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1.
An Bras Dermatol ; 94(4): 449-451, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31644619

RESUMO

Benign migratory glossitis or geographic tongue is a benign condition that usually manifests as asymptomatic erythematous and migratory circinate patches, involving the lateral and dorsal aspects of the tongue. Extra-lingual lesions uncommonly occur and are mainly located on labial and buccal mucosae, lips and floor of the mouth. The present report describes one patient with a geographic lesion on the hard palate associated with lingual lesions and another patient who had multiple geographic lesions both in the hard and soft palate without lingual lesions. We found 64 cases in the English literature of ectopic locations with 22 palate involvement. No case of simultaneous involvement of the hard and the soft palate was found.


Assuntos
Glossite Migratória Benigna/patologia , Palato/patologia , Estomatite/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/patologia , Língua/patologia
2.
Zhonghua Liu Xing Bing Xue Za Zhi ; 40(8): 1003-1005, 2019 Aug 10.
Artigo em Chinês | MEDLINE | ID: mdl-31484269

RESUMO

Objective: To investigate the prevalence of oral mucosal diseases (OMD) in patients with cerebrovascular disease. Methods: A total of 182 patients with cerebrovascular disease and 166 controls were examined for OMD to compare the differences of prevalence rates. Results: The prevalence of OMD in patients with cerebrovascular disease appeared higher than that in the control group. Oral candidiasis was most commonly seen (11.1%, 20/182), followed by fissured tongue (5.0%, 9/182), traumatic ulcer (2.8%, 5/182), herpes labialis (2.2%, 4/182), recurrent oral ulcer (1.6%, 3/182), chronic cheilitis (1.6%, 3/182) and oral leukokeratosis (1.6%, 3/182). Conclusion: Patients with cerebrovascular diseases were susceptible to OMDs, especially to oral candidiasis that called for more attention.


Assuntos
Transtornos Cerebrovasculares/epidemiologia , Doenças da Boca/epidemiologia , Mucosa Bucal/patologia , Estudos de Casos e Controles , China/epidemiologia , Humanos , Leucoplasia Oral/epidemiologia , Prevalência , Língua Fissurada/epidemiologia
3.
J Biol Regul Homeost Agents ; 33(3 Suppl. 1): 11-17, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31538445

RESUMO

In vivo Reflectance Confocal Microscopy (RCM) allows to optically biopsy vital tissues, non-invasively and in real time. It results in horizontal virtual slices at a microscopic resolution and correlating with conventional histopathology. The aim of the present work is to describe RCM cellular and architectural findings in oral mucosae affected by erosive-ulcerative diseases, thus highlighting in vivo the wellknown histological peculiarities. A series of conventionally diagnosed Recurrent Aphthous stomatitis (RAS) and Pemphigus Vulgaris (PV) erosive and/or ulcerative oral lesions underwent RCM imaging to establish the application of RCM imaging to this kind of inflammatory non-tumoral lesions. A total of 12 RAS-related lesions and 8 PV-related lesions were considered. RCM imaging was capable to visualize their microscopic peculiarities, mainly inflammatory infiltrate, vessel dilation (RAS) and acantholytic cells, intraepithelial clefts and inflammatory cell carpets (PV). Despite RCM may result unnecessary to diagnose oral lesions referred to RAS and PV, its capability to highlight their main microscopic features could be advantageously used to monitor the healing or worsening of the clinical situation as well as the responsiveness/refractoriness to therapy.


Assuntos
Microscopia Confocal , Mucosa Bucal/diagnóstico por imagem , Mucosa Bucal/patologia , Pênfigo/diagnóstico por imagem , Estomatite Aftosa/diagnóstico por imagem , Biópsia , Humanos
4.
Pan Afr Med J ; 33: 147, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31558944

RESUMO

Pleomorphic Adenoma (PA) is the most common salivary gland tumor and accounts for about 60% of all salivary gland neoplasms. Intraorally, the hard palate is the most common presenting site (50-60%) followed by upper lip (15-20%) and rarely buccal mucosa (8-10%). Histopathologically, PA shows diverse morphology resulting from amalgamation of cellular and stromal components. The PA may show changes in the stromal and epithelial components, such as sebaceous, lipocytic and oncocytic metaplasia. A rare characteristic of PA is to show extensive squamous and mucous differentiation which poses diagnostic dilemma to the pathologist. Here, we present an unusual case of PA of buccal minor salivary gland with squamous and mucous metaplasia. The localization, gender and microscopic features of the presented case are unusual.


Assuntos
Adenoma Pleomorfo/diagnóstico , Mucosa Bucal/patologia , Neoplasias das Glândulas Salivares/diagnóstico , Glândulas Salivares Menores/patologia , Adenoma Pleomorfo/patologia , Adulto , Humanos , Masculino , Metaplasia , Neoplasias das Glândulas Salivares/patologia
5.
Anticancer Res ; 39(8): 4011-4017, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31366482

RESUMO

BACKGROUND/AIM: Genotoxicity is the capacity of an agent to induce damage to DNA. Given the close relationship between genotoxicity and carcinogenesis, several assays have been developed for detecting genetic damage. Among them, the single-cell gel (comet) assay plays an important role for evaluating DNA damage in mammalian cells, including those of the oral cavity. The purpose of this article was to provide a critical review of the application of single-cell gel comet assay to buccal cells. MATERIAL AND METHODS: A search of the scientific literature was conducted of published studies available on single-cell gel comet assay and oral cells. RESULTS: The results showed that the majority of studies were conducted on humans, whereas few were designed for use in rodents and in vitro. CONCLUSION: Further studies within the field are relevant for better understanding the underlying mechanisms of genotoxicity in oral cells, especially since the use of humans is quite complicated due to issues of ethics.


Assuntos
Carcinogênese/genética , Dano ao DNA/genética , Boca/efeitos dos fármacos , Análise de Célula Única/métodos , Ensaio Cometa/métodos , DNA/genética , Humanos , Boca/patologia , Mucosa Bucal/metabolismo , Mucosa Bucal/patologia , Mutagênicos
6.
Cancer Sci ; 110(9): 2783-2793, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31325403

RESUMO

Oral cancer, a subtype of head and neck cancer, is characterized by increased infiltrating regulatory T cells (Treg); however, the pathological significance of the increase in Tregs in disease prognosis and progression and their underlying mechanism remain unestablished. C-C motif chemokine ligand 22 (CCL22) has been implicated in the recruitment of Tregs. We used RT-qPCR to determine CCL22 mRNA expression in clinical specimens and cultured cells. Loss-of-function and gain-of-function studies were carried out to analyze the effects of CCL22 modulations on cell proliferation, migration, invasion, and tumorigenesis and the mechanism involved in the deregulation of CCL22. In oral cancer specimens, CCL22 mRNA was upregulated. The increase was not only associated with reduced disease-free survival but also strongly correlated with an increase in FOXP3 mRNA, a master regulator of Treg development and functions. Silencing CCL22 expression reduced cell proliferation, migration, and invasion, whereas ectopic overexpression showed opposite effects. Manipulation of CCL22 expression in cancer cells altered tumorigenesis in both immune-compromised and -competent mice, supporting both autonomous and non-autonomous actions of CCL22. Release of interleukin 1ß (IL-1ß) from cancer-associated fibroblasts (CAF) induces CCL22 mRNA expression in oral cancer cells by activating transcription factor nuclear factor kappa B (NF-κB). Our data support a model in which CAF-derived IL-1ß, CCL22, and its receptor CCR4 foster a protumor environment by promoting cell transformation and Treg infiltration. Intervention of the IL-1ß-CCL22-CCR4 signaling axis may offer a novel therapeutic strategy for oral cancer treatment.


Assuntos
Fibroblastos Associados a Câncer/metabolismo , Quimiocina CCL22/metabolismo , Interleucina-1beta/metabolismo , Neoplasias Bucais/patologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Animais , Fibroblastos Associados a Câncer/imunologia , Linhagem Celular Tumoral , Movimento Celular/imunologia , Transformação Celular Neoplásica/imunologia , Transformação Celular Neoplásica/patologia , Quimiocina CCL22/genética , Intervalo Livre de Doença , Feminino , Seguimentos , Fatores de Transcrição Forkhead/metabolismo , Técnicas de Silenciamento de Genes , Humanos , Interleucina-1beta/genética , Masculino , Camundongos , Pessoa de Meia-Idade , Mucosa Bucal/patologia , Mucosa Bucal/cirurgia , Neoplasias Bucais/imunologia , Neoplasias Bucais/mortalidade , Neoplasias Bucais/cirurgia , Invasividade Neoplásica/imunologia , Invasividade Neoplásica/patologia , Prognóstico , RNA Interferente Pequeno/metabolismo , Receptores CCR4/metabolismo , Transdução de Sinais/imunologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/imunologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/mortalidade , Carcinoma de Células Escamosas de Cabeça e Pescoço/cirurgia , Análise de Sobrevida , Linfócitos T Reguladores/imunologia , Linfócitos T Reguladores/metabolismo , Regulação para Cima , Ensaios Antitumorais Modelo de Xenoenxerto
7.
Rev Assoc Med Bras (1992) ; 65(6): 870-879, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31340319

RESUMO

This study aimed to assess the prevalence of medical and nonmedical use of psychiatric medication among undergraduate students of health sciences from a public university in Brasil. Another objective was to determine the frequency of nuclear morphological abnormalities in the buccal mucosa of students using psychiatric drugs. A cross-sectional study based on a Web survey was carried out with 375 health sciences undergraduate students from schools of Pharmacy, Physical Education, Nutrition, and Medicine. Additionally, spontaneous genetic damages in exfoliated cells of the buccal mucosa of 41 individuals by counting micronucleus (MN) and binucleated (BN) cells frequencies were evaluated. The results showed 76 (20.3%) of students reported the use of psychotropic drugs after enrolling in university. The majority of these students were from Pharmacy and Medicine programs, females, aged between 18-25 years old, nonsmokers, alcohol addicts, and with a family history of mental illness. In addition, Medical students, individuals with high-income, who live alone and are in the last period of the program are more likely to use psychotropic drugs. Moreover, exposure to psychiatric medication was able to increase the number of binucleated cells. These results provide evidence that the use of psychoactive drugs is increased in the academic context and may be related to the failure of the cell cycle.


Assuntos
Uso Indevido de Medicamentos/estatística & dados numéricos , Mucosa Bucal/efeitos dos fármacos , Mucosa Bucal/patologia , Psicotrópicos/uso terapêutico , Estudantes de Ciências da Saúde/estatística & dados numéricos , Adolescente , Adulto , Brasil , Estudos Transversais , Feminino , Humanos , Masculino , Testes para Micronúcleos , Fatores de Risco , Fatores Socioeconômicos , Inquéritos e Questionários , Universidades , Adulto Jovem
8.
J Drugs Dermatol ; 18(5): 448-453, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31141850

RESUMO

Background: Studies have identified numerous genetic polymorphisms associated with increased risk of melanoma and non-melanoma skin cancer (NMSC). In this pilot study, we aimed to examine whether previously identified melanoma and non-melanoma associated single nucleotide polymorphisms (SNPs) which were found to be associated with cutaneous malignancy were also present in a relatively heterogeneous population with a history of skin cancer versus an age and environmental matched controls. The undertaking of this project serves to further the current understanding of the genetic profile for those at higher risk for developing skin cancer. Methods: Nineteen NMSC patients and their age-matched and environmental controls underwent genotyping of 7 previously discovered SNPs associated with melanoma and NMSC. Results: In a random, heterogeneous population in Southern California, SNP's Chr1, PAD16, PIGU, TDG had a similar association with NMSC previously reported in prior studies. Due to small trial size, no conclusions or observable associations could be drawn from the SNPs MC1R, TP53, and XRCC1. Conclusion: This data supports that 4 of the 7 SNP's studied had similar associations and could potentially be predictive tool of NMSC risk in this patient population. The remaining three SNP's did not have a definitive association with malignancy. Larger studies are needed to further elucidate the specific roles of these SNPs collectively and ultimately to develop a genetic profile for those patients at increased risk of developing skin cancer. J Drugs Dermatol. 2019;18(5):448-453.


Assuntos
Predisposição Genética para Doença , Melanoma/epidemiologia , Mucosa Bucal/patologia , Polimorfismo de Nucleotídeo Único/genética , Neoplasias Cutâneas/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores , California/epidemiologia , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Melanoma/etiologia , Melanoma/genética , Pessoa de Meia-Idade , Projetos Piloto , Valor Preditivo dos Testes , Estudos Prospectivos , Fatores de Risco , Neoplasias Cutâneas/etiologia , Neoplasias Cutâneas/genética , Manejo de Espécimes , Adulto Jovem
9.
Indian J Cancer ; 56(2): 107-113, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31062727

RESUMO

Background: The global incidence of oral cancer occurs in low-resource settings. Community-based oral screening is a strategic step toward downstaging oral cancer by early diagnosis. The mobile health (mHealth) program is a technology-based platform, steered with the aim to assess the use of mHealth by community health workers (CHWs) in the identification of oral mucosal lesions. MATERIALS AND METHODS: mHealth is a mobile phone-based oral cancer-screening program in a workplace setting. The participants were screened by two CHWs, followed by an assessment by an oral medicine specialist. A mobile phone-based questionnaire that included the risk assessment was distributed among participants. On specialist recommendation an oral surgeon performed biopsy on participants. The diagnosis by onsite specialist that was confirmed by histopathology was considered as gold standard. All individuals received the standard treatment protocol. A remote oral medicine specialist reviewed the uploaded data in Open Medical Record System. Sensitivity, specificity, positive and negative predictive values were calculated. Inter-rater agreement was analyzed with Cohen's kappa coefficient (κ) test, and the diagnostic ability of CHWs, onsite specialist, and remote specialist was illustrated using receiver operating characteristic curve. RESULTS: CHWs identified oral lesions in 405 (11.8%) individuals; the onsite specialist identified oral lesions in 394 (11.4%) individuals; and the remote specialist diagnosed oral lesions in 444 (13%). The inter-rater agreement between the CHW and the onsite specialist showed almost perfect agreement with the κ score of 0.92, and a substantial agreement between CHW and remote specialist showed a score of 0.62. The sensitivity, specificity, positive and negative predictive values of CHWs in the identification of oral lesion were 84.7, 97.6, 84.8, and 97.7%, respectively. CONCLUSION: The trained CHWs can aid in identifying oral potentially malignant disorders and they can be utilized in oral cancer-screening program mHealth effectively.


Assuntos
Agentes Comunitários de Saúde , Detecção Precoce de Câncer , Neoplasias Bucais/diagnóstico , Telemedicina , Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/patologia , Neoplasias Bucais/patologia , Inquéritos e Questionários , Adulto Jovem
10.
Rev. Odontol. Araçatuba (Impr.) ; 40(2): 43-47, maio/ago. 2019. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1006564

RESUMO

O objetivo deste estudo é descrever o diagnóstico e conduta clínica no tratamento do Fibroma Traumático (FT). O FT é uma lesão proliferativa de natureza benigna que surge na cavidade bucal devido à traumas de repetição, que desencadeiam reações inflamatórias em tecido conjuntivo, causando uma hiperplasia tecidual, geralmente assintomática, podendo interferir na mastigação e na fala causando desconforto ao paciente. Sua prevalência é alta, geralmente em mucosa jugal, linha oclusal, mucosa labial, língua e gengiva. O tratamento consiste na excisão cirúrgica e a confirmação do diagnóstico é dado através de envio para exame histopatológico. Paciente do gênero masculino, 46 anos de idade, procurou atendimento por apresentar nódulo único, exofítico, unilateral de aproximadamente 3 cm em seu diâmetro, localizado na mucosa jugal do lado direito, com bordas regulares e indolor a palpação, apresentando há cerca de 12 meses. Foi submetido a remoção cirúrgica total da lesão e encaminhamento ao laboratório para análise histopatológica para confirmação diagnóstica, além de proservação do caso em 7, 30 e 60 dias. Desta forma, foi possível concluir que a excisão cirúrgica da lesão promove maior conforto, melhora na fala e mastigação, além de um bom reparo tecidual, devolvendo assim, condições de saúde a mucosa bucal e mínimas chances de recidiva(AU)


The objective of this study is to describe the diagnosis and clinical management in the treatment of Traumatic Fibroma (FT). FT is a proliferative lesion of benign nature that arises in the buccal cavity due to repetitive traumas, which trigger inflammatory reactions in connective tissue, causing a tissue hyperplasia, usually asymptomatic, that can interfere in chewing and speech causing discomfort to the patient. Its prevalence is high, usually in jugal mucosa, occlusal line, labial mucosa, tongue and gingiva. The treatment consists of surgical excision and confirmation of the diagnosis is given by sending for histopathological examination. A 46-year-old male patient sought care for having a single, exophytic, unilateral nodule of approximately 3 cm in diameter, located on the right side of the jugal mucosa, with regular borders and painless palpation, presenting about 12 months. He was submitted to total surgical removal of the lesion and sent to the laboratory for histopathological analysis for diagnostic confirmation, in addition to case proservation at 7, 30 and 60 days. In this way, it was possible to conclude that the surgical excision of the lesion promotes greater comfort, improvement in speech and chewing, besides a good tissue repair, thus returning health conditions to the oral mucosa and minimal chances of relapse(AU)


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Fibroma/cirurgia , Cirurgia Bucal , Fibroma , Fibroma/diagnóstico , Fibroma/terapia , Hiperplasia , Mucosa Bucal/patologia
11.
Anticancer Res ; 39(5): 2519-2525, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31092448

RESUMO

BACKGROUND/AIM: Oral cancer screening is important for early detection and early treatment, which help improve survival rates. Biopsy is invasive and painful, while fluorescence visualization is non-invasive, convenient, and real-time, and examinations can be repeated using optical instruments. The purpose of this study was to clarify the usefulness of an optical instrument in oral screening. PATIENTS AND METHODS: A total of 201 patients, who were examined using an optical instrument in our Department between 2017 and 2018, were enrolled in this study. Fluorescence visualization images were analyzed using subjective and objective evaluations. RESULTS: Subjective evaluations for detecting oral cancer and oral epithelial dysplasia offered 83.3% sensitivity and 75.7% specificity. Regarding the objective evaluations for detecting oral cancer and oral epithelial dysplasia, sensitivity and specificity were 47.4% and 72.4% for luminance value, 94.7% and 79.6% for luminance ratio, and 100.0% and 68.0% coefficient of variation. CONCLUSION: Fluorescence visualization using optical instruments is useful for oral cancer screening.


Assuntos
Carcinoma de Células Escamosas/diagnóstico , Mucosa Bucal/diagnóstico por imagem , Neoplasias Bucais/diagnóstico , Lesões Pré-Cancerosas/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma in Situ , Carcinoma de Células Escamosas/diagnóstico por imagem , Carcinoma de Células Escamosas/patologia , Detecção Precoce de Câncer , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/patologia , Neoplasias Bucais/diagnóstico por imagem , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/diagnóstico por imagem , Lesões Pré-Cancerosas/patologia
12.
mSphere ; 4(2)2019 04 03.
Artigo em Inglês | MEDLINE | ID: mdl-30944214

RESUMO

Denture stomatitis (DS) is a condition characterized by inflammation of the oral mucosa in direct contact with dentures and affects a significant number of otherwise healthy denture wearers. Candida-associated DS is predominantly caused by Candida albicans, a dimorphic fungus that readily colonizes and forms biofilms on denture materials. Previous studies showed a requirement for Candida biofilm formation on both palate and dentures in infection and identified fungal morphogenic transcription factors, Efg1 and Bcr1, as key players in DS pathogenesis. While both C. albicans and Candida glabrata are frequently coisolated in mucosal candidiasis, a pathogenic role for C. glabrata in DS remains unknown. Using an established rat model of DS, we sought to determine whether C. glabrata alone or coinoculation with C. albicans establishes colonization and causes palatal tissue damage and inflammation. Rats fitted with custom dentures were inoculated with C. albicans and/or C. glabrata and monitored over a 4-week period for fungal burden (denture/palate), changes in body weight, and tissue damage via lactate dehydrogenase (LDH) release as well as palatal staining by hematoxylin and eosin (H&E) and immunohistochemistry for myeloperoxidase (MPO) as measures of inflammation. C. glabrata colonized the denture/palate similarly to C. albicans In contrast to C. albicans, colonization by C. glabrata resulted in minimal changes in body weight, palatal LDH release, and MPO expression. Coinoculation with both species had no obvious modulation of C. albicans-mediated pathogenic effects. These data suggest that C. glabrata readily establishes colonization on denture and palate but has no apparent role for inducing/enhancing C. albicans pathogenesis in DS.IMPORTANCE Many denture wearers suffer from Candida-associated denture stomatitis (DS), a fungal infection of the hard palate in contact with dentures. Biofilm formation by Candida albicans on denture/palate surfaces is considered a central process in the infection onset. Although Candida glabrata is frequently coisolated with C. albicans, its role in DS pathogenesis is unknown. We show here, using a contemporary rat model that employed a patented intraoral denture system, that C. glabrata established stable colonization on the denture/palate. However, in contrast to C. albicans inoculated rats, rats inoculated with C. glabrata exhibited minimal changes in weight gain or palatal tissue damage. Likewise, coinoculation with the two Candida species resulted in no exacerbation of C. albicans-induced DS pathology. Together, our findings indicate that C. glabrata has no inducing/enhancing role in DS pathogenesis.


Assuntos
Candida glabrata/fisiologia , Dentaduras/microbiologia , Palato/microbiologia , Estomatite sob Prótese/microbiologia , Estomatite sob Prótese/patologia , Animais , Biofilmes , Candida albicans/genética , Candida albicans/isolamento & purificação , Candida glabrata/patogenicidade , Dentaduras/efeitos adversos , Modelos Animais de Doenças , Inflamação , L-Lactato Desidrogenase/metabolismo , Masculino , Mucosa Bucal/microbiologia , Mucosa Bucal/patologia , Palato/patologia , Peroxidase/análise , Ratos
13.
Biomed Res Int ; 2019: 8456342, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30956987

RESUMO

Dental implantology allows replacement of failing teeth providing the patient with a general improvement of health. Unfortunately not all reconstructions succeed, as a consequence of the development of infections of bacterial origin on the implant surface. Surface topography is known to modulate a differential response to bacterial and mammalian cells but topographical measurements are often limited to vertical parameters. In this work we have extended the topographical measurements also to lateral and hybrid parameters of the five most representative implant and prosthetic component surfaces and correlated the results with bacterial and mammalian cell adhesion and proliferation outcomes. Primary human oral gingival fibroblast (gum cells) and the bacterial strains: Streptococcus mutans, Streptococcus sanguinis and Aggregatibacter actinomycetemcomitans, implicated in infectious processes in the oral/implant environment were employed in the presence or absence of human saliva. The results confirm that even though not all the measured surface is available for bacteria to adhere, the overall race for the surface between cells and bacteria is more favourable to the smoother surfaces (nitrided, as machined or lightly acid etched) than to the rougher ones (strong acid etched or sandblasted/acid etched).


