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1.
Mar Environ Res ; 198: 106557, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38823094

RESUMO

Sexual reproduction of reef-building corals is vital for coral reef ecosystem recovery. Corals allocate limited energy to growth and reproduction, when being under environmental disturbance, which ultimately shapes the community population dynamics. In the present study, energetic and physiological parameters of both parental colonies and larvae of the coral Pocillopora damicornis were measured during their reproduction stage under four temperatures; 28 °C (low-temperature acclimation, LA), 29 °C (control temperature, CT), 31 °C (high-temperature acclimation, HA), and 32 °C (heat stress, HS). The results showed temperature changes altered the larvae release timing and fecundity in P. damicornis. Parental colonies exposed to the LA treatment exhibited reduced investment in reproduction and released fewer larvae, while retaining more energy for their development. However, each larva acquired higher energy and symbiont densities enabling survival through longer planktonic periods before settlement. In contrast, parental colonies exposed to the HA treatment had increased investment for reproduction and larvae output, while per larva gained less energy to mitigate the threat of higher temperature. Furthermore, the energy allocation processes restructured fatty acids concentration and composition in both parental colonies and larvae as indicated by shifts in membrane fluidity under adaptable temperature changes. Notably, parental colonies from the HS treatment expended more energy in response to heat stress, resulting in adverse effects, especially after larval release. Our study expands the current knowledge on the energy allocation strategies of P. damicornis and how it is impacted by temperature. Parental colonies employed different energy allocation strategies under distinct temperature regimes to optimize their development and offspring success, but under heat stress, both were compromised. Lipid metabolism is essential for the success of coral reproduction and further understanding their response to heat stress can improve intervention strategies for coral reef conservation in warmer future oceans.


Assuntos
Antozoários , Metabolismo Energético , Reprodução , Temperatura , Animais , Antozoários/fisiologia , Recifes de Corais , Larva/fisiologia , Larva/crescimento & desenvolvimento , Aclimatação/fisiologia
2.
J Morphol ; 285(6): e21742, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38837266

RESUMO

Chaetae are among the most extensively studied structures in polychaetes, serving as a defining morphological trait for annelids. Capitella teleta stands out as one of the few established annelid models for developmental and morphological studies, thus receiving significant scholarly attention. In this study, we unveil a previously unnoticed glandular structure associated with chaetae within the larvae of C. teleta. Our investigations demonstrate the absence of comparable structures in the chaetal follicles of adults and juveniles (older than 1 week), as well as during active chaetogenesis, underscoring the transient nature of these glands. This indicates that larval chaetal follicles transform into a gland that later disappears. Utilizing histology and transmission electron microscopy, we characterized these glands. Our findings underscore the diversity of chaetal ultrastructure in annelids and show that, even in well-studied species, novel morphological details can be found. We emphasize the importance of examining various life-history stages to capture such transient morphological features. This work lays a crucial morphological foundation and deepens our understanding of chaetae and chaetogenesis in C. teleta, paving the way for more accurate interpretations of future experimental studies on chaetogenesis in this species.


Assuntos
Larva , Poliquetos , Animais , Poliquetos/anatomia & histologia , Poliquetos/crescimento & desenvolvimento , Poliquetos/ultraestrutura , Larva/ultraestrutura , Larva/anatomia & histologia , Larva/crescimento & desenvolvimento , Microscopia Eletrônica de Transmissão , Anelídeos/anatomia & histologia , Anelídeos/ultraestrutura , Anelídeos/crescimento & desenvolvimento
3.
Microb Ecol ; 87(1): 81, 2024 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-38829379

RESUMO

Koinobiont endoparasitoids regulate the physiology of their hosts through altering host immuno-metabolic responses, processes which function in tandem to shape the composition of the microbiota of these hosts. Here, we employed 16S rRNA and ITS amplicon sequencing to investigate whether parasitization by the parasitoid wasps, Diachasmimorpha longicaudata (Ashmaed) (Hymenoptera: Braconidae) and Psyttalia cosyrae (Wilkinson) (Hymenoptera: Braconidae), induces gut dysbiosis and differentially alter the gut microbial (bacteria and fungi) communities of an important horticultural pest, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). We further investigated the composition of bacterial communities of adult D. longicaudata and P. cosyrae to ascertain whether the adult parasitoids and parasitized host larvae share microbial taxa through transmission. We demonstrated that parasitism by D. longicaudata induced significant gut perturbations, resulting in the colonization and increased relative abundance of pathogenic gut bacteria. Some pathogenic bacteria like Stenotrophomonas and Morganella were detected in both the guts of D. longicaudata-parasitized B. dorsalis larvae and adult D. longicaudata wasps, suggesting a horizontal transfer of microbes from the parasitoid to the host. The bacterial community of P. cosyrae adult wasps was dominated by Arsenophonus nasoniae, whereas that of D. longicaudata adults was dominated by Paucibater spp. and Pseudomonas spp. Parasitization by either parasitoid wasp was associated with an overall reduction in fungal diversity and evenness. These findings indicate that unlike P. cosyrae which is avirulent to B. dorsalis, parasitization by D. longicaudata induces shifts in the gut bacteriome of B. dorsalis larvae to a pathobiont-dominated community. This mechanism possibly enhances its virulence against the pest, further supporting its candidacy as an effective biocontrol agent of this frugivorous tephritid fruit fly pest.


