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1.
Braz. j. oral sci ; 21: e225924, jan.-dez. 2022. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1355003

RESUMO

Aim: To assess the reliability and validity of morphometric features on 3D digital models produced by scanning maxillary dental casts of Malaysian Malay subjects. Methods: Dental casts of 20 subjects were scanned using a 3D laser scanner (Next Engine Inc., Santa Monica, California, USA). The palatal rugae morphometric features were assessed on the resulting 3D models using 3-Matic Research 9.0 software (Materialise NV, Heverlee, Belgium). The assessments were repeated by the first and second authors to assess the intra- and interexaminer reliability, respectively. Rugae morphometric features were also evaluated on the conventional plaster models to assess the validity of the 3D method. Results: Kappa values of the validity ranged from 0.807 to 0.922 for rugae shape, size category and direction. The intraclass correlation coefficient (ICC) for rugae number validity was 0.979. For intra-examiner reliability, kappa values ranged from 0.716-1.000 for rugae shape, size category and direction. The ICC for rugae number intra-examiner reliability was 0.949. Kappa values of interexaminer reliability for rugae shape, size category and direction were 0.723-885, while the ICC of rugae number was 0.896. Conclusion: Palatal rugae analyses on 3D digital models scanned by the 3D Next Engine laser scanner using 3-Matic Research 9.0 software are valid and reliable


Assuntos
Palato , Antropologia Forense , Imageamento Tridimensional , Odontologia Legal
2.
Braz. j. oral sci ; 21: e227903, jan.-dez. 2022. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1355005

RESUMO

Aim: To evaluate the accuracy and the validity of orthodontic diagnostic measurements, as well as virtual tooth transformations using a generic open access 3D software compared to OrthoAnalyzer (3Shape) software; which was previously tested and proven for accuracy. Methods: 40 maxillary and mandibular single arch study models were duplicated and scanned using 3Shape laser scanner. The files were imported into the generic and OrthoAnalyzer software programs; where linear measurements were taken twice to investigate the accuracy of the program. To test the accuracy of the program format, they were printed, rescanned and imported into OrthAnalyzer. Finally, to investigate the accuracy of editing capabilities, linear and angular transformation procedures were performed, superimposed and printed to be rescanned and imported to OrthoAnalyzer for comparison. Results: There was no statistically significant difference between the two groups using the two software programs regarding the accuracy of the linear measurements (p>0.05). There was no statistically significant difference between the different formats among all the measurements, (p>0.05). The editing capabilities also showed no statistically significant difference (p>0.05). Conclusion: The generic 3D software (Meshmixer) was valid and accurate in cast measurements and linear and angular editing procedures. It can be used for orthodontic diagnosis and treatment planning without added costs


Assuntos
Software , Moldes Cirúrgicos , Imageamento Tridimensional , Modelos Dentários
3.
Comput Intell Neurosci ; 2022: 1023865, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35528367

RESUMO

The purpose of this paper is to understand the digital 3D multimedia panoramic visual communication technology based on virtual reality. Firstly, the key concepts and characteristics of virtual reality are introduced, including the development and application of digital three-dimensional panorama technology. Then, according to the theoretical research, some basic knowledge of 3D panoramic image Mosaic is introduced, including camera image modeling, image sharing, and image exchange. Finally, with the development of the virtual tour at the College of Normal University, the hardware of panoramic technology and the demand of panoramic image search have been expanded in the application. The design of panoramic Mosaic, panoramic image generation, and virtual tour school construction considers real-world issues. The innovation of this paper lies in that will be used by SketchUp8.0 software builds the geometry of 3d virtual scene and by the cylindrical panoramic images based on image of building 3 d virtual scene organic unifies in together and makes a panoramic image can be as the change of seasons in the real scene and real-time change, enhance the sense of the reality of the system and user immersive.


