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1.
Sheng Wu Gong Cheng Xue Bao ; 35(9): 1581-1589, 2019 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-31559741

RESUMO

As the main factor leading to foodborne illnesses, foodborne pathogens have been attached great importance by people. The development of simple, rapid, high-sensitivity and low-cost food-borne pathogen detection methods is of great significance in reducing the incidence of foodborne diseases. Biosensor technology is a new micro-analysis technology developed by multi-disciplinary cross-infiltration. It has the characteristics of high sensitivity and fast analysis speed, and is widely used in the detection of food-borne pathogens. This paper introduces the basic principles of biosensors, summarizes the application of common biosensors in the detection of foodborne pathogens, and prospects for future development.


Assuntos
Técnicas Biossensoriais , Doenças Transmitidas por Alimentos , Microbiologia de Alimentos , Humanos
2.
Shokuhin Eiseigaku Zasshi ; 60(4): 88-95, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31474656

RESUMO

Microbial colony counts of concern of food products are one of the most important items in microbiological examinations. The distributions of colony counts per agar plate of food samples are considered to be reflected with microbial cell distributions in food homogenates. However, (i) the probabilistic distributions of the colony counts per agar plate at the dilution of counting and (ii) the relationship between the colony counts per plate and the number of agar plates for food samples have not been intensively studied so far. In this study, therefore, these two points were studied with raw food samples of raw minced beef and chicken and raw milk and microbial culture samples of Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae. Among four major probabilistic distributions, it was found that aerobic plate counts per plate of the foods were well described with negative binomial, Poisson, and normal distributions and that the colony counts per plate of microbial cultures were described well with binomial, Poisson, and normal distributions. The effect of the number of agar plates on the estimation of the mean of colony counts per plate of a sample was then studied with the data randomly resampled from the experimental data. The resampled data showed that with more number of plates the mean of counts fluctuated less and the coefficients of variation of colony counts per plate decreased further, which were coincident to the estimated by the central limit theory. Our study would provide useful information on the characteristics of colony counts per plate of food samples which are routinely examined.


Assuntos
Contagem de Colônia Microbiana , Contaminação de Alimentos/análise , Microbiologia de Alimentos , Ágar , Animais , Bovinos , Escherichia coli/isolamento & purificação , Carne/microbiologia , Leite/microbiologia , Saccharomyces cerevisiae/isolamento & purificação , Staphylococcus aureus/isolamento & purificação
3.
J Agric Food Chem ; 67(33): 9390-9398, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31365249

RESUMO

Various pathogens may coexist in one sample; however, detection methods that rely on traditional selective culture media or immune agents designed specifically for a certain target are unsuitable for multiple targets. It is important to develop a simultaneous and sensitive detection method for multiple pathogens. Here, a multicolor and ultrasensitive enzyme-linked immunosorbent assay (ELISA) platform based on the fluorescence hybridization chain reaction (HCR) was developed. In the assay, multicolor fluorescence concatemers formed as signal amplifiers and signal reporters in the presence of target pathogens. When HCR occurred, Escherichia coli O157:H7, Salmonella serotype Choleraesuis, and Listeria monocytogenes were detected simultaneously with three different fluorescences. Additionally, the limits of detection for E. coli O157:H7, Salmonella Choleraesuis, and L. monocytogenes were 3.4 × 101, 6.4 × 100, and 7.0 × 101 CFU/mL, respectively. The assay achieved ultrasensitive, specific, and simultaneous detection of three pathogens and can be applied to the detection of pathogens in milk samples. Therefore, this multicolor and ultrasensitive ELISA platform has great potential in the application of simultaneous detection of pathogens.


Assuntos
Ensaio de Imunoadsorção Enzimática/métodos , Escherichia coli O157/isolamento & purificação , Listeria monocytogenes/isolamento & purificação , Leite/microbiologia , Salmonella/isolamento & purificação , Animais , Bovinos , Ensaio de Imunoadsorção Enzimática/instrumentação , Fluorescência , Microbiologia de Alimentos , Sensibilidade e Especificidade
4.
Shokuhin Eiseigaku Zasshi ; 60(3): 45-51, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31391410