Assuntos
Bactérias , Microbioma Gastrointestinal , Larva , RNA Ribossômico 16S , Tephritidae , Vespas , Animais , Tephritidae/microbiologia , Tephritidae/parasitologia , Vespas/microbiologia , Vespas/fisiologia , Bactérias/genética , Bactérias/classificação , Bactérias/isolamento & purificação , Larva/microbiologia , Larva/parasitologia , Larva/crescimento & desenvolvimento , RNA Ribossômico 16S/genética , Fungos/genética , Fungos/fisiologia , Interações Hospedeiro-Parasita , Microbiota , Disbiose/microbiologia , Disbiose/parasitologia
4.
BMC Genomics ; 25(1): 591, 2024 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-38867206

RESUMO

BACKGROUND: The Portuguese oyster Crassostrea angulata, a bivalve of significant economic and ecological importance, has faced a decline in both production and natural populations due to pathologies, climate change, and anthropogenic factors. To safeguard its genetic diversity and improve reproductive management, cryopreservation emerges as a valuable strategy. However, the cryopreservation methodologies lead to some damage in structures and functions of the cells and tissues that can affect post-thaw quality. Transcriptomics may help to understand the molecular consequences related to cryopreservation steps and therefore to identify different freezability biomarkers. This study investigates the molecular damage induced by cryopreservation in C. angulata D-larvae, focusing on two critical steps: exposure to cryoprotectant solution and the freezing/thawing process. RESULTS: Expression analysis revealed 3 differentially expressed genes between larvae exposed to cryoprotectant solution and fresh larvae and 611 differentially expressed genes in cryopreserved larvae against fresh larvae. The most significantly enriched gene ontology terms were "carbohydrate metabolic process", "integral component of membrane" and "chitin binding" for biological processes, cellular components and molecular functions, respectively. Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified the "neuroactive ligand receptor interaction", "endocytosis" and "spliceosome" as the most enriched pathways. RNA sequencing results were validate by quantitative RT-PCR, once both techniques presented the same gene expression tendency and a group of 11 genes were considered important molecular biomarkers to be used in further studies for the evaluation of cryodamage. CONCLUSIONS: The current work provided valuable insights into the molecular repercussions of cryopreservation on D-larvae of Crassostrea angulata, revealing that the freezing process had a more pronounced impact on larval quality compared to any potential cryoprotectant-induced toxicity. Additionally, was identify 11 genes serving as biomarkers of freezability for D-larvae quality assessment. This research contributes to the development of more effective cryopreservation protocols and detection methods for cryodamage in this species.


Assuntos
Crassostrea , Criopreservação , Crioprotetores , Perfilação da Expressão Gênica , Larva , Animais , Crassostrea/genética , Crassostrea/crescimento & desenvolvimento , Crioprotetores/farmacologia , Crioprotetores/toxicidade , Larva/genética , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Transcriptoma , Ontologia Genética
5.
Rev Bras Parasitol Vet ; 33(2): e019723, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38896756

RESUMO

Ctenocephalides felis felis is a relevant and widely distributed ectoparasite that acts as a vector of disease-causing pathogens. Moreover, it is responsible for economic losses due the use of harmful chemicals to the environment and that favor the emergence of insecticide-resistant populations. Research on entomopathogenic nematodes may open up an alternative route to the insect chemical control. The present study aimed to evaluate the killing efficacy of Heterorhabditis bacteriophora (HP88) against C. felis larvae in 400 µL, 600 µL and 1000 µL of suspension containing 120, 160 and 200 infective juveniles/larva and 600 µL of suspension containing the same concentrations of Heterorhabditis indica (LPP30), divided into two groups (absence and presence of diet) and a control group with three replications containing only distilled water. In the bioassay with H. bacteriophora, the groups in 600 µL of suspension showed higher mortality rates than those in the other tested volumes, which were above 80% at all concentrations. On the other hand, H. indica achieved mortality rates above 70% at all concentrations used. Results indicate that flea larvae are susceptible to in vitro infection by H. bacteriophora and H. indica.


