and proteomic analysis by LC-MS/MS technology. RESULTS: The data showed that increase in the retting time significantly improves the softness, dispersion, and fiber whiteness of the kenaf fiber. The relative abundance of Acinetobacter increased from 2.88% at the baseline to 6.64% at the 34th retting. On the other hand, some members of Clostridium were reduced from 3% at the baseline to 2% at the 34th retting. Analysis of carbohydrate active enzymes showed constant changes in the utilization of carbohydrates. Besides, benzoquinone reductase, cellobiose dehydrogenase, glucose 1-oxidase, aryl alcohol oxidase and alcohol oxidase were the top five most abundant enzymes in the retting liquids. This present results demonstrated that the expressions of B7GYR8, Q6RYW5 and Q6FFK2 proteins were suppressed in Acinetobacter with the retting time. On the contrary, P05149 was upregulated with the retting time. In Clostridium, P37698, P52040 and P54937 proteins were upregulated with the retting time. CONCLUSION: In addition, bacteria Acinetobacter and Clostridium might be playing important roles in the kenaf degumming process. Similarly, up-regulation of P37698, P52040 and P54937 proteins is an important manifestation and mediates important roles in the degumming process.


Assuntos
Hibiscus , Hibiscus/microbiologia , Proteômica , Proteoma , Cromatografia Líquida , Espectrometria de Massas em Tandem , Bactérias
3.
J Med Life ; 15(10): 1246-1251, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36420282

RESUMO

Roselle (Hibiscus sabdariffa L.) belongs to the genus Hibiscus with proven anti-inflammatory, antioxidant, and antimicrobial properties. Scientific evidence associated roselle content with bioactive compounds, such as phenolic acids, flavonoids, and anthocyanin. Most studies focused on their petals, while research on leaf extract on wound healing has never been done. This study aimed to assess the effect of roselle leaf extract on wound healing in rats. This was an experimental laboratory study with a posttest-only control group design. There were 30 rats divided into 5 groups: negative control, 5%, 10%, and 15% roselle leaf extract, and positive control (bioplacenton). The parameters assessed in this research were wound size and histological assessment. The data were analyzed using ANOVA. P<0.05 was considered statistically significant. Wound healing percentage and epithelial thickness in the 15% group were the largest (84.17%; 64.69 µm). The lowest value was recorded in placebo (64%; 36.33 µm). Meanwhile, wound healing percentage and epithelial thickness of rats in the 5% and 10% groups were 68.53%, 43,57 µm, and 78.11%, 56.49 µm, respectively. Finally, positive control had a 77.44% wound healing percentage and 49.7 µm epithelial thickness. There were no significant differences in wound healing and epithelial thickness among the groups. Roselle leaf extract at 15% concentration showed greater wound healing properties based on clinical and histological assessment. Although there were no statistically significant differences, roselle leaf showed an opportunity to be further investigated as a potential wound healing therapy.


Assuntos
Hibiscus , Ratos , Animais , Cicatrização , Antibacterianos , Antioxidantes/farmacologia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico
4.
Oxid Med Cell Longev ; 2022: 4631983, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36187334

RESUMO

Oxidative stress and inflammation play key roles in the pathophysiology in the pathophysiology of dyslipidemia, which are positive risks that increase atherosclerosis leading to important healthcare problems. Therefore, we aimed to study the antioxidant, anti-inflammatory, and lipid-lowering effects of jelly drink containing polyphenol-rich roselle calyces extract and passion fruit juice with pulp concentrate (RP jelly drink) in comparison to a placebo jelly drink for 8 weeks. Forty-three adults with dyslipidemia were randomly assigned into two groups: the RP jelly drink group and the placebo group. Glucose, total cholesterol (TC) triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), oxidative stress biomarkers, inflammatory parameters, and monocyte chemotactic protein-1 (MCP-1) were measured with fasting blood samples at baseline, 4 weeks and 8 weeks of intervention. Results showed a significant decrease in LDL-C and TG, respectively, after 8 weeks of RP jelly drink consumption (LDL-C: 107.63 ± 22.98 mg/dL; TG: 109.79 ± 38.83 mg/dL) compared to baseline measurements (LDL-C: 128.43 ± 32.74 mg/dL; TG: 132.33 ± 75.11 mg/dL). These may be possible due to reduced inflammation and improvements in oxidative stress, as demonstrated by the reduction of tumor necrosis factor- (TNF-) α and malondialdehyde (MDA), and the enhancement of glutathione (GSH) after consuming the RP jelly drink for 8 weeks. However, no significant differences of treatment on glucose, total cholesterol, MCP-1, interleukin-6, and interleukin-10 were observed. In conclusion, daily consumption of RP jelly drink for 8 weeks resulted in significant improvement in lipid profiles in subjects with dyslipidemia. However, more research is needed to assess its nutritional and functional potential.