RESUMO

This study aimed to survey the trend of antimicrobial resistance in Escherichia coli obtained from retail meat. We examined the susceptibilities of 1,115 E. coli isolates obtained from chicken, beef, pork, venison, and wild boar meat from 2011 to 2017 in Tokyo to 14 antimicrobials (ampicillin, cefotaxime (CTX), streptomycin, gentamicin, kanamycin, tetracycline (TC), chloramphenicol, nalidixic acid, ciprofloxacin, sulfamethoxazole-trimethoprim, fosfomycin, amikacin, imipenem, and meropenem). Of all the tested isolates, 18.7% (135/721) isolates from chicken, 77.0% (117/152) from beef, 46.6% (89/187) from pork, 100% (28/28) from venison, and 92.6% (25/27) from wild boar meat were susceptible to all tested antimicrobials. Furthermore, TC resistance was the most common, with rates as high as 56.7% (409/721) and 40.6% (76/187) in the isolates from chicken and pork, respectively. CTX resistance was detected in 4.9% (25/506) of the isolates from domestic chicken and 23.7% (51/215) of the isolates from imported chicken. Moreover, CTX resistance rate in isolates from domestic chicken was significantly lower in 2016 (0.9%, 1/111) and in 2017 (0.8%, 1/121) than in 2012 (10.6%, 17/161). In conclusion, E. coli isolates from retail meat were most commonly resistant to TC, and CTX resistance was higher in E. coli isolates from imported chicken than in E. coli isolates from domestic chicken.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Escherichia coli/efeitos dos fármacos , Microbiologia de Alimentos , Carne/microbiologia , Animais , Bovinos , Galinhas , Cervos , Testes de Sensibilidade Microbiana , Prevalência , Suínos , Tóquio
6.
MMWR Morb Mortal Wkly Rep ; 68(33): 713-717, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31437141

RESUMO

In September 2018, CDC identified Salmonella enterica serotype Newport (Newport) infections that were multidrug resistant (MDR), with decreased susceptibility to azithromycin, a recommended oral treatment agent. Until 2017, decreased susceptibility to azithromycin had occurred in fewer than 0.5% of Salmonella isolates from U.S. residents. This report summarizes the investigation of a multistate MDR Salmonella outbreak conducted by CDC, state and local health departments, and the U.S. Department of Agriculture's Food Safety and Inspection Service. During June 2018-March 2019, 255 cases of infection with the outbreak strain were identified in 32 states; 43% of patients (89 of 206 with information on travel) reported recent travel to Mexico. Infections were linked to consumption of soft cheese obtained in Mexico and beef obtained in the United States. Consumers should avoid eating soft cheese that could be made from unpasteurized milk, regardless of the source of the cheese. When preparing beef, a food thermometer should be used to ensure that appropriate cooking temperatures are reached. When antibiotic treatment is needed for a patient, clinicians should choose antibiotics based on susceptibility testing wherever possible.


Assuntos
Azitromicina/farmacologia , Surtos de Doenças , Farmacorresistência Bacteriana Múltipla , Intoxicação Alimentar por Salmonella/epidemiologia , Salmonella/efeitos dos fármacos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Queijo/microbiologia , Criança , Pré-Escolar , Feminino , Microbiologia de Alimentos , Humanos , Lactente , Masculino , México , Pessoa de Meia-Idade , Carne Vermelha/microbiologia , Salmonella/genética , Intoxicação Alimentar por Salmonella/tratamento farmacológico , Doença Relacionada a Viagens , Estados Unidos/epidemiologia , Adulto Jovem
7.
Bol. latinoam. Caribe plantas med. aromát ; 18(4): 411-424, jul. 2019. tab, ilus
Artigo em Inglês | LILACS | ID: biblio-1008180

RESUMO

Thymol (2-isopropyl-5-methylphenol) is an aromatic monoterpene found in essential oils extracted from plants belonging to the Lamiaceae family, such as Thymus, Ocimum, Origanum, Satureja, Thymbra and Monarda genera. Growth and biofilm formation by Listeria monocytogenes CLIP 74902 were evaluate using three carbon sources in the presence of thymol. Specific growth rate (h-1) values at 37o with glucose, trehalose and cellobiose with the addition of thymol (µg/mL) 0 (control) and 750, were respectively: 0.22, 0.07; 0.14, 0.04; 0.11, 0.04. Lag periods obtained under the same conditions were (h): 8.19, 13.2; 22.5, 27.5; 23.1, 28.1. A marked antibiofilm activity was observed against the exposure with 750 µg/mL of thymol, showing a high percentage of inhibition: glucose (99 %), trehalose (97 %) and cellobiose (98%), compared to the control. The results suggest that thymol could be used to inhibit the growth and production of biofilms by L. monocytogenes in the food industry.


Timol (2-isopropil-5-metilfenol) es un monoterpeno aromático presente en los aceites esenciales extraídos de plantas pertenecientes a la familia Lamiaceae, como los géneros Thymus, Ocimum, Origanum, Satureja, Thymbra y Monarda. El crecimiento y formación de biopelícula por Listeria monocytogenes CLIP 74902 fueron evaluados utilizando tres fuentes de carbono en presencia de timol. La velocidad específica de crecimiento (h-1) a 37o con glucosa, trehalosa y celobiosa con la adición de timol (µg/mL) 0 (control) y 750, fueron respectivamente: 0.22, 0.07; 0.14, 0.04, 0.11, 0,04. Los períodos lag obtenidos en las mismas condiciones fueron (h): 8.19, 13.2; 22.5, 27.5; 23.1, 28.1. Una marcada actividad antibiofilm fue obtenida con 750 µg/mL de timol, mostrando un alto porcentaje de inhibición con glucosa (99%), trehalosa (97%) y celobiosa (98%), respecto al control. Los resultados sugieren que timol podría ser usado para inhibir el crecimiento y producción de biopelículas por L. monocytogenes en la industria alimentaria.


Assuntos
Timol/farmacologia , Biofilmes/efeitos dos fármacos , Listeria monocytogenes/efeitos dos fármacos , Terpenos/farmacologia , Cinética , Biofilmes/crescimento & desenvolvimento , Meio Ambiente , Fermentação , Microbiologia de Alimentos , Listeria monocytogenes/crescimento & desenvolvimento
8.
Onderstepoort J Vet Res ; 86(1): e1-e11, 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31291732

RESUMO

Fasciola spp. are the causative agents of fascioliasis in humans and livestock. Before the development of control and management measures, the geographical distribution of the species and patterns of infection must be considered. Because of difficulties in the phenotypic differentiation and morphometric classification of Fasciola spp., DNA molecular markers have become more useful for fluke differentiation and description of phylogenetic patterns. This study aimed to differentiate and describe the phylogenetic background of Fasciola spp. isolated from cattle slaughtered at three abattoirs in the Mpumalanga and KwaZulu-Natal provinces of South Africa. The cytochrome c oxidase I (COI) - FHCO1 (forward: 5'-TTGGTTTTTTGGGCATCCT-3') and FHCO1 (reverse: 5' -AGGCCACCACCAAATAAAAGA3') - marker was sequenced from 55 Fasciola flukes that were collected from abattoirs in catchment areas of the KwaZulu-Natal and Mpumalanga provinces. Fasciola hepatica was demonstrated to have 100% prevalence in KwaZulu-Natal and Mpumalanga (highveld), respectively, and 76% prevalence in the lowveld (Belfast area) of Mpumalanga. Two animals from the Belfast metapopulation were co-infected with both Fasciola gigantica and F. hepatica. DNA sequence analysis of all the isolates demonstrated a sequence conservation of 0.472, nucleotide diversity of 0.082 and Tajima's D of -1.100; however, it was not statistically significant (p > 0.05). Twenty-two haplotypes were identified, with 18 novel haplotypes being unique to the isolates from South Africa. Within the study samples, 12 haplotypes were isolated to a few individuals, with a haplotype diversity of 0.8957 indicating high genetic diversity. Principal coordinate analysis supported the clustering and distribution of the haplotypes, with 11.38% of the variation being attributed to coordinate 2 and 55.52% to coordinate 1. The distribution of Fasciola spp. has been demonstrated to be related to the distribution of the freshwater intermediate host snails, Lymnaea spp., as well as the relative altitude of the localities in South Africa. Information provided by this study serves as preliminary evidence for further studies on the mapping of the distribution of F. gigantica and F. hepatica in South Africa, which is key in designing control programmes for fascioliasis in humans and livestock.


Assuntos
Doenças dos Bovinos/epidemiologia , Complexo IV da Cadeia de Transporte de Elétrons/genética , Fasciola/isolamento & purificação , Fasciolíase/veterinária , Microbiologia de Alimentos , Matadouros , Animais , Bovinos , Doenças dos Bovinos/parasitologia , DNA de Helmintos/análise , Fasciola/classificação , Fasciola/genética , Fasciolíase/epidemiologia , Marcadores Genéticos , Filogenia , África do Sul/epidemiologia
9.
J Agric Food Chem ; 67(29): 8191-8196, 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31282662

RESUMO

Conversion of free fatty acids into monoacylglycerol gives rise to new structural properties, particularly amphipathic property. Therefore, monoacylglycerols are widely used in pharmaceutical and food industries and are also reported to facilitate better absorption into the human body. A functional fatty acid when transformed into a monoacylglycerol will possibly conserve both the original functionality and amphipathic property. The compound 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was generated from oleic acid by Pseudomonas aeruginosa PR3 and was known to contain antimicrobial activities against a broad range of food-borne and plant pathogenic bacteria. Here, we attempted to convert DOD into its monoacylglycerol form using lipase for producing an amphipathic antibacterial agent. Consequently, the monoacylglycerol of DOD (DOD-MAG) was successfully produced by coincubating DOD, glycerol, and lipase at 30 °C. The maximum conversion yield reached 70% after 12 h of incubation. Antibacterial activity of DOD-MAG was enhanced by 8 times from the original activity of DOD against food-borne bacteria.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Monoglicerídeos/química , Ácidos Oleicos/química , Ácidos Oleicos/farmacologia , Pseudomonas aeruginosa/química , Antibacterianos/metabolismo , Microbiologia de Alimentos , Ácidos Oleicos/metabolismo , Pseudomonas aeruginosa/metabolismo , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento
10.
Rev Inst Med Trop Sao Paulo ; 61: e36, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31340248