Assuntos
Ctenocephalides , Larva , Animais , Larva/crescimento & desenvolvimento , Ctenocephalides/crescimento & desenvolvimento , Controle Biológico de Vetores , Nematoides
6.
Parasitol Res ; 123(6): 243, 2024 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-38874599

RESUMO

Diphyllobothriosis, a fish-borne zoonosis in South America, is mainly caused by the Pacific broad tapeworm Adenocephalus pacificus Nybelin, 1931, a parasite of considerable concern in fishery resources due to its impact on public health. A new diphyllobothrid, Diphyllobothrium sprakeri Hernández-Orts et al. Parasites Vectors 14:219, 2021, was recently described from sea lions from the Pacific Coast, but marine fish acting as intermediate hosts are unknown. The objective of this study was to confirm the presence of plerocercoid larvae of Diphyllobothriidae Lühe, 1910 (Cestoda: Diphyllobothriidea) in nine fish species of commercial importance in Peru. Of a total of 6999 fish (5861 Engraulis ringens, 853 Sciaena deliciosa, 6 Sciaena callaensis, 171 Scomber japonicus, 40 Trachurus murphyi, 40 Ariopsis seemanni, 18 Merluccius peruanus, 5 Sarda chiliensis, and 5 Coryphaena hippurus), 183 were infected with plerocercoid larvae, representing a total prevalence of 2.61% and a mean intensity of 3.2. Based on mtDNA cox1 sequences of 43 plerocercoids, a phylogenetic analysis revealed that 41 belong to A. pacificus and two to D. sprakeri. These findings are first molecular data for D. sprakeri larvae, and the infections of E. ringens and T. murphyi by plerocercoid larvae represent the first records of intermediate/paratenic hosts for this species. Hence, the findings of the current study enhance our understanding of the presence of diphyllobothriid species in commercial fish from the Southeastern Pacific Ocean and their potential impact on seafood safety for local human populations.


Assuntos
Doenças dos Peixes , Peixes , Larva , Animais , Peru/epidemiologia , Doenças dos Peixes/parasitologia , Doenças dos Peixes/epidemiologia , Peixes/parasitologia , Prevalência , Larva/classificação , Larva/crescimento & desenvolvimento , Larva/genética , Filogenia , Infecções por Cestoides/veterinária , Infecções por Cestoides/parasitologia , Infecções por Cestoides/epidemiologia , Cestoides/genética , Cestoides/classificação , Cestoides/isolamento & purificação , Diphyllobothrium/genética , Diphyllobothrium/classificação , Diphyllobothrium/isolamento & purificação , Difilobotríase/epidemiologia , Difilobotríase/parasitologia , Difilobotríase/veterinária , DNA de Helmintos/genética
7.
Development ; 151(12)2024 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-38885078

RESUMO

The steroid hormone ecdysone (Ec) is secreted from the prothoracic gland for growth in the developing Drosophila larva. How Ec-dependent regeneration can occur despite a drop in circulating Ec in the injured developing larvae remains unclear. In a new study in Development, Kenneth Moberg and colleagues find that injury induces local Ec synthesis at the wounded site to delay development and promote tissue repair in Drosophila. To learn more about the story behind the paper, we caught up with first author Douglas Terry and corresponding author Kenneth Moberg, Professor of Cell Biology at Emory University School of Medicine, USA.


Assuntos
Ecdisona , Animais , Ecdisona/metabolismo , Drosophila , História do Século XXI , História do Século XX , Biologia do Desenvolvimento/história , Humanos , Larva/crescimento & desenvolvimento , Drosophila melanogaster
8.
PLoS Biol ; 22(6): e3002662, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38870210

