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1.
Prog Orthod ; 25(1): 22, 2024 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-38825612

RESUMO

BACKGROUND: The aim of the present study was to investigate qualitatively and quantitatively the elution of substances from polyester-urethane (Invisalign™) aligners and resin composite attachments (Tetric EvoFlow) in vivo. METHODS: Patients (n = 11) treated with the aligners and attachments (16 per patient, without other composite restorations) for an average of 20 months, who were planned for attachment removed were enrolled in the study. Patients were instructed to rinse with 50 mL of distilled water upon entry and the rinsing solution was collected (before removal). Then, the attachments were removed with low-speed tungsten carbide burs for adhesive residue removal, a thorough water rinsing was performed immediately after the grinding process to discard grinding particle residues, and subsequently, after a second water-rinsing the solution was collected for analysis (after removal). The rinsing solutions were analyzed for targeted (LC-MS/MS: Bis-GMA, DCDMA, UDMA, BPA) and untargeted (LC-HRMS: screening of leached species and their degradation products) compounds. RESULTS: Targeted analysis revealed a significant reduction in BPA after attachment removal (4 times lower). Bis-GMA, DCDMA, UDMA were below the detection limit before removal but were all detectable after removal with Bis-GMA and UDMA at quantifiable levels. Untargeted analysis reviled the presence of mono-methacrylate transformation products of Bis-GMA (Bis-GMA-M1) and UDMA (UDMA-M1), UDMA without methacrylate moieties (UDMA-M2), and 4-(dimethylamino) benzoic acid (DMAB), the degradation product of the photo-initiator ethyl-4-(dimethylamino) benzoate (EDMAB), all after attachment removal. Several amino acids and endogenous metabolites were also found both before and after removal. CONCLUSIONS: Elevated levels of BPA were traced instantaneously in patients treated with Invisalign™ and flowable resin composite attachments for the testing period. BPA was reduced after attachment removal, but residual monomers and resin degradation products were found after removal. Alternative resin formulations and attachment materials may be utilized to reduce eluents.


Assuntos
Resinas Compostas , Metacrilatos , Poliuretanos , Humanos , Poliuretanos/química , Resinas Compostas/química , Feminino , Masculino , Metacrilatos/química , Saliva/química , Bis-Fenol A-Glicidil Metacrilato/química , Adulto , Aparelhos Ortodônticos Removíveis , Poliésteres/química , para-Aminobenzoatos/análise , Adulto Jovem , Adolescente , Técnicas de Movimentação Dentária/instrumentação , Técnicas de Movimentação Dentária/métodos , Espectrometria de Massas em Tandem , Cromatografia Líquida
2.
PLoS One ; 19(6): e0301618, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38843277

RESUMO

Periprosthetic tissue inflammation is a challenging complication arising in joint replacement surgeries, which is often caused by wear debris from polyethylene (PE) components. In this study, we examined the potential biological effects of grafting a [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (MEDSAH) polymer onto the surface of PE through a solvent-evaporation technique. J774A.1 macrophage-like cells and primary cultured mouse osteoblasts were treated with PE powder with or without the MEDSAH coating. MEDSAH grafting on PE substantially reduced the expression of pro-inflammatory cytokines and other mediators in primary cultured mouse osteoblasts, but did not significantly impact macrophage-mediated inflammation. Our findings suggest that a MEDSAH coating on PE-based materials has potential utility in mitigating periprosthetic tissue inflammation and osteolysis and preventing aseptic loosening in total joint replacements. Further research, including large-scale clinical trials and biomechanical analyses, is needed to assess the long-term performance and clinical implications of MEDSAH-coated PE-based materials in total joint arthroplasty.


Assuntos
Inflamação , Osteoblastos , Polietileno , Animais , Camundongos , Inflamação/patologia , Osteoblastos/metabolismo , Osteoblastos/efeitos dos fármacos , Macrófagos/metabolismo , Linhagem Celular , Citocinas/metabolismo , Osteólise/etiologia , Osteólise/patologia , Materiais Revestidos Biocompatíveis/química , Metacrilatos/química , Artroplastia de Substituição/efeitos adversos
3.
Carbohydr Polym ; 339: 122253, 2024 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-38823920

RESUMO

In vitro tumor models are essential for understanding tumor behavior and evaluating tumor biological properties. Hydrogels that can mimic the tumor extracellular matrix have become popular for creating 3D in vitro tumor models. However, designing biocompatible hydrogels with appropriate chemical and physical properties for constructing tumor models is still a challenge. In this study, we synthesized a series of ß-cyclodextrin (ß-CD)-crosslinked polyacrylamide hydrogels with different ß-CD densities and mechanical properties and evaluated their potential for use in 3D in vitro tumor model construction, including cell capture and spheroid formation. By utilizing a combination of ß-CD-methacrylate (CD-MA) and a small amount of N,N'-methylene bisacrylamide (BIS) as hydrogel crosslinkers and optimizing the CD-MA/BIS ratio, the hydrogels performed excellently for tumor cell 3D culture and spheroid formation. Notably, when we co-cultured L929 fibroblasts with HeLa tumor cells on the hydrogel surface, co-cultured spheroids were formed, showing that the hydrogel can mimic the complexity of the tumor extracellular matrix. This comprehensive investigation of the relationship between hydrogel mechanical properties and biocompatibility provides important insights for hydrogel-based in vitro tumor modeling and advances our understanding of the mechanisms underlying tumor growth and progression.


