Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 36.447
Filtrar
1.
Methods Mol Biol ; 2576: 437-451, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36152208

RESUMO

Single-molecule localization microscopy (SMLM) opened new possibilities to study the spatial arrangement of molecular distribution and disease-associated redistribution at a previously unprecedented resolution that was not achievable with optical microscopy approaches. Recent discoveries based on SMLM techniques uncovered specific nanoscale organizational principles of signaling proteins in several biological systems including the chemical synapses in the brain. Emerging data suggest that the spatial arrangement of the molecular players of the endocannabinoid system is also precisely regulated at the nanoscale level in synapses and in other neuronal and glial subcellular compartments. The precise nanoscale distribution pattern is likely to be important to subserve several specific signaling functions of this important messenger system in a cell-type- and subcellular domain-specific manner.STochastic Optical Reconstruction Microscopy (STORM) is an especially suitable SMLM modality for cell-type-specific nanoscale molecular imaging due to its compatibility with traditional diffraction-limited microscopy approaches and classical staining methods. Here, we describe a detailed protocol for STORM imaging in mouse brain tissue samples with a focus on the CB1 cannabinoid receptor, one of the most abundant synaptic receptors in the brain. We also summarize important conceptual and methodical details that are essential for the valid interpretation of single-molecule localization microscopy data.


Assuntos
Endocanabinoides , Microscopia , Animais , Endocanabinoides/metabolismo , Camundongos , Microscopia/métodos , Receptores de Canabinoides , Receptores de Neurotransmissores , Sinapses/metabolismo
2.
Food Chem ; 402: 134229, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36182778

RESUMO

Multi-microscopy techniques and Fourier transform infrared (FT-IR) spectroscopy were used in this study to investigate the intrinsic causes leading to fruit texture difference between two cultivars of oriental melon 'HDB' (crisp) and 'HPM' (mealy). On the histological aspect, orderly arranged regular-shaped cells with tissue natural fracture pattern showed cell rupture in 'HDB' versus loosely arranged irregular-shaped cells with tissue natural fracture pattern showed cell-to-cell separation in 'HPM' of sarcocarp are histological causes for crisp and mealy fruit texture, respectively. On the biochemical aspect, FT-IR spectra (4000-850 cm-1) of sarcocarp tissue cell wall materials (CWM) happened a dramatic change at the mature stage in 'HPM', but not in 'HDB'. Insightly, the lower de-methyl-esterified homogalacturonan (HG) abundance with higher water-soluble pectin (WSP) ratio and lower hemicellulose (HC) content contribute a poor intercellular adhesion in 'HPM' middle lamella (ML) at the mature stage compared to 'HDB'.


Assuntos
Cucumis melo , Cucumis melo/química , Frutas/química , Espectroscopia de Infravermelho com Transformada de Fourier , Microscopia , Pectinas/química , Água/análise
3.
Methods Mol Biol ; 2565: 77-87, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36205888

RESUMO

Recent advances in stimulated emission depletion (STED) microscopy offer an unparalleled avenue to study membrane dynamics of exo- and endocytosis, such as fusion pore opening, pore expansion, constriction, and closure, as well as the membrane transformation from flat-shaped to round-shaped vesicles in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This method can potentially be applied to study other membrane structure dynamics in other cell model system.


Assuntos
Células Cromafins , Microscopia , Animais , Bovinos , Membrana Celular/metabolismo , Endocitose , Vesículas Secretórias/metabolismo
4.
Methods Mol Biol ; 2551: 225-243, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36310206

