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2.
Gen Comp Endocrinol ; 285: 113275, 2020 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-31536721

RESUMO

The progestin receptor membrane components (Pgrmcs) contain two paralogs, Pgrmc1 and Pgrmc2. Our previous research into single knockout of Pgrmc1 or Pgrmc2 suggests that Pgrmc1 and Pgrmc2 regulate membrane progestin receptor or steroid synthesis and therefore female fertility in zebrafish. Additional roles of Pgrmcs may not be determined in using single Pgrmc knockouts due to compensatory roles between Pgrmc1 and Pgrmc2. To address this question, we crossed single knockout pgrmc1 (pgrmc1-/-) with pgrmc2 (pgrmc2-/-), and generated double knockouts for both pgrmc1 and pgrmc2 (pgrmc1/2-/-) in a vertebrate model, zebrafish. In addition to the delayed oocyte maturation and reduced female fertility, significant reduced ovulation was found in double knockout (pgrmc1/2-/-) in vivo, though not detected in either single knockout of Pgrmc (pgrmc1-/- or pgrmc2-/-). We also found significant down regulation of nuclear progestin receptor (Pgr) protein expression only in pgrmc1/2-/-, which was most likely the cause of reduced ovulation. Lower protein expression of Pgr also resulted in reduced expression of metalloproteinase in pgrmc1/2-/-. With this study, we have provided new evidence for the physiological functions of Pgrmcs in the regulation of female fertility by regulation of ovulation, likely via regulation of Pgr, which affects regulation of metalloproteinase expression and oocyte ovulation.


Assuntos
Núcleo Celular/metabolismo , Regulação para Baixo , Técnicas de Inativação de Genes , Infertilidade/genética , Proteínas de Membrana/deficiência , Receptores de Progesterona/genética , Proteínas de Peixe-Zebra/deficiência , Peixe-Zebra/genética , Animais , Feminino , Proteínas de Membrana/metabolismo , Metaloproteases/metabolismo , Oócitos/metabolismo , Oogênese , Ovário/metabolismo , Ovulação , Receptores de Progesterona/deficiência , Receptores de Progesterona/metabolismo , Peixe-Zebra/metabolismo , Proteínas de Peixe-Zebra/metabolismo
4.
J Ethnopharmacol ; 247: 112269, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31610261

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Cisplatin (CP) is the classical chemotherapeutic drug for various cancer, but it also accompanies reproductive toxicity and genotoxicity. Liuwei Dihuang Pill (LW) is the traditional Chinese medicine prescription for treating Kidney-Yin deficiency syndrome, which has been reported to prevent and treat various diseases. However, the protective effect of LW on CP-induced reproductive toxicity and genotoxicity has not been reported. AIM OF THE STUDY: To investigate the potential protective effect and mechanism of LW on CP-induced reproductive toxicity and genotoxicity in male mice. MATERIALS AND METHODS: Mice were given LW (0.4, 1.2 and 3.6 g/kg) or Vitamin C (0.1 g/kg) once daily by oral gavage for thirteen consecutive days. Then, CP (3.00 mg/kg) was given intraperitoneal injection once daily for five consecutive days starting on the ninth day. The protective effects of LW against CP-induced reproductive toxicity and genotoxicity were evaluated by body weight, testis ratio, sperm count, sperm viability, sperm abnormal morphology type, micronuclei test, testicular histopathology, serum malondialdehyde (MDA), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) level. RESULTS: The results demonstrated that LW could significantly increase CP-induced the reduction of sperm count and sperm viability, then decrease abnormal sperm type rate and micronucleus rate. Moreover, LW also could improve testicular abnormal histopathologic morphology induced by CP exposure. Meanwhile, LW decreased serum MDA level and increased T-SOD, GSH-Px and CAT level compared to CP group. CONCLUSION: our findings show that LW has protective effects on CP-induced reproductive toxicity and genotoxicity. LW decreases serum MDA level and increases T-SOD, GSH-Px and CAT level, which indicates that antioxidant activity may be the potential mechanism of LW to resist reproductive toxicity and genotoxicity.