Assuntos
Dislipidemias , Hibiscus , Adulto , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Biomarcadores , Quimiocina CCL2 , HDL-Colesterol , LDL-Colesterol , Método Duplo-Cego , Dislipidemias/tratamento farmacológico , Sucos de Frutas e Vegetais , Glucose , Glutationa/uso terapêutico , Humanos , Inflamação/tratamento farmacológico , Interleucina-10 , Interleucina-6 , Malondialdeído , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Polifenóis/farmacologia , Polifenóis/uso terapêutico , Triglicerídeos , Fatores de Necrose Tumoral/uso terapêutico
5.
Sci Rep ; 12(1): 18165, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36307527

RESUMO

In the present study, an eco-friendly process was made for the rapid synthesis of silver nanoparticles using aqueous leaf extract of Hibiscus sabdariffa. The process was characterized by Fourier Transform Infrared (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible and X-ray diffraction (XRD). These green silver nanoparticles (NPs) were used for mitigating the adverse effects of salinity on seed germination and growth parameters in plants. Accordingly, two experiments were conducted. In the first experiment, seven concentrations of green silver NPs and nine levels of NaCl:CaCl were apptoed on seeds for germination, and their effects were evaluated. In the second experiment, three concentrations of green silver NPs and NaCl were hypothesized to affect plant growth parameters. Seed germination, plant height, leaf, and root fresh and dry weights, as well as relative water content (RWC), decreased significantly under salt stress. However, green silver NPs intervened by alleviating the adverse effects of stress. Accordingly, green silver NPs were beneficial due to (1) activation of the antioxidant system by enhancing antioxidant enzymes such as catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD), and superoxide dismutase (SOD); (2) increase in the amounts of proline, soluble sugars and carbohydrates for osmoprotection; (3) improvements in flavonoid and anthocyanin contents. Real-time PCR showed that flavonoid and anthocyanin contents increased because of higher expressions in chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and anthocyanidin synthase (ANS) genes. In conclusion, green silver NPs offered an eco-friendly application for further research on agricultural development.


Assuntos
Hibiscus , Nanopartículas Metálicas , Prata/farmacologia , Hibiscus/metabolismo , Salinidade , Antioxidantes/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Antocianinas , Cloreto de Sódio , Extratos Vegetais/farmacologia , Difração de Raios X , Química Verde
6.
Biomed Res Int ; 2022: 1532987, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36312857

RESUMO

Kenaf (Hibiscus cannabinus L.) is a natural fibre crop that can be used for a variety of purposes and has various applications in industry. Despite this, its potential has not been fully exploited because of low yields and a narrow genetic base, limiting hybrids' development. Based on this background, eight kenaf mutants and one commercial cultivar were selected and crossed in a half-diallel for general and specific combining abilities (GCA and SCA) to get the desired results done in this investigation. The 36 hybrid offspring and their parental lines were tested in the field over two environments. Diallel results based on Griffing B method 2 indicated significant differences for all characters studied except for GCA in top diameter and plant height and top diameter SCA, indicating the existence of both additive and nonadditive gene actions for the inheritance of the traits. The amplitude of GCA variation was much higher than that of SCA variation for all parameters except top diameter and node number, showing the additive gene's prevalence and the likelihood of genetic advancement through selection. In both conditions, Hayman and Jinks graphical studies demonstrated that partial dominance controlled various fibre yield component parameters such as plant height, middle diameter, stick weight, and fibre weight. On the other hand, fibre yield and the majority of physical features indicated either dominance or overdominance gene action. Plant height, base diameter, core diameter, middle diameter, fresh stem weight, and stick weight all strongly positively correlated with fibre yield. These traits also had a higher proportion of additive effects, a moderate narrow-sense heritability, and a higher baker ratio, indicating successful indirect selection for fibre yield. The parents P1, P3, and P4 had the most dominant alleles for most of the features, while the parents P2, P7, and P9 had the most recessive alleles. The hybrids P1 × P4, P1 × P9, P2 × P3, P2 × P5, P4 × P6, P4 × P7, P4 × P9, P5 × P8, and P7 × P9 outperformed the parents in terms of heterotic responses and showed that they have a lot of genetic potential for kenaf enhancement in tropical climates.