Assuntos
Realidade Virtual , Humanos , Imageamento Tridimensional , Software , Tecnologia
4.
Planta ; 255(6): 118, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35522384

RESUMO

MAIN CONCLUSION: Focused ion beam scanning electron microscopy is well suited for volumetric extractions and 3D reconstructions of plant cells and its organelles. The three-dimensional (3D) reconstruction of individual plant cells is an important tool to extract volumetric data of organelles and is necessary to fully understand ultrastructural changes and adaptations of plants to their environment. Methods such as the 3D reconstruction of cells based on light microscopical images often lack the resolution necessary to clearly reconstruct all cell compartments within a cell. The 3D reconstruction of cells through serial sectioning transmission electron microscopy (ssTEM) and focused ion beam scanning electron microscopy (FIB-SEM) are powerful alternatives but not widely used in plant sciences. Here, we present a method for the 3D reconstruction and volumetric extraction of plant cells based on FIB milling and compare the results with 3D reconstructions obtained with ssTEM. When compared to 3D reconstruction based on ssTEM, FIB-SEM delivered similar results. The data extracted in this study demonstrated that tobacco cells were larger (31410 µm3) than pumpkin cells (20697 µm3) and contained more chloroplasts (175 vs. 124), mitochondria (1317 vs. 291) and peroxisomes (745 vs. 79). While individual chloroplasts, mitochondria, peroxisomes were larger in pumpkin plants (25, 53, and 50%, respectively) they covered more total volume in tobacco plants (5390, 395, 374 µm3, respectively) due to their higher number per cell when compared to pumpkin plants (4762, 134, 59 µm3, respectively). While image acquisition with FIB-SEM was automated, software controlled, and less difficult than ssTEM, FIB milling was slower and sections could not be revised or re-imaged as they were destroyed by the ion beam. Nevertheless, the results in this study demonstrated that both, FIB-SEM and ssTEM, are powerful tools for the 3D reconstruction of and volumetric extraction from plant cells and that there were large differences in size, number, and organelle composition between pumpkin and tobacco cells.


Assuntos
Imageamento Tridimensional , Células Vegetais , Imageamento Tridimensional/métodos , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Folhas de Planta
5.
BMC Pregnancy Childbirth ; 22(1): 395, 2022 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-35525936

RESUMO

BACKGROUND: To investigate the safety and effectiveness of tubal inflammatory drugs in patients with incomplete tubal obstruction of at least one side after four-dimensional hysterosalpingo-contrast-sonography (4D-HyCoSy) examination. METHODS: Two hundred fifteen cases of tubal incomplete obstruction were diagnosed by ultrasonography from February 2019 to November 2020.According to retrospective analysis,the patients in this study were divided into experimental and control groups; the experimental group combined with salpingitis drugs, and the control group received blank control. Basic information, degree of pain, postoperative complications, and pregnancy rate were then compared between the two groups. RESULTS: Compared with the control group, there was no significant difference in the basic information; in preoperative, intraoperative, or postoperative pain; or in postoperative complications (P > 0.05). The cumulative pregnancy rate of the experimental group (26.8%) was statistically different from that of the control group (14.4%) (P < 0.05). CONCLUSIONS: We observed that for infertile patients with incomplete obstruction of at least one fallopian tube as diagnosed by contrast-enhanced ultrasonography, salpingitis-treatment drugs effectively improved the pregnancy rate postoperatively, with high effectiveness and safety. This regimen is thus worthy of further investigation and promotion in the future.


Assuntos
Doenças das Tubas Uterinas , Infertilidade Feminina , Salpingite , Meios de Contraste/efeitos adversos , Doenças das Tubas Uterinas/complicações , Doenças das Tubas Uterinas/diagnóstico por imagem , Testes de Obstrução das Tubas Uterinas/efeitos adversos , Testes de Obstrução das Tubas Uterinas/métodos , Tubas Uterinas/diagnóstico por imagem , Feminino , Humanos , Histerossalpingografia/efeitos adversos , Histerossalpingografia/métodos , Imageamento Tridimensional/métodos , Infertilidade Feminina/diagnóstico , Infertilidade Feminina/etiologia , Complicações Pós-Operatórias/etiologia , Gravidez , Estudos Retrospectivos , Salpingite/complicações , Salpingite/diagnóstico por imagem , Ultrassonografia/métodos
6.
Methods Mol Biol ; 2469: 201-218, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35508841

RESUMO

Digital imaging using high-resolution X-ray micro-computed tomography has become a methodology with increasing importance for assessing wood tissues. This chapter aims to describe the use of this nondestructive, noninvasive, and reproducible technique to new researchers interested in analyzing the three-dimensional properties of the resin duct systems in pine stem wood samples.