Assuntos
Antioxidantes/farmacologia , Cisplatino/efeitos adversos , Medicamentos de Ervas Chinesas/farmacologia , Substâncias Protetoras/farmacologia , Animais , Dano ao DNA/efeitos dos fármacos , Humanos , Infertilidade/induzido quimicamente , Infertilidade/prevenção & controle , Masculino , Camundongos , Testes para Micronúcleos , Modelos Animais , Estresse Oxidativo/efeitos dos fármacos , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Espermatozoides/patologia , Testículo/efeitos dos fármacos , Testículo/patologia
5.
BJOG ; 127(2): 208-216, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31529767

RESUMO

OBJECTIVE: The objective of this study was to use high-throughput sequencing to describe the vaginal eukaryotic DNA virome in patients undergoing in vitro fertilisation (IVF) to examine associations between the vaginal virome, antibiotic exposure and IVF outcomes. DESIGN: Prospective exploratory study. SETTING: Single academic fertility centre. POPULATION: Subfertile women age 18-43 years undergoing their first IVF cycle with a fresh embryo transfer. METHODS: The primary exposure was prophylactic azithromycin or no azithromycin before IVF. A mid-vaginal swab was obtained at the time of embryo transfer for virome analysis. MAIN OUTCOME MEASURES: The primary outcomes compared between exposure groups were characteristics of vaginal virome and clinical pregnancy rates. Secondary outcomes were virome associations with number of oocytes retrieved, number of blastocysts and implantation rate. RESULTS: Twenty-six women contributed a vaginal swab before embryo transfer. There were no significant differences in IVF outcomes between azithromycin groups. There was no association between viral diversity and clinical pregnancy overall. A higher diversity of herpesviruses and α-papillomaviruses was observed in samples from the azithromycin-treated group compared with the no azithromycin group (P = 0.04). In women that received azithromycin, viral diversity was higher in the group that did not achieve clinical pregnancy compared with those who did (P = 0.06). CONCLUSIONS: We demonstrate that the vaginal eukaryotic virome in women undergoing IVF is associated with antibiotic exposure. Additionally, we demonstrate an inverse trend between viral diversity and pregnancy, with a higher number of viruses detected associated with failure to achieve clinical pregnancy in the azithromycin group. TWEETABLE ABSTRACT: Higher viral diversity is associated with prophylactic antibiotic exposure in subfertile women undergoing IVF.


Assuntos
Eucariotos/fisiologia , Fertilização In Vitro , Infertilidade/terapia , Microbiota , Vagina/virologia , Adulto , Antibacterianos/uso terapêutico , DNA Viral/fisiologia , Transferência Embrionária , Feminino , Herpesviridae , Humanos , Microbiota/genética , Microbiota/imunologia , Papillomaviridae , Gravidez , Estudos Prospectivos , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/imunologia , Análise de Sequência de DNA , Vagina/microbiologia
7.
Nature ; 575(7781): 55-56, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31680127
8.
J S Afr Vet Assoc ; 90(0): e1-e7, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31714112

RESUMO

Calving rate in communal cattle influences both food security and socio-economics in rural households. A previous study indicated that scrotal damage caused by ticks could affect the fertility of communal bulls and reduce the annual calving rate. The objectives of the study were to investigate the annual calving rate in communal herds by counting calves during herd visits, perform breeding soundness examinations on bulls and identify adult ticks attached to their genitalia. This prospective longitudinal survey was based on participatory rural appraisal. Calving rates were estimated in cows (n = 2398) from 100 randomly selected communal herds in Moretele over 12 months in 2013, during routine visits by animal health technicians. Randomly selected bulls (n = 50) from these herds were tested for Brucella abortus, Trichomonas foetus and Campylobacter fetus subspecies venerealis. The calving rate was 35.86% (0.359). The mean scrotal circumference was 37.63 ± 3.42 cm. Total sperm motility was 78.73 ± 35.73%; progressive sperm motility was 27.39 ± 15.81% and non-progressive sperm motility was 51.34 ± 19.92%. Thirty-five of the 38 bulls examined for breeding soundness exhibited severe scrotal and preputial lesions caused by the adult ticks Amblyomma hebraeum and Hyalomma rufipes. Tick control methods used included spraying (n = 20), pour-on (n = 11), no control (n = 1) and various (n = 18). It was concluded that in Moretele genital tick damage had a more serious impact on the fertility of communal bulls than contagious diseases. Targeted acaricidal spot treatment of the genitalia of communal bulls to prevent infestation is recommended, as tick control strategies used by farmers appeared to be inadequate.