Assuntos
Hibiscus , Hibiscus/genética , Clima Tropical , Vigor Híbrido , Estruturas Vegetais , Fibras na Dieta
7.
Nutrients ; 14(19)2022 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-36235722

RESUMO

Undernutrition and mineral deficiencies negatively impact both the health and academic performance of school children, while diets high in phytic acid and some phenolics inhibit the absorption of minerals such as iron and zinc. This study developed instant porridge powders rich in iron and zinc using pregelatinized chickpea flour (PCPF) and pregelatinized foxtail millet flour (PFMF) and assessed the potential of utilizing roselle calyx powder (RCP) as a source of organic acids to enhance its iron and zinc bioaccessibility. Physical properties, nutrients, mineral inhibitors and in vitro iron and zinc bioaccessibility of different proportions of PCPF, PFMF and RCP in instant porridge powders were evaluated. Three instant porridge powder formulations including instant chickpea powder (ICP) using PCPF, instant composite flour (ICF) using PCPF and PFMF and instant pulse porridge powder (IPP) using PCPF, PFMF and RCP were developed. Results show that all instant porridge powders were accepted by sensory evaluation, while different ingredients impacted color, consistency and the viscosity index. Addition of RCP improved the bioaccessibility of iron (1.3-1.6-fold) and zinc (1.3-1.9-fold). A 70 g serving of these instant porridge powders substantially contributed to daily protein, iron and zinc requirement for children aged 7-9 years. These porridge powders hold potential to serve as school meals for young children in low-to-middle income countries.


Assuntos
Hibiscus , Desnutrição , Criança , Pré-Escolar , Alimentos Fortificados , Humanos , Ferro , Minerais , Ácido Fítico , Pós , Zinco
8.
Molecules ; 27(18)2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36144809

RESUMO

Hibiscus sabdariffa L. (HS) has a long history of edible and medicinal uses. In this study, the biological activities of the extracts, chromatographic fractions, and hibiscus acid obtained from HS were evaluated for their potential bioactivities. Their ability to promote extracellular matrix synthesis in skin fibroblasts was evaluated by enzyme-linked immunosorbent assays. Their anti-inflammatory activity was evaluated in a nitric oxide (NO)-Griess inflammatory experiment. Furthermore, hibiscus acid was found to have a strong anti-oxidative stress effect through the establishment of an oxidative stress model induced by hydrogen peroxide. Several assays indicated that hibiscus acid treatment can effectively reduce extracellular adenosine triphosphate (ATP) secretion and carbonyl protein production, as well as maintain a high level of reduced/oxidized glutathione (GSH/GSSG) in skin cells, thus providing a possible mechanism by which hibiscus acid can counter antioxidative stress. The present study is the first to explore the reversing skin aging potential and the contributory component of HS.


Assuntos
Hibiscus , Envelhecimento da Pele , Trifosfato de Adenosina , Anti-Inflamatórios , Citratos , Dissulfeto de Glutationa , Hibiscus/química , Peróxido de Hidrogênio , Óxido Nítrico , Extratos Vegetais/química , Extratos Vegetais/farmacologia
9.
Int J Biol Macromol ; 220: 1512-1522, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36126810

RESUMO

Kenaf (Hibiscus cannabinus L.) seed is a valuable protein source that could be used to prepare protein hydrolysates with antihypertensive properties. However, the potential of using kenaf seeds for health food and pharmaceutical applications has not been fully exploited. Thus, the aim of this study was to identify and characterise the Angiotensin-I-Converting Enzyme (ACE) inhibitory peptides derived from the optimized hydrolysis conditions of kenaf seed protein hydrolysates (KSPH). The optimum hydrolysis conditions determined by response surface methodology (RSM) were as follows: temperature 65 °C, pH 6.5, hydrolysis time 2.25 h, and enzyme/substrate (E/S) ratio of 0.03 (w/w). Under these conditions, the degree of hydrolysis (DH) was 55.28 % and ACE inhibitory activity was 75.51 %. Also, the low molecular weight peptide fractions, <2 kilodalton (kDa) and 2-5 kDa showed the highest ACE-inhibitory activity (82.27 % and 83.69 %, respectively). The 2-5 kDa fraction by Quadrupole-Time-of-Flight Liquid Chromatography-Mass Spectrometry (QTOF LC - MS) revealed the abundance of six peptides, LYWSYLYN, ALFYWVS, LLLHAL, AKSCVVFP, INPPSTTN, and WTIPTPS. Kinetic studies showed that peptide LYWSYLYN possessed the highest Michaelis constant (Km), maximum velocity (Vmax) values and the lowest inhibitor constant (Ki) values, suggesting of its superior ACE inhibitory activity compared to other peptides.