Assuntos
Imageamento Tridimensional , Imageamento Tridimensional/métodos , Microtomografia por Raio-X
7.
Adv Exp Med Biol ; 1364: 349-372, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35508883

RESUMO

In recent years, the amount of publications related to the study of spine using ultrasound, especially on scoliosis, has rapidly increased. Many new methods and application related to 3D ultrasound imaging for scoliosis assessment have been recently reported. The capabilities of three-dimensional (3D) ultrasound to visualize the vertebrae features such as spinous and transverse processes, bilateral laminae and ribs mainly at the level of main thoracic and thoracolumbar region are shown in this chapter. Different ultrasound systems developed for scoliosis evaluation and application of ultrasound during different postures and treatments such as brace fitting are also introduced. Moreover, innovative measurement methods for spinal evaluation in orthogonal planes are presented in this chapter. The advance of the development of 3D ultrasound allows one to perform large-scale screening, prognosticate and diagnose mild and severe scoliosis at a more frequent and non-ionizing basis. The authors wish that this chapter will help to promote the applications of ultrasound to medical specialists in different aspects regarding the possibilities of ultrasonography to tackle with different spinal problems.


Assuntos
Escoliose , Humanos , Imageamento Tridimensional/métodos , Postura , Escoliose/diagnóstico por imagem , Coluna Vertebral/diagnóstico por imagem , Ultrassonografia
8.
PLoS One ; 17(5): e0267742, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35511774

RESUMO

The advent of intraoral scanning methods has caused a paradigm shift in dentistry. However, despite their many advantages, intraoral scanners cannot accurately recognize the metallic surfaces of prothesis. Therefore, this experiment was designed to verify the effect of scanning-aid agents on the scanning accuracy using metallic reference models. Three different types of metallic reference models (inlay, onlay, and bridge) were specially designed and produced using a milling machine to simulate intraoral dental restorations. Three experimental groups (application of ScanCure, IP Scan Spray, and VITA Powder Scan Spray) were set up and scanned images (each n = 5) were acquired using the I500® intraoral scanner. The reference datasets were established by a 3D design that reflected the deviations between the measured distances and previously planned distances on the reference models. All acquired experimental datasets were digitally superimposed and compared with the reference datasets. Intragroup comparisons (precision, n = 10) were also performed. The root mean square (RMS) values of trueness in the ScanCure and IP groups were significantly more accurate than those of the VITA group in the inlay and onlay reference models (p < 0.05). Notably, in the bridge reference model, the liquid-type ScanCure group showed the highest accuracy of trueness, with statistical significance (p < 0.05). However, the RMS values of precision were not significantly different among the groups. These findings suggest that liquid-type scanning agents can be effectively used to obtain more accurate scan images of intraoral metallic dental restorations.


Assuntos
Desenho Assistido por Computador , Técnica de Moldagem Odontológica , Imageamento Tridimensional , Cintilografia
9.
J Forensic Odontostomatol ; 1(40): 53-64, 2022 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-35499537

RESUMO

Sex determination is one of the primary concerns of forensic science. The cranial bones, pelvis, and mandible have been used for determining the sex of specimens. Because the mandible is robust and sexually dimorphic, studies have evaluated its metric and morphological traits. This study was designed as a retrospective study involving cone beam computed tomography (CBCT) images to assess sexual dimorphism of the mandible in the Turkish population. Total sample group consisted of 176 bimaxillary CBCT scans (71 males and 102 females; ages 19-67 years). Sixteen mandibular parameters were measured using two different software programmes. Measurements were recorded in various planes of three-dimensional (3D) reconstructions of the scans. All parameters aside from SIMaCD were found to be statistically significant. The highest diagnostic accuracy rate was associated with IMaF, and the overall accuracy rate of the fourteen parameters was found to be 80%.