Assuntos
Doenças dos Bovinos/parasitologia , Infertilidade/veterinária , Doenças Transmitidas por Carrapatos/veterinária , Criação de Animais Domésticos , Animais , Coeficiente de Natalidade , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/prevenção & controle , Feminino , Infertilidade/epidemiologia , Infertilidade/parasitologia , Estudos Longitudinais , Masculino , Fatores de Risco , Escroto/parasitologia , África do Sul/epidemiologia , Doenças Transmitidas por Carrapatos/complicações , Doenças Transmitidas por Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/prevenção & controle , Carrapatos/parasitologia
9.
Adv Exp Med Biol ; 1206: 453-468, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31776998

RESUMO

Autophagy, a major degradation/recycling pathway, plays an essential role in cellular homeostasis maintenance, cell fate decision, and reproductive development. During reproduction, sperms and eggs, the specialized haploid gametes produced by the meiotic process of the germ cells in male and female respectively, are fused to form a new zygote that develops into fetus through embryogenesis and maternal-fetal crosstalk. Researches carried out in the past few years have proved that autophagy plays a key role in the regulation of reproduction process, and blockage of autophagy process likely contributes to reproductive abnormalities and even infertility. Here we summerize the recent progress in exploring the functional roles of autophagy in reproductive processes, such as spermatogenesis, folliculogenesis, fertilization, embryogenesis, and maternal-fetal crosstalk, in both animals and plants.


Assuntos
Autofagia , Reprodução , Animais , Feminino , Humanos , Infertilidade , Masculino , Reprodução/fisiologia , Espermatogênese/fisiologia , Espermatozoides
10.
BMC Public Health ; 19(1): 1548, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31752817

RESUMO

BACKGROUND: Research highlighted that Stressful Life Events have high incidence among infertile patients and significant impact on physical and medical parameters related to reproductive functions, but their potential role among factors influencing the infertile patients' perception of fertility-related Quality of Life (QoL) has not been explored. The present study aims to investigate the associations of Stressful Life Events (Stressful events in the family of origin, In family pre-existing pregnancy difficulties, Health problems in childhood) with perceived fertility-related QoL in women attending infertility treatments, examining the potential moderating role of adopted coping strategies and perceived couple's dyadic adjustment. METHODS: A questionnaire consisting of Socio-demographics and Infertility-related characteristics, Stress-inducing events in the couples' lives Questionnaire (FLS), Coping Orientations to Problem Experienced (COPE), Dyadic Adjustment Scale (DAS), and Core and Treatment subscales of Fertility Quality of Life (FertiQoL) was administered to 266 women attending infertility treatments. A descriptive correlational design with cross-sectional comparison was used. Results Logistic Regression Analyses after adjusting for socio-demographic and infertility-related characteristics revealed that women who reported Stressful events in the family of origin and In family pre-existing pregnancy difficulties were more likely to report lower levels of perceived Core QoL, while women who reported Health problems in childhood were more likely to report lower levels of perceived Treatment QoL. Couple's dyadic adjustment and specific coping strategies were significantly associated with perceived Core and Treatment QoL and they also significantly moderated the associations between stressful life events and perceived QoL. CONCLUSIONS: Data provided original evidence on the strong association between stressful life events and perceived fertility-related QoL also highlighting individual and couples' resources to define counselling interventions with women attending infertility treatments.


Assuntos
Adaptação Psicológica , Infertilidade/psicologia , Infertilidade/terapia , Relações Interpessoais , Qualidade de Vida/psicologia , Cônjuges/psicologia , Estresse Psicológico/psicologia , Adulto , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cônjuges/estatística & dados numéricos , Inquéritos e Questionários , Adulto Jovem
11.
Soins Pediatr Pueric ; 40(311): 12-17, 2019 Nov.
Artigo em Francês | MEDLINE | ID: mdl-31757269

RESUMO

Medically assisted reproduction techniques are aimed at heterosexual couples, when both members are living, of reproductive age, consenting to acts of insemination or the transfer of embryos and presenting medically diagnosed infertility. The review of the law on bioethics could offer new perspectives for reproduction notably by allowing access to medically assisted reproduction (MAR) to all women, elective egg freezing, the lifting of gamete donor anonymity and the authorisation of post-mortem MAR.