Assuntos
Hibiscus , Hidrolisados de Proteína , Inibidores da Enzima Conversora de Angiotensina/química , Angiotensinas , Anti-Hipertensivos/química , Anti-Hipertensivos/farmacologia , Hidrólise , Cinética , Simulação de Acoplamento Molecular , Peptídeos/química , Peptidil Dipeptidase A/metabolismo , Preparações Farmacêuticas , Hidrolisados de Proteína/química , Sementes/metabolismo
10.
Nutrients ; 14(18)2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36145217

RESUMO

Phenanthrene (Phe) exposure is associated with skin ageing, cardiotoxicity and developmental defects. Here, we investigated the mode of Phe toxicity in human keratinocytes (HaCaT cells) and the attenuation of toxicity on pre-treatment (6 h) with ethanol extract of Hibiscus sabdariffa calyxes (HS). Cell viability, reactive oxygen species (ROS) generation, mitochondrial membrane potential (ΔΨm) alteration, changes in the transcriptional activity of selected genes involved in phase I and II metabolism, antioxidant response and gluconeogenesis, western blot and docking studies were performed to determine the protective effect of HS against Phe. Phe (250 µM) induced cytotoxicity in HaCaT cells through AhR-independent, CAR/PXR/RXR-mediated activation of CYP1A1 and the subsequent alterations in phase I and II metabolism genes. Further, CYP1A1 activation by Phe induced ROS generation, reduced ΔΨm and modulated antioxidant response, phase II metabolism and gluconeogenesis-related gene expression. However, pre-treatment with HS extract restored the pathological changes observed upon Phe exposure through CYP1A1 inhibition. Docking studies showed the site-specific activation of PXR and CAR by Phe and inhibition of CYP1A1 and CYP3A4 by the bioactive compounds of HS similar to that of the positive controls tested. Our results conclude that HS extract can attenuate Phe-induced toxicity in HaCaT cells through CAR/PXR/RXR mediated inhibition of CYP1A1.


Assuntos
Hibiscus , Fenantrenos , Extratos Vegetais/farmacologia , Receptores de Esteroides , Antioxidantes/farmacologia , Receptor Constitutivo de Androstano , Citocromo P-450 CYP1A1 , Citocromo P-450 CYP3A , Etanol , Células HaCaT , Humanos , Receptor de Pregnano X , Espécies Reativas de Oxigênio , Receptores Citoplasmáticos e Nucleares , Receptores de Esteroides/metabolismo
11.
Cells ; 11(18)2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36139349

RESUMO

Hibiscus sabdariffa L. (HSL) has high amounts of antioxidants and many beneficial effects in several pathologies. However, few studies describe the possible harmful effects of high concentrations of HSL. Here we evaluate the effect of excessive and chronic consumption of infusions with different percentages of HSL on some oxidative stress markers in serum, and the possible association with inflammation and increased systolic blood pressure (SBP), in healthy rats. A total of 32 male Wistar rats were used to form 4 groups with 8 animals each. Group 1 control (drinking tap water), group 2, 3 and 4, drinking water supplemented with 15, 30 and 60 g/L of HSL calyxes respectively. SBP was evaluated and determinations in serum of the NO3-/NO2- ratio, glutathione (GSH), total antioxidant capacity (TAC), selenium (Se), TNF-α, IL-1α/IL-1F1, IL-1ß, IL-10, extracellular superoxide dismutase (EcSOD), thioredoxin reductase (TrxR) and glutathione peroxidase (GPx) activities, were evaluated. The SBP (p = 0.01), GPx activity, GSH, TAC, Se, TNF-α and EcSOD activities (p ≤ 0.001) and IL-1α/IL-1F1, IL-1ß, TrxR and NO3-/NO2- (p ≤ 0.05), were increased but IL-10 (p < 0.001) was decreased in rats that consumed the 3 and 6% HSL infusions. The excessive and chronic consumption of HSL may increase the TAC that could lead to a proinflammatory state which is associated with hypertension.