RESUMO

During the last years, Brazilian government control programs have detected an increase of Salmonella Heidelberg in poultry slaughterhouses a condition that poses a threat to human health However, the reasons remain unclear. Differences in genetic virulence profiles may be a possible justification. In addition, effective control of Salmonella is related to an efficient epidemiological surveillance system through genotyping techniques. In this context, the aim of this study was the detection of 24 virulence-associated genes in 126 S. Heidelberg isolates. We classified the isolates into 56 different genetic profiles. None of the isolates presented all the virulence genes. The prevalence of these genes was high in all tested samples as the lowest number of genes detected in one isolate was 10/24. The lpfA and csgA (fimbriae), invA and sivH (TTSS), and msgA and tolC (intracellular survival) genes were present in 100% of the isolates analyzed. Genes encoding effector proteins were detected in the majority of SH isolates. No single isolate had the sefA gene. The pefA gene was found in only four isolates. We have also performed a screening of genes associated with iron metabolism: 88.9% of isolates had the iroN geneand 79.4% the sitC gene . Although all the isolates belong to the same serotype, several genotypic profiles were observed. These findings suggest that there is a diversity of S. Heidelberg isolates in poultry products. The fact that a single predominant profile was not found in this study indicates the presence of variable sources of contamination caused by SH. The detection of genetic profiles of Salmonella strains can be used to determine the virulence patterns of SH isolates.


Assuntos
Doenças das Aves Domésticas/microbiologia , Produtos Avícolas/microbiologia , Salmonelose Animal/microbiologia , Salmonella/genética , Salmonella/patogenicidade , Fatores de Virulência/genética , Virulência/genética , Animais , Microbiologia de Alimentos , Genótipo , Reação em Cadeia da Polimerase
11.
Food Chem ; 298: 125080, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260985

RESUMO

Propolis extract was investigated as potential substitute for sorbate in orangeade. Extract was prepared by using aqueous solution of hydroxypropyl-beta-cyclodextrins. Propolis extract was incorporated in non-carbonated orange soft drinks and its antioxidant activity, microbiological stability and color changes were estimated and compared to those of orangeade containing potassium sorbate. l-Ascorbic acid (AsA) degradation at concentrations 0.13 and 1.3% w/w was investigated in the presence of propolis during storage using High Performance Liquid Chromatography-Ion Exclusion Column (HPLC-IEC). The results indicate that the rate of degradation decreased with an increase in ascorbic acid concentration, while addition of propolis affected the degradation rate of samples containing a high AsA concentration. The antifungal effect of propolis extract, potassium sorbate and their combination was assayed. Results showed the inhibition of Aspergillus spp. and B. bruxellensis inhibited in low combined concentrations antimicrobials, while Aspergillus spp. and T. macrosporus were inhibited at 450 mg/g propolis extract.


Assuntos
Bebidas , Conservação de Alimentos/métodos , Conservantes de Alimentos/química , Própole/química , Anti-Infecciosos , Antifúngicos/farmacologia , Antioxidantes/química , Ácido Ascórbico/análise , Ácido Ascórbico/química , Aspergillus/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão/métodos , Cor , Microbiologia de Alimentos , Extratos Vegetais/química , Própole/farmacologia , Ácido Sórbico/farmacologia
12.
Int J Syst Evol Microbiol ; 69(9): 2899-2906, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31274407

RESUMO

A facultative halo-tolerant Aspergillus strain was isolated from olive brine waste, the effluent from the debittering process of table olives. Phenotypic and molecular characteristics showed clearly that the isolate represents a novel species. Based on the source of isolation, the new species has been named Aspergillus olivimuriae. It was found tolerant to high concentrations of NaCl (15 %) or sucrose (60 %) and it exhibits substantial growth under these conditions. Although the new species grew profusely at 37 °C, no growth was observed at 40 °C, conidia en masse were avellaneous on all media. The description of the new species Aspergillus olivimuriae brings the total species of Aspergillus sect. Flavipedes to 15. The type strain of A. olivimuriae sp. nov. is NRRL 66783 (CCF 6208), its whole genome has been deposited as PRJNA498048.