RESUMO

Tau is an intrinsically disordered protein that binds and stabilizes axonal microtubules (MTs) in neurons of the central nervous system. The binding of Tau to MTs is mediated by its repeat domain and flanking proline-rich domains. The positively charged (basic) C-terminal half of Tau also mediates the assembly Tau into fibrillar aggregates in Alzheimer's disease (AD) and tauopathy brains. In recent years, another assembly form of Tau has been identified: Tau can undergo liquid-liquid phase separation (LLPS), which leads to its condensation into liquid-dense phases, either by complex coacervation with polyanions like heparin or RNA or through "self-coacervation" at high Tau concentrations. Condensation of Tau in the absence of polyanions can be enhanced by the presence of molecular crowding agents in a dilute Tau solution. In vitro experiments using recombinant purified Tau are helpful to study the physicochemical determinants of Tau LLPS, which can then be extrapolated into the cellular context. Tau condensation is a new aspect of Tau biology that may play a role for the initiation of Tau aggregation, but also for its physiological function(s), for example, the binding to microtubules. Here we describe how to study the condensation of Tau in vitro using light microscopy, including fluorescence recovery after photobleaching (FRAP), to assess the shape and molecular diffusion in the condensates, a proxy for the degree of condensate percolation. We also describe turbidity measurements of condensate-containing solutions to assess the overall amount of LLPS and time-resolved dynamic light scattering (trDLS) to study the formation and size of Tau condensates.


Assuntos
Doença de Alzheimer , Proteínas tau , Humanos , Proteínas tau/metabolismo , Microscopia , Difusão Dinâmica da Luz , Doença de Alzheimer/metabolismo
5.
Methods Mol Biol ; 2551: 605-631, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36310228

RESUMO

Uncontrolled assembly/disassembly of physiologically formed liquid condensates is linked to irreversible aggregation. Hence, the quest for understanding protein-misfolding disease mechanism might lie in the studies of protein:nucleic acid coacervation. Several proteins with intrinsically disordered regions as well as nucleic acids undergo phase separation in the cellular context, and this process is key to physiological signaling and is related to pathologies. Phase separation is reproducible in vitro by mixing the target recombinant protein with specific nucleic acids at various stoichiometric ratios and then examined by microscopy and nanotracking methods presented herein. We describe protocols to qualitatively assess hallmarks of protein-rich condensates, characterize their structure using intrinsic and extrinsic dyes, quantify them, and analyze their morphology over time. Analysis by nanoparticle tracking provides information on the concentration and diameter of high-order protein oligomers formed in the presence of nucleic acid. Using the model protein (globular domain of recombinant murine PrP) and DNA aptamers (high-affinity oligonucleotides with 25 nucleotides in length), we provide examples of a systematic screening of liquid-liquid phase separation in vitro.


Assuntos
Aptâmeros de Nucleotídeos , Proteínas Intrinsicamente Desordenadas , Nanopartículas , Ácidos Nucleicos , Camundongos , Animais , Microscopia , Proteínas Recombinantes , Proteínas Intrinsicamente Desordenadas/química
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 287(Pt 2): 122087, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36417818

RESUMO

Core-shell nanoparticles have been extensively researched, particularly as multimodal for medical applications. Scientists are interested in combining the optical properties of nano-plasmonic nanoparticles with the magnetic properties of super-paramagnetic nanoparticles. This combination is very important because it reduces metal toxicity and improves nanoparticle targeting. Tuning the shape and size of the nanoparticles significantly reflects their properties and applications. In previous study, we assessed the SPION@Ag@chitosan core-shell nanocomposite (γFe2O3@Ag@Cs NCs) toxicity both in vitro and preclinically in vivo, using traditional toxicological assessment and biochemical parameters. The results showed that up to100 ug/kg is a safe NP dose as evaluated by pathological and biochemical parameters. The aim of the present study was to gain insight into the effect of γFe2O3@Ag@Cs NC at sub-cytotoxic concentrations (100ug/ml) on the biochemical profile of immune organs (inguinal, axillary, spleen and thymus) by combining the investigation of cytokine secretion to ex vivo FTIR spectroscopy. The four immune organs were treated with 100 ug/kg NC and the time dependence of the effects produced by the treatment was analyzed. The Data shows that the used core-shell NC with the indicate dose have a stimulatory effect on the immune system, as evidenced by an increase in antibody secretion (IgG and IgM), lipid, nucleic acid, and protein synthesis after uptake time which depends on the specific immune organ.