Assuntos
Hibiscus , Extratos Vegetais , Animais , Antioxidantes/farmacologia , Pressão Sanguínea , Glutationa , Glutationa Peroxidase , Hibiscus/química , Inflamação , Interleucina-10 , Masculino , Dióxido de Nitrogênio , Extratos Vegetais/efeitos adversos , Ratos , Ratos Wistar , Selênio , Superóxido Dismutase , Tiorredoxina Dissulfeto Redutase , Fator de Necrose Tumoral alfa
12.
Niger J Physiol Sci ; 37(1): 147-152, 2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35947852

RESUMO

The aqueous calyx extract of Hibiscus sabdariffa (HS) is widely consumed as a beverage in Northern Nigeria and other parts of the world. HS has been reported to lower blood pressure (BP) in animals and man. However, not much is known about the effect of HS on BP in different postures. We tested the hypothesis that HS may lower BP, heart rate (HR) and heart rate-pressure product or double product (DP) by attenuating the discharge of the autonomic nervous system in different postures. Experiments were performed in accordance with the Principles of the Declaration of Helsinki. Following ethical approval and informed consent, BP and HR were measured in different postures (supine, sitting and standing) in apparently healthy human subjects (n=20) before and after (+HS) the oral administration of 15mg/Kg HS. Mean arterial pressure (MAP; taken as representative BP) and DP were calculated. Results are expressed as mean ±SEM. Paired t test and ANOVA with a post hoc Bonferroni test were used for statistical analyses. P<0.05 was considered significant. In the supine position MAP, HR and DP were significantly (P<0.0001 each) reduced in the presence of HS (85.6±1.7mmHg, 72.1±1.1/min and 8716±320mmHg.bpm) compared to its absence (89.6±2.0mmHg, 73.7±1.6/min and 8921±444mmHg.bpm). Similar trends were observed in the sitting position in the presence of HS (85.4±2.7mmHg, 73.7±1.8/min and 9098±345mmHg.bpm vs its absence: 91.4±2.3mmHg, 77.1±1.9/min and 9388±478mmHg.bpm; P<0.0001, P<0.0001 and P=0.007 respectively) and in the standing position (+HS: 89.3±2.0mmHg,         78.1±1.8/min and 10164±230mmHg.bpm vs its absence: 94.3±2.1mmHg, 81.8±2.3/min and 10742±268mmHg.bpm; P<0.0001, P<0.0001 and P=0.007 respectively). In the absence of HS, HR and DP were significantly higher in the standing posture (81.8±2.3/min, 10742±268mmHg.bpm) compared to the sitting (77.1±1.9/min, 9388±478mmHg.bpm; P<0.05 and P<0.0001 respectively) and the supine (73.7±1.6/min, 8921±444mmHg.bpm; P<0.001 each) postures while the BP remained similar. A similar trend was observed across          the three postures in the presence of HS although the parameters were significantly lower. It is concluded that HS lowered BP, HR and DP by modulating autonomic mechanisms through the inhibition of both parasympathetic withdrawal and sympathetic nervous system discharge across the postures. Also the standing posture is associated more with a higher sympathetic nervous system discharge and a higher cardiac oxygen demand and workload than the sitting and supine postures in the absence or presence of HS.


Assuntos
Hibiscus , Animais , Pressão Sanguínea/fisiologia , Frequência Cardíaca/fisiologia , Humanos , Masculino , Extratos Vegetais/farmacologia , Postura/fisiologia , Sujeitos da Pesquisa
13.
Niger J Physiol Sci ; 37(1): 113-117, 2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35947845

RESUMO

Hibiscus sabdariffa (HS) has gained attention as an anti-hypertensive agent. In the present study, we hypothesized that anthocyanins from HS may attenuate salt-induced hypertension in rats by suppressing the components of renin-angiotensin-aldoslestrone system (RAAS). Hypertension was induced in the rats by adding 8% NaCl in their diet for six weeks. Wistar rats (n=5 each) were randomly divided into seven groups. Group 1 was the normentensive control group and was fed with normal rat chew and water ad libitum; groups 2 and 3 served as hypertensive control (negative untreated and positive treated with captopril 30mg/kg respectively); groups 4, 5, and 6 served as treatment groups and were administered with graded doses of anthocyanins( 50, 100, 200mg/kg respectively) while group 7 received both 100mg anthocyanins and 30mg captopril per day for 4 weeks. Using HPLC, anthocyanins were isolated from HS calyx following standard protocol. Anthocyanins significantly (p<0.05) reduced blood pressure and heart rate in hypertensive rats in a dose dependent manner. The blood pressure reduction by anthocyanins was associated with a reduction in serum ACE and plasma aldosterone in the hypertensive rats. The effects of anthocyanins on blood pressure and on biomarkers of RAAS were similar to those of captopril, a reference anti-hypertensive drug. The results suggest that anthocyanins exerts a significant (p<0.05) anti-hypertensive potency on rats, probably mediated by the reduction in components of the RAAS. Keywords: hypertension, anthocyanins, renin, aldosterone, rats.