Assuntos
Aderência Bacteriana , Implantes Dentários/microbiologia , Fibroblastos , Gengiva , Bactérias Gram-Positivas/crescimento & desenvolvimento , Mucosa Bucal , Adesão Celular , Fibroblastos/metabolismo , Fibroblastos/microbiologia , Fibroblastos/patologia , Gengiva/metabolismo , Gengiva/microbiologia , Gengiva/patologia , Bactérias Gram-Positivas/classificação , Bactérias Gram-Positivas/isolamento & purificação , Humanos , Mucosa Bucal/metabolismo , Mucosa Bucal/microbiologia , Mucosa Bucal/patologia , Propriedades de Superfície
14.
Asian Pac J Cancer Prev ; 20(4): 1109-1112, 2019 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-31030482

RESUMO

Background and aim: Cigarettes, hookah, and tobacco are the most important etiologic factors for oral cancers and dysplastic lesions. This study was undertaken to determine the correlation between hookah use and the percentage of cells with micronucleus, karyorrhexis, karyolysis, and broken egg in the buccal mucosa; and secondly to compare hookah user and non-user in terms of repair index. Materials and methods: The present historical cohort study was carried out on 72 samples taken from 36 hookah users and 36 control subjects. Smear samples were obtained from participants' buccal mucosa for cytological evaluation using Papanicolaou technique. Then, the percentages of cells with micronucleus, karyorrhexis, karyolysis, and broken egg were recorded and the repair index was calculated. Data were analyzed using Mann-Whitney U test. Results: A total of 72 samples taken from 36 hookah users and 36 control subjects were evaluated. The means of micronucleus scores in the buccal mucosa cells of hookah users and controls were 10.7±2.6 and 5.8±2.0, the karyorrhexis scores in the hookah users and controls were 0.1±0.06 and 0.04±0.06, and the karyolysis scores in hookah users and controls were 0.16±0.05 and 0.08±0.06, respectively. These differences were statistically significant between hookah users and controls (P<0.001). The broken egg score was 0.66±0.07 for the hookah users and 0.03±0.04 for the control group, revealing a statistically significant difference (P<0.036). Finally, the repair index values were 0.03±0.01 and 0.05±0.13 in hookah users and controls, respectively. This difference was also significant (P<0.026). Conclusion: The percentages of cells with micronucleus, karyorrhexis, karyolysis, and broken egg in the buccal mucosa of hookah users were significantly higher than those in control group; in addition, the repair index of the buccal mucosa cells in hookah users was significantly lower than that in the control group.


Assuntos
Núcleo Celular/genética , Micronúcleos com Defeito Cromossômico/induzido quimicamente , Mucosa Bucal/patologia , Neoplasias Bucais/induzido quimicamente , Cachimbos de Água/estatística & dados numéricos , Fumar/efeitos adversos , Adulto , Estudos de Casos e Controles , Núcleo Celular/efeitos dos fármacos , Estudos de Coortes , Seguimentos , Humanos , Masculino , Mucosa Bucal/efeitos dos fármacos , Neoplasias Bucais/genética , Neoplasias Bucais/patologia , Prognóstico
15.
Indian J Cancer ; 56(1): 15-18, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30950437

RESUMO

OBJECTIVES: This study was conducted to determine the number and types of oromucosal lesions (OMLs) in relation to tobacco habits in patients who attended the outpatient department. METHODOLOGY: A total of 1730 patients visiting the Department of Oral Medicine and Radiology were interviewed and screened for tobacco habits (smoking and smokeless). Clinical oral examination was conducted with diagnostic instruments using the Color Atlas of Common Oral Diseases as a guide for diagnosis. When clinical features were not diagnostic, a biopsy was done. RESULTS: Of the 1730 outpatients, 975 (56.3% ) individuals used tobacco in one or other forms and 687 (70.4%) of these had OMLs. CONCLUSION: The results of this study provide important information on the prevalence of OMLs in patients seeking dental care. This provides baseline data for future studies on the prevalence of oral lesions in the general population.


Assuntos
Leucoplasia Oral/epidemiologia , Mucosa Bucal/patologia , Neoplasias Bucais/epidemiologia , Tabaco/efeitos adversos , Adolescente , Adulto , Fatores Etários , Feminino , Seguimentos , Humanos , Índia/epidemiologia , Leucoplasia Oral/etiologia , Leucoplasia Oral/patologia , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/etiologia , Neoplasias Bucais/patologia , Prevalência , Prognóstico , Fatores Sexuais , Adulto Jovem
16.
J Dermatol ; 46(6): 531-534, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31021002

RESUMO

Hypereosinophilic syndrome (HES) is often associated with cutaneous manifestations, mostly pruritic lesions, urticaria and angioedema. Mucosal lesions are rarely seen in HES but, when present, are usually the first manifestation of the disease. The clinical presentation may be heterogeneous, including erosions, aphthae or ulcers, and can be easily confused with other mucocutaneous disorders. Here, we present the case of a 64-year-old man with severe chronic erosive oral mucositis simulating pemphigus in which the finding of persistent eosinophilia and elevation of B12 vitamin serum levels raised the suspicion of HES. The FIP1L1-PDGFRA fusion gene (4q12) was detected by fluorescence in situ hybridization and the patient was treated with imatinib mesylate with complete response of the disease.