Assuntos
Resinas Acrílicas , Hidrogéis , Esferoides Celulares , beta-Ciclodextrinas , Esferoides Celulares/efeitos dos fármacos , Humanos , Resinas Acrílicas/química , Resinas Acrílicas/farmacologia , Hidrogéis/química , Hidrogéis/farmacologia , Hidrogéis/síntese química , beta-Ciclodextrinas/química , beta-Ciclodextrinas/farmacologia , Células HeLa , Animais , Camundongos , Reagentes de Ligações Cruzadas/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Técnicas de Cultura de Células em Três Dimensões/métodos , Metacrilatos/química , Técnicas de Cocultura , Neoplasias/patologia
4.
Sci Rep ; 14(1): 12670, 2024 06 03.
Artigo em Inglês | MEDLINE | ID: mdl-38830883

RESUMO

Gelatin-methacryloyl (GelMA) is a highly adaptable biomaterial extensively utilized in skin regeneration applications. However, it is frequently imperative to enhance its physical and biological qualities by including supplementary substances in its composition. The purpose of this study was to fabricate and characterize a bi-layered GelMA-gelatin scaffold using 3D bioprinting. The upper section of the scaffold was encompassed with keratinocytes to simulate the epidermis, while the lower section included fibroblasts and HUVEC cells to mimic the dermis. A further step involved the addition of amniotic membrane extract (AME) to the scaffold in order to promote angiogenesis. The incorporation of gelatin into GelMA was found to enhance its stability and mechanical qualities. While the Alamar blue test demonstrated that a high concentration of GelMA (20%) resulted in a decrease in cell viability, the live/dead cell staining revealed that incorporation of AME increased the quantity of viable HUVECs. Further, gelatin upregulated the expression of KRT10 in keratinocytes and VIM in fibroblasts. Additionally, the histological staining results demonstrated the formation of well-defined skin layers and the creation of extracellular matrix (ECM) in GelMA/gelatin hydrogels during a 14-day culture period. Our study showed that a 3D-bioprinted composite scaffold comprising GelMA, gelatin, and AME can be used to regenerate skin tissues.


Assuntos
Âmnio , Bioimpressão , Fibroblastos , Gelatina , Células Endoteliais da Veia Umbilical Humana , Queratinócitos , Engenharia Tecidual , Alicerces Teciduais , Queratinócitos/efeitos dos fármacos , Queratinócitos/citologia , Queratinócitos/metabolismo , Gelatina/química , Humanos , Engenharia Tecidual/métodos , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Fibroblastos/citologia , Alicerces Teciduais/química , Âmnio/citologia , Âmnio/metabolismo , Âmnio/química , Bioimpressão/métodos , Impressão Tridimensional , Pele/metabolismo , Pele/citologia , Metacrilatos/química , Sobrevivência Celular/efeitos dos fármacos , Células Endoteliais/metabolismo , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/citologia
5.
Int J Biol Macromol ; 272(Pt 1): 132856, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38834118

RESUMO

Economically and efficiently removing organic pollutants from water is still a challenge in wastewater treatment. Utilizing environmentally friendly and readily available protein-based natural polymers to develop aerogels with effective removal performance and sustainable regeneration capability is a promising strategy for adsorbent design. Here, a robust and cost-effective method using inexpensive ß-lactoglobulin (BLG) as raw material was proposed to fabricate BLG-based aerogels. Firstly, photocurable BLG-based polymers were synthesized by grafting glycidyl methacrylate. Then, a cross-linking reaction, including photo-crosslinking and salting-out treatment, was applied to prepared BLG-based hydrogels. Finally, the BLG-based aerogels with high porosity and ultralight weight were obtained after freeze-drying. The outcomes revealed that the biocompatible BLG-based aerogels exhibited effective removal performance for a variety of organic pollutants under perfectly quiescent conditions, and could be regenerated and reused many times via a simple and rapid process of acid washing and centrifugation. Overall, this work not only demonstrates that BLG-based aerogels are promising adsorbents for water purification but also provides a potential way for the sustainable utilization of BLG.


Assuntos
Géis , Lactoglobulinas , Poluentes Químicos da Água , Purificação da Água , Lactoglobulinas/química , Lactoglobulinas/isolamento & purificação , Poluentes Químicos da Água/isolamento & purificação , Poluentes Químicos da Água/química , Purificação da Água/métodos , Géis/química , Adsorção , Porosidade , Hidrogéis/química , Água/química , Compostos de Epóxi , Metacrilatos
6.
Biomed Mater ; 19(4)2024 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-38838692

