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1.
Food Chem ; 399: 133918, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35994858

RESUMO

A core-shell magnetic sulfonatocalix[6]arene covalently cross-linked polymer was proposed as a magnetic adsorbent, combined with ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) for the enrichment and determination of epoxy derivatives in canned foods. The adsorbent has high density of host-guest recognition functional groups, abundant binding sites and suitable cavity size, showing good extraction performance for epoxy derivatives. Quantum chemical simulation calculations provedmultiple interaction forces in the adsorption process. Theextractionparameterswere investigated. Under optimized experimental conditions, 13 kinds of target analytes showed low detection limits (0.0072-0.023 ng/g) and good precisions (RSDs of 0.8 %-9.4 %). This method has been successfully applied to the simultaneous determination of 13 kinds of epoxy derivatives in different food samples including canned beverage, fish, meat, and milk powder. Satisfactory recoveries (74.9 %-118 %) were obtained. The results showed the potential application prospects in the enrichment and detection of hazardous substances in food.


Assuntos
Éter , Espectrometria de Massas em Tandem , Animais , Cromatografia Líquida de Alta Pressão/métodos , Alimentos em Conserva/análise , Glicerol , Éteres de Glicerila , Indicadores e Reagentes , Fenômenos Magnéticos , Polímeros/análise , Extração em Fase Sólida/métodos , Espectrometria de Massas em Tandem/métodos
2.
Spectrochim Acta A Mol Biomol Spectrosc ; 287(Pt 2): 122125, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36410174

RESUMO

A simple, precise and inexpensive spectrofluorimetric method has been developed for assay of rivaroxaban raw material and its tablets. The method depends on the quenching effect of rivaroxaban on the fluorescence intensity of acetoxymercuric fluorescein (AMF). Parameters that may affect the reaction such as pH, AMF solution concentration, reaction time and diluting solvents were studied and optimized. The proposed method was applied for determination of rivaroxaban in tablets with percentage recovery of 100.4 ± 0.28, and in organic extract of spiked plasma samples with percentage recovery of 98.40 ± 1.08. The developed method was validated according to ICH guidelines in terms of accuracy, precision, linearity, range, limit of detection (LOD) and limit of quantification (LOQ).


Assuntos
Bioensaio , Rivaroxabana , Indicadores e Reagentes , Fluoresceína , Preparações Farmacêuticas
3.
Anal Chim Acta ; 1237: 340610, 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36442939

RESUMO

The relevance of the problem of urine tampering is well-known in forensic toxicology, with sample dilution being the most used method to cheat toxicological controls. Among the criteria to assess urine integrity, the quantification of creatinine probably represents the most popular method. The present paper presents a simple and low-cost analytical device for on-site creatinine determination as first-line screening for urine dilution. The proposed microfluidic devices were designed as a three-dimensional origami pattern. The device included three colorimetric reactions based on picric acid (PA-based reagent), 3,5-dinitrobenzoic acid (DNBA-based reagent), and Nessler's reagent. The last one, to the best of our knowledge, has never been used before for creatinine determination. In order to assure the highest ease and economy of operation, the color detection and data processing were performed using a built-in smartphone camera and the associated software. The optimized device showed a detection limit of 0.02 g/L. The proposed method was used for the qualitative screening for urine dilution of 48 samples, showing a diagnostic sensitivity and specificity for PA-based, DNBA-based and Nessler's reagent of 83.3%-80.0%, 72.2%-70.0%, and 100.0%-93.3% respectively, versus reference enzymatic method adopting a cut-off of 0.2 g/L. In conclusion, the present preliminary study shows that the proposed device could be a useful tool for on-site screening for urine tampering at the time of sample collection for toxicological testing.


Assuntos
Colorimetria , Dispositivos Lab-On-A-Chip , Creatinina , Indicadores e Reagentes , Microfluídica
4.
Chemosphere ; 311(Pt 1): 136887, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36257393