Assuntos
Bioética , Infertilidade , Técnicas de Reprodução Assistida , Feminino , Humanos , Técnicas de Reprodução Assistida/ética
12.
Soins Pediatr Pueric ; 40(311): 37-39, 2019 Nov.
Artigo em Francês | MEDLINE | ID: mdl-31757275

RESUMO

Following a diagnosis of infertility, Claire and Michael decided to explore the option of medically assisted reproduction. They share their journey, their experience and their suffering on this long and difficult pathway towards pregnancy.


Assuntos
Infertilidade , Técnicas de Reprodução Assistida , Feminino , Humanos , Gravidez
13.
14.
Rev Med Suisse ; 15(668): 1926-1931, 2019 Oct 23.
Artigo em Francês | MEDLINE | ID: mdl-31643153

RESUMO

Chlamydia trachomatis (CT) infection is the most frequent notifiable sexually transmitted infection (STI) in Switzerland. The infection is most frequently observed in 15 to 24 year-old-women and in 25 to 34 year-old-men. 50-75 % of the Chlamydia trachomatis carriage are asymptomatic, making the infection difficult to diagnose and increasing the untreated specimen, leading to complications like infertility, ectopic pregnancy or pelvic inflammatory disease. Despite having a sexual prevention at school, the youths seem to have a lack of knowledge about CT, her transmission and her complications. We performed a survey, which showed that 60.5 % of the participants ignored that this bacteria is mostly asymptomatic. We also found that 11 % of the participants believed that there is no possible relapse of the infection. The prevention must be strengthened, mostly because there is no program in Switzerland, letting every physician to his own beliefs. The medical consultation is an ideal opportunity for this prevention and the youths shared their wish to discuss more about it with health professionals.


Assuntos
Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/microbiologia , Chlamydia trachomatis , Metas , Educação em Saúde , Adolescente , Adulto , Doenças Assintomáticas/epidemiologia , Erradicação de Doenças , Feminino , Humanos , Infertilidade/epidemiologia , Infertilidade/microbiologia , Masculino , Doença Inflamatória Pélvica/epidemiologia , Doença Inflamatória Pélvica/microbiologia , Gravidez , Suíça/epidemiologia , Adulto Jovem
15.
Nat Commun ; 10(1): 4626, 2019 10 11.
Artigo em Inglês | MEDLINE | ID: mdl-31604923

RESUMO

Infertility in men and women is a complex genetic trait with shared biological bases between the sexes. Here, we perform a series of rare variant analyses across 73,185 women and men to identify genes that contribute to primary gonadal dysfunction. We report CSMD1, a complement regulatory protein on chromosome 8p23, as a strong candidate locus in both sexes. We show that CSMD1 is enriched at the germ-cell/somatic-cell interface in both male and female gonads. Csmd1-knockout males show increased rates of infertility with significantly increased complement C3 protein deposition in the testes, accompanied by severe histological degeneration. Knockout females show significant reduction in ovarian quality and breeding success, as well as mammary branching impairment. Double knockout of Csmd1 and C3 causes non-additive reduction in breeding success, suggesting that CSMD1 and the complement pathway play an important role in the normal postnatal development of the gonads in both sexes.


Assuntos
Infertilidade/genética , Proteínas de Membrana/genética , Proteínas Supressoras de Tumor/genética , Fatores Etários , Animais , Complemento C3/metabolismo , Feminino , Estudos de Associação Genética , Genótipo , Humanos , Masculino , Glândulas Mamárias Animais/crescimento & desenvolvimento , Glândulas Mamárias Animais/patologia , Menopausa/genética , Camundongos Knockout , Mutação , Ovário/patologia , Maturidade Sexual , Testículo/metabolismo
16.
Rinsho Ketsueki ; 60(9): 1378-1385, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31597867

RESUMO

The frequency of hematological malignancies is quite high in children, adolescents and young adults. Infertility after treatment is an important issue affecting the quality of life of long-term survivorsas the outcome of treatment is improved. Recently, several guidelines for infertility and fertility preservation have been published. Consequently, it has become easier to obtain information on the risk of infertility and fertility preservation therapy for each treatment. However, the information on the optimal timing for fertility preservation and current outcomes of assisted reproductive technology using stored oocytes, embryos, ovarian tissues, or sperm remains limited. Further, whether fertility preservation while using a new drug with unknown risk for infertility should be performed remains an unresolved issue.