RESUMO

At present, wound dressings in clinical applications are primarily used for superficial skin wounds. However, these dressings have significant limitations, including poor biocompatibility and limited ability to promote wound healing. To address the issue, this study used aldehyde polyethylene glycol as the cross-linking agent to design a carboxymethyl chitosan-methacrylic acid gelatin hydrogel with enhanced biocompatibility, which can promote wound healing and angiogenesis. The CSDG hydrogel exhibits acid sensitivity, with a swelling ratio of up to 300%. Additionally, it exhibited excellent resistance to external stress, withstanding pressures of up to 160 kPa and self-deformation of 80%. Compared to commercially available chitosan wound gels, the CSDG hydrogel demonstrates excellent biocompatibility, antibacterial properties, and hemostatic ability. Bothin vitroandin vivoresults showed that the CSDG hydrogel accelerated blood vessel regeneration by upregulating the expression of CD31, IL-6, FGF, and VEGF, thereby promoting rapid healing of wounds. In conclusion, this study successfully prepared the CSDG hydrogel wound dressings, providing a new approach and method for the development of hydrogel dressings based on natural macromolecules.


Assuntos
Materiais Biocompatíveis , Quitosana , Gelatina , Hidrogéis , Metacrilatos , Cicatrização , Quitosana/química , Quitosana/análogos & derivados , Cicatrização/efeitos dos fármacos , Gelatina/química , Hidrogéis/química , Animais , Metacrilatos/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Camundongos , Humanos , Polietilenoglicóis/química , Antibacterianos/química , Antibacterianos/farmacologia , Neovascularização Fisiológica/efeitos dos fármacos , Bandagens , Masculino , Reagentes de Ligações Cruzadas/química , Regeneração/efeitos dos fármacos , Hemostáticos/química , Hemostáticos/farmacologia , Teste de Materiais , Ratos
7.
Biomed Mater ; 19(4)2024 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-38838701

RESUMO

Although different fabrication methods and biomaterials are used in scaffold development, hydrogels and electrospun materials that provide the closest environment to the extracellular matrix have recently attracted considerable interest in tissue engineering applications. However, some of the limitations encountered in the application of these methods alone in scaffold fabrication have increased the tendency to use these methods together. In this study, a bilayer scaffold was developed using 3D-printed gelatin methacryloyl (GelMA) hydrogel containing ciprofloxacin (CIP) and electrospun polycaprolactone (PCL)-collagen (COL) patches. The bilayer scaffolds were characterized in terms of chemical, morphological, mechanical, swelling, and degradation properties; drug release, antibacterial properties, and cytocompatibility of the scaffolds were also studied. In conclusion, bilayer GelMA-CIP/PCL-COL scaffolds, which exhibit sufficient porosity, mechanical strength, and antibacterial properties and also support cell growth, are promising potential substitutes in tissue engineering applications.


Assuntos
Antibacterianos , Materiais Biocompatíveis , Ciprofloxacina , Gelatina , Hidrogéis , Teste de Materiais , Metacrilatos , Poliésteres , Impressão Tridimensional , Engenharia Tecidual , Alicerces Teciduais , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Gelatina/química , Ciprofloxacina/farmacologia , Ciprofloxacina/química , Poliésteres/química , Antibacterianos/farmacologia , Antibacterianos/química , Materiais Biocompatíveis/química , Hidrogéis/química , Porosidade , Metacrilatos/química , Colágeno/química , Animais , Humanos , Proliferação de Células/efeitos dos fármacos
8.
Eur J Orthod ; 46(4)2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38884540

RESUMO

AIM: The aim of the present study was to assess the alterations in morphology, roughness, and composition of the surfaces of a conventional and a flowable composite attachment engaged with aligners, and to evaluate the release of resin monomers and their derivatives in an aqueous environment. METHODS: Zirconia tooth-arch frames (n = 20) and corresponding thermoformed PET-G aligners with bonded attachments comprising two composite materials (universal-C and flowable-F) were fabricated. The morphological features (stereomicroscopy), roughness (optical profilometry), and surface composition (ATR-FTIR) of the attachments were examined before and after immersion in water. To simulate intraoral use, the aligners were removed and re-seated to the frames four times per day for a 7-day immersion period. After testing, the eluents were analyzed by LC-MS/MS targeting the compounds Bis-GMA, UDMA, 2-HEMA, TEGDMA and BPA and by LC-HRMS for suspect screening of the leached dental material compounds and their degradation products. RESULTS: After testing, abrasion-induced defects were found on attachment surfaces such as scratches, marginal cracks, loss of surface texturing, and fractures. The morphological changes and debonding rate were greater in F. Comparisons (before-after testing) revealed a significantly lower Sc roughness parameter in F. The surface composition of the aligners after testing showed minor changes from the control, with insignificant differences in the degree of C = C conversion, except for few cases with strong evidence of hydrolytic degradation. Targeted analysis results revealed a significant difference in the compounds released between Days 1 and 7 in both materials. Insignificant differences were found when C was compared with F in both timeframes. Several degradation products were detected on Day 7, with a strong reduction in the concentration of the targeted compounds. CONCLUSIONS: The use of aligners affects the surface characteristics and degradation rate of composite attachments in an aqueous environment, releasing monomers, and monomer hydrolysates within 1-week simulated use.


Assuntos
Resinas Compostas , Teste de Materiais , Metacrilatos , Propriedades de Superfície , Zircônio , Zircônio/química , Resinas Compostas/química , Metacrilatos/química , Polietilenoglicóis/química , Ácidos Polimetacrílicos/química , Poliuretanos/química , Bis-Fenol A-Glicidil Metacrilato/química , Materiais Dentários/química , Técnicas In Vitro , Humanos , Técnicas de Movimentação Dentária/instrumentação , Técnicas de Movimentação Dentária/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos
9.
Biomed Mater ; 19(4)2024 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-38768611

RESUMO

Gelatin methacryloyl (GelMA) hydrogels have gained significant recognition as versatile biomaterials in the biomedical domain. GelMA hydrogels emulate vital characteristics of the innate extracellular matrix by integrating cell-adhering and matrix metalloproteinase-responsive peptide motifs. These features enable cellular proliferation and spreading within GelMA-based hydrogel scaffolds. Moreover, GelMA displays flexibility in processing, as it experiences crosslinking when exposed to light irradiation, supporting the development of hydrogels with adjustable mechanical characteristics. The drug delivery landscape has been reshaped by GelMA hydrogels, offering a favorable platform for the controlled and sustained release of therapeutic actives. The tunable physicochemical characteristics of GelMA enable precise modulation of the kinetics of drug release, ensuring optimal therapeutic effectiveness. In tissue engineering, GelMA hydrogels perform an essential role in the design of the scaffold, providing a biomimetic environment conducive to cell adhesion, proliferation, and differentiation. Incorporating GelMA in three-dimensional printing further improves its applicability in drug delivery and developing complicated tissue constructs with spatial precision. Wound healing applications showcase GelMA hydrogels as bioactive dressings, fostering a conducive microenvironment for tissue regeneration. The inherent biocompatibility and tunable mechanical characteristics of GelMA provide its efficiency in the closure of wounds and tissue repair. GelMA hydrogels stand at the forefront of biomedical innovation, offering a versatile platform for addressing diverse challenges in drug delivery, tissue engineering, and wound healing. This review provides a comprehensive overview, fostering an in-depth understanding of GelMA hydrogel's potential impact on progressing biomedical sciences.


Assuntos
Materiais Biocompatíveis , Gelatina , Hidrogéis , Metacrilatos , Animais , Humanos , Materiais Biocompatíveis/química , Adesão Celular , Proliferação de Células , Sistemas de Liberação de Medicamentos , Matriz Extracelular/metabolismo , Gelatina/química , Hidrogéis/química , Metacrilatos/química , Impressão Tridimensional , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Cicatrização/efeitos dos fármacos
10.
Int J Pharm ; 658: 124205, 2024 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-38734278

RESUMO

The current wound healing process faces numerous challenges such as bacterial infection, inflammation and oxidative stress. However, wound dressings used to promote wound healing, are not well suited to meet the clinical needs. Hyaluronic acid (HA) not only has excellent water absorption and good biocompatibility but facilitates cell function and tissue regeneration. Dopamine, on the other hand, increases the overall viscosity of the hydrogel and possesses antioxidant property. Furthermore, chitosan exhibits outstanding performance in antimicrobial, anti-inflammatory and antioxidant activities. Basic fibroblast growth factor (bFGF) is conducive to cell proliferation and migration, vascular regeneration and wound healing. Hence, we designed an all-in-one hydrogel patch containing dopamine and chitosan framed by hyaluronic acid (HDC) with sprayed gelatin methacryloyl (GelMA) microspheres loaded with bFGF (HDC-bFGF). The hydrogel patch exhibits excellent adhesive, anti-inflammatory, antioxidant and antibacterial properties. In vitro experiments, the HDC-bFGF hydrogel patch not only showed significant inhibitory effect on RAW cell inflammation and Staphylococcus aureus (S. aureus) growth but also effectively scavenged free radicals, in addition to promoting the migration of 3 T3 cells. In the mice acute infected wound model, the HDC-bFGF hydrogel patch adhered to the wound surface greatly accelerated the healing process via its anti-inflammatory and antioxidant activities, bacterial inhibition and pro-vascularization effects. Therefore, the multifunctional HDC-bFGF hydrogel patch holds great promise for clinical application.


Assuntos
Antibacterianos , Anti-Inflamatórios , Antioxidantes , Quitosana , Fator 2 de Crescimento de Fibroblastos , Gelatina , Hidrogéis , Metacrilatos , Microesferas , Staphylococcus aureus , Cicatrização , Animais , Cicatrização/efeitos dos fármacos , Camundongos , Fator 2 de Crescimento de Fibroblastos/administração & dosagem , Fator 2 de Crescimento de Fibroblastos/química , Fator 2 de Crescimento de Fibroblastos/farmacologia , Gelatina/química , Staphylococcus aureus/efeitos dos fármacos , Antibacterianos/administração & dosagem , Antibacterianos/farmacologia , Antibacterianos/química , Hidrogéis/química , Hidrogéis/administração & dosagem , Quitosana/química , Quitosana/administração & dosagem , Antioxidantes/administração & dosagem , Antioxidantes/farmacologia , Antioxidantes/química , Metacrilatos/química , Metacrilatos/administração & dosagem , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/química , Masculino , Dopamina/administração & dosagem , Dopamina/química , Dopamina/farmacologia , Ácido Hialurônico/química , Ácido Hialurônico/administração & dosagem , Ácido Hialurônico/farmacologia , Células RAW 264.7 , Movimento Celular/efeitos dos fármacos , Infecção dos Ferimentos/tratamento farmacológico
11.
Dent Mater J ; 43(3): 400-406, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38719585