RESUMO

In the present paper, synthesis of SBA-15 nanoparticles was carried out from tetraethyl orthosilicate (TEOS) precursor using the sol-gel process. After being combined with Poly propylene imine, and ZIF-8 they were employed for the removal of Penicillin G. The synthesized combination morphology was assessed using nitrogen adsorption and desorption (BET), Fourier transform infrared (FTIR) spectroscopy, and X-ray powder diffraction (XRD). The parameters affecting Penicillin G removal, including pH, amount of adsorbent, time of contact, temperature, and concentration, were optimized, and the optimum levels of the mentioned variables were reported to be pH = 3, 0.03 g, 90 min, 25 °C, and 100 ppm, respectively. In addition, application of Freundlich, Langmuir, Dubinin-Radushkevhch, and Tempkin models and pseudo-first-order and pseudo-second-order adsorption synthetic equations aimed at determining the type of adsorbent isotherm. The results showed that the best fitting of Langmuir (R2 = 0.9944, qm = 400 mg/g) for adsorption isotherm and pseudo-second-order model (R2 = 0.9905) for kinetics studies. Furthermore, data of Gibbs free energy and enthalpy demonstrated an exothermic and spontaneous process in the research.


Assuntos
Poluentes Químicos da Água , Água , Adsorção , Cinética , Soluções , Concentração de Íons de Hidrogênio , Termodinâmica , Água/química , Espectroscopia de Infravermelho com Transformada de Fourier , Poluentes Químicos da Água/química , Indicadores e Reagentes , Penicilina G
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 284: 121756, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36088740

RESUMO

The growing appearance of antibiotic-resistant strains of microorganisms originated from the widespread use and ubiquitous presence of such drugs is a major concern in the world. The development of methodologies able to detect such substances at low concentration in real water samples is mandatory to overcome this problem. Europium(III) is known to form complexes with tetracycline (TC) with photoluminescent characteristics useful for TC determination. In the present work, we synthesized for the first time carbon nanoparticles (CN) showing delayed photoluminescence using a Europium(III) doping synthesis. The new material (PCNEu) was characterized both morphologically and spectroscopically, showing an analytical photoluminescent signal in presence of TC, arising from the 5D0→7F2 transition of europium, one hundred times higher than that of the europium salt alone in presence of the antibiotic. This enhancement is a consequence of the amplifying effect exerted by nanoparticle structure itself, leading to an efficient synergistic "antenna effect" in the system PCNEu - TC. The analytical signal is affected both by pH and the nature of the buffer used, and it allows the detection of tetracycline in waters with a limit of detection of 2.18 nM and recoveries between 90 and 110%. The analytical performance of the developed methodology enables having lower limits of detection than other luminescent and chemiluminescent reported methodologies.


Assuntos
Compostos Heterocíclicos , Nanopartículas , Antibacterianos/química , Carbono/química , Európio/química , Indicadores e Reagentes , Nanopartículas/química , Tetraciclina/química , Água
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 286: 121904, 2023 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-36228490

RESUMO

Calycosin, the major bioactive isoflavonoid inAstragali Radix and an important anti-viral drug with a variety of pharmacological actions, is being determined by five different spectroscopic methods. Two spectrophotometric methods have been investigated including measuring the absorption spectra at λmax = 270 nm and the first derivative spectra at λ = 288 nm for methods I and II, respectively. For the first time; the native fluorescence of calycosin is measured without adding any reagents. The fluorescence intensity was measured at 340 nm after excitation at 282 nm in method III. The fourth method involves the direct measuring of a first derivative spectrofluorimetric emission peak at 292 nm. In method V synchronous fluorescence spectra were recorded in methanol at Δλ = 70 nm. The linear range for the fluorescence-based methods was 0.05-1.0 µg/mL and for the UV-based methods was 0.5-10.0 µg/mL. The methods were validated per International Council of Harmonization (ICHQ2R1) guidelines. The limits of detection were found to be down to 0.11 and 0.12 µg/mL for the spectrophotometric methods, and 15.0, 18.0,16.0 ng/ mL, for the spectrofluorimetric approaches respectively, representing the high sensitivity. Accordingly, this permitted the quantitation of calycosin in spiked human plasma samples with satisfactory percentage recoveries (94.50.-102.50 %). The methods were utilized for calycosin analysis in different matrices including plasma and capsules with high precision and accuracy.