Assuntos
Antocianinas , Hibiscus , Hipertensão , Aldosterona , Animais , Antocianinas/farmacologia , Anti-Hipertensivos/farmacologia , Pressão Sanguínea , Captopril/uso terapêutico , Hipertensão/tratamento farmacológico , Ratos , Ratos Wistar , Sistema Renina-Angiotensina
14.
J Econ Entomol ; 115(5): 1592-1600, 2022 10 12.
Artigo em Inglês | MEDLINE | ID: mdl-35881549

RESUMO

Hibiscus mealybug (Nipaecoccus viridis, Newstead) is an emerging and serious pest of citrus in Florida. Preventing the spread of hibiscus mealybug by sanitizing personnel and equipment such as individual protective covers can help limit new infestations. Two mealybug life stages, first instars (crawlers) and eggs inside ovisacs, were tested as they are the most likely to be accidentally transported on people and tools. Isopropanol sprays in 3 commercially available concentrations (50%, 70%, and 90%) were tested against crawlers and ovisacs, and steam treatments and hot water immersion were tested against ovisacs. All concentrations of isopropanol resulted in >90% incapacitation of mealybug crawlers, especially when two sprays of isopropanol were applied. However, no concentration of isopropanol reliably killed all mealybug eggs within ovisacs. Steam treatments and hot water immersion for 10 min or longer at 49°C and for 5 min or longer at 54.5°C resulted in 100% mortality of mealybug adults and eggs inside ovisacs. However, adults and ovisacs insulated inside of individual protective covers did not experience 100% mortality until 30 min or longer at 49°C, 10 min or longer at 54.5°C, and 5 min or longer at 60°C for steam treatments. Isopropanol sprays can effectively incapacitate the majority of hibiscus mealybug crawlers on personnel and tools, but are not effective against ovisacs. Steam treatments and hot water immersion can reliably lead to 100% mortality of ovisacs, but longer times and higher temperatures must be used for steam treatments if ovisacs are insulated by equipment.


Assuntos
Citrus , Hemípteros , Hibiscus , 2-Propanol , Animais , Florida , Vapor , Água
15.
Biomed Pharmacother ; 153: 113383, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35820316

RESUMO

Development and identification of molecular compounds capable of killing or inhibiting transformed cells promoting carcinogenesis without inducing toxic effects to the normal cells are of utmost significance. A systematic review was conducted in screening for important literature was extensively performed by searching the Web of Science, Ovid, BMC Springer, Elsevier, Embase, and MEDLINE databases for optimum selectivity. Google Scholar was also used to supplement information. Pharmacotherapeutic biomolecules active against colon cancer carcinogenesis in Musa acuminata and Musa balbisiana (bananas), Punica granatum L (pomegranate), Glycine max (Soybean), Brassica oleracea L var. italica Plenck (Broccoli), and Hibiscus rosa-sinesis and Hibiscus sabdariffa (hibiscus) were evaluated. Signaling pathways like phosphatidylinositol 3-kinase (PI3K), mitogen-activated protein kinase (MAPK), protein kinase B (AKT), and nuclear factor-kappa B (NFκB) correlate the mediation of COX-2 expression. Increased levels of COX-2 are correlated with the occurrence and progression of colon cancer. Natural antioxidants in herbal plants including polyphenols and carotenoids inhibit the oxidation of lipids, proteins, and nucleic acids and thereby preventing the initiation of oxidizing chain reactions. These bioactive compounds should be considered an important dietary supplement.