Assuntos
Determinação do Sexo pelo Esqueleto , Tomografia Computadorizada de Feixe Cônico Espiral , Adulto , Idoso , Feminino , Antropologia Forense , Humanos , Imageamento Tridimensional , Masculino , Mandíbula/diagnóstico por imagem , Pessoa de Meia-Idade , Estudos Retrospectivos , Caracteres Sexuais , Determinação do Sexo pelo Esqueleto/métodos , Adulto Jovem
10.
Facial Plast Surg Clin North Am ; 30(2): 149-158, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35501052

RESUMO

Facial measurements serve as a valuable tool in the treatment planning of facial plastic surgery. The aim of this study was to evaluate the accuracy and reliability of standard 3D anthropometric measurements of the face made with one low-cost handheld 3D scanner and one industrial-type mobile 3D scanner. There are clear potential benefits of using 3D measurements by means of new handheld mobile scanners. However, the Sense scanner from the class of inexpensive scanners showed significant limitations in more complex areas such as the lip and nose, whereas proportions could be measured satisfactorily.


Assuntos
Face , Imageamento Tridimensional , Face/diagnóstico por imagem , Humanos , Imageamento Tridimensional/métodos , Nariz , Reprodutibilidade dos Testes
11.
Facial Plast Surg Clin North Am ; 30(2): 159-166, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35501053

RESUMO

Three-dimensional surface imaging (3DSI) has been shown to be a useful tool for plastic surgeons in the preoperative, intraoperative, and postoperative setting. The objective of this investigation was to compare the accuracy of facial surface distance measurements using both a handheld facial 3DSI device and a stationary whole-body 3DSI device. Users should be aware of deviations when obtaining 3DSI using the presented imaging devices but should not refrain from using them, as the absolute differences might be too small to play a role in both, clinical and research, settings.


Assuntos
Face , Imageamento Tridimensional , Face/diagnóstico por imagem , Face/cirurgia , Humanos , Imageamento Tridimensional/métodos
12.
Sci Rep ; 12(1): 7229, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35508477

RESUMO

Millimeter-scale multi-cellular level imagers enable various applications, ranging from intraoperative surgical navigation to implantable sensors. However, the tradeoffs for miniaturization compromise resolution, making extracting 3D cell locations challenging-critical for tumor margin assessment and therapy monitoring. This work presents three machine-learning-based modules that extract spatial information from single image acquisitions using custom-made millimeter-scale imagers. The neural networks were trained on synthetically-generated (using Perlin noise) cell images. The first network is a convolutional neural network estimating the depth of a single layer of cells, the second is a deblurring module correcting for the point spread function (PSF). The final module extracts spatial information from a single image acquisition of a 3D specimen and reconstructs cross-sections, by providing a layered "map" of cell locations. The maximum depth error of the first module is 100 µm, with 87% test accuracy. The second module's PSF correction achieves a least-square-error of only 4%. The third module generates a binary "cell" or "no cell" per-pixel labeling with an accuracy ranging from 89% to 85%. This work demonstrates the synergy between ultra-small silicon-based imagers that enable in vivo imaging but face a trade-off in spatial resolution, and the processing power of neural networks to achieve enhancements beyond conventional linear optimization techniques.


Assuntos
Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Processamento de Imagem Assistida por Computador/métodos , Aprendizado de Máquina , Redes Neurais de Computação
13.
Commun Biol ; 5(1): 431, 2022 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-35534680

RESUMO

Various optical clearing approaches have been introduced to meet the growing demand for 3D volume imaging of biological structures. Each has its own strengths but still suffers from low transparency, long incubation time, processing complexity, tissue deformation, or fluorescence quenching, and a single solution that best satisfies all aspects has yet been developed. Here, we develop OptiMuS, an optimized single-step solution that overcomes the shortcomings of the existing aqueous-based clearing methods and that provides the best performance in terms of transparency, clearing rate, and size retention. OptiMuS achieves rapid and high transparency of brain tissues and other intact organs while preserving the size and fluorescent signal of the tissues. Moreover, OptiMuS is compatible with the use of lipophilic dyes, revealing DiI-labeled vascular structures of the whole brain, kidney, spleen, and intestine, and is also applied to 3D quantitative and comparative analysis of DiI-labeled vascular structures of glomeruli turfs in normal and diseased kidneys. Together, OptiMuS provides a single-step solution for simple, fast, and versatile optical clearing method to obtain high tissue transparency with minimum structural changes and is widely applicable for 3D imaging of various whole biological structures.


Assuntos
Imageamento Tridimensional , Imagem Óptica , Encéfalo/diagnóstico por imagem , Corantes , Imageamento Tridimensional/métodos , Imagem Óptica/métodos
14.
J Synchrotron Radiat ; 29(Pt 3): 843-852, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35511016

RESUMO

X-ray nano-tomography with phase contrast (nanoCT) using synchrotron radiation is a powerful tool to non-destructively investigate 3D material properties at the nanoscale. In large bone lesions, such as severe bone fractures, bone cancer or other diseases, bone grafts substituting the lost bone might be necessary. Such grafts can be of biological origin or be composed of a synthetic bone substitute. The long-term functioning of artificial bone substitutes depends on many factors. Synchrotron nanoCT imaging has great potential to contribute to further the understanding of integration of implants into bone tissue by imaging the spatial interaction between bone tissue and implant, and by accessing the interface between implant material and bone tissue. With this aim, a methodology for evaluating the image quality is presented for in-line phase contrast nanoCT images of bone scaffold samples. A PMMA-embedded tricalcium phosphate scaffold was used with both a closed and an open porosity structure and bone ingrowths as a representative system of three known materials. Parameters such as spatial resolution and signal-to-noise ratio were extracted and used to explore and quantitatively compare the effects of implementation choices in the imaging setup, such as camera technology and imaging energy, on the resulting image quality. Increasing the X-ray energy from 17.5 keV to 29.6 keV leads to a notable improvement in image quality regardless of the camera technology used, with the two tested camera setups performing at a comparable level when the recorded intensity was kept constant.


Assuntos
Materiais Biocompatíveis , Tomografia Computadorizada por Raios X , Osso e Ossos/diagnóstico por imagem , Imageamento Tridimensional/métodos , Microscopia de Contraste de Fase , Síncrotrons
15.
PLoS One ; 17(5): e0267943, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35511770

RESUMO

Proper placement of suture anchors is an important step in Bankart repair as improper placement can lead to failure. Concern surrounding suture anchor placement inspired the use navigation systems in shoulder arthroscopy. We aimed to demonstrate the technological advantage of using the O-arm (Medtronic Navigation, Denver, CO, USA) image guidance system to provide real-time images during portal and anchor placements in shoulder arthroscopy. Consecutive patients (from July to October 2014) who were admitted for arthroscopic capsulolabral repair surgeries were included. Ten patients were randomly enrolled in the navigation group and 10 in the traditional group. The glenoid was divided into four zones, and the penetration rates in each zone were compared between the two groups. In zone III, the most inferior region of the glenoid, the penetration rate was 40.9% in the traditional group and 15.7% in the navigation group (P = 0.077), demonstrating a trend toward improved accuracy of anchor placement with the aid of the navigation system; however, this was not statistically significant. Average surgical time in the navigation and traditional groups was 177.6±40.2 and 117.7±17.6 mins, respectively. American Shoulder and Elbow Surgeons Shoulder Scores showed no difference before and 6 months after surgery. This pilot study showed a trend toward decreased penetration rate in O-arm-navigated capsulolabral repair surgeries and decreased risks of implant misplacement; however, possibly due to the small sample size, the difference was not statistically significant. Further large-scale studies are needed to confirm the possible benefit of the navigation system. Even with the use of navigation systems, there were still some penetrations in zone III of the glenoid. This penetration may be attributed to the micro-motion of the acromioclavicular joint. Although the navigation group showed a significant increase in surgical time, with improvements in instrument design, O-arm-navigated arthroscopy will gain popularity in clinical practice.