Assuntos
Quitosana , Nanocompostos , Espectroscopia de Infravermelho com Transformada de Fourier , Microscopia , Adjuvantes de Vacinas
7.
Spectrochim Acta A Mol Biomol Spectrosc ; 286: 121978, 2023 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-36323081

RESUMO

The role of mitochondria goes beyond their capacity to create molecular fuel and includes e.g. the production of reactive oxygen species and the regulation of cell death. In endothelial cells, mitochondria have a significant impact on cellular function under both healthy and pathological conditions. Endothelial dysfunction contributes to the development of various lifestyle diseases and the key players in their pathogenesis are among others vascular inflammation and oxidative stress. The latter is very closely related to mitochondrial dysfunction; however, it is not straightforward. First, because mitochondria are small cellular structures, and second, it requires a sensitive method to follow the subtle biochemical changes. For this purpose, Raman microscopy (RM) was used here, which is considered a high-resolution method and can be applied in situ, usually as a non-labeled technique. In this work, we show that RM can not only locate mitochondria in the cell but also track their functional changes. Moreover, we test if labeling cells with Raman probes (Rp) can improve the specificity and sensitivity of RM (compared to conventional labeled techniques such as fluorescence, and the non-labeled Raman technique). MitoBADY Rp was used to detect changes in mitochondrial membrane potential as an indicator of mitochondrial activity, e.g. hyperpolarization or distortion of the proton gradient in the intermembrane space (depolarization). Thus, we show and compare RM, in the form of a label and non-labeled, to such a subtle cellular analysis.


Assuntos
Células Endoteliais , Microscopia , Potencial da Membrana Mitocondrial , Células Endoteliais/metabolismo , Mitocôndrias/metabolismo , Espécies Reativas de Oxigênio/metabolismo
8.
Methods Mol Biol ; 2601: 203-229, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36445586

RESUMO

Membrane fluidity is a critical parameter of cellular membranes, which cells continuously strive to maintain within a viable range. Interference with the correct membrane fluidity state can strongly inhibit cell function. Triggered changes in membrane fluidity and associated impacts on lipid domains have been postulated to contribute to the mechanism of action of membrane targeting antimicrobials, but the corresponding analyses have been hampered by the absence of readily available analytical tools. Here, we expand upon the protocols outlined in the first edition of this book, providing further and alternative protocols that can be used to measure changes in membrane fluidity. We provide detailed protocols, which allow straightforward in vivo and in vitro measurement of antibiotic compound-triggered changes in membrane fluidity and fluid membrane microdomains. Furthermore, we summarize useful strains constructed by us and others to characterize and confirm lipid specificity of membrane antimicrobials directly in vivo.


Assuntos
Fluidez de Membrana , Microscopia , Espectrometria de Fluorescência , Membrana Celular , Lipídeos
9.
Methods Mol Biol ; 2601: 231-257, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36445587

RESUMO

Microscopy is a powerful method to evaluate the direct effects of antibiotic action on the single cell level. As with other methodologies, microscopy data is obtained through sufficient biological and technical replicate experiments, where evaluation of the sample is generally followed over time. Even if a single antibiotic is tested for a defined time, the most certain outcome is large amounts of raw data that requires systematic analysis. Although microscopy is a helpful qualitative method, the recorded information is stored as defined quantifiable units, the pixels. When this information is transferred to diverse bioinformatic tools, it is possible to analyze the microscopy data while avoiding the inherent bias associated to manual quantification. Here, we briefly describe methods for the analysis of microscopy images using open-source programs, with a special focus on bacteria exposed to antibiotics.