Assuntos
Neoplasias do Colo , Hibiscus , Plantas Medicinais , Carcinogênese , Ciclo-Oxigenase 2 , Humanos , Fosfatidilinositol 3-Quinases
16.
Genes (Basel) ; 13(6)2022 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-35741783

RESUMO

Hibiscus syriacus, azalea, is an important woody ornamental shrub planted throughout many temperate and subtropical regions of the world. However, flower size is smaller in this species than some of its relatives. To increase flower size, interspecific hybridization has been used, and such hybrid cultivars are usually characterized by larger flowers, increased vigor, diverse leaf shapes, and reduced fertility. Our earlier studies have shown that these hybrid cultivars could backcross with H. syriacus when used as male parents. To understand the breeding potential of these hybrid cultivars, two popular tetraploid hybrid cultivars, 'Lohengrin' and 'Resi', were used as pollen parents to backcross several tetraploid H. syriacus cultivars. As a result, 28.76% and 64.4% of 'Lohengrin' and 'Resi' progenies exhibited larger flowers than both of their parents. Interestingly, 14 of 18 progenies of 'Resi' were putative hexaploids, whereas 19 tested 'Lohengrin' progenies were tetraploid. Because putative hexaploid progenies were only observed among progenies of 'Resi', this hybrid cultivar appears to produce unreduced gametes. In addition, among the 14 putative hexaploids derived from 'Resi', 11 had larger flowers than both of their parents and their tetraploid siblings (p < 0.05). The 45S rDNA and 5S rDNA locus segregation among those BC1F1 progenies was tested by fluorescent in situ hybridization (FISH), and the wide range of 45S rDNA signal numbers among siblings indicated that these aneuploids resulted from unequal segregation or chromosome rearrangement. Chromosome counting confirmed aneuploidy among BC1F1 progenies. Ploidy diversity and aneuploidy have been known to contribute to various elements of morphological diversity, such as larger flower size and reduced fertility, which are important in ornamental plant breeding. The present study demonstrated the breeding potential of interspecific Hibiscus cultivars for increasing ploidy level and flower size.


Assuntos
Hibiscus , Tetraploidia , Aneuploidia , Animais , DNA Ribossômico/genética , Hibiscus/genética , Hibridização in Situ Fluorescente/métodos , Melhoramento Vegetal
17.
J Econ Entomol ; 115(5): 1583-1591, 2022 10 12.
Artigo em Inglês | MEDLINE | ID: mdl-35686325

RESUMO

The Hibiscus mealybug, Nipaecoccus viridis (Newstead), has recently established in Florida citrus and become a pest of concern given secondary pest outbreaks associated with management of citrus greening disease. Chemical controls used to manage other citrus arthropod pests are not as effective against N. viridis due to its waxy secretions, clumping behavior, and induced cellular changes to host plant tissue which increase microhabitats. Populations of this mealybug pest are regulated by natural enemies in its native region, but it remains unclear if resident natural enemies in Florida citrus could similarly suppress N. viridis populations. This investigation: 1) established species-specific primers for N. viridis based on the mitochondrial gene Cytochrome-oxidase 1 (COI), 2) determined duration of N. viridis DNA detectability in a known predator, the mealybug destroyer (Cryptolaemus montrouzieri Mulsant), by using identified primers in molecular gut content analysis, and 3) screened field-collected predators for the presence of N. viridis DNA. The detection rate of N. viridis DNA was >50% at 36 h after adult C. montrouzieri feeding but DNA was no longer detectable by 72 h after feeding. Field-collected predators were largely comprised of spiders, lacewings, and C. montrouzieri. Spiders, beetles (primarily C. montrouzieri), and juvenile lacewings were the most abundant predators of N. viridis, with 17.8, 43.5, and 58.3 of field-collected samples testing positive for N. viridis DNA, respectively. Our results indicate that Florida citrus groves are hosts to abundant predators of N. viridis and encourage the incorporation of conservation or augmentative biological control for management of this pest.


Assuntos
Citrus , Besouros , Hemípteros , Hibiscus , Animais , Besouros/genética , Citocromos , DNA , Florida , Hemípteros/genética , Hibiscus/genética , Oxirredutases , Controle Biológico de Vetores/métodos
18.
Braz J Biol ; 84: e261123, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35674603