Assuntos
Instabilidade Articular , Articulação do Ombro , Cirurgia Assistida por Computador , Artroscopia/métodos , Humanos , Imageamento Tridimensional , Instabilidade Articular/cirurgia , Projetos Piloto , Ombro/cirurgia , Articulação do Ombro/cirurgia , Âncoras de Sutura , Tomografia Computadorizada por Raios X
16.
Acta Crystallogr D Struct Biol ; 78(Pt 4): 410-423, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-35362465

RESUMO

Cryo-electron microscopy (cryoEM) has become a well established technique to elucidate the 3D structures of biological macromolecules. Projection images from thousands of macromolecules that are assumed to be structurally identical are combined into a single 3D map representing the Coulomb potential of the macromolecule under study. This article discusses possible caveats along the image-processing path and how to avoid them to obtain a reliable 3D structure. Some of these problems are very well known in the community. These may be referred to as sample-related (such as specimen denaturation at interfaces or non-uniform projection geometry leading to underrepresented projection directions). The rest are related to the algorithms used. While some have been discussed in depth in the literature, such as the use of an incorrect initial volume, others have received much less attention. However, they are fundamental in any data-analysis approach. Chiefly among them, instabilities in estimating many of the key parameters that are required for a correct 3D reconstruction that occur all along the processing workflow are referred to, which may significantly affect the reliability of the whole process. In the field, the term overfitting has been coined to refer to some particular kinds of artifacts. It is argued that overfitting is a statistical bias in key parameter-estimation steps in the 3D reconstruction process, including intrinsic algorithmic bias. It is also shown that common tools (Fourier shell correlation) and strategies (gold standard) that are normally used to detect or prevent overfitting do not fully protect against it. Alternatively, it is proposed that detecting the bias that leads to overfitting is much easier when addressed at the level of parameter estimation, rather than detecting it once the particle images have been combined into a 3D map. Comparing the results from multiple algorithms (or at least, independent executions of the same algorithm) can detect parameter bias. These multiple executions could then be averaged to give a lower variance estimate of the underlying parameters.


Assuntos
Imageamento Tridimensional , Viés , Consenso , Microscopia Crioeletrônica/métodos , Imageamento Tridimensional/métodos , Reprodutibilidade dos Testes
17.
Sci Rep ; 12(1): 6711, 2022 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-35468910

RESUMO

Three-dimensional integrated circuit (3D IC) technologies have been receiving much attention recently due to the near-ending of Moore's law of minimization in 2D IC. However, the reliability of 3D IC, which is greatly influenced by voids and failure in interconnects during the fabrication processes, typically requires slow testing and relies on human's judgement. Thus, the growing demand for 3D IC has generated considerable attention on the importance of reliability analysis and failure prediction. This research conducts 3D X-ray tomographic images combining with AI deep learning based on a convolutional neural network (CNN) for non-destructive analysis of solder interconnects. By training the AI machine using a reliable database of collected images, the AI can quickly detect and predict the interconnect operational faults of solder joints with an accuracy of up to 89.9% based on non-destructive 3D X-ray tomographic images. The important features which determine the "Good" or "Failure" condition for a reflowed microbump, such as area loss percentage at the middle cross-section, are also revealed.