Assuntos
Bactérias , Microscopia , Antibacterianos/farmacologia , Biologia Computacional , Análise de Sistemas
10.
Methods Mol Biol ; 2601: 171-190, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36445584

RESUMO

The urgent need of new antimicrobial agents to combat life-threatening bacterial infections demands the identification and characterization of novel compounds that interfere with new and unprecedented target pathways or structures in multiresistant bacteria. Here, bacterial cell division has emerged as a new and promising target pathway for antibiotic intervention. Compounds, which inhibit division, commonly induce a characteristic filamentation phenotype of rod-shaped bacteria, such as Bacillus subtilis. Hence, this filamentation phenotype can be used to identify and characterize novel compounds that primarily target bacterial cell division. Since novel compounds of both synthetic and natural product origin are often available in small amounts only, thereby limiting the number of assays during mode of action studies, we here describe a semiautomated, microscopy-based approach that requires only small volumes of compounds to allow for the real-time observation of their effects on living bacteria, such as filamentation or cell lysis, in high-throughput 96-well-based formats. We provide a detailed workflow for the initial characterization of multiple compounds at once and further tools for the initial, microscopy-based characterization of their antibacterial mode of action.


Assuntos
Antibacterianos , Microscopia , Antibacterianos/farmacologia , Bioensaio , Morfogênese , Bacillus subtilis
11.
Appl Radiat Isot ; 191: 110562, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36401988

RESUMO

Radiological, structural, and physical properties of an x-ray oil which absorbed a photon dose of approximately 2.0 ± 0.2 Gy during its operational life (∼10 years), were investigated. An attenuation coefficient of 0.198 ± 0.003 cm-1 (at 122 keV) was obtained. NMR, ESR, and FTIR spectroscopy suggest a preponderance of pentane, and hexane. Mass spectrometry detected tertrapentacontane, nonadecane, and docosane. No gamma-emitters were revealed with gamma spectroscopy. A boiling temperature of approximately 300 °C was measured. Microscopy revealed the presence of gas bubbles.


Assuntos
Unidades Hospitalares , Microscopia , Raios X , Radiografia , Espectrometria de Massas
12.
Methods Mol Biol ; 2596: 465-480, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36378457

RESUMO

Comparative gel electrophoretic analyses of normal versus pathological specimens can swiftly identify proteome-wide changes in the concentration of specific protein isoforms. The application of fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) can be employed for the characterization of complex protein populations in health and disease. In order to verify pathoproteomic findings and correlate them to histopathological alterations, standardized histological and histochemical methodology can be applied for the cell biological analysis of normal versus pathological tissue samples. This chapter outlines the usage of histochemical ATPase staining of fast and slow fiber types in normal versus dystrophic skeletal muscles, as well as the application of hematoxylin and eosin staining of nuclei and the cellular body in kidney cells, and Sudan black staining of lipids in cryo-sections.


Assuntos
Microscopia , Proteoma , Eletroforese em Gel Diferencial Bidimensional/métodos , Proteoma/metabolismo , Músculo Esquelético/metabolismo , Coloração e Rotulagem
13.
J Biomed Opt ; 27(11): 110501, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36458112

RESUMO

Conventional optoacoustic microscopy (OAM) instruments have at their core a nanosecond pulse duration laser. If lasers with a shorter pulse duration are used, broader, higher frequency ultrasound waves are expected to be generated and as a result, the axial resolution of the instrument is improved. Here, we exploit the advantage offered by a picosecond duration pulse laser to enhance the axial resolution of an OAM instrument. In comparison to an instrument equipped with a 2-ns pulse duration laser, an improvement in the axial resolution of 50% is experimentally demonstrated by using excitation pulses of only 85 ps. To illustrate the capability of the instrument to generate high-quality optoacoustic images, en-face, in-vivo images of the brain of Xenopus laevis tadpole are presented with a lateral resolution of 3.8 µ m throughout the entire axial imaging range.


Assuntos
Lasers , Microscopia , Encéfalo , Frequência Cardíaca , Ondas de Rádio
14.
J Chem Phys ; 157(20): 204201, 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36456225