Assuntos
Aspergillus/classificação , Microbiologia de Alimentos , Olea/microbiologia , Filogenia , Sais , Aspergillus/isolamento & purificação , DNA Fúngico/genética , Técnicas de Tipagem Micológica , Pigmentação , Análise de Sequência de DNA , Esporos Fúngicos
13.
Prev Vet Med ; 169: 104703, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31311629

RESUMO

Pig production in Kenya is hampered by seasonal markets. As an alternative outlet for the finished pigs, several value-added meat-processing firms have been established. Sausage, which is produced using casings derived from intestines of pigs, is one form of processed meats. Kenya imports several kgs of natural casings every year; and a recent concern is Swine vesicular disease virus (SVDV), which has never been reported in Kenya, might be introduced via natural casings imported from Italy. To determine conditions (with associated probabilities) that could lead to the introduction of SVDV, a quantitative risk assessment model was developed. Using Monte Carlo simulations at 10,000 iterations, the probability of introducing SVDV was estimated to be 1.9x10-8. Based on the suggested volume of import and mitigations used in the analysis, contaminated casings derived from an estimated 0.003 (Range = 8.1x10-8 - 0.08) infected pigs will be included in the consignment each year. The critical pathway analysis revealed that rigorous surveillance programs in Italy have a potential to dramatically reduce the risk of introducing SVDV into Kenya by this route.


Assuntos
Microbiologia de Alimentos/métodos , Produtos da Carne/virologia , Doença Vesicular Suína/epidemiologia , Doença Vesicular Suína/prevenção & controle , Matadouros , Animais , Enterovirus Humano B , Itália/epidemiologia , Quênia/epidemiologia , Método de Monte Carlo , Medição de Risco , Suínos
14.
Prev Vet Med ; 169: 104708, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31311635

RESUMO

Brucella spp. commonly infect humans in various regions worldwide. Human brucellosis mainly spreads through the consumption of contaminated raw dairy products and meat from domestic livestock (water buffalo, goats, sheep, cattle, pigs and camels). In this regard, the origin and routes of transmission of this bacterium should be carefully determined in order to control the source of infection. This study aimed to evaluate the rate of Brucella spp. contamination of camel milk samples sent for analysis to the national brucellosis laboratory during 2018 in Iran. For this purpose, 96 milk samples from 96 dairy camel herds were randomly collected from two provinces and investigated for the presence of Brucella spp contaminations by both bacterial culture method and polymerase chain reaction (PCR). No clinical manifestation of brucellosis was reported in camels from which milk samples were collected. Using the culture method, three milk samples (3%) originating from two camels of Isfahan province (4%) and one camel from the Semnan province (2%), were contaminated with Brucella abortus. According to PCR analyses, B. abortus gene was detected in 14 (14.5%) milk samples, including 9 and 5 samples from Isfahan (18%) and Semnan (11%) province, respectively. PCR method revealed significant differences (p = 0.02) in the level of contamination with B. abortus between milk samples collected from two regions. These results represent the first report regarding the isolation of B. abortus from raw camel milk in Iran and highlight the importance to screen apparent healthy camels. Therefore, the consumption of raw camel milk may contribute to the spread of human brucellosis in endemic regions.


Assuntos
Brucella abortus/isolamento & purificação , Camelus/microbiologia , Leite/microbiologia , Animais , Brucella abortus/genética , Brucelose/diagnóstico , Brucelose/epidemiologia , Brucelose/veterinária , Primers do DNA , Microbiologia de Alimentos , Irã (Geográfico)/epidemiologia , Reação em Cadeia da Polimerase/veterinária
15.
Toxicol Lett ; 314: 75-81, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31284020

RESUMO

Aflatoxin is a fungal secondary metabolite with high toxicity that is capable of contaminating various types of food crops. It has been identified as a Group 1 human carcinogen by the International Agency for Research on Cancer. Chronic aflatoxin exposure has caused worldwide concern as a matter of public food safety. Peanuts and peanut products are the major sources of aflatoxin exposure. Therefore, some reduction interventions have been developed to minimize contamination throughout the peanut production chain. The purpose of this study is to estimate the efficacy of interventions in reducing the health impact of hepatocellular carcinoma caused by aflatoxin contamination in peanuts. The estimated total Disability-Adjusted Life Years (DALYs) were calculated using FDA-iRISK software. Six aflatoxin reduction strategies were evaluated, including good agricultural practice (GAP), biocontrol, Purdue Improved Crop Storage packaging, basic processing, ozonolysis, and ultraviolet irradiation. The results indicated that basic processing could prevent huge public health loss of 4,079.7-21,833 total DALYs per year. In addition, GAP and biocontrol were both found to be effective strategies in the farm field. Meanwhile, the other three interventions had limited effectiveness in reducing total DALYs. In conclusion, this study could help farmers, processing plants, and government policy makers to alleviate aflatoxin contamination issues in the peanut production chain.