Assuntos
Síndrome Hipereosinofílica/diagnóstico , Mesilato de Imatinib/uso terapêutico , Proteínas de Fusão Oncogênica/genética , Pênfigo/diagnóstico , Receptor alfa de Fator de Crescimento Derivado de Plaquetas/genética , Estomatite/diagnóstico , Fatores de Poliadenilação e Clivagem de mRNA/genética , Diagnóstico Diferencial , Humanos , Síndrome Hipereosinofílica/tratamento farmacológico , Síndrome Hipereosinofílica/genética , Síndrome Hipereosinofílica/imunologia , Mesilato de Imatinib/farmacologia , Hibridização in Situ Fluorescente , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/imunologia , Mucosa Bucal/patologia , Proteínas de Fusão Oncogênica/antagonistas & inibidores , Receptor alfa de Fator de Crescimento Derivado de Plaquetas/antagonistas & inibidores , Índice de Gravidade de Doença , Estomatite/tratamento farmacológico , Estomatite/genética , Estomatite/imunologia , Resultado do Tratamento , Fatores de Poliadenilação e Clivagem de mRNA/antagonistas & inibidores
17.
PLoS Pathog ; 15(4): e1007717, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-31009520

RESUMO

Infectious complications are a common cause of morbidity and mortality in cancer patients undergoing chemotherapy due to increased risk of oral and gastrointestinal candidiasis, candidemia and septicemia. Interactions between C. albicans and endogenous mucosal bacteria are important in understanding the mechanisms of invasive infection. We published a mouse intravenous chemotherapy model that recapitulates oral and intestinal mucositis, and myelosuppression in patients receiving 5-fluorouracil. We used this model to study the influence of C. albicans on the mucosal bacterial microbiome and compared global community changes in the oral and intestinal mucosa of the same mice. We validated 16S rRNA gene sequencing data by qPCR, in situ hybridization and culture approaches. Mice receiving both 5Fu and C. albicans had an endogenous bacterial overgrowth on the oral but not the small intestinal mucosa. C. albicans infection was associated with loss of mucosal bacterial diversity in both sites with indigenous Stenotrophomonas, Alphaproteobacteria and Enterococcus species dominating the small intestinal, and Enterococcus species dominating the oral mucosa. Both immunosuppression and Candida infection contributed to changes in the oral microbiota. Enterococci isolated from mice with oropharyngeal candidiasis were implicated in degrading the epithelial junction protein E-cadherin and increasing the permeability of the oral epithelial barrier in vitro. Importantly, depletion of these organisms with antibiotics in vivo attenuated oral mucosal E-cadherin degradation and C. albicans invasion without affecting fungal burdens, indicating that bacterial community changes represent overt dysbiosis. Our studies demonstrate a complex interaction between C. albicans, the resident mucosal bacterial microbiota and the host environment in pathogenesis. We shed significant new light on the role of C. albicans in shaping resident bacterial communities and driving mucosal dysbiosis.


Assuntos
Candida albicans/patogenicidade , Candidíase Bucal/etiologia , Disbiose/induzido quimicamente , Fluoruracila/efeitos adversos , Mucosa Intestinal/microbiologia , Mucosa Bucal/microbiologia , Animais , Antimetabólitos/efeitos adversos , Candida albicans/efeitos dos fármacos , Candida albicans/genética , Candidíase Bucal/patologia , Modelos Animais de Doenças , Feminino , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/patologia , Camundongos , Camundongos Endogâmicos C57BL , Mucosa Bucal/efeitos dos fármacos , Mucosa Bucal/patologia
18.
Int J Mol Sci ; 20(7)2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30934722

RESUMO

Chemotherapy and/or head and neck radiotherapy are frequently associated with oral mucositis. Oral pain, odynophagia and dysphagia, opioid use, weight loss, dehydration, systemic infection, hospitalization and introduction of a feeding tube should be mentioned as the main determinated effect of oral mucositis. Oral mucositis leads to a decreased quality of life and an increase in treatment costs. Moreover, oral mucositis is a life-threatening disease. In addition to its own direct life-threatening consequences, it can also lead to a reduced survival due to the discontinuation or dose reduction of anti-neoplasm therapy. There are numerous strategies for the prevention or treatment of oral mucositis; however, their effectiveness is limited and does not correspond to expectations. This review is focused on the ghrelin and obestatin as potentially useful candidates for the prevention and treatment of chemo- or/and radiotherapy-induced oral mucositis.