RESUMO

As one of the popular coherent Raman scattering techniques, stimulated Raman scattering (SRS) has made significant progress in recent years, especially in label-free biological imaging. Polarization provides an additional degree of freedom to manipulate the SRS process. In previous studies, only linearly polarized SRS was fully investigated, in which both pump and Stokes laser fields are linearly polarized. Here, we theoretically analyzed the SRS process excited by two circularly polarized laser fields and then experimentally demonstrated it by taking a spherical symmetric CH4 molecule as a model system. The experimental results are in good agreement with the theoretical ones. It is shown that circularly polarized SRS (CP-SRS) has unique characteristics different from linear polarization. When the handedness of circular polarization states of two laser fields is the same, CP-SRS further suppresses the depolarized vibrational band while keeping the polarized band almost unaffected. On the other hand, when the handedness is opposite, CP-SRS enhances the depolarized band while suppressing the polarized band. Therefore, the CP-SRS not only allows us to resolve the symmetry of vibrational modes but also can enhance vibrational contrast based on symmetry selectivity by suppressing or enhancing the signal from a specific vibrational mode. These results will have potential applications in improving chemical selectivity and imaging contrast as well as spectral resolution SRS microscopy. In addition, the CP-SRS has the ability to determine the depolarization ratio ρ and identify the overlapping Raman bands.


Assuntos
Análise Espectral Raman , Vibração , Microscopia , Modelos Biológicos
15.
J Microsc ; 288(3): 153-154, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36420575

Assuntos
Lentes , Microscopia
16.
Mikrochim Acta ; 189(12): 480, 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36445567

RESUMO

A visible and sensitive assay for the quantitative detection of ß-glucosidase (ß-glu) activity based on Au@CeO2 core-shell nanoparticles (Au@CeO2 NPs) is described. As a hydrolytic enzyme, ß-glu can promote the hydrolysis of ß-arbutin to hydroquinone (HQ), which can trigger the decomposition of the CeO2 shell. With the single-particle enumeration (SPE) strategy coupled with dark field optical microscopy (DFM), an obvious color alteration of single Au@CeO2 NPs during the etching process can be observed in real-time. By statistically calculating the number of the etched nanoparticles, the ß-glu activity level can be quantified accurately. This assay displays a broad linear range from 0.5 to 50 mU⋅mL-1 and low detection limit of 0.12 mU⋅mL-1. In addition, this method was successfully used to determine ß-glu in real samples and acquires satisfactory recoveries in the range of 97.1-102.0%. This study provides a visualization analysis method for ß-glu, which may be helpful for monitoring other targets in the future.


Assuntos
Nanopartículas , Bioensaio , beta-Glucosidase , Microscopia , Hidrólise
17.
Opt Express ; 30(23): 42283-42299, 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36366685

RESUMO

Fringe pattern based measurement techniques are the state-of-the-art in full-field optical metrology. They are crucial both in macroscale, e.g., fringe projection profilometry, and microscale, e.g., label-free quantitative phase microscopy. Accurate estimation of the local fringe orientation map can significantly facilitate the measurement process in various ways, e.g., fringe filtering (denoising), fringe pattern boundary padding, fringe skeletoning (contouring/following/tracking), local fringe spatial frequency (fringe period) estimation, and fringe pattern phase demodulation. Considering all of that, the accurate, robust, and preferably automatic estimation of local fringe orientation map is of high importance. In this paper we propose a novel numerical solution for local fringe orientation map estimation based on convolutional neural network and deep learning called DeepOrientation. Numerical simulations and experimental results corroborate the effectiveness of the proposed DeepOrientation comparing it with a representative of the classical approach to orientation estimation called combined plane fitting/gradient method. The example proving the effectiveness of DeepOrientation in fringe pattern analysis, which we present in this paper, is the application of DeepOrientation for guiding the phase demodulation process in Hilbert spiral transform. In particular, living HeLa cells quantitative phase imaging outcomes verify the method as an important asset in label-free microscopy.


Assuntos
Algoritmos , Refratometria , Humanos , Refratometria/métodos , Células HeLa , Microscopia/métodos , Redes Neurais de Computação
18.
Nat Commun ; 13(1): 6918, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36376301

RESUMO

High-throughput measurement of cells perturbed using libraries of small molecules, gene knockouts, or different microenvironmental factors is a key step in functional genomics and pre-clinical drug discovery. However, it remains difficult to perform accurate single-cell assays in 384-well plates, limiting many studies to well-average measurements (e.g., CellTiter-Glo®). Here we describe a public domain Dye Drop method that uses sequential density displacement and microscopy to perform multi-step assays on living cells. We use Dye Drop cell viability and DNA replication assays followed by immunofluorescence imaging to collect single-cell dose-response data for 67 investigational and clinical-grade small molecules in 58 breast cancer cell lines. By separating the cytostatic and cytotoxic effects of drugs computationally, we uncover unexpected relationships between the two. Dye Drop is rapid, reproducible, customizable, and compatible with manual or automated laboratory equipment. Dye Drop improves the tradeoff between data content and cost, enabling the collection of information-rich perturbagen-response datasets.


Assuntos
Antineoplásicos , Descoberta de Drogas , Descoberta de Drogas/métodos , Sobrevivência Celular , Coloração e Rotulagem , Antineoplásicos/farmacologia , Microscopia , Ensaios de Triagem em Larga Escala/métodos
19.
PLoS One ; 17(11): e0277616, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36378676

RESUMO

Woody cells, such as tracheids, fibers, vessels, rays etc., have unique structural characteristics such as nano-scale ultrastructure represented by multilayers, microfibril angle (MFA), micro-scale anatomical properties and spatial arrangement. Simultaneous evaluation of the above indices is very important for their adequate quantification and extracting the effects of external stimuli from them. However, it is difficult in general to achieve the above only by traditional methodologies. To overcome the above point, a new methodological framework combining polarization optical microscopy, fluorescence microscopy, and image segmentation is proposed. The framework was tested to a model softwood species, Chamaecyparis obtusa for characterizing intra-annual transition of MFA and tracheid morphology in a radial file unit. According our result, this framework successfully traced the both characteristics tracheid by tracheid and revealed the high correlation (|r| > 0.5) between S2 microfibril angles and tracheidal morphology (lumen radial diameter, tangential wall thickness and cell wall occupancy). In addition, radial file based evaluation firstly revealed their complex transitional behavior in transition and latewood. The proposed framework has great potential as one of the unique tools to provide detailed insights into heterogeneity of intra and inter-cells in the wide field of view through the simultaneous evaluation of cells' ultrastructure and morphological properties.


Assuntos
Chamaecyparis , Microfibrilas , Microscopia , Madeira , Parede Celular/ultraestrutura
20.
Sensors (Basel) ; 22(21)2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36366194

RESUMO

The most robust and economical method for laboratory diagnosis of tuberculosis (TB) is to identify mycobacteria acid-fast bacilli (AFB) under acid-fast staining, despite its disadvantages of low sensitivity and labor intensity. In recent years, artificial intelligence (AI) has been used in TB-smear microscopy to assist medical technologists with routine AFB smear microscopy. In this study, we evaluated the performance of a TB automated system consisting of a microscopic scanner and recognition program powered by artificial intelligence and machine learning. This AI-based system can detect AFB and classify the level from 0 to 4+. A total of 5930 smears were evaluated on the performance of this automatic system in identifying AFB in daily lab practice. At the first stage, 120 images were analyzed per smear, and the accuracy, sensitivity, and specificity were 91.3%, 60.0%, and 95.7%, respectively. In the second stage, 200 images were analyzed per smear, and the accuracy, sensitivity, and specificity were increased to 93.7%, 77.4%, and 96.6%. After removing disqualifying smears caused by poor staining quality and smear preparation, the accuracy, sensitivity, and specificity were improved to 95.2%, 85.7%, and 96.9%, respectively. Furthermore, the automated system recovered 85 positive smears initially identified as negative by manual screening. Our results suggested that the automated TB system could achieve higher sensitivity and laboratory efficiency than manual microscopy under the quality control of smear preparation. Automated TB smear screening systems can serve as a screening tool at the first screen before manual microcopy.


Assuntos
Mycobacterium tuberculosis , Tuberculose , Humanos , Inteligência Artificial , Tuberculose/diagnóstico , Microscopia/métodos , Coloração e Rotulagem , Sensibilidade e Especificidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...