Assuntos
Preservação da Fertilidade , Neoplasias Hematológicas/complicações , Neoplasias Hematológicas/terapia , Infertilidade/prevenção & controle , Adolescente , Criança , Criopreservação , Humanos , Qualidade de Vida , Adulto Jovem
17.
PLoS Genet ; 15(10): e1008409, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31589614

RESUMO

Limited translational genomic research data have been reported on the application of exome sequencing and parallel gene testing for preconception carrier screening (PCS). Here, we present individual-level data from a large PCS program in which exome sequencing was routinely performed on either gamete donors (5,845) or infertile patients (8,280) undergoing in vitro fertilization (IVF) treatment without any known family history of inheritable genetic conditions. Individual-level data on pathogenic variants were used to define conditions for PCS based on criteria for severity, penetrance, inheritance pattern, and age of onset. Fetal risk was defined based on actual carrier frequency data accounting for the specific inheritance pattern (fetal disease risk, FDR). In addition, large-scale application of exome sequencing for PCS allowed a deep investigation of the incidence of medically actionable secondary findings in this population. Exome sequencing achieved remarkable clinical sensitivity for reproductive risk of highly penetrant childhood-onset disorders (1/337 conceptions) through analysis of 114 selected gene-condition pairs. A significant contribution to fetal disease risk was observed for rare (carrier rate < 1:100) and X-linked conditions (16.7% and 41.2% of total FDR, respectively). Subgroup analysis of 776 IVF couples identified 37 at increased reproductive risk (4.8%; 95% CI = 3.4-6.5). Further, two additional couples had increased risk for very rare conditions when both members of a parental pair were treated as a unit and the search was extended to the entire exome. About 2.3% of participants showed at least one pathogenic variant for genes included in the updated American College of Medical Genetics and Genomics v2.0 list of secondary findings. Gamete donors and IVF couples showed similar carrier burden for both carrier screening and secondary findings, indicating no causal relationship to fertility. These translational research data will facilitate development of more effective PCS strategies that maximize clinical sensitivity with minimal counterproductive effects.


Assuntos
Genes Recessivos , Triagem de Portadores Genéticos , Predisposição Genética para Doença , Infertilidade/genética , Adulto , Criança , Pré-Escolar , Doação Dirigida de Tecido , Exoma/genética , Feminino , Testes Genéticos , Genoma Humano/genética , Genômica , Heterozigoto , Humanos , Lactente , Recém-Nascido , Infertilidade/epidemiologia , Infertilidade/fisiopatologia , Masculino , Mutação/genética , Pesquisa Médica Translacional , Sequenciamento Completo do Exoma
20.
Cochrane Database Syst Rev ; 9: CD012192, 2019 09 17.
Artigo em Inglês | MEDLINE | ID: mdl-31529804