Assuntos
Hormônios Gastrointestinais/uso terapêutico , Grelina/uso terapêutico , Estomatite/tratamento farmacológico , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Hormônios Gastrointestinais/farmacologia , Grelina/farmacologia , Humanos , Mucosa Bucal/efeitos dos fármacos , Mucosa Bucal/patologia , Substâncias Protetoras/farmacologia , Substâncias Protetoras/uso terapêutico , Estomatite/patologia
19.
Rev. Odontol. Araçatuba (Impr.) ; 40(1): 52-55, jan.-abr. 2019. tab, graf
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-995188

RESUMO

A grande quantidade de patologias com características clínicas semelhantes possibilita a coexistência de vários diagnósticos diferenciais frente a uma única lesão na mucosa bucal. Muitas vezes a realização de exames complementares, como a biópsia, torna-se mandatória para confirmação do diagnóstico sugestivo. Realizar levantamento dos laudos histopatológicos provenientes de biópsias executadas em uma clínica-escola odontológica no período entre 2011 e 2018. A partir dos prontuários odontológicos, as informações foram coletadas e tabuladas. Foram determinadas a frequência de cada lesão em relação ao total e ao seu próprio grupo de doenças e as características dos indivíduos. Um total de 106 diagnósticos de 105 pacientes (idade média 47,5 anos), sendo a maioria do sexo feminino (68 indivíduos - 64,8%) e leucoderma (67 indivíduos - 63,8%), foram avaliados. Em relação aos grupos de doenças, o mais representativo foi o das neoplasias benignas epiteliais ou mesenquimais (35 casos - 33%), seguido pelo dos processos proliferativos não neoplásicos (30 casos - 28,3%). Quanto aos diagnósticos, foram observados 25 diferentes, nos quais se destacaram o fibroma (30 casos - 28,3%) e a hiperplasia fibrosa inflamatória (22 casos - 20,8%). Não foi constatada nenhuma patologia maligna. Traçar o perfil epidemiológico de certo grupo de indivíduos pode muitas vezes facilitar os diagnósticos, além de auxiliar na implementação de medidas de prevenção e na orientação dos assuntos a serem abordados nos cursos das instituições de ensino superior(AU)


A large number of pathologies with similar clinical features may allow coexistence of several differential diagnoses in a single lesion on the buccal mucosa. According to the clinical picture, complementary exams such as biopsy are often mandatory to confirm the suggestive diagnosis. To carry out a survey of histopathological diagnoses from biopsies performed in a dental school clinic in the period from 2011 to 2018. Information from dental records was gathered and tabulated. The frequency of each lesion was determined in relation to the total and its own group of diseases as well as the epidemiological characteristics of the individuals. A total of 106 diagnoses from 105 patients (mean age 47.5 years) were evaluated, being the majority of them female (68 individuals - 64.8%) and white (67 individuals - 63.8%). In relation to the groups of diseases, epithelial or mesenchymal benign neoplasms (35 cases - 33%) were the most representative, followed by nonneoplastic proliferative processes (30 cases - 28.3%). Regarding the diagnoses, 25 different ones were observed, in which fibroma (30 cases - 28.3%) and inflammatory fibrous hyperplasia (22 cases - 20.8%) were more prevalent. No malignant pathology was found. To describe the epidemiological profile of a population can often facilitate diagnoses, besides assisting in the implementation of preventive measures and in the definition of graduate and post-graduate courses' scope(AU)


Assuntos
Doenças Estomatognáticas/patologia , Mucosa Bucal/lesões , Mucosa Bucal/patologia , Biópsia , Doenças Estomatognáticas/epidemiologia
20.
Rev Assoc Med Bras (1992) ; 65(3): 330-332, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30994828

RESUMO

Vitiligo is the most common depigmenting, chronic acquired disease of the skin and mucosa. However, vitiligo of an unclassified type and mucosal subtype affecting only one area of the mucosa is considered quite uncommon. The diagnosis of vitiligo, regardless of its type, is clinical. Nonetheless, a device that allows the visualization of the tissue fluorescence may be useful for confirming the diagnosis. We present the use of wide-field optical fluorescence device for complementary examination and diagnosis of unusual cases of mucosal vitiligo located only in angles of the mouth.


Assuntos
Doenças da Boca/diagnóstico por imagem , Mucosa Bucal/diagnóstico por imagem , Imagem Óptica/métodos , Vitiligo/diagnóstico por imagem , Fluorescência , Humanos , Masculino , Pessoa de Meia-Idade , Doenças da Boca/patologia , Mucosa Bucal/patologia , Imagem Óptica/instrumentação , Vitiligo/patologia
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