Assuntos
Aflatoxinas/efeitos adversos , Arachis/microbiologia , Carcinoma Hepatocelular/prevenção & controle , Produtos Agrícolas/microbiologia , Exposição Dietética/efeitos adversos , Exposição Dietética/prevenção & controle , Microbiologia de Alimentos/métodos , Doenças Transmitidas por Alimentos/prevenção & controle , Neoplasias Hepáticas/prevenção & controle , Anos de Vida Ajustados por Qualidade de Vida , Agentes de Controle Biológico , Carcinoma Hepatocelular/diagnóstico , Carcinoma Hepatocelular/epidemiologia , Efeitos Psicossociais da Doença , Avaliação da Deficiência , Manipulação de Alimentos/métodos , Armazenamento de Alimentos , Doenças Transmitidas por Alimentos/diagnóstico , Doenças Transmitidas por Alimentos/epidemiologia , Humanos , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/epidemiologia , Ozônio/química , Fatores de Proteção , Medição de Risco , Fatores de Risco , Fatores de Tempo , Raios Ultravioleta
16.
Sci Total Environ ; 691: 252-262, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31323571

RESUMO

Various antimicrobial interventions are applied sequentially in the beef processing industry to reduce microbial load on beef products by using intensive inputs (e.g., chemicals, energy), high strength wastewater, and potentially result in meat discoloration. This study serves as the first analysis to jointly evaluate environmental and economic assessment with its microbial load reduction of proposed antimicrobial systems in the U.S. beef processing industry to identify relatively sustainable systems that minimize environmental and economic impacts while providing microbial safe meat. Specifically, forty potential sequential antimicrobial systems were proposed and evaluated from three perspectives: microbial load reduction, environmental, and economic impacts, by meta-analysis, life cycle assessment, and operational cost analysis orderly. The results show that the antimicrobial systems applying steam pasteurization during the main intervention offer high microbial load reduction (>4.2 log CFU/cm2 reduction from a hypothetical initial contamination at 5.0 log CFU/cm2). Human health impact (31.0 to 65.6%) and ecosystem toxicity (3.6 to 12.5%), eutrophication (11.9 to 15.5%) and global warming (6.4 to 22.2%) are the main contributors to the overall environmental single score among the forty antimicrobial systems. Antimicrobial chemicals (up to 82.8%), wastewater treatment (up to 12.7%), and natural gas (up to 10.7%) are the three major drivers of operational cost for sanitizing 1000 kg hot standard carcass weight (HSCW). Devalued (discolored) meat due to contact with heat from steam pasteurization or hot water wash has a considerable increase in economic ($4.5/1000 HSCW) and environmental (especially at farm stage) impacts. Certain antimicrobial systems (e.g., water wash followed by steam pasteurization) were found to be more promising with satisfactory effectiveness, better environmental and cost performance under uncertainty (1000 Monte Carlo simulations). Results from this study can guide the U.S. beef processing industry to advance sustainability while protecting human health from foodborne illness.


Assuntos
Manipulação de Alimentos/métodos , Microbiologia de Alimentos , Carne Vermelha , Matadouros , Animais , Anti-Infecciosos , Bovinos , Qualidade de Produtos para o Consumidor , Manipulação de Alimentos/estatística & dados numéricos , Doenças Transmitidas por Alimentos , Humanos
17.
Food Chem ; 298: 124980, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260963

RESUMO

Effect of phlorotannins (PT) treatments on the ployhenoloxidase (PPO) activity and quality changes of Pacific white shrimp during a 16-day period of storage in ice were studied. Among seaweeds, Sargassum tenerimum had the highest amount of PT (10.00 mg phloroglucinol/g), PPO inhibitory activity (71.94%) and therefore selected for PT extraction. The shrimp treated with 5% PT (w/v) showed the least melanosis score, pH, TVB-N values and lipid oxidation among all treatments throughout the iced storage. Lower counts of mesophilic and psychrotrophic bacteria (1-2 log CFU/g) were obtained with 5% PT treatment compared to the control at the last day of storage (P < 0.05). Sensory evaluation proved that 5% PT treatment could cause a 4-days increase in the shelf-life of shrimp compared to the control, PT1% and PT2% treatments. Therefore, 5% phlorotannins from S. tenerimum could be used as a safe melanosis inhibitor for the treatment of shrimp.


Assuntos
Armazenamento de Alimentos/métodos , Penaeidae , Floroglucinol/farmacologia , Frutos do Mar , Animais , Bactérias/efeitos dos fármacos , Catecol Oxidase/antagonistas & inibidores , Catecol Oxidase/química , Catecol Oxidase/metabolismo , Microbiologia de Alimentos , Qualidade dos Alimentos , Gelo , Melanose , Oxirredução , Penaeidae/efeitos dos fármacos , Penaeidae/microbiologia , Sargassum/química , Frutos do Mar/microbiologia
18.
Int J Syst Evol Microbiol ; 69(10): 3178-3190, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31339481

RESUMO

Fourteen Gram-stain-positive bacterial strains were isolated from Chinese traditional pickle and yogurt. The strains were characterised using a polyphasic taxonomic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, determination of DNA G+C content, determination of average nucleotide identity, fatty acid methyl ester analysis and an analysis of phenotypic features. The data demonstrated that the fourteen strains represented nine novel species belonging to the genus Lactobacillus, strains 54-2T, 54-5T, 33-7T, 116-2T, 184-8T, 204-8T, 8-1(1)T, 256-3T and M1575T were designated as the type strains. Strain 54-2T was phylogenetically related to the type strains of Lactobacillus composti and Lactobacillus floricola, having 96.5 and 91.6 % 16S rRNA gene sequence similarities, less than 74.6 % pheS gene sequence similarities, less than 81.6 % rpoA gene sequence similarities and less than 72.5 % ANI values. Strain 54-5T was phylogenetically related to the type strains of Lactobacillus dextrinicus and Lactobacillus concavus, exhibiting 99.1 and 97.3 % 16S rRNA gene sequence similarities, less than 83.1 % pheS gene sequence similarities, less than 93.1 % rpoA gene sequence similarities and less than 79.9 % ANI values. Strains 33-7T, 116-2T, 184-8T, 204-8T, 8-1(1)T, 256-3T and M1575T were phylogenetically related to the type strains of Lactobacillus tucceti, Lactobacillus nodensis, Lactobacillus insicii, Lactobacillus allii, Lactobacillus metriopterae, Lactobacillus terrae, Lactobacillus versmoldensis and Lactobacillus furfuricola, sharing 95.6-100 % 16S rRNA gene sequence similarities, less than 91.6 % pheS gene sequence similarities, less than 98.2 % rpoA gene sequence similarities and less than 89.4 % ANI values. Based upon the data of polyphasic characterisation obtained in the present study, nine novel species, Lactobacillus yilanensis sp. nov., Lactobacillus bayanensis sp. nov., Lactobacillus keshanensis sp. nov., Lactobacillus kedongensis sp. nov., Lactobacillus baiquanensis sp. nov., Lactobacillus jidongensis sp. nov., Lactobacillus hulinensis sp. nov., Lactobacillus mishanensis sp. nov. and Lactobacillus zhongbaensis sp. nov., are proposed and the type strains are 54-2T (=NCIMB 15154T=CCM 8896T=KCTC 21120T=LMG 31058T), 54-5T (=NCIMB 15151T=CCM 8894T), 33-7T (=NCIMB 15153T=CCM 8936T=KCTC 21118T=LMG 31166T), 116-2T (=NCIMB 15158T=CCM 8899T=KCTC 21124T=LMG 31051T), 184-8T (=NCIMB 15152T=CCM 8895T=KCTC 21131T=LMG 31050T), 204-8T (=NCIMB 15159T=CCM 8900T=KCTC 21133T=LMG 31054T), 8-1(1)T (=NCIMB 15156T=CCM 8898T=KCTC 21115T=LMG 31047T), 256-3T (=NCIMB 15160T=CCM 8901T=LMG 31048T) and M1575T (=NCIMB 15149T=CCM 8892T=LMG 31045T), respectively.


Assuntos
Alimentos Fermentados/microbiologia , Microbiologia de Alimentos , Lactobacillus/classificação , Filogenia , Iogurte/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Genes Bacterianos , Lactobacillus/isolamento & purificação , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
19.
Int J Syst Evol Microbiol ; 69(10): 3191-3201, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31339483

RESUMO

Five novel lactic acid bacterial strains, isolated from Chinese traditional pickle juice, were characterised by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, determination of DNA G+C content, average nucleotide identity (ANI) analysis, in silico DNA-DNA hybridisation (isDDH), fatty acid methyl ester (FAME) analysis and an analysis of phenotypic features. Strains 241-2-2T, 63-4T and 190-7T were closely related to the type strains of Enterococcus devriesei, Enterococcus viikkiensis, Enterococcus pseudoavium, Enterococcus xiangfangensis, Enterococcus avium, Enterococcus malodoratus, Enterococcus raffinosus and Enterococcus gilvus, having 99.1-99.9 % 16S rRNA gene sequence similarities. Strain 94-2T was distantly related to the type strains of Enterococcus phoeniculicola, Enterococcus rivorum and Enterococcus faecalis, having 95.2-96.1 % 16S rRNA gene sequence similarities. Strain 85-4T was distantly related to the type strains of Enterococcus casseliflavus, Enterococcus gallinarum, Enterococcus dispar, Enterococcus canintestini, Enterococcus saigonensis, Enterococcus diestrammenae, Enterococcus asini, Enterococcus cecorum and Enterococcus columbae, having 95.7-97.8 % 16S rRNA gene sequence similarities. Less than 91.5 % ANI and 45.3 % isDDH values between strains 241-2-2T, 63-4T, 190-7T, 94-2T, 85-4T and type strains of phylogenetically related species showed that they represent five new species within the genus Enterococcus. Based upon the data of polyphasic characterisation obtained in the present study, five novel species, Enterococcus pingfangensis sp. nov., Enterococcus dongliensis sp. nov., Enterococcus hulanensis sp. nov., Enterococcus nangangensis sp. nov. and Enterococcus songbeiensis sp. nov., are proposed and the type strains are 241-2-2T (=NCIMB 15185T=CCM 8921T=LMG 31181T), 63-4T (=NCIMB 15178T=CCM 8922T), 190-7T (=NCIMB 15200T=CCM 8949T), 94-2T (=NCIMB 15180T=CCM 8920T=LMG 31180T) and 85-4T (=NCIMB 15179T=CCM 8923T=LMG 31183T), respectively.


Assuntos
Enterococcus/classificação , Alimentos Fermentados/microbiologia , Microbiologia de Alimentos , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Enterococcus/isolamento & purificação , Ácidos Graxos/química , Genes Bacterianos , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
20.
Int J Syst Evol Microbiol ; 69(10): 3237-3247, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31361212

RESUMO

Seven novel lactic acid bacterial strains, isolated from traditional Chinese pickle, were characterized using a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, determination of DNA G+C content, determination of average nucleotide identity (ANI), in silico DNA-DNA hybridization (isDDH), fatty acid methyl ester (FAME) analysis and an analysis of phenotypic features. Strains 382-1T, 116-1AT, 381-7T, 203-3T, 218-3T and 398-2T were phylogenetically related to the type strains of Lactobacillus plantarumsubsp. plantarum, Lactobacillus plantarumsubsp. argentoratensis, Lactobacillus pentosus, Lactobacillus paraplantarum, Lactobacillus fabifermentans, Lactobacillus herbarum, Lactobacillus mudanjiangensis, Lactobacillus xiangfangensis, Lactobacillus plajomi and Lactobacillus modestisalitolerans, having 97.1-99.9 % 16S rRNA gene sequence similarities, less than 89.9 % pheS gene sequence similarities, less than 98.0 % rpoA gene sequence similarities, less than 91.2 % ANI values and less than 43.3 % isDDH values. Strain 778-3T was phylogenetically related to the type strains of Lactobacillus hokkaidonensis, Lactobacillus wasatchensis, Lactobacillus oligofermentans, Lactobacillus nenjiangensis, Lactobacillus vaccinostercus and Lactobacillus suebicus, exhibiting 97.0-99.4 % 16S rRNA gene sequence similarities, 78.2-82.1 % pheS gene sequence similarities, 80.0-91.5 % rpoA gene sequence similarities, less than 78.6 % ANI values and less than 22.9 % isDDH values. Based upon the data of polyphasic characterization obtained in the present study, seven novel species, Lactobacillus pingfangensis sp. nov., Lactobacillus daoliensis sp. nov., Lactobacillus nangangensis sp. nov., Lactobacillus daowaiensis sp. nov., Lactobacillus dongliensis sp. nov., Lactobacillus songbeiensis sp. nov. and Lactobacillus kaifaensis sp. nov., are proposed and the type strains are 382-1T (=NCIMB 15187T=CCM 8935T=LMG 31176T), 116-1AT (=NCIMB 15181T=CCM 8934T=LMG 31171T), 381-7T (=NCIMB 15186T=CCM 8930T), 203-3T (=NCIMB 15183T=CCM 8933T=LMG 31172T), 218-3T (=NCIMB 15184T=CCM 8932T=LMG 31173T), 398-2T (=NCIMB 15189T=CCM 8931T=LMG 31174T) and 778-3T (=NCIMB 15191T=CCM 8929T=LMG 31177T), respectively.


Assuntos
Alimentos Fermentados/microbiologia , Microbiologia de Alimentos , Lactobacillus/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Genes Bacterianos , Lactobacillus/isolamento & purificação , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
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