RESUMO

The polygonal shape of cells in proliferating epithelia is a result of the tensile forces of the cytoskeletal cortex and packing geometry set by the cell cycle. In the larval Drosophila epidermis, two cell populations, histoblasts and larval epithelial cells, compete for space as they grow on a limited body surface. They do so in the absence of cell divisions. We report a striking morphological transition of histoblasts during larval development, where they change from a tensed network configuration with straight cell outlines at the level of adherens junctions to a highly folded morphology. The apical surface of histoblasts shrinks while their growing adherens junctions fold, forming deep lobules. Volume increase of growing histoblasts is accommodated basally, compensating for the shrinking apical area. The folded geometry of apical junctions resembles elastic buckling, and we show that the imbalance between the shrinkage of the apical domain of histoblasts and the continuous growth of junctions triggers buckling. Our model is supported by laser dissections and optical tweezer experiments together with computer simulations. Our analysis pinpoints the ability of histoblasts to store mechanical energy to a much greater extent than most other epithelial cell types investigated so far, while retaining the ability to dissipate stress on the hours time scale. Finally, we propose a possible mechanism for size regulation of histoblast apical size through the lateral pressure of the epidermis, driven by the growth of cells on a limited surface. Buckling effectively compacts histoblasts at their apical plane and may serve to avoid physical harm to these adult epidermis precursors during larval life. Our work indicates that in growing nondividing cells, compressive forces, instead of tension, may drive cell morphology.


Assuntos
Epiderme , Larva , Morfogênese , Animais , Epiderme/metabolismo , Larva/crescimento & desenvolvimento , Drosophila melanogaster/crescimento & desenvolvimento , Células Epidérmicas , Células Epiteliais/citologia , Células Epiteliais/fisiologia , Células Epiteliais/metabolismo , Fenômenos Biomecânicos , Junções Aderentes/metabolismo , Forma Celular , Simulação por Computador , Drosophila/crescimento & desenvolvimento , Modelos Biológicos
9.
Sci Rep ; 14(1): 13721, 2024 06 14.
Artigo em Inglês | MEDLINE | ID: mdl-38877078

RESUMO

The beet armyworm, Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae), has become a significant pest of chickpea in recent years. The polyphagous nature allows it to survive on various hosts during the off-season, creating a great menace to the crop in the following season. To assess the incidence and document the alternate hosts of S. exigua, a rapid roving survey was conducted in 11 chickpea-growing areas of Prakasam district, Andhra Pradesh, India. Additionally, the life history traits of S. exigua were studied on major alternate host plants under laboratory conditions (27 ± 1 °C and 70 ± 2% RH) to understand the survival, life expectancy and potential contribution to future populations. The results show that, among the different crops surveyed, the maximum larval incidence was noticed in maize (1.93 larvae/plant), cowpea (1.73 larvae/plant), and sunflower (1.68 larvae/plant) during the off-season. Life history studies of S. exigua showed that highest larval survival percentage was observed on chickpea (83.6%), while the lowest was on maize (44.5%). The mean developmental time for larvae was longest on maize (27.1 days) and shortest on chickpea (14.9 days). Larvae did not develop beyond the third instar when fed with chilli. The growth index statistics showed chickpea (9.2) was the most suitable host plant, whereas maize (0.9) was the least suitable host. The age-stage-specific survival rate (Sxj) varied across developmental stages, and the survival curves overlapped, indicating different growth rates among individuals. The life expectancy (exj) at age zero was highest on groundnut (37.06 days). The intrinsic rate of increase (r) of S. exigua was lowest on maize (0.10 ± 0.0013) and highest on chickpea (0.22 ± 0.0010). Similarly, the net reproductive rate (R0) was highest on chickpea (846.39 ± 18.22) and lowest on maize (59.50 ± 2.06). The population doubled every 3.08 ± 0.011 days on chickpea compared to 7.22 ± 0.80 days on maize. The study conclusively indicates that chickpea and sunflower, primarily cultivated during the rabi season in India, are the most preferred hosts for S. exigua. In contrast, maize and cotton, mainly grown during the kharif season, are less preferred and merely support the pest's survival. Consequently, S. exigua switches hosts between different crops growing seasons, so effective management of S. exigua during the kharif season can help prevent pest outbreaks during the rabi season.


Assuntos
Cicer , Larva , Estações do Ano , Spodoptera , Animais , Spodoptera/crescimento & desenvolvimento , Spodoptera/fisiologia , Larva/crescimento & desenvolvimento , Cicer/parasitologia , Produtos Agrícolas/parasitologia , Índia , Zea mays/parasitologia , Vigna/parasitologia , Vigna/crescimento & desenvolvimento
10.
Ticks Tick Borne Dis ; 15(5): 102363, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38852540