RESUMO

Nature is gifted with a wide range of ornamental plants, which beautify and clean the nature. Due to its great aesthetic value, there is a need to protect these plants from a variety of biotic and abiotic stresses. Hibiscus rosa-sinensis (L.) is an ornamental plant and it is commonly known as China rose or shoeblack plant. It is affected by several fungal and bacterial pathogens. Current study was designed to isolate leaf spot pathogen of H. rosa-sinensis and its control using silver nanoparticles (AgNPs). Based on molecular and morphological features, the isolated leaf spot pathogen was identified as Aspergillus niger. AgNPs were synthesized in the leaf extract of Calotropis procera and characterized. UV-vis spectral analysis displayed discrete plasmon resonance bands on the surface of synthesized AgNPs, depicting the presence of aromatic amino acids. Fourier transform infrared spectroscopy (FTIR) described the presence of C-O, NH, C-H, and O-H functional groups, which act as stabilizing and reducing molecules. X-ray diffraction (XRD) revealed the average size (~32.43 nm) of AgNPs and scanning electron microscopy (SEM) depicted their spherical nature. In this study, in vitro and in vivo antifungal activity of AgNPs was investigated. In vitro antifungal activity analysis revealed the highest growth inhibition of mycelia (87%) at 1.0 mg/ml concentration of AgNPs. The same concentration of AgNPs tremendously inhibited the spread of disease on infected leaves of H. rosa-sinensis. These results demonstrated significant disease control ability of AgNPs and suggested their use on different ornamental plants.


Assuntos
Calotropis , Hibiscus , Nanopartículas Metálicas , Rosa , Antifúngicos , Calotropis/metabolismo , Hibiscus/metabolismo , Nanopartículas Metálicas/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Folhas de Planta/metabolismo , Rosa/metabolismo , Prata/química , Prata/metabolismo , Prata/farmacologia
19.
Sci Rep ; 12(1): 9646, 2022 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-35688853

RESUMO

Nine morphologically distinct kenaf genotypes were hybridized to produce 36 hybrids following a half diallel mating design. The combining ability and gene action of 15 yield and yield components were assessed in hybrids and their parents across two environments. Except for the mid diameter and plant height traits, there were highly significant differences (p ≤ 0.01) between the environments and the interaction of genotype and environment. Additive gene effects were considerable for the inheritance of these traits, and the expression of these additive genes was heavily influenced by the environment. Significant differences were found for all studied traits for GCA except top diameter and SCA except plant height and top diameter, implying the presence of both additive and non-additive gene action for the inheritance of the concerned characters. For all features except top diameter and number of nodes, the magnitude of GCA variation was significantly higher than that of SCA variance, indicating the additive gene's predominance. The parental lines P1, P3 and P4 were outstanding general combiners for fiber yield and yield-related parameters. Considering combining ability and genetic analysis study, the crosses P1 × P4, P1 × P9, P2 × P3, P2 × P5, P4 × P6, P4 × P7, P4 × P9, P5 × P8, and P7 × P9 were found promising for their heterotic response to higher fiber yield, stick yield, seed yield and could be for future improvement in kenaf breeding programmes.


Assuntos
Hibiscus , Cruzamentos Genéticos , Hibiscus/genética , Vigor Híbrido , Melhoramento Vegetal , Clima Tropical
20.
Int J Mol Sci ; 23(12)2022 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-35743303

RESUMO

Cytoplasmic male sterility (CMS) is widely exploited in hybrid seed production. Kenaf is an important fiber crop with high heterosis. The molecular mechanism of kenaf CMS remains unclear, particularly in terms of DNA methylation. Here, using the anthers of a kenaf CMS line (P3A) and its maintainer line (P3B), comparative physiological, DNA methylation, and transcriptome analyses were performed. The results showed that P3A had considerably lower levels of IAA, ABA, photosynthetic products and ATP contents than P3B. DNA methylome analysis revealed 650 differentially methylated genes (DMGs) with 313 up- and 337 down methylated, and transcriptome analysis revealed 1788 differentially expressed genes (DEGs) with 558 up- and 1230 downregulated genes in P3A compared with P3B. Moreover, 45 genes were characterized as both DEGs and DMGs, including AUX,CYP, BGL3B, SUS6, AGL30 and MYB21. Many DEGs may be regulated by related DMGs based on methylome and transcriptome studies. These DEGs were involved in carbon metabolism, plant hormone signal transduction, the TCA cycle and the MAPK signaling pathway and were shown to be important for CMS in kenaf. These results provide new insights into the epigenetic mechanism of CMS in kenaf and other crops.


Assuntos
Hibiscus , Infertilidade das Plantas , Metilação de DNA , Epigenoma , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Hibiscus/genética , Hibiscus/metabolismo , Infertilidade das Plantas/genética , Transcriptoma
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