RESUMO

This study aimed to evaluate the effects of dentin bonding agents and silanization on the bond strength between 3D printed resin and composite resin and compare it with a conventional composite resin. 3D printed resin cylinders (PCB) and composite resin substrates (Z250) were prepared and divided into eight subgroups based on the bonding agents used (n=12). The shear bond strength was measured using a universal testing machine, and the failure modes were evaluated. The bond strength was found to vary significantly among the bonding agents and substrate types. Silane application did not significantly improve the bond strength. Among the bonding agents, the universal adhesives exhibited the highest bond strengths for both substrates. Compared to PCB, Z250 demonstrated stronger bonds and exhibited more cohesive failures. Further research is needed to optimize the surface treatments and resin formulations for enhanced bond strength and durability between 3D printed and composite resins.


Assuntos
Resinas Compostas , Colagem Dentária , Análise do Estresse Dentário , Adesivos Dentinários , Teste de Materiais , Impressão Tridimensional , Resistência ao Cisalhamento , Silanos , Propriedades de Superfície , Resinas Compostas/química , Adesivos Dentinários/química , Silanos/química , Colagem Dentária/métodos , Cimentos de Resina/química , Metacrilatos/química
12.
Int J Biol Macromol ; 270(Pt 1): 132310, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38740162

RESUMO

With multiscale hierarchical structure, wood is suitable for a range of high-value applications, especially as a chromatographic matrix. Here, we have aimed to provide a weak anion-exchange polymeric monolithic column based on natural wood with high permeability and stability for effectively separating the targeted protein. The wood-polymeric monolithic column was synthesized by in situ polymerization of glycidyl methacrylate and ethylene glycol dimethacrylate in wood, and coupled with diethylaminoethyl hydrochloride. The wood-polymeric monolithic column can be integrated with fast-protein liquid chromatography for large-scale protein purification. According to the results, the wood-polymeric monolithic column showed high hydrophilicity, permeability and stability. Separation experiments verified that the wood-polymeric monolithic column could purify the targeted protein (spike protein of SARS-COV-2 and ovalbumin) from the mixed proteins by ion exchange, and the static adsorption capacity was 33.04 mg mL-1 and the dynamic adsorption capacity was 24.51 mg mL-1. In addition, the wood-polymerized monolithic column had good stability, and a negligible decrease in the dynamic adsorption capacity after 20 cycles. This wood-polymerized monolithic column can provide a novel, efficient, and green matrix for monolithic chromatographic columns.


Assuntos
Madeira , Madeira/química , Adsorção , Metacrilatos/química , Cromatografia por Troca Iônica/métodos , Polímeros/química , Ovalbumina/química , Ovalbumina/isolamento & purificação , Interações Hidrofóbicas e Hidrofílicas , SARS-CoV-2 , Polimerização , Compostos de Epóxi
13.
Int J Biol Macromol ; 270(Pt 1): 132331, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38750843

RESUMO

Most of the transition metal ions and organic dyes are toxic in nature. Therefore, their removal from water is imperative for human health. For this purpose, various types of systems have been developed to tackle either transition metal ions or organic dyes individually. A core-shell microgel system is introduced which is capable of effectively removing both types (toxic organic dyes and transition metal ions) of pollutants. A long-rod-shaped silica@poly(chitosan-N-isopropylacrylamide-methacrylic acid) S@P(CS-NIPAM-MAA) S@P(CNM) core-shell microgel system was developed by free radical precipitation polymerization method (FRPPM). S@P(CNM) was utilized as an adsorbent for extracting palladium (II) (Pd (II)) ions from water under different concentrations of S@P(CNM), several agitation times, palladium (II) ion content, and pH levels. The adsorption data of Pd (II) ions on S@P(CNM) was evaluated by various adsorption isotherms. The kinetic study was investigated by employing pseudo-2nd order (Ps2O), Elovich model (ElM), intra-particle diffusion (IPDM), and pseudo-1st order (Ps1O). Additionally, palladium nanoparticles (Pd NPs) were generated via in-situ reduction of adsorbed Pd (II) ions within the P(CNM) shell region of S@P(CNM). The resulting Pd NPs loaded S@P(CNM) exhibited the capability to reduce organic pollutants like methyl orange (MeO), 4-nitrophenol (4NiP), methylene blue (MeB), and Rhodamine B (RhB) from aqueous medium. 0.766 min-1, 0.433 min-1, 0.682 min-1, and 1.140 min-1 were the values of pseudo 1st order rate constant (kobs) for catalytic reduction of MeB, 4NiP, MeO, and RhB respectively. The S@Pd-P(CNM) system exhibits significant catalytic potential for various organic transformations.


Assuntos
Quitosana , Nanopartículas Metálicas , Paládio , Dióxido de Silício , Poluentes Químicos da Água , Paládio/química , Quitosana/química , Dióxido de Silício/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/isolamento & purificação , Adsorção , Nanopartículas Metálicas/química , Cinética , Acrilamidas/química , Géis/química , Purificação da Água/métodos , Concentração de Íons de Hidrogênio , Metacrilatos/química , Íons/química
14.
ACS Appl Mater Interfaces ; 16(22): 28162-28171, 2024 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-38767334

RESUMO

This study investigated the suitability of surface modification for a long-range surface plasmon (LRSP) aptasensor using two different hydrogels, aiming at real-time monitoring of vancomycin (VCM) in undiluted serum and blood. Three different layer structures were formed on a gold surface of LRSP sensor chip using poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-N-methacryloyl-(L)-tyrosinemethylester (MAT)] (PMM) and poly[MPC-co-2-ethylhexyl methacrylate (EHMA)-co-MAT] (PMEM). The peptide aptamer for VCM was immobilized in PMM and PMEM via MAT. Among four differently prepared sensor chips, the LRSP hydrogel aptasensor with PMM, referred to as the PMM hydrogel, exhibited the highest sensor output and superior antifouling properties. Following the optimization of the PMM hydrogel preparation conditions, the shelf life of the PMM hydrogel was determined to exceed 2 weeks, and the same sensor chip could be used for 102 days without significant performance deterioration. The PMM hydrogel was then applied for VCM measurement in undiluted serum in vitro, where it demonstrated a limit of detection of 0.098 µM and a dynamic range of 0.18-100 µM, covering the therapeutic range. Additionally, the PMM hydrogel enabled the continuous measurement of various VCM concentrations in serum without rinsing and showed a concentration-dependent output in undiluted blood. These findings underscore the potential of the PMM hydrogel for real-time and direct monitoring of VCM in body fluids.


Assuntos
Hidrogéis , Ressonância de Plasmônio de Superfície , Vancomicina , Vancomicina/sangue , Vancomicina/química , Vancomicina/farmacologia , Humanos , Hidrogéis/química , Técnicas Biossensoriais/métodos , Técnicas Biossensoriais/instrumentação , Aptâmeros de Peptídeos/química , Ouro/química , Aptâmeros de Nucleotídeos/química , Antibacterianos/sangue , Antibacterianos/química , Antibacterianos/farmacologia , Fosforilcolina/química , Fosforilcolina/análogos & derivados , Metacrilatos/química
15.
J Mater Chem B ; 12(22): 5360-5376, 2024 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-38700242

RESUMO

Articular cartilage tissue has limited self-repair capabilities, with damage frequently progressing to irreversible degeneration. Engineered tissues constructed through bioprinting and embedded with stem cell aggregates offer promising therapeutic alternatives. Aggregates of bone marrow mesenchymal stromal cells (BMSCs) demonstrate enhanced and more rapid chondrogenic differentiation than isolated cells, thus facilitating cartilage repair. However, it remains a key challenge to precisely control biochemical microenvironments to regulate cellular adhesion and cohesion within bioprinted matrices simultaneously. Herein, this work reports a bioprintable hydrogel matrix with high cellular adhesion and aggregation properties for cartilage repair. The hydrogel comprises an enhanced cell-adhesive gelatin methacrylate and a cell-cohesive chitosan methacrylate (CHMA), both of which are subjected to photo-initiated crosslinking. By precisely adjusting the CHMA content, the mechanical stability and biochemical cues of the hydrogels are finely tuned to promote cellular aggregation, chondrogenic differentiation and cartilage repair implantation. Multi-layer constructs encapsulated with BMSCs, with high cell viability reaching 91.1%, are bioprinted and photo-crosslinked to support chondrogenic differentiation for 21 days. BMSCs rapidly form aggregates and display efficient chondrogenic differentiation both on the hydrogels and within bioprinted constructs, as evidenced by the upregulated expression of Sox9, Aggrecan and Collagen 2a1 genes, along with high protein levels. Transplantation of these BMSC-laden bioprinted hydrogels into cartilaginous defects demonstrates effective hyaline cartilage repair. Overall, this cell-responsive hydrogel scaffold holds immense promise for applications in cartilage tissue engineering.


Assuntos
Bioimpressão , Condrogênese , Hidrogéis , Células-Tronco Mesenquimais , Regeneração , Condrogênese/efeitos dos fármacos , Hidrogéis/química , Hidrogéis/farmacologia , Animais , Células-Tronco Mesenquimais/citologia , Regeneração/efeitos dos fármacos , Cartilagem Articular , Materiais Biomiméticos/química , Materiais Biomiméticos/farmacologia , Diferenciação Celular/efeitos dos fármacos , Engenharia Tecidual , Metacrilatos/química , Sobrevivência Celular/efeitos dos fármacos , Cartilagem/metabolismo , Cartilagem/citologia , Células Cultivadas , Humanos
16.
Langmuir ; 40(23): 11936-11946, 2024 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-38797979

RESUMO

Lipid/copolymer colloidal systems are deemed hybrid materials with unique properties and functionalities. Their hybrid nature leads to complex interfacial phenomena, which have not been fully encoded yet, navigating their properties. Moving toward in-depth knowledge of such systems, a comprehensive investigation of them is imperative. In the present study, hybrid lipid/copolymer structures were fabricated and examined by a gamut of techniques, including dynamic light scattering, fluorescence spectroscopy, cryogenic transmission electron microscopy, microcalorimetry, and high-resolution ultrasound spectroscopy. The biomaterials that were mixed for this purpose at different ratios were 1,2-dioctadecanoyl-sn-glycero-3-phosphocholine and four different linear, statistical (random) amphiphilic copolymers, consisting of oligo(ethylene glycol) methyl ether methacrylate as the hydrophilic comonomer and lauryl methacrylate as the hydrophobic one. The colloidal dispersions were studied for lipid/copolymer interactions regarding their physicochemical, morphological, and biophysical behavior. Their membrane properties and interactions with serum proteins were also studied. The aforementioned techniques confirmed the hybrid nature of the systems and the location of the copolymer in the structure. More importantly, the random architecture of the copolymers, the hydrophobic-to-hydrophilic balance of the nanoplatforms, and the lipid-to-polymer ratio are highlighted as the main design-influencing factors. Elucidating the lipid/copolymer interactions would contribute to the translation of hybrid nanoparticle performance and, thus, their rational design for multiple applications, including drug delivery.


Assuntos
Coloides , Coloides/química , Polímeros/química , Interações Hidrofóbicas e Hidrofílicas , Polietilenoglicóis/química , Metacrilatos/química
17.
Biomater Sci ; 12(12): 3112-3123, 2024 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-38738267

RESUMO

Cardiovascular diseases are a major global cause of morbidity and mortality, and they are often characterized by cardiomyocytes dead that ultimately leads to myocardial ischemia (MI). This condition replaces functional cardiac tissue with fibrotic scar tissue compromising heart function. Injectable systems for the in situ delivery of cells or molecules to assist during tissue repair have emerged as promising approaches for tissue engineering, particularly for myocardial repair. Methacryloyl platelet lysates (PLMA) have been employed for constructing full human-based 3D cell culture matrices and demonstrated potential for xeno-free applications. In this study, we propose using PLMA to produce microparticles (MPs) serving as anchors for cardiac and endothelial cells and ultimately as injectable systems for cardiac tissue repair. The herein reported PLMA MPs were produced by droplet microfluidics and showed great properties for cell attachment. More importantly, it is possible to show the capacity of PLMA MPs to serve as cell microcarriers even in the absence of animal-derived serum supplementation in the culture media.


Assuntos
Materiais Biocompatíveis , Plaquetas , Microgéis , Humanos , Plaquetas/química , Plaquetas/metabolismo , Microgéis/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Miócitos Cardíacos/citologia , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Células Cultivadas , Técnicas de Cultura de Células , Engenharia Tecidual , Metacrilatos/química
18.
J Biomed Mater Res B Appl Biomater ; 112(5): e35412, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38701383

RESUMO

Endodontic therapy, while generally successful, is primarily limited to mature teeth, hence the pressing need to explore regenerative approaches. Gelatin methacryloyl (GelMA) hydrogels have emerged as pivotal biomaterials, promising a bright future for dental pulp regeneration. Despite advancements in tissue engineering and biomaterials, achieving true pulp tissue regeneration remains a formidable task. GelMA stands out for its injectability, rapid gelation, and excellent biocompatibility, serving as the cornerstone of scaffold materials. In the pursuit of dental pulp regeneration, GelMA holds significant potential, facilitating the delivery of stem cells, growth factors, and other vital substances crucial for tissue repair. Presently, in the field of dental pulp regeneration, researchers have been diligently utilizing GelMA hydrogels as engineering scaffolds to transport various effective substances to promote pulp regeneration. However, existing research is relatively scattered and lacks comprehensive reviews and summaries. Therefore, the primary objective of this article is to elucidate the application of GelMA hydrogels as regenerative scaffolds in this field, thereby providing clear direction for future researchers. Additionally, this article provides a comprehensive discussion on the synthesis, characterization, and application of GelMA hydrogels in root canal therapy regeneration. Furthermore, it offers new application strategies and profound insights into future challenges, such as optimizing GelMA formulations to mimic the complex microenvironment of pulp tissue and enhancing its integration with host tissues.


Assuntos
Polpa Dentária , Gelatina , Hidrogéis , Endodontia Regenerativa , Alicerces Teciduais , Hidrogéis/química , Humanos , Alicerces Teciduais/química , Gelatina/química , Polpa Dentária/citologia , Metacrilatos/química , Engenharia Tecidual , Regeneração , Materiais Biocompatíveis/química , Animais
19.
J Nanobiotechnology ; 22(1): 265, 2024 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-38760763

RESUMO

BACKGROUND: Pulp regeneration is a novel approach for the treatment of immature permanent teeth with pulp necrosis. This technique includes the combination of stem cells, scaffolds, and growth factors. Recently, stem cell-derived extracellular vesicles (EVs) have emerged as a new methodology for pulp regeneration. Emerging evidence has proven that preconditioning is an effective scheme to modify EVs for better therapeutic potency. Meanwhile, proper scaffolding is of great significance to protect EVs from rapid clearance and destruction. This investigation aims to fabricate an injectable hydrogel loaded with EVs from pre-differentiated stem cells from human exfoliated deciduous teeth (SHEDs) and examine their effects on pulp regeneration. RESULTS: We successfully employed the odontogenic induction medium (OM) of SHEDs to generate functional EV (OM-EV). The OM-EV at a concentration of 20 µg/mL was demonstrated to promote the proliferation and migration of dental pulp stem cells (DPSCs). The results revealed that OM-EV has a better potential to promote odontogenic differentiation of DPSCs than common EVs (CM-EV) in vitro through Alizarin red phalloidin, alkaline phosphatase staining, and assessment of the expression of odontogenic-related markers. High-throughput sequencing suggests that the superior effects of OM-EV may be attributed to activation of the AMPK/mTOR pathway. Simultaneously, we prepared a photocrosslinkable gelatin methacryloyl (GelMA) to construct an OM-EV-encapsulated hydrogel. The hydrogel exhibited sustained release of OM-EV and good biocompatibility for DPSCs. The released OM-EV from the hydrogel could be internalized by DPSCs, thereby enhancing their survival and migration. In tooth root slices that were subcutaneously transplanted in nude mice, the OM-EV-encapsulated hydrogel was found to facilitate dentinogenesis. After 8 weeks, there was more formation of mineralized tissue, as well as higher levels of dentin sialophosphoprotein (DSPP) and dentin matrix protein-1 (DMP-1). CONCLUSIONS: The effects of EV can be substantially enhanced by preconditioning of SHEDs. The functional EVs from SHEDs combined with GelMA are capable of effectively promoting dentinogenesis through upregulating the odontogenic differentiation of DPSCs, which provides a promising therapeutic approach for pulp regeneration.


Assuntos
Diferenciação Celular , Polpa Dentária , Vesículas Extracelulares , Gelatina , Metacrilatos , Odontogênese , Regeneração , Células-Tronco , Dente Decíduo , Polpa Dentária/citologia , Humanos , Vesículas Extracelulares/química , Gelatina/química , Gelatina/farmacologia , Diferenciação Celular/efeitos dos fármacos , Odontogênese/efeitos dos fármacos , Animais , Células-Tronco/efeitos dos fármacos , Células-Tronco/citologia , Células-Tronco/metabolismo , Regeneração/efeitos dos fármacos , Dente Decíduo/citologia , Metacrilatos/química , Metacrilatos/farmacologia , Camundongos , Proliferação de Células/efeitos dos fármacos , Camundongos Nus , Células Cultivadas , Hidrogéis/química , Hidrogéis/farmacologia , Movimento Celular/efeitos dos fármacos
20.
Dent Mater ; 40(6): 993-1001, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38729779

RESUMO

OBJECTIVES: Acrylamides were shown to significantly improve bonding stability in adhesive restorations, but the reinforcement mechanism has not been fully elucidated. We tested the hypothesis that hydrogen bonding reinforcement of the collagen network (with secondary or tertiary acrylamides), as well as degree of crosslinking of the polymer network (with di- or tri-functional acrylamides), can be two of the factors at play. METHODS: Two-step total etch adhesives comprising UDMA (60 wt%) and 40 wt% of: TAAEA, TMAAEA (secondary, tertiary tri-acrylamides), BAAP, DEBAAP (secondary, tertiary di-acrylamides) or HEMA (mono-methacrylate - control) were formulated. Simulated composite restorations (n = 5) were tested after cyclic mechanical and biological (S. mutans biofilm) challenges. Gap formation before and after aging was assessed with SEM imaging. Micro-tensile bond strength (µTBS, n = 6) was assessed after seven-day incubation in water or S. mutans-containing culture medium. Collagen reinforcement was assessed with hydroxyproline assay (n = 10) and rheology (n = 3). Data were analyzed with one-way/two-way ANOVA/Tukey's test (alpha=5%). RESULTS: Gap formation increased and bond strength decreased for all monomers after biofilm incubation (p < 0.001). Except for DEBAAP, secondary and tertiary di/tri-acrylamides showed lower occlusal gap width values, but no significant differences overall gap length compared to HEMA. µTBS increased for tri-acrylamides compared with HEMA. Samples treated with multi-acrylamides had lower concentration of hydroxyproline (by-product of collagen degradation) (p < 0.001), except for DEBAAP, which showed values close to HEMA (p > 0.05). Dentin shear modulus increased for all acrylamides after 72 h, especially TMAAEA. SIGNIFICANCE: In general, multi-acrylamides promote collagen reinforcement, leading to reduced gap formation, and stabilize the bond strength under physiological conditions.


Assuntos
Acrilamidas , Colágeno , Colagem Dentária , Teste de Materiais , Resistência à Tração , Colágeno/química , Acrilamidas/química , Metacrilatos/química , Resinas Compostas/química , Reologia , Microscopia Eletrônica de Varredura , Ligação de Hidrogênio , Propriedades de Superfície , Análise do Estresse Dentário , Cimentos de Resina/química , Poliuretanos
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