Assuntos
Inteligência Artificial , Aprendizado Profundo , Humanos , Imageamento Tridimensional/métodos , Redes Neurais de Computação , Reprodutibilidade dos Testes
18.
Commun Biol ; 5(1): 388, 2022 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-35468989

RESUMO

Recent advances in brain clearing and imaging have made it possible to image entire mammalian brains at sub-micron resolution. These images offer the potential to assemble brain-wide atlases of neuron morphology, but manual neuron reconstruction remains a bottleneck. Several automatic reconstruction algorithms exist, but most focus on single neuron images. In this paper, we present a probabilistic reconstruction method, ViterBrain, which combines a hidden Markov state process that encodes neuron geometry with a random field appearance model of neuron fluorescence. ViterBrain utilizes dynamic programming to compute the global maximizer of what we call the most probable neuron path. We applied our algorithm to imperfect image segmentations, and showed that it can follow axons in the presence of noise or nearby neurons. We also provide an interactive framework where users can trace neurons by fixing start and endpoints. ViterBrain is available in our open-source Python package brainlit.


Assuntos
Imageamento Tridimensional , Neurônios , Algoritmos , Animais , Encéfalo , Imageamento Tridimensional/métodos , Mamíferos , Cadeias de Markov
19.
Proc Natl Acad Sci U S A ; 119(18): e2118483119, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35476531

RESUMO

SignificanceMany human diseases are causally linked to the gut microbiota, yet the field still lacks mechanistic understanding of the underlying complex interactions, because existing tools cannot simultaneously quantify microbial communities and their native context. In this work, we provide an approach to tissue clearing and preservation that enables 3D visualization of the biogeography of the host-microbiota interface. We combine this tool with sequencing and multiplexed microbial labeling to provide the field with a platform on which to discover patterns in the spatial distribution of microbes. We validated this platform by quantifying bacterial distribution in cecal mucosa at different stages of antibiotic exposure. This approach may enable researchers to formulate and test new hypotheses about host-microbe and microbe-microbe interactions.


Assuntos
Microbioma Gastrointestinal , Microbiota , Bactérias , Humanos , Imageamento Tridimensional , Mucosa Intestinal/microbiologia
20.
Sci Rep ; 12(1): 6835, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35478214

RESUMO

Magnetic resonance vessel wall imaging is desirable for evaluating Kawasaki disease (KD)-associated coronary arterial lesions. To evaluate the reproducibility of three-dimensional turbo spin-echo (3D-TSE) and two-dimensional dual inversion-recovery turbo spin-echo (2D-DIR-TSE) for coronary vessel wall imaging in KD. Ten patients were prospectively enrolled. Coronary vessel wall imaging with axial-slice orientation 3D-TSE and 2D-DIR-TSE were acquired for cross-sectional images in aneurysmal and normal regions. Lumen area (LA), wall area (WA), and normalized wall index (NWI) of cross-sectional images were measured in both regions. Reproducibility between 3D-TSE and 2D-DIR-TSE was evaluated via intraclass correlation coefficients (ICCs) and Bland-Altman plots. 48 points (aneurysmal, 27; normal, 21) were evaluated. There were high ICCs between 3D-TSE and 2D-DIR-TSE in LA (0.95) and WA (0.95). In aneurysmal regions, 95% limits of agreement were LA, WA, and NWI of - 29.9 to 30.4 mm2, - 18.8 to 15.0 mm2, and - 0.22 to 0.20, respectively. In normal regions, the 95% limits of agreement were LA, WA, and NWI of - 4.44 to 4.38 mm2, - 3.51 to 4.30 mm2, and - 0.14 to 0.16, respectively. No fixed and proportional biases between 3D-TSE and 2D-DIR-TSE images in aneurysmal and normal regions were noted. 3D-TSE was reproducible with conventional 2D-DIR-TSE for coronary vessel wall assessment on KD.


Assuntos
Vasos Coronários , Síndrome de Linfonodos Mucocutâneos , Vasos Coronários/diagnóstico por imagem , Humanos , Imageamento Tridimensional/métodos , Imageamento por Ressonância Magnética/métodos , Síndrome de Linfonodos Mucocutâneos/diagnóstico por imagem , Reprodutibilidade dos Testes
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