RESUMO

Among cattle, Bos taurus breeds and their crosses are more sensitive to tick infestations than Bos indicus breeds that are more resistant to infestation and more adaptable to tropical climates. The presence of susceptible individuals in herds and inadequate tick control lead to direct and indirect losses in the meat production chain, in addition to increased mortality due to cattle tick fever. The objective of this study was to describe, compare and rank the sensitivity of different breeds of stabled cattle to the tick Rhipicephalus microplus and to present, as an innovative result, a scale called the Tick Ruler. Secondary data on the number of retrieved engorged females, engorged female ticks' weight, egg mass weight and number of larvae were extracted from research reports of experiments conducted over 18 years with eight breeds to describe and report the sensitivity of the breeds to artificial infestation by R. microplus larvae. For analyses, the recovery rate of engorged female ticks and the percentile of dispersion of individuals in their respective races were calculated, and comparison of these percentiles between races was performed. The ranking of the percentiles resulted in the organization of the breeds by their susceptibility to R. microplus; we call this scale the "Tick Ruler." The ruler is a simple, easy-to-understand tool that can be used by technicians and producers to evaluate the tick sensitivity of a breed of interest and can assist producers in decision-making to find a balance between increased production gains and the risk of economic losses depending on the breed composition in a cattle herd.


Assuntos
Doenças dos Bovinos , Larva , Rhipicephalus , Infestações por Carrapato , Animais , Rhipicephalus/fisiologia , Rhipicephalus/genética , Bovinos , Infestações por Carrapato/veterinária , Infestações por Carrapato/parasitologia , Feminino , Doenças dos Bovinos/parasitologia , Larva/crescimento & desenvolvimento , Larva/fisiologia , Cruzamento
11.
J Agric Food Chem ; 72(23): 12935-12945, 2024 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-38822796

RESUMO

Blister beetles of Epicauta impressicornis have attracted attention because they contain a large amount of cantharidin (CTD). To date, however, the synthesis and transfer of CTD in adults of E. impressicornis are largely unknown. Here, we showed that the larvae E. impressicornis are capable of synthesizing CTD and they consume CTD during pupation. Before sexual maturity, both male and female adults synthesized a small amount of CTD, while after sexual maturity, males produced larger amounts of CTD, but females did not. The newly synthesized CTD in males first appeared in the hemolymph and then accumulated in the reproductive system. During the mating, the males transferred CTD to the reproductive system of females. In addition, a farnesyl pyrophosphate synthase (FPPS) gene was identified in male E. impressicornis. RNA-seq analysis, quantitative RT-PCR, and RNA interference analyses were conducted to investigate expression patterns and the functional roles of E. impressicornis FPPS (EiFPPS). Our results indicate that EiFPPS is highly expressed in the fat body of males. Moreover, the knock-down of EiFPPS led to a significant decrease in CTD synthesis. The current study indicates that EiFPPS is expressed in the fat body to regulate CTD synthesis in male E. impressicornis blister beetles.


Assuntos
Cantaridina , Besouros , Corpo Adiposo , Geraniltranstransferase , Proteínas de Insetos , Animais , Besouros/genética , Besouros/metabolismo , Besouros/enzimologia , Cantaridina/metabolismo , Masculino , Corpo Adiposo/metabolismo , Corpo Adiposo/enzimologia , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Geraniltranstransferase/genética , Geraniltranstransferase/metabolismo , Feminino , Larva/crescimento & desenvolvimento , Larva/genética , Larva/metabolismo
12.
Aquat Toxicol ; 272: 106979, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38823072

RESUMO

Tris(2-chloroethyl) phosphate (TCEP) and tris(1­chloro-2-propyl) phosphate (TCPP) are widely used as chlorinated organophosphate flame retardants (OPFRs) due to their fire-resistance capabilities. However, their extensive use has led to their permeation and pollution in aquatic environments. Using amphibians, which are non-model organisms, to test the toxic effects of OPFRs is relatively uncommon. This study examined the acute and chronic toxicity differences between TCEP and TCPP on Polypedates megacephalus tadpoles and evaluated the potential ecological risks to tadpoles in different aquatic environments using the risk quotient (RQ). In acute toxicity assay, the tadpole survival rates decreased with increased exposure time and concentrations, with TCEP exhibiting higher LC50 values than TCPP, at 305.5 mg/L and 70 mg/L, respectively. In the chronic assay, prolonged exposure to 300 µg/L of both substances resulted in similar adverse effects on tadpole growth, metamorphosis, and hepatic antioxidant function. Based on RQ values, most aquatic environments did not pose an ecological risk to tadpoles. However, the analysis showed that wastewater presented higher risks than rivers and drinking water, and TCPP posed a higher potential risk than TCEP in all examined aquatic environments. These findings provide empirical evidence to comprehend the toxicological effects of OPFRs on aquatic organisms and to assess the safety of aquatic environments.


Assuntos
Anuros , Retardadores de Chama , Larva , Organofosfatos , Compostos Organofosforados , Poluentes Químicos da Água , Animais , Retardadores de Chama/toxicidade , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Poluentes Químicos da Água/toxicidade , Compostos Organofosforados/toxicidade , Medição de Risco , Organofosfatos/toxicidade , Anuros/crescimento & desenvolvimento , Metamorfose Biológica/efeitos dos fármacos , Testes de Toxicidade Aguda , Dose Letal Mediana
13.
BMC Genomics ; 25(1): 585, 2024 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-38862878

RESUMO

BACKGROUND: Anguillid eels spend their larval period as leptocephalus larvae that have a unique and specialized body form with leaf-like and transparent features, and they undergo drastic metamorphosis to juvenile glass eels. Less is known about the transition of leptocephali to the glass eel stage, because it is difficult to catch the metamorphosing larvae in the open ocean. However, recent advances in rearing techniques for the Japanese eel have made it possible to study the larval metamorphosis of anguillid eels. In the present study, we investigated the dynamics of gene expression during the metamorphosis of Japanese eel leptocephali using RNA sequencing. RESULTS: During metamorphosis, Japanese eels were classified into 7 developmental stages according to their morphological characteristics, and RNA sequencing was used to collect gene expression data from each stage. A total of 354.8 million clean reads were generated from the body and 365.5 million from the head, after the processing of raw reads. For filtering of genes that characterize developmental stages, a classification model created by a Random Forest algorithm was built. Using the importance of explanatory variables feature obtained from the created model, we identified 46 genes selected in the body and 169 genes selected in the head that were defined as the "most characteristic genes" during eel metamorphosis. Next, network analysis and subsequently gene clustering were conducted using the most characteristic genes and their correlated genes, and then 6 clusters in the body and 5 clusters in the head were constructed. Then, the characteristics of the clusters were revealed by Gene Ontology (GO) enrichment analysis. The expression patterns and GO terms of each stage were consistent with previous observations and experiments during the larval metamorphosis of the Japanese eel. CONCLUSION: Genome and transcriptome resources have been generated for metamorphosing Japanese eels. Genes that characterized metamorphosis of the Japanese eel were identified through statistical modeling by a Random Forest algorithm. The functions of these genes were consistent with previous observations and experiments during the metamorphosis of anguillid eels.


Assuntos
Anguilla , Perfilação da Expressão Gênica , Larva , Metamorfose Biológica , Animais , Metamorfose Biológica/genética , Larva/crescimento & desenvolvimento , Larva/genética , Anguilla/genética , Anguilla/crescimento & desenvolvimento , Transcriptoma , Regulação da Expressão Gênica no Desenvolvimento
14.
Elife ; 122024 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-38842917

RESUMO

The atypical cadherins Fat and Dachsous (Ds) signal through the Hippo pathway to regulate growth of numerous organs, including the Drosophila wing. Here, we find that Ds-Fat signaling tunes a unique feature of cell proliferation found to control the rate of wing growth during the third instar larval phase. The duration of the cell cycle increases in direct proportion to the size of the wing, leading to linear-like growth during the third instar. Ds-Fat signaling enhances the rate at which the cell cycle lengthens with wing size, thus diminishing the rate of wing growth. We show that this results in a complex but stereotyped relative scaling of wing growth with body growth in Drosophila. Finally, we examine the dynamics of Fat and Ds protein distribution in the wing, observing graded distributions that change during growth. However, the significance of these dynamics is unclear since perturbations in expression have negligible impact on wing growth.


Assuntos
Caderinas , Ciclo Celular , Proteínas de Drosophila , Drosophila melanogaster , Transdução de Sinais , Asas de Animais , Animais , Asas de Animais/crescimento & desenvolvimento , Asas de Animais/metabolismo , Proteínas de Drosophila/metabolismo , Proteínas de Drosophila/genética , Drosophila melanogaster/crescimento & desenvolvimento , Drosophila melanogaster/metabolismo , Caderinas/metabolismo , Larva/crescimento & desenvolvimento , Larva/metabolismo , Proliferação de Células , Moléculas de Adesão Celular
15.
Elife ; 122024 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-38904987

RESUMO

Numerous roles for the Alk receptor tyrosine kinase have been described in Drosophila, including functions in the central nervous system (CNS), however the molecular details are poorly understood. To gain mechanistic insight, we employed Targeted DamID (TaDa) transcriptional profiling to identify targets of Alk signaling in the larval CNS. TaDa was employed in larval CNS tissues, while genetically manipulating Alk signaling output. The resulting TaDa data were analyzed together with larval CNS scRNA-seq datasets performed under similar conditions, identifying a role for Alk in the transcriptional regulation of neuroendocrine gene expression. Further integration with bulk and scRNA-seq datasets from larval brains in which Alk signaling was manipulated identified a previously uncharacterized Drosophila neuropeptide precursor encoded by CG4577 as an Alk signaling transcriptional target. CG4577, which we named Sparkly (Spar), is expressed in a subset of Alk-positive neuroendocrine cells in the developing larval CNS, including circadian clock neurons. In agreement with our TaDa analysis, overexpression of the Drosophila Alk ligand Jeb resulted in increased levels of Spar protein in the larval CNS. We show that Spar protein is expressed in circadian (clock) neurons, and flies lacking Spar exhibit defects in sleep and circadian activity control. In summary, we report a novel activity regulating neuropeptide precursor gene that is regulated by Alk signaling in the Drosophila CNS.


Assuntos
Quinase do Linfoma Anaplásico , Sistema Nervoso Central , Proteínas de Drosophila , Animais , Sistema Nervoso Central/metabolismo , Proteínas de Drosophila/metabolismo , Proteínas de Drosophila/genética , Quinase do Linfoma Anaplásico/metabolismo , Quinase do Linfoma Anaplásico/genética , Larva/metabolismo , Larva/genética , Larva/crescimento & desenvolvimento , Neuropeptídeos/metabolismo , Neuropeptídeos/genética , Transdução de Sinais , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Receptores Proteína Tirosina Quinases/metabolismo , Receptores Proteína Tirosina Quinases/genética , Drosophila/genética , Drosophila/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica
16.
Int J Mol Sci ; 25(11)2024 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-38891931

RESUMO

Serine peptidases (SPs) of the chymotrypsin S1A subfamily are an extensive group of enzymes found in all animal organisms, including insects. Here, we provide analysis of SPs in the yellow mealworm Tenebrio molitor transcriptomes and genomes datasets and profile their expression patterns at various stages of ontogeny. A total of 269 SPs were identified, including 137 with conserved catalytic triad residues, while 125 others lacking conservation were proposed as non-active serine peptidase homologs (SPHs). Seven deduced sequences exhibit a complex domain organization with two or three peptidase units (domains), predicted both as active or non-active. The largest group of 84 SPs and 102 SPHs had no regulatory domains in the propeptide, and the majority of them were expressed only in the feeding life stages, larvae and adults, presumably playing an important role in digestion. The remaining 53 SPs and 23 SPHs had different regulatory domains, showed constitutive or upregulated expression at eggs or/and pupae stages, participating in regulation of various physiological processes. The majority of polypeptidases were mainly expressed at the pupal and adult stages. The data obtained expand our knowledge on SPs/SPHs and provide the basis for further studies of the functions of proteins from the S1A subfamily in T. molitor.


Assuntos
Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Insetos , Tenebrio , Transcriptoma , Animais , Tenebrio/genética , Tenebrio/enzimologia , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Filogenia , Serina Proteases/genética , Serina Proteases/metabolismo , Larva/genética , Larva/crescimento & desenvolvimento , Sequência de Aminoácidos
17.
NPJ Biofilms Microbiomes ; 10(1): 51, 2024 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-38902226

RESUMO

Bacteria induced metamorphosis observed in nearly all marine invertebrates. However, the mechanism of bacteria regulating the larvae-juvenile metamorphosis remains unknown. Here, we test the hypothesis that c-di-GMP, a ubiquitous bacterial second-messenger molecule, directly triggers the mollusc Mytilus coruscus larval metamorphosis via the stimulator of interferon genes (STING) receptor. We determined that the deletion of c-di-GMP synthesis genes resulted in reduced c-di-GMP levels and biofilm-inducing activity on larval metamorphosis, accompanied by alterations in extracellular polymeric substances. Additionally, c-di-GMP extracted from tested varying marine bacteria all exhibited inducing activity on larval metamorphosis. Simultaneously, through pharmacological and molecular experiments, we demonstrated that M. coruscus STING (McSTING) participates in larval metamorphosis by binding with c-di-GMP. Our findings reveal that new role of bacterial c-di-GMP that triggers mussel larval metamorphosis transition, and extend knowledge in the interaction of bacteria and host development in marine ecosystems.


Assuntos
Biofilmes , GMP Cíclico , Larva , Metamorfose Biológica , Mytilus , Animais , Larva/microbiologia , Larva/crescimento & desenvolvimento , GMP Cíclico/análogos & derivados , GMP Cíclico/metabolismo , Biofilmes/crescimento & desenvolvimento , Mytilus/microbiologia , Mytilus/crescimento & desenvolvimento , Bactérias/genética , Bactérias/classificação , Bactérias/metabolismo , Bactérias/crescimento & desenvolvimento , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo
18.
BMC Genomics ; 25(1): 616, 2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38890587

RESUMO

The Drosophila eye has been an important model to understand principles of differentiation, proliferation, apoptosis and tissue morphogenesis. However, a single cell RNA sequence resource that captures gene expression dynamics from the initiation of differentiation to the specification of different cell types in the larval eye disc is lacking. Here, we report transcriptomic data from 13,000 cells that cover six developmental stages of the larval eye. Our data show cell clusters that correspond to all major cell types present in the eye disc ranging from the initiation of the morphogenetic furrow to the differentiation of each photoreceptor cell type as well as early cone cells. We identify dozens of cell type-specific genes whose function in different aspects of eye development have not been reported. These single cell data will greatly aid research groups studying different aspects of early eye development and will facilitate a deeper understanding of the larval eye as a model system.


Assuntos
Olho , Larva , Análise de Célula Única , Animais , Larva/genética , Larva/crescimento & desenvolvimento , Larva/metabolismo , Olho/metabolismo , Olho/crescimento & desenvolvimento , Perfilação da Expressão Gênica , Transcriptoma , Regulação da Expressão Gênica no Desenvolvimento , Drosophila/genética , Drosophila/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/crescimento & desenvolvimento , Análise de Sequência de RNA
19.
Animal ; 18(6): 101197, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38850579

RESUMO

To address multiple issues impacting the climate imbalance, insects, and in particular Tenebrio molitor, represent now a promising alternative for producing high-quality protein products with low environmental impact. As with any new species farmed on an industrial scale, insect breeding production must be improved through the accumulation of knowledge on rearing techniques and genetic management. Little information on the inheritance of agronomically interesting traits, dedicated to Tenebrio molitor, is available. This study aims to decipher the genetic parameters (heritability and genetic correlations) of reproduction, larval growth and survival, pupation rate and developmental time from a reference population made up of 1 931 sib-groups reared under pedigree, in controlled and stable environments and generated with single pair mating. Considering all sib-groups, 29 599 offspring have been generated and phenotyped over four generations to support this study and provide enough data to estimate, under linear animal models, the additive genetic and common environmental effects. Phenotypic analyses underlined an important variability among sib-groups and individuals, as for the total oviposition during 4 weeks counting (0-680 eggs, min - max, respectively) or larval body mass 63 days posteclosion (36.3-206.8 mg, min - max, respectively). Moderate to important heritability values have been obtained and ranged from 0.17 to 0.54 for reproduction phenotypes, 0.10-0.44 for growth parameters, 0.06-0.22 for developmental time and 0.10-0.17 for larval survival rates. The proportion of phenotypic variance explained by the environmental part varyies from 0.10 to 0.36 for reproductive traits, from 0.17 to 0.38 for growth parameters, from 0.06 to 0.36 for developmental time and 0.17-0.22 for survival rates. Genetic correlations underline relationships among phenotypes such as the trade-off between developmental time from egg to pupae and pupae weight (r2 = 0.48 ± 0.06). These important phenotypic variations coupled with promising heritability values pave the road for future breeding programs in Tenebrio molitor.


Assuntos
Cruzamento , Larva , Fenótipo , Reprodução , Tenebrio , Animais , Tenebrio/genética , Feminino , Masculino , Larva/crescimento & desenvolvimento , Larva/genética , Reprodução/genética , Oviposição/genética
20.
J Am Mosq Control Assoc ; 40(2): 109-111, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38811012

RESUMO

Updating the mosquito fauna occurring in a specific area is crucial, given that certain species serve as vectors capable of transmitting zoonotic arboviruses. This scientific note presents the first records of mosquitoes of the tribe Orthopodomyiini in the Yucatan Peninsula. Immature mosquitoes were collected on 2 occasions inside a large tree hole in Felipe Carrillo Puerto, Quintana Roo, Mexico. Thirteen adult specimens, reared from the immatures, were obtained and identified as Orthopodomyia kummi based on external characteristics of females and males. This species has been recorded in Panama, Costa Rica, El Salvador, Guatemala, Mexico, and marginally in the United States, but its presence in the Yucatan Peninsula had gone unnoticed until now. The knowledge about mosquitoes of the genus Orthopodomyia is limited, and their epidemiological importance remains uncertain. Therefore, further studies could provide insights into the ecological and infection dynamics associated with this species.


Assuntos
Distribuição Animal , Culicidae , Animais , México , Feminino , Masculino , Larva/crescimento & desenvolvimento
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