Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 5.447
Filtrar
1.
Anal Methods ; 16(28): 4691-4699, 2024 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-38973362

RESUMO

Herein, a new dual-model photoelectrochemical (PEC)/electrochemical (EC) sensor based on Z-scheme titanium dioxide (TiO2) disk/methylene blue (MB) sensibilization for the detection of kanamycin (Kana) was developed. Metal-organic framework-derived porous TiO2 disks were synthesized and exhibited excellent anodic photocurrent under visible light excitation. Subsequently, amino-labeled double-stranded DNA (dsDNA) was introduced into the modified electrode. Photocurrent was enhanced with MB embedded in dsDNA to form Z-scheme TiO2/MB sensibilization. When the target, Kana, was present, it specifically bound to the aptamer in the dsDNA, leading to the disruption of the dsDNA structure and the release of MB. This release of MB and the increase in target spatial resistance resulted in a significant weakening of PEC signal and a decreased oxidation peak current of MB. The PEC sensor successfully detected Kana in the range of 2-1000 pM with an LOD of 0.17 pM. Meanwhile, the EC sensor for Kana detection showed a linear range of 5-500 pM with an LOD of 1.8 pM. Additionally, the sensor exhibited excellent selectivity, reproducibility, stability, and good recoveries when applied to milk and honey samples. As a result, this method has the potential for application in ensuring food safety through the rapid determination of antibiotics in food.


Assuntos
Técnicas Eletroquímicas , Canamicina , Azul de Metileno , Leite , Titânio , Titânio/química , Canamicina/análise , Canamicina/química , Azul de Metileno/química , Técnicas Eletroquímicas/métodos , Técnicas Eletroquímicas/instrumentação , Leite/química , Animais , Limite de Detecção , Técnicas Biossensoriais/métodos , Mel/análise , Antibacterianos/análise , Antibacterianos/química , Processos Fotoquímicos , Reprodutibilidade dos Testes , Eletrodos
2.
Molecules ; 29(13)2024 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-38999146

RESUMO

This study aimed to develop an analytical method using HS-SPME/GC-MS to determine the volatile organic compound (VOC) profiles and evaluate the sensory attributes of cocoa honey from four cocoa varieties (CCN51, PS1319, SJ02, and Parazinho). Using a multivariate factorial experimental design, the HS-SPME/GC-MS method was optimized to determine the VOC profiles. Twenty previously trained tasters participated in the ranking descriptive analysis, while 108 consumers participated in the acceptance and purchase intention tests. A total of 84 volatile organic compounds were identified from various chemical classes, including acids, alcohols, aldehydes, esters, ketones, monoterpenes, oxygenated monoterpenoids, sesquiterpenes, and oxygenated sesquiterpenoids. Palmitic acid was the compound found in the highest concentration in all varieties (5.13-13.10%). Multivariate analysis tools identified key compounds for differentiation and grouping of the samples. The results revealed that the variety significantly influenced both the VOCs' concentrations and sensory profiles. The CCN51, PS1319, and SJ02 varieties exhibited the highest diversity of VOCs and sensory attributes. Notably, the SJ02 and CCN51 varieties demonstrated superior acceptability and purchase intention, with means ranging from 7.21 and 7.08 to 3.71 and 3.56, respectively. These results indicate their potential as promising sources of cocoa honey for the food industry.


Assuntos
Cacau , Cromatografia Gasosa-Espectrometria de Massas , Mel , Microextração em Fase Sólida , Compostos Orgânicos Voláteis , Compostos Orgânicos Voláteis/análise , Cacau/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Mel/análise , Microextração em Fase Sólida/métodos , Humanos , Adulto , Feminino , Masculino
3.
J Ethnobiol Ethnomed ; 20(1): 67, 2024 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-39020367

RESUMO

BACKGROUND: In recent decades, agricultural landscapes have been profoundly modified due to the intensification of agriculture, therefore leading to significant disturbances in all components of biodiversity. A survey on the knowledge of beekeeping realities and the use of phytosanitary products in areas of high honey production in Burkina Faso was carried out. Beekeeping realities design the state of beekeeping activities in the study localities. METHODS: The objective of this survey was to characterize Beekeeping operations and to assess the level of knowledge of beekeepers on the effects of the use of phytosanitary products through different beekeeping and agricultural practices. In this sense, 113 farmer beekeepers from the Boucle du Mouhoun, Hauts-Bassins and Nord regions in Burkina Faso were surveyed about their different beekeeping practices. RESULTS: The results obtained indicated that beekeeping is a secondary activity (96.47%) and is mainly practiced by men (90.27%). The respondents have mostly an average of 22 traditional hives. The majority of beekeepers have not received training (84.07%) on the hazards of plant protection products on their beekeeping farms. However, a large amount of beekeepers (70.73%) acknowledged that the use of plant protection products could be harmful to their activity. Hives are usually installed in or near the fields. The plant protection products used for crop protection are herbicides (27%), insecticides (23%), fungicides (8%), but especially mixed (42%). CONCLUSION: The results show that beekeeping in Burkina Faso remains traditional and is practiced for sociocultural reasons. The use of pesticides close to beekeeping could play a role in bee colony collapse taking place in these regions. Training beekeepers on the dangers of the chemicals they use in fields near hives is therefore essential.


Assuntos
Criação de Abelhas , Mel , Burkina Faso , Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Adulto , Abelhas , Inquéritos e Questionários , Fazendeiros , Animais , Conhecimento
4.
Sci Rep ; 14(1): 16594, 2024 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-39026016

RESUMO

For the detection of food adulteration, sensitive and reproducible analytical methods are required. Liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) is a highly sensitive method that can be used to obtain analytical fingerprints consisting of a variety of different components. Since the comparability of measurements carried out with different devices and at different times is not given, specific adulterants are usually detected in targeted analyses instead of analyzing the entire fingerprint. However, this comprehensive analysis is desirable in order to stay ahead in the race against food fraudsters, who are constantly adapting their adulterations to the latest state of the art in analytics. We have developed and optimized an approach that enables the separate processing of untargeted LC­HRMS data obtained from different devices and at different times. We demonstrate this by the successful determination of the geographical origin of honey samples using a random forest model. We then show that this approach can be applied to develop a continuously learning classification model and our final model, based on data from 835 samples, achieves a classification accuracy of 94% for 126 test samples from 6 different countries.


Assuntos
Análise de Alimentos , Aprendizado de Máquina , Espectrometria de Massas , Cromatografia Líquida/métodos , Espectrometria de Massas/métodos , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Mel/análise , Espectrometria de Massa com Cromatografia Líquida
5.
Braz J Biol ; 84: e281066, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38922195

RESUMO

The identification of meliponicultural flora is fundamental for the preservation of bees, as well as enabling the development of pollinator management and reforestation programs. In this context, this study aimed to investigate the types of pollen found in the honey and pollen pots of the Melipona fasciculata bee in Arari, State of Maranhão, between August 2022 and July 2023. 40 types of pollen were identified, distributed among 18 botanical families, with Fabaceae standing out (46.15% in pollen pots and 37.86% in honey pots), Myrtaceae (11.53% in pollen and 13.51% in honey) and Rubiaceae (7.69% in pollen and 5.40% in honey). Sapindaceae, in the honey pots, accounted for 5.40%. The other families showed lower percentages, 3.84% in the pollen pots and 2.70% in the honey pots. In terms of similarity, two distinct groups were observed in both the pollen and honey pots. In the pollen pots, group A (May-June ~ 97%) and group B (Feb-Mar ~ 99%) stood out, while in the honey pots, group A (Mar-Apr ~ 98%) and group B (Jun-Jul-Sept ~ 98%) showed the highest percentages. These findings highlight the wide range of resources used by the M. fasciculata species, as well asits preference for Fabaceae and Myrtaceae, due to the diversity and availability of trophic resources. An understanding of the meliponicultural flora is essential to support effective conservation strategies, which aim not only to guarantee the survival of the bees, but also to ensure the continued production of honey, a resource of great importance to local communities.


Assuntos
Mel , Pólen , Pólen/classificação , Mel/análise , Abelhas/classificação , Abelhas/fisiologia , Animais , Brasil , Estações do Ano , Polinização
6.
Int J Mol Sci ; 25(12)2024 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-38928296

RESUMO

Honey is traditionally used for its medicinal properties attributed to its antibacterial and antioxidant effects. It is considered a natural alternative to conventional antibiotics. This effect has been attributed to their physico-chemical properties, as various chemical parameters can synergistically influence this effect. The aim of this study is to assess Spanish honeys of diverse botanical origins for their antibacterial efficacy against Staphylococcus epidermidis, correlating their physico-chemical attributes, (poly)phenol content, and antioxidant activity. The methods included colour determination via two methodologies, acidity, pH, moisture content, and sugar concentration. (Poly)phenol content was quantified using the Folin-Ciocalteau method, while antioxidant activity was evaluated via the FRAP method. Subsequently, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against S. epidermidis were investigated with different concentrations of honeys. The results revealed a direct relationship between honey darkness, (poly)phenol concentration, antioxidant activity, and antibacterial efficacy. Darker honeys exhibited higher (poly)phenol levels, greater antioxidant activity, and consequently, lower MIC and MBC values, showing enhanced antibacterial properties. These findings underscore the potential of honey as a therapeutic agent against S. epidermidis, particularly in wound healing applications to avoid infection. Further research into honey's multifaceted properties is warranted to unveil novel therapeutic avenues in healthcare.


Assuntos
Antibacterianos , Antioxidantes , Mel , Testes de Sensibilidade Microbiana , Staphylococcus epidermidis , Staphylococcus epidermidis/efeitos dos fármacos , Mel/análise , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Espanha
7.
Med Mycol ; 62(7)2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38936838

RESUMO

Candida auris is an emerging fungal pathogen responsible for healthcare-associated infections and outbreaks with high mortality around the world. It readily colonizes the skin, nares, respiratory and urinary tract of hospitalized patients, and such colonization may lead to invasive Candida infection in susceptible patients. However, there is no recommended decolonization protocol for C. auris by international health authorities. The aim of this study is to evaluate the susceptibility of C. auris to commonly used synthetic and natural antiseptic products using an in vitro, broth microdilution assay. Synthetic antiseptics including chlorhexidine, povidone-iodine, and nystatin were shown to be fungicidal against C. auris. Among the natural antiseptics tested, tea tree oil and manuka oil were both fungicidal against C. auris at concentrations less than or equal to 1.25% (v/v). Manuka honey inhibited C. auris at 25% (v/v) concentrations. Among the commercial products tested, manuka body wash and mouthwash were fungicidal against C. auris at concentrations less than or equal to 0.39% (w/v) and 6.25% (v/v) of products as supplied for use, respectively, while tea tree body wash and MedihoneyTM wound gel demonstrated fungistatic properties. In conclusion, this study demonstrated good in vitro antifungal efficacy of tea tree oil, manuka oil, manuka honey, and commercially available antiseptic products containing these active ingredients. Future studies are warranted to evaluate the effectiveness of these antiseptic products in clinical settings.


Candida auris is an emerging superbug fungus that poses a serious threat to global public health. The excellent antifungal efficacy of natural antiseptics and their commercial hygiene products provide new insights into the development of an alternative decolonization regimen against C. auris.


Assuntos
Anti-Infecciosos Locais , Antifúngicos , Candida auris , Testes de Sensibilidade Microbiana , Anti-Infecciosos Locais/farmacologia , Antifúngicos/farmacologia , Humanos , Candida auris/efeitos dos fármacos , Óleo de Melaleuca/farmacologia , Mel , Clorexidina/farmacologia , Leptospermum/química
8.
Int J Food Microbiol ; 421: 110789, 2024 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-38879955

RESUMO

The Protected Designation of Origin (PDO) indication for foods intends to guarantee the conditions of production and the geographical origin of regional products within the European Union. Honey products are widely consumed due to their health-promoting properties and there is a general interest in tracing their authenticity. In this regard, metagenomics sequencing and machine learning (ML) have been proposed as complementary technologies to improve the traceability methods of foods. Therefore, the aim of this study was to analyze the metagenomic profiles of Spanish honeys from three different PDOs (Granada, Tenerife and Villuercas-Ibores), and compare them with non-PDO honeys using ML models (PLS, RF, LOGITBOOST, and NNET). According to the results obtained, non-PDO honeys and Granada PDO showed higher beta diversity values than Tenerife and Villuercas-Ibores PDOs. ML classification of honey products allowed the identification of different microbial biomarkers of the geographical origin of honeys: Lactobacillus kunkeei, Parasaccharibacter apium and Lactobacillus helsingborgensis for PDO honeys and Paenibacillus larvae, Lactobacillus apinorum and Klebsiella pneumoniae for non-PDO honeys. In addition, potential microbial biomarkers of some honey varieties including L. kunkeei for Albaida and Retama del Teide varieties, and P. apium for Tajinaste variety, were identified. ML models were validated on an independent set of samples leading to high accuracy rates (above 90 %). This work demonstrates the potential of ML to differentiate different types of honey using metagenome-based methods, leading to high performance metrics. In addition, ML models discriminate both the geographical origin and variety of products corresponding to different PDOs and non-PDO products. Results here presented may contribute to develop enhanced traceability and authenticity methods that could be applied to a wide range of foods.


Assuntos
Mel , Aprendizado de Máquina , Metagenômica , Mel/análise , Mel/microbiologia , Metagenômica/métodos , Espanha , Bactérias/genética , Bactérias/classificação , Bactérias/isolamento & purificação , Microbiologia de Alimentos , Contaminação de Alimentos/análise
9.
Environ Sci Pollut Res Int ; 31(30): 43037-43048, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38888827

RESUMO

Honeybees are insects very sensitive to environmental pollution and at the same time very good indicators of the pollution levels for certain types of pollutants. The morphology and ethology of these insects make them perfect vectors for dust and substances, including heavy metals produced by anthropic activities or naturally generated and deposited on foraged flora. When bees are raised to produce foods such as honey and pollen, they can easily transfer pollutants collected from contaminated flower affecting the quality of these products. However, depending on geographical location of the apiaries and their distance from pollution sources, the risk to contaminate bee products can be higher or lower requiring deep investigations. In this study, two apiaries were built near ground transport infrastructures and used as monitoring stations for investigating heavy metal presence in beehive products such as bee wax, pollen, and honey. Another apiary was placed between these two locations at a distance of 500 m from each one and used as central node to asses possible diffusion trends. Parallel, air quality was monitored in the proximity of each apiary to verify the air pollution of the environments close to these sites. The results of the study suggest that the presence of the highway and the train station affected the levels of heavy metal presence in the apiary products. Air quality near apiaries was also negatively affected by ground transport, especially in proximity of the highway. Wax resulted significantly more polluted in the apiary close to train station with elements such as Al, Zn, and Ni, while honey and pollen were significantly more polluted in the proximity of the highway with elements such as Al, Fe, Cu, and Zn. Honey was the product suffering less the contamination by heavy metals while pollen was the worse. In conclusion, the presence of transportation nodes determined a higher accumulation of heavy metals in beehive products respect the apiary placed in between, suggesting to pay particular attention in the site selection for the placement of apiaries to protect both bees and human health.


Assuntos
Monitoramento Ambiental , Mel , Metais Pesados , Abelhas , Metais Pesados/análise , Animais , Mel/análise , Pólen/química
10.
Food Chem ; 455: 139876, 2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-38823143

RESUMO

Enrofloxacin (ENR) residues in animal-derived food and water threaten human health. Simple, low-cost and on-site detection methods are urgently needed. Blue emitting carbon quantum dots (CQDs) and orange rhodamine B (RhB) were used as recognition and reference signals, respectively, to construct a ratiometric fluorescence sensor. After the addition of ENR, the color of the sensor changed from orange to blue because hydrogen bonding induced a considerable increase in CQDs fluorescence. Based on this mechanism, a simple and low cost on-site portable sensing platform was constructed, which integrated a stable UV light strip and a smartphone with voice-controlled phototaking function and an RGB app. The t-test results of spiked ENR recoveries for diluted milk, honey and drinking water revealed no significant differences between the ratiometric fluorescent sensor and portable sensing platform. Thus, this portable sensing platform provides a novel strategy for on-site quantification of quinolone antibiotics in foodstuffs and environmental water.


Assuntos
Antibacterianos , Enrofloxacina , Contaminação de Alimentos , Ligação de Hidrogênio , Leite , Pontos Quânticos , Smartphone , Enrofloxacina/análise , Pontos Quânticos/química , Leite/química , Contaminação de Alimentos/análise , Antibacterianos/análise , Animais , Fluorescência , Poluentes Químicos da Água/análise , Mel/análise , Espectrometria de Fluorescência/instrumentação , Espectrometria de Fluorescência/métodos , Água Potável/análise , Carbono/química , Rodaminas/química
11.
Food Chem ; 455: 139888, 2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-38843712

RESUMO

A novel method has been proposed to determine nine plasticizers in honey samples by gas chromatography-mass spectrometry. An efficient sample treatment was proposed (average analyte recoveries between 77% and 118%) involving a double solvent extraction with ethyl acetate, followed by a clean-up step with florisil. Chromatographic analysis (< 21 min) was performed in an Agilent HP-5MS column under programmed temperature conditions. The greenness of the method was assessed with different tools that classified it as environmentally friendly. The method was validated in terms of selectivity, limits of detection (0.1-3.1 µg kg-1) and quantification (0.2-10.3 µg kg-1), linearity, matrix effect, trueness, and precision (relative standard deviation <9%). An analysis of thirty samples from different sources (commercial or experimental apiaries) revealed the presence of residues of five plasticizers in most of the samples. Finally, health risk assessment was evaluated, and the results indicated no associated health risks for consumers.


Assuntos
Contaminação de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Mel , Plastificantes , Plastificantes/análise , Contaminação de Alimentos/análise , Mel/análise , Química Verde
12.
Nutrients ; 16(12)2024 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-38931204

RESUMO

BACKGROUND: There is a growing consensus that fasting-induced ketosis has beneficial effects on human physiology. Despite these compelling benefits, fasting-induced ketosis raises concerns in some clinicians because it is often inappropriately compared with the pathologic uncontrolled ketone production in diabetic ketoacidosis. The determinants of the inter-individual differences in the intensity of ketosis during long-term fasting is unknown. METHODS: We monitored daily variations in fasting ketonemia, as well as ketonuria, which is less invasive, in a large cohort of 1610 subjects, fasting between 4 and 21 days with the Buchinger Wilhelmi program, minimally supplemented with ~75-250 kcal (daily fruit juice, vegetable soup, and honey). RESULTS: Ketonuria was detected in more than 95% of fasting subjects from day 4 onwards. Subjects consuming only soups, without fruit juice or honey, exhibited reduced caloric intake (72 kcal instead of 236 kcal) and carbohydrate intake (15.6 g instead of 56.5 g), leading to more intense ketonuria. Participants with high ketonuria were, in the majority, males, young, had a higher body weight, and had lower HDL-C and urea values. They had a larger decrease in blood glucose, glycated haemoglobin levels, body weight, and waist circumference. Furthermore, in the high-ketonuria group, a larger increase in blood uric acid concentration was observed. CONCLUSION: Our study showed that long-term fasting triggered ketosis, never reaching pathological levels, and that ketosis is influenced by age, gender, health, and the level of physical activity. Furthermore, it is modulated but not suppressed by minimal carbohydrate intake. Our study paves the way for better understanding how supplementation can modulate the therapeutic effects and tolerability of long-term fasting.


Assuntos
Jejum , Cetose , Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Glicemia/metabolismo , Adulto Jovem , Ingestão de Energia , Mel , Fatores de Tempo , Idoso , Sucos de Frutas e Vegetais , Ácido Úrico/sangue
13.
Nutrients ; 16(12)2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38931295

RESUMO

The use of natural products as alternatives to traditional pharmacological treatments in orthodontics is gaining interest due to their anti-inflammatory, antibacterial, and antioxidant properties. This systematic review synthesizes evidence from clinical trials to evaluate the efficacy of natural products in reducing inflammation and bacterial presence in orthodontic and orthognathic treatment settings. The database search was conducted across PubMed, Scopus, and Embase up to January 2024. The review focused on randomized controlled trials only. The selected studies centered on the anti-inflammatory, antibacterial, and antioxidant effects of natural products, adhering to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines for data extraction. Nine studies, totaling 358 participants, were included. Significant findings demonstrated a reduction in gingival inflammation by over 40% with the use of Aloe vera compared to chlorhexidine. Another study noted a decrease in bleeding on probing by 13.6 points in the treatment group over placebo. Additionally, honey showed a rapid modulation of plaque pH and significantly reduced bacterial counts of Streptococcus mutans. Furthermore, the use of resveratrol emulgel was linked to substantial improvements in gingival health, with a reduction in the gingival index and probing pocket depth. The results indicate that natural products can significantly enhance orthodontic treatment outcomes by reducing inflammation and bacterial levels. These products offer effective alternatives to traditional treatments and show potential for integration into routine orthodontic care protocols. Further research is encouraged to standardize application methods and dosages to maximize clinical benefits and patient satisfaction.


Assuntos
Antioxidantes , Produtos Biológicos , Deformidades Dentofaciais , Humanos , Aloe , Antibacterianos/uso terapêutico , Anti-Inflamatórios , Produtos Biológicos/uso terapêutico , Clorexidina , Deformidades Dentofaciais/cirurgia , Deformidades Dentofaciais/tratamento farmacológico , Gengivite/tratamento farmacológico , Mel , Ortodontia/métodos , Preparações de Plantas , Ensaios Clínicos Controlados Aleatórios como Assunto , Resveratrol/farmacologia , Streptococcus mutans/efeitos dos fármacos , Resultado do Tratamento
14.
Prev Vet Med ; 229: 106241, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38878496

RESUMO

Oral vaccination is one of the most effective interventions for disease control in wildlife. As a result of the recent global reemergence of African swine fever and ongoing classical swine fever and animal tuberculosis, oral vaccination of Eurasian wild boar (Sus scrofa) receives increased interest. Several baits for wild boar and feral pigs have been described, but developing more stable and personalized formulations is important. This paper proposes a new bait formulation primarily composed of corn flour, piglet feed, sugar, and honey as a binder to obtain improved elasticity. The bait consists of a matrix with no protective coats, has a hemispherical shape (ø 3.4 ×1.6 cm), and displays an anise aroma and blue color. The color and aroma did not affect bait choice by wild boar, while bait coloring contributed to avoid consumption by non-target species (corvids). Baits with the new formulation were significantly more resistant to humidity and high temperatures than previous versions. Simulations suggest that baits with the new formulation are elastic enough to resist impacts from a maximum altitude of 750 m. Thus, the new bait prototype solves several problems of previous bait formulations while keeping a format that can be selectively consumed by piglets and adult wild boar.


Assuntos
Sus scrofa , Animais , Administração Oral , Suínos , Vacinação/veterinária , Vacinação/métodos , Ração Animal/análise , Vacinas/administração & dosagem , Mel/análise , Zea mays , Animais Selvagens , Açúcares
15.
Food Funct ; 15(13): 7200-7213, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38896046

RESUMO

Colorectal cancer often leads to metastasis, with cancer stem cells (CSCs) playing a pivotal role in this process. Two closely linked mechanisms, epithelial-mesenchymal transition and angiogenesis, contribute to metastasis and recent research has also highlighted the impact of telomere replication on this harmful tumor progression. Standard chemotherapy alone can inadvertently promote drug-resistant CSCs, posing a challenge. Combining chemotherapy with other compounds, including natural ones, shows promise in enhancing effectiveness while minimizing side effects. This study investigated the anti-metastatic potential of Manuka honey, both alone and in combination with 5-fluorouracil, using a 3D model of colonospheres enriched with CSC-like cells. In summary, it was observed that the treatment reduced migration ability by downregulating the transcription factors Slug, Snail, and Twist, which are key players in epithelial-mesenchymal transition. Additionally, Manuka honey downregulated pro-angiogenic factors and shortened CSC telomeres by downregulating c-Myc - demonstrating an effective anti-metastatic potential. This study suggests new research opportunities for studying the impact of natural compounds when combined with pharmaceuticals, with the potential to enhance effectiveness and reduce side effects.


Assuntos
Neoplasias do Colo , Transição Epitelial-Mesenquimal , Mel , Células-Tronco Neoplásicas , Neovascularização Patológica , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Humanos , Células-Tronco Neoplásicas/efeitos dos fármacos , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/genética , Neovascularização Patológica/tratamento farmacológico , Linhagem Celular Tumoral , Fatores de Transcrição da Família Snail/metabolismo , Fatores de Transcrição da Família Snail/genética , Fluoruracila/farmacologia , Leptospermum/química , Movimento Celular/efeitos dos fármacos , Metástase Neoplásica , Telômero/efeitos dos fármacos , Homeostase do Telômero/efeitos dos fármacos , Angiogênese
16.
J Coll Physicians Surg Pak ; 34(6): 693-696, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38840353

RESUMO

OBJECTIVE: To evaluate the effect of Ziziphus honey on the healing of post-extraction alveolar sockets by estimating the levels of osteopontin (OPN) in humans. STUDY DESIGN: Randomised controlled trial. Place and Duration of the Study: Dental section of the Lahore General Hospital, Lahore, Pakistan, from March 2020 to February 2021. METHODOLOGY: A total of 30 patients were included in the study. The mean age was 35 ± 0.28 years. The participants were adults undergoing permanent molar extraction, randomly divided into two groups, a control group and an experimental group. After tooth extractions in both groups, 1ml of Ziziphus honey was administered into the extracted tooth socket of the experimental group while no intervention was done to the control group. Saliva samples were collected on day 0 before tooth extraction and on days 3 and 7 after tooth extractions. Enzyme-linked immunosorbent assay (ELISA) technique was used to measure the levels of OPN in the saliva sample. Radiographic evaluation was also done with the help of periapical radiographs using Image J® software. To find out the significance of the outcome in experimental and control groups, an unpaired t-test was applied. A p-value <0.05 was considered statistically significant. RESULTS: A total of 30 participants were selected for the study, of which 16 were females and 14 were males. The OPN levels between the control vs. experimental groups were (22.55 ± 2.45 vs. 23.31 ± 2.38; p = 0.4) on day 0, (30.95 ± 2.96 vs. 53.29 ± 4.69; p = 0.001) on day 3, and (55.33 ± 4.52 vs. 81.90 ± 4.49; p = 0.001) on day 7. CONCLUSION: Increased salivary levels of the OPN in the experimental group with the use of Ziziphus honey suggests better bone healing as compared to the control group. KEY WORDS: Extraction tooth, Honey, Osteopontin, Ziziphus, Bone healing.


Assuntos
Mel , Osteopontina , Saliva , Extração Dentária , Alvéolo Dental , Cicatrização , Humanos , Osteopontina/metabolismo , Osteopontina/análise , Masculino , Feminino , Adulto , Saliva/química , Saliva/metabolismo , Cicatrização/fisiologia , Paquistão
17.
Front Cell Infect Microbiol ; 14: 1380289, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38868298

RESUMO

The antibiotic resistance (ABR) crisis is an urgent global health priority. Staphylococci are among the problematic bacteria contributing to this emergency owing to their recalcitrance to many clinically important antibiotics. Staphylococcal pathogenesis is further complicated by the presence of small colony variants (SCVs), a bacterial subpopulation displaying atypical characteristics including retarded growth, prolific biofilm formation, heightened antibiotic tolerance, and enhanced intracellular persistence. These capabilities severely impede current chemotherapeutics, resulting in chronic infections, poor patient outcomes, and significant economic burden. Tackling ABR requires alternative measures beyond the conventional options that have dominated treatment regimens over the past 8 decades. Non-antibiotic therapies are gaining interest in this arena, including the use of honey, which despite having ancient therapeutic roots has now been reimagined as an alternative treatment beyond just traditional topical use, to include the treatment of an array of difficult-to-treat staphylococcal infections. This literature review focused on Manuka honey (MH) and its efficacy as an anti-staphylococcal treatment. We summarized the studies that have used this product and the technologies employed to study the antibacterial mechanisms that render MH a suitable agent for the management of problematic staphylococcal infections, including those involving staphylococcal SCVs. We also discussed the status of staphylococcal resistance development to MH and other factors that may impact its efficacy as an alternative therapy to help combat ABR.


Assuntos
Antibacterianos , Mel , Infecções Estafilocócicas , Staphylococcus , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/microbiologia , Staphylococcus/efeitos dos fármacos , Humanos , Antibacterianos/farmacologia , Fenótipo , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Animais , Leptospermum/química , Testes de Sensibilidade Microbiana
18.
J Foot Ankle Res ; 17(2): e12032, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38884388

RESUMO

INTRODUCTION: Diabetic foot ulcers (DFUs) are commonly contaminated with pathogenic organisms and precede most diabetes-related amputations. Antimicrobial dressings are used in the treatment of DFUs; however, recent guidelines do not support their use. There are no data describing the experience of antimicrobial dressing use among podiatrists in Aotearoa New Zealand (AoNZ). This study aimed to (i) determine which antimicrobial dressings podiatrists in AoNZ use for the management of diabetic foot ulcers; and (ii) determine what factors influence AoNZ podiatrists' use of antimicrobial dressing when managing DFUs. METHODS: An anonymous cross-sectional web-based survey was undertaken. Participants were AoNZ registered podiatrists who managed DFUs in their practice. The survey included questions relating to personal and professional demographic characteristics and DFU management and dressing practices. Descriptive statistics were computed to address the research aims. RESULTS: Responses from 43 AoNZ podiatrists were included. Participants reported both cadexomer iodine and silver dressings were the most common antimicrobial dressings used, with honey dressings being the least frequently used. The most influential factors in choosing antimicrobial dressings when managing DFUs were the presence of current infection, ulcer exudate and ability to prevent future infection. The least influential factors in choosing antimicrobial dressings when managing DFUs were patient preferences, cost of dressings and comfort of dressing/pain on removal. CONCLUSIONS: AoNZ podiatrists managing DFUs primarily use antimicrobial dressings containing cadexomer iodine or silver as active ingredients, while lower-cost options, such as honey and povidone iodine are less often used. Current recommendations highlight the lack of evidence to support positive outcomes from any particular antimicrobial dressing over another and advocate that exudate control, comfort and cost be prioritised in decision-making. As cost has been an increasing burden to our healthcare funding, clinicians and organisations may consider this before purchasing and stocking expensive dressings.


Assuntos
Bandagens , Pé Diabético , Podiatria , Padrões de Prática Médica , Humanos , Pé Diabético/terapia , Pé Diabético/tratamento farmacológico , Nova Zelândia , Estudos Transversais , Bandagens/economia , Bandagens/estatística & dados numéricos , Podiatria/estatística & dados numéricos , Masculino , Feminino , Padrões de Prática Médica/estatística & dados numéricos , Pessoa de Meia-Idade , Anti-Infecciosos/uso terapêutico , Anti-Infecciosos/economia , Inquéritos e Questionários , Adulto , Mel
19.
Pak J Pharm Sci ; 37(2(Special)): 429-434, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38822546

RESUMO

Dental implants are commonly used for tooth replacement tools due to their good oral rehabilitation and reconstruction capacities. Dental implants treatment for natural teeth is desired to achieve successful implants treatment with improved osseointegration through promotion of mammalian cell activity and prevention of bacterial activity. Honey is potentially known for its antimicrobial and antibacterial potential, specifically for burns and wound healing. In this study, honey based silver nanoparticles were synthesized using various concentrations of honey. The synthesized HNY-AgNPs, MSN and HNY-AgMSN were characterized for their surface Plasmon resonance using UV spectroscopy, Hydrodynamic diameter using Zetasizer. Morphology using AFM. Furthermore, surface functional groups were characterized using FTIR spectroscopy at 4cm-1 resolutions. The developed hybrid nanoparticles were tested for their anti-bacterial activity at concentration of 3000µg/mL. It was found HNY-AgNPs was active against both bacterial strains i.e, Streptococcus mutans and streptococcus aureus. HNY-AgNPs-MSN hybrid implant demonstrated potential new type of dental implants, which can offer an effective design for the fabrication of advanced dental implants.


Assuntos
Antibacterianos , Implantes Dentários , Mel , Nanopartículas Metálicas , Prata , Streptococcus mutans , Antibacterianos/farmacologia , Antibacterianos/síntese química , Prata/química , Prata/farmacologia , Streptococcus mutans/efeitos dos fármacos , Nanopartículas Metálicas/química , Testes de Sensibilidade Microbiana , Staphylococcus aureus/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier
20.
Sci Rep ; 14(1): 14122, 2024 06 19.
Artigo em Inglês | MEDLINE | ID: mdl-38898099

RESUMO

Southern Asian flowers offer honeybees a diversity of nectar. Based on its geographical origin, honey quality varies. Traditional methods are less authentic than DNA-based identification. The origin of honey is determined by pollen, polyphenolic, and macro-microorganisms. In this study, amplicon sequencing targets macro-microorganisms in eDNA using the ITS1 region to explore honey's geographical location and authentication. The variety of honey samples was investigated using ITS1 with Illumina sequencing. For all four honey samples, raw sequence reads showed 979,380 raw ITS1 amplicon reads and 375 ASVs up to the phylum level. The highest total number of 202 ASVs up to phylum level identified Bali honey with 211,189 reads, followed by Banggi honey with 309,207 a total number of 111 ASVs, and Lombok represents only 63 ASVs up to phylum level with several read 458,984. Based on Shannon and Chao1, honey samples from Bali (B2) and (B3) exhibited higher diversity than honey from Lombok (B1) and green honey from Sabah (B4), while the Simpson index showed that Banggi honey (B4) had higher diversity. Honey samples had significant variance in mycobiome taxonomic composition and abundance. Zygosaccharomyces and Aspergillus were the main genera found in Lombok honey, with percentages of 68.81% and 29.76% respectively. Bali honey samples (B2 and B3) were identified as having a significant amount of the genus Aureobasidium, accounting for 40.81% and 25% of the readings, respectively. The microbiome composition of Banggi honey (B4) showed a high presence of Zygosaccharomyces 45.17% and Aureobasidium 35.24%. The ITS1 analysis effectively distinguishes between honey samples of different origins and its potential as a discriminatory tool for honey origin and authentication purposes.


Assuntos
Mel , Mel/análise , Abelhas/genética , Abelhas/microbiologia , Animais , Micobioma/genética , Sudeste Asiático , DNA Intergênico/genética , Fungos/genética , Fungos/classificação , Fungos/isolamento & purificação , Pólen , Ilhas , População do Sudeste Asiático
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA