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1.
Sud Med Ekspert ; 63(1): 15-19, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32040082

RESUMO

Aim of this study is to determine the influence of the basic conditions of the traumatic interaction of cutting and chopping objects with 'barrier' tissues of the scalp on the formation of morphological signs of damage. It was suggested to perform the comparative analysis of the morphological signs of incised and chopped scalp tissue wounds obtained by changing one given parameter in experimental damage modeling. The influence of the basic conditions of the traumatic interaction of cutting and chopping objects with the 'barrier' tissues of the scalp on the formation of morphological signs of the resulting damage was determined. The effectiveness of the proposed comparative analysis of morphological signs of incised and chopped lesions was confirmed.


Assuntos
Traumatismos Craniocerebrais , Ferimentos Perfurantes , Medicina Legal , Humanos , Microscopia
2.
Mikrobiyol Bul ; 54(1): 135-143, 2020 Jan.
Artigo em Turco | MEDLINE | ID: mdl-32050884

RESUMO

Sexually transmitted infections (STIs) are important as a public health problem all over the world. There are some difficulties in prevention and control programs of STIs due to clinical and laboratory diagnostic problems.The most common STIs are Chlamydia trachomatis infections, trichomoniasis and gonorrhea. The study aimed to investigate the direct microscopic examination, culture and polymerase chain reaction (PCR) tests in the diagnosis of Trichomonas vaginalis infection; to determine other microbiological agents that may cause vaginal discharge and to evaluate the various social variables in women with vaginal discharge admitted to the outpatient clinic of Obstetrics and Gynecology in Akdeniz University Hospital. Two hundred and fifteen patients were enrolled in the study. The socio-demographic features of the patients were recorded. Vaginal/endocervical swab specimens taken from patients were evaluated by microscopic examination. Swab specimens were inoculated into blood agar, MacConkey agar and chocolate agar for bacterial culture. Modified Trichosel broth with 5% horse blood (Becton Dickinson, USA) was used for Trichomonas spp. culture. The presence of C.trachomatis, Neisseria gonorrhoeae, and T.vaginalis in swab samples were investigated by multiplex PCR assay (BD Max CT/GC/TV, Becton Dickinson, USA). At least one pathogen was detected among 65 (30.3%) samples. T.vaginalis was detected by microscopic examination and PCR in four of 215 (1.9%) patients. Existence of yeast morphology was observed in 21 (9.8%) specimens by microscopic examination. Twenty four (11.2%) patients were diagnosed as bacterial vaginosis microscopically according to Nugent score system. Candida species grew in 32 (14.9%) and Streptococcus agalactiae grew in 2 (0.9%) of the specimens. C.trachomatis was detected in 2 (0.9%) samples and N.gonorrhoeae in 1 (0.5%) sample by PCR. In this study, 95.3% of the patients were married and 96.7% had only one sexual partner in the mean time. The rate of detection of pathogens were statistically higher in women who have had two or more pregnancies (p<0.05). In our study, T.vaginalis together with N.gonorrhoeae and C.trachomatis were investigated by PCR method in women with vaginal discharge. The use of multiplex PCR test allowed simultaneous investigation of multiple pathogens in the patient samples.


Assuntos
Infecções por Chlamydia , Técnicas e Procedimentos Diagnósticos , Gonorreia , Tricomoníase , Vaginite por Trichomonas , Técnicas de Cultura de Células , Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis/genética , Técnicas e Procedimentos Diagnósticos/normas , Feminino , Gonorreia/diagnóstico , Humanos , Microscopia/normas , Reação em Cadeia da Polimerase Multiplex , Neisseria gonorrhoeae/genética , Gravidez , Tricomoníase/diagnóstico , Tricomoníase/parasitologia , Vaginite por Trichomonas/diagnóstico , Trichomonas vaginalis/genética
3.
Eur. j. anat ; 24(1): 1-7, ene. 2020. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-186059

RESUMO

The aim of this study is to demonstrate that the denervation of the pancreas may affect the enteric neuronal plexus, which controls both the endocrine and exocrine parts of the pancreas. By using the light microscope, the histological changes of the islets of Langerhans and the pancreatic acini in the rat pancreas were studied two and three weeks after sympathectomy and truncal vagotomy. More-over, measurements of the changes infasting blood glucose levels and glucose tolerance tests in the control and experimental animals were recorded. Atrophic changes and degeneration of the pancreatic acinar cells and islets of Langerhans cells were observed after both sympathectomy and vagotomy. Biochemical measurements of fasting blood, and the glucose tolerance tests after sympathectomy and vagotomy were increased significantly, which is consistent with the histological results. The results of this study explain that the exocrine and endocrine parts of the pancreas are dependent on both sympathetic and parasympathetic innervation via the enteric plexuses of the rat pancreas. These results establish a firm correlation between the autonomic innervation and the enteric plexus, which controls the function of the endocrine and exocrine parts of the pancreas


No disponible


Assuntos
Animais , Ratos , Pâncreas/anatomia & histologia , Pâncreas/diagnóstico por imagem , Microscopia/métodos , Microscopia/veterinária , Denervação/veterinária , Simpatectomia/métodos , Simpatectomia/veterinária , Vagotomia Troncular/métodos , Vagotomia Troncular/veterinária , Ilhotas Pancreáticas/anatomia & histologia
4.
Forensic Sci Int ; 307: 110104, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31918163

RESUMO

When adult flies are collected at indoor crime scenes, the question of their origin arises, i.e., whether they originated from the outdoors, coming in to oviposit on the body, or if they developed through the larval and pupal stages from eggs laid on the body. This is particularly important if no empty puparia were collected, potentially because they were overlooked, with a consequent impact on the accuracy of the minimum post-mortem interval estimation. This study used two methods to determine if flies sampled in various experimental conditions and at an actual crime scene were wild flies attracted to the body or had developed on the body, i.e., through the assessment of: (1) wing fray and; (2) sex ratio. A template was created to measure the amount of wing fray damage quantitatively in a simple, rapid and efficient manner. The experiments demonstrated that by combining the two methods it is possible to establish the origin of a population of adult flies at a crime scene.


Assuntos
Dípteros/anatomia & histologia , Asas de Animais/anatomia & histologia , Animais , Comportamento Alimentar , Feminino , Processamento de Imagem Assistida por Computador , Larva , Masculino , Microscopia , Mudanças Depois da Morte , Pupa , Razão de Masculinidade
6.
BMC Bioinformatics ; 21(1): 8, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31914944

RESUMO

BACKGROUND: Cell nuclei segmentation is a fundamental task in microscopy image analysis, based on which multiple biological related analysis can be performed. Although deep learning (DL) based techniques have achieved state-of-the-art performances in image segmentation tasks, these methods are usually complex and require support of powerful computing resources. In addition, it is impractical to allocate advanced computing resources to each dark- or bright-field microscopy, which is widely employed in vast clinical institutions, considering the cost of medical exams. Thus, it is essential to develop accurate DL based segmentation algorithms working with resources-constraint computing. RESULTS: An enhanced, light-weighted U-Net (called U-Net+) with modified encoded branch is proposed to potentially work with low-resources computing. Through strictly controlled experiments, the average IOU and precision of U-Net+ predictions are confirmed to outperform other prevalent competing methods with 1.0% to 3.0% gain on the first stage test set of 2018 Kaggle Data Science Bowl cell nuclei segmentation contest with shorter inference time. CONCLUSIONS: Our results preliminarily demonstrate the potential of proposed U-Net+ in correctly spotting microscopy cell nuclei with resources-constraint computing.


Assuntos
Núcleo Celular/patologia , Microscopia , Aprendizado Profundo , Humanos , Processamento de Imagem Assistida por Computador/métodos
7.
BMC Med ; 18(1): 9, 2020 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-31987052

RESUMO

BACKGROUND: In order to improve malaria burden estimates in low transmission settings, more sensitive tools and efficient sampling strategies are required. This study evaluated the use of serological measures from repeated health facility-based cross-sectional surveys to investigate Plasmodium falciparum and Plasmodium vivax transmission dynamics in an area nearing elimination in Indonesia. METHODS: Quarterly surveys were conducted in eight public health facilities in Kulon Progo District, Indonesia, from May 2017 to April 2018. Demographic data were collected from all clinic patients and their companions, with household coordinates collected using participatory mapping methods. In addition to standard microscopy tests, bead-based serological assays were performed on finger-prick bloodspot samples from 9453 people. Seroconversion rates (SCR, i.e. the proportion of people in the population who are expected to seroconvert per year) were estimated by fitting a simple reversible catalytic model to seroprevalence data. Mixed effects logistic regression was used to examine factors associated with malaria exposure, and spatial analysis was performed to identify areas with clustering of high antibody responses. RESULTS: Parasite prevalence by microscopy was extremely low (0.06% (95% confidence interval 0.03-0.14, n = 6) and 0 for P. vivax and P. falciparum, respectively). However, spatial analysis of P. vivax antibody responses identified high-risk areas that were subsequently the site of a P. vivax outbreak in August 2017 (62 cases detected through passive and reactive detection systems). These areas overlapped with P. falciparum high-risk areas and were detected in each survey. General low transmission was confirmed by the SCR estimated from a pool of the four surveys in people aged 15 years old and under (0.020 (95% confidence interval 0.017-0.024) and 0.005 (95% confidence interval 0.003-0.008) for P. vivax and P. falciparum, respectively). The SCR estimates in those over 15 years old were 0.066 (95% confidence interval 0.041-0.105) and 0.032 (95% confidence interval 0.015-0.069) for P. vivax and P. falciparum, respectively. CONCLUSIONS: These findings demonstrate the potential use of health facility-based serological surveillance to better identify and target areas still receptive to malaria in an elimination setting. Further implementation research is needed to enable integration of these methods with existing surveillance systems.


Assuntos
Surtos de Doenças , Malária Falciparum/epidemiologia , Malária Vivax/epidemiologia , Estudos Soroepidemiológicos , Adulto , Análise por Conglomerados , Estudos Transversais , Testes Diagnósticos de Rotina , Feminino , Instalações de Saúde , Humanos , Indonésia/epidemiologia , Modelos Logísticos , Malária/epidemiologia , Masculino , Microscopia , Pessoa de Meia-Idade , Plasmodium falciparum/imunologia , Plasmodium vivax/imunologia , Prevalência , Análise Espacial
8.
Braz Oral Res ; 33: e121, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31939500

RESUMO

The objective of the study was to analyze the surface area (SA) of the wear caused by simulated chewing on human enamel and opposing restorative material, namely: composite resin (CR), porcelain fused to metal (PFM), lithium disilicate (LD), or monolithic zirconia (MZr). Forty-eight premolars were selected as enamel specimens and divided randomly into 4 groups (n = 48; n =12) used as antagonists in chewing simulation (250,000 loading cycles) against one of the four selected test materials. Enamel and material specimens were scanned and evaluated under digital microscope, and wear SA (mm2) were recorded. Descriptive statistics, paired t-test, one-way ANOVA, and post-hoc Tukey-HSD tests were used for statistics (p < 0.05). The smallest and largest SA were exhibited by enamel against LD (0.80 mm2) and PFM (1.74 mm2), respectively. PFM (3.48 mm2) showed the largest SA and CR (2.28 mm2) showed the smallest SA. Paired t-test for SA values showed significant difference (p < 0.05) in all wear comparisons between materials and enamel antagonists. The wear of materials were greater than that of their respective enamel antagonists (p < 0.05). One-way ANOVA of the logarithmic means of wear SA revealed significant differences (P<0.05). Post-hoc Tukey test revealed significance for PFM (p < 0.05) with other materials. Wear of all test materials was greater compared to the wear of enamel antagonists. PFM and LD caused the largest and the smallest enamel wear, respectively. CR, LD, and MZr are more resistant than PFM to wear after simulated chewing against enamel.


Assuntos
Resinas Compostas/química , Esmalte Dentário/química , Porcelana Dentária/química , Mastigação , Ligas Metalo-Cerâmicas/química , Desgaste dos Dentes/etiologia , Zircônio/química , Adolescente , Adulto , Análise de Variância , Polimento Dentário/métodos , Humanos , Processamento de Imagem Assistida por Computador , Teste de Materiais , Microscopia/instrumentação , Valores de Referência , Estatísticas não Paramétricas , Propriedades de Superfície , Adulto Jovem
9.
Science ; 367(6476): 368, 2020 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-31974236
12.
J Forensic Sci ; 65(1): 84-89, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31454431

RESUMO

Impressions of friction ridges left on a surface are important evidence for identifying an individual and can be used to confirm his or her presence at the scene of an incident. Factors influencing the durability of fingerprints include the physiological characteristics and cleanliness of the individual, environmental factors, and time. The aim of the study was to determine the effect of atmospheric factors, gender, hand cleanliness and time on the width of friction ridge impressions and the amount of material forming the print. The research shows that factors such as gender, ambient conditions, and hand cleanliness affect the width of the ridge impressions and the quantity of material forming the print. The passage of time significantly reduces both the width of the ridge impressions and the quantity of the material forming the print.


Assuntos
Dermatoglifia , Higiene das Mãos , Manejo de Espécimes , Adulto , Escuridão , Exposição Ambiental , Feminino , Humanos , Luz , Masculino , Microscopia , Fatores Sexuais , Creme para a Pele , Temperatura Ambiente , Fatores de Tempo , Adulto Jovem
13.
Ophthalmic Res ; 63(1): 34-40, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31352453

RESUMO

AIM: To report the outcomes of ab externo surgery using a surgical microscope, wide-angle viewing system, and chandelier endoilluminator (microscope-assisted ab externo surgery) for rhegmatogenous retinal detachment (RRD). METHODS: This was a retrospective study. Consecutive charts of patients with RRD who underwent microscope-assisted ab externo surgery were analyzed. The following demographic parameters were analyzed: age (years), gender (male/female), and eye (right/left). Clinical parameters were axial length (AL) measured in millimeters (mm), preoperative best-corrected visual acuity (BCVA) measured in logarithm of minimum angle of resolution (logMAR), intraocular pressure (IOP), and lens status (phakic/pseudophakic). The parameters of RRD were number and type of retinal breaks, location of retinal breaks, extent of retinal detachment (RD) (number of detached quadrants), and macular detachment (MD), as well as retinal breaks not detected preoperatively. Use of cryopexy, circumferential or segmental scleral buckle, drainage of subretinal fluid, injection of air or gas, and duration of surgery were recorded. The postoperative parameters analyzed were BCVA, IOP and recurrence of RD and postoperative complications. Follow-up was established at 3 months. RESULTS: A total of 213 eyes (97 right, 116 left) of 205 patients (114 males, 91 females) affected by primary RRD were included. Fifty-two eyes (24.4%) were affected by high myopia (AL >26.5 mm), and 160 patients (75.1%) were affected by RRD caused by a single retinal break and involving only one quadrant. The superior quadrant was the most frequently involved (49.3%). Forty-two eyes (19.7%) were affected by MD. In 13 eyes (11.3%), retinal breaks were not detected preoperatively. The duration of surgery was 75.5 ± 42 min. No significant BCVA changes were observed in the whole group, whereas a significant improvement of BCVA from the baseline (2.83 ± 0.87 logMAR) to each time point of follow-up was observed in the subgroup of patients affected by MD. Six eyes (2.8%) developed a recurrent RD, secondary to proliferative vitreoretinopathy (3 eyes) and secondary to a new retinal break (3 eyes). Two eyes developed a persistent vitreous hemorrhage, and one eye developed a macular hole after 1 week. PPV was performed for both. CONCLUSION: Microscope-assisted ab externo surgery is effective and safe, it reduces discomfort, it allows the surgeon to work with both hands free, and provides an adequate visualization of each step being performed.


Assuntos
Crioterapia/métodos , Tamponamento Interno/métodos , Procedimentos Cirúrgicos Oftalmológicos/métodos , Descolamento Retiniano/cirurgia , Adulto , Idoso , Feminino , Humanos , Masculino , Microscopia/métodos , Pessoa de Meia-Idade , Perfurações Retinianas/cirurgia , Estudos Retrospectivos , Acuidade Visual
14.
Ophthalmologe ; 117(2): 158-165, 2020 Feb.
Artigo em Alemão | MEDLINE | ID: mdl-31728615

RESUMO

The complete integration of spectral domain optical coherence tomography (SD-OCT) into an operating microscope now enables targeted, high-resolution imaging-guided vitreoretinal surgery. This provides real-time visualization of retinal layers, vitreous body and instrument-tissue interactions, which can be used for intraoperative decision making. Compared to conventional surgical microscopes, intraoperative OCT enables a greatly enhanced precision of vitreoretinal surgical maneuvers and is an essential prerequisite for the implementation of real-time guided surgical techniques at the micrometer level.


Assuntos
Tomografia de Coerência Óptica , Cirurgia Vitreorretiniana , Microscopia , Retina , Corpo Vítreo
15.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117386, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31336320

RESUMO

Non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups such as O26, O45, O103, O111, O121 and O145 often cause illness to people in the United States and the conventional identification of these "Big-Six" are complex. The label-free hyperspectral microscope imaging (HMI) method, which provides spectral "fingerprints" information of bacterial cells, was employed to classify serogroups at the cellular level. In spectral analysis, principal component analysis (PCA) method and stacked auto-encoder (SAE) method were conducted to extract principal spectral features for classification task. Based on these features, multiple classifiers including linear discriminant analysis (LDA), support vector machine (SVM) and soft-max regression (SR) methods were evaluated. Different sizes of datasets were also tested in search for the suitable classification models. Among the results, SAE-based classification models performed better than PCA-based models, achieving classification accuracy of SAE-LDA (93.5%), SAE-SVM (94.9%) and SAE-SR (94.6%), respectively. In contrast, classification results of PCA-based methods such as PCA-LDA, PCA-SVM and PCA-SR were only 75.5%, 85.7% and 77.1%, respectively. The results also suggested the increasing number of training samples have positive effects on classification models. Taking advantage of increasing dataset, the SAE-SR classification model finally performed better than others with average accuracy of 94.9% in classifying STEC serogroups. Specifically, O103 serogroup was classified with the highest accuracy of 97.4%, followed by O111 (96.5%), O26 (95.3%), O121 (95%), O145 (92.9%) and O45 (92.4%), respectively. Thus, the HMI technology coupled with SAE-SR classification model has the potential for "Big-Six" identification.


Assuntos
Técnicas de Tipagem Bacteriana/métodos , Aprendizado Profundo , Processamento de Imagem Assistida por Computador/métodos , Microscopia/métodos , Escherichia coli Shiga Toxigênica , Algoritmos , Microbiologia de Alimentos , Doenças Transmitidas por Alimentos/microbiologia , Humanos , Imagem Óptica/métodos , Análise de Componente Principal , Escherichia coli Shiga Toxigênica/química , Escherichia coli Shiga Toxigênica/classificação
16.
Chemosphere ; 238: 124564, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31472348

RESUMO

Microplastics pose a worldwide risk for the environment. Microplastic fibers, which are released during the household washing of synthetic fabrics, are a substantial percentage of microplastics in rivers and in oceans. A novel quantification and simultaneous identification of fiber polymers via Micro-FTIR (Fourier Transform Infrared Spectroscopy) was developed. Washing simulations with commercially available household products were performed and effluents were filtered either on GF/F filters (0.7 µm) or on Anodisc filter (0.2 µm), to gather even the smallest fibers. Furthermore, a novel purification procedure of effluents was developed. Subsequently, filters were analyzed also with the scanning electronic microscope (SEM) to confirm the width and length of fibers. This novel method is robust and replicable and it allows better quantification of fibers released and identification of fiber polymers with optimal matches (averagely 80%).


Assuntos
Monitoramento Ambiental/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Poluentes Químicos da Água/análise , Filtração , Produtos Domésticos/análise , Microscopia , Têxteis/análise
17.
Nat Commun ; 10(1): 5504, 2019 12 03.
Artigo em Inglês | MEDLINE | ID: mdl-31796741

RESUMO

3D histology, slice-based connectivity atlases, and diffusion MRI are common techniques to map brain wiring. While there are many modality-specific tools to process these data, there is a lack of integration across modalities. We develop an automated resource that combines histologically cleared volumes with connectivity atlases and MRI, enabling the analysis of histological features across multiple fiber tracts and networks, and their correlation with in-vivo biomarkers. We apply our pipeline in a murine stroke model, demonstrating not only strong correspondence between MRI abnormalities and CLARITY-tissue staining, but also uncovering acute cellular effects in areas connected to the ischemic core. We provide improved maps of connectivity by quantifying projection terminals from CLARITY viral injections, and integrate diffusion MRI with CLARITY viral tracing to compare connectivity maps across scales. Finally, we demonstrate tract-level histological changes of stroke through this multimodal integration. This resource can propel investigations of network alterations underlying neurological disorders.


Assuntos
Conectoma , Processamento de Imagem Assistida por Computador , Imagem por Ressonância Magnética , Microscopia , Animais , Automação , Axônios/metabolismo , Modelos Animais de Doenças , Infarto da Artéria Cerebral Média/complicações , Infarto da Artéria Cerebral Média/diagnóstico por imagem , Infarto da Artéria Cerebral Média/patologia , Camundongos Endogâmicos C57BL , Reprodutibilidade dos Testes , Software , Acidente Vascular Cerebral/complicações , Acidente Vascular Cerebral/diagnóstico por imagem , Acidente Vascular Cerebral/patologia
18.
PLoS Pathog ; 15(12): e1008209, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31790506

RESUMO

The processes of cell attachment and membrane fusion of Herpes Simplex Virus 1 involve many different envelope glycoproteins. Viral proteins gC and gD bind to cellular receptors. Upon binding, gD activates the gH/gL complex which in turn activates gB to trigger membrane fusion. Thus, these proteins must be located at the point of contact between cellular and viral envelopes to interact and allow fusion. Using super-resolution microscopy, we show that gB, gH/gL and most of gC are distributed evenly round purified virions. In contrast, gD localizes essentially as clusters which are distinct from gB and gH/gL. Upon cell binding, we observe that all glycoproteins, including gD, have a similar ring-like pattern, but the diameter of these rings was significantly smaller than those observed on cell-free viruses. We also observe that contrary to cell-free particles, gD mostly colocalizes with other glycoproteins on cell-bound particles. The differing patterns of localization of gD between cell-free and cell-bound viruses indicates that gD can be reorganized on the viral envelope following either a possible maturation of the viral particle or its adsorption to the cell. This redistribution of glycoproteins upon cell attachment could contribute to initiate the cascade of activations leading to membrane fusion.


Assuntos
Herpesvirus Humano 1/metabolismo , Proteínas do Envelope Viral/metabolismo , Vírion/metabolismo , Linhagem Celular , Glicoproteínas/metabolismo , Glicoproteínas/ultraestrutura , Herpesvirus Humano 1/ultraestrutura , Humanos , Microscopia/métodos , Proteínas do Envelope Viral/ultraestrutura , Vírion/ultraestrutura , Ligação Viral , Internalização do Vírus
19.
Actas dermo-sifiliogr. (Ed. impr.) ; 110(10): 841-849, dic. 2019. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-185606

RESUMO

Introducción: Desde el año 2000 se ha descrito un aumento progresivo de los casos de sífilis en diferentes ciudades europeas, sobre todo en hombres que tienen sexo con hombres (HSH). Las manifestaciones clínicas de la sífilis pueden estar influidas por la coinfección del VIH o por el antecedente de sífilis. El objetivo del presente estudio es describir las características epidemiológicas y clínicas de los casos de sífilis precoz diagnosticados en Barcelona. Métodos: Estudio retrospectivo de casos de sífilis precoz diagnosticados en Barcelona (2003-2013). Se revisaron las historias clínicas y los datos epidemiológicos y clínicos fueron recogidos de forma estructurada. Las variables se compararon según el estatus serológico del VIH y el antecedente de sífilis. Resultados: Se diagnosticaron 1.702 casos de sífilis precoz (37% primaria, 48% secundaria y 14% latente precoz), 93% de ellos en HSH. Entre ellos, el 40% presentaba una coinfección por VIH. Los casos de sífilis en pacientes VIH positivos estaban asociados con un episodio previo de sífilis (aOR, 5,2 [IC del 95%, 3,32-8,24]) y con haber tenido sexo anal desprotegido (aOR, 1,75 [IC del 95%, 1,17-2,63]). Los casos con antecedentes de sífilis se presentaron con menor frecuencia como sífilis primaria (27,5% vs. 40%) (aOR, 0,58 [IC del 95%, 0,44-0,77]). Un año después del tratamiento, la evolución clínica y serológica fue similar en los pacientes VIH positivo y negativo. Conclusiones: La epidemia de sífilis en Barcelona afecta desproporcionalmente a HSH y está estrechamente relacionada con el VIH. La forma de presentación de la sífilis está influida por el antecedente de sífilis y por el VIH, sin diferencias significativas en cuanto a la evolución un año después del tratamiento


Background: Since 2000, substantial increases in syphilis have been reported in metropolitan areas of Western countries, with increases noted among men who have sex with men (MSM). Clinical manifestations of syphilis might be influenced by concomitant VIH infection and previous episodes of syphilis. The objectives of this study were to describe the epidemiological and clinical characteristics of the cases of syphilis diagnosed in Barcelona. Methods: Retrospective study of cases with early syphilis diagnosed in the referral STI Unit of Barcelona from January 2003 to December 2013. Revision of medical records with structured collection of epidemiological and clinical data. Univariate and multivariate statistical analyses comparing the characteristics of MSM cases with and without VIH infection and with and without previous syphilis. Results: A total of 1702 cases of syphilis (37% primary, 48% secondary and 14% early latent) were diagnosed, 93% of them in MSM. Among MSM 40% were coinfected with VIH, VIH-positive cases were associated with a previous syphilis (aOR, 5.2 [95% CI, 3.32-8.24]) and with unprotected anal intercourse (aOR, 1.75 [95%CI, 1.17-2.63]). Cases with a history of syphilis presented less often with primary syphilis compared to those without it (27.5% vs. 40%) (aOR, 0.58 [95% CI, 0.44-0.77]). One year after treatment, the clinical and serological evolution were similar between VIH-positive and VIH-negative cases. Conclusion: The epidemic of syphilis in Barcelona disproportionately affects MSM and is closelly linked to VIH infection. The presentation of syphilis is influenced by VIH infection and by previous history of syphilis, without significant differences in their evolution after one year of treatment


Assuntos
Humanos , Sífilis/epidemiologia , Epidemias , Diagnóstico Precoce , Síndromes de Imunodeficiência/epidemiologia , Espanha/epidemiologia , Estudos Retrospectivos , Infecções por HIV/complicações , Microscopia , Análise Estatística , Pessoas Transgênero , Minorias Sexuais e de Gênero
20.
PLoS Negl Trop Dis ; 13(12): e0007887, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31869329

RESUMO

Myiasis is the infestation of human tissues by dipterous fly larvae of the class Insecta. Clogmia albipunctatus, family Psychodidae, is one of the most medically important insects that cause human myiasis. The aim of the present study is the morphological identification and the molecular characterization of moth flies causing many cases of urinary myiasis in Egypt, based on sequencing of the mitochondrial DNA of the larvae. Seven urinary samples of patients complaining of urinary symptoms and giving a history of low socioeconomic level were examined. Recovered larvae were identified using light microscopy and SEM. For molecular identification, the mitochondrial genes Cytochrome B (cytB), NADH1, NADH1, and 16S were sequenced and phylogenetically analyzed. The morphological and molecular characterization could accurately diagnose our patients to have C. albipunctatus infestation. Such results provided the initial set of data on the molecular identification and phylogenetic analysis of moth flies based on DNA barcoding in Egypt.


Assuntos
Larva/anatomia & histologia , Larva/genética , Miíase/parasitologia , Psychodidae/anatomia & histologia , Psychodidae/genética , Doenças Urológicas/parasitologia , Adolescente , Animais , Criança , Pré-Escolar , Análise por Conglomerados , Citocromos b/genética , Código de Barras de DNA Taxonômico , DNA Mitocondrial/química , DNA Mitocondrial/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Egito , Feminino , Humanos , Larva/classificação , Masculino , Microscopia , Microscopia Eletrônica de Varredura , NADH Desidrogenase/genética , Filogenia , Psychodidae/classificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Adulto Jovem
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