RESUMO

BACKGROUND: 'Infertility' is defined as the failure to achieve pregnancy after 12 months or more of regular unprotected sexual intercourse. One in six couples experience a delay in becoming pregnant. In vitro fertilisation (IVF) is one of the assisted reproductive techniques used to enable couples to achieve a live birth. One of the processes involved in IVF is embryo culture in an incubator, where a stable environment is created and maintained. The incubators are set at approximately 37°C, which is based on the human core body temperature, although several studies have shown that this temperature may in fact be lower in the female reproductive tract and that this could be beneficial. In this review we have included randomised controlled trials which compared different temperatures of embryo culture. OBJECTIVES: To assess different temperatures of embryo culture for human assisted reproduction, which may lead to higher live birth rates. SEARCH METHODS: We searched the following databases and trial registers: the Cochrane Gynaecology and Fertility (CGF) Group Specialised Register of Controlled Trials, the Cochrane Central Register of Studies Online, MEDLINE, Embase, PsycINFO, CINAHL, clinicaltrials.gov, The World Health Organization International Trials Registry Platform search portal, DARE, Web of Knowledge, OpenGrey, LILACS database, PubMed and Google Scholar. Furthermore, we manually searched the references of relevant articles and contacted experts in the field to obtain additional data. We did not restrict the search by language or publication status. We performed the last search on 6 March 2019. SELECTION CRITERIA: Two review authors independently screened the titles and abstracts of articles retrieved by the search. Full texts of potentially eligible randomised controlled trials (RCTs) were obtained and screened. We included all RCTs which compared different temperatures of embryo culture in IVF or intracytoplasmic sperm injection (ICSI), with a minimum difference in temperature between the two incubators of ≥ 0.5°C. The search process is shown in the PRISMA flow chart. DATA COLLECTION AND ANALYSIS: Two review authors independently assessed trial eligibility and risk of bias and extracted data from the included studies; the third review author resolved any disagreements. We contacted trial authors to provide additional data. The primary review outcomes were live birth and miscarriage. Clinical pregnancy, ongoing pregnancy, multiple pregnancy and adverse events were secondary outcomes. All extracted data were dichotomous outcomes, and odds ratios (OR) were calculated with 95% confidence intervals (CIs) on an intention-to-treat basis. We assessed the overall quality of the evidence for the main comparisons using GRADE methods. MAIN RESULTS: We included three RCTs, with a total of 563 women, that compared incubation of embryos at 37.0°C or 37.1°C with a lower incubator temperature (37.0°C versus 36.6°C, 37.1°C versus 36.0°C, 37.0° versus 36.5°C). Live birth, miscarriage, clinical pregnancy, ongoing pregnancy and multiple pregnancy were reported. After additional information from the authors, we confirmed one study as having no adverse events; the other two studies did not report adverse events. We did not perform a meta-analysis as there were not enough studies included per outcome. Live birth was not graded since there were no data of interest available. The evidence for the primary outcome, miscarriage, was of very low quality. The evidence for the secondary outcomes, clinical pregnancy, ongoing pregnancy and multiple pregnancy was also of very low quality. We downgraded the evidence because of high risk of bias (for performance bias) and imprecision due to limited included studies and wide CIs.Only one study reported the primary outcome, live birth (n = 52). They performed randomisation at the level of oocytes and not per woman, and used a paired design whereby two embryos, one from 36.0°C and one from 37.0°C, were transferred. The data from this study were not interpretable in a meaningful way and therefore not presented. Only one study reported miscarriage. We are uncertain whether incubation at a lower temperature decreases the miscarriage (odds ratio (OR) 0.90, 95% CI 0.52 to 1.55; 1 study, N = 412; very low-quality evidence).Of the two studies that reported clinical pregnancy, only one of them performed randomisation per woman. We are uncertain whether a lower temperature improves clinical pregnancy compared to 37°C for embryo incubation (OR 1.08, 95% CI 0.73 to 1.60; 1 study, N = 412; very low-quality evidence). For the outcome, ongoing pregnancy, we are uncertain if a lower temperature is better than 37°C (OR 1.10, 95% CI 0.75 to 1.62; 1 study, N = 412; very low quality-evidence). Multiple pregnancy was reported by two studies, one of which used a paired design, which made it impossible to report the data per temperature. We are uncertain if a temperature lower than 37°C reduces multiple pregnancy (OR 0.80, 95% CI 0.31 to 2.07; 1 study, N = 412; very low-quality evidence). There was insufficient evidence to make a conclusion regarding adverse events, as no studies reported data suitable for analysis. AUTHORS' CONCLUSIONS: This review evaluated different temperatures for embryo culture during IVF. There is a lack of evidence for the majority of outcomes in this review. Based on very low-quality evidence, we are uncertain if incubating at a lower temperature than 37°C improves pregnancy outcomes. More RCTs are needed for comparing different temperatures of embryo culture which require reporting of clinical outcomes as live birth, miscarriage, clinical pregnancy and adverse events.


Assuntos
Técnicas de Cultura Embrionária/métodos , Técnicas de Reprodução Assistida , Temperatura Ambiente , Feminino , Fertilização In Vitro , Humanos , Infertilidade , Nascimento Vivo , Gravidez , Resultado da Gravidez , Taxa de Gravidez , Gravidez Múltipla , Ensaios Clínicos Controlados Aleatórios como Assunto , Injeções de Esperma Intracitoplásmicas
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