Assuntos
Isoflavonas , Humanos , Espectrometria de Fluorescência/métodos , Cápsulas , Indicadores e Reagentes
7.
Life Sci Alliance ; 6(1)2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36368907

RESUMO

The modification of substrates with ADP-ribose (ADPr) is important in, for example, antiviral immunity and cancer. Recently, several reagents were developed to detect ADP-ribosylation; however, it is unknown whether they recognise ADPr, specific amino acid-ADPr linkages, or ADPr with the surrounding protein backbone. We first optimised methods to prepare extracts containing ADPr-proteins and observe that depending on the amino acid modified, the modification is heatlabile. We tested the reactivity of available reagents with diverse ADP-ribosylated protein and RNA substrates and observed that not all reagents are equally suited for all substrates. Next, we determined cross-reactivity with adenylylated RNA, AMPylated proteins, and metabolites, including NADH, which are detected by some reagents. Lastly, we analysed ADP-ribosylation using confocal microscopy, where depending on the fixation method, either mitochondrion, nucleus, or nucleolus is stained. This study allows future work dissecting the function of ADP-ribosylation in cells, both on protein and on RNA substrates, as we optimised sample preparation methods and have defined the reagents suitable for specific methods and substrates.


Assuntos
ADP-Ribosilação , RNA , RNA/metabolismo , Indicadores e Reagentes , Adenosina Difosfato Ribose/química , Adenosina Difosfato Ribose/metabolismo , Proteínas/metabolismo , Aminoácidos/metabolismo
8.
J Infect Chemother ; 29(1): 115-117, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36183991

RESUMO

TRCReady® SARS-CoV-2 i is a reagent for transcription-reverse transcription concerted reaction (TRC) to detect SARS-CoV-2 N2 gene, used with the automated rapid isothermal nucleic acid amplification test (NAAT) analyzer TRCReady®-80. Sensitivity and specificity of TRCReady® SARS-CoV-2 i was assessed by comparison with the results of real-time reverse transcription-polymerase chain reaction (RT-PCR) using nasopharyngeal swab samples. From November 2020 to March 2021, a total of 441 nasopharyngeal swabs were obtained and analyzed both with TRCReady® SARS-CoV-2 i and RT-PCR. Sensitivity and specificity of TRCReady® SARS-CoV-2 i were 94.6% (53/56) and 99.2% (382/385), respectively. Reaction time to positivity of TRCReady® SARS-CoV-2 i ranged from 1.166 to 9.805 (median: 2.887) min, and minimum detection sensitivity of TRCReady® SARS-CoV-2 i was 9 copies per test, with reaction time as 5.014 min. Detection of SARS-CoV-2 gene from nasopharyngeal swab sample using TRCReady® SARS-CoV-2 i shows comparative diagnostic test accuracy with RT-PCR, and can be used as a useful test to diagnose SARS-CoV-2 infection.


Assuntos
COVID-19 , SARS-CoV-2 , Humanos , SARS-CoV-2/genética , COVID-19/diagnóstico , Transcrição Reversa , Indicadores e Reagentes , Testes Diagnósticos de Rotina , Sensibilidade e Especificidade , Nasofaringe
9.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121918, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36179569

RESUMO

An easy and reliable method is optimized for extractive spectrophotometric assessment of platinum(IV) with 4-(4'-nitrobenzylideneimino)-3-methyl-5-mercapto-1,2,4-triazole as an extractant. The basis of this method is the formation of red platinum(IV) complex with the above reagent in acetate buffer medium (pH 5.0) and extraction in chloroform. Good linearity with regression equation as y = 1.011 × 104 ×  + 0.002 having correlation coefficient (R2) of 0.998 over concentration up to 17.5 µg mL-1 of platinum(IV) was achieved with apparent molar absorptivity of 1.011 × 104 L mol-1 cm-1. The limit of detection (0.22 µg mL-1), limit of quantification (0.73 µg mL-1) and Sandell's sensitivity (0.0193 µg cm-2) were also estimated. The interference of various cations was removed by using proper masking agents and consequently by using EDTA and citrate to mask certain transition metals, the method becomes highly specific for platinum(IV), including the effects of platinum group metals. The method is effectively used for determination of platinum(IV) from environmental and real samples such as alloys, catalysts, thermocouple wire and pharmaceutical sample.


Assuntos
Cisplatino , Platina , Espectrofotometria/métodos , Ligas , Indicadores e Reagentes
10.
Talanta ; 252: 123803, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35988300

RESUMO

Derivatization reagents based on azobenzene containing different N-hydroxysuccinimidyl moieties- AzoB (carbamate) and AzoC (ester) - are proposed for the LC-ESI-MS analysis of free amino acids in fermented beverages and juices. A dual comparison between LC-MS/MS in positive and negative ESI modes in dynamic Multiple Reaction Monitoring (dMRM) and Neutral Loss Scan was investigated. The results indicate that the studied carbamate derivatization reagent, AzoB, can be employed for targeted analysis (MS/MS) but also for non-targeted analysis of derivatized amino acids thanks to its constant neutral loss (223 Da) that is the same in both ionisation modes. For amines, precursor ion scan can be used as identification tool. The derivatization properties of AzoB and AzoC were compared against other derivatization reagents, and they showed advantages such as fast derivatization reaction and good reactivity with secondary amines. AzoC also displayed a disadvantage -side products were formed that affect the quantitation. Free amino acids profile of Kvass (a fermented beverage from eastern Europe) was determined for first time, proline was found to be the most abundant amino acid.


Assuntos
Aminoácidos , Espectrometria de Massas em Tandem , Cromatografia Líquida/métodos , Espectrometria de Massas em Tandem/métodos , Aminoácidos/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Indicadores e Reagentes , Aminas/química , Carbamatos/química
11.
Talanta ; 252: 123730, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36030735

RESUMO

In this paper, we report about the application of a sensitive fluorescent derivatization reagent Coumarin151-N-Hydroxysuccinimidyl Carbamate (Cou151DSC) for amino compounds using high-performance liquid chromatography (HPLC) compatible with ultraviolet (UV), fluorescence detector (FLD) and electrospray ionization - tandem mass spectrometry (ESI-MS/MS)-positive mode. We optimized derivatization procedure and validated an analytical method to determine 24 amino acids in Kvass drink using Norvaline as an internal standard. Compared to 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate (6-AQC), the derivatization with Cou151 DSC is faster and milder, for 5 min at 40°C instead of 15 min at 55°C. The limit of quantitation (LOQ, pmol on column) for 21 amino acids in this work is lower 1.1-30.0 times than values obtained with 6-AQC. The derivatives have excitation wavelength at 355 nm and emission wavelength at 486 nm. Their MS/MS fragmentation behaviors were examined together with 23 other amino compounds. We found three possibilities to lose a neutral group which can be Coumarin 151 isocyanate Cou151NCO (255 Da), amine Coumarin 151 (229 Da) or urea Cou151CONH2 (272 Da). The accuracy of the proposed method was within 83-107% with good relative standard deviations (RSDs) of equal or less than 6%. The recoveries were from 82 to 120% in four spiked concentrations, repeatability was between 0 and 14%. The intra- and inter-day precision are less than 13% and 18%, respectively.


Assuntos
Aminoácidos , Espectrometria de Massas em Tandem , Cromatografia Líquida/métodos , Aminoácidos/análise , Indicadores e Reagentes , Cromatografia Líquida de Alta Pressão/métodos , Aminas , Cumarínicos/análise
13.
Artigo em Inglês | MEDLINE | ID: mdl-36455389

RESUMO

The esters of endogenous anabolic steroids are the most frequently used doping agents for prolonging the half-life of exogenously ingested endogenous anabolic steroids. As a cost- and time-saving matrix, dried blood spots (DBSs) are valuable for directly detecting endogenous anabolic steroid esters in blood and for providing conclusive evidence of their abuse. In this study, a method for simultaneous detection of 20 endogenous anabolic steroid esters in DBSs based on ultra-high performance liquid chromatography-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap-MS) with parallel reaction monitoring (PRM) was developed and validated, and 10 of these esters were analyzed in DBSs for the first time. This method analyzes the greatest number and types of endogenous anabolic steroid esters of any current method using DBSs. Girard's Reagent P (GRP) was used for the derivatization of endogenous anabolic steroid esters in a DBS matrix for the first time, and the conditions of the derivatization reaction were optimized to achieve a higher sensitivity compared to previous methods. Selectivity, limit of detection (LOD), extraction recovery, precision (intra- and inter-), matrix effects, and carry-over were analyzed to validate the method. The LODs were lower and the recoveries were higher than those of previous studies. The relative standard deviation of the intraday precision was below 20% and the interday precision was below 35%. A product ion analysis of GRP nandrolone ester, GRP boldenone ester, GRP dehydroepiandrosterone acetate, and GRP androstanolone ester derivatives was performed, and the structures of the fragment ions were proposed for the first time.


Assuntos
Di-Hidrotestosterona , Ésteres , Indicadores e Reagentes , Cromatografia Líquida de Alta Pressão , Meia-Vida
14.
J. Health Biol. Sci. (Online) ; 10(1): 1-8, 01/jan./2022. tab, graf, ilus
Artigo em Português | LILACS | ID: biblio-1378478

RESUMO

Objetivos: descrever os aspectos epidemiológicos, espaciais e temporais da leishmaniose visceral humana, no município de Sobral, no período de 2007 a 2019. Métodos: foi realizado um estudo epidemiológico descritivo e ecológico de análise espacial e temporal, com uso dos programas Quantum-Gis e Joinpoint. Resultados: foram confirmados 316 casos novos, predominantemente, no sexo masculino, nas faixas etárias de 1 a 4 anos (26,3%) e de 20 a 39 anos (24,0%), na zona urbana. Febre (95,9%), fraqueza (85,1%), emagrecimento (80,1%), palidez (73,7%), esplenomegalia (75,6%) e hepatomegalia (69,3%) foram os sinais clínicos mais frequentes. A doença se concentrou em quatro bairros: Terrenos Novos, Centro, Expectativa e Sinhá Saboia, expressando áreas mais densas (quentes). A incidência e a letalidade foram crescentes no início do período e decrescentes no final, com uma inversão destes indicadores no ano de 2014. Conclusão: em Sobral, a leishmaniose visceral humana é um agravo considerado relevante para o serviço de vigilância em saúde com necessidade de intensificação das ações de controle entomológico, zoonótico e de manejo ambiental, principalmente nas áreas mais densas.


Objectives: the objective was to describe the epidemiological, spatial, and temporal aspects of human visceral leishmaniasis, in the municipality of Sobral, from 2007 to 2019. Methods: A descriptive, spatial and temporal ecological study was carried out using Quantum-Gis and Joinpoint programs. Results: 316 new cases were confirmed, predominantly in males, in the age groups of 1 to 4 years (26.3%) and 20 to 39 years (24.0%), mainly in the urban area. Fever (95.9%), weakness (85.1%), weight loss (80.1%), pallor (73.7%), splenomegaly (75.6%), and hepatomegaly (69.3%) were the most frequent clinical signs. The disease was concentrated in four neighborhoods (Terrenos Novos, Centro, Expectativa, and Sinhá Saboia), hot spots. The incidence and case-fatality increased at the beginning of the period but decreased at the end, with an inversion in these in the year 2014. Conclusion: In Sobral, human visceral leishmaniasis is a disease considered relevant to the health surveillance service, with the need to intensify entomological, zoonotic, and environmental management actions, especially in denser areas.


Assuntos
Leishmaniose Visceral , Sinais e Sintomas , Vigilância Sanitária , Epidemiologia , Mortalidade , Indicadores e Reagentes , Grupos Etários
16.
J Org Chem ; 87(23): 16090-16098, 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36370090

RESUMO

We report herein a facile strategy to synthesize trifluoromethylated γ-lactams through trifluoromethylcarbonylation of N-cyano alkenes using readily available CF3SO2Na as the CF3 radical source. A range of trifluoromethyl-containing γ-lactams was obtained in good yields. This transition-metal-free protocol is demonstrated with mild conditions, broad substrate scope, good functional group tolerance, convenient reagents, and an easy-to-handle operating system.


Assuntos
Alcenos , Lactamas , Indicadores e Reagentes , Metilação , Íons
17.
Anal Chim Acta ; 1236: 340596, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36396227

RESUMO

The increasing consumption of processed foods demands the usage of chemical preservatives to ensure freshness and extended shelf life. For this purpose, sodium sulfite and its derivatives have been widely used in a variety of food products to inhibit microbial spoilage and for mitigating oxidative decay. However, the excessive consumption of sulfite may cause health problems, thus requiring rapid and accurate analytical methods for the rapid identification of threshold levels. Conventionally, sulfite is volatilized from food samples by acidification followed by trapping of the gaseous SO2 and determination using a suitable analytical technique. Herein, we propose a yet unprecedented reagent-less approach via direct absorbance measurements of gaseous SO2 at 280 nm after sample acidification. The detection system combines a deep-UV LED and a SiC photodiode with a substrate-integrated hollow waveguide (iHWG) gas cell. Absorbance measurements were performed using a log-ratio amplifier circuitry, resulting in noise levels <0.7 mAU. This innovative concept enabled the determination of sulfite in beverages in the range of 25-1000 mg L-1 with suitable linearity (r2 > 0.99) and an analysis time <30 s. The limit of detection (LOD) was calculated at 14.3 mg L-1 (3σ) with an iHWG providing an optical path length of 75 mm. As a proof of concept, this innovative analytical platform was employed for sulfite quantification in concentrated grape juice, coconut water and beer, with suitable accuracy in terms of recovery (83-117%) and favorable comparison with the official Monier-Williams method. Given the inherent modularity and adaptability of the device concept, we anticipate the application of the proposed analytical platform for the in-situ studies addressing sulfite and other volatilized preservatives in a wide variety of food products with tailorable detectability.


Assuntos
Análise de Alimentos , Sulfitos , Indicadores e Reagentes , Sulfitos/análise , Fenômenos Químicos , Bebidas/análise
18.
J Chromatogr A ; 1685: 463622, 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36375215

RESUMO

Efficient extraction is a vital step in mercury speciation. In this context, using 4-vinylbenzeneboronic acid and 9-vinylanthracene as functional monomers, a new magnetic adsorbent was fabricated according to one-pot hydrothermal approach. Various characterization results prove the as-prepared adsorbent presented abundant functional groups and saturation magnetism. Combining with magnetic solid phase extraction (MSPE), the adsorbent exhibited satisfactory entrapment performance towards different mercury species which had been pre-coordinated with dithizone to form metal-organic coordination. A series of parameters influencing the extraction performance were inspected in detail. Under the most beneficial conditions, sensitive and reliable approach to quantify trace methylmercury, ethylmercury, phenylmercury and inorganic mercury in aqueous samples was developed by the combination of HPLC/DAD. Limits of detection and precision located in the ranges of 0.012-0.074 µg/L and 2.5-9.8%, respectively. Recoveries with low, medium and high fortified contents in actual waters varied from 79.8 to 119%. Confirmatory experiments were performed to evidence the accuracy and reliability of established approach. In addition, a possible mechanism was suggested based on the chemical nature of analytes, extraction conditions and characterization results.


Assuntos
Mercúrio , Mercúrio/análise , Cromatografia Líquida de Alta Pressão/métodos , Reprodutibilidade dos Testes , Extração em Fase Sólida/métodos , Água/química , Indicadores e Reagentes/análise , Fenômenos Magnéticos
19.
Klin Lab Diagn ; 67(11): 663-667, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36398776

RESUMO

The coronavirus infection continues to spread around the world. In this regard, the purpose of this work was: to develop a set of reagents for the qualitative detection of SARS-CoV-2 virus RNA. The set was developed by CJSC «Ecolab¼, 20 positive samples were used to develop the kit. The research method consisted of several stages: isolation of SARS-CoV-2 RNA, RNA reverse transcription reaction and PCR amplification of cDNA with simultaneous detection of the result in real time. The main characteristics of the kit: analytical sensitivity - 100%, specificity - 100%, accuracy - 100%. Thus, our method for diagnosing a new coronavirus infection based on real-time RT-PCR makes it possible to qualitatively and quickly detect betacoronavirus RNA in clinical material from patients and healthy individuals with suspected coronavirus infection and other symptoms of SARS.


Assuntos
COVID-19 , SARS-CoV-2 , Humanos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , SARS-CoV-2/genética , RNA Viral/análise , Indicadores e Reagentes , Sensibilidade e Especificidade , COVID-19/diagnóstico , Reação em Cadeia da Polimerase em Tempo Real
20.
Org Lett ; 24(46): 8578-8583, 2022 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-36382857

RESUMO

The straightforward and rapid incorporation of a thiazolidinedione scaffold into prefunctionalized (hetero)aromatic compounds is in demand for the development of antidiabetic glitazones and other pharmaceuticals. Herein, we report the unprecedented N- and O-directed C-H alkylation of various (hetero)arenes with methylene thiazolidinediones under rhodium(III) catalysis. The applicability of the developed protocol in challenging contexts is exhibited by the late-stage installation of a methylene thiazolidinedione moiety on the C-H bond of commercially available drug molecules. Combined mechanistic investigations aided the elucidation of a plausible reaction mechanism.


Assuntos
Tiazolidinedionas , Indicadores e Reagentes , Catálise